CORDIS OpenAire CORE (RIOXX-UK Aggregator) Sygma Bielefeld Academic Search Engine (BASE) UnpayWall DOAJ Crossref University of East Anglia digital repository Microsoft Academic Graph Europe PubMed Central PubMed Central European Union Open Data Portal http://oro.open.ac.uk/77831/1/40793_2021_Article_381.pdf https://ueaeprints.uea.ac.uk/id/eprint/80327/1/Published_Version.pdf https://link.springer.com/content/pdf/10.1186/s40793-021-00381-2.pdf https://doi.org/10.1186/s40793-021-00381-2 Environmental Microbiome Open Access 2021-02-10 2021-06-21 2021-02-09 Abstract<p>Conventional methods of agricultural pest control and crop fertilisation are contributing to a crisis of biodiversity loss, biogeochemical cycle dysregulation, and ecosystem collapse. Thus, we must find ecologically responsible means to control disease and promote crop yields. The root-associated microbiome may contribute to this goal as microbes can aid plants with disease suppression, abiotic stress relief, and nutrient bioavailability. We applied 16S rRNA gene &amp; fungal 18S rRNA gene (ITS2 region) amplicon sequencing to profile the diversity of the bacterial, archaeal &amp; fungal communities associated with the roots of UK elite spring bread wheat varietyTriticum aestivum var.Paragon in different soils and developmental stages. This revealed that community composition shifted significantly for all three groups across compartments. This shift was most pronounced for bacteria and fungi, while we observed weaker selection on the ammonia oxidising archaea-dominated archaeal community. Across multiple soil types we found that soil inoculum was a significant driver of endosphere community composition, however several bacterial families were identified as core enriched taxa in all soil conditions. The most abundant of these wereStreptomycetaceaeandBurkholderiaceae.Moreover, as the plants senesce, both families were reduced in abundance, indicating that input from the living plant was required to maintain their abundance in the endosphere. To understand which microbes are using wheat root exudates in the rhizosphere, root exudates were labelled in a13CO2DNA stable isotope probing experiment. This shows that bacterial taxa within theBurkholderiaceaefamily among other core enriched taxa, such asPseudomonadaceae,were able to use root exudates butStreptomycetaceaewere not. Overall, this work provides a better understanding of the wheat microbiome, including the endosphere community. Understanding crop microbiome formation will contribute to ecologically responsible methods for yield improvement and biocontrol in the future.</p> 2. Zero hunger 0301 basic medicine 570 0303 health sciences Exudate 15. Life on land Senescence Microbiology 630 QR1-502 Environmental sciences 03 medical and health sciences Root Wheat GE1-350 Microbiome Endosphere Research Article Prudence, Samuel MM., Newitt, Jake T., Worsley, Sarah F., Macey, Michael C., Murrell, J. Colin, Lehtovirta-Morley, Laura E., Hutchings, Matthew I., 2021-02-09 journalpaper Abstract<p>Conventional methods of agricultural pest control and crop fertilisation are contributing to a crisis of biodiversity loss, biogeochemical cycle dysregulation, and ecosystem collapse. Thus, we must find ecologically responsible means to control disease and promote crop yields. The root-associated microbiome may contribute to this goal as microbes can aid plants with disease suppression, abiotic stress relief, and nutrient bioavailability. We applied 16S rRNA gene &amp; fungal 18S rRNA gene (ITS2 region) amplicon sequencing to profile the diversity of the bacterial, archaeal &amp; fungal communities associated with the roots of UK elite spring bread wheat varietyTriticum aestivum var.Paragon in different soils and developmental stages. This revealed that community composition shifted significantly for all three groups across compartments. This shift was most pronounced for bacteria and fungi, while we observed weaker selection on the ammonia oxidising archaea-dominated archaeal community. Across multiple soil types we found that soil inoculum was a significant driver of endosphere community composition, however several bacterial families were identified as core enriched taxa in all soil conditions. The most abundant of these wereStreptomycetaceaeandBurkholderiaceae.Moreover, as the plants senesce, both families were reduced in abundance, indicating that input from the living plant was required to maintain their abundance in the endosphere. To understand which microbes are using wheat root exudates in the rhizosphere, root exudates were labelled in a13CO2DNA stable isotope probing experiment. This shows that bacterial taxa within theBurkholderiaceaefamily among other core enriched taxa, such asPseudomonadaceae,were able to use root exudates butStreptomycetaceaewere not. Overall, this work provides a better understanding of the wheat microbiome, including the endosphere community. Understanding crop microbiome formation will contribute to ecologically responsible methods for yield improvement and biocontrol in the future.</p> Soil, senescence and exudate utilisation: characterisation of the Paragon var. spring bread wheat root microbiome 10.1186/s40793-021-00381-2