OPENAIRE OpenAire u:cris Permanent Hosting, Archiving and Indexing of Digital Resources and Assets Crossref Europe PubMed Central PubMed Central New Phytologist Open Access 2023-06-29 Summary<p> <p>Bacteria colonize plant roots and engage in reciprocal interactions with their hosts. However, the contribution of individual taxa or groups of bacteria to plant nutrition and fitness is not well characterized due to a lack of inￂﾠsitu evidence of bacterial activity.</p> <p>To address this knowledge gap, we developed an analytical approach that combines the identification and localization of individual bacteria on root surfaces via gold￢ﾀﾐbased inￂﾠsitu hybridization with correlative NanoSIMS imaging of incorporated stable isotopes, indicative of metabolic activity.</p> <p>We incubated Kosakonia strain DS￢ﾀﾐ1￢ﾀﾐassociated, gnotobiotically grown rice plants with 15N￢ﾀﾓN2 gas to detect inￂﾠsitu N2 fixation activity. Bacterial cells along the rhizoplane showedￂﾠheterogeneous patterns of 15N enrichment, ranging from the natural isotope abundance levels up to 12.07 at% 15N (average and median of 3.36 and 2.85 at% 15N, respectively, n￢ﾀﾉ=￢ﾀﾉ697 cells).</p> <p>The presented correlative optical and chemical imaging analysis is applicable to a broad range of studies investigating plant￢ﾀﾓmicrobe interactions. For example, it enables verification of the inￂﾠsitu metabolic activity of host￢ﾀﾐassociated commercialized strains or plant growth￢ﾀﾐpromoting bacteria, thereby disentangling their role in plant nutrition. Such data facilitate the design of plant￢ﾀﾓmicrobe combinations for improvement of crop management.</p> </p 2. Zero hunger 106022 Mikrobiologie rhizosphere bacteria Bacteria plant growth-promoting bacteria plant–microbe interaction Research Oryza biological nitrogen fixation Plants microbial activity in situ hybridization Plant Roots 106026 Ökosystemforschung Rhizosphere 106022 Microbiology NanoSIMS in situ hybridization 106026 Ecosystem research In situ hybridization In Situ Hybridization Soil Microbiology plant-microbe interaction Schmidt, Hannes, Gorka, Stefan, Seki, David, Schintlmeister, Arno, Woebken, Dagmar, 2023-06-28 journalpaper Summary<p> <p>Bacteria colonize plant roots and engage in reciprocal interactions with their hosts. However, the contribution of individual taxa or groups of bacteria to plant nutrition and fitness is not well characterized due to a lack of inￂﾠsitu evidence of bacterial activity.</p> <p>To address this knowledge gap, we developed an analytical approach that combines the identification and localization of individual bacteria on root surfaces via gold￢ﾀﾐbased inￂﾠsitu hybridization with correlative NanoSIMS imaging of incorporated stable isotopes, indicative of metabolic activity.</p> <p>We incubated Kosakonia strain DS￢ﾀﾐ1￢ﾀﾐassociated, gnotobiotically grown rice plants with 15N￢ﾀﾓN2 gas to detect inￂﾠsitu N2 fixation activity. Bacterial cells along the rhizoplane showedￂﾠheterogeneous patterns of 15N enrichment, ranging from the natural isotope abundance levels up to 12.07 at% 15N (average and median of 3.36 and 2.85 at% 15N, respectively, n￢ﾀﾉ=￢ﾀﾉ697 cells).</p> <p>The presented correlative optical and chemical imaging analysis is applicable to a broad range of studies investigating plant￢ﾀﾓmicrobe interactions. For example, it enables verification of the inￂﾠsitu metabolic activity of host￢ﾀﾐassociated commercialized strains or plant growth￢ﾀﾐpromoting bacteria, thereby disentangling their role in plant nutrition. Such data facilitate the design of plant￢ﾀﾓmicrobe combinations for improvement of crop management.</p> </p Gold‐FISH enables targeted NanoSIMS analysis of plant‐associated bacteria 10.1111/nph.19112 https://doi.org/10.1111/nph.19112 636928