{"type": "FeatureCollection", "features": [{"id": "10.1094/pdis-06-21-1276-pdn", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:17:33Z", "type": "Journal Article", "created": "2022-01-10", "title": "First Report of Multinucleate Rhizoctonia solani AG4 HG-I Causing Crown and Root Rot on Strawberry in Italy", "description": "Strawberry (Fragaria\u00a0\u00d7\u00a0ananassa\u00a0Duch.) is a crop of great economic importance in Italy, where it is grown in soil and under soilless conditions. In March 2019, about 30 to 35% of plants (cv. Portola) grown in a peat substrate under soilless conditions in a farm located in Cuneo Province died. The examination of 10 plants showed crown and root rot over 100% of the root/crown. Affected plants showed brown necrotic tissues in basal leaves and petiole necrosis. Crown and root tissues were cleaned thoroughly from soil residues under tap water. Portions (about 3 to 5 mm) from crowns and roots were cut and surface disinfected with a water solution of NaClO at 0.5% for 2 min and rinsed in sterile water. The tissue fragments were plated on potato dextrose agar (PDA) amended with 100 mg/liter of streptomycin sulfate and incubated at 25\u00b0C. After 3 days, fungal colonies with septate hyphae and right-angled branching similar to\u00a0Rhizoctonia solani\u00a0were observed with high frequency (90%) (Sneh et\u00a0al. 1991). To confirm the species identity, hyphal tips were transferred from the obtained colonies to PDA and grown for 10 days at 22 \u00b1 1\u00b0C. Mycelium was light brown, compact, with radial growth. The hyphal width varied from 8.5 to 10 \u03bcm. Sclerotia were not present. DNA was then extracted from a single representative isolate (RH230), and rDNA ITS sequencing was conducted as described by\u00a0Aiello et\u00a0al. (2017). The rDNA ITS sequence of RH230 (GenBank accession no. MZ373271) was 100% identical (603/603 bp) to part of another sequence previously identified as\u00a0R. solani\u00a0AG4 HG-I (MK583647,\u00a0Claerbout et\u00a0al. 2019). Twenty-day-old healthy plants of cultivar Portola were planted in a steam-disinfested peat soil (12-liter pots) infested with 1 g/liter of wheat kernels colonized for 10 days with the isolate RH230 to evaluate the pathogenicity. Control plants were planted in a steam-disinfested peat substrate amended with noninoculated sterilized wheat kernels. Six plants per treatments were used and kept in a greenhouse at 25 \u00b1 3\u00b0C. Crown and root rot similar to that observed in the farm developed 40 to 55 days after inoculation and resulted in 50 to 66% dead plants during two repeated trials. Fungal colonies morphologically similar to\u00a0R. solani\u00a0were consistently reisolated from affected crowns, and the resequencing of the rDNA ITS region fulfilled Koch\u2019s postulates. Control plants remained healthy.\u00a0Rhizoctonia\u00a0isolates of AG-A and AG-G anastomosis groups were found as pathogens of strawberry in Italy (Manici and Bonora 2007), while the AG4 HG-I was reported in Israel (Sharon et\u00a0al. 2007).\u00a0R. solani\u00a0AG4 HG-I was found on other hosts (Aiello et\u00a0al. 2017); however, to our knowledge, this is the first report on strawberry in Italy. The disease could become a significant problem for soilless culture strawberry in Italy, causing severe yield losses.", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Crown rot; Rhizoctonia; Root rot; Strawberry", "15. Life on land", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.1094/pdis-06-21-1276-pdn"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Disease", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1094/pdis-06-21-1276-pdn", "name": "item", "description": "10.1094/pdis-06-21-1276-pdn", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1094/pdis-06-21-1276-pdn"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}, {"id": "10.11591/.v3i4.4696", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:18:17Z", "type": "Journal Article", "created": "2019-11-04", "title": "Factors associated with Institutional delivery in Boricha district of Sidama zone, southern Ethiopia", "description": "

<p style='color: #000000; font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 10px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;'>Every year, 40 million women give birth at home without the help of a<br />skilled birth attendant. In 2011, 287,000 women died during pregnancy or childbirth. Almost all these deaths occur in developing countries where mothers and children lack access to basic health care. Reports showed the low utilization of health facility for delivery service in Ethiopia. This study aimed to determine the utilization and factors influencing institutional delivery. Community based cross sectional study was conducted from January to February 2013/14 in Boricha District of Southern Ethiopia among mothers who gave birth in the last 1 year. Multistage sampling techniques were used to collect data from 546 mothers. Taking in to account place of birth for the last child, only 4.9% women gave birth in a health facility. Women\u2019s education level (AOR=4.4 (95% CI=1.36-14.33)), timing of firstANC visit (AOR= .03 (95% CI=0.004 - 0.205)), women\u2019s advice to deliver in a health facility during ANC (AOR = 31.15 (95% CI=2.02-479.52)), women\u2019s knowledge of birth related complications (AOR= 12.4 (95% CI=2.67-57.16)) and decision making power (AOR=0.2 (95% CI=0.060.82)) showed significant association with institutionional delivery. Institutional delivery in the study area was found to be very low. Raising\u00a0awareness on institutional delivery to maximize delivery service utilization and strengthening provision of education and counseling to deliver in health facility during antenatal care visits at individual and community level should be given due emphasis.</p>

", "keywords": ["2. Zero hunger", "03 medical and health sciences", "0302 clinical medicine", "1. No poverty", "3. Good health"]}, "links": [{"href": "https://doi.org/10.11591/.v3i4.4696"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Public%20Health%20Science%20%28IJPHS%29", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.11591/.v3i4.4696", "name": "item", "description": "10.11591/.v3i4.4696", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.11591/.v3i4.4696"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-12-01T00:00:00Z"}}, {"id": "10.3389/fmicb.2019.02597", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:19:19Z", "type": "Journal Article", "created": "2019-11-08", "title": "New Insights Into Cinnamoyl Esterase Activity of Oenococcus oeni.", "description": "Some strains of Oenococcus oeni possess cinnamoyl esterase activity that can be relevant in the malolactic stage of wine production liberating hydroxycinnamic acids that are precursors of volatile phenols responsible for sensory faults. The objective of this study was to better understand the basis of the differential activity between strains. After initial screening, five commercial strains of O. oeni were selected, three were found to exhibit cinnamoyl esterase activity (CE+) and two not (CE-). Although the use of functional annotation of genes revealed genotypic variations between the strains, no specific genes common only to the three CE+ strains could explain the different activities. Pasteurized wine was used as a natural source of tartrate esters in growth and metabolism experiments conducted in MRS medium, whilst commercial trans-caftaric acid was used as substrate for enzyme assays. Detoxification did not seem to be the main biological mechanism involved in the activity since unlike its phenolic cleavage products and their immediate metabolites (trans-caffeic acid and 4-ethylcatechol), trans-caftaric acid was not toxic toward O. oeni. In the case of the two CE+ strains OenosTM and CiNeTM, wine-exposed samples showed a more rapid degradation of trans-caftaric acid than the unexposed ones. The CE activity was present in all cell-free extracts of both wine-exposed and unexposed strains, except in the cell-free extracts of the CE- strain CH11TM. This activity may be constitutive rather than induced by exposure to tartrate esters. Trans-caftaric acid was totally cleaved to trans-caffeic acid by cell-free extracts of the three CE+ strains, whilst cell-free extracts of the CE- strain CH16TM showed significantly lower activity, although higher for the strains in experiments with no prior wine exposure. The EstB28 esterase gene, found in the genomes of the 5 strains, did not reveal any difference on the upstream regulation and transport functionality between the strains. This study highlights the complexity of the basis of this activity in wine related O. oeni population. Variable cinnamoyl esterases or/and membrane transport activities in the O. oeni strains analyzed and a possible implication of wine molecules could explain this phenomenon.", "keywords": ["0301 basic medicine", "0303 health sciences", "tartrate esters", "cinnamoyl esterase", "Tartrate esters", "Hydroxycinnamic acids", "Wine", "hydroxycinnamic acids", "[SDV.IDA] Life Sciences [q-bio]/Food engineering", "Microbiology", "QR1-502", "03 medical and health sciences", "Cinnamoyl esterase", "wine", "Oenococcus oeni"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2019.02597"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2019.02597", "name": "item", "description": "10.3389/fmicb.2019.02597", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2019.02597"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-11-08T00:00:00Z"}}, {"id": "10.3389/fmicb.2022.983823", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:19:20Z", "type": "Journal Article", "created": "2022-11-08", "title": "Long-term effects of early-life rumen microbiota modulation on dairy cow production performance and methane emissions", "description": "

Rumen microbiota modulation during the pre-weaning period has been suggested as means to affect animal performance later in life. In this follow-up study, we examined the post-weaning rumen microbiota development differences in monozygotic twin-heifers that were inoculated (T-group) or not inoculated (C-group) (n\uffe2\uff80\uff89=\uffe2\uff80\uff894 each) with fresh adult rumen liquid during their pre-weaning period. We also assessed the treatment effect on production parameters and methane emissions of cows during their 1st lactation period. The rumen microbiota was determined by the 16S rRNA gene, 18S rRNA gene, and ITS1 amplicon sequencing. Animal weight gain and rumen fermentation parameters were monitored from 2 to 12\uffe2\uff80\uff89months of age. The weight gain was not affected by treatment, but butyrate proportion was higher in T-group in month 3 (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.04). Apart from archaea (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.084), the richness of bacteria (p\uffe2\uff80\uff89&lt;\uffe2\uff80\uff890.0001) and ciliate protozoa increased until month 7 (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.004) and anaerobic fungi until month 11 (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.005). The microbiota structure, measured as Bray\uffe2\uff80\uff93Curtis distances, continued to develop until months 3, 6, 7, and 10, in archaea, ciliate protozoa, bacteria, and anaerobic fungi, respectively (for all: p\uffe2\uff80\uff89=\uffe2\uff80\uff890.001). Treatment or age \uffc3\uff97 treatment interaction had a significant (p\uffe2\uff80\uff89&lt;\uffe2\uff80\uff890.05) effect on 18 bacterial, 2 archaeal, and 6 ciliate protozoan taxonomic groups, with differences occurring mostly before month 4 in bacteria, and month 3 in archaea and ciliate protozoa. Treatment stimulated earlier maturation of prokaryote community in T-group before month 4 and earlier maturation of ciliate protozoa at month 2 (Random Forest: 0.75\uffe2\uff80\uff89month for bacteria and 1.5\uffe2\uff80\uff89month for protozoa). No treatment effect on the maturity of anaerobic fungi was observed. The milk production and quality, feed efficiency, and methane emissions were monitored during cow\uffe2\uff80\uff99s 1st lactation. The T-group had lower variation in energy-corrected milk yield (p\uffe2\uff80\uff89&lt;\uffe2\uff80\uff890.001), tended to differ in pattern of residual energy intake over time (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.069), and had numerically lower somatic cell count throughout their 1st lactation period (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.081), but no differences between the groups in methane emissions (g/d, g/kg DMI, or g/kg milk) were observed. Our results demonstrated that the orally administered microbial inoculant induced transient changes in early rumen microbiome maturation. In addition, the treatment may influence the later production performance, although the mechanisms that mediate these effects need to be further explored.

", "keywords": ["microbiome modulation", "0301 basic medicine", "570", "ta412", "microbiome establishment", "Heifer", "dairy cow", "Rumen function", "Animal science", " dairy science", "Microbiology", "630", "Microbiome modulation", "QR1-502", "rumen function", "Microbiome establishment", "03 medical and health sciences", "Dairy cow", "heifer"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2022.983823"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2022.983823", "name": "item", "description": "10.3389/fmicb.2022.983823", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2022.983823"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-11-08T00:00:00Z"}}, {"id": "10.3390/plants9050583", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:19:31Z", "type": "Journal Article", "created": "2020-05-05", "title": "Response to Anthracnose in a Tarwi (Lupinus mutabilis) Collection Is Influenced by Anthocyanin Pigmentation", "description": "

Anthracnose, caused by Colletotrichum lupini, is a major limiting factor for lupin production. Tarwi or Andean Lupin (Lupinus mutabilis) is generally regarded as susceptible to anthracnose, but the high protein and oil content of its seeds raise interest in promoting its cultivation in Europe. In this study we evaluated the response to anthracnose of 10 tarwi accessions contrasting in anthocyanin pigmentation, by comparison to white lupin (Lupinus albus), using a contemporary Portuguese fungal isolate. A severity rating scale was optimized, including weighted parameters considering the type of symptoms and organs affected. All tarwi accessions were classified as susceptible, exhibiting sporulating necroses on the main stem from seven days after inoculation. Anthracnose severity was lower on anthocyanin-rich tarwi plants, with accession LM34 standing out as the less susceptible. Accession I82 better combines anthracnose response and yield. In global terms, disease severity was lower on white lupin than on tarwi. Although based on a limited collection, the results of the study show the existence of genetic variability among L. mutabilis towards anthracnose response relatable with anthocyanin pigmentation, providing insights for more detailed and thorough characterization of tarwi resistance to anthracnose.

", "keywords": ["0301 basic medicine", "Colletotrichum lupini", "Lupinus albus", "anthracnose", "0303 health sciences", "anthocyanin pigmentation", "Botany", "15. Life on land", "Lupinus mutabilis", "susceptibility", "Article", "03 medical and health sciences", "Lupinus albus", "13. Climate action", "QK1-989", "Lupinus mutabilis", "Colletotrichum lupini"]}, "links": [{"href": "http://www.mdpi.com/2223-7747/9/5/583/pdf"}, {"href": "https://www.mdpi.com/2223-7747/9/5/583/pdf"}, {"href": "https://doi.org/10.3390/plants9050583"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plants", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/plants9050583", "name": "item", "description": "10.3390/plants9050583", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/plants9050583"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-02T00:00:00Z"}}, {"id": "10.5194/bg-21-109-2024", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:19:58Z", "type": "Journal Article", "created": "2024-01-05", "title": "Geographically divergent trends in snow disappearance timing and fire ignitions across boreal North America", "description": "

Abstract. The snow cover extent across the Northern Hemisphere has diminished, while the number of lightning ignitions and amount of burned area have increased over the last 5\u00a0decades with accelerated warming. However, the effects of earlier snow disappearance on fire are largely unknown. Here, we assessed the influence of snow disappearance timing on fire ignitions across 16 ecoregions of boreal North America. We found spatially divergent trends in earlier (later) snow disappearance, which led to an increasing (decreasing) number of ignitions for the northwestern (southeastern) ecoregions between 1980 and 2019. Similar northwest\u2013southeast divergent trends were observed in the changing length of the snow-free season and correspondingly the fire season length. We observed increases (decreases) over northwestern (southeastern) boreal North America which coincided with a continental dipole in air temperature changes between 2001 and 2019. Earlier snow disappearance induced earlier ignitions of between 0.22 and 1.43\u2009d earlier per day of earlier snow disappearance in all ecoregions between 2001 and 2019. Early-season ignitions (defined by the 20\u2009% earliest fire ignitions per year) developed into significantly larger fires in 8 out of 16 ecoregions, being on average 77\u2009% larger across the whole domain. Using a piecewise structural equation model, we found that earlier snow disappearance is a good direct proxy for earlier ignitions but may also result in a cascade of effects from earlier desiccation of fuels and favorable weather conditions that lead to earlier ignitions. This indicates that snow disappearance timing is an important trigger of land\u2013atmosphere dynamics. Future warming and consequent changes in snow disappearance timing may contribute to further increases in western boreal fires, while it remains unclear how the number and timing of fire ignitions in eastern boreal North America may change with climate change.

", "keywords": ["0301 basic medicine", "QE1-996.5", "03 medical and health sciences", "Ecology", "Life", "13. Climate action", "QH501-531", "Geology", "15. Life on land", "01 natural sciences", "QH540-549.5", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://bg.copernicus.org/articles/21/109/2024/bg-21-109-2024.pdf"}, {"href": "https://doi.org/10.5194/bg-21-109-2024"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biogeosciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5194/bg-21-109-2024", "name": "item", "description": "10.5194/bg-21-109-2024", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5194/bg-21-109-2024"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-05T00:00:00Z"}}, {"id": "11245.1/e982467a-6b87-4f88-8ac3-53d0fb37aeb2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:22:24Z", "type": "Journal Article", "created": "2024-05-01", "title": "Arabinosylation of cell wall extensin is required for the directional response to salinity in roots", "description": "Abstract

Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses to salinity, we developed and exploited a real-time salt-induced tilting assay. This assay follows root growth upon both gravitropic and salt challenges, revealing that root bending upon tilting is modulated by Na+ ions, but not by osmotic stress. Next, we measured this salt-specific response in 345 natural Arabidopsis (Arabidopsis thaliana) accessions and discovered a genetic locus, encoding the cell wall-modifying enzyme EXTENSIN ARABINOSE DEFICIENT TRANSFERASE (ExAD) that is associated with root bending in the presence of NaCl (hereafter salt). Extensins are a class of structural cell wall glycoproteins known as hydroxyproline (Hyp)-rich glycoproteins, which are posttranslationally modified by O-glycosylation, mostly involving Hyp-arabinosylation. We show that salt-induced ExAD-dependent Hyp-arabinosylation influences root bending responses and cell wall thickness. Roots of exad1 mutant seedlings, which lack Hyp-arabinosylation of extensin, displayed increased thickness of root epidermal cell walls and greater cell wall porosity. They also showed altered gravitropic root bending in salt conditions and a reduced salt-avoidance response. Our results suggest that extensin modification via Hyp-arabinosylation is a unique salt-specific cellular process required for the directional response of roots exposed to salinity.Drylands (hyperarid, arid, semiarid, and dry subhumid ecosystems) cover almost half of Earth\uffe2\uff80\uff99s land surface and are highly vulnerable to environmental pressures. Here we provide an inventory of soil properties including carbon (C), nitrogen (N), and phosphorus (P) stocks within the current boundaries of drylands, aimed at serving as a benchmark in the face of future challenges including increased population, food security, desertification, and climate change. Aridity limits plant production and results in poorly developed soils, with coarse texture, low C:N and C:P, scarce organic matter, and high vulnerability to erosion. Dryland soils store 646 Pg of organic C to 2\uffe2\uff80\uff89m, the equivalent of 32% of the global soil organic C pool. The magnitude of the historic loss of C from dryland soils due to human land use and cover change and their typically low C:N and C:P suggest high potential to build up soil organic matter, but coarse soil textures may limit protection and stabilization processes. Restoring, preserving, and increasing soil organic matter in drylands may help slow down rising levels of atmospheric carbon dioxide by sequestering C, and is strongly needed to enhance food security and reduce the risk of land degradation and desertification.Soil moisture is among the most important factors regulating soil biodiversity and functioning. Models forecast changes in the precipitation regime in many areas of the planet, but how these changes will influence soil functioning, and how biotic drivers modulate such effects, is far from being understood. We evaluated the responses of C and N fluxes, and soil microbial properties to different soil water regimes in soils from the main three ecotypes of the world's largest and most diverse tropical savanna. Further, we explored the direct and indirect effects of changes in the ecotype and soil water regimes on these key soil processes. Soils from the woodland savanna showed a better nutritional status than the other ecotypes, as well as higher potential N cycling rates, N2O emissions, and soil bacterial abundance but lower bacterial richness, whereas potential CO2 emissions and CH4 uptake peaked in the intermediate savanna. The ecotype also modulated the effects of changes in the soil water regime on nutrient cycling, greenhouse gas fluxes, and soil bacterial properties, with more intense responses in the intermediate savanna. Further, we highlight the existence of multiple contrasting direct and indirect (via soil microbes and abiotic properties) effects of an intensification of the precipitation regime on soil C- and N-related processes. Our results confirm that ecotype is a fundamental driver of soil properties and functioning in the Cerrado and that it can determine the responses of key soil processes to changes in the soil water regime.Theory suggests that the diversity of molecules in soil organic matter (SOM functional diversity) provides key insights on multiple ecosystem services. We aimed to investigate how and why SOM functional diversity and composition change as topsoils develop, and its implications for key soil functions (e.g., from nutrient pool to water regulation).

Location

We reported data on 16 soil chronosequences globally distributed in nine countries from six continents.

Time Period

2016\uffe2\uff80\uff932017.

Major Taxa Studied

Soil microbes (bacteria and fungi) and vascular plants.

Methods

SOM functional diversity and composition without mineral interference were measured using diffuse reflectance mid\uffe2\uff80\uff90infrared Fourier transform spectroscopy (DRIFT). We aimed to characterize the main environmental factors related to SOM functional diversity and composition. Also, we calculated the links among SOM functional diversity and key soil functions.

Results

We found that SOM functional diversity declines after millions of years of soil formation (pedogenesis). We further showed that increases in plant cover and productivity led to a higher ratio of reduced (e.g., alkanes) over oxidized carbon forms (i.e., C: O\uffe2\uff80\uff90functional groups ratio), which was positively correlated to SOM functional diversity as soils age. Our findings indicated that the plant footprint (i.e., the accumulation of plant\uffe2\uff80\uff90derived material promoting the C: O\uffe2\uff80\uff90functional group ratio) would explain the reduction of SOM functional diversity as ecosystems develop. Moreover, the dissimilarity in SOM composition consistently increased with soil age, with the soil development stage emerging as the main predictor of SOM dissimilarity across contrasting biomes.

Main Conclusions

Our global survey contextualized the natural history of SOM functional diversity and composition during long\uffe2\uff80\uff90term soil development. Together, we showed how plant footprint drives the losses of SOM functional diversity with increasing age, which might provide a novel mechanism to explain typically reported losses in ecosystem functions during ecosystem retrogression.Growing environmental concern sparks renewed interest in the sustainable production of (bio)materials that can replace oil\uffe2\uff80\uff90derived goods. Polyhydroxyalkanoates (PHAs) are isotactic polymers that play a critical role in the central metabolism of producer bacteria, as they act as dynamic reservoirs of carbon and reducing equivalents. PHAs continue to attract industrial attention as a starting point toward renewable, biodegradable, biocompatible, and versatile thermoplastic and elastomeric materials. Pseudomonas species have been known for long as efficient biopolymer producers, especially for medium\uffe2\uff80\uff90chain\uffe2\uff80\uff90length PHAs. The surge of synthetic biology and metabolic engineering approaches in recent years offers the possibility of exploiting the untapped potential of Pseudomonas cell factories for the production of tailored PHAs. In this article, an overview of the metabolic and regulatory circuits that rule PHA accumulation in Pseudomonas putida is provided, and approaches leading to the biosynthesis of novel polymers (e.g., PHAs including nonbiological chemical elements in their structures) are discussed. The potential of novel PHAs to disrupt existing and future market segments is closer to realization than ever before. The review is concluded by pinpointing challenges that currently hinder the wide adoption of bio\uffe2\uff80\uff90based PHAs, and strategies toward programmable polymer biosynthesis from alternative substrates in engineered P. putida strains are proposed.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Biopolymer", "PHA", "Pseudomonas putida", "Polyhydroxyalkanoates", "Carbon", "12. Responsible consumption", "03 medical and health sciences", "/dk/atira/pure/sustainabledevelopmentgoals/affordable_and_clean_energy; name=SDG 7 - Affordable and Clean Energy", "Metabolic Engineering", "Pseudomonas", "Pathway engineering", "Metabolic engineering", "Synthetic biology"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.202000165"}, {"href": "https://doi.org/10.1002/biot.202000165"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biotechnology%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/biot.202000165", "name": "item", "description": "10.1002/biot.202000165", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/biot.202000165"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-11-09T00:00:00Z"}}, {"id": "10.1016/j.ijpddr.2024.100546", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:16:03Z", "type": "Journal Article", "created": "2024-05-05", "title": "Robenidine derivatives as potential antischistosomal drug candidates", "description": "Schistosomiasis caused by Schistosoma spp. is a disease that causes a considerable health burden to millions of people worldwide. The limited availability of effective drugs on the market and the increased risk of resistance development due to extensive usage, highlight the urgent need for new antischistosomal drugs. Recent studies have shown that robenidine derivatives, containing an aminoguanidine core, exhibit promising activities against Plasmodium falciparum, motivating further investigation into their efficacy against Schistosoma mansoni, due to their similar habitat and the resulting related cellular mechanisms like the heme detoxification pathway. The conducted phenotypic screening of robenidine and 80 derivatives against newly transformed schistosomula and adult Schistosoma mansoni yielded 11 candidates with low EC50 values for newly transformed schistosomula (1.12-4.63\u00a0\u03bcM) and adults (2.78-9.47\u00a0\u03bcM). The structure-activity relationship revealed that electron-withdrawing groups at the phenyl moiety, as well as the presence of methyl groups adjacent to the guanidine moiety, enhanced the activity of derivatives against both stages of Schistosoma mansoni. The two compounds 2,2'-Bis[(3-cyano-4-fluorophenyl)methylene] carbonimidic Dihydrazide Hydrochloride (1) and 2,2'-Bis[(4-difluoromethoxyphenyl) ethylidene] carbonimidic Dihydrazide Hydrochloride (19), were selected for an in vivo study in Schistosoma mansoni-infected mice based on their potency, cytotoxicity, pharmacokinetic-, and physicochemical properties, but failed to reduce the worm burden significantly (worm burden reduction <20%). Thus, robenidine derivatives require further refinements to obtain higher antischistosomal specificity and in vivo activity.", "keywords": ["Male", "0301 basic medicine", "0303 health sciences", "Drug discovery", "Infectious and parasitic diseases", "RC109-216", "Schistosoma mansoni", "Structure-activity relationship", "Guanidines", "Article", "Schistosomiasis mansoni", "3. Good health", "Mice", "Schistosomicides", "Structure-Activity Relationship", "03 medical and health sciences", "Aminoguanidine", "Animals", "Female", "Robenidine derivative"], "contacts": [{"organization": "Christian N. Lotz, Alina Krollenbrock, Lea Imhof, Michael Riscoe, Jennifer Keiser,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.ijpddr.2024.100546"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20for%20Parasitology%3A%20Drugs%20and%20Drug%20Resistance", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijpddr.2024.100546", "name": "item", "description": "10.1016/j.ijpddr.2024.100546", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijpddr.2024.100546"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-01T00:00:00Z"}}, {"id": "10.1002/ecy.1513", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:27Z", "type": "Journal Article", "created": "2016-07-02", "title": "Land Use Intensification In The Humid Tropics Increased Both Alpha And Beta Diversity Of Soil Bacteria", "description": "Abstract

Anthropogenic pressures on tropical forests are rapidly intensifying, but our understanding of their implications for biological diversity is still very limited, especially with regard to soil biota, and in particular soil bacterial communities. Here we evaluated bacterial community composition and diversity across a gradient of land use intensity in the eastern Amazon from undisturbed primary forest, through primary forests varyingly disturbed by fire, regenerating secondary forest, pasture, and mechanized agriculture. Soil bacteria were assessed by paired\uffe2\uff80\uff90end Illumina sequencing of 16S rRNA gene fragments (V4 region). The resulting sequences were clustered into operational taxonomic units (OTU) at a 97% similarity threshold. Land use intensification increased the observed bacterial diversity (both OTU richness and community heterogeneity across space) and this effect was strongly associated with changes in soil pH. Moreover, land use intensification and subsequent changes in soil fertility, especially pH, altered the bacterial community composition, with pastures and areas of mechanized agriculture displaying the most contrasting communities in relation to undisturbed primary forest. Together, these results indicate that tropical forest conversion impacts soil bacteria not through loss of diversity, as previously thought, but mainly by imposing marked shifts on bacterial community composition, with unknown yet potentially important implications for ecological functions and services performed by these communities.

", "keywords": ["Rios de composi\u00e7\u00e3o de comunidade bacteriana", "2. Zero hunger", "0301 basic medicine", "570", "0303 health sciences", "550", "Bacteria", "Biodiversidade subterr\u00e2nea", "Agriculture", "Biodiversity", "Forests", "15. Life on land", "Below\u2010ground biodiversity", "High\u2010throughput sequencing", "Soil", "03 medical and health sciences", "RNA", " Ribosomal", " 16S", "Sequenciamento de alto rendimento", "Rivers of bacterial community composition", "Soil Microbiology"]}, "links": [{"href": "https://eprints.lancs.ac.uk/id/eprint/82660/1/de_Carvalho_et_al_2016_raw_pdf.pdf"}, {"href": "https://doi.org/10.1002/ecy.1513"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ecy.1513", "name": "item", "description": "10.1002/ecy.1513", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ecy.1513"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-09T00:00:00Z"}}, {"id": "10.1016/j.jare.2020.02.015", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:16:04Z", "type": "Journal Article", "created": "2020-02-26", "title": "From winery waste to bioactive compounds and new polymeric biocomposites: A contribution to the circular economy concept", "description": "The paper aims at optimising and validating possible routes toward the full valorisation of grape agrowaste to produce bioactive molecules and new materials. Starting from Merlot red pomace, phenol complex mixtures were successfully extracted by using two different approaches. Extracts obtained by solvent-based (SE) technique contained up to 46.9 gGAeq/kgDW of total phenols. Depending on the used solvent, the prevalence of compounds belonging to different phenol families was achieved. Pressurized liquid extraction (PLE) gave higher total phenol yields (up to 79 gGAeq/kgDW) but a lower range of extracted compounds. All liquid extracts exerted strong antioxidant properties. Moreover, both SE and PLE extraction solid residues were directly exploited (between 5 and 20% w/w) to prepare biocomposite materials by direct mixing via an eco-friendly approach with PHBV polymer. The final composites showed mechanical characteristics similar to PHVB matrix. The use of pomace residues in biocomposites could therefore bring both to the reduction of the cost of the final material, as a lower amount of costly PHBV is used. The present research demonstrated the full valorisation of grape pomace, an agrowaste produced every year in large amounts and having a significant environmental impact.", "keywords": ["Medicine (General)", "Biocomposites", "Science (General)", "Agricultural Sciences", "Polyphenols", "Solvent-based extraction", "Biowaste", "04 agricultural and veterinary sciences", "Agriculture Forestry and Fisheries", "Grape pomace", "Biocomposites; Biowaste; Grape pomace; Polyphenols; Solvent-based extraction", " Pressurized liquid extraction", "01 natural sciences", "Article", "12. Responsible consumption", "Q1-390", "R5-920", "0404 agricultural biotechnology", "Pressurized liquid extraction", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://cris.unibo.it/bitstream/11585/758289/1/Ferri%20et%20al%202020%20JARE%20.pdf"}, {"href": "https://doi.org/10.1016/j.jare.2020.02.015"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Advanced%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jare.2020.02.015", "name": "item", "description": "10.1016/j.jare.2020.02.015", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jare.2020.02.015"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-07-01T00:00:00Z"}}, {"id": "10.1007/s00114-021-01748-8", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:39Z", "type": "Journal Article", "created": "2021-09-07", "title": "Societal importance of Antarctic negative feedbacks on climate change: blue carbon gains from sea ice, ice shelf and glacier losses", "description": "Abstract

Diminishing prospects for environmental preservation under climate change are intensifying efforts to boost capture, storage and sequestration (long-term burial) of carbon. However, as Earth\uffe2\uff80\uff99s biological carbon sinks also shrink, remediation has become a key part of the narrative for terrestrial ecosystems. In contrast, blue carbon on polar continental shelves have stronger pathways to sequestration and have increased with climate-forced marine ice losses\uffe2\uff80\uff94becoming the largest known natural negative feedback on climate change. Here we explore the size and complex dynamics of blue carbon gains with spatiotemporal changes in sea ice (60\uffe2\uff80\uff93100 MtCyear\uffe2\uff88\uff921), ice shelves (4\uffe2\uff80\uff9340 MtCyear\uffe2\uff88\uff921\uffe2\uff80\uff89=\uffe2\uff80\uff89giant iceberg generation) and glacier retreat (<\uffe2\uff80\uff891 MtCyear\uffe2\uff88\uff921). Estimates suggest that, amongst these, reduced duration of seasonal sea ice is most important. Decreasing sea ice extent drives longer (not necessarily larger biomass) smaller cell-sized phytoplankton blooms, increasing growth of many primary consumers and benthic carbon storage\uffe2\uff80\uff94where sequestration chances are maximal. However, sea ice losses also create positive feedbacks in shallow waters through increased iceberg movement and scouring of benthos. Unlike loss of sea ice, which enhances existing sinks, ice shelf losses generate brand new carbon sinks both where giant icebergs were, and in their wake. These also generate small positive feedbacks from scouring, minimised by repeat scouring at biodiversity hotspots. Blue carbon change from glacier retreat has been least well quantified, and although emerging fjords are small areas, they have high storage-sequestration conversion efficiencies, whilst blue carbon in polar waters faces many diverse and complex stressors. The identity of these are known (e.g. fishing, warming, ocean acidification, non-indigenous species and plastic pollution) but not their magnitude of impact. In order to mediate multiple stressors, research should focus on wider verification of blue carbon gains, projecting future change, and the broader environmental and economic benefits to safeguard blue carbon ecosystems through law.

", "keywords": ["0301 basic medicine", "0303 health sciences", "Blue carbon", "Ecologie", "Climate Change", "Sea ice", "Nature-based solutions", "Antarctic Regions", "Review", "Evolution des esp\u00e8ces", "Hydrogen-Ion Concentration", "15. Life on land", "7. Clean energy", "Carbon", "Feedback", "03 medical and health sciences", "13. Climate action", "Blue carbon \u00b7 Ecosystem services \u00b7 Sea ice \u00b7 Nature-based solutions \u00b7 Southern Ocean", "Ecosystem services", "Ice Cover", "Seawater", "14. Life underwater", "Southern Ocean", "Ecosystem"]}, "links": [{"href": "https://link.springer.com/content/pdf/10.1007/s00114-021-01748-8.pdf"}, {"href": "https://dipot.ulb.ac.be/dspace/bitstream/2013/332392/3/Barnes2021_Article_SocietalImportanceOfAntarcticN.pdf"}, {"href": "https://doi.org/10.1007/s00114-021-01748-8"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20Science%20of%20Nature", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00114-021-01748-8", "name": "item", "description": "10.1007/s00114-021-01748-8", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00114-021-01748-8"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-09-07T00:00:00Z"}}, {"id": "10.1007/s00122-021-03815-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:39Z", "type": "Journal Article", "created": "2021-03-25", "title": "Genomic prediction models trained with historical records enable populating the German ex situ genebank bio-digital resource center of barley (Hordeum\u00a0sp.) with information on resistances to soilborne barley mosaic viruses", "description": "Abstract Key message

Genomic prediction with special weight of major genes is a valuable tool to populate bio-digital resource centers.

Abstract

Phenotypic information of crop genetic resources is a prerequisite for an informed selection that aims to broaden the genetic base of the elite breeding pools. We investigated the potential of genomic prediction based on historical screening data of plant responses against the Barley yellow mosaic viruses for populating the bio-digital resource center of barley. Our study includes dense marker data for 3838 accessions of winter barley, and historical screening data of 1751 accessions for Barley yellow mosaic virus (BaYMV) and of 1771 accessions for Barley mild mosaic virus (BaMMV). Linear mixed models were fitted by considering combinations for the effects of genotypes, years, and locations. The best linear unbiased estimations displayed a broad spectrum of plant responses against BaYMV and BaMMV. Prediction abilities, computed as correlations between predictions and observed phenotypes of accessions, were low for the marker-assisted selection approach amounting to 0.42. In contrast, prediction abilities of genomic best linear unbiased predictions were high, with values of 0.62 for BaYMV and 0.64 for BaMMV. Prediction abilities of genomic prediction were improved by up to\uffe2\uff80\uff89~\uffe2\uff80\uff895% using W-BLUP, in which more weight is given to markers with significant major effects found by association mapping. Our results outline the utility of historical screening data and W-BLUP model to predict the performance of the non-phenotyped individuals in genebank collections. The presented strategy can be considered as part of the different approaches used in genebank genomics to valorize genetic resources for their usage in disease resistance breeding and research.

", "keywords": ["Genetic Markers", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Genotype", "Chromosome Mapping", "Genetic Variation", "Hordeum", "Genomics", "Potyviridae", "Linkage Disequilibrium", "Plant Breeding", "03 medical and health sciences", "Phenotype", "Databases", " Genetic", "Original Article", "Genetic Association Studies", "Disease Resistance", "Plant Diseases"]}, "links": [{"href": "https://link.springer.com/content/pdf/10.1007/s00122-021-03815-0.pdf"}, {"href": "https://doi.org/10.1007/s00122-021-03815-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Theoretical%20and%20Applied%20Genetics", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00122-021-03815-0", "name": "item", "description": "10.1007/s00122-021-03815-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00122-021-03815-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-03-25T00:00:00Z"}}, {"id": "10.1007/s13165-020-00330-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:15:14Z", "type": "Journal Article", "created": "2020-10-07", "title": "Current use of copper, mineral oils and sulphur for plant protection in organic horticultural crops across 10 European countries", "description": "The use of several plant protection inputs of mineral origin, such as copper, sulphur or mineral oils is seen as contentious by many consumers and stakeholders within the organic sector. Although the use of these inputs is legal in organic systems and also applied in non-organic agriculture, their use by organic growers raises questions for organic practice, which aspires to be free from toxic, non-renewable chemicals. Data on the current use of permitted plant protection inputs is currently scarce, especially in horticulture where chemical inputs deserve special attention since horticultural products are often readily edible. A mapping of the use of copper, sulphur and mineral oils was conducted by collecting expert knowledge across 10 European countries during May\u2013October 2018, i.e. before the limitation of copper use to 4\u00a0kg\u00a0ha\u22121\u00a0year\u22121 from February 1, 2019. Results show that copper is widely used by Mediterranean organic growers in citrus, olive, tomato and potato production. The annual limit of 6\u00a0kg\u00a0ha\u22121\u00a0year\u22121 was not always respected. We also found that tomato producers apply high amounts of copper in winter crops in greenhouses. Mineral oils are applied to control scales, mites and whiteflies. Sulphur is also commonly used by organic vegetable growers, especially in greenhouses. We conclude that the high usage found in various different crops (especially Mediterranean crops) confirms the need for researching alternatives.", "keywords": ["[SDV.SA]Life Sciences [q-bio]/Agricultural sciences", "0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "plant protection", "571", "Crop health", " quality", " protection", "Mediterranean crops", "Greenhouse crops", "tomato", "15. Life on land", "01 natural sciences", "[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy", "Organic-PLUSX", "03 medical and health sciences", "13. Climate action", "Contentious inputs", "Vegetables", "FiBL60073", "Mediterranean crops", " Greenhouse crops", " Tomato", " Contentious inputs", " plant protection", "Abacus"]}, "links": [{"href": "https://www.iris.unict.it/bitstream/20.500.11769/494877/1/Katsoulas2020_Article_CurrentUseOfCopperMineralOilsA%20%282%29.pdf"}, {"href": "https://link.springer.com/content/pdf/10.1007/s13165-020-00330-2.pdf"}, {"href": "https://doi.org/10.1007/s13165-020-00330-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Organic%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s13165-020-00330-2", "name": "item", "description": "10.1007/s13165-020-00330-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s13165-020-00330-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-10-07T00:00:00Z"}}, {"id": "10.1016/j.cej.2022.135748", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:15:38Z", "type": "Journal Article", "created": "2022-03-19", "title": "Evaluating bioelectrochemically-assisted constructed wetland (METland\u00ae) for treating wastewater: Analysis of materials, performance and electroactive communities", "description": "METland\u00ae technology consists of a bioengineering strategy for treating wastewater by integrating microbial electrochemical concepts into constructed wetland systems to enhance pollutants removal. In this context, we have constructed planted (Iris sibirica) biofilters to assess the impact of different electrically conductive bed materials (electroconductive coke, electroconductive biochar, non-electroconductive biochar and gravel) by analyzing the (i) wastewater treatment efficiency (COD and nitrogen removal), (ii) bioelectrochemical response, and (iii) diversity of microbial communities. Electrically conductive materials outperformed non-conductive ones allowing removal rates as high as 175-180 gCOD/bed*m3 day capable to support footprint as low 0.4 m2/pe. In contrast, the highest nitrogen removal rates were achieved with non-conductive biochar in presence of plants (80 %) regardless the anoxic conditions of the assay. This was confirmed by the presence of annamox bacteria like Planctomycetes. Furthermore, the presence of a marked electric potential profile along the bed height in electroconductive materials together with redox pairs (cyclic voltammetry analysis) demonstrated an effective electron flow from bottom to uppermost layers of the bed (geoconductor mechanism). In electroconductive biochar, such effective conductivity-based model co-exists with a geobattery mechanism due to presence of electroactive phenolic and carbonyl/quinone groups and/or microporosity. Microbial biodiversity analysis revealed the impact of plants just at the upper layers of the biofilters where roots and Rhizobium predominate. Bacteria from genus Clostridium were dominant in gravel inert material; in contrast, bacteria from genus Geobacter (12%) and Trichococcus (30%) outcompete the rest of communities for an effective colonization of carbonaceous beds, suggesting their main role as part of the electrosyntrophies mechanism after METland\u00ae.", "keywords": ["Electroactive bacteria", "0301 basic medicine", "0303 health sciences", "Metland\u00ae", "Electroconductive biochar", "Wastewater treatment", "QD Chemistry", "6. Clean water", "Constructed wetland", "03 medical and health sciences", "13. Climate action", "Microbial electrochemistry", "TD Environmental technology. Sanitary engineering", "Qu\u00edmica F\u00edsica"]}, "links": [{"href": "https://doi.org/10.1016/j.cej.2022.135748"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemical%20Engineering%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.cej.2022.135748", "name": "item", "description": "10.1016/j.cej.2022.135748", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.cej.2022.135748"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-07-01T00:00:00Z"}}, {"id": "10.1016/j.biocontrol.2022.104890", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:15:34Z", "type": "Journal Article", "created": "2022-03-14", "title": "Exploring microbiomes for plant disease management", "description": "Microbiome science is revolutionizing many concepts of plant biology, ecology, and evolution. Understanding plant microbiomes is key to developing solutions that protect crop health without impacting the environment. In this perspective article, we highlight the importance of both the structure and functions of plant-associated microbial communities in protecting their host from pathogens. These new findings have a high potential to aid biocontrol programs and to replace traditional chemical products, guiding the transition towards a sustainable production.", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "ddc:570", "15. Life on land", "Institut f\u00fcr Biochemie und Biologie"]}, "links": [{"href": "https://doi.org/10.1016/j.biocontrol.2022.104890"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biological%20Control", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biocontrol.2022.104890", "name": "item", "description": "10.1016/j.biocontrol.2022.104890", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biocontrol.2022.104890"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-06-01T00:00:00Z"}}, {"id": "10.1016/j.envint.2018.03.044", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:15:46Z", "type": "Journal Article", "created": "2018-04-06", "title": "Antibiotic resistance in wastewater treatment plants: Tackling the black box", "description": "Wastewater is among the most important reservoirs of antibiotic resistance in urban environments. The abundance of carbon sources and other nutrients, a variety of possible electron acceptors such as oxygen or nitrate, the presence of particles onto which bacteria can adsorb, or a fairly stable pH and temperature are examples of conditions favouring the remarkable diversity of microorganisms in this peculiar habitat. The wastewater microbiome brings together bacteria of environmental, human and animal origins, many harbouring antibiotic resistance genes (ARGs). Although numerous factors contribute, mostly in a complex interplay, for shaping this microbiome, the effect of specific potential selective pressures such as antimicrobial residues or metals, is supposedly determinant to dictate the fate of antibiotic resistant bacteria (ARB) and ARGs during wastewater treatment. This paper aims to enrich the discussion on the ecology of ARB&ARGs in urban wastewater treatment plants (UWTPs), intending to serve as a guide for wastewater engineers or other professionals, who may be interested in studying or optimizing the wastewater treatment for the removal of ARB&ARGs. Fitting this aim, the paper overviews and discusses: i) aspects of the complexity of the wastewater system and/or treatment that may affect the fate of ARB&ARGs; ii) methods that can be used to explore the resistome, meaning the whole ARB&ARGs, in wastewater habitats; and iii) some frequently asked questions for which are proposed addressing modes. The paper aims at contributing to explore how ARB&ARGs behave in UWTPs having in mind that each plant is a unique system that will probably need a specific procedure to maximize ARB&ARGs removal.", "keywords": ["0301 basic medicine", "Bacteria", "Microbiota", "SWOT analysis", "Wastewater", "15. Life on land", "Wastewater treatment optimization", "01 natural sciences", "6. Clean water", "Water Purification", "12. Responsible consumption", "03 medical and health sciences", "Anti-Infective Agents", "13. Climate action", "Drug Resistance", " Bacterial", "11. Sustainability", "Animals", "Humans", "Antibiotic resistance monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envint.2018.03.044"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environment%20International", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envint.2018.03.044", "name": "item", "description": "10.1016/j.envint.2018.03.044", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envint.2018.03.044"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-06-01T00:00:00Z"}}, {"id": "10.1016/j.geoderma.2023.116399", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:16:02Z", "type": "Journal Article", "created": "2023-02-27", "title": "One-time freeze-thawing or carbon input events have long-term legacies in soil microbial communities", "description": "Soil microbial communities are regularly exposed to sudden changes in environmental conditions, such as root exudation pulses or freeze-thaw events. As microbial communities have a high potential to adapt to changing conditions, they are expected to be resilient towards this kind of short-term perturbations and return to their pre-perturbed state quickly. Here, we conducted a lab incubation experiment to evaluate the resilience of soil microbial communities to single-pulse perturbations.

We incubated temperate forest soil at constant temperature (20 \u00b0C) and water content, and exposed it to strong single-pulse perturbations, which nonetheless mimic common pulse-events in temperate soils (glucose addition at 4 mg g\u22121 soil, or freeze-thawing overnight at \u221220 \u00b0C). We subsequently measured microbial community composition and microbial storage compounds via phospho- and neutral lipid fatty acid (PLFA and NLFA) profiling, as well as C/N stoichiometry of microbial biomass and dissolved organic carbon and nitrogen in the soil solution shortly after (0.4, 1, 4, and 6 days) and after longer time periods (84 and 160 days) following the perturbations.

Transferring the soils from their natural environment to the laboratory and incubating them under controlled conditions led to a continuous change of microbial community structure over time, along with an increase in microbial biomass and dissolved N in both perturbed and control soils over the time of the experiment. Against the background of this \u2018press-disturbance\u2019, caused by the permanently changed conditions, we see immediate and long-lasting effects of the single pulse events on microbial community composition, C storage and C/N stoichiometry. Both perturbations significantly influenced the microbial community structure (based on PLFA profiles), microbial biomass N and dissolved N up to 160 days, as well as fungal and bacterial biomass and storage (based on absolute PLFA and NLFA concentrations) up to 84 days. Both perturbations increased microbial N (+59.6 \u00b5g g\u22121 dw) and decreased dissolved N (\u221240.3 \u00b5g g\u22121 dw) after 160 days, and significantly altered C/N ratios in microbial and dissolved pools (particularly in the first 6 days of the experiment).

Our results demonstrate that single-pulse perturbations can have long-term legacies in soil microbial ecosystems. In our experiment they led to alternative system states which differed from the unperturbed control in multiple parameters even after 160 days. This indicates that soil microbial communities exhibit a low resistance and resilience towards single-pulse perturbations, and may easily be pushed on alternative trajectories by short but strong environmental pulses.", "keywords": ["0301 basic medicine", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "Resilience", "04 agricultural and veterinary sciences", "15. Life on land", "Perturbations", "6. Clean water", "Transient state", "Pulse event", "03 medical and health sciences", "106026 \u00d6kosystemforschung", "13. Climate action", "Soil microbial community", "106022 Microbiology", "0401 agriculture", " forestry", " and fisheries", "106026 Ecosystem research"]}, "links": [{"href": "https://doi.org/10.1016/j.geoderma.2023.116399"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Geoderma", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.geoderma.2023.116399", "name": "item", "description": "10.1016/j.geoderma.2023.116399", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.geoderma.2023.116399"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-04-01T00:00:00Z"}}, {"id": "10.1016/j.gca.2019.02.036", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:15:57Z", "type": "Journal Article", "created": "2019-02-27", "title": "Volatile distributions in and on the Moon revealed by Cu and Fe isotopes in the \u2018Rusty Rock\u2019 66095", "description": "Abstract The Apollo 16 \u2018Rusty Rock\u2019 impact melt breccia 66095 is a volatile-rich sample, with the volatiles inherited through vapor condensation from an internal lunar source formed during thermo-magmatic evolution of the Moon. We report Cu and Fe isotope data for 66095 and find that bulk-rocks, residues and acid leaches span a relatively limited range of compositions (3.0\u202f\u00b1\u202f1.3\u202fwt.% FeO [range\u202f=\u202f2.0\u20134.8\u202fwt.%], 5.4\u202f\u00b1\u202f3.1\u202fppm Cu [range\u202f=\u202f3\u201312\u202fppm], average \u03b456Fe of 0.15\u202f\u00b1\u202f0.05\u2030 [weighted mean\u202f=\u202f0.156\u2030] and \u03b465Cu of 0.72\u202f\u00b1\u202f0.14\u2030 [weighted mean\u202f=\u202f0.78\u2030]). In contrast to the extreme enrichment of the light isotopes of Zn and heavy isotopes of Cl in 66095, \u03b465Cu and \u03b456Fe in the sample lie within the previously reported range for lunar mare basalts (0.92\u202f\u00b1\u202f0.16\u2030 and 0.12\u202f\u00b1\u202f0.02\u2030, respectively). The lack of extreme isotopic fractionation for Cu and Fe isotopes reflects compositions inherent to 66095, with condensation of a cooling gas from impact-generated fumarolic activity at temperatures too low to lead to the condensation of Cu and Fe in the sample, but higher than required to condense Zn. Together with thermodynamic models, these constraints suggest that the gas condensed within 66095 between 700 and 900\u202f\u00b0C (assuming a pressure of 10\u22126 and an fO2 of IW-2). That the Cu and Fe isotopic compositions of sample 66095 are within the range of mare basalts removes the need for an exotic, volatile-enriched source. The enrichment in Tl, Br, Cd, Sn, Zn, Pb, Rb, Cs, Ga, B, Cl, Li relative to Bi, Se, Te, Ge, Cu, Ag, Sb, Mn, P, Cr and Fe in the \u2018Rusty Rock\u2019 is consistent with volcanic outgassing models and indicates that 66095 likely formed distal from the original source of the gas. The volatile-rich character of 66095 is consistent with impact-generated fumarolic activity in the region of the Cayley Plains, demonstrating that volatile-rich rocks can occur on the lunar surface from outgassing of a volatile-poor lunar interior. The \u2018Rusty Rock\u2019 indicates that the lunar interior is significantly depleted in volatile elements and compounds and that volatile-rich surface rocks likely formed through vapor condensation. Remote sensing studies have detected volatiles on the lunar surface, attributing them dominantly to solar wind. Based on the \u2018Rusty Rock\u2019, some of these surface volatiles may also originate from the Moon\u2019s interior.", "keywords": ["[SDU] Sciences of the Universe [physics]", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Condensates", "Copper isotopes", "13. Climate action", "Evaporation", "Iron isotopes", "Volatile elements", "Moon", "Rusty Rock"]}, "links": [{"href": "https://doi.org/10.1016/j.gca.2019.02.036"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Geochimica%20et%20Cosmochimica%20Acta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.gca.2019.02.036", "name": "item", "description": "10.1016/j.gca.2019.02.036", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.gca.2019.02.036"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-12-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2016.09.001", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:16:16Z", "type": "Journal Article", "created": "2016-09-09", "title": "Temperature Sensitivity Of Soil Respiration: Synthetic Effects Of Nitrogen And Phosphorus Fertilization On Chinese Loess Plateau", "description": "Nitrogen (N) and phosphorus (P) fertilization has the potential to alter soil respiration temperature sensitivity (Q10) by changing soil biochemical and crop physiological process. A four-year field experiment was conducted to determine how Q10 responded to these biochemical and physiological changes in rain-fed agro-ecosystems on the semi-arid Loess Plateau. Soil respiration, as well as biotic and abiotic factors were measured in winter wheat (Triticum aestivum L.), with three fertilization treatments: (no fertilization (CK), 160kgNhm-1 (N), and 160kgNha-1 with 39kgPha-1 (N+P). Mean annual soil respiration rate (calculated by averaging the four years) in the N treatment and N+P treatment was 18% and 48% higher than that in the CK treatment, respectively; and it was increased by 26% (14%-48%) in the N+P treatment as compared with that in the N treatment. The decrease of Q10 in the N and N+P treatments against the CK treatment was not stable for each year, ranging from 0.01 to 0.28. The maximum decrease of Q10 in the N and N+P treatments was 10% and 15% in 2014-2015, while in other years the decrease of Q10 was numerical but not significant. Soil microbial biomass carbon (SMBC) was increased by 10% and 50%, dissolved organic carbon (DOC) was increased by 6% and 21%, and photosynthesis rate was increased ranging from 6% to 33% with N and N+P fertilization. The relative abundance of Acidobacteria, Actinobacteria and Chloroflexi were significantly higher by 32.9%-54.1% in N addition soils (N and N+P) compared to CK treatment, whereas additional P application into soils increased the relative abundance of the family Micrococcaceae, Nocardioidaceae and Chitinophagaceae. Soil respiration was positively related to SMBC, DOC and photosynthesis rate (p<0.05). However, variation in Q10 may be related to the increase of soil mineral N content and variation of the relative abundance of soil microbial community in our study. Nitrogen and additional phosphorus fertilization regimes affect soil respiration and temperature sensitivity differently.", "keywords": ["0301 basic medicine", "2. Zero hunger", "03 medical and health sciences", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water"], "contacts": [{"organization": "Qingfang Liu, Rui Wang, Shengli Guo, Shengli Guo, Ying Wang, Qiqi Sun, Man Zhao, Yaxian Hu, Xin Gao, Lanlan Du,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2016.09.001"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2016.09.001", "name": "item", "description": "10.1016/j.scitotenv.2016.09.001", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2016.09.001"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-01T00:00:00Z"}}, {"id": "10.1371/journal.pone.0010867", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:18:25Z", "type": "Journal Article", "created": "2010-05-27", "title": "Ecosystem Carbon Stock Influenced by Plantation Practice: Implications for Planting Forests as a Measure of Climate Change Mitigation", "description": "Uncertainties remain in the potential of forest plantations to sequestrate carbon (C). We synthesized 86 experimental studies with paired-site design, using a meta-analysis approach, to quantify the differences in ecosystem C pools between plantations and their corresponding adjacent primary and secondary forests (natural forests). Totaled ecosystem C stock in plant and soil pools was 284 Mg C ha(-1) in natural forests and decreased by 28% in plantations. In comparison with natural forests, plantations decreased aboveground net primary production, litterfall, and rate of soil respiration by 11, 34, and 32%, respectively. Fine root biomass, soil C concentration, and soil microbial C concentration decreased respectively by 66, 32, and 29% in plantations relative to natural forests. Soil available N, P and K concentrations were lower by 22, 20 and 26%, respectively, in plantations than in natural forests. The general pattern of decreased ecosystem C pools did not change between two different groups in relation to various factors: stand age (< 25 years vs. > or = 25 years), stand types (broadleaved vs. coniferous and deciduous vs. evergreen), tree species origin (native vs. exotic) of plantations, land-use history (afforestation vs. reforestation) and site preparation for plantations (unburnt vs. burnt), and study regions (tropic vs. temperate). The pattern also held true across geographic regions. Our findings argued against the replacement of natural forests by the plantations as a measure of climate change mitigation.", "keywords": ["0106 biological sciences", "Conservation of Natural Resources", "Science", "Climate Change", "Ante-disciplinary", "01 natural sciences", "333", "Trees", "Inclusive", "Open Access", "Soil", "Engineering", "Interdisciplinary", "Biomass", "Biology", "PLOS", "Ecosystem", "2. Zero hunger", "Geography", "Research", "Physics", "Q", "Publications", "R", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Peer-review", "Carbon", "Open-Access", "Chemistry", "Public Library of Science", "13. Climate action", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0010867"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0010867", "name": "item", "description": "10.1371/journal.pone.0010867", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0010867"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-05-27T00:00:00Z"}}, {"id": "10.1371/journal.pone.0012346", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:18:25Z", "type": "Journal Article", "created": "2010-09-01", "title": "Fruit And Soil Quality Of Organic And Conventional Strawberry Agroecosystems", "description": "Sale of organic foods is one of the fastest growing market segments within the global food industry. People often buy organic food because they believe organic farms produce more nutritious and better tasting food from healthier soils. Here we tested if there are significant differences in fruit and soil quality from 13 pairs of commercial organic and conventional strawberry agroecosystems in California.At multiple sampling times for two years, we evaluated three varieties of strawberries for mineral elements, shelf life, phytochemical composition, and organoleptic properties. We also analyzed traditional soil properties and soil DNA using microarray technology. We found that the organic farms had strawberries with longer shelf life, greater dry matter, and higher antioxidant activity and concentrations of ascorbic acid and phenolic compounds, but lower concentrations of phosphorus and potassium. In one variety, sensory panels judged organic strawberries to be sweeter and have better flavor, overall acceptance, and appearance than their conventional counterparts. We also found the organically farmed soils to have more total carbon and nitrogen, greater microbial biomass and activity, and higher concentrations of micronutrients. Organically farmed soils also exhibited greater numbers of endemic genes and greater functional gene abundance and diversity for several biogeochemical processes, such as nitrogen fixation and pesticide degradation.Our findings show that the organic strawberry farms produced higher quality fruit and that their higher quality soils may have greater microbial functional capability and resilience to stress. These findings justify additional investigations aimed at detecting and quantifying such effects and their interactions.", "keywords": ["570", "Plant Extracts - analysis", "Nitrogen", "Science", "Agriculture - methods", "Fragaria - growth & development", "910", "Ante-disciplinary", "Soil - analysis", "Fragaria", "630", "Inclusive", "Carbon - analysis", "Open Access", "Soil", "Engineering", "Fruit - chemistry", "Interdisciplinary", "Biology", "PLOS", "2. Zero hunger", "Organic Agriculture", "Nitrogen - analysis", "Plant Extracts", "Research", "Physics", "Q", "R", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Fragaria - genetics", "Peer-review", "Carbon", "Open-Access", "Chemistry", "Public Library of Science", "Fruit - growth & development", "Fruit", "Fragaria - chemistry", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0012346"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0012346", "name": "item", "description": "10.1371/journal.pone.0012346", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0012346"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-09-01T00:00:00Z"}}, {"id": "10.1016/j.agrformet.2013.06.019", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:15:25Z", "type": "Journal Article", "created": "2013-08-03", "title": "Seasonal Dynamics Of Co2 Balance And Water Consumption Of C-3 And C-4-Type Cover Crops Compared To Bare Soil In A Suitability Study For Their Use In Vineyards In Germany And Argentina", "description": "Abstract Cover crops are used in vineyards to maintain soil structure, minimise soil erosion and optimise vineyard mechanisation, yet few data are available on water consumption and carbon dioxide assimilation (CO 2 ). Whereas cover crop use is common in the grape growing regions of Germany it is uncommon in Argentina. To obtain some information on the suitability of a selected number of species in terms of carbon gain and water expenditure under the climatic conditions of Germany and Argentina, we first quantified evapotranspiration (EvT), CO 2 assimilation and water use efficiency of Trifolium repens L. (white clover) (TR), Festuca arundinacea Schreb (tall fescue) (FE), Sorghum sudanense (Piper) Stapf (Sudan grass) (SO), and Digitaria californica (Benth) Henr. (Arizona cotton top) (DI) in a field trial in Geisenheim, Germany. We then compared the performance of TR, FE and DI together with Sorghum halepense L. (Johnson grass) (SH) in a pot experiment under the warmer climatic conditions of Mendoza, Argentina, where water supply could be controlled. In all cases bare soil served as a reference for soil water loss. Gas-exchange of plants and soil were measured with customised open system canopy chambers and an infrared gas analyser and water dynamics in the soil with soil moisture probes (field) or gravimetrically (pots). SO and TR were the two species that reached the highest degree of soil coverage in Germany and substantially reduced soil moisture in the first 0.40\u00a0m of depth. TR showed high EvT and CO 2 assimilation rates in both climates when water supply was sufficient and maintained relative high rates when water status was reduced under cool conditions in Germany, yet performed poorly when high temperatures occurred concomitant to low water supply in Argentina. DI, being a C 4 species from arid regions, had difficulties to get established in the cool conditions of Germany and there was no clear tendency for more efficient water use than the other cover crops. However under the warm condition of Argentina, especially when water supply was reduced, DI displayed higher WUE which was entirely related to the maintenance of higher assimilation rates at substantially reduced EvT. FE being common in German vineyards had low assimilation rates combined with high evapotranspiration rates under Argentinian conditions and was very sensitive to water deficit in combination with high temperature.", "keywords": ["0301 basic medicine", "2. Zero hunger", "03 medical and health sciences", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.1016/j.agrformet.2013.06.019"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agricultural%20and%20Forest%20Meteorology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agrformet.2013.06.019", "name": "item", "description": "10.1016/j.agrformet.2013.06.019", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agrformet.2013.06.019"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-11-01T00:00:00Z"}}, {"id": "PMC11101930", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:24:45Z", "type": "Journal Article", "created": "2024-05-05", "title": "Robenidine derivatives as potential antischistosomal drug candidates", "description": "Schistosomiasis caused by Schistosoma spp. is a disease that causes a considerable health burden to millions of people worldwide. The limited availability of effective drugs on the market and the increased risk of resistance development due to extensive usage, highlight the urgent need for new antischistosomal drugs. Recent studies have shown that robenidine derivatives, containing an aminoguanidine core, exhibit promising activities against Plasmodium falciparum, motivating further investigation into their efficacy against Schistosoma mansoni, due to their similar habitat and the resulting related cellular mechanisms like the heme detoxification pathway. The conducted phenotypic screening of robenidine and 80 derivatives against newly transformed schistosomula and adult Schistosoma mansoni yielded 11 candidates with low EC50 values for newly transformed schistosomula (1.12-4.63\u00a0\u03bcM) and adults (2.78-9.47\u00a0\u03bcM). The structure-activity relationship revealed that electron-withdrawing groups at the phenyl moiety, as well as the presence of methyl groups adjacent to the guanidine moiety, enhanced the activity of derivatives against both stages of Schistosoma mansoni. The two compounds 2,2'-Bis[(3-cyano-4-fluorophenyl)methylene] carbonimidic Dihydrazide Hydrochloride (1) and 2,2'-Bis[(4-difluoromethoxyphenyl) ethylidene] carbonimidic Dihydrazide Hydrochloride (19), were selected for an in vivo study in Schistosoma mansoni-infected mice based on their potency, cytotoxicity, pharmacokinetic-, and physicochemical properties, but failed to reduce the worm burden significantly (worm burden reduction <20%). Thus, robenidine derivatives require further refinements to obtain higher antischistosomal specificity and in vivo activity.", "keywords": ["Male", "0301 basic medicine", "0303 health sciences", "Drug discovery", "Infectious and parasitic diseases", "RC109-216", "Schistosoma mansoni", "Structure-activity relationship", "Guanidines", "Article", "Schistosomiasis mansoni", "3. Good health", "Mice", "Schistosomicides", "Structure-Activity Relationship", "03 medical and health sciences", "Aminoguanidine", "Animals", "Female", "Robenidine derivative"], "contacts": [{"organization": "Christian N. Lotz, Alina Krollenbrock, Lea Imhof, Michael Riscoe, Jennifer Keiser,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/PMC11101930"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20for%20Parasitology%3A%20Drugs%20and%20Drug%20Resistance", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC11101930", "name": "item", "description": "PMC11101930", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC11101930"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-01T00:00:00Z"}}, {"id": "PMC5507504", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:24:47Z", "type": "Journal Article", "created": "2017-07-13", "title": "Organic farming enhances soil microbial abundance and activity\u2014A meta-analysis and meta-regression", "description": "Population growth and climate change challenge our food and farming systems and provide arguments for an increased intensification of agriculture. A promising option is eco-functional intensification through organic farming, an approach based on using and enhancing internal natural resources and processes to secure and improve agricultural productivity, while minimizing negative environmental impacts. In this concept an active soil microbiota plays an important role for various soil based ecosystem services such as nutrient cycling, erosion control and pest and disease regulation. Several studies have reported a positive effect of organic farming on soil health and quality including microbial community traits. However, so far no systematic quantification of whether organic farming systems comprise larger and more active soil microbial communities compared to conventional farming systems was performed on a global scale. Therefore, we conducted a meta-analysis on current literature to quantify possible differences in key indicators for soil microbial abundance and activity in organic and conventional cropping systems. All together we integrated data from 56 mainly peer-reviewed papers into our analysis, including 149 pairwise comparisons originating from different climatic zones and experimental duration ranging from 3 to more than 100 years. Overall, we found that organic systems had 32% to 84% greater microbial biomass carbon, microbial biomass nitrogen, total phospholipid fatty-acids, and dehydrogenase, urease and protease activities than conventional systems. Exclusively the metabolic quotient as an indicator for stresses on microbial communities remained unaffected by the farming systems. Categorical subgroup analysis revealed that crop rotation, the inclusion of legumes in the crop rotation and organic inputs are important farming practices affecting soil microbial community size and activity. Furthermore, we show that differences in microbial size and activity between organic and conventional farming systems vary as a function of land use (arable, orchards, and grassland), plant life cycle (annual and perennial) and climatic zone. In summary, this study shows that overall organic farming enhances total microbial abundance and activity in agricultural soils on a global scale.", "keywords": ["Crops", " Agricultural", "2. Zero hunger", "Organic Agriculture", "Science", "Climate", "Microbiota", "Q", "R", "Fabaceae", "04 agricultural and veterinary sciences", "15. Life on land", "13. Climate action", "Life Science", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Soil Microbiology", "Research Article"]}, "links": [{"href": "https://doi.org/PMC5507504"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC5507504", "name": "item", "description": "PMC5507504", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC5507504"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-07-12T00:00:00Z"}}, {"id": "PMC9846495", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:24:51Z", "type": "Journal Article", "created": "2023-01-04", "title": "A genome wide association study to dissect the genetic architecture of agronomic traits in Andean lupin (Lupinus mutabilis)", "description": "

Establishing Lupinus mutabilis as a protein and oil crop requires improved varieties adapted to EU climates. The genetic regulation of strategic breeding traits, including plant architecture, growing cycle length and yield, is unknown. This study aimed to identify associations between 16 669 single nucleotide polymorphisms (SNPs) and 9 agronomic traits on a panel of 223 L. mutabilis accessions, grown in four environments, by applying a genome wide association study (GWAS). Seven environment-specific QTLs linked to vegetative yield, plant height, pods number and flowering time, were identified as major effect QTLs, being able to capture 6 to 20% of the phenotypic variation observed in these traits. Furthermore, two QTLs across environments were identified for flowering time on chromosome 8. The genes FAF, GAMYB and LNK, regulating major pathways involved in flowering and growth habit, as well as GA30X1, BIM1, Dr1, HDA15, HAT3, interacting with these pathways in response to hormonal and environmental cues, were prosed as candidate genes. These results are pivotal to accelerate the development of L. mutabilis varieties adapted to European cropping conditions by using marker-assisted selection (MAS), as well as to provide a framework for further functional studies on plant development and phenology in this species.Background: Water and nitrogen (N) are essential resources influencing plant growth and yield. To improve their efficiencies in crop production is challenging because the physiological mechanisms of water and N coupling and their interactive effect on crop water use efficiency (WUE) are not well understood yet.

Aim: The aim of this study was to investigate the physiological responses and phytohormones signaling in oats in response to soil water status and N supply under fertigation, to explore the mechanisms regulating plant growth and WUE.

Methods: Oat plants were subjected to the factorial combination of three soil moisture regimes (50, 70, and 90% of soil water holding capacity, SWHC) and three N levels (fertilized with 74, 149, and 298 mg kg\uffe2\uff88\uff921).

Results: The stomatal conductance (gs) was significantly decreased by soil water deficit, and also by the highest N level, whereas photosynthesis rate (An) was unaffected by neither water nor N. Consequently, intrinsic WUE (WUEint, An/gs) was highest under reduced irrigation and high N fertilization. This effect at stomatal level was affirmed by responses in whole plant WUE (WUEb), which was positively correlated with shoot \uffce\uffb413C. A positive correlation between \uffce\uffb418O and \uffce\uffb413C in shoots further indicated that decreases of gs rather than changes in An contributed to the enhanced WUE.

Conclusion: Moderate soil water deficit and sufficient N supply is recommended for saving irrigation water and improving WUE on fertigated oat plants without compromising biomass accumulation to any large extent.Broadband topological metamaterials hold the key for designing the next generation of integrated photonic platforms and microwave devices given their protected back\uffe2\uff80\uff90scattering\uffe2\uff80\uff90free and unidirectional edge states, among other exotic properties. However, synthesizing such metamaterial has proven challenging. Here, a broadband bandgap (relative bandwidth of more than 43%) Valley\uffe2\uff80\uff90Hall topological metamaterial with deep subwavelength thickness is proposed. The present topological metamaterial is composed of three layers printed circuit boards whose total thickness is 1.524\uffc2\uffa0mm \uffe2\uff89\uff88 \uffce\uffbb/100. The topological phase transition is achieved by introducing an asymmetry parameter \uffce\uffb4r. Three mechanically reconfigurable edge states can be obtained by varying interlayer displacement. Their robust transmission is demonstrated through two kinds of waveguide domain walls with cavities and disorders. Exploiting the proposed topological metamaterial, a six\uffe2\uff80\uff90way power divider is constructed and measured as a proof\uffe2\uff80\uff90of\uffe2\uff80\uff90concept of the potential of the proposed technology for future electromagnetic devices.Haloalkane dehalogenases (HLDs) are \uffce\uffb1/\uffce\uffb2\uffe2\uff80\uff90hydrolases that convert halogenated compounds to their corresponding alcohols. The overall kinetic mechanism proceeds via four steps: (i) binding of halogenated substrate, (ii) bimolecular nucleophilic substitution (SN2) leading to the cleavage of a carbon\uffe2\uff80\uff90halogen bond and the formation of an alkyl\uffe2\uff80\uff90enzyme intermediate, (iii) nucleophilic addition of a water molecule resulting in the hydrolysis of the intermediate to the corresponding alcohol and (iv) release of the reaction products \uffe2\uff80\uff93 an alcohol, a halide ion and a proton. Although, the overall reaction has been reported as irreversible, several kinetic evidences from previous studies suggest the reversibility of the first SN2 chemical step. To study this phenomenon, we have engineered HLDs to stop the catalytic cycle at the stage of the alkyl\uffe2\uff80\uff90enzyme intermediate. The ability of the intermediate to exchange halides was confirmed by a stopped\uffe2\uff80\uff90flow fluorescence binding analysis. Finally, the transhalogenation reaction was confirmed with several HLDs and 2,3\uffe2\uff80\uff90dichloropropene in the presence of a high concentration of iodide. The formation of the transhalogenation product 3\uffe2\uff80\uff90iodo\uffe2\uff80\uff902\uffe2\uff80\uff90chloropropene catalysed by five mutant HLDs was identified by gas chromatography coupled with mass spectrometry. Hereby we demonstrated the reversibility of the cleavage of the carbon\uffe2\uff80\uff90halogen bond by HLDs resulting in a transhalogenation. After optimization, the transhalogenation reaction can possibly find its use in biocatalytic applications. Enabling this reaction by strategically engineering the enzyme to stop at an intermediate in the catalytic cycle that is synthetically more useful than the product of the natural pathway is a novel concept.

magnified image

", "keywords": ["0301 basic medicine", "enzyme catalysis; halide exchange; haloalkane dehalogenase; nucleophilic substitution; redirection of reaction; transhalogenation", "0303 health sciences", "03 medical and health sciences"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/adsc.201900132"}, {"href": "https://doi.org/10.1002/adsc.201900132"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Advanced%20Synthesis%20%26amp%3B%20Catalysis", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/adsc.201900132", "name": "item", "description": "10.1002/adsc.201900132", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/adsc.201900132"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-17T00:00:00Z"}}, {"id": "10.1002/advs.201901408", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:25Z", "type": "Journal Article", "created": "2019-09-19", "title": "Natural Microbial Communities Can Be Manipulated by Artificially Constructed Biofilms", "description": "Abstract

Biofouling proceeds in successive steps where the primary colonizers affect the phylogenetic and functional structure of a future microbial consortium. Using microbiologically influenced corrosion (MIC) as a study case, a novel approach for material surface protection is described, which does not prevent biofouling, but rather shapes the process of natural biofilm development to exclude MIC\uffe2\uff80\uff90related microorganisms. This approach interferes with the early steps of natural biofilm formation affecting how the community is finally developed. It is based on a multilayer artificial biofilm, composed of electrostatically modified bacterial cells, producing antimicrobial compounds, extracellular antimicrobial polyelectrolyte matrix, and a water\uffe2\uff80\uff90proof rubber elastomer barrier. The artificial biofilm is constructed layer\uffe2\uff80\uff90by\uffe2\uff80\uff90layer (LBL) by manipulating the electrostatic interactions between microbial cells and material surfaces. Field testing on standard steel coupons exposed in the sea for more than 30 days followed by laboratory analyses using molecular\uffe2\uff80\uff90biology tools demonstrate that the preapplied artificial biofilm affects the phylogenetic structure of the developing natural biofilm, reducing phylogenetic diversity and excluding MIC\uffe2\uff80\uff90related bacteria. This sustainable solution for material protection showcases the usefulness of artificially guiding microbial evolutionary processes via the electrostatic modification and controlled delivery of bacterial cells and extracellular matrix to the exposed material surfaces.Gypsum soils in the Mediterranean Basin house large numbers of edaphic specialists that are adapted to stressful environments. The evolutionary history and standing genetic variation of these taxa have been influenced by the geological and paleoclimatic complexity of this area and the long\uffe2\uff80\uff90standing effect of human activities. However, little is known about the origin of Mediterranean gypsophiles and the factors affecting their genetic diversity and population structure.

METHODS

Using phylogenetic and phylogeographic approaches based on microsatellites and sequence data from nuclear and chloroplast regions, we evaluated the divergence time, genetic diversity, and population structure of 27 different populations of the widespread Iberian gypsophile Lepidium subulatum throughout its entire geographic range.

RESULTS

Lepidium subulatum diverged from its nearest relatives ~3 million years ago, and ITS and psbA/matK trees supported the monophyly of the species. These results suggest that both geological and climatic changes in the region around the Plio\uffe2\uff80\uff90Pleistocene promoted its origin, compared to other evolutionary processes. We found high genetic diversity in both nuclear and chloroplast markers, but a greater population structure in the chloroplast data. These results suggest that while seed dispersal is limited, pollen flow may be favored by the presence of numerous habitat patches that enhance the movement of pollinators.

CONCLUSIONS

Despite being an edaphic endemic, L. subulatum possesses high genetic diversity probably related to its relatively old age and high population sizes across its range. Our study highlights the value of using different markers to fully understand the phylogeographic history of plant species.There is an urgent need to develop viable, renewable, sustainable energy systems that can reduce global dependence on fossil fuel sources of energy. Biofuels such as ethanol are being utilized as blends in surface transportation fuels and have the potential to improve sustainability and reduce greenhouse gas emissions in the short term. Bioethanol, the most widely used liquid biofuel, is currently produced by converting sugars or starches from feed crops into ethanol. Use of this fuel source displaces and draws water consumption away from agricultural crops, increases soil erosion by shifting land from perennial grasses to annual crops, and increases use of fertilizers and insecticides. In contrast, bioethanol made from lignocellulosic biomass feedstocks does not have these limitations and in addition, offers a larger resource base: the amount of cellulosic material available for potential use vastly outweighs the amount of available starch\uffe2\uff80\uff90based feedstock. Therefore, bioethanol from lignocellulosic biomass has attracted considerable interest from biofuel developers. This review is an update of some developments to optimize cellulose extraction from feedstock crops and to improve crop yields and logistics. It concludes that agricultural and forestry systems that incorporate lignocellulosic biomass crops can be designed for improved ecological function and energy use efficiency. Development of crops that have both desirable cell\uffe2\uff80\uff90wall traits and high biomass productivity under sustainable low\uffe2\uff80\uff90input conditions can significantly enhance the economics and efficiency of the conversion process. Optimizing the logistics of moving feedstock from field or forest to bio\uffe2\uff80\uff90refinery can significantly reduce costs of using lignocellulosic feedstocks. \uffc2\uffa9 2013 Society of Chemical Industry and John Wiley & Sons, Ltd

", "keywords": ["0301 basic medicine", "2. Zero hunger", "03 medical and health sciences", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "7. Clean energy", "12. Responsible consumption"]}, "links": [{"href": "https://doi.org/10.1002/bbb.1418"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biofuels%2C%20Bioproducts%20and%20Biorefining", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/bbb.1418", "name": "item", "description": "10.1002/bbb.1418", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/bbb.1418"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-07-09T00:00:00Z"}}, {"id": "10.1002/bbb.325", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:25Z", "type": "Journal Article", "created": "2011-09-01", "title": "Biofuels: Ethics And Policy-Making", "description": "Abstract

Mandatory targets for biofuels have led to their rapid global adoption, but ethical problems with their large\uffe2\uff80\uff90scale production are widely reported. Research is underway to find new biofuel technologies that mitigate climate change and can be produced sustainably and economically. Following an 18\uffe2\uff80\uff90month inquiry, the Nuffield Council on Bioethics has developed an ethical framework for biofuels policy\uffe2\uff80\uff90making. Based on a number of widely held ethical values, six ethical principles are introduced that should be considered in biofuels policy\uffe2\uff80\uff90making. Many current biofuels policies fail this ethical \uffe2\uff80\uff98test\uffe2\uff80\uff99. An overarching ethical standard for biofuels is proposed that includes the protection of human rights and the environment, full life cycle assessment of greenhouse gas emissions, trade principles that are fair, access and benefit\uffe2\uff80\uff90sharing schemes to encourage equitable distribution of costs and benefits, and instruments to incentivize the development of promising new approaches. The Council goes on to make recommendations for changes to current policy. \uffc2\uffa9 2011 Society of Chemical Industry and John Wiley & Sons, Ltd

", "keywords": ["0301 basic medicine", "03 medical and health sciences", "13. Climate action", "01 natural sciences", "7. Clean energy", "0105 earth and related environmental sciences", "12. Responsible consumption", "3. Good health"], "contacts": [{"organization": "Joyce Tait, Alena Buyx,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1002/bbb.325"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biofuels%2C%20Bioproducts%20and%20Biorefining", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/bbb.325", "name": "item", "description": "10.1002/bbb.325", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/bbb.325"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-08-25T00:00:00Z"}}, {"id": "10.1002/cbic.202000051", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:25Z", "type": "Journal Article", "created": "2020-01-31", "title": "An Engineered E.\u2005coli Strain for Direct in Vivo Fluorination", "description": "Abstract

Selectively fluorinated compounds are found frequently in pharmaceutical and agrochemical products where currently 25\uffe2\uff80\uff9330\uffe2\uff80\uff89% of optimised compounds emerge from development containing at least one fluorine atom. There are many methods for the site\uffe2\uff80\uff90specific introduction of fluorine, but all are chemical and they often use environmentally challenging reagents. Biochemical processes for C\uffe2\uff88\uff92F bond formation are attractive, but they are extremely rare. In this work, the fluorinase enzyme, originally identified from the actinomycete bacterium Streptomyces cattleya, is engineered into Escherichia coli in such a manner that the organism is able to produce 5\uffe2\uff80\uffb2\uffe2\uff80\uff90fluorodeoxyadenosine (5\uffe2\uff80\uffb2\uffe2\uff80\uff90FDA) from S\uffe2\uff80\uff90adenosyl\uffe2\uff80\uff90l\uffe2\uff80\uff90methionine (SAM) and fluoride in live E.\uffe2\uff80\uff85coli cells. Success required the introduction of a SAM transporter and deletion of the endogenous fluoride efflux capacity in order to generate an E.\uffe2\uff80\uff85coli host that has the potential for future engineering of more elaborate fluorometabolites.

", "keywords": ["SAM transporters", "0301 basic medicine", "570", "S-Adenosylmethionine", "0303 health sciences", "Deoxyadenosines", "Halogenation", "DAS", "Fluorine", "Halogenations", "540", "QD Chemistry", "Streptomyces", "3. Good health", "03 medical and health sciences", "Bacterial Proteins", "Isomerism", "Escherichia coli", "QD", "Fluoride channels", "Genetic Engineering", "Oxidoreductases", "Fluorinases"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/cbic.202000051"}, {"href": "https://doi.org/10.1002/cbic.202000051"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ChemBioChem", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/cbic.202000051", "name": "item", "description": "10.1002/cbic.202000051", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/cbic.202000051"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-03T00:00:00Z"}}, {"id": "10.1002/ecy.2199", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:27Z", "type": "Journal Article", "created": "2018-02-27", "title": "Temperature and aridity regulate spatial variability of soil multifunctionality in drylands across the globe", "description": "Abstract

The relationship between the spatial variability of soil multifunctionality (i.e., the capacity of soils to conduct multiple functions; SVM) and major climatic drivers, such as temperature and aridity, has never been assessed globally in terrestrial ecosystems. We surveyed 236 dryland ecosystems from six continents to evaluate the relative importance of aridity and mean annual temperature, and of other abiotic (e.g., texture) and biotic (e.g., plant cover) variables as drivers of SVM, calculated as the averaged coefficient of variation for multiple soil variables linked to nutrient stocks and cycling. We found that increases in temperature and aridity were globally correlated to increases in SVM. Some of these climatic effects on SVM were direct, but others were indirectly driven through reductions in the number of vegetation patches and increases in soil sand content. The predictive capacity of our structural equation\uffc2\uffa0modelling was clearly higher for the spatial variability of N\uffe2\uff80\uff90 than for C\uffe2\uff80\uff90 and P\uffe2\uff80\uff90related soil variables. In the case of N cycling, the effects of temperature and aridity were both direct and indirect via changes in soil properties. For C and P, the effect of climate was mainly indirect via changes in plant attributes. These results suggest that future changes in climate may decouple the spatial availability of these elements for plants and microbes in dryland soils. Our findings significantly advance our understanding of the patterns and mechanisms driving SVM in drylands across the globe, which is critical for predicting changes in ecosystem functioning in response to climate change.Understanding anthropogenic influences on soil respiration (Rs) is critical for accurate predictions of soil carbon fluxes, but it is not known how Rs responds to grazing exclusion (GE). Here, we conducted a manipulative experiment in a meadow grassland on the Tibetan Plateau to investigate the effects of GE on Rs. The exclusion of livestock significantly increased soil moisture and above\uffe2\uff80\uff90ground biomass, but it decreased soil temperature, microbial biomass carbon (MBC), and Rs. Regression analysis indicated that the effects of GE on Rs were mainly due to changes in soil temperature, soil moisture, and MBC. Compared with the grazed blocks, GE significantly decreased soil carbon release by 23.6% over the growing season and 21.4% annually, but it increased the temperature sensitivity (Q10) of Rs by 6.5% and 14.2% for the growing season and annually respectively. Therefore, GE may reduce the release of soil carbon from the Tibetan Plateau, but under future climate warming scenarios, the increases in Q10 induced by GE could lead to increased carbon emissions.

", "keywords": ["570", "MICROBIAL RESPIRATION", "Environmental Sciences & Ecology", "Plant Productivity", "Temperature Sensitivity", "ALPINE GRASSLAND", "630", "Microbial Biomass Carbon", "NORTHERN CHINA", "SEASONAL PATTERNS", "MOUNTAIN GRASSLANDS", "Grazing Exclusion", "Tibetan Plateau", "PLANT-COMMUNITIES", "Original Research", "2. Zero hunger", "Science & Technology", "CLIMATE-CHANGE", "CO2 EFFLUX", "Ecology", "04 agricultural and veterinary sciences", "15. Life on land", "INNER-MONGOLIA", "BELOW-GROUND BIOMASS", "Soil Respiration", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Life Sciences & Biomedicine"]}, "links": [{"href": "https://doi.org/10.1002/ece3.1867"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.1867", "name": "item", "description": "10.1002/ece3.1867", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.1867"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-01-11T00:00:00Z"}}, {"id": "10.1002/ece3.1879", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:26Z", "type": "Journal Article", "created": "2016-01-22", "title": "Effects Of Fertilization On Microbial Abundance And Emissions Of Greenhouse Gases (Ch4 And N2o) In Rice Paddy Fields", "description": "Abstract

This study is to explore effects of nitrogen application and straw incorporation on abundance of relevant microbes and CH4 and N2O fluxes in a midseason aerated rice paddy field. Fluxes of CH4 and N2O were recorded, and abundance of relevant soil microbial functional genes was determined during rice\uffe2\uff80\uff90growing season in a 6\uffe2\uff80\uff90year\uffe2\uff80\uff90long fertilization experiment field in China. Results indicate that application of urea significantly changed the functional microbial composition, while the influence of straw incorporation was not significant. Application of urea significantly decreased the gene abundances of archaeal amoA and mcrA, but it significantly increased the gene abundances of bacterial amoA. CH4 emission was significantly increased by fresh straw incorporation. Incorporation of burnt straw tended to increase CH4 emission, while the urea application had no obvious effect on CH4 emission. N2O emission was significantly increased by urea application, while fresh or burnt straw incorporation tended to decrease N2O emission. The functional microbial composition did not change significantly over time, although the abundances of pmoA, archaeal amoA, nirS, and nosZ genes changed significantly. The change of CH4 emission showed an inverse trend with the one of the N2O emissions over time. To some extent, the abundance of some functional genes in this study can explain CH4 and N2O emissions. However, the correlation between CH4 and N2O emissions and the abundance of related functional genes was not significant. Environmental factors, such as soil Eh, may be more related to CH4 and N2O emissions.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "03 medical and health sciences", "13. Climate action", "15. Life on land", "Original Research"]}, "links": [{"href": "https://doi.org/10.1002/ece3.1879"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.1879", "name": "item", "description": "10.1002/ece3.1879", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.1879"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-01-22T00:00:00Z"}}, {"id": "10.1002/ece3.5244", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:26Z", "type": "Journal Article", "created": "2019-06-10", "title": "Evolution of interdisciplinarity in biodiversity science", "description": "Abstract

The study of biodiversity has grown exponentially in the last thirty years in response to demands for greater understanding of the function and importance of Earth's biodiversity and finding solutions to conserve it. Here, we test the hypothesis that biodiversity science has become more interdisciplinary over time. To do so, we analyze 97,945 peer\uffe2\uff80\uff90reviewed articles over a twenty\uffe2\uff80\uff90two\uffe2\uff80\uff90year time period (1990\uffe2\uff80\uff932012) with a continuous time dynamic model, which classifies articles into concepts (i.e., topics and ideas) based on word co\uffe2\uff80\uff90occurrences. Using the model output, we then quantify different aspects of interdisciplinarity: concept diversity, that is, the diversity of topics and ideas across subdisciplines in biodiversity science, subdiscipline diversity, that is, the diversity of subdisciplines across concepts, and network structure, which captures interactions between concepts and subdisciplines. We found that, on average, concept and subdiscipline diversity in biodiversity science were either stable or declining, patterns which were driven by the persistence of rare concepts and subdisciplines and a decline in the diversity of common concepts and subdisciplines, respectively. Moreover, our results provide evidence that conceptual homogenization, that is, decreases in temporal \uffce\uffb2 concept diversity, underlies the observed trends in interdisciplinarity. Together, our results reveal that biodiversity science is undergoing a dynamic phase as a scientific discipline that is consolidating around a core set of concepts. Our results suggest that progress toward addressing the biodiversity crisis via greater interdisciplinarity during the study period may have been slowed by extrinsic factors, such as the failure to invest in research spanning across concepts and disciplines. However, recent initiatives such as the Intergovernmental Science\uffe2\uff80\uff90Policy Platform on Biodiversity and Ecosystem Services (IPBES) may attract broader support for biodiversity\uffe2\uff80\uff90related issues and hence interdisciplinary approaches to address scientific, political, and societal challenges in the coming years.

", "keywords": ["0301 basic medicine", "bibliographic analysis", "topic models", "0303 health sciences", "03 medical and health sciences", "conceptual homogenization", "interdisciplinarity", "Ecology", "13. Climate action", "15. Life on land", "Academic Practice in Ecology and Evolution", "QH540-549.5", "biodiversity"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.5244"}, {"href": "https://doi.org/10.1002/ece3.5244"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.5244", "name": "item", "description": "10.1002/ece3.5244", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.5244"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-01T00:00:00Z"}}, {"id": "10.1002/ece3.6547", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:26Z", "type": "Journal Article", "created": "2020-07-09", "title": "The distribution of herbivores between leaves matches their performance only in the absence of competitors", "description": "Abstract

Few studies have tested how plant quality and the presence of competitors interact in determining how herbivores choose between different leaves within a plant. We investigated this in two herbivorous spider mites sharing tomato plants: Tetranychus urticae, which generally induces plant defenses, and Tetranychus evansi, which suppresses them, creating asymmetrical effects on coinfesting competitors. On uninfested plants, both herbivore species preferred young leaves, coinciding with increased mite performance. On plants with heterospecifics, the mites did not prefer leaves on which they had a better performance. In particular, T.\uffc2\uffa0urticae avoided leaves infested with T.\uffc2\uffa0evansi, which is in agreement with T.\uffc2\uffa0urticae being outcompeted by T.\uffc2\uffa0evansi. In contrast, T.\uffc2\uffa0evansi did not avoid leaves with the other species, but distributed itself evenly over plants infested with heterospecifics. We hypothesize that this behavior of T.\uffc2\uffa0evansi may prevent further spread of T.\uffc2\uffa0urticae over the shared plant. Our results indicate that leaf age determines within\uffe2\uff80\uff90plant distribution of herbivores only in absence of competitors. Moreover, they show that this distribution depends on the order of arrival of competitors and on their effects on each other, with herbivores showing differences in behavior within the plant as a possible response to the outcome of those interactions.Long amplicon metabarcoding has opened the door for phylogenetic analysis of the largely unknown communities of microeukaryotes in soil. Here, we amplified and sequenced the ITS and LSU regions of the rDNA operon (around 1500\uffc2\uffa0bp) from grassland soils using PacBio SMRT sequencing. We tested how three different methods for generation of operational taxonomic units (OTUs) effected estimated richness and identified taxa, and how well large\uffe2\uff80\uff90scale ecological patterns associated with shifting environmental conditions were recovered in data from the three methods. The field site at Kungs\uffc3\uffa4ngen Nature Reserve has drawn frequent visitors since Linnaeus's time, and its species rich vegetation includes the largest population of Fritillaria meleagris in Sweden. To test the effect of different OTU generation methods, we sampled soils across an abrupt moisture transition that divides the meadow community into a Carex acuta dominated plant community with low species richness in the wetter part, which is visually distinct from the mesic\uffe2\uff80\uff90dry part that has a species rich grass\uffe2\uff80\uff90dominated plant community including a high frequency of F.\uffc2\uffa0meleagris. We used the moisture and plant community transition as a framework to investigate how detected belowground microeukaryotic community composition was influenced by OTU generation methods. Soil communities in both moisture regimes were dominated by protists, a large fraction of which were taxonomically assigned to Ciliophora (Alveolata) while 30%\uffe2\uff80\uff9340% of all reads were assigned to kingdom Fungi. Ecological patterns were consistently recovered irrespective of OTU generation method used. However, different methods strongly affect richness estimates and the taxonomic and phylogenetic resolution of the characterized community with implications for how well members of the microeukaryotic communities can be recognized in the data.

", "keywords": ["580", "species hypothesis", "Ekologi", "0301 basic medicine", "0303 health sciences", "Ecology", "rDNA", "Biological Systematics", "15. Life on land", "03 medical and health sciences", "14. Life underwater", "ITS", "Research Articles", "clustering"]}, "links": [{"href": "https://pub.epsilon.slu.se/27699/1/eshghi-sahraei-s-et-al-220505.pdf"}, {"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.8676"}, {"href": "https://doi.org/10.1002/ece3.8676"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.8676", "name": "item", "description": "10.1002/ece3.8676", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.8676"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-03-01T00:00:00Z"}}, {"id": "10.1002/ecs2.2226", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:27Z", "type": "Journal Article", "created": "2018-05-31", "title": "Mycorrhiza in tree diversity-ecosystem function relationships: conceptual framework and experimental implementation", "description": "Abstract

The widely observed positive relationship between plant diversity and ecosystem functioning is thought to be substantially driven by complementary resource use of plant species. Recent work suggests that biotic interactions among plants and between plants and soil organisms drive key aspects of resource use complementarity. Here, we provide a conceptual framework for integrating positive biotic interactions across guilds of organisms, more specifically between plants and mycorrhizal types, to explain resource use complementarity in plants and its consequences for plant competition. Our overarching hypothesis is that ecosystem functioning increases when more plant species associate with functionally dissimilar mycorrhizal fungi because differing mycorrhizal types will increase coverage of habitat space for and reduce competition among plants. We introduce a recently established field experiment (MyDiv) that uses different pools of tree species that associate with either arbuscular or ectomycorrhizal fungi to create orthogonal experimental gradients in tree species richness and mycorrhizal associations and present initial results. Finally, we discuss options for future mechanistic studies on resource use complementarity within MyDiv. We show how mycorrhizal types and biotic interactions in MyDiv can be used in the future to test novel questions regarding the mechanisms underlying biodiversity\uffe2\uff80\uff93ecosystem function relationships.

", "keywords": ["0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "biodiversity\u2013ecosystem functioning", "experimental design", "Ecology", "arbuscular mycorrhiza", "15. Life on land", "01 natural sciences", "ectomycorrhiza", "Article", "biotic interactions", "03 medical and health sciences", "biodiversity effects", "QH540-549.5"]}, "links": [{"href": "https://esajournals.onlinelibrary.wiley.com/doi/pdf/10.1002/ecs2.2226"}, {"href": "https://doi.org/10.1002/ecs2.2226"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ecs2.2226", "name": "item", "description": "10.1002/ecs2.2226", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ecs2.2226"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-05-01T00:00:00Z"}}, {"id": "10.1002/ecs2.2645", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-26T16:14:27Z", "type": "Journal Article", "created": "2019-03-19", "title": "Uneven global distribution of food web studies under climate change", "description": "Abstract

Trophic interactions within food webs affect species distributions, coexistence, and provision of ecosystem services but can be strongly impacted by climatic changes. Understanding these impacts is therefore essential for managing ecosystems and sustaining human well\uffe2\uff80\uff90being. Here, we conducted a global synthesis of terrestrial, marine, and freshwater studies to identify key gaps in our knowledge of climate change impacts on food webs and determine whether the areas currently studied are those most likely to be impacted by climate change. We found research suffers from a strong geographic bias, with only 3.5% of studies occurring in the tropics. Importantly, the distribution of sites sampled under projected climate changes was biased\uffe2\uff80\uff94areas with decreases or large increases in precipitation and areas with low magnitudes of temperature change were under\uffe2\uff80\uff90represented. Our results suggest that understanding of climate change impacts on food webs could be broadened by considering more than two trophic levels, responses in addition to species abundance and biomass, impacts of a wider suite of climatic variables, and tropical ecosystems. Most importantly, to enable better forecasts of biodiversity responses to climate change, we identify critically under\uffe2\uff80\uff90represented geographic regions and climatic conditions which should be prioritized in future research.Changes in substrate quality driven by climate, land use, or other forms of global change may represent a strong selective force on microbial communities. Invasion of new taxa into a community through dispersal, evolution, or recolonization could impact the outcome of this environmental selection. Here, we simulated substrate change with a trait\uffe2\uff80\uff90based model of microbial litter decomposition (DEMENTpy) to assess the legacy effects of past substrate quality and the impact of selection by a new substrate on community decomposition activity. Simulations were run with different levels of invasion, including invasion from communities long\uffe2\uff80\uff90adapted to the new substrate. Legacy effects were evident with substrate change for native communities differing in composition. Protein was the only substrate that exerted a strong enough selective force to affect community composition. Legacy effects disappeared when invaders came from substrates similar to the new substrate. Together, our simulations demonstrate that substrate quality changes associated with global change can lead to legacy effects on substrate degradation. In decomposing plant litter, such legacy effects can occur if substrate inputs shift to higher protein content and if invasion is low.The ecological drivers of soil biodiversity in the Southern Hemisphere remain underexplored. Here, in a continental survey comprising 647 sites, across 58 degrees of latitude between tropical Australia and Antarctica, we evaluated the major ecological patterns in soil biodiversity and relative abundance of ecological clusters within a co\uffe2\uff80\uff90occurrence network of soil bacteria, archaea and eukaryotes. Six major ecological clusters (modules) of co\uffe2\uff80\uff90occurring soil taxa were identified. These clusters exhibited strong shifts in their relative abundances with increasing distance from the equator. Temperature was the major environmental driver of the relative abundance of ecological clusters when Australia and Antarctica are analyzed together. Temperature, aridity, soil properties and vegetation types were the major drivers of the relative abundance of different ecological clusters within Australia. Our data supports significant reductions in the diversity of bacteria, archaea and eukaryotes in Antarctica vs. Australia linked to strong reductions in temperature. However, we only detected small latitudinal variations in soil biodiversity within Australia. Different environmental drivers regulate the diversity of soil archaea (temperature and soil carbon), bacteria (aridity, vegetation attributes and pH) and eukaryotes (vegetation type and soil carbon) across Australia. Together, our findings provide new insights into the mechanisms driving soil biodiversity in the Southern Hemisphere.