A dramatic evolution of fruit size has accompanied the domestication and improvement of fruit-bearing crop species. In tomato (Solanum lycopersicum), naturally occurring cis-regulatory mutations in the genes of the CLAVATA-WUSCHEL signaling pathway have led to a significant increase in fruit size generating enlarged meristems that lead to flowers with extra organs and bigger fruits. In this work, by combining mapping-by-sequencing and CRISPR/Cas9 genome editing methods, we isolatedEXCESSIVE NUMBER OF FLORAL ORGANS(ENO), an AP2/ERF transcription factor which regulates floral meristem activity. Thus, theENOgene mutation gives rise to plants that yield larger multilocular fruits due to an increased size of the floral meristem. Genetic analyses indicate thatenoexhibits synergistic effects with mutations at theLOCULE NUMBER(encodingSlWUS) andFASCIATED(encodingSlCLV3) loci, two central players in the evolution of fruit size in the domestication of cultivated tomatoes. Our findings reveal that anenomutation causes a substantial expansion ofSlWUSexpression domains in a flower-specific manner. In vitro binding results show that ENO is able to interact with the GGC-box cis-regulatory element within theSlWUSpromoter region, suggesting that ENO directly regulatesSlWUSexpression domains to maintain floral stem-cell homeostasis. Furthermore, the study of natural allelic variation of theENOlocus proved that a cis-regulatory mutation in the promoter ofENOhad been targeted by positive selection during the domestication process, setting up the background for significant increases in fruit locule number and fruit size in modern tomatoes.
", "keywords": ["0301 basic medicine", "570", "Floral meristem", "[SPI] Engineering Sciences [physics]", "[SDV]Life Sciences [q-bio]", "Meristem", "Quantitative Trait Loci", "Genes", " Plant", "CLAVATA/WUSCHEL regulatory network", "Domestication", "[SPI]Engineering Sciences [physics]", "03 medical and health sciences", "Solanum lycopersicum", "Gene Expression Regulation", " Plant", "AP2/ERF transcription factor", "Promoter Regions", " Genetic", "Cell Proliferation", "Plant Proteins", "580", "Homeodomain Proteins", "2. Zero hunger", "Tomato (Solanum lycopersicum)", "0303 health sciences", "Stem Cells", "Biological Sciences", "15. Life on land", "fruit size", "Crop Production", "[SDV] Life Sciences [q-bio]", "CLAVATA-WUSCHEL regulatory network", "GENETICA", "Fruit", "Mutation", "Fruit size", "floral meristem", "Transcription Factors"]}, "links": [{"href": "https://pnas.org/doi/pdf/10.1073/pnas.1913688117"}, {"href": "https://doi.org/10.1073/pnas.1913688117"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Proceedings%20of%20the%20National%20Academy%20of%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1073/pnas.1913688117", "name": "item", "description": "10.1073/pnas.1913688117", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1073/pnas.1913688117"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-16T00:00:00Z"}}, {"id": "10.1016/j.watres.2018.06.016", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:17:57Z", "type": "Journal Article", "created": "2018-06-14", "title": "High-solids anaerobic digestion model for homogenized reactors", "description": "During high-solids anaerobic digestion (HS-AD) of the organic fraction of municipal solid waste (OFMSW), an important total solid (TS) removal occurs, leading to the modification of the reactor content mass/volume, in contrast to 'wet' anaerobic digestion (AD). Therefore, HS-AD mathematical simulations need to be approached differently than 'wet' AD simulations. This study aimed to develop a modelling tool based on the anaerobic digestion model 1 (ADM1) capable of simulating the TS and the reactor mass/volume dynamics in HS-AD of OFMSW. Four hypotheses were used, including the effects of apparent concentrations at high TS. The model simulated adequately HS-AD of OFMSW in batch and continuous mode, particularly the evolution of TS, reactor mass, ammonia and volatile fatty acids. By adequately simulating the reactor content mass/volume and the TS, the HS-AD model might bring further insight about potentially inhibitory mechanisms (i.e. NH3 buildup and/or acidification) occurring in HS-AD of OFMSW.", "keywords": ["[SDE] Environmental Sciences", "ADM1", "570", "[SDV]Life Sciences [q-bio]", "High-solids anaerobic digestion", " ADM1", " Reactor mass simulation", " Total solids", " Apparent concentrations", "0211 other engineering and technologies", "High-solids anaerobic digestion", "02 engineering and technology", "Solid Waste", "01 natural sciences", "7. Clean energy", "Reactor mass simulation", "total solids", "12. Responsible consumption", "high-solids anaerobic digestion", "Bioreactors", "Ammonia", "Apparent concentrations", "reactor mass simulation", "Anaerobiosis", "0105 earth and related environmental sciences", "apparent concentrations", "Total solids", "Models", " Theoretical", "Fatty Acids", " Volatile", "6. Clean water", "Refuse Disposal", "[SDV] Life Sciences [q-bio]", "[SDE]Environmental Sciences"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/718585/1/1-s2.0-S0043135418304603-main.pdf"}, {"href": "https://doi.org/10.1016/j.watres.2018.06.016"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2018.06.016", "name": "item", "description": "10.1016/j.watres.2018.06.016", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2018.06.016"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-10-01T00:00:00Z"}}, {"id": "10.1016/j.watres.2019.114932", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:17:57Z", "type": "Journal Article", "created": "2019-07-30", "title": "Assessing practical identifiability during calibration and cross-validation of a structured model for high-solids anaerobic digestion", "description": "High-solids anaerobic digestion (HS-AD) of the organic fraction of municipal solid waste (OFMSW) is operated at a total solid (TS) content\u202f\u2265\u202f10% to enhance the waste treatment economy, though it might be associated to free ammonia (NH3) inhibition. This study aimed to calibrate and cross-validate a HS-AD model for homogenized reactors in order to assess the effects of high NH3 levels in HS-AD of OFMSW, but also to evaluate the suitability of the reversible non-competitive inhibition function to reproduce the effect of NH3 on the main acetogenic and methanogenic populations. The practical identifiability of structural/biochemical parameters (i.e. 35) and initial conditions (i.e. 32) was evaluated using batch experiments at different TS and/or inoculum-to-substrate ratios. Variance-based global sensitivity analysis and approximate Bayesian computation were used for parameter optimization. The experimental data in this study permitted to estimate up to 8 biochemical parameters, whereas the rest of parameters and biomass contents were poorly identifiable. The study also showed the relatively high levels of NH3 (i.e. up to 2.3\u202fg\u202fN/L) and ionic strength (i.e. up to 0.9\u202fM) when increasing TS in HS-AD of OFMSW. However, the NH3 non-competitive function was unable to capture the acetogenic/methanogenic inhibition. Therefore, the calibration emphasized the need for target-oriented experimental data to enhance the practical identifiability and the predictive capabilities of structured HS-AD models, but also the need for further testing the NH3 inhibition function used in these simulations.", "keywords": ["[SDE] Environmental Sciences", "[SDV]Life Sciences [q-bio]", "0207 environmental engineering", "high-solids anaerobic digestion model", "Bayes Theorem", "02 engineering and technology", "Solid Waste", "01 natural sciences", "7. Clean energy", "6. Clean water", "Refuse Disposal", "12. Responsible consumption", "[SDV] Life Sciences [q-bio]", "High-solids anaerobic digestion model", " ammonia inhibition", " ionic strength", " global sensitivity analysis", " approximate bayesian computation", "Bioreactors", "global sensitivity analysis", "[SDE]Environmental Sciences", "Calibration", "High-solids anaerobic digestion model", "Anaerobiosis", "ionic strength", "Methane", "ammonia inhibition", "approximate bayesian computation", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/757589/1/Post-print%20for%20IRIS.pdf"}, {"href": "https://hal.inrae.fr/hal-02623443/file/S0043135419307067.pdf"}, {"href": "https://doi.org/10.1016/j.watres.2019.114932"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2019.114932", "name": "item", "description": "10.1016/j.watres.2019.114932", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2019.114932"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-11-01T00:00:00Z"}}, {"id": "10.1017/wsc.2017.33", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:02Z", "type": "Journal Article", "created": "2017-09-01", "title": "Weed Abundance And Community Composition Following A Long-Term Organic Vegetable Cropping Systems Experiment", "description": "Weed management is a major constraint in organic cropping systems. In 2004, the Cornell Organic Vegetable Cropping Systems Experiment was established in central New York state using a split-plot randomized complete block design with two crop rotation entry points (split-plot factor). Four organic vegetable cropping systems that varied in cropping intensity and tillage (main plot factor) were compared: (1) intensive, (2) intermediate, (3) bio-extensive, and (4) ridge tillage. The basic crop rotation was cabbage, lettuce, potato, and winter squash, with additional short-season crops in the intensive system and with cover crops and fallow substituted for cabbage and potato in the bio-extensive system. In 2014, two uniformity trials were conducted in which oat and then a mixture of sorghum-sudangrass plus Japanese millet were grown uniformly over the entire experiment. Prior to sowing oat, soil samples were collected from each plot and an emergence bioassay was conducted to assess the soil weed seedbank. Crop biomass, weed density, and weed biomass were sampled in the uniformity crops. Soil weed seedbank density was three to four times greater in the intensive, intermediate, and ridge-tillage systems than in the bio-extensive system. The bio-extensive system also had lower weed density and weed biomass in the oat uniformity trial compared with the other three systems. Oat biomass did not differ between the cropping systems. Weed density and biomass in oat were also affected by the crop rotation entry point. Cropping system legacy effects on weed abundance and community composition were greater in the oat than in the sorghum-sudangrass plus Japanese millet uniformity trial. Our results illustrate the effects of different organic vegetable production practices on weed community structure and highlight the value of tilled fallow periods, cover crops, and prevention of weed seed rain for reducing weed populations.
", "keywords": ["seedbank", "[SDE] Environmental Sciences", "2. Zero hunger", "[SDV]Life Sciences [q-bio]", "04 agricultural and veterinary sciences", "15. Life on land", "630", "emergence bioassay", "uniformity trial", "[SDV] Life Sciences [q-bio]", "bio-extensive", "[SDE]Environmental Sciences", "tillage", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "0401 agriculture", " forestry", " and fisheries", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "cover crops", "legacy effects"]}, "links": [{"href": "https://doi.org/10.1017/wsc.2017.33"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Weed%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1017/wsc.2017.33", "name": "item", "description": "10.1017/wsc.2017.33", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1017/wsc.2017.33"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-09-01T00:00:00Z"}}, {"id": "2078.1/293942", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:26:29Z", "type": "Journal Article", "created": "2024-09-09", "title": "Rainfall Impacts Dissolved Organic Matter and Cation Export From Permafrost Catchments and a Glacial River During Late Summer in Northeast Greenland", "description": "ABSTRACTOngoing and amplified climate change in the Arctic is leading to glacier retreat and to the exposure of an ever\uffe2\uff80\uff90larger portion of non\uffe2\uff80\uff90glaciated permafrost\uffe2\uff80\uff90dominated landscapes. Warming will also cause more precipitation to fall as rain, further enhancing the thaw of previously frozen ground. Yet, the impact of those perturbations on the geochemistry of Arctic rivers remains a subject of debate. Here, we determined the geochemical composition of waters from various contrasting non\uffe2\uff80\uff90glacial permafrost catchments and investigated their impact on a glacially dominated river, the Zackenberg River (Northeast Greenland), during late summer (August 2019). We also studied the effect of rainfall on the geochemistry of the Zackenberg River, its non\uffe2\uff80\uff90glacial tributaries, and a nearby independent non\uffe2\uff80\uff90glacial headwater stream Gr\uffc3\uffa6nse. We analyzed water properties, quantified and characterized dissolved organic matter (DOM) using absorbance and fluorescence spectroscopy and radiocarbon isotopes, and set this alongside analyses of the major cations (Ca, Mg, Na, and K), dissolved silicon (Si), and germanium/silicon ratios (Ge/Si). The glacier\uffe2\uff80\uff90fed Zackenberg River contained low concentrations of major cations, dissolved Si and dissolved organic carbon (DOC), and a Ge/Si ratio typical of bulk rock. Glacial DOM was enriched in protein\uffe2\uff80\uff90like fluorescent DOM and displayed relatively depleted radiocarbon values (i.e., old DOM). Non\uffe2\uff80\uff90glacial streams (i.e., tributaries and Gr\uffc3\uffa6nse) had higher concentrations of major cations and DOC and DOM enriched in aromatic compounds. They showed a wide range of values for radiocarbon, Si and Ge/Si ratios associated with variable contributions of surface runoff relative to deep active layer leaching. Before the rain event, Zackenberg tributaries did not contribute notably to the solute export of the Zackenberg River, and supra\uffe2\uff80\uff90permafrost ground waters governed the supply of solutes in Zackenberg tributaries and Gr\uffc3\uffa6nse stream. After the rain event, surface runoff modified the composition of Gr\uffc3\uffa6nse stream, and non\uffe2\uff80\uff90glacial tributaries strongly increased their contribution to the Zackenberg River solute export. Our results show that summer rainfall events provide an additional source of DOM and Si\uffe2\uff80\uff90rich waters from permafrost\uffe2\uff80\uff90underlain catchments to the discharge of glacially dominated rivers. This suggests that the magnitude and composition of solute exports from Arctic rivers are modulated by permafrost thaw and summer rain events. This event\uffe2\uff80\uff90driven solute supply will likely impact the carbon cycle in rivers, estuaries, and oceans and should be included into future predictions of carbon balance in these vulnerable Arctic systems.We studied the combined effects of reduced tillage and animal manure on soil structure and hydraulic conductivity (K) in the 2\uffe2\uff80\uff9310 and 12\uffe2\uff80\uff9320\uffc2\uffa0cm layers in a loamy soil. The study was performed at the end of a 7\uffe2\uff80\uff90yr field trial and included three tillage treatments (mouldboard ploughing until 25\uffc2\uffa0cm depth: MP, shallow tillage until 12\uffc2\uffa0cm depth: ST, no\uffe2\uff80\uff90till: NT) and two fertilizer application treatments (mineral or poultry manure). Soil structure was assessed through bulk density (\uffcf\uff81b), micromorphological and macropore\uffe2\uff80\uff90space characteristics. K was measured in situ at \uffe2\uff88\uff920.6, \uffe2\uff88\uff920.2 and \uffe2\uff88\uff920.05\uffc2\uffa0kPa. Untilled layers had a vermicular microstructure resulting from earthworm activity, whereas tilled layers displayed a mixture of crumb and channel microstructures. Untilled layers had the highest \uffcf\uff81b and twice as much lower total macroporosity area (pores\uffc2\uffa0>\uffc2\uffa0240\uffc2\uffa0\uffce\uffbcm in equivalent diameter) than tilled layers, reflected by the smallest area of macropores 310\uffe2\uff80\uff932000\uffc2\uffa0\uffce\uffbcm in diameter and the smallest area of large complex macropores. K under untilled layers was 12\uffe2\uff80\uff9362% lower than that under tilled layers, but differences were statistically significant only at \uffe2\uff88\uff920.05\uffc2\uffa0kPa in the 2\uffe2\uff80\uff9310\uffc2\uffa0cm. No significant interaction between tillage and nutrient application treatments was detected for all properties. Compared with mineral fertilizer, poultry manure resulted in a similar \uffcf\uff81b but 20% greater total macroporosity area and 30% higher K at \uffe2\uff88\uff920.2\uffc2\uffa0kPa. Overall, the sensitivity of soil structure and K to poultry manure were relatively small compared with tillage. We suggest that cultivation practices other than animal manure application are needed to improve physical properties under reduced tillage.
", "keywords": ["[SDE] Environmental Sciences", "2. Zero hunger", "macroporosity", "[SDV]Life Sciences [q-bio]", "organic fertilization", "No-tillage", "600", "04 agricultural and veterinary sciences", "micromorphology", "15. Life on land", "630", "6. Clean water", "[SDV] Life Sciences [q-bio]", "image analysis", "[SDE]Environmental Sciences", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.1111/sum.12049"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Use%20and%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/sum.12049", "name": "item", "description": "10.1111/sum.12049", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/sum.12049"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-05-06T00:00:00Z"}}, {"id": "10.1093/jxb/erab082", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:02Z", "type": "Journal Article", "created": "2021-03-05", "title": "A common bean truncated CRINKLY4 kinase controls gene-for-gene resistance to the fungus Colletotrichum lindemuthianum", "description": "AbstractIdentifying the molecular basis of resistance to pathogens is critical to promote a chemical-free cropping system. In plants, nucleotide-binding leucine-rich repeat constitute the largest family of disease resistance (R) genes, but this resistance can be rapidly overcome by the pathogen, prompting research into alternative sources of resistance. Anthracnose, caused by the fungus Colletotrichum lindemuthianum, is one of the most important diseases of common bean. This study aimed to identify the molecular basis of Co-x, an anthracnose R gene conferring total resistance to the extremely virulent C. lindemuthianum strain 100. To that end, we sequenced the Co-x 58 kb target region in the resistant JaloEEP558 (Co-x) common bean and identified KTR2/3, an additional gene encoding a truncated and chimeric CRINKLY4 kinase, located within a CRINKLY4 kinase cluster. The presence of KTR2/3 is strictly correlated with resistance to strain 100 in a diversity panel of common beans. Furthermore, KTR2/3 expression is up-regulated 24 hours post-inoculation and its transient expression in a susceptible genotype increases resistance to strain 100. Our results provide evidence that Co-x encodes a truncated and chimeric CRINKLY4 kinase probably resulting from an unequal recombination event that occurred recently in the Andean domesticated gene pool. This atypical R gene may act as a decoy involved in indirect recognition of a fungal effector.
", "keywords": ["Phaseolus", "2. Zero hunger", "0301 basic medicine", "anthracnose", "0303 health sciences", "[SDV]Life Sciences [q-bio]", "610", "Chromosome Mapping", "Genes", " Plant", "Phaseolus vulgaris", "630", "NLR", "[SDV] Life Sciences [q-bio]", "03 medical and health sciences", "disease resistance gene", "Colletotrichum", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "CRINKLY4 kinase", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "Common bean", "Common bean", " Phaseolus vulgaris", " NLR", " disease resistance gene", " CRINKLY4 kinase", " anthracnose", " unequal crossing-over", "unequal crossing-over", "Plant Diseases"]}, "links": [{"href": "http://academic.oup.com/jxb/article-pdf/72/10/3569/37799399/erab082.pdf"}, {"href": "https://doi.org/10.1093/jxb/erab082"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Experimental%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/jxb/erab082", "name": "item", "description": "10.1093/jxb/erab082", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/jxb/erab082"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-03-06T00:00:00Z"}}, {"id": "10.1038/s41477-023-01583-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:23Z", "type": "Journal Article", "created": "2023-12-04", "title": "Subtilase-mediated biogenesis of the expanded family of SERINE RICH ENDOGENOUS PEPTIDES", "description": "Plant signalling peptides are typically released from larger precursors by proteolytic cleavage to regulate plant growth, development and stress responses. Recent studies reported the characterization of a divergent family of Brassicaceae-specific peptides, SERINE RICH ENDOGENOUS PEPTIDES (SCOOPs), and their perception by the leucine-rich repeat receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2). Here, we reveal that the SCOOP family is highly expanded, containing at least 50 members in the Columbia-0 reference Arabidopsis thaliana genome. Notably, perception of these peptides is strictly MIK2-dependent. How bioactive SCOOP peptides are produced, and to what extent their perception is responsible for the multiple physiological roles associated with MIK2 are currently unclear. Using N-terminomics, we validate the N-terminal cleavage site of representative PROSCOOPs. The cleavage sites are determined by conserved motifs upstream of the minimal SCOOP bioactive epitope. We identified subtilases necessary and sufficient to process PROSCOOP peptides at conserved cleavage motifs. Mutation of these subtilases, or their recognition motifs, suppressed PROSCOOP cleavage and associated overexpression phenotypes. Furthermore, we show that higher-order mutants of these subtilases show phenotypes reminiscent of mik2 null mutant plants, consistent with impaired PROSCOOP biogenesis, and demonstrating biological relevance of SCOOP perception by MIK2. Together, this work provides insights into the molecular mechanisms underlying the functions of the recently identified SCOOP peptides and their receptor MIK2.", "keywords": ["570", "Arabidopsis Proteins", "[SDV]Life Sciences [q-bio]", "Arabidopsis", "Receptors", " Cell Surface", "580 Plants (Botany)", "[SDV] Life Sciences [q-bio]", "10126 Department of Plant and Microbial Biology", "1110 Plant Science", "Taverne", "Brassicaceae", "Serine", "Life Science", "10211 Zurich-Basel Plant Science Center", "Peptides", "Protein Kinases"]}, "links": [{"href": "https://www.nature.com/articles/s41477-023-01583-x.pdf"}, {"href": "https://hal.science/hal-04394015/file/NaturePlants_subtilase_scoop_2023_revised.pdf"}, {"href": "https://doi.org/10.1038/s41477-023-01583-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Plants", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41477-023-01583-x", "name": "item", "description": "10.1038/s41477-023-01583-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41477-023-01583-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-04T00:00:00Z"}}, {"id": "10.1038/s41467-019-14197-9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:22Z", "type": "Journal Article", "created": "2020-01-24", "title": "High-quality genome sequence of white lupin provides insight into soil exploration and seed quality", "description": "AbstractWhite lupin (Lupinus albus L.) is an annual crop cultivated for its protein-rich seeds. It is adapted to poor soils due to the production of cluster roots, which are made of dozens of determinate lateral roots that drastically improve soil exploration and nutrient acquisition (mostly phosphate). Using long-read sequencing technologies, we provide a high-quality genome sequence of a cultivated accession of white lupin (2n\uffe2\uff80\uff89=\uffe2\uff80\uff8950, 451\uffe2\uff80\uff89Mb), as well as de novo assemblies of a landrace and a wild relative. We describe a modern accession displaying increased soil exploration capacity through early establishment of lateral and cluster roots. We also show how seed quality may have been impacted by domestication in term of protein profiles and alkaloid content. The availability of a high-quality genome assembly together with companion genomic and transcriptomic resources will enable the development of modern breeding strategies to increase and stabilize white lupin yield.
", "keywords": ["Repetitive Sequences", " Nucleic Acid/genetics", "0301 basic medicine", "[SDV]Life Sciences [q-bio]", "Plant Roots/genetics", "Gene Dosage", "Plant Science", "Crop", "Alkaloids/chemistry", "Plant Roots", "Gene", "Repetitive Sequences", "630", "Agricultural and Biological Sciences", "Domestication", "Soil", "Models", "Symbiotic Nitrogen Fixation in Legumes", "Gene Duplication", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "http://aims.fao.org/aos/agrovoc/c_3224", "Plant Proteins/metabolism", "Plant Proteins", "2. Zero hunger", "0303 health sciences", "Genome", "Q", "http://aims.fao.org/aos/agrovoc/c_27583", "Life Sciences", "Transcriptome/genetics", "http://aims.fao.org/aos/agrovoc/c_92382", "Polymorphism", " Single Nucleotide/genetics", "Lupinus", "[SDV] Life Sciences [q-bio]", "Protein Crop", "Seeds", "http://aims.fao.org/aos/agrovoc/c_5956", "White (mutation)", "Single Nucleotide/genetics", "Sequence Analysis", "Genome", " Plant", "expression des g\u00e8nes", "http://aims.fao.org/aos/agrovoc/c_4464", "Synteny/genetics", "Evolution", "Lupin Seeds", "Science", "Centromere", "Lupinus/genetics", "Polymorphism", " Single Nucleotide", "Article", "g\u00e9nomique", "Evolution", " Molecular", "Evolution and Nutritional Properties of Lupin Seeds", "physiologie v\u00e9g\u00e9tale", "03 medical and health sciences", "Alkaloids", "Genetic", "Nucleic Acid/genetics", "Seeds/physiology", "Centromere/genetics", "Genetics", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "Polymorphism", "Biology", "Ecology", " Evolution", " Behavior and Systematics", "Repetitive Sequences", " Nucleic Acid", "Sequence assembly", "http://aims.fao.org/aos/agrovoc/c_25189", "Ecotype", "Models", " Genetic", "g\u00e9nome", "Botany", "Molecular", "Genetic Variation", "Molecular Sequence Annotation", "Plant", "DNA", "Sequence Analysis", " DNA", "s\u00e9quence nucl\u00e9otidique", "15. Life on land", "http://aims.fao.org/aos/agrovoc/c_27527", "Agronomy", "Plant Leaves", "Evolution and Ecology of Endophyte-Grass Symbiosis", "Lupinus albus", "FOS: Biological sciences", "Genomic Structural Variation", "Plant Leaves/metabolism", "Gene expression", "Transcriptome", "am\u00e9lioration des plantes"]}, "links": [{"href": "https://www.nature.com/articles/s41467-019-14197-9.pdf"}, {"href": "https://doi.org/10.1038/s41467-019-14197-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-019-14197-9", "name": "item", "description": "10.1038/s41467-019-14197-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-019-14197-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-24T00:00:00Z"}}, {"id": "10.1088/1748-9326/aa9c5c", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:58Z", "type": "Journal Article", "created": "2017-11-22", "title": "Gap assessment in current soil monitoring networks across Europe for measuring soil functions", "description": "Soil is the most important natural resource for life on Earth after water. Given its fundamental role in sustaining the human population, both the availability and quality of soil must be managed sustainably and protected. To ensure sustainable management we need to understand the intrinsic functional capacity of different soils across Europe and how it changes over time. Soil monitoring is needed to support evidence-based policies to incentivise sustainable soil management. To this aim, we assessed which soil attributes can be used as potential indicators of five soil functions; (1) primary production, (2) water purification and regulation, (3) carbon sequestration and climate regulation, (4) soil biodiversity and habitat provisioning and (5) recycling of nutrients. We compared this list of attributes to existing national (regional) and EU-wide soil monitoring networks. The overall picture highlighted a clearly unbalanced dataset, in which predominantly chemical soil parameters were included, and soil biological and physical attributes were severely under represented. Methods applied across countries for indicators also varied. At a European scale, the LUCAS-soil survey was evaluated and again confirmed a lack of important soil biological parameters, such as C mineralisation rate, microbial biomass and earthworm community, and soil physical measures such as bulk density. In summary, no current national or European monitoring system exists which has the capacity to quantify the five soil functions and therefore evaluate multi-functional capacity of a soil and in many countries no data exists at all. This paper calls for the addition of soil biological and some physical parameters within the LUCAS-soil survey at European scale and for further development of national soil monitoring schemes.", "keywords": ["[SDE] Environmental Sciences", "570", "[SDV]Life Sciences [q-bio]", "Science", "QC1-999", "soil functions;soil monitoring networks;soil attributes;Europe", "Environmental technology. Sanitary engineering", "630", "12. Responsible consumption", "GE1-350", "TD1-1066", "2. Zero hunger", "Physics", "Q", "04 agricultural and veterinary sciences", "soil functions", "15. Life on land", "S590 Soill / Talajtan", "soil monitoring networks", "6. Clean water", "[SDV] Life Sciences [q-bio]", "Europe", "Environmental sciences", "soil attributes", "13. Climate action", "[SDE]Environmental Sciences", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://hal.inrae.fr/hal-02622332/file/2017_Leeuwen_Environmental%20Research%20Letters_1.pdf"}, {"href": "https://doi.org/10.1088/1748-9326/aa9c5c"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Research%20Letters", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1088/1748-9326/aa9c5c", "name": "item", "description": "10.1088/1748-9326/aa9c5c", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1088/1748-9326/aa9c5c"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-12-01T00:00:00Z"}}, {"id": "10.1038/s41559-018-0694-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:24Z", "type": "Journal Article", "created": "2018-10-19", "title": "Isotopic evidence for oligotrophication of terrestrial ecosystems", "description": "Human societies depend on an Earth system that operates within a constrained range of nutrient availability, yet the recent trajectory of terrestrial nitrogen (N) availability is uncertain. Examining patterns of foliar N concentrations and isotope ratios (\u03b415N) from more than 43,000 samples acquired over 37\u2009years, here we show that foliar N concentration declined by 9% and foliar \u03b415N declined by 0.6-1.6\u2030. Examining patterns across different climate spaces, foliar \u03b415N declined across the entire range of mean annual temperature and mean annual precipitation tested. These results suggest declines in N supply relative to plant demand at the global scale. In all, there are now multiple lines of evidence of declining N availability in many unfertilized terrestrial ecosystems, including declines in \u03b415N of tree rings and leaves from herbarium samples over the past 75-150\u2009years. These patterns are consistent with the proposed consequences of elevated atmospheric carbon dioxide and longer growing seasons. These declines will limit future terrestrial carbon uptake and increase nutritional stress for herbivores.", "keywords": ["0106 biological sciences", "570", "Nitrogen", "[SDV]Life Sciences [q-bio]", "577", "terrestrial nitrogen", "Nutritional stress", "551", "01 natural sciences", "oligotrophication", "Isotopes", "https://purl.org/becyt/ford/1.6", "Terrestrial carbon uptake", "https://purl.org/becyt/ford/1", "Ecosystem", "580", "2. Zero hunger", "Nitrogen Isotopes", "terrestrial ecosystems", "isotopic", "Eutrophication", "Plants", "15. Life on land", "sylviculture", "Nitrogen; Nitrogen Isotopes; Plants; Ecosystem; Eutrophication", "[SDV] Life Sciences [q-bio]", "element cycles", "foliar", "13. Climate action", "nutrient availability"]}, "links": [{"href": "https://cris.unibo.it/bitstream/11585/704621/3/Craine_2018_isotopic.pdf"}, {"href": "https://www.nature.com/articles/s41559-018-0694-0.pdf"}, {"href": "https://doi.org/10.1038/s41559-018-0694-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Ecology%20%26amp%3B%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41559-018-0694-0", "name": "item", "description": "10.1038/s41559-018-0694-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41559-018-0694-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-10-22T00:00:00Z"}}, {"id": "10.1038/s41586-023-05791-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:25Z", "type": "Journal Article", "created": "2023-03-08", "title": "The giant diploid faba genome unlocks variation in a global protein crop", "description": "AbstractIncreasing the proportion of locally produced plant protein in currently meat-rich diets could substantially reduce greenhouse gas emissions and loss of biodiversity1. However, plant protein production is hampered by the lack of a cool-season legume equivalent to soybean in agronomic value2. Faba bean (Vicia fabaL.) has a high yield potential and is well suited for cultivation in temperate regions, but genomic resources are scarce. Here, we report a high-quality chromosome-scale assembly of the faba bean genome and show that it has expanded to a massive 13\uffe2\uff80\uff89Gb in size through an imbalance between the rates of amplification and elimination of retrotransposons and satellite repeats. Genes and recombination events are evenly dispersed across chromosomes and the gene space is remarkably compact considering the genome size, although with substantial copy number variation driven by tandem duplication. Demonstrating practical application of the genome sequence, we develop a targeted genotyping assay and use high-resolution genome-wide association analysis to dissect the genetic basis of seed size and hilum colour. The resources presented constitute a genomics-based breeding platform for faba bean, enabling breeders and geneticists to accelerate the\uffc2\uffa0improvement of sustainable protein production across the\uffc2\uffa0Mediterranean, subtropical and northern temperate agroecological zones.Biomass from dedicated crops is expected to contribute significantly to the replacement of fossil resources. However, sustainable bioenergy cropping systems must provide high biomass production and low environmental impacts. This study aimed at quantifying biomass production, nutrient removal, expected ethanol production, and greenhouse gas (GHG) balance of six bioenergy crops: Miscanthus\uffc2\uffa0\uffc3\uff97\uffc2\uffa0giganteus, switchgrass, fescue, alfalfa, triticale, and fiber sorghum. Biomass production and N, P, K balances (input\uffe2\uff80\uff90output) were measured during 4\uffc2\uffa0years in a long\uffe2\uff80\uff90term experiment, which included two nitrogen fertilization treatments. These results were used to calculate a posteriori \uffe2\uff80\uff98optimized\uffe2\uff80\uff99 fertilization practices, which would ensure a sustainable production with a nil balance of nutrients. A modified version of the cost/benefit approach proposed by Crutzen et\uffc2\uffa0al. (2008), comparing the GHG emissions resulting from N\uffe2\uff80\uff90P\uffe2\uff80\uff90K fertilization of bioenergy crops and the GHG emissions saved by replacing fossil fuel, was applied to these \uffe2\uff80\uff98optimized\uffe2\uff80\uff99 situations. Biomass production varied among crops between 10.0 (fescue) and 26.9\uffc2\uffa0t\uffc2\uffa0DM\uffc2\uffa0ha\uffe2\uff88\uff921\uffc2\uffa0yr\uffe2\uff88\uff921 (miscanthus harvested early) and the expected ethanol production between 1.3 (alfalfa) and 6.1\uffc2\uffa0t\uffc2\uffa0ha\uffe2\uff88\uff921\uffc2\uffa0yr\uffe2\uff88\uff921 (miscanthus harvested early). The cost/benefit ratio ranged from 0.10 (miscanthus harvested late) to 0.71 (fescue); it was closely correlated with the N/C ratio of the harvested biomass, except for alfalfa. The amount of saved CO2 emissions varied from 1.0 (fescue) to 8.6\uffc2\uffa0t CO2eq\uffc2\uffa0ha\uffe2\uff88\uff921\uffc2\uffa0yr\uffe2\uff88\uff921 (miscanthus harvested early or late). Due to its high biomass production, miscanthus was able to combine a high production of ethanol and a large saving of CO2 emissions. Miscanthus and switchgrass harvested late gave the best compromise between low N\uffe2\uff80\uff90P\uffe2\uff80\uff90K requirements, high GHG saving per unit of biomass, and high productivity per hectare.
", "keywords": ["legume crops", "2. Zero hunger", "660", "[SDV]Life Sciences [q-bio]", "0211 other engineering and technologies", "02 engineering and technology", "15. Life on land", "7. Clean energy", "nitrogen", "lignocelluloses", "12. Responsible consumption", "[SDV] Life Sciences [q-bio]", "greenhouse gas", "13. Climate action", "8. Economic growth", "0202 electrical engineering", " electronic engineering", " information engineering", "biofuel", "nutrient use Efficiency", "biomasse", "ethanol", "C3 crops"]}, "links": [{"href": "https://hal.science/hal-01173307/file/Cadoux_etal_2014.pdf"}, {"href": "https://doi.org/10.1111/gcbb.12065"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/GCB%20Bioenergy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcbb.12065", "name": "item", "description": "10.1111/gcbb.12065", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcbb.12065"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-04-12T00:00:00Z"}}, {"id": "10.1111/gcbb.12158", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-24T16:19:25Z", "type": "Journal Article", "created": "2014-02-05", "title": "Effect Of Physical Weathering On The Carbon Sequestration Potential Of Biochars And Hydrochars In Soil", "description": "AbstractPhysical weathering can modify the stability of biochar after field exposure. The aim of our study was to determine the potential carbon sequestration of the two chars at different timescales. We investigated the modification in composition and stability resulting from physical weathering of two different chars produced (i) at low temperature (250\uffc2\uffa0\uffc2\uffb0C) by hydrothermal carbonization (HTC); and (ii) at high temperature (1200\uffc2\uffa0\uffc2\uffb0C) by gasification (GS) using contrasting feedstocks. Physical weathering of HTC and GS placed on a water permeable canvas was performed through successive wetting/drying and freezing/thawing cycles. Carbon loss was assessed by mass balance. Chemical stability of the remaining material was evaluated as resistance to acid dichromate oxidation, and biological stability was assessed during laboratory incubation. Moreover, we assessed modification in potential priming effects due to physical weathering. Physical weathering induced a carbon loss ranging between 10 and 40% of the total C mass depending on the feedstock. This C loss is most probably related to leaching of small particulate and dissolved compounds. GS produced from maize silage showed the highest C loss. The chemical stability of HTC and GS was unaffected by physical weathering. In contrast, physical weathering strongly increased the biological stability of HTC and GS char produced from maize silage. After physical weathering, the half\uffe2\uff80\uff90life (t1/2) of GS was doubled but only slight increase was noted for those of HTC. During the first weeks of incubation, HTC addition to soil stimulated native soil organic matter (SOM) mineralization (positive priming effect), while the GS addition led to protection of the native SOM against biologic degradation (negative priming effect). Physical weathering led to reduction in these priming effects. Model extrapolations based on our data showed that decadal C sequestration potential of GS and HTC is globally equivalent when all losses including those due to priming and physical weathering were taken into account. However, at century scale only GS may have the potential to increase soil C storage.
", "keywords": ["priming effect", "[SDE] Environmental Sciences", "2. Zero hunger", "[SDV]Life Sciences [q-bio]", "aging", "gasification", "HTC", "04 agricultural and veterinary sciences", "15. Life on land", "carbon sequestration", "01 natural sciences", "630", "hydrothermal carbonization", "[SDV] Life Sciences [q-bio]", "13. Climate action", "soil organic matter", "[SDE]Environmental Sciences", "weathering", "0401 agriculture", " forestry", " and fisheries", "chemical oxidation", "biochar", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1111/gcbb.12158"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/GCB%20Bioenergy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcbb.12158", "name": "item", "description": "10.1111/gcbb.12158", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcbb.12158"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-02-05T00:00:00Z"}}, {"id": "10.1088/1748-9326/8/1/015029", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:58Z", "type": "Journal Article", "created": "2013-03-07", "title": "Selection Of Appropriate Calculators For Landscape-Scale Greenhouse Gas Assessment For Agriculture And Forestry", "description": "This letter is intended to help potential users select the most appropriate calculator for a landscape-scale greenhouse gas (GHG) assessment of activities for agriculture and forestry. Eighteen calculators were assessed. These calculators were designed for different aims and to be used in different geographical areas and they use slightly different accounting methodologies. The classification proposed is based on the main aim of the assessment: raising awareness, reporting, project evaluation or product assessment. When the aims have been clearly formulated, the most suitable calculator can be selected from the comparison tables, taking account of the geographical area and the scope of the calculation as well as the time and skills required for the calculation. The main issues for interpreting GHG assessments are discussed, highlighting the difficulty of comparing the results obtained from different calculators, mainly owing to differences in scope, calculation methods and reporting units. A major problem is the poor accounting for land use change; the calculators are usually able to account satisfactorily for other emission sources. One of the main challenges at landscape-scale level is to produce a realistic assessment of the various production systems as the uncertainty levels are very high. The results should always give some indication of the link between GHG emissions and the productivity of the area, although no single indicator is able to encompass all the services produced by agriculture and forestry (e.g. food, goods, landscape value and revenue).", "keywords": ["550", "[SDV]Life Sciences [q-bio]", "Science", "QC1-999", "indicateur environnemental", "calculators", "710", "AFOLU", "Environmental technology. Sanitary engineering", "01 natural sciences", "630", "12. Responsible consumption", "mitigation", "greenhouse gases", "11. Sustainability", "gaz \u00e0 effet de serre", "GE1-350", "paysage", "climate", "TD1-1066", "agriculture", "0105 earth and related environmental sciences", "changement climatique", "Physics", "Q", "landscape;carbon calculators;greenhouse gases;GHG emissions;AFOLU;mitigation", "04 agricultural and veterinary sciences", "landscape", "15. Life on land", "carbon calculators", "[SDV] Life Sciences [q-bio]", "GHG emissions", "Environmental sciences", "13. Climate action", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://hal.science/hal-01190664/file/Colomb-EnvResLett-2013_%7B85094A8F-159E-4C0A-9FB9-2DA75BDB27B8%7D.pdf"}, {"href": "https://doi.org/10.1088/1748-9326/8/1/015029"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Research%20Letters", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1088/1748-9326/8/1/015029", "name": "item", "description": "10.1088/1748-9326/8/1/015029", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1088/1748-9326/8/1/015029"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-03-01T00:00:00Z"}}, {"id": "10.1111/gcb.13151", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:21Z", "type": "Journal Article", "created": "2015-11-07", "title": "Canopy leaf area of a mature evergreen Eucalyptus woodland does not respond to elevated atmospheric [CO 2 ] but tracks water availability", "description": "AbstractCanopy leaf area, quantified by the leaf area index (L), is a crucial driver of forest productivity, water use and energy balance. Because L responds to environmental drivers, it can represent an important feedback to climate change, but its responses to rising atmospheric [CO2] and water availability of forests have been poorly quantified. We studied canopy leaf area dynamics for 28\uffc2\uffa0months in a native evergreen Eucalyptus woodland exposed to free\uffe2\uff80\uff90air CO2 enrichment (the EucFACE experiment), in a subtropical climate where water limitation is common. We hypothesized that, because of expected stimulation of productivity and water\uffe2\uff80\uff90use efficiency, L should increase with elevated [CO2]. We estimated L from diffuse canopy transmittance, and measured monthly leaf litter production. Contrary to expectation, L did not respond to elevated [CO2]. We found that L varied between 1.10 and 2.20 across the study period. The dynamics of L showed a quick increase after heavy rainfall and a steady decrease during periods of low rainfall. Leaf litter production was correlated to changes in L, both during periods of decreasing L (when no leaf growth occurred) and during periods of increasing L (active shedding of old foliage when new leaf growth occurred). Leaf lifespan, estimated from mean L and total annual litter production, was up to 2\uffc2\uffa0months longer under elevated [CO2] (1.18 vs. 1.01\uffc2\uffa0years; P\uffc2\uffa0=\uffc2\uffa00.05). Our main finding that L was not responsive to elevated CO2 is consistent with other forest FACE studies, but contrasts with the positive response of L commonly predicted by many ecosystem models.
", "keywords": ["[SDE] Environmental Sciences", "0106 biological sciences", "Eucalyptus", "leaf area index", "Atmosphere", "Water", "drought", "Carbon Dioxide", "Forests", "15. Life on land", "phenology", "01 natural sciences", "free-air CO2 enrichment", "6. Clean water", "[SDV] Life Sciences [q-bio]", "Plant Leaves", "13. Climate action", "atmospheric carbon dioxide", "XXXXXX - Unknown", "leaves", "New South Wales", "litter production"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.13151"}, {"href": "https://doi.org/10.1111/gcb.13151"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.13151", "name": "item", "description": "10.1111/gcb.13151", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.13151"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-02-09T00:00:00Z"}}, {"id": "10.1093/jxb/erq249", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:02Z", "type": "Journal Article", "created": "2010-08-27", "title": "Plant Physiology And Proteomics Reveals The Leaf Response To Drought In Alfalfa (Medicago Sativa L.)", "description": "Despite its relevance, protein regulation, metabolic adjustment, and the physiological status of plants under drought is not well understood in relation to the role of nitrogen fixation in nodules. In this study, nodulated alfalfa plants were exposed to drought conditions. The study determined the physiological, metabolic, and proteomic processes involved in photosynthetic inhibition in relation to the decrease in nitrogenase (N(ase)) activity. The deleterious effect of drought on alfalfa performance was targeted towards photosynthesis and N(ase) activity. At the leaf level, photosynthetic inhibition was mainly caused by the inhibition of Rubisco. The proteomic profile and physiological measurements revealed that the reduced carboxylation capacity of droughted plants was related to limitations in Rubisco protein content, activation state, and RuBP regeneration. Drought also decreased amino acid content such as asparagine, and glutamic acid, and Rubisco protein content indicating that N availability limitations were caused by N(ase) activity inhibition. In this context, drought induced the decrease in Rubisco binding protein content at the leaf level and proteases were up-regulated so as to degrade Rubisco protein. This degradation enabled the reallocation of the Rubisco-derived N to the synthesis of amino acids with osmoregulant capacity. Rubisco degradation under drought conditions was induced so as to remobilize Rubisco-derived N to compensate for the decrease in N associated with N(ase) inhibition. Metabolic analyses showed that droughted plants increased amino acid (proline, a major compound involved in osmotic regulation) and soluble sugar (D-pinitol) levels to contribute towards the decrease in osmotic potential (\u03a8(s)). At the nodule level, drought had an inhibitory effect on N(ase) activity. This decrease in N(ase) activity was not induced by substrate shortage, as reflected by an increase in total soluble sugars (TSS) in the nodules. Proline accumulation in the nodule could also be associated with an osmoregulatory response to drought and might function as a protective agent against ROS. In droughted nodules, the decrease in N(2) fixation was caused by an increase in oxygen resistance that was induced in the nodule. This was a mechanism to avoid oxidative damage associated with reduced respiration activity and the consequent increase in oxygen content. This study highlighted that even though drought had a direct effect on leaves, the deleterious effects of drought on nodules also conditioned leaf responsiveness.", "keywords": ["Proteomics", "0301 basic medicine", "570", "Rubisco", "Proteome", "[SDV]Life Sciences [q-bio]", "proteome", "N-2 FIXATION RESPONSE", "drought", "N2 fixation", "03 medical and health sciences", "XANTHOPHYLL CYCLE", "N-2 fixation", "2-CYSTEINE PEROXIREDOXIN", "Nitrogenase", "oxidative stress", "AMINO-ACIDS", "Photosynthesis", "climate", "agriculture", "Plant Proteins", "580", "N remobilization", "2. Zero hunger", "0303 health sciences", "photosynthesis", "PINITOL ACCUMULATION", "Drought", "RIBULOSE-1", "5-BISPHOSPHATE CARBOXYLASE-OXYGENASE", "Water", "BRASSICA-NAPUS", "N(O)-TERT-BUTYLDIMETHYLSILYL DERIVATIVES", "15. Life on land", "Research Papers", "6. Clean water", "Droughts", "[SDV] Life Sciences [q-bio]", "Plant Leaves", "nitrogen fixation", "Oxidative stress", "rubisco", "NITROGEN-FIXATION", "WATER-WATER CYCLE", "Medicago sativa"]}, "links": [{"href": "https://doi.org/10.1093/jxb/erq249"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Experimental%20Botany", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/jxb/erq249", "name": "item", "description": "10.1093/jxb/erq249", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/jxb/erq249"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-08-25T00:00:00Z"}}, {"id": "10.1093/pcp/pcae113", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:03Z", "type": "Journal Article", "created": "2024-11-06", "title": "Population Genomics Reveals Demographic History and Climate Adaptation in Japanese Arabidopsis halleri", "description": "AbstractClimate oscillations in the Quaternary forced species to major latitudinal or altitudinal range shifts. It has been suggested that adaptation concomitant with range shifts plays key roles in species responses during climate oscillations, but the role of selection for local adaptation to climatic changes remains largely unexplored. Here, we investigated population structure, demographic history and signatures of climate-driven selection based on genome-wide polymorphism data of 141 Japanese Arabidopsis halleri individuals, with European ones as outgroups. Coalescent-based analyses suggested a genetic differentiation between Japanese subpopulations since the Last Glacial Period (LGP), which would have contributed to shaping the current pattern of population structure. Population demographic analysis revealed the population size fluctuations in the LGP, which were particularly prominent since the subpopulations started to diverge (\uffe2\uff88\uffbc50, 000 years ago). The ecological niche modeling predicted the geographic or distribution range shifts from southern coastal regions to northern coastal and mountainous areas, possibly in association with the population size fluctuations. Through genome-wide association analyses of bioclimatic variables and selection scans, we investigated whether climate-associated loci are enriched in the extreme tails of selection scans, and demonstrated the prevailing signatures of selection, particularly toward a warmer climate in southern subpopulations and a drier environment in northern subpopulations, which may have taken place during or after the LGP. Our study highlights the importance of integrating climate associations, selection scans and population demographic analyses for identifying genomic signatures of population-specific adaptation, which would also help us predict the evolutionary responses to future climate changes.MicroRNAs (miRNAs) are a class of small non-coding RNAs that play important regulatory roles in plant genomes. While some miRNA genes are deeply conserved, the majority appear to be species-specific, raising the question of how they emerge and integrate into cellular regulatory networks. To address this question, we first performed a detailed annotation of miRNA genes in the closely related Arabidopsis halleri and A. lyrata, then evaluated their phylogenetic conservation across 87 plant species. We then characterized the process by which newly emerged miRNA genes progressively acquire the properties of \uffe2\uff80\uff9ccanonical\uffe2\uff80\uff9d miRNA genes, in terms of size and stability of the hairpin precursor, loading of their cleavage products into Argonaute proteins, and potential to regulate downstream target genes. Analysis of nucleotide polymorphism distribution along the hairpin sequence (stem, mature miRNA, terminal loop) revealed that the selective constraints on recently emerged miRNA genes were initially weak, gradually increasing toward evolutionarily conserved miRNA genes. Our results illustrate the rapid birth-and-death of miRNA genes in plant genomes, and provide a detailed picture of the evolutionary progression toward canonical miRNAs by which a small fraction of de novo formed miRNA genes eventually integrate into \uffe2\uff80\uff9ccore\uffe2\uff80\uff9d biological processes.
Intensive agriculture is dominated by monocultures of high\uffe2\uff80\uff90yielding plants that receive large applications of nitrogen (N) fertilizers to boost plant productivity. However, these systems have low N use efficiency (NUE) as fertilized plants generally take up less than half of the N applied. A large fraction of the remainder N is susceptible to be lost from the agroecosystem generating a cascade of environmental and socio\uffe2\uff80\uff90economic problems. Climate change and projected global increases in fertilizer use pose further risks to N losses and yield stability.
We review and translate concepts from ecology in natural systems to demonstrate that NUE in intensive agroecosystems can be strongly increased by fine\uffe2\uff80\uff90tuning the traits of the plant communities to the levels of N fertilization intensity.
We present key plant traits of importance for N\uffe2\uff80\uff90cycling (architectural, morphological and physiological traits, as well as symbiotic associations and exudation patterns); discuss ecological (with soil fauna and N\uffe2\uff80\uff90cycling microbial communities) and agronomic interactions of this approach; propose interdisciplinary methodologies for future research ranging from pot to global scales; and highlight possible solutions leading to an optimal balance between N fertilizer use and productivity.
Synthesis and applications. By showing the strong links between plant traits and nitrogen (N) cycling, our work opens possibilities to test ecologically informed hypotheses across gradients of soil fertility and N fertilizer management intensity, setting a research agenda for the coming years. Accordingly, the choice of plant species based on their functional traits will play a central role for the development of modern and productive agroecosystems that retain and use N more efficiently.
Soil invertebrate communities represent a significant fraction of global biodiversity and play crucial roles in ecosystems. A number of human activities threaten soil communities, in particular intensive agricultural practices such as pesticide use. However, there is currently no quantitative synthesis of the impacts of pesticides on soil fauna communities.
Here, using a meta\uffe2\uff80\uff90analysis of 54 studies and 294 observations, we quantify pesticide effects on the abundance, biomass, richness and diversity of natural soil fauna communities across a wide range of environmental contexts. We also identify scenarios with the most detrimental effects on soil fauna communities by analysing the effects of different pesticides (herbicides, fungicides, insecticides, broad\uffe2\uff80\uff90spectrum substances and multiple substances), different application rates and temporal extents (short\uffe2\uff80\uff90 or long\uffe2\uff80\uff90term), as well as the response of different functional groups of soil animals (body size categories, presence of exoskeleton).
Pesticides overall decreased the abundance and diversity of soil fauna communities across studies (Grand mean effect size (Hedge's g)\uffe2\uff80\uff89=\uffe2\uff80\uff89\uffe2\uff88\uff920.30\uffe2\uff80\uff89+/\uffe2\uff88\uff92\uffe2\uff80\uff890.16) and had stronger effects on soil fauna diversity than abundance. The most detrimental scenarios involved multiple substances, broad\uffe2\uff80\uff90spectrum substances and insecticides, which significantly decreased soil fauna diversity even at recommended rates. We found no evidence that pesticide effects dampen over time, as short\uffe2\uff80\uff90term and long\uffe2\uff80\uff90term studies exhibited similar mean effect sizes.
Policy implications: Our study highlights that pesticide use has significant detrimental non\uffe2\uff80\uff90target effects on soil biodiversity, eroding a substantial part of global biodiversity and threatening ecosystem health. This provides crucial evidence supporting recent policies, such as the European Green Deal, that aim to reduce pesticide use in agriculture to conserve biodiversity. The detrimental effects of multiple substances revealed here are particularly concerning because realistic pesticide use often combines several substances targeting different pests and diseases over the crop season. We suggest that future guidelines for pesticide registration, restrictions and banning should rely on data able to fully capture the long\uffe2\uff80\uff90term consequences of multiple substances for multiple non\uffe2\uff80\uff90target species in realistic conditions.
The food industry faces numerous challenges to assure provision of tasty and convenient food that possesses extended shelf life and shows long\uffe2\uff80\uff90term high\uffe2\uff80\uff90quality preservation. Research and development of antimicrobial materials for food applications have provided active antibacterial packaging technologies that are able to meet these challenges. Furthermore, consumers expect and demand sustainable packaging materials that would reduce environmental problems associated with plastic waste. In this review, we discuss antimicrobial composite materials for active food packaging applications that combine highly efficient antibacterial nanoparticles (i.e., metal, metal oxide, mesoporous silica and graphene\uffe2\uff80\uff90based nanomaterials) with biodegradable and environmentally friendly green polymers (i.e., gelatin, alginate, cellulose, and chitosan) obtained from plants, bacteria, and animals. In addition, innovative syntheses and processing techniques used to obtain active and safe packaging are showcased. Implementation of such green active packaging can significantly reduce the risk of foodborne pathogen outbreaks, improve food safety and quality, and minimize product losses, while reducing waste and maintaining sustainability.Microbial transformation of soil organic matter plays a critical role in carbon (C) cycling making it essential to understand how land use and management practices influence microbial physiology and its connection to C dynamics. One factor that is likely to impact soil microbial physiology is crop diversification via its influence on belowground diversity (e.g., chemical heterogeneity of C inputs, microbial community composition). However, the effect of crop diversification measures on microbial physiology and potential effects on C cycling in agricultural soils is still unclear. To address this knowledge gap, we sampled topsoil from eight experimental sites covering different crop diversification measures across Europe (i.e., cover crops, ley farming, vegetation stripes). We used the 18O\uffe2\uff80\uff90labelling method to analyse microbial C use efficiency (CUE), growth, respiration and biomass C. Additionally, a second sampling at five selected sites examined whether the growing season influenced the impact of crop diversification. Meta\uffe2\uff80\uff90analysis revealed no overall effect of crop diversification on CUE, microbial activity, biomass or soil organic C (SOC). However, the effects varied with the type of diversification measure: cover crops did not affect carbon processing, vegetation stripes increased microbial activity, and ley farming enhanced CUE. The largest variation in CUE was observed between samplings at the same sites, indicating seasonal dynamics. Temperature, precipitation and photosynthetically active radiation predicted seasonal variation in CUE (R2\uffe2\uff80\uff89=\uffe2\uff80\uff890.36). While cover crops did not significantly impact C storage in our study, both ley farming and vegetation stripes increased SOC. The overall effect of crop diversification on SOC seems to be decoupled from highly temporally variable CUE in the bulk soil and rather relate to C\uffe2\uff80\uff90inputs.The response of terrestrial ecosystems to rising atmospheric CO2 concentration (Ca), particularly under nutrient\uffe2\uff80\uff90limited conditions, is a major uncertainty in Earth System models. The Eucalyptus Free\uffe2\uff80\uff90Air CO2 Enrichment (EucFACE) experiment, recently established in a nutrient\uffe2\uff80\uff90 and water\uffe2\uff80\uff90limited woodland presents a unique opportunity to address this uncertainty, but can best do so if key model uncertainties have been identified in advance. We applied seven vegetation models, which have previously been comprehensively assessed against earlier forest FACE experiments, to simulate a priori possible outcomes from EucFACE. Our goals were to provide quantitative projections against which to evaluate data as they are collected, and to identify key measurements that should be made in the experiment to allow discrimination among alternative model assumptions in a postexperiment model intercomparison. Simulated responses of annual net primary productivity (NPP) to elevated Ca ranged from 0.5 to 25% across models. The simulated reduction of NPP during a low\uffe2\uff80\uff90rainfall year also varied widely, from 24 to 70%. Key processes where assumptions caused disagreement among models included nutrient limitations to growth; feedbacks to nutrient uptake; autotrophic respiration; and the impact of low soil moisture availability on plant processes. Knowledge of the causes of variation among models is now guiding data collection in the experiment, with the expectation that the experimental data can optimally inform future model improvements.", "keywords": ["[SDE] Environmental Sciences", "550", "[SDV]Life Sciences [q-bio]", "Climate Change", "ecosystem model", "drought", "Forests", "551", "01 natural sciences", "Carbon Cycle", "XXXXXX - Unknown", "phosphorus", "Photosynthesis", "Ecosystem", "0105 earth and related environmental sciences", "580", "2. Zero hunger", "Eucalyptus", "droughts", "carbon dioxide", "Water", "Carbon Dioxide", "15. Life on land", "Eucalyptus tereticornis", "[SDV] Life Sciences [q-bio]", "13. Climate action", "[SDE]Environmental Sciences", "ecosystems"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.13268"}, {"href": "https://doi.org/10.1111/gcb.13268"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.13268", "name": "item", "description": "10.1111/gcb.13268", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.13268"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-05-09T00:00:00Z"}}, {"id": "10.1111/gcb.14139", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:21Z", "type": "Journal Article", "created": "2018-03-23", "title": "Elevated CO 2 did not affect the hydrological balance of a mature native Eucalyptus woodland", "description": "AbstractElevated atmospheric CO2 concentration (eCa) might reduce forest water\uffe2\uff80\uff90use, due to decreased transpiration, following partial stomatal closure, thus enhancing water\uffe2\uff80\uff90use efficiency and productivity at low water availability. If evapotranspiration (Et) is reduced, it may subsequently increase soil water storage (\uffce\uff94S) or surface runoff (R) and drainage (Dg), although these could be offset or even reversed by changes in vegetation structure, mainly increased leaf area index (L). To understand the effect of eCa in a water\uffe2\uff80\uff90limited ecosystem, we tested whether 2\uffc2\uffa0years of eCa (~40% increase) affected the hydrological partitioning in a mature water\uffe2\uff80\uff90limited Eucalyptus woodland exposed to Free\uffe2\uff80\uff90Air CO2 Enrichment (FACE). This timeframe allowed us to evaluate whether physiological effects of eCa reduced stand water\uffe2\uff80\uff90use irrespective of L, which was unaffected by eCa in this timeframe. We hypothesized that eCa would reduce tree\uffe2\uff80\uff90canopy transpiration (Etree), but excess water from reduced Etree would be lost via increased soil evaporation and understory transpiration (Efloor) with no increase in \uffce\uff94S, R or Dg. We computed Et, \uffce\uff94S, R and Dg from measurements of sapflow velocity, L, soil water content (\uffce\uffb8), understory micrometeorology, throughfall and stemflow. We found that eCa did not affect Etree, Efloor, \uffce\uff94S or \uffce\uffb8 at any depth (to 4.5\uffc2\uffa0m) over the experimental period. We closed the water balance for dry seasons with no differences in the partitioning to R and Dg between Ca levels. Soil temperature and \uffce\uffb8 were the main drivers of Efloor while vapour pressure deficit\uffe2\uff80\uff90controlled Etree, though eCa did not significantly affect any of these relationships. Our results suggest that in the short\uffe2\uff80\uff90term, eCa does not significantly affect ecosystem water\uffe2\uff80\uff90use at this site. We conclude that water\uffe2\uff80\uff90savings under eCa mediated by either direct effects on plant transpiration or by indirect effects via changes in L or soil moisture availability are unlikely in water\uffe2\uff80\uff90limited mature eucalypt woodlands.
", "keywords": ["plant-water relationships", "[SDE] Environmental Sciences", "0106 biological sciences", "0301 basic medicine", "Vapor Pressure", "[SDV]Life Sciences [q-bio]", "interception", "Forests", "01 natural sciences", "free-air CO2 enrichment", "Soil", "03 medical and health sciences", "XXXXXX - Unknown", "water-use efficiency", "0105 earth and related environmental sciences", "580", "tree water", "Eucalyptus", "Temperature", "carbon dioxide", "Water", "Plant Transpiration", "Carbon Dioxide", "15. Life on land", "Eucalyptus tereticornis", "6. Clean water", "[SDV] Life Sciences [q-bio]", "Plant Leaves", "climate change", "stomatal conductance", "13. Climate action", "[SDE]Environmental Sciences", "Seasons", "Hydrology"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.14139"}, {"href": "https://doi.org/10.1111/gcb.14139"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.14139", "name": "item", "description": "10.1111/gcb.14139", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.14139"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-04-17T00:00:00Z"}}, {"id": "10.1111/gcb.14440", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:21Z", "type": "Journal Article", "created": "2018-09-22", "title": "Cascading effects from plants to soil microorganisms explain how plant species richness and simulated climate change affect soil multifunctionality", "description": "AbstractDespite their importance, how plant communities and soil microorganisms interact to determine the capacity of ecosystems to provide multiple functions simultaneously (multifunctionality) under climate change is poorly known. We conducted a common garden experiment using grassland species to evaluate how plant functional structure and soil microbial (bacteria and protists) diversity and abundance regulate soil multifunctionality responses to joint changes in plant species richness (one, three and six species) and simulated climate change (3\uffc2\uffb0C warming and 35% rainfall reduction). The effects of species richness and climate on soil multifunctionality were indirectly driven via changes in plant functional structure and their relationships with the abundance and diversity of soil bacteria and protists. More specifically, warming selected for the larger and most productive plant species, increasing the average size within communities and leading to reductions in functional plant diversity. These changes increased the total abundance of bacteria that, in turn, increased that of protists, ultimately promoting soil multifunctionality. Our work suggests that cascading effects between plant functional traits and the abundance of multitrophic soil organisms largely regulate the response of soil multifunctionality to simulated climate change, and ultimately provides novel experimental insights into the mechanisms underlying the effects of biodiversity and climate change on ecosystem functioning.There is growing international interest in better managing soils to increase soil organic carbon (SOC) content to contribute to climate change mitigation, to enhance resilience to climate change and to underpin food security, through initiatives such as international \uffe2\uff80\uff984p1000\uffe2\uff80\uff99 initiative and the FAO's Global assessment of SOC sequestration potential (GSOCseq) programme. Since SOC content of soils cannot be easily measured, a key barrier to implementing programmes to increase SOC at large scale, is the need for credible and reliable measurement/monitoring, reporting and verification (MRV) platforms, both for national reporting and for emissions trading. Without such platforms, investments could be considered risky. In this paper, we review methods and challenges of measuring SOC change directly in soils, before examining some recent novel developments that show promise for quantifying SOC. We describe how repeat soil surveys are used to estimate changes in SOC over time, and how long\uffe2\uff80\uff90term experiments and space\uffe2\uff80\uff90for\uffe2\uff80\uff90time substitution sites can serve as sources of knowledge and can be used to test models, and as potential benchmark sites in global frameworks to estimate SOC change. We briefly consider models that can be used to simulate and project change in SOC and examine the MRV platforms for SOC change already in use in various countries/regions. In the final section, we bring together the various components described in this review, to describe a new vision for a global framework for MRV of SOC change, to support national and international initiatives seeking to effect change in the way we manage our soils.
", "keywords": ["[SDE] Environmental Sciences", "550", "BULK-DENSITY", "QH301 Biology", "Climate", "[SDV]Life Sciences [q-bio]", "NEW-ZEALAND", "630", "Soil", "NE/M021327/1", "11. Sustainability", "SDG 13 - Climate Action", "AGRICULTURAL SOILS", "SDG 15 - Life on Land", "General Environmental Science", "agriculture", "2. Zero hunger", "Global and Planetary Change", "reporting", "Measurement", "Ecology", "IN-SITU", "Agricultura", "NE/P019455/1", "carbono org\u00e1nico del suelo", "Agriculture", "LAND-USE CHANGE", "04 agricultural and veterinary sciences", "[SDV] Life Sciences [q-bio]", "climate change", "Sustainability", "[SDE]Environmental Sciences", "Carbon Sequestration", "DIFFUSE-REFLECTANCE SPECTROSCOPY", "LONG-TERM EXPERIMENTS", "330", "Monitoring", "STOCK CHANGES", "MRV", "secuestro de carbon", "12. Responsible consumption", "QH301", "Greenhouse Gases", "ORGANIC-CARBON", "soil organic matter", "greenhouse gases", "Invited Research Reviews", "Environmental Chemistry", "774378", "SDG 2 - Zero Hunger", "European Commission", "resilience", "Climate Solutions", "Soil organic matter", "Soil organic carbon", "Natural Environment Research Council (NERC)", "Verification", "food security", "15. Life on land", "carbon sequestration", "Sustainable Agriculture", "Carbon", "EDDY-COVARIANCE", "soil organic carbon", "monitoring", "Reporting", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "measurement", "verification"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.14815"}, {"href": "https://scholarworks.uvm.edu/context/rsfac/article/1079/viewcontent/Lini2019b.pdf"}, {"href": "https://doi.org/10.1111/gcb.14815"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.14815", "name": "item", "description": "10.1111/gcb.14815", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.14815"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-10-06T00:00:00Z"}}, {"id": "10.1111/j.1365-2389.1990.tb00228.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:26Z", "type": "Journal Article", "created": "2006-07-29", "title": "Effect Of Tillage On Soil Organic Carbon Mineralization Estimated From 13c Abundance In Maize Fields", "description": "SUMMARYThree methods of cultivation, conventional tillage (CT), superficial tillage (ST) and no\uffe2\uff80\uff90tillage (NT), were applied for 17 years to continuous maize. Their effect on soil organic carbon content was investigated through measurements of carbon and 13C/12C ratios, using the natural difference in 13C content between C3 plants and maize, which is a C4 plant. Because the soil had carried C3 plants before the experiment started, the organic carbon remaining from that time (C3,\uffe2\uff80\uff90carbon), was distinguished from the carbon derived from maize. Comparison between continuous wheat and maize plots showed that organic matter from both maize and wheat decomposed without significant 13C enrichment, whereas older C3\uffe2\uff80\uff90carbon was enriched by 1.5% compared to that of fresh wheat material. From the initial 3.6 kg C m\uffe2\uff88\uff922 in the topsoil (0\uffe2\uff80\uff9330 cm), 0.95 were mineralized in the CT treatment, but only 0.45 in NT. The mineralization was the same in the tilled layer of ST as in CT. The CT treatment accumulated 1.1 kg C m\uffe2\uff88\uff922 of maize\uffe2\uff80\uff90derived carbon and the NT treatment 0.8. The mineralization of initial C3\uffe2\uff80\uff90carbon was the same at all depths between 0 and 30 cm in the NT treatment; 75% of the carbon derived from maize was found in the 0\uffe2\uff80\uff935 cm layer.
", "keywords": ["[SDE] Environmental Sciences", "2. Zero hunger", "ma\u00efs", "[SDV]Life Sciences [q-bio]", "04 agricultural and veterinary sciences", "15. Life on land", "carbone 13", "zea mays", "[SDV] Life Sciences [q-bio]", "marqueur isotopique", "[SDE]Environmental Sciences", "0401 agriculture", " forestry", " and fisheries", "min\u00e9ralisation", "syst\u00e8me de culture"], "contacts": [{"organization": "Balesdent, J\u00e9r\u00f4me, Mariotti, Andr\u00e9, Boisgontier, D.,", "roles": ["creator"]}]}, "links": [{"href": "https://hal.inrae.fr/hal-02700909/file/101949_20110203054704668_1.pdf"}, {"href": "https://doi.org/10.1111/j.1365-2389.1990.tb00228.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Soil%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2389.1990.tb00228.x", "name": "item", "description": "10.1111/j.1365-2389.1990.tb00228.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2389.1990.tb00228.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1990-12-01T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2007.01439.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:30Z", "type": "Journal Article", "created": "2007-10-18", "title": "Co2balance Of Boreal, Temperate, And Tropical Forests Derived From A Global Database", "description": "AbstractTerrestrial ecosystems sequester 2.1\uffe2\uff80\uff83Pg of atmospheric carbon annually. A large amount of the terrestrial sink is realized by forests. However, considerable uncertainties remain regarding the fate of this carbon over both short and long timescales. Relevant data to address these uncertainties are being collected at many sites around the world, but syntheses of these data are still sparse. To facilitate future synthesis activities, we have assembled a comprehensive global database for forest ecosystems, which includes carbon budget variables (fluxes and stocks), ecosystem traits (e.g. leaf area index, age), as well as ancillary site information such as management regime, climate, and soil characteristics. This publicly available database can be used to quantify global, regional or biome\uffe2\uff80\uff90specific carbon budgets; to re\uffe2\uff80\uff90examine established relationships; to test emerging hypotheses about ecosystem functioning [e.g. a constant net ecosystem production (NEP) to gross primary production (GPP) ratio]; and as benchmarks for model evaluations. In this paper, we present the first analysis of this database. We discuss the climatic influences on GPP, net primary production (NPP) and NEP and present the CO2 balances for boreal, temperate, and tropical forest biomes based on micrometeorological, ecophysiological, and biometric flux and inventory estimates. Globally, GPP of forests benefited from higher temperatures and precipitation whereas NPP saturated above either a threshold of 1500\uffe2\uff80\uff83mm precipitation or a mean annual temperature of 10 \uffc2\uffb0C. The global pattern in NEP was insensitive to climate and is hypothesized to be mainly determined by nonclimatic conditions such as successional stage, management, site history, and site disturbance. In all biomes, closing the CO2 balance required the introduction of substantial biome\uffe2\uff80\uff90specific closure terms. Nonclosure was taken as an indication that respiratory processes, advection, and non\uffe2\uff80\uff90CO2 carbon fluxes are not presently being adequately accounted for.
", "keywords": ["0106 biological sciences", "environment/Bioclimatology", "550", "[SDV]Life Sciences [q-bio]", "01 natural sciences", "630", "SDG 17 - Partnerships for the Goals", "carbon cycle", "SDG 13 - Climate Action", "carbon cycle; forest ecosystems; global database; gross primary productivity; net ecosystem productivity; net primary productivity", "net primary productivity", "global database", "0105 earth and related environmental sciences", "Ecology", "net ecosystem productivity", "forest ecosystems", "Biological Sciences", "15. Life on land", "Climate Action", "[SDV] Life Sciences [q-bio]", "[SDV.EE.BIO] Life Sciences [q-bio]/Ecology", " environment/Bioclimatology", "13. Climate action", "[SDV.EE.BIO]Life Sciences [q-bio]/Ecology", "CO2", "gross primary productivity", "Environmental Sciences"]}, "links": [{"href": "https://escholarship.org/content/qt57t1t77c/qt57t1t77c.pdf"}, {"href": "https://doi.org/10.1111/j.1365-2486.2007.01439.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2007.01439.x", "name": "item", "description": "10.1111/j.1365-2486.2007.01439.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2007.01439.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-08-21T00:00:00Z"}}, {"id": "10.1111/ppl.13738", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:46Z", "type": "Journal Article", "created": "2022-06-29", "title": "Gypsum endemics accumulate excess nutrients in leaves as a potential constitutive strategy to grow in grazed extreme soils", "description": "AbstractExtreme soils often have mineral nutrient imbalances compared to plant nutritional requirements and co\uffe2\uff80\uff90occur in open areas where grazers thrive. Thus, plants must respond to both constraints, which can affect nutrient concentrations in all plant organs. Gypsum soil provides an excellent model system to study adaptations to extreme soils under current grazing practices as it harbours two groups of plant species that differ in their tolerance to gypsum soils and foliar composition. However, nutrient concentrations in organs other than leaves, and their individual responses to simulated herbivory, are still unknown in gypsum plants. We studied plant biomass, root mass ratio and nutrient partitioning among different organs (leaves, stems, coarse roots, fine roots) in five gypsum endemics and five generalists cultivated in gypsum and calcareous soils and subjected to different levels of simulated browsing. Gypsum endemics tended to have higher elemental concentration in leaves, stems and coarse roots than generalist species in both soil types, whereas both groups tended to show similar high concentrations in fine roots. This behaviour was especially clear with sulphur (S), which is found in excess in gypsum soils, and which endemics accumulated in leaves as sulphate (>50% of S). Moreover, plants subjected to clipping, regardless of their affinity to gypsum, were unable to compensate for biomass losses and showed similar elemental composition to unclipped plants. The accumulation of excess mineral nutrients by endemic species in aboveground organs may be a constitutive nutritional strategy in extreme soils and is potentially playing an anti\uffe2\uff80\uff90herbivore role in grazed gypsum outcrops. Mid\uffe2\uff80\uff90term (MTEs, 5\uffe2\uff80\uff9320\uffe2\uff80\uff89years) and long\uffe2\uff80\uff90term (LTEs, 20+\uffe2\uff80\uff89years) field experiments are key sources of information to design future climate\uffe2\uff80\uff90smart agriculture. Within the European Joint Program SOIL (EJP SOIL), we built the EJP SOIL\uffe2\uff80\uff90MTE/LTE metadataset that contains metadata from 240 MTEs/LTEs across Europe. Metadata collected included precise descriptions of the treatments (combination of factors such as tillage, crop type/rotation, amendments/fertilizers, grazing and pest/weed management), soil and crop measurements and pedo\uffe2\uff80\uff90climatic information. Using different figures and dashboards, an overview of those MTEs/LTEs is presented and specific research themes (tillage systems, residue management, amendment type and cover crops) are further analysed within their pedo\uffe2\uff80\uff90climatic context. An interactive web portal developed in collaboration with the BonaRes project ( https://lte.bonares.de ), enables users to explore the metadataset and find relevant MTEs/LTEs for specific combinations of practices (e.g. all MTEs/LTEs that investigate cover crops on a Cambisol in no\uffe2\uff80\uff90tillage system). Finally, a SWOT (Strengths, Weaknesses, Opportunities, Threats) analysis of the metadataset was carried out to highlight the potential contribution of MTEs/LTEs to a harmonized European soil observation and monitoring approach. We propose that the metadataset could be elaborated with metadata from other existing MTEs/LTEs in Europe or even worldwide. 1.\uffe2\uff80\uff82Depending on grazing intensity, grasslands tend towards two contrasting systems that differ in terms of species diversity and soil carbon (C) storage. To date, effects of grazing on C cycling have mainly been studied in grasslands subject to constant grazing regimes, whereas little is known for grasslands experiencing a change in grazing intensity. Analysing the transition between C\uffe2\uff80\uff90storing and C\uffe2\uff80\uff90releasing grasslands under low\uffe2\uff80\uff90 and high\uffe2\uff80\uff90grazing regimes, respectively, will help to identify key plant\uffe2\uff80\uff93soil interactions for C cycling.
2.\uffe2\uff80\uff82The transition was studied in a mesocosm experiment with grassland monoliths submitted to a change in grazing after 14\uffe2\uff80\uff83years of constant high and low grazing. Plant\uffe2\uff80\uff93soil interactions were analysed by following the dynamics of plant and microbial communities, roots and soil organic matter fractions over 2\uffe2\uff80\uff83years. After disturbance change, mesocosms were continuously exposed to13C\uffe2\uff80\uff90labelled CO2, which allowed us to trace both the incorporation of new litter C produced by a modified plant community in soil and the fate of old unlabelled litter C.
3.\uffe2\uff80\uff82Changing disturbance intensity led to a cascade of events. After shift to high disturbance, photosynthesis decreased followed by a decline in root biomass and a change in plant community structure 1.5\uffe2\uff80\uff83months later. Those changes led to a decrease of soil fungi, a proliferation of Gram(+) bacteria and accelerated decomposition of old particulate organic C (<6\uffe2\uff80\uff83months). At last, accelerated decomposition released plant available nitrogen and decreased soil C storage. Our results indicate that intensified grazing triggers proliferation of Gram(+) bacteria and subsequent faster decomposition by reducing roots adapted to low disturbance.
4.\uffe2\uff80\uff82Synthesis. Plant communities exert control on microbial communities and decomposition through the activity of their living roots: slow\uffe2\uff80\uff90growing plants adapted to low disturbance reduce Gram(+) bacteria, decomposition of low and high quality litter, nitrogen availability and, thus, ingress of fast\uffe2\uff80\uff90growing plants. Our results indicate that grazing impacts on soil carbon storage by altering plant roots and their control on the soil microbial community and decomposition, and that these processes will foster decomposition and soil C loss in more productive and disturbed grassland systems.
", "keywords": ["580", "disturbance", "[SDE] Environmental Sciences", "2. Zero hunger", "decomposition", "[SDV]Life Sciences [q-bio]", "carbon cycling", "04 agricultural and veterinary sciences", "15. Life on land", "matter", "[SDV] Life Sciences [q-bio]", "[SDV.EE] Life Sciences [q-bio]/Ecology", " environment", "nitrogen cycling", "13. Climate action", "[SDV.EE]Life Sciences [q-bio]/Ecology", "ARISA", "[SDE]Environmental Sciences", "PLFA", "0401 agriculture", " forestry", " and fisheries", "grassland", "microbial community", "environment", "management", "particulate organic"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2745.2009.01549.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2745.2009.01549.x", "name": "item", "description": "10.1111/j.1365-2745.2009.01549.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2745.2009.01549.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-08-11T00:00:00Z"}}, {"id": "10.1111/j.1475-2743.2010.00314.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:39Z", "type": "Journal Article", "created": "2010-11-23", "title": "Response Of Organic Matter To Reduced Tillage And Animal Manure In A Temperate Loamy Soil", "description": "AbstractThe impacts of tillage and organic fertilization on soil organic matter (SOM) are highly variable and still unpredictable, and their interactions need to be investigated under various soil, climate and cropping system conditions. Our work examined the effect of reduced tillage and animal manure on SOM stocks and quality in the 0\uffe2\uff80\uff9340\uffe2\uff80\uff83cm layer of a loamy soil under mixed cropping system and humid temperate climate. The soil organic carbon (SOC) and N stocks, particulate organic matter (POM), and C and N mineralization potential (301\uffe2\uff80\uff83days at 15\uffe2\uff80\uff83\uffc2\uffb0C) were measured in a 8\uffe2\uff80\uff90yr\uffe2\uff80\uff90old split\uffe2\uff80\uff90plot field trial, including three tillage treatments [mouldboard ploughing (MP), shallow tillage (ST), no tillage (NT)] and two fertilization treatments [mineral (M), poultry manure 2.2\uffe2\uff80\uff83t/ha/yr C (O)]. No statistically significant interactive effects of tillage and fertilization were measured except on C mineralization. NT and ST showed greater SOC stocks (41.2 and 39.7\uffe2\uff80\uff83t/ha C) than MP (37.1\uffe2\uff80\uff83t/ha C) in the 0\uffe2\uff80\uff9315\uffe2\uff80\uff83cm increment, while no statistical differences were observed at a greater depth. N stocks exhibited similar distribution patterns with regard to tillage effect. Animal manure, applied at a rate representative of typical field application rates, had a smaller impact on SOC and N stocks than tillage. The mean SOC and N stocks were higher under O than M, but the differences were statistically significant only in the 0\uffe2\uff80\uff935\uffe2\uff80\uff83cm increment. MP showed lower C\uffe2\uff80\uff90POM stocks than NT and ST in the 0\uffe2\uff80\uff935\uffe2\uff80\uff83cm increment, whereas greater C\uffe2\uff80\uff90POM stocks were measured under MP than under NT or under ST in the 20\uffe2\uff80\uff9325\uffe2\uff80\uff83cm increment. Organic fertilization had no impact on C\uffe2\uff80\uff90POM or N\uffe2\uff80\uff90POM stocks. In the 0\uffe2\uff80\uff9325\uffe2\uff80\uff83cm increment, NT showed a lower C and N mineralization potential than MP. Our work shows that the sensitivity of SOM to reduced tillage for the whole soil profile can be relatively small in a loamy soil, under humid\uffe2\uff80\uff90temperate climate. However, POM was particularly sensitive to the differential effects of tillage practices with depth, and indicative of differentiation in total SOM distribution in the soil profile.
", "keywords": ["Carbon sequestration", "[SDE] Environmental Sciences", "2. Zero hunger", "[SDV]Life Sciences [q-bio]", "04 agricultural and veterinary sciences", "shallow tillage", "[INFO] Computer Science [cs]", "15. Life on land", "630", "6. Clean water", "[SDV] Life Sciences [q-bio]", "no tillage", "[SDE]Environmental Sciences", "0401 agriculture", " forestry", " and fisheries", "[INFO]Computer Science [cs]", "organic amendment"]}, "links": [{"href": "https://doi.org/10.1111/j.1475-2743.2010.00314.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Use%20and%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1475-2743.2010.00314.x", "name": "item", "description": "10.1111/j.1475-2743.2010.00314.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1475-2743.2010.00314.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-11-23T00:00:00Z"}}, {"id": "10.1111/j.1529-8817.2003.00746.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:40Z", "type": "Journal Article", "created": "2004-03-30", "title": "Atmospheric Co2 Elevation Has Little Effect On Nitrifying And Denitrifying Enzyme Activity In Four European Grasslands", "description": "AbstractThe objective of this study was to determine what patterns, if any, existed in the response of nitrifying enzyme activity (NEA), denitrifying enzyme activity (DEA), soil microbial N and soil inorganic N to elevated CO2 across a broad range of grassland environments. We studied the response of these N pools and microbial activities in four CO2\uffe2\uff80\uff90enrichment sites of the MEGARICH project (Managing European Grasslands as a Sustainable Resource in a Changing Climate). CO2 treatment was studied in factorial combination with a cutting frequency treatment at two sites and with a temperature treatment at one site. Our study showed that microbial biomass N, NEA, DEA and extractable soil [NH4+] and [NO3\uffe2\uff88\uff92] were generally not affected by elevated CO2 in these grassland ecosystems after several years of treatment, nor by cutting frequency or temperature at the sites that included these treatments. Exceptions to this were that DEA and soil [NO3\uffe2\uff88\uff92] decreased by 22% and 45%, respectively, at the French site at elevated CO2. We discuss the possible explanations for this lack of response.
", "keywords": ["580", "2. Zero hunger", "RAY GRASS ANGLAIS", "[SDV]Life Sciences [q-bio]", "MEGARICH PROJECT", "AMMONIUM", "04 agricultural and veterinary sciences", "15. Life on land", "ammonium", "[SDV] Life Sciences [q-bio]", "IMMOBILIZATION", "nitrate", "13. Climate action", "immobilization", "0401 agriculture", " forestry", " and fisheries", "GLOBAL CHANGE", "MEGARICH project", "global change", "NITRATE"]}, "links": [{"href": "https://doi.org/10.1111/j.1529-8817.2003.00746.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1529-8817.2003.00746.x", "name": "item", "description": "10.1111/j.1529-8817.2003.00746.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1529-8817.2003.00746.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-02-19T00:00:00Z"}}, {"id": "10.1111/j.1747-0765.2008.00330.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:41Z", "type": "Journal Article", "created": "2009-01-29", "title": "Salinity And Organic Amendment Effects On Methane Emission From A Rain-Fed Saline Paddy Field", "description": "Abstract Organic amendment is a traditional practice for rehabilitating saline patches in north-east Thailand, but organic matter is known to enhance methane emission. However, a high degree of salinity might mitigate methane emission. The objective of the present study was to quantify the effects of salinity and organic amendments on methane emission from rain-fed paddy fields exposed to increasing salinity. A paddy field on a salt-affected Typic Natraqualfs was selected. Eighteen experimental plots were located in two randomized complete block designs placed inside and outside a saline patch. Each design of nine plots presented three replicates of two different organic amendments and a control treatment without organic amendment. During the rainy season the soil electrical conductivity, measured with an electromagnetic conductivity meter (ECEM), was greater than 300\u00a0mS\u00a0m\u22121 inside the saline patch, whereas outside the saline patch the values were lower than 200\u00a0mS\u00a0m\u22121. Rice straw (6.25\u00a0t\u00a0ha\u22121) and cow ma...", "keywords": ["[SDE] Environmental Sciences", "soil salinity", "550", "[SDV]Life Sciences [q-bio]", "north-east Thailand", "methane emission", "NORTH-EAST THAILAND", "saline paddy", "SOIL SALINITY", "630", "soil", "METHANE", "METHANE EMISSION", "ORGANIC AMENDMENTS", "2. Zero hunger", "SALINE PADDY SOIL", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "[SDV] Life Sciences [q-bio]", "organic amendments", "13. Climate action", "[SDE]Environmental Sciences", "0401 agriculture", " forestry", " and fisheries", "RIZ"]}, "links": [{"href": "https://doi.org/10.1111/j.1747-0765.2008.00330.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Science%20and%20Plant%20Nutrition", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1747-0765.2008.00330.x", "name": "item", "description": "10.1111/j.1747-0765.2008.00330.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1747-0765.2008.00330.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-02-01T00:00:00Z"}}, {"id": "10.3389/fenvs.2018.00061", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:21:23Z", "type": "Journal Article", "created": "2018-07-10", "title": "Recognizing Patterns: Spatial Analysis of Observed Microbial Colonization on Root Surfaces", "description": "Root surfaces are major sites of interactions between plants and associated microorganisms. Here, plants and microbes communicate via signaling molecules, compete for nutrients, and release substrates that may have beneficial or harmful effects on each other. Whilst the body of knowledge on the abundance and diversity of microbial communities at root-soil interfaces is now substantial, information on their spatial distribution at the microscale is still scarce. In this study, a standardized method for recognizing and analyzing microbial cell distributions on root surfaces is presented. Fluorescence microscopy was combined with automated image analysis and spatial statistics to explore the distribution of bacterial colonization patterns on rhizoplanes of rice roots. To test and evaluate the presented approach, a gnotobiotic experiment was performed using a potential nitrogen-fixing bacterial strain in combination with roots of wetland rice. The automated analysis procedure resulted in reliable spatial data of bacterial cells colonizing the rhizoplane. Among all replicate roots, the analysis revealed an increasing density of bacterial cells from the root tip to the region of root cell maturation. Moreover, bacterial cells showed significant spatial clustering and tended to be located around plant root cell borders. The quantitative data suggest that the structure of the root surface plays a major role in bacterial colonization patterns. Possible adaptations of the presented approach for future studies are discussed along with potential pitfalls such as inaccurate imaging. Our results demonstrate that standardized recognition and statistical evaluation of microbial colonization on root surfaces holds the potential to increase our understanding of microbial associations with roots and of the underlying ecological interactions.", "keywords": ["[SDE] Environmental Sciences", "0301 basic medicine", "570", "bacterial colonization", "[SDV]Life Sciences [q-bio]", "CATALYZED REPORTER DEPOSITION", "microbial ecology;root surface;bacterial colonization;point process;spatial statistics;image analysis;pattern recognition;wetland rice", "ECOLOGY", "microbial ecology", "Image analysis", "spatial statistics", "Microbial ecology", "03 medical and health sciences", "image analysis", "Pattern recognition", "root surface", "GE1-350", "Point process", "Wetland rice", "point process", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "Spatial statistics", "IDENTIFICATION", "pattern recognition", "IN-SITU HYBRIDIZATION", "15. Life on land", "Bacterial colonization", "[SDV] Life Sciences [q-bio]", "SOIL", "Environmental sciences", "wetland rice", "Root surface", "[SDE]Environmental Sciences", "BACTERIA", "106022 Microbiology", "POPULATIONS", "COMMUNITIES"]}, "links": [{"href": "https://doi.org/10.3389/fenvs.2018.00061"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Environmental%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fenvs.2018.00061", "name": "item", "description": "10.3389/fenvs.2018.00061", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fenvs.2018.00061"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-10T00:00:00Z"}}, {"id": "10.1139/as-2022-0006", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:51Z", "type": "Journal Article", "created": "2022-07-04", "title": "Monitoring guidelines for polymer identification, quality assurance/quality control (QA/QC) and data reporting for monitoring of microplastics in the Arctic environment", "description": "The pollution of the environment with plastics is of growing concern worldwide, including the Arctic region. While larger plastic pieces are a visible pollution issue, smaller microplastics are not visible with the naked eye. These particles are available for interaction by Arctic biota and have become a concern for animal and human health. The determination of microplastic properties includes several methodological steps, i.e., sampling, extraction, quantification, and chemical identification. This review discusses suitable analytical tools for the identification, quantification, and characterization of microplastics in the context of monitoring in the Arctic. It further addresses quality assurance and quality control (QA/QC), which is particularly important for the determination of microplastic in the Arctic, as both contamination and analyte losses can occur. It presents specific QA/QC measures for sampling procedures and for the handling of samples in the laboratory, either on land or on ship, and considering the small size of microplastics as well as the high risk of contamination. The review depicts which data should be mandatory to report, thereby supporting a framework for harmonized data reporting.
", "keywords": [":Analytisk kjemi: 445 [VDP]", "0211 other engineering and technologies", "Environmental engineering", "QA/QC", "02 engineering and technology", "Massespektrografi", "01 natural sciences", "[SDU] Sciences of the Universe [physics]", ":Analytical chemistry: 445 [VDP]", "Arctic", "VDP::Analytical chemistry: 445", "GE1-350", "14. Life underwater", "QA", "Raman", "QC", "0105 earth and related environmental sciences", "reporting", "Mass spectrometry", "TED-GC/MS", "TED-GC", "py-GC/MS", "Microplastic", "py-GC", "Fourier transform infrared spectroscopy", "MS", "VDP::Analytisk kjemi: 445", "TA170-171", "Microplast", "620", "Environmental sciences", "[SDV] Life Sciences [q-bio]", "monitoring", "FTIR", "13. Climate action", "microscopy", "microplastic"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/536963/1/primpke-et-al-2022-monitoring-of-microplastic-pollution-in-the-arctic-recent-developments-in-polymer-identification.pdf"}, {"href": "https://cdnsciencepub.com/doi/pdf/10.1139/as-2022-0006"}, {"href": "https://doi.org/10.1139/as-2022-0006"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Arctic%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1139/as-2022-0006", "name": "item", "description": "10.1139/as-2022-0006", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1139/as-2022-0006"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-03-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=%5BSDV%5D+Life+Sciences+%5Bq-bio%5D&offset=50&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=%5BSDV%5D+Life+Sciences+%5Bq-bio%5D&offset=50&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "prev", "title": "items (prev)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=%5BSDV%5D+Life+Sciences+%5Bq-bio%5D&offset=0", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=%5BSDV%5D+Life+Sciences+%5Bq-bio%5D&offset=100", "hreflang": "en-US"}], "numberMatched": 160, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-06-25T14:30:55.456512Z"}