OPENAIRE OpenAire Datacite Sygma Bielefeld Academic Search Engine (BASE) Recolector de Ciencia Abierta, RECOLECTA Repositorio Institucional de la Universidad de Oviedo ZENODO Crossref Repositorio Institucional de la Universidad de Burgos Europe PubMed Central European Union Open Data Portal SSRN Electronic Journal Open Access 2022-05-28 2022-07-08 In the present work, the operational conditions for improving the degradation rates of Total Petroleum Hydrocarbons (TPHs) in contaminated soil from a machinery park were optimized at a microcosms scale along a 90- days incubation period. In this study, bioremediation strategies and an organic amendment have been tested to verify the remediation of soil contaminated with different hydrocarbons, mineral oils, and heavy metals. Specifically, designed biostimulation and bioaugmentation strategies were compared with and without adding vermicompost. The polluted soil harboring multiple contaminants, partially attenuated for years, was used. The initial profile showed enrichment in heavy linear alkanes, suggesting a previous moderate weathering. The application of vermicompost increased five and two times the amounts of available phosphorus (P) and exchangeable potassium (K), respectively, as a direct consequence of the organic amendment addition. The microbial activity increased due to soil acidification, which influenced the solubility of P and other micronutrients. It also impacted the predominance and variability of the different microbial groups and the incubation, as reflected by phospholipid fatty acid (PLFA) results. An increase in the alkaline phosphatases and proteases linked to bacterial growth was displayed. This stimulation of microbial metabolism correlated with the degradation rates since TPHs degradation’ efficiency after vermicompost addition reached 32.5% and 34.4% of the initial hydrocarbon levels for biostimulation and bioaugmentation, respectively. Although Polycyclic Aromatic Soil enzymes 01 natural sciences Total petroleum hydrocarbons polluted soils Soil Bioaugmentation Alkanes Soil Pollutants Micronutrients Polycyclic Aromatic Hydrocarbons Materials Phospholipids Soil Microbiology 0105 earth and related environmental sciences 2. Zero hunger Minerals Materiales Biostimulation, Bioaugmentation, Phospholipid fatty acids, Total petroleum hydrocarbons polluted soils, Soil enzymes Fatty Acids Phosphorus Phenanthrenes Hydrocarbons Phosphoric Monoester Hydrolases 6. Clean water Biostimulation Biodegradation, Environmental Petroleum 13. Climate action Phospholipid fatty acids Potassium Oils Biostimulation Bioaugmentation Phospholipid fatty acids Total petroleum hydrocarbons polluted soils Soil enzymes Peptide Hydrolases Sandra Curiel-Alegre, Blanca Velasco-Arroyo, Carlos Rumbo-Lorenzo, Aqib Hassan Ali Khan, Juan Antonio Tamayo-Ramos, Carlos Rad-Moradillo, Jose Luis R. Gallego, Rocio Barros-Garcia, 2022-01-01 journalpaper In the present work, the operational conditions for improving the degradation rates of Total Petroleum Hydrocarbons (TPHs) in contaminated soil from a machinery park were optimized at a microcosms scale along a 90- days incubation period. In this study, bioremediation strategies and an organic amendment have been tested to verify the remediation of soil contaminated with different hydrocarbons, mineral oils, and heavy metals. Specifically, designed biostimulation and bioaugmentation strategies were compared with and without adding vermicompost. The polluted soil harboring multiple contaminants, partially attenuated for years, was used. The initial profile showed enrichment in heavy linear alkanes, suggesting a previous moderate weathering. The application of vermicompost increased five and two times the amounts of available phosphorus (P) and exchangeable potassium (K), respectively, as a direct consequence of the organic amendment addition. The microbial activity increased due to soil acidification, which influenced the solubility of P and other micronutrients. It also impacted the predominance and variability of the different microbial groups and the incubation, as reflected by phospholipid fatty acid (PLFA) results. An increase in the alkaline phosphatases and proteases linked to bacterial growth was displayed. This stimulation of microbial metabolism correlated with the degradation rates since TPHs degradation’ efficiency after vermicompost addition reached 32.5% and 34.4% of the initial hydrocarbon levels for biostimulation and bioaugmentation, respectively. Although Polycyclic Aromatic Evaluation of Biostimulation, Bioaugmentation, and Organic Amendments Application on the Bioremediation of Recalcitrant Hydrocarbons of Soil 10.2139/ssrn.4106102 https://doi.org/10.2139/ssrn.4106102 826312