{"type": "FeatureCollection", "features": [{"id": "10.1007/s10533-015-0157-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:15:20Z", "type": "Journal Article", "created": "2015-11-14", "title": "Chronic Nitrogen Fertilization And Carbon Sequestration In Grassland Soils: Evidence Of A Microbial Enzyme Link", "description": "Chronic nitrogen (N) fertilization can greatly affect soil carbon (C) sequestration by altering biochemical interactions between plant detritus and soil microbes. In lignin-rich forest soils, chronic N additions tend to increase soil C content partly by decreasing the activity of lignin-degrading enzymes. In cellulose-rich grassland soils it is not clear whether cellulose-degrading enzymes are also inhibited by N additions and what consequences this might have on changes in soil C content. Here we address whether chronic N fertilization has affected (1) the C content of light versus heavier soil fractions, and (2) the activity of four extracellular enzymes including the C-acquiring enzyme \u03b2-1,4-glucosidase (BG; necessary for cellulose hydrolysis). We found that 19\u00a0years of chronic N-only addition to permanent grassland have significantly increased soil C sequestration in heavy but not in light soil density fractions, and this C accrual was associated with a significant increase (and not decrease) of BG activity. Chronic N fertilization may increase BG activity because greater N availability reduces root C:N ratios thus increasing microbial demand for C, which is met by C inputs from enhanced root C pools in N-only fertilized soils. However, BG activity and total root mass strongly decreased in high pH soils under the application of lime (i.e. CaCO3), which reduced the ability of these organo-mineral soils to gain more C per units of N added. Our study is the first to show a potential \u2018enzyme link\u2019 between (1) long-term additions of inorganic N to grassland soils, and (2) the greater C content of organo-mineral soil fractions. Our new hypothesis is that the \u2018enzyme link\u2019 occurs because (a) BG activity is stimulated by increased microbial C demand relative to N under chronic fertilization, and (b) increased BG activity causes more C from roots and from microbial metabolites to accumulate and stabilize into organo-mineral C fractions. We suggest that any combination of management practices that can influence the BG \u2018enzyme link\u2019 will have far reaching implications for long-term C sequestration in grassland soils.", "keywords": ["DECOMPOSITION", "DYNAMICS", "570", "\u03b2-1", "4-Glucosidase", "/dk/atira/pure/subjectarea/asjc/2300/2304", "NUTRIENT RELEASE", "Environmental Sciences & Ecology", "Root C:N ratio", "Extracellular enzyme activity", "LITTER DECAY", "FOREST ECOSYSTEMS", "0399 Other Chemical Sciences", "0402 Geochemistry", "Environmental Chemistry", "Geosciences", " Multidisciplinary", "beta-1", "4-Glucosidase", "Earth-Surface Processes", "Water Science and Technology", "2. Zero hunger", "Multidisciplinary", "Science & Technology", "/dk/atira/pure/subjectarea/asjc/1900/1904", "Geology", "sequestration", "Agronomy & Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Soil carbon", "N DEPOSITION", "ORGANIC-MATTER", "PHOSPHORUS", "Fertilization", "Physical Sciences", "N ratio [Root C]", "0401 agriculture", " forestry", " and fisheries", "Soil carbon sequestration", "Liming", "TURNOVER", "Life Sciences & Biomedicine", "Geosciences", "/dk/atira/pure/subjectarea/asjc/2300/2312", "Environmental Sciences", "RESPONSES"]}, "links": [{"href": "https://doi.org/10.1007/s10533-015-0157-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biogeochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10533-015-0157-5", "name": "item", "description": "10.1007/s10533-015-0157-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10533-015-0157-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-11-14T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2004.00883.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:32Z", "type": "Journal Article", "created": "2004-12-02", "title": "Carbon Flow In An Upland Grassland: Effect Of Liming On The Flux Of Recently Photosynthesized Carbon To Rhizosphere Soil", "description": "Abstract<p>The effect of liming on the flow of recently photosynthesized carbon to rhizosphere soil was studied using 13CO2 pulse labelling, in an upland grassland ecosystem in Scotland. The use of 13C enabled detection, in the field, of the effect of a 4\uffe2\uff80\uff90year liming period of selected soil plots on C allocation from plant biomass to soil, in comparison with unlimed plots. Photosynthetic rates and carbon turnover were higher in plants grown in limed soils than in those from unlimed plots. Higher \uffce\uffb413C\uffe2\uff80\uffb0 values were detected in shoots from limed plants than in those from unlimed plants in samples clipped within 15 days of the end of pulse labelling. Analysis of the aboveground plant production corresponding to the 4\uffe2\uff80\uff90year period of liming indicated that the standing biomass was higher in plots that received lime. Lower \uffce\uffb413C\uffe2\uff80\uffb0 values in limed roots compared with unlimed roots were found, whereas no significant difference was detected between soil samples. Extrapolation of our results indicated that more C has been lost through the soil than has been gained via photosynthetic assimilation because of pasture liming in Scotland during the period 1990\uffe2\uff80\uff931998. However, the uncertainty associated with such extrapolation based on this single study is high and these estimates are provided only to set our findings in the broader context of national soil carbon emissions.</p>", "keywords": ["2. Zero hunger", "/dk/atira/pure/subjectarea/asjc/2300/2306", "name=Global and Planetary Change", "/dk/atira/pure/subjectarea/asjc/2300/2304", "550", "rhizosphere soil", "liming", "04 agricultural and veterinary sciences", "15. Life on land", "630", "upland grassland", "name=Environmental Chemistry", "carbon pools", "name=Ecology", "0401 agriculture", " forestry", " and fisheries", "name=General Environmental Science", "carbon turnover", "/dk/atira/pure/subjectarea/asjc/2300/2303", "13C", "/dk/atira/pure/subjectarea/asjc/2300/2300"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2486.2004.00883.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2004.00883.x", "name": "item", "description": "10.1111/j.1365-2486.2004.00883.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2004.00883.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-12-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=%2Fdk%2Fatira%2Fpure%2Fsubjectarea%2Fasjc%2F2300%2F2304&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=%2Fdk%2Fatira%2Fpure%2Fsubjectarea%2Fasjc%2F2300%2F2304&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=%2Fdk%2Fatira%2Fpure%2Fsubjectarea%2Fasjc%2F2300%2F2304&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=%2Fdk%2Fatira%2Fpure%2Fsubjectarea%2Fasjc%2F2300%2F2304&offset=2", "hreflang": "en-US"}], "numberMatched": 2, "numberReturned": 2, "distributedFeatures": [], "timeStamp": "2026-06-24T10:29:05.002103Z"}