OPENAIRE OpenAire Datacite Sygma LAReferencia - Red Federada de Repositorios Institucionales de Publicaciones Científicas Latinoamericanas DOAJ Crossref CONICET Digital Europe PubMed Central PubMed Central https://www.nature.com/articles/s41598-023-49194-y.pdf https://doi.org/11336/226991 Scientific Reports Open Access 2023-12-13 Abstract<p>Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16￢ﾀﾓC17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892ￂﾠbp, with a total of 3474 coding sequences and a G￢ﾀﾉ+￢ﾀﾉC content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1￢ﾀﾓ4 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16￢ﾀﾓC17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.</p 0301 basic medicine Bacteriocin Science Bacillus . Gene Synteny Microbiology Article Agricultural and Biological Sciences Lipopeptides 03 medical and health sciences https://purl.org/becyt/ford/1.6 Biochemistry, Genetics and Molecular Biology Genetics Escherichia coli RNA Sequencing Data Analysis https://purl.org/becyt/ford/1 Molecular Biology Biology Phylogeny GC-content 2. Zero hunger 0303 health sciences Genome Acinetobacter Bacteria Secondary metabolites Q Probiotics and Prebiotics In silico R Life Sciences Anti-Bacterial Agents 3. Good health Ribosomal RNA Whole genome sequencing FOS: Biological sciences Medicine Microbial Enzymes and Biotechnological Applications Antibacterial activity Genome, Bacterial metagenomics assembly Biotechnology Food Science Phylogenetic tree Daniela B. Medeot, Analía Inés Sannazzaro, María Julia Estrella, Gonzalo Torres Tejerizo, Bruno Contreras‐Moreira, Mariano Pistorio, Edgardo Jofré, 2023-12-13 journalpaper Abstract<p>Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16￢ﾀﾓC17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892ￂﾠbp, with a total of 3474 coding sequences and a G￢ﾀﾉ+￢ﾀﾉC content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1￢ﾀﾓ4 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16￢ﾀﾓC17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.</p Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis 11336/226991 101091255