OPENAIRE OpenAire Sygma UnpayWall ZENODO DOAJ Crossref Microsoft Academic Graph Europe PubMed Central Vrije Universiteit Amsterdam (VU Amsterdam) – Research Portal PubMed Central European Union Open Data Portal https://onlinelibrary.wiley.com/doi/pdf/10.1002/advs.201901408 https://doi.org/10.1002/advs.201901408 Advanced Science Open Access 2019-09-19 Abstract<p>Biofouling proceeds in successive steps where the primary colonizers affect the phylogenetic and functional structure of a future microbial consortium. Using microbiologically influenced corrosion (MIC) as a study case, a novel approach for material surface protection is described, which does not prevent biofouling, but rather shapes the process of natural biofilm development to exclude MIC￢ﾀﾐrelated microorganisms. This approach interferes with the early steps of natural biofilm formation affecting how the community is finally developed. It is based on a multilayer artificial biofilm, composed of electrostatically modified bacterial cells, producing antimicrobial compounds, extracellular antimicrobial polyelectrolyte matrix, and a water￢ﾀﾐproof rubber elastomer barrier. The artificial biofilm is constructed layer￢ﾀﾐby￢ﾀﾐlayer (LBL) by manipulating the electrostatic interactions between microbial cells and material surfaces. Field testing on standard steel coupons exposed in the sea for more than 30 days followed by laboratory analyses using molecular￢ﾀﾐbiology tools demonstrate that the preapplied artificial biofilm affects the phylogenetic structure of the developing natural biofilm, reducing phylogenetic diversity and excluding MIC￢ﾀﾐrelated bacteria. This sustainable solution for material protection showcases the usefulness of artificially guiding microbial evolutionary processes via the electrostatic modification and controlled delivery of bacterial cells and extracellular matrix to the exposed material surfaces.</p 0301 basic medicine 0303 health sciences GREENERProjectH2020 Science Q layer‐by‐layer (LBL) Full Papers layer-by-layer (LBL) 6. Clean water polyelectrolytes 03 medical and health sciences 13. Climate action nanolayers SDG 14 - Life Below Water 14. Life underwater 16S rRNA metagenomic bacteria Rijavec, Tomaž, Zrimec, Jan, van Spanning, Rob, Lapanje, Aleš, 2019-09-19 journalpaper Abstract<p>Biofouling proceeds in successive steps where the primary colonizers affect the phylogenetic and functional structure of a future microbial consortium. Using microbiologically influenced corrosion (MIC) as a study case, a novel approach for material surface protection is described, which does not prevent biofouling, but rather shapes the process of natural biofilm development to exclude MIC￢ﾀﾐrelated microorganisms. This approach interferes with the early steps of natural biofilm formation affecting how the community is finally developed. It is based on a multilayer artificial biofilm, composed of electrostatically modified bacterial cells, producing antimicrobial compounds, extracellular antimicrobial polyelectrolyte matrix, and a water￢ﾀﾐproof rubber elastomer barrier. The artificial biofilm is constructed layer￢ﾀﾐby￢ﾀﾐlayer (LBL) by manipulating the electrostatic interactions between microbial cells and material surfaces. Field testing on standard steel coupons exposed in the sea for more than 30 days followed by laboratory analyses using molecular￢ﾀﾐbiology tools demonstrate that the preapplied artificial biofilm affects the phylogenetic structure of the developing natural biofilm, reducing phylogenetic diversity and excluding MIC￢ﾀﾐrelated bacteria. This sustainable solution for material protection showcases the usefulness of artificially guiding microbial evolutionary processes via the electrostatic modification and controlled delivery of bacterial cells and extracellular matrix to the exposed material surfaces.</p Natural Microbial Communities Can Be Manipulated by Artificially Constructed Biofilms 10.1002/advs.201901408 826312