IMPACT4SOIL OpenAire HAL INRAE Bielefeld Academic Search Engine (BASE) UnpayWall ProdInra Crossref Microsoft Academic Graph https://hal.science/halsde-00722571/file/Cantarel_gcb12_1.pdf https://doi.org/10.1111/j.1365-2486.2012.02692.x Global Change Biology Open Access 2012-03-10 2020-05-29 Abstract<p>Emissions of the trace gas nitrous oxide (N2O) play an important role for the greenhouse effect and stratospheric ozone depletion, but the impacts of climate change on N2O fluxes and the underlying microbial drivers remain unclear. The aim of this study was to determine the effects of sustained climate change on field N2O fluxes and associated microbial enzymatic activities, microbial population abundance and community diversity in an extensively managed, upland grassland. We recorded N2O fluxes, nitrification and denitrification, microbial population size involved in these processes and community structure of nitrite reducers (nirK) in a grassland exposed for 4ￂﾠyears to elevated atmospheric CO2 (+200ￂﾠppm), elevated temperature (+3.5ￂﾠￂﾰC) and reduction of summer precipitations (￢ﾈﾒ20%) as part of a long￢ﾀﾐterm, multifactor climate change experiment. Our results showed that both warming and simultaneous application of warming, summer drought and elevated CO2 had a positive effect on N2O fluxes, nitrification, N2O release by denitrification and the population size of N2O reducers and NH4 oxidizers. In situ N2O fluxes showed a stronger correlation with microbial population size under warmed conditions compared with the control site. Specific lineages of nirK denitrifier communities responded significantly to temperature. In addition, nirK community composition showed significant changes in response to drought. Path analysis explained more than 85% of in situ N2O fluxes variance by soil temperature, denitrification activity and specific denitrifying lineages. Overall, our study underlines that climate￢ﾀﾐinduced changes in grassland N2O emissions reflect climate￢ﾀﾐinduced changes in microbial community structure, which in turn modify microbial processes.</p> dénitrification Biodiversité et Ecologie 551 AOB diversity Biodiversity and Ecology nosZ [SDV.EE.ECO] Life Sciences [q-bio]/Ecology, environment/Ecosystems nirK Milieux et Changements globaux 2. Zero hunger changement climatique denitrification grasslands N2O prairie 04 agricultural and veterinary sciences 15. Life on land nitrification 6. Clean water [SDE.BE] Environmental Sciences/Biodiversity and Ecology climate change 13. Climate action [SDV.EE.ECO]Life Sciences [q-bio]/Ecology AOB;changement climatique;dénitrification;diversité;prairie;N2O;nitrification 0401 agriculture, forestry, and fisheries [SDE.BE]Environmental Sciences/Biodiversity and Ecology environment/Ecosystems Jean-François Soussana, Thomas Pommier, Amélie A. M. Cantarel, Amélie A. M. Cantarel, Nadine Guillaumaud, Caroline Moirot, Franck Poly, Juliette M. G. Bloor, 2012-05-08 journalpaper Abstract<p>Emissions of the trace gas nitrous oxide (N2O) play an important role for the greenhouse effect and stratospheric ozone depletion, but the impacts of climate change on N2O fluxes and the underlying microbial drivers remain unclear. The aim of this study was to determine the effects of sustained climate change on field N2O fluxes and associated microbial enzymatic activities, microbial population abundance and community diversity in an extensively managed, upland grassland. We recorded N2O fluxes, nitrification and denitrification, microbial population size involved in these processes and community structure of nitrite reducers (nirK) in a grassland exposed for 4ￂﾠyears to elevated atmospheric CO2 (+200ￂﾠppm), elevated temperature (+3.5ￂﾠￂﾰC) and reduction of summer precipitations (￢ﾈﾒ20%) as part of a long￢ﾀﾐterm, multifactor climate change experiment. Our results showed that both warming and simultaneous application of warming, summer drought and elevated CO2 had a positive effect on N2O fluxes, nitrification, N2O release by denitrification and the population size of N2O reducers and NH4 oxidizers. In situ N2O fluxes showed a stronger correlation with microbial population size under warmed conditions compared with the control site. Specific lineages of nirK denitrifier communities responded significantly to temperature. In addition, nirK community composition showed significant changes in response to drought. Path analysis explained more than 85% of in situ N2O fluxes variance by soil temperature, denitrification activity and specific denitrifying lineages. Overall, our study underlines that climate￢ﾀﾐinduced changes in grassland N2O emissions reflect climate￢ﾀﾐinduced changes in microbial community structure, which in turn modify microbial processes.</p> Four Years Of Experimental Climate Change Modifies The Microbial Drivers Of N2o Fluxes In An Upland Grassland Ecosystem 10.1111/j.1365-2486.2012.02692.x