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This work reviewed seven important European LCA studies in a sensitivity analysis approach in order to get a better understanding of the roots of such a debate for three major biofuels in European production: rape methyl ester and ethanol from wheat and sugar beet. Global trends and variability of energy and GHG balances were depicted and completed with a sensitivity analysis carried out for each methodological and data parameter, which allowed making recommendations on the carrying out of LCA in a policy-making or a biofuels comparison context. Methodological choices, and especially allocation rule, appeared as key elements for results variation with influences on balances up to 149%; system expansion approach was identified as the most relevant rule since it integrates the market potential and the environmental interest of by-products promotion, which was pointed out as a crucial point for biofuels sustainability. The influence of local specificity for cultivation data was evaluated up to 167%, which puts too large geographical coverage in question. Modelling uncertainties due to N2O emissions from soils showed influences from 17 to 46%, which represents a crucial challenge for research and for LCA results accuracy. Approximations evaluation pointed out the need to integrate agricultural machinery into the assessment. Finally, land-use issue revealed its dramatic importance for LCA results and the need to define explicit scenarios for land-use alternatives.", "keywords": ["[SDV.BIO]Life Sciences [q-bio]/Biotechnology", "330", "http://aims.fao.org/aos/agrovoc/c_24420", "P06 - Sources d'\u00e9nergie renouvelable", "http://aims.fao.org/aos/agrovoc/c_37938", "http://aims.fao.org/aos/agrovoc/c_890", "\u00e9thanol", "02 engineering and technology", "7. Clean energy", "01 natural sciences", "630", "12. Responsible consumption", "11. 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A Review", "description": "Biofuels are fuels produced from biomass, mostly in liquid form, within a time frame sufficiently short to consider that their feedstock (biomass) can be renewed, contrarily to fossil fuels. This paper reviews the current and future biofuel technologies, and their development impacts (including on the climate) within given policy and economic frameworks. Current technologies make it possible to provide first generation biodiesel, ethanol or biogas to the transport sector to be blended with fossil fuels. Still under-development 2nd generation biofuels from lignocellulose should be available on the market by 2020. Research is active on the improvement of their conversion efficiency. A ten-fold increase compared with current cost-effective capacities would make them highly competitive. Within bioenergy policies, emphasis has been put on biofuels for transportation as this sector is fast-growing and represents a major source of anthropogenic greenhouse gas emissions. Compared with fossil fuels, biofuel combustion can emit less greenhouse gases throughout their life cycle, considering that part of the emitted CO2 returns to the atmosphere where it was fixed from by photosynthesis in the first place. Life cycle assessment (LCA) is commonly used to assess the potential environmental impacts of biofuel chains, notably the impact on global warming. This tool, whose holistic nature is fundamental to avoid pollution trade-offs, is a standardised methodology that should make comparisons between biofuel and fossil fuel chains objective and thorough. However, it is a complex and time-consuming process, which requires lots of data, and whose methodology is still lacking harmonisation. Hence the life-cycle performances of biofuel chains vary widely in the literature. Furthermore, LCA is a site- and timeindependent tool that cannot take into account the spatial and temporal dimensions of emissions, and can hardly serve as a decision-making tool either at local or regional levels. Focusing on greenhouse gases, emission factors used in LCAs give a rough estimate of the potential average emissions on a national level. However, they do not take into account the types of crop, soil or management practices, for instance. Modelling the impact of local factors on the determinism of greenhouse gas emissions can provide better estimates for LCA on the local level, which would be the relevant scale and degree of reliability for decision-making purposes. Nevertheless, a deeper understanding of the processes involved, most notably N2O emissions, is still needed to definitely improve the accuracy of LCA. Perennial crops are a promising option for biofuels, due to their rapid and efficient use of nitrogen, and their limited farming operations. However, the main overall limiting factor to biofuel development will ultimately be land availability. Given the available land areas, population growth rate and consumption behaviours, it would be possible to reach by 2030 a global 10% biofuel share in the transport sector, contributing to lower global greenhouse gas emissions by up to 1 GtCO2 eq.year\u22121 (IEA, 2006), provided that harmonised policies ensure that sustainability criteria for the production systems are respected worldwide. Furthermore, policies should also be more integrative across sectors, so that changes in energy efficiency, the automotive sector and global consumption patterns converge towards drastic reduction of the pressure on resources. Indeed, neither biofuels nor other energy source or carriers are likely to mitigate the impacts of anthropogenic pressure on resources in a range that would compensate for this pressure growth. 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