Recognizing existing materials that can act as proxies for Earth's building blocks, and understanding the accretionary pathway taken during Earth's growth, persist as outstanding issues in need of resolution. In Mahan, Siebert, Blanchard, Badro, et al. (2018, https://doi.org/10.1029/2018JB015991), we conducted diamond anvil cell (DAC) Cu metal\uffe2\uff80\uff90silicate partitioning experiments and coupled these results with a large complement of literature data to characterize Cu metal\uffe2\uff80\uff90silicate partitioning during Earth's core formation and accretion history. The Comment of Jennings, Wade, and Llovet (2019, https://doi.org/10.1029/2018JB016930) contends that secondary X\uffe2\uff80\uff90ray fluorescence, originating from the Cu holders that experiments are routinely welded to (\uffe2\uff80\uff9clift\uffe2\uff80\uff90out\uffe2\uff80\uff9d grids), compromises the novel Cu partitioning data of Mahan, Siebert, Blanchard, Badro, et al. (2018) beyond utility. To dispel these concerns and further validate our data, we have (i) investigated secondary X\uffe2\uff80\uff90ray fluorescence effects in a Cu\uffe2\uff80\uff90free experiment and provided a matrix\uffe2\uff80\uff90matched data correction, and (ii) rewelded a DAC experiment from a Cu grid to a Mo grid for a comparison of compositional analyses and Cu partitioning results. Secondary fluorescence results, in fact much like the simulated results in Jennings, Wade, and Llovet (2019), indicate that this effect is essentially equal in the metal and silicate phases and therefore has no actual impact on Cu metal\uffe2\uff80\uff90silicate partition coefficients. Moreover, Cu concentrations and partition coefficients determined using the Mo grid are statistically indistinguishable from that determined using the Cu grid. All results decisively illustrate that while secondary X\uffe2\uff80\uff90ray fluorescence must be considered where absolute concentrations are the final objective, it has had no meaningful impact on the partitioning data and observations of Mahan, Siebert, Blanchard, Badro, et al. (2018).
", "keywords": ["[SDU] Sciences of the Universe [physics]", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "550", "diamond anvil cell", "13. Climate action", "copper", "metal-silicate partitioning", "modeling", "core formation", "geochemistry"]}, "links": [{"href": "https://agupubs.onlinelibrary.wiley.com/doi/pdf/10.1029/2019JB017599"}, {"href": "https://doi.org/10.1029/2019jb017599"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Geophysical%20Research%3A%20Solid%20Earth", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1029/2019jb017599", "name": "item", "description": "10.1029/2019jb017599", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1029/2019jb017599"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-12-01T00:00:00Z"}}, {"id": "10.1038/ncomms8617", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:18:36Z", "type": "Journal Article", "created": "2015-07-03", "title": "Extensive volatile loss during formation and differentiation of the Moon", "description": "AbstractLow estimated lunar volatile contents, compared with Earth, are a fundamental observation for Earth\uffe2\uff80\uff93Moon system formation and lunar evolution. Here we present zinc isotope and abundance data for lunar crustal rocks to constrain the abundance of volatiles during the final stages of lunar differentiation. We find that ferroan anorthosites are isotopically heterogeneous, with some samples exhibiting high \uffce\uffb466Zn, along with alkali and magnesian suite samples. Since the plutonic samples were formed in the lunar crust, they were not subjected to degassing into vacuum. Instead, their compositions are consistent with enrichment of the silicate portions of the Moon in the heavier Zn isotopes. Because of the difference in \uffce\uffb466Zn between bulk silicate Earth and lunar basalts and crustal rocks, the volatile loss likely occurred in two stages: during the proto-lunar disk stage, where a fraction of lunar volatiles accreted onto Earth, and from degassing of a differentiating lunar magma ocean, implying the possibility of isolated, volatile-rich regions in the Moon\uffe2\uff80\uff99s interior.
", "keywords": ["2. Zero hunger", "ISOTOPE FRACTIONATION", "ORIGIN", "IRON", "COPPER", "01 natural sciences", "Article", "[SDU] Sciences of the Universe [physics]", "ZINC", "ABUNDANCES", "13. Climate action", "LUNAR VOLCANIC GLASSES", "WATER", "EARTH", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.nature.com/articles/ncomms8617.pdf"}, {"href": "https://doi.org/10.1038/ncomms8617"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/ncomms8617", "name": "item", "description": "10.1038/ncomms8617", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/ncomms8617"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-07-03T00:00:00Z"}}, {"id": "10.1093/femsec/fiy212", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:19:30Z", "type": "Journal Article", "created": "2018-10-22", "title": "Do soil bacterial communities respond differently to abrupt or gradual additions of copper?", "description": "ABSTRACTMany experiments that measure the response of microbial communities to heavy metals increase metal concentrations abruptly in the soil. However, it is unclear whether abrupt additions mimic the gradual and often long-term accumulation of these metals in the environment where microbial populations may adapt. In a greenhouse experiment that lasted 26 months, we tested whether bacterial communities and soil respiration differed between soils that received an abrupt or a gradual addition of copper or no copper at all. Bacterial richness and other diversity indices were consistently lower in the abrupt treatment compared to the ambient treatment that received no copper. The abrupt addition of copper yielded different initial bacterial communities than the gradual addition; however, these communities appeared to converge once copper concentrations were approximately equal. Soil respiration in the abrupt treatment was initially suppressed but recovered after four months. Afterwards, respiration in both the gradual and abrupt treatments wavered between being below or equal to the ambient treatment. Overall, our study indicates that gradual and abrupt additions of copper can yield similar bacterial communities and respiration, but these responses may drastically vary until copper concentrations are equal.
", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Bacteria", "Microbiota", "Bacterial Physiological Phenomena", "Soil", "03 medical and health sciences", "13. Climate action", "Metals", " Heavy", "Soil Pollutants", "Copper", "Ecosystem", "Soil Microbiology"]}, "links": [{"href": "http://academic.oup.com/femsec/article-pdf/95/1/fiy212/29189709/fiy212.pdf"}, {"href": "https://doi.org/10.1093/femsec/fiy212"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/femsec/fiy212", "name": "item", "description": "10.1093/femsec/fiy212", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/femsec/fiy212"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-10-23T00:00:00Z"}}, {"id": "10.1093/plcell/koac215", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:19:33Z", "type": "Journal Article", "created": "2022-07-22", "title": "Energy status-promoted growth and development of Arabidopsis require copper deficiency response transcriptional regulator SPL7", "description": "AbstractCopper (Cu) is a cofactor of around 300 Arabidopsis proteins, including photosynthetic and mitochondrial electron transfer chain enzymes critical for adenosine triphosphate (ATP) production and carbon fixation. Plant acclimation to Cu deficiency requires the transcription factor SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE7 (SPL7). We report that in the wild type (WT) and in the spl7-1 mutant, respiratory electron flux via Cu-dependent cytochrome c oxidase is unaffected under both normal and low-Cu cultivation conditions. Supplementing Cu-deficient medium with exogenous sugar stimulated growth of the WT, but not of spl7 mutants. Instead, these mutants accumulated carbohydrates, including the signaling sugar trehalose 6-phosphate, as well as ATP and NADH, even under normal Cu supply and without sugar supplementation. Delayed spl7-1 development was in agreement with its attenuated sugar responsiveness. Functional TARGET OF RAPAMYCIN and SNF1-RELATED KINASE1 signaling in spl7-1 argued against fundamental defects in these energy-signaling hubs. Sequencing of chromatin immunoprecipitates combined with transcriptome profiling identified direct targets of SPL7-mediated positive regulation, including Fe SUPEROXIDE DISMUTASE1 (FSD1), COPPER-DEFICIENCY-INDUCED TRANSCRIPTION FACTOR1 (CITF1), and the uncharacterized bHLH23 (CITF2), as well as an enriched upstream GTACTRC motif. In summary, transducing energy availability into growth and reproductive development requires the function of SPL7. Our results could help increase crop yields, especially on Cu-deficient soils.Concerns over the possible increase in phytoavailability of biosolids\uffe2\uff80\uff90applied trace metals to plants have been raised based on the assumption that decomposition of applied organic matter would increase phytoavailability. The objectives of this study were to assess the effect of time on chemical extractability and concentration of Cd, Cu, Ni, and Zn in plants on plots established by a single application of biosolids with high trace metals content in 1984. Biosolids were applied to 1.5 by 2.3 m confined plots of a Davidson clay loam (clayey, kaolinitic, thermic Rhodic Kandiudults) at 0, 42, 84, 126, 168, and 210 Mg ha\uffe2\uff88\uff921 The highest biosolids application supplied 4.5, 760, 43, and 620 kg ha\uffe2\uff88\uff921 of Cd, Cu, Ni, and Zn, respectively. Radish (Raphanus sativus L.), romaine lettuce (Lactuca sativa L. varlongifolia), and barley (Hordeum vulgare L.) were planted at the site for 3 consecutive years, 17 to 19 yr after biosolids application. Extractable Cd, Cu, Ni, and Zn (as measured by DTPA, CaCl2, and Mehlich\uffe2\uff80\uff901) were determined on 15\uffe2\uff80\uff90cm depth samples from each plot. The DTPA\uffe2\uff80\uff90extractable Cu and Zn decreased by 58 and 42%, respectively, 17 yr after application despite a significant reduction in organic matter content. Biosolids treatments had no significant effect on crop yield. Plant tissue metal concentrations increased with biosolids rate but were within the normal range of these crops. Trace metal concentrations in plants generally correlated well with the concentrations extracted from soil with DTPA, CaCl2, and Mehlich\uffe2\uff80\uff901. Metal concentrations in plant tissue exhibited a plateau response in most cases. The uptake coefficient values generated for the different crops were in agreement with the values set by the Part 503 Rule.
", "keywords": ["2. Zero hunger", "Virginia", "Biological Availability", "Hordeum", "04 agricultural and veterinary sciences", "Pentetic Acid", "15. Life on land", "01 natural sciences", "6. Clean water", "Raphanus", "Refuse Disposal", "Trace Elements", "Calcium Chloride", "Soil", "Zinc", "Nickel", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Copper", "Cadmium", "Lactuca", "0105 earth and related environmental sciences"], "contacts": [{"organization": "L. W. Zelazny, Beshr Sukkariyah, Gregory K. Evanylo, Rufus L. Chaney,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.2134/jeq2004.0369"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2004.0369", "name": "item", "description": "10.2134/jeq2004.0369", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2004.0369"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-11-01T00:00:00Z"}}, {"id": "10.3389/fpls.2021.688318", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:22:21Z", "type": "Journal Article", "created": "2021-10-11", "title": "Involvement of Arabidopsis Multi-Copper Oxidase-Encoding LACCASE12 in Root-to-Shoot Iron Partitioning: A Novel Example of Copper-Iron Crosstalk", "description": "Numerous central biological processes depend on the participation of the essential elements iron (Fe) or copper (Cu), including photosynthesis, respiration, cell wall remodeling and oxidative stress protection. Yet, both Fe and Cu metal cations can become toxic when accumulated in excess. Because of the potent ligand-binding and redox chemistries of these metals, there is a need for the tight and combined homeostatic control of their uptake and distribution. Several known examples pinpoint an inter-dependence of Fe and Cu homeostasis in eukaryotes, mostly in green algae, yeast and mammals, but this is less well understood in multicellular plants to date. In Arabidopsis, Cu deficiency causes secondary Fe deficiency, and this is associated with reduced in vitro ferroxidase activity and decreased root-to-shoot Fe translocation. Here we summarize the current knowledge of the cross-talk between Cu and Fe homeostasis and present a partial characterization of LACCASE12 (LAC12) that encodes a member of the multicopper oxidase (MCO) protein family in Arabidopsis. LAC12 transcript levels increase under Fe deficiency. The phenotypic characterization of two mutants carrying T-DNA insertions suggests a role of LAC12 in root-to-shoot Fe partitioning and in maintaining growth on Fe-deficient substrates. A molecular understanding of the complex interactions between Fe and Cu will be important for combating Fe deficiency in crops and for advancing biofortification approaches.Copper (Cu) and its based preparations have been used for over 200\uffc2\uffa0years to control fungi and bacterial diseases in cultivated plants. Downy mildew caused by the obligate biotrophic oomycete Plasmopara viticola is one of the most relevant and recurrent diseases of grapevines. Recently, the use of Cu is being limited by some regulations because of its high impact at different levels (health and environmental problems). Due to its accumulation in soil, this metal causes a little controversy with the principles of sustainable production. Therefore, international legislation and initiatives have recently been arisen to start limiting its use, with the main goal to replace it. In this framework, some alternatives have been tested and others are recently being developed to replace, at least partially, the use of Cu in viticulture. Many of them, are being developed and tested under the scope of research and development EU funded projects. To not compromise sustainability targets in viticulture, results from these R&D projects need to be considered to assess the present risks of using Cu in viticulture and to better support establishing limits for its applications, considering soils vulnerability, while no sustainable alternatives are available in the market.
", "keywords": ["2. Zero hunger", "13. Climate action", "9. Industry and infrastructure", "634", "15. Life on land", "Copper", "12. Responsible consumption"]}, "links": [{"href": "http://www.intechopen.com/chapter/pdf-download/78970"}, {"href": "https://publicatt.unicatt.it/bitstream/10807/215429/2/78970.pdf"}, {"href": "http://www.intechopen.com/download/pdf/78970"}, {"href": "https://doi.org/10.5772/intechopen.100500"}, {"rel": "self", "type": "application/geo+json", "title": "10.5772/intechopen.100500", "name": "item", "description": "10.5772/intechopen.100500", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5772/intechopen.100500"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-06-15T00:00:00Z"}}, {"id": "10261/270558", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:26:48Z", "type": "Journal Article", "created": "2021-12-03", "title": "Corrigendum: Involvement of Arabidopsis Multi-Copper Oxidase-Encoding LACCASE12 in Root-to-Shoot Iron Partitioning: A Novel Example of Copper-Iron Crosstalk", "description": "2 Pags.- 1 Fig. \u00a9 2021 Bernal and Kr\u00e4mer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). A Corrigendum on Involvement of Arabidopsis Multi-Copper Oxidase-Encoding LACCASE12 in Root-to-Shoot Iron Partitioning: A Novel Example of Copper-Iron Crosstalk / by Bernal, M., and Kr\u00e4mer, U. (2021). Front. Plant Sci. 12:688318. doi: 10.3389/fpls.2021.688318. In the original article, there was a mistake in Figure 2B as published. The panel B showed the positive control of panel A and it was accidentally taken from a different experiment when the figure was prepared. The corrected Figure 2 with the correct positive control plate appears below. Peer reviewed", "keywords": ["iron", "multicopper oxidase", "copper", "homeostasis", "Plant culture", "deficiency", "Plant Science", "SB1-1110"]}, "links": [{"href": "https://doi.org/10261/270558"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10261/270558", "name": "item", "description": "10261/270558", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10261/270558"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-12-03T00:00:00Z"}}, {"id": "10045/143865", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:26:40Z", "type": "Journal Article", "created": "2024-06-17", "title": "In-between Frame and Gallery: Framing and installing as architectural practice", "description": "Open AccessBu makale, bir mimar\u0131n sergi ve m\u00fcze projelerine kat\u0131l\u0131m\u0131 \u00fczerine teorik bir inceleme sunmakta, sergi yap\u0131m\u0131n\u0131 alternatif bir mimari uygulama olarak anlamak i\u00e7in bir temel olu\u015fturmay\u0131 ama\u00e7lamaktad\u0131r. \u00c7er\u00e7eveleme ve yerle\u015ftirmeyi birer ara\u00e7 olarak tarifleyerek mimar\u0131n sergi alan\u0131na kat\u0131l\u0131m\u0131n\u0131n kolektif \u00fcretim s\u00fcre\u00e7lerine nas\u0131l katk\u0131 sa\u011flad\u0131\u011f\u0131n\u0131 incelemektedir. Sergi yap\u0131m\u0131n\u0131n fiziksel ve entelekt\u00fcel alanlar\u0131nda mimar\u0131n incelikli konumu, bireylerle, kurumlarla, malzemelerle ve mekanlarla ili\u015fkilerdeki s\u00fcrekli d\u00f6n\u00fc\u015f\u00fcmler yoluyla ortaya \u00e7\u0131kar. Makale, sergileme prati\u011findeki farkl\u0131 kat\u0131l\u0131m bi\u00e7imleriyle ili\u015fkili olarak bir mimar\u0131n kendisini sanatsal ve mimari \u00fcretim aras\u0131ndaki e\u015fikte nas\u0131l konumland\u0131rabilece\u011fini ara\u015ft\u0131rmaktad\u0131r. Ayn\u0131 m\u00fczede e\u015fzamanl\u0131 olarak d\u00fczenlenen iki sergiye odaklanan metin, birinde mimar aktif olarak geri \u00e7ekildi\u011fi, di\u011ferinde mimari tasar\u0131mda proaktif bir rol \u00fcstlendi\u011fi birbirine z\u0131t iki yakla\u015f\u0131m\u0131 \u00f6n plana \u00e7\u0131karmay\u0131 ama\u00e7lamaktad\u0131r.\u00c7al\u0131\u015fma, mimarl\u0131k ve sanat s\u0131n\u0131r\u0131nda faaliyet g\u00f6steren sergilerin \u00fcretiminde \u00e7er\u00e7eveyi birincil ara\u00e7 olarak, \u00e7er\u00e7eveleme ve yerle\u015ftirmeyi ise birincil eylemler olarak oda\u011f\u0131na almaktad\u0131r.", "keywords": ["Distance", "Proximity", "Installation", "Frame", "Exhibition space"], "contacts": [{"organization": "Do\u011fan Taupitz, Duygu, \u015eenel, Asl\u0131han,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10045/143865"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/UOU%20scientific%20journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10045/143865", "name": "item", "description": "10045/143865", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10045/143865"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "10261/256035", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:26:47Z", "type": "Journal Article", "created": "2021-10-11", "title": "Involvement of Arabidopsis Multi-Copper Oxidase-Encoding LACCASE12 in Root-to-Shoot Iron Partitioning: A Novel Example of Copper-Iron Crosstalk", "description": "Numerous central biological processes depend on the participation of the essential elements iron (Fe) or copper (Cu), including photosynthesis, respiration, cell wall remodeling and oxidative stress protection. Yet, both Fe and Cu metal cations can become toxic when accumulated in excess. Because of the potent ligand-binding and redox chemistries of these metals, there is a need for the tight and combined homeostatic control of their uptake and distribution. Several known examples pinpoint an inter-dependence of Fe and Cu homeostasis in eukaryotes, mostly in green algae, yeast and mammals, but this is less well understood in multicellular plants to date. In Arabidopsis, Cu deficiency causes secondary Fe deficiency, and this is associated with reduced in vitro ferroxidase activity and decreased root-to-shoot Fe translocation. Here we summarize the current knowledge of the cross-talk between Cu and Fe homeostasis and present a partial characterization of LACCASE12 (LAC12) that encodes a member of the multicopper oxidase (MCO) protein family in Arabidopsis. LAC12 transcript levels increase under Fe deficiency. The phenotypic characterization of two mutants carrying T-DNA insertions suggests a role of LAC12 in root-to-shoot Fe partitioning and in maintaining growth on Fe-deficient substrates. A molecular understanding of the complex interactions between Fe and Cu will be important for combating Fe deficiency in crops and for advancing biofortification approaches.Copper (Cu) is a cofactor of around 300 Arabidopsis proteins, including photosynthetic and mitochondrial electron transfer chain enzymes critical for adenosine triphosphate (ATP) production and carbon fixation. Plant acclimation to Cu deficiency requires the transcription factor SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE7 (SPL7). We report that in the wild type (WT) and in the spl7-1 mutant, respiratory electron flux via Cu-dependent cytochrome c oxidase is unaffected under both normal and low-Cu cultivation conditions. Supplementing Cu-deficient medium with exogenous sugar stimulated growth of the WT, but not of spl7 mutants. Instead, these mutants accumulated carbohydrates, including the signaling sugar trehalose 6-phosphate, as well as ATP and NADH, even under normal Cu supply and without sugar supplementation. Delayed spl7-1 development was in agreement with its attenuated sugar responsiveness. Functional TARGET OF RAPAMYCIN and SNF1-RELATED KINASE1 signaling in spl7-1 argued against fundamental defects in these energy-signaling hubs. Sequencing of chromatin immunoprecipitates combined with transcriptome profiling identified direct targets of SPL7-mediated positive regulation, including Fe SUPEROXIDE DISMUTASE1 (FSD1), COPPER-DEFICIENCY-INDUCED TRANSCRIPTION FACTOR1 (CITF1), and the uncharacterized bHLH23 (CITF2), as well as an enriched upstream GTACTRC motif. In summary, transducing energy availability into growth and reproductive development requires the function of SPL7. Our results could help increase crop yields, especially on Cu-deficient soils.Copper (Cu) is a cofactor of around 300 Arabidopsis proteins, including photosynthetic and mitochondrial electron transfer chain enzymes critical for adenosine triphosphate (ATP) production and carbon fixation. Plant acclimation to Cu deficiency requires the transcription factor SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE7 (SPL7). We report that in the wild type (WT) and in the spl7-1 mutant, respiratory electron flux via Cu-dependent cytochrome c oxidase is unaffected under both normal and low-Cu cultivation conditions. Supplementing Cu-deficient medium with exogenous sugar stimulated growth of the WT, but not of spl7 mutants. Instead, these mutants accumulated carbohydrates, including the signaling sugar trehalose 6-phosphate, as well as ATP and NADH, even under normal Cu supply and without sugar supplementation. Delayed spl7-1 development was in agreement with its attenuated sugar responsiveness. Functional TARGET OF RAPAMYCIN and SNF1-RELATED KINASE1 signaling in spl7-1 argued against fundamental defects in these energy-signaling hubs. Sequencing of chromatin immunoprecipitates combined with transcriptome profiling identified direct targets of SPL7-mediated positive regulation, including Fe SUPEROXIDE DISMUTASE1 (FSD1), COPPER-DEFICIENCY-INDUCED TRANSCRIPTION FACTOR1 (CITF1), and the uncharacterized bHLH23 (CITF2), as well as an enriched upstream GTACTRC motif. In summary, transducing energy availability into growth and reproductive development requires the function of SPL7. Our results could help increase crop yields, especially on Cu-deficient soils.Numerous central biological processes depend on the participation of the essential elements iron (Fe) or copper (Cu), including photosynthesis, respiration, cell wall remodeling and oxidative stress protection. Yet, both Fe and Cu metal cations can become toxic when accumulated in excess. Because of the potent ligand-binding and redox chemistries of these metals, there is a need for the tight and combined homeostatic control of their uptake and distribution. Several known examples pinpoint an inter-dependence of Fe and Cu homeostasis in eukaryotes, mostly in green algae, yeast and mammals, but this is less well understood in multicellular plants to date. In Arabidopsis, Cu deficiency causes secondary Fe deficiency, and this is associated with reduced in vitro ferroxidase activity and decreased root-to-shoot Fe translocation. Here we summarize the current knowledge of the cross-talk between Cu and Fe homeostasis and present a partial characterization of LACCASE12 (LAC12) that encodes a member of the multicopper oxidase (MCO) protein family in Arabidopsis. LAC12 transcript levels increase under Fe deficiency. The phenotypic characterization of two mutants carrying T-DNA insertions suggests a role of LAC12 in root-to-shoot Fe partitioning and in maintaining growth on Fe-deficient substrates. A molecular understanding of the complex interactions between Fe and Cu will be important for combating Fe deficiency in crops and for advancing biofortification approaches.\u00a0Zn >\u00a0Cu deficiency. We observed a stronger expression of PS pathway genes and greater PS exudation in the barley line with large micronutrient grain yield suggesting that a highly expressed PS pathway might be an important trait involved in high micronutrient accumulation. In addition to several metal specific transporters, we also found that the expression of IRO2 and bHLH156 transcription factors was not only induced under Fe but also under Zn and Cu deficiency. Our study delivers important insights into the role of the PS pathway in the acquisition of different micronutrients.", "keywords": ["2. Zero hunger", "Phytosiderophore", "/dk/atira/pure/subjectarea/asjc/1300/1311", "/dk/atira/pure/subjectarea/asjc/1100/1102", "Root exudation", "name=Genetics", "Iron", "/dk/atira/pure/subjectarea/asjc/1100/1110", "Hordeum", "Copper deficiency", "Plant Roots", "630", "Mugineic acid", "name=Agronomy and Crop Science", "Zinc", "Barley", "Humans", "Micronutrients", "name=Plant Science", "Biofortification", "Copper"]}, "links": [{"href": "https://doi.org/37992897"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "37992897", "name": "item", "description": "37992897", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/37992897"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-02-01T00:00:00Z"}}, {"id": "42de8d2d-b676-4458-aeea-4cc992b2ff55", "type": "Feature", "geometry": {"type": "Polygon", "coordinates": [[[5.72, 47.23], [5.72, 54.62], [15.3, 54.62], [15.3, 47.23], [5.72, 47.23]]]}, "properties": {"updated": "2024-11-07T14:36:37", "type": "Service", "created": "2011-11-28", "language": "ger", "title": "First soil condition survey in the forest (BZE Forest I)", "description": "Die bundesweite Bodenzustandserhebung im Wald (BZE Wald) ist Bestandteil\n des forstlichen Umweltmonitorings. Die BZE I erhob einmalig an ca. 1.800\n Stichprobenpunkten den Zustand von Waldb\u00f6den. Au\u00dfer dem Waldboden\n wurden auch die Baumbestockung und der Kronenzustand untersucht.\n Verkn\u00fcpfungen bestanden teilweise mit ICP Forests Level I und der\n Waldzustandserhebung (WZE).\n\nVerteilung Probenahmestandorte: 8 x 8 km-Raster (in manchen Bundesl\u00e4ndern verdichtet)\n\nProbennahmemethode:\n\u2022 Probenentnahme und Aufbereitung nach BML 1990: Bundesweite Bodenzustandserhebung im Wald (BZE). Arbeitsanleitung, Bonn, Neuauflage 1994\n\u2022 Satellitenbeprobung mit einem Bodenprofil am BZE-Mittelpunkt\n\u2022 Probenahme f\u00fcr die chemischen Analysen nach Tiefenstufen\n\u2022 Methodische Abweichungen einzelner Bundesl\u00e4nder von der gemeinsamen Arbeitsanleitung sind beschrieben in BMELV 2007: Ergebnisse der bundesweiten Bodenzustandserhebung im Wald I, Band 1 (1996, \u00fcberarbeite Version von 2007) http://bfh-web.fh-eberswalde.de/bze/front_content.php?idcat=107&idart=163.\n\nEntnahmetiefe(n):\n\u2022 0 bis 5 cm\n\u2022 5 bis 10 cm\n\u2022 10 bis 30 cm\n\u2022 30 bis 60 cm\n\u2022 60 bis 90 cm\n\u2022 sofern m\u00f6glich auch 90 bis 140 cm, 140 bis 200 cm\n\nUntersuchungsmethode(n):\nAnalyse nach BML 1990: Bundesweite Bodenzustandserhebung im Wald (BZE). Arbeitsanleitung, Bonn, Neuauflage 1994\n\nArbeitsgruppen / Gremien:\nBund-/L\u00e4nder-AG BZE des Bundesministeriums f\u00fcr Ern\u00e4hrung, Landwirtschaft und Verbraucherschutz (BMELV)\n\nR\u00e4umliche Aufl\u00f6sung der bereitgestellten Daten:\n4x4 km (aggregierte Kachel des JRC-Soil-Grids: http://eusoils.jrc.ec.europa.eu/library/reference_grids/reference_grids.cfm )", "formats": [{"name": "OGC:WMS-http-get-capabilities"}], "keywords": ["inspireidentifiziert", "opendata", "infoMapAccessService", "WMS", "National", "BZE", "Bodenzustandserhebung", "Wald", "Bodenfunktion", "Bodenkarte", "Bodennutzbarkeit", "WO", "Wald\u00f6kologie", "Waldinventur", "Bodenmessaktivit\u00e4t", "Aufnahmesituation", "Blattgehalt", "Buche", "Elementvorrat", "Humusstatus", "Kronenzustand", "Kationenaustauschverh\u00e4ltnisse", "Nadelgehalt", "Fichte", "Kiefer", "Bestockungstyp", "Substratgruppe", "Bodentyp", "Podsoligkeit", "H\u00f6he", "pH", "H2O", "KCL", "S\u00e4urebelastungsrisiko", "Kupfer", "Cu", "Calcium", "Ca", "Magnesium", "Mg", "Kalium", "K", "Stickstoff", "N", "Kohlenstoff", "C", "Humusform", "Spurenlemente", "Hauptn\u00e4hrelemente", "C/N", "C/P", "Phosphor", "P", "Aluminium", "Al", "Basens\u00e4ttigung", "Eisen", "Fe", "Elastizit\u00e4t", "Mangan", "Mn", "Wasserstoff", "H+", "Schadstufe", "Verf\u00e4rbung", "Schwefel", "S", "Zink", "Zn", "Blei", "Pb", "Cadmium", "Cd", "Nadeljahrgang", "Elementgehalt", "Bodenfeststoff", "B\u00e4ume", "Baum", "Schwermetallgehalt", "Schwermetallvorrat", "Schwermetallvorr\u00e4te", "Protonens\u00e4ttigung", "Kohlenstoffgehalt", "Kohlenstoffvorrat", "Bodenfeststoff", "Bodenl\u00f6sung", "Krone", "Stamm", "Blatt", "Bl\u00e4tter", "Nadeln", "Boden"], "contacts": [{"name": null, "organization": "Th\u00fcnen-Institut f\u00fcr Wald\u00f6kosysteme", "position": null, "roles": ["pointOfContact"], "phones": [{"value": null}], "emails": [{"value": "geomd-wo@thuenen.de"}], "addresses": [{"deliveryPoint": [null], "city": null, "administrativeArea": null, "postalCode": null, "country": "Deutschland"}], "links": [{"href": {"url": "https://www.thuenen.de/de/wo", "protocol": "WWW:LINK-1.0-http--link", "protocol_url": "", "name": null, "name_url": "", "description": null, "description_url": "", "applicationprofile": null, "applicationprofile_url": "", "function": null}}]}], "themes": [{"concepts": [{"id": "National"}], "scheme": "Spatial scope"}, {"concepts": [{"id": "Boden"}], "scheme": "GEMET - INSPIRE themes, version 1.0"}], "title_alternate": "Darstellungsdienst"}, "links": [{"href": "https://inspire.thuenen.de/geoserver/bze1_wald/ows?service=WMS&version=1.3.0&request=GetCapabilities", "name": "GetCapabilities-Request (WMS)", "description": "GetCapabilities-Dokument (Selbstbeschreibung des Dienstes)", "protocol": "OGC:WMS-http-get-capabilities", "rel": "information"}, {"href": "https://inspire.thuenen.de/geoserver/bze1_wald/ows?service=WMS&version=1.3.0&request=GetCapabilities", "description": "GetCapabilities-Dokument (Selbstbeschreibung des Dienstes)", "protocol": "WWW:LINK-1.0-http--link"}, {"href": "https://gdi-catalog.bmel.de/srv/api/records/42de8d2d-b676-4458-aeea-4cc992b2ff55/attachments/small.png", "name": "preview", "description": "Web image thumbnail (URL)", "protocol": "WWW:LINK-1.0-http--image-thumbnail", "rel": "preview"}, {"rel": "self", "type": "application/geo+json", "title": "42de8d2d-b676-4458-aeea-4cc992b2ff55", "name": "item", "description": "42de8d2d-b676-4458-aeea-4cc992b2ff55", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/42de8d2d-b676-4458-aeea-4cc992b2ff55"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date-time": "2024-11-07T14:36:37Z"}}, {"id": "8d34ddab-2bc9-4288-869b-a4afdd68f0dd", "type": "Feature", "geometry": {"type": "Polygon", "coordinates": [[[9.02, 52.76], [9.02, 52.76], [9.03, 52.76], [9.03, 52.76], [9.02, 52.76]]]}, "properties": {"themes": [{"concepts": [{"id": "farming"}], "scheme": "https://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_TopicCategoryCode"}, {"concepts": [{"id": "Bodenbedeckung"}, {"id": "Bodennutzung"}, {"id": "Landwirtschaftliche Anlagen und Aquakulturanlagen"}], "scheme": "GEMET - INSPIRE themes, version 1.0"}, {"concepts": [{"id": "Shoots"}, {"id": "Plant parts"}, {"id": "nutrient balance"}, {"id": "Avena"}, {"id": "Avena nuda"}, {"id": "Poaceae"}, {"id": "Mustard"}, {"id": "Sinapis alba"}, {"id": "Phacelia tanacetifolia"}, {"id": "Trifolium alexandrinum"}, {"id": ",biomass"}, {"id": "biomass"}, {"id": "biomass"}, {"id": "biomass"}, {"id": "biomass"}, {"id": "biomass"}, {"id": "Elements"}, {"id": "Nitrogen"}, {"id": "Nitrogen content"}, {"id": "Phosphorus"}, {"id": "Carbon"}, {"id": "Magnesium"}, {"id": "Potassium"}, {"id": "Boron"}, {"id": "Aluminium"}, {"id": "Manganese"}, {"id": "Sulphur"}, {"id": "Zinc"}, {"id": "Iron"}, {"id": "Copper"}, {"id": "Calcium"}, {"id": "Catch cropping"}, {"id": "Crop rotation"}, {"id": "cropping systems"}, {"id": "Biological competition"}, {"id": "Interspecific competition"}], "scheme": "AGROVOC Multilingual agricultural thesaurus"}, {"concepts": [{"id": "Shoot biomass"}, {"id": "catch crops"}, {"id": "mineral elements"}, {"id": "macro elements"}, {"id": "micro elements"}, {"id": "C/N ratio"}, {"id": "plant nutrition"}, {"id": "ICP-OES"}, {"id": "EA"}, {"id": "opendata"}], "scheme": "Individual"}, {"concepts": [{"id": "Boden"}], "scheme": "GEMET - INSPIRE themes, version 1.0"}], "rights": "Reports, articles, papers, scientific and non - scientific works of any form, including tables, maps, or any other kind of output, in printed or electronic form, based in whole or in part on the data supplied, must contain an acknowledgement of the form: \"Data reused from the BonaRes Data Centre www.bonares.de. This data were created as part of BonaRes Module A-Project - CATCHY's research activities.\n\nAlthough every care has been taken in preparing and testing the data, BonaRes Module A - Project - CATCHY and BonaRes Data Centre cannot guarantee that the data are correct; neither does BonaRes Module A - Project and BonaRes Data Centre accept any liability whatsoever for any error, missing data or omission in the data, or for any loss or damage arising from its use. The BonaRes Module A-Project-CATCHY and BonaRes Data Centre will not be responsible for any direct or indirect use which might be made of the data. The access to this data is restricted during embargo time. If prior access is requested, contact the data owner / author.", "updated": "2019-06-21", "type": "Dataset", "created": "2017-10-19", "language": "eng", "title": "Catch crop nutrient uptake 1st crop rotation cycle", "description": "A central aspect when including catch crops into a crop rotation is the conservation of nutrients in their biomass for the subsequently grown crop. Therefore, it is important to qualify and to quantify the nutrient accumulation in the biomass of catch crop species. Since it was often described, that mixtures yield higher biomasses than pure stands of catch crops, we evaluated the nutrient scavenging potential of pure stands vs. mixtures. \nTest objects were the four species mustard, phacelia, bristle oat and Egyptian clover either grown in pure stands (sowing densities: mustard - 300, phacelia - 706, bristle oat - 588, Egyptian clover - 833) or in a 4-species mixture (sowing densities: mustard - 67, phacelia - 294, bristle oat - 53, Egyptian clover - 233). Additionally, a commercial mixture of the DSV with a higher species diversity called TerraLife MaisPro was included in the experiment. Their single-species nutrient accumulation was evaluated after 2.5 months of cultivation in total shoot material (dryed for 3 d at 80 \u00b0C and ground in a mill) obtained from two sites in Germany (Asendorf - Lower Saxony and Triesdorf - Bavaria), and at two initial starting points of the respective wheat-catch crop-maize long-term rotation (2015 and 2016) - in total 4 test environments. \nGenerally, nutrient concentrations in the shoot biomass often followed species-specific patterns, e.g. phacelia and oat which are described to have a shallow root system with a high amount of fine roots in the upper soil layers had consistently highest P and K concentrations, S, which is prone to leaching, was most concentrated in the cruciferous species mustard, Ca concentration was highest in phacelia but very low abundant in oat as grass species or Mg was highest in clover since photosynthesis rate must be kept high because biologically fixed N has to be incorporated into carbon skeletons. Increasing interspecific competition in the mix (at higher plant survival rates or at vigorous plant development) favored higher concentrations of several nutrients in some of the species, e.g. higher P concentration in phacelia when cultivated in the 4-species mix. Non-favorable conditions like less water availability led, against this, to higher N concentrations in clover likely due to the establishment of N fixation (Triesdorf 2015 and Asendorf 2016).\nHowever, total nutrient scavenging was largely influenced by the biomass formed by a catch crop variant. In this case, above-ground nutrient conservation capacities were mostly equally high in mustard, phacelia, partially oat and the mixed cultures. Only in one test environment (Triesdorf 2016) where quite loose pure stands established, the mixed cultivation offered a larger nutrient conservation capacity via the production of higher total biomass.", "formats": [{"name": "CSV"}], "keywords": ["Bodenbedeckung", "Bodennutzung", "Landwirtschaftliche Anlagen und Aquakulturanlagen", "Shoots", "Plant parts", "nutrient balance", "Avena", "Avena nuda", "Poaceae", "Mustard", "Sinapis alba", "Phacelia tanacetifolia", "Trifolium alexandrinum", "", "biomass", "biomass", "biomass", "biomass", "biomass", "biomass", "Elements", "Nitrogen", "Nitrogen content", "Phosphorus", "Carbon", "Magnesium", "Potassium", "Boron", "Aluminium", "Manganese", "Sulphur", "Zinc", "Iron", "Copper", "Calcium", "Catch cropping", "Crop rotation", "cropping systems", "Biological competition", "Interspecific competition", "Shoot biomass", "catch crops", "mineral elements", "macro elements", "micro elements", "C/N ratio", "plant nutrition", "ICP-OES", "EA", "opendata", "Boden"], "contacts": [{"name": "Heuermann, Diana", "organization": "Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben", "position": "Staff member (Molecular Plant Nutrition)", "roles": ["author"], "phones": [{"value": "0049394825514"}], "emails": [{"value": "heuermannd@ipk-gatersleben.de"}], "addresses": [{"deliveryPoint": ["Correnstra\u00dfe 3"], "city": "Stadt Seeland", "administrativeArea": "Saxony-Anhalt", "postalCode": "06466", "country": "Germany"}], "links": [{"href": null}]}, {"name": "Wir\u00e9n, Nicolaus von", "organization": "Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben", "position": "Department head", "roles": ["projectLeader"], "phones": [{"value": "0049 39482 5603"}], "emails": [{"value": "vonwiren@ipk-gatersleben.de"}], "addresses": [{"deliveryPoint": ["Correnstra\u00dfe 3"], "city": "Stadt Seeland", "administrativeArea": "Saxony-Anhalt", "postalCode": "06466", "country": "Germany"}], "links": [{"href": null}]}, {"name": "BonaRes Data Centre", "organization": "Leibniz Centre for Agricultural Landscape Research (ZALF)", "position": "Research Platform 'Data' - WG Geodata", "roles": ["publisher"], "phones": [{"value": "+49 33432 82 171"}], "emails": [{"value": "bonares-datenzentrum@zalf.de"}], "addresses": [{"deliveryPoint": ["Eberswalder Strasse 84"], "city": "M\u00fcncheberg", "administrativeArea": "Brandenburg", "postalCode": "15374", "country": "Germany"}], "links": [{"href": null}]}, {"organization": "Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben", "roles": ["contributor"]}], "title_alternate": "Nutrient accumulation in the biomass of catch crop species in pure stands vs. mix at the beginning of a wheat-catch crop-maize long-term rotation"}, "links": [{"href": "https://maps.bonares.de/mapapps/resources/apps/bonares/index.html?lang=en&doi=8d34ddab-2bc9-4288-869b-a4afdd68f0dd", "rel": "download"}, {"rel": "self", "type": "application/geo+json", "title": "8d34ddab-2bc9-4288-869b-a4afdd68f0dd", "name": "item", "description": "8d34ddab-2bc9-4288-869b-a4afdd68f0dd", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/8d34ddab-2bc9-4288-869b-a4afdd68f0dd"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-21T00:00:00Z"}}, {"id": "PMC8544784", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:31:07Z", "type": "Journal Article", "created": "2021-10-11", "title": "Involvement of Arabidopsis Multi-Copper Oxidase-Encoding LACCASE12 in Root-to-Shoot Iron Partitioning: A Novel Example of Copper-Iron Crosstalk", "description": "
Numerous central biological processes depend on the participation of the essential elements iron (Fe) or copper (Cu), including photosynthesis, respiration, cell wall remodeling and oxidative stress protection. Yet, both Fe and Cu metal cations can become toxic when accumulated in excess. Because of the potent ligand-binding and redox chemistries of these metals, there is a need for the tight and combined homeostatic control of their uptake and distribution. Several known examples pinpoint an inter-dependence of Fe and Cu homeostasis in eukaryotes, mostly in green algae, yeast and mammals, but this is less well understood in multicellular plants to date. In Arabidopsis, Cu deficiency causes secondary Fe deficiency, and this is associated with reduced in vitro ferroxidase activity and decreased root-to-shoot Fe translocation. Here we summarize the current knowledge of the cross-talk between Cu and Fe homeostasis and present a partial characterization of LACCASE12 (LAC12) that encodes a member of the multicopper oxidase (MCO) protein family in Arabidopsis. LAC12 transcript levels increase under Fe deficiency. The phenotypic characterization of two mutants carrying T-DNA insertions suggests a role of LAC12 in root-to-shoot Fe partitioning and in maintaining growth on Fe-deficient substrates. A molecular understanding of the complex interactions between Fe and Cu will be important for combating Fe deficiency in crops and for advancing biofortification approaches.