{"type": "FeatureCollection", "features": [{"id": "2164/23373", "type": "Feature", "geometry": null, "properties": {"updated": "2026-10-06T16:22:59Z", "type": "Journal Article", "created": "2023-11-29", "title": "Connecting the multiple dimensions of global soil fungal diversity", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>How the multiple facets of soil fungal diversity vary worldwide remains virtually unknown, hindering the management of this essential species-rich group. By sequencing high-resolution DNA markers in over 4000 topsoil samples from natural and human-altered ecosystems across all continents, we illustrate the distributions and drivers of different levels of taxonomic and phylogenetic diversity of fungi and their ecological groups. We show the impact of precipitation and temperature interactions on local fungal species richness (alpha diversity) across different climates. Our findings reveal how temperature drives fungal compositional turnover (beta diversity) and phylogenetic diversity, linking them with regional species richness (gamma diversity). We integrate fungi into the principles of global biodiversity distribution and present detailed maps for biodiversity conservation and modeling of global ecological processes.</p></article>", "keywords": ["Supplementary Data", "QH301 Biology", "Diversity (politics)", "Plant Science", "Biodiversity conservation", "Fungal Diversity", "Agricultural and Biological Sciences", "Soil", "Life", "Sociology", "WATER", "Global biodiversity distribution", "Fungal diversity", "Phylogeny", "Soil Microbiology", "2. Zero hunger", "Multidisciplinary", "Earth", " Environmental", " Ecological", " and Space Sciences", "Geography", "Ecology", "soil fungal diversity", "4. Education", "SPECIES RICHNESS", "Life Sciences", "https://www.science.org/doi/suppl/10.1126/sciadv.adj8016/suppl_file/sciadv.adj8016_sm.pdf", "Biodiversity", "FOS: Sociology", "global biodiversity distribution", "sienet", "https://www.science.org/doi/suppl/10.1126/sciadv.adj8016/suppl_file/sciadv.adj8016_tables_s1_to_s13.zip", "Diversity and Evolution of Fungal Pathogens", "570", "Supplementary Information", "DNA markers", "QH301", "Sequencing high-resolution DNA", "Biochemistry", " Genetics and Molecular Biology", "monimuotoisuus", "Mycorrhizal Fungi and Plant Interactions", "Life Science", "Humans", "14. Life underwater", "General", "Global ecological processes", "Biology", "Ecosystem", "Ecology", " Evolution", " Behavior and Systematics", "global ecological processes", "Soil fungal diversity", "microbiology", "Fungi", "Water", "Cell Biology", "15. Life on land", "luonnon monimuotoisuus", "Agronomy", "biodiversiteetti", "LIFE", "ekosysteemit (ekologia)", "Evolution and Ecology of Endophyte-Grass Symbiosis", "13. Climate action", "Ecology", " evolutionary biology", "Earth and Environmental Sciences", "FOS: Biological sciences", "Anthropology", "ta1181", "biodiversity conservation", "CBCE", "Species richness"]}, "links": [{"href": "https://www.science.org/doi/epdf/10.1126/sciadv.adj8016"}, {"href": "https://iris.unica.it/bitstream/11584/447894/1/Mikryukov%20et%20al_Science%20Advances%202023.pdf"}, {"href": "https://www.science.org/doi/pdf/10.1126/sciadv.adj8016"}, {"href": "https://doi.org/2164/23373"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20Advances", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2164/23373", "name": "item", "description": "2164/23373", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2164/23373"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-01T00:00:00Z"}}, {"id": "2434/1115155", "type": "Feature", "geometry": null, "properties": {"updated": "2026-10-06T16:23:03Z", "type": "Journal Article", "created": "2024-06-12", "title": "Anthropogenic pollution may enhance natural transformation in water, favouring the spread of antibiotic resistance genes", "description": "Aquatic ecosystems are crucial in the antimicrobial resistance cycle. While intracellular DNA has been extensively studied to understand human activity's impact on antimicrobial resistance gene (ARG) dissemination, extracellular DNA is frequently overlooked. This study examines the effect of anthropogenic water pollution on microbial community diversity, the resistome, and ARG dissemination. We analyzed intracellular and extracellular DNA from wastewater treatment plant effluents and lake surface water by shotgun sequencing. We also conducted experiments to evaluate anthropogenic pollution's effect on transforming extracellular DNA (using Gfp-plasmids carrying ARGs) within a natural microbial community. Chemical analysis showed treated wastewater had higher anthropogenic pollution-related parameters than lake water. The richness of microbial community, antimicrobial resistome, and high-risk ARGs was greater in treated wastewaters than in lake waters both for intracellular and extracellular DNA. Except for the high-risk ARGs, richness was significantly higher in intracellular than in extracellular DNA. Several ARGs were associated with mobile genetic elements and located on plasmids. Furthermore, Gfp-plasmid transformation within a natural microbial community was enhanced by anthropogenic pollution levels. Our findings underscore anthropogenic pollution's pivotal role in shaping microbial communities and their antimicrobial resistome. Additionally, it may facilitate ARG dissemination through extracellular DNA plasmid uptake.", "keywords": ["Bacteria", "Antibiotic resistance", "Microbiota", "Water Pollution", "Metagenome assembled genomes", "Drug Resistance", " Microbial", "Horizontal gene transfer", "Wastewater", "extracellular DNA; antibiotic resistance; metagenome assembled genomes; transformation; horizontal gene transfer", "Transformation", "Anti-Bacterial Agents", "Lakes", "Extracellular DNA", "Genes", " Bacterial", "Drug Resistance", " Bacterial", "Water Microbiology", "Plasmids"]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/1115155/2/Sivalingam%20et%20al%202024.pdf"}, {"href": "https://doi.org/2434/1115155"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2434/1115155", "name": "item", "description": "2434/1115155", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2434/1115155"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-01T00:00:00Z"}}, {"id": "2791089561", "type": "Feature", "geometry": null, "properties": {"updated": "2026-10-06T16:23:07Z", "type": "Journal Article", "created": "2018-02-23", "title": "Bacterial Preferences for Specific Soil Particle Size Fractions Revealed by Community Analyses", "description": "Genetic fingerprinting demonstrated in previous studies that differently sized soil particle fractions (PSFs; clay, silt, and sand with particulate organic matter (POM)) harbor microbial communities that differ in structure, functional potentials and sensitivity to environmental conditions. To elucidate whether specific bacterial or archaeal taxa exhibit preference for specific PSFs, we examined the diversity of PCR-amplified 16S rRNA genes by high-throughput sequencing using total DNA extracted from three long-term fertilization variants (unfertilized, fertilized with minerals, and fertilized with animal manure) of an agricultural loamy sand soil and their PSFs. The PSFs were obtained by gentle ultrasonic dispersion, wet sieving, and centrifugation. The abundance of bacterial taxa assigned to operational taxonomic units (OTUs) differed less than 2.7% between unfractionated soil and soil based on combined PSFs. Across the three soil variants, no archaeal OTUs, but many bacterial OTUs, the latter representing 34-56% of all amplicon sequences, showed significant preferences for specific PSFs. The sand-sized fraction with POM was the preferred site for members of Bacteroidetes and Alphaproteobacteria, while Gemmatimonadales preferred coarse silt, Actinobacteria and Nitrosospira fine silt, and Planctomycetales clay. Firmicutes were depleted in the sand-sized fraction. In contrast, archaea, which represented 0.8% of all 16S rRNA gene sequences, showed only little preference for specific PSFs. We conclude that differently sized soil particles represent distinct microenvironments that support specific bacterial taxa and that these preferences could strongly contribute to the spatial heterogeneity and bacterial diversity found in soils.", "keywords": ["2. Zero hunger", "soil bacteria", "Soil bacteria", "soil DNA", "bacterial diversity", "04 agricultural and veterinary sciences", "Soil DNA", "15. Life on land", "Microbiology", "Soil archaea", "QR1-502", "Archaeal diversity", "Bacterial diversity", "archaeal diversity", "Soil particle size fractions", "0401 agriculture", " forestry", " and fisheries", "soil particle size fractions", "soil archaea", "16S rRNA gene amplicon sequencing"]}, "links": [{"href": "https://doi.org/2791089561"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2791089561", "name": "item", "description": "2791089561", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2791089561"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-02-23T00:00:00Z"}}, {"id": "3014863595", "type": "Feature", "geometry": null, "properties": {"updated": "2026-10-06T16:23:17Z", "type": "Journal Article", "created": "2020-02-15", "title": "Hide-and-seek with hoverflies: Merodon aureus \u2013 a species, a complex or a subgroup?", "description": "Abstract<p>In order to disentangle the currently confused interpretations and nomenclature of Merodon aureus and M. aeneus, we have reviewed all existing type material and species names known to us as assigned synonyms of these taxa. We resolve M. aeneus as being a junior synonym of M. aureus. We designate a lectotype for M. aureus and a neotype for M. aeneus. Additionally, we provide evidence that M. aureus, together with two newly discovered taxa (M. calidus sp. nov. and M. ortus sp. nov.), represent a complex of cryptic species named the M. aureus species complex. This complex, together with the M. unicolor species complex and the species M. pumilus, is part of the M. aureus subgroup. The M. unicolor species complex comprises two cryptic species: M. unicolor and M. albidus sp. nov. The new species are described by applying an integrative taxonomic approach using several data types (COI and 28S rRNA genes, geometric morphometry of the wings, ecological and distributional data). Based on the COI gene sequence analysis and distributional data, the pupa previously described as an immature stage of the species M. aureus is redefined as an immature stage of the new species M. calidus. Speciation within the M. aureus subgroup is discussed in the context of the phylogeographic history in the studied region.</p", "keywords": ["0106 biological sciences", "16. Peace & justice", "cryptic taxa", " distribution", " DNA sequences", " environmental niche modelling", " geometric morphometry", " identification key", " new species", " Palaearctic", "01 natural sciences"]}, "links": [{"href": "http://academic.oup.com/zoolinnean/article-pdf/190/3/974/34159192/zlaa016.pdf"}, {"href": "https://doi.org/3014863595"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Zoological%20Journal%20of%20the%20Linnean%20Society", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "3014863595", "name": "item", "description": "3014863595", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/3014863595"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-04T00:00:00Z"}}, {"id": "37499875", "type": "Feature", "geometry": null, "properties": {"updated": "2026-10-06T16:23:35Z", "type": "Journal Article", "created": "2023-07-25", "title": "Fast and reliable method to estimate global DNA methylation in plants and fungi with high-pressure liquid chromatography (HPLC)-ultraviolet detection and even more sensitive one with HPLC-mass spectrometry", "description": "DNA (Deoxyribonucleic acid) methylation is one of the epigenetic modifications of DNA, acting as a bridge between genotype and phenotype. Thus, disruption of DNA methylation pattern has tremendous consequences for organism development. Current methods to determine DNA methylation suffer from methodological drawbacks like high requirement of DNA and poor reproducibility of chromatograms. Here we provide a fast and reliable method using high-pressure liquid chromatography (HPLC)-ultraviolet (UV) detector and even more sensitive one with HPLC- mass spectrometry (MS) and we test this method with various plant and fungal DNA isolates. We optimized the preparation of the DNA degradation step to decrease background noise, we improved separation conditions to provide reliable and reproducible chromatograms and conditions to measure nucleotides in HPLC-MS. We showed that global DNA methylation level can be accurately and reproducibly measured with as little as 0.2\u00a0\u00b5M for HPLC-UV and 0.02\u00a0\u00b5M for HPLC-MS of methylated cytosine.", "keywords": ["Plant DNA", "DNA methylation", "ta1183", "ta1182", "Fungi", "610", "Reproducibility of Results", "DNA Methylation", "Mass Spectrometry", "Fungal DNA", "chromatography", "DNA", " Fungal", "ta116", "Chromatography", " High Pressure Liquid"]}, "links": [{"href": "https://doi.org/37499875"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "37499875", "name": "item", "description": "37499875", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/37499875"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-09-01T00:00:00Z"}}, {"id": "PMC5829042", "type": "Feature", "geometry": null, "properties": {"updated": "2026-10-06T16:25:00Z", "type": "Journal Article", "created": "2018-02-23", "title": "Bacterial Preferences for Specific Soil Particle Size Fractions Revealed by Community Analyses", "description": "Genetic fingerprinting demonstrated in previous studies that differently sized soil particle fractions (PSFs; clay, silt, and sand with particulate organic matter (POM)) harbor microbial communities that differ in structure, functional potentials and sensitivity to environmental conditions. To elucidate whether specific bacterial or archaeal taxa exhibit preference for specific PSFs, we examined the diversity of PCR-amplified 16S rRNA genes by high-throughput sequencing using total DNA extracted from three long-term fertilization variants (unfertilized, fertilized with minerals, and fertilized with animal manure) of an agricultural loamy sand soil and their PSFs. The PSFs were obtained by gentle ultrasonic dispersion, wet sieving, and centrifugation. The abundance of bacterial taxa assigned to operational taxonomic units (OTUs) differed less than 2.7% between unfractionated soil and soil based on combined PSFs. Across the three soil variants, no archaeal OTUs, but many bacterial OTUs, the latter representing 34-56% of all amplicon sequences, showed significant preferences for specific PSFs. The sand-sized fraction with POM was the preferred site for members of Bacteroidetes and Alphaproteobacteria, while Gemmatimonadales preferred coarse silt, Actinobacteria and Nitrosospira fine silt, and Planctomycetales clay. Firmicutes were depleted in the sand-sized fraction. In contrast, archaea, which represented 0.8% of all 16S rRNA gene sequences, showed only little preference for specific PSFs. We conclude that differently sized soil particles represent distinct microenvironments that support specific bacterial taxa and that these preferences could strongly contribute to the spatial heterogeneity and bacterial diversity found in soils.", "keywords": ["2. Zero hunger", "soil bacteria", "Soil bacteria", "soil DNA", "bacterial diversity", "04 agricultural and veterinary sciences", "Soil DNA", "15. Life on land", "Microbiology", "Soil archaea", "QR1-502", "Archaeal diversity", "Bacterial diversity", "archaeal diversity", "Soil particle size fractions", "0401 agriculture", " forestry", " and fisheries", "soil particle size fractions", "soil archaea", "16S rRNA gene amplicon sequencing"]}, "links": [{"href": "https://doi.org/PMC5829042"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC5829042", "name": "item", "description": "PMC5829042", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC5829042"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-02-23T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=+DNA&offset=50&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=+DNA&offset=50&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "prev", "title": "items (prev)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=+DNA&offset=0", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=+DNA&offset=56", "hreflang": "en-US"}], "numberMatched": 56, "numberReturned": 6, "distributedFeatures": [], "timeStamp": "2026-10-23T18:55:54.683574Z"}