OPENAIRE OpenAire Datacite CORE (RIOXX-UK Aggregator) Research@WUR Sygma Bielefeld Academic Search Engine (BASE) Repository of the Czech Academy of Sciences Publikationenserver der Georg-August-Universität Göttingen Crossref Europe PubMed Central PubMed Central European Union Open Data Portal https://pnas.org/doi/pdf/10.1073/pnas.2201072119 https://doi.org/11104/0341036 Proceedings of the National Academy of Sciences Open Access 2022-07-18 <p> Soil compaction represents a major agronomic challenge, inhibiting root elongation and impacting crop yields. Roots use ethylene to sense soil compaction as the restricted air space causes this gaseous signal to accumulate around root tips. Ethylene inhibits root elongation and promotes radial expansion in compacted soil, but its mechanistic basis remains unclear. Here, we report that ethylene promotes abscisic acid (ABA) biosynthesis and cortical cell radial expansion. Rice mutants of ABA biosynthetic genes had attenuated cortical cell radial expansion in compacted soil, leading to better penetration. Soil compaction-induced ethylene also up-regulates the auxin biosynthesis gene OsYUC8 . Mutants lacking OsYUC8 are better able to penetrate compacted soil. The auxin influx transporter OsAUX1 is also required to mobilize auxin from the root tip to the elongation zone during a root compaction response. Moreover, osaux1 mutants penetrate compacted soil better than the wild-type roots and do not exhibit cortical cell radial expansion. We conclude that ethylene uses auxin and ABA as downstream signals to modify rice root cell elongation and radial expansion, causing root tips to swell and reducing their ability to penetrate compacted soil. </p roots 0301 basic medicine 570 Cell biology Arabidopsis Biophysics Plant Science Plant Roots Biochemistry Gene Catalysis Mixed Function Oxygenases Molecular Mechanisms of Plant Development and Regulation soil compaction Agricultural and Biological Sciences Soil Abscisic acid Ethylene 03 medical and health sciences aba ethylene Auxin Elongation Biology Plant Proteins 580 2. Zero hunger 0303 health sciences Multidisciplinary Indoleacetic Acids Mutant Life Sciences Oryza Plant Nutrient Uptake and Signaling Pathways Biological Sciences Ethylenes 15. Life on land Materials science Root Aeration Chemistry ABA Plant Responses to Flooding Stress Ultimate tensile strength Mutation Metallurgy auxin Abscisic Acid Guoqiang Huang, Azad Kilic, Michal Karády, Jiao Zhang, Poonam Mehra, Xiaoyun Song, Craig J. Sturrock, Wanwan Zhu, Hua Qin, Sjon Hartman, H Schneider, Rahul Bhosale, Ian C. Dodd, Robert E. Sharp, Rongfeng Huang, Sacha J. Mooney, Wanqi Liang, Malcolm J. Bennett, Dabing Zhang, Bharati Pandey, 2022-07-18 journalpaper <p> Soil compaction represents a major agronomic challenge, inhibiting root elongation and impacting crop yields. Roots use ethylene to sense soil compaction as the restricted air space causes this gaseous signal to accumulate around root tips. Ethylene inhibits root elongation and promotes radial expansion in compacted soil, but its mechanistic basis remains unclear. Here, we report that ethylene promotes abscisic acid (ABA) biosynthesis and cortical cell radial expansion. Rice mutants of ABA biosynthetic genes had attenuated cortical cell radial expansion in compacted soil, leading to better penetration. Soil compaction-induced ethylene also up-regulates the auxin biosynthesis gene OsYUC8 . Mutants lacking OsYUC8 are better able to penetrate compacted soil. The auxin influx transporter OsAUX1 is also required to mobilize auxin from the root tip to the elongation zone during a root compaction response. Moreover, osaux1 mutants penetrate compacted soil better than the wild-type roots and do not exhibit cortical cell radial expansion. We conclude that ethylene uses auxin and ABA as downstream signals to modify rice root cell elongation and radial expansion, causing root tips to swell and reducing their ability to penetrate compacted soil. </p Ethylene inhibits rice root elongation in compacted soil via ABA- and auxin-mediated mechanisms 11104/0341036 891262