CORDIS OpenAire Sygma Bielefeld Academic Search Engine (BASE) UnpayWall Crossref University of East Anglia digital repository Microsoft Academic Graph OpenAPC Global Initiative Europe PubMed Central PubMed Central European Union Open Data Portal https://ueaeprints.uea.ac.uk/id/eprint/63998/4/Published_manuscript.pdf http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.13842/fullpdf https://doi.org/10.1111/1462-2920.13842 Environmental Microbiology Open Access 2017-06-27 Summary<p>Approximately one￢ﾀﾐthird of volatile organic compounds (VOCs) emitted to the atmosphere consists of isoprene, originating from the terrestrial and marine biosphere, with a profound effect on atmospheric chemistry. However, isoprene provides an abundant and largely unexplored source of carbon and energy for microbes. The potential for isoprene degradation in marine and estuarine samples from the Colne Estuary, UK, was investigated using DNA￢ﾀﾐStable Isotope Probing (DNA￢ﾀﾐSIP). Analysis at two timepoints showed the development of communities dominated by Actinobacteria including members of the genera Mycobacterium, Rhodococcus, Microbacterium and Gordonia. Representative isolates, capable of growth on isoprene as sole carbon and energy source, were obtained from marine and estuarine locations, and isoprene￢ﾀﾐdegrading strains of Gordonia and Mycobacterium were characterised physiologically and their genomes were sequenced. Genes predicted to be required for isoprene metabolism, including four￢ﾀﾐcomponent isoprene monooxygenases (IsoMO), were identified and compared with previously characterised examples. Transcriptional and activity assays of strains growing on isoprene or alternative carbon sources showed that growth on isoprene is an inducible trait requiring a specific IsoMO. This study is the first to identify active isoprene degraders in estuarine and marine environments using DNA￢ﾀﾐSIP and to characterise marine isoprene￢ﾀﾐdegrading bacteria at the physiological and molecular level.</p> 0301 basic medicine 570 Volatile Organic Compounds 0303 health sciences 550 Base Sequence 610 QR Microbiology Sequence Analysis, DNA Environment 6. Clean water Mixed Function Oxygenases Mycobacterium 03 medical and health sciences Hemiterpenes 13. Climate action Pentanes Butadienes Rhodococcus 14. Life underwater Gordonia Bacterium Research Articles Genome, Bacterial GE Environmental Sciences Johnston, Antonia, Crombie, Andrew T., El Khawand, Myriam, Sims, Leanne, Whited, Gregg M., McGenity, Terry J., Murrell, J. Colin, 2017-07-21 journalpaper Summary<p>Approximately one￢ﾀﾐthird of volatile organic compounds (VOCs) emitted to the atmosphere consists of isoprene, originating from the terrestrial and marine biosphere, with a profound effect on atmospheric chemistry. However, isoprene provides an abundant and largely unexplored source of carbon and energy for microbes. The potential for isoprene degradation in marine and estuarine samples from the Colne Estuary, UK, was investigated using DNA￢ﾀﾐStable Isotope Probing (DNA￢ﾀﾐSIP). Analysis at two timepoints showed the development of communities dominated by Actinobacteria including members of the genera Mycobacterium, Rhodococcus, Microbacterium and Gordonia. Representative isolates, capable of growth on isoprene as sole carbon and energy source, were obtained from marine and estuarine locations, and isoprene￢ﾀﾐdegrading strains of Gordonia and Mycobacterium were characterised physiologically and their genomes were sequenced. Genes predicted to be required for isoprene metabolism, including four￢ﾀﾐcomponent isoprene monooxygenases (IsoMO), were identified and compared with previously characterised examples. Transcriptional and activity assays of strains growing on isoprene or alternative carbon sources showed that growth on isoprene is an inducible trait requiring a specific IsoMO. This study is the first to identify active isoprene degraders in estuarine and marine environments using DNA￢ﾀﾐSIP and to characterise marine isoprene￢ﾀﾐdegrading bacteria at the physiological and molecular level.</p> Identification and characterisation of isoprene-degrading bacteria in an estuarine environment 10.1111/1462-2920.13842