{"type": "FeatureCollection", "features": [{"id": "10.1007/s11104-012-1547-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:50Z", "type": "Journal Article", "created": "2012-12-14", "title": "Snow Cover Manipulation Effects On Microbial Community Structure And Soil Chemistry In A Mountain Bog", "description": "Background and Aims  Alterations in snow cover driven by climate change may impact ecosystem functioning, including biogeochemistry and soil (microbial) processes. We elucidated the effects of snow cover manipulation (SCM) on above-and belowground processes in a temperate peatland.", "keywords": ["trends", "2. Zero hunger", "570", "biomass", "tundra soils", "variability", "[SDE.MCG]Environmental Sciences/Global Changes", "dynamics", "04 agricultural and veterinary sciences", "15. 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The PLFA extraction method can yield two additional lipid fractions\u2014neutral lipids and glycolipids\u2014which potentially hold additional, valuable information on soil microbial communities. Yet its quantitative sensitivity on complete neutral lipid (NLFA) and glycolipid fatty acid (GLFA) profiles has never been validated. In this study we tested (i) if the high-throughput PLFA method can be expanded to concurrently extract complete NLFA and GLFA profiles, as well as sterols, (ii) whether taxonomic specificities of signature fatty acids are retained across the three lipid fractions in pure culture strains, and (iii) whether NLFAs and GLFAs allow soil-specific fingerprinting to the same extent as PLFA analysis. By adjusting the polarity of chloroform with 2% ethanol for solid phase extraction, pure lipid standards were fully fractionated into neutral lipids, glycolipids, and phospholipids. Sterols eluted in the neutral lipid fraction, and a betaine lipid co-eluted with phospholipids. We found consistent taxonomic specificities of fatty acid markers across the three lipid fractions by analysing pure culture extracts representative of soil microbes. Fatty acid profiles from soil extracts, however, showed stronger differences between PLFAs, NLFAs, and GLFAs than between soil types. This indicates that PLFAs and NLFAs signify different community properties (biomass vs. carbon storage, putatively), and that GLFAs are sensitive markers for community traits which behave differently than PLFAs. Although we consistently found high abundances of characteristic sterols in fungal extracts, the PLFA extraction method only yielded miniscule amounts of ergosterol from soil extracts. 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An increase in the alkaline phosphatases and proteases linked to bacterial growth was displayed. This stimulation of microbial metabolism correlated with the degradation rates since TPHs degradation\u2019 efficiency after vermicompost addition reached 32.5% and 34.4% of the initial hydrocarbon levels for biostimulation and bioaugmentation, respectively. Although Polycyclic Aromatic", "keywords": ["Soil enzymes", "01 natural sciences", "Total petroleum hydrocarbons polluted soils", "Soil", "Bioaugmentation", "Alkanes", "Soil Pollutants", "Micronutrients", "Polycyclic Aromatic Hydrocarbons", "Materials", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences", "2. Zero hunger", "Minerals", "Materiales", "Biostimulation", " Bioaugmentation", " Phospholipid fatty acids", " Total petroleum hydrocarbons polluted soils", " Soil enzymes", "Fatty Acids", "Phosphorus", "Phenanthrenes", "Hydrocarbons", "Phosphoric Monoester Hydrolases", "6. 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As landscape-scale fuel reduction treatments are being implemented throughout the fire-prone western United States of America, it is important to evaluate operationally how these wildfire mitigation treatments alter belowground processes. We quantified these important belowground components before and after management-applied fuel treatments of thinning alone, thinning combined with prescribed fire, and prescribed fire in ponderosa pine (Pinus ponderosa) stands at the Southwest Plateau, Fire and Fire Surrogate site, Arizona. Fuel treatments did not alter pH, total carbon and nitrogen (N) concentrations, or base cations of the forest floor (O horizon) or mineral soil (0\uffe2\uff80\uff935 cm) during this 2-year study. In situ rates of net N mineralization and nitrification in the surface mineral soil (0\uffe2\uff80\uff9315 cm) increased 6 months after thinning with prescribed fire treatments; thinning only resulted in net N immobilization. The rates returned to pre-treatment levels after one year. Based on phospholipid fatty acid composition, microbial communities in treated areas were similar to untreated areas (control) in the surface organic horizon and mineral soil (0\uffe2\uff80\uff935 cm) after treatments. Soil potential enzyme activities were not significantly altered by any of the three fuel treatments. Our results suggest that a variety of one-time alternative fuel treatments can reduce fire hazard without degrading soil fertility.</p>", "keywords": ["2. Zero hunger", "Ecology", "Life on Land", "fuel treatments", "13. Climate action", "Forestry Sciences", "fuel treatments; nitrification; nitrogen mineralization; phospholipid fatty acids; soil enzymes", "Plant Biology", "phospholipid fatty acids", "15. Life on land", "soil enzymes", "nitrification", "nitrogen mineralization"], "contacts": [{"organization": "Overby, Steven T, Hart, Stephen C,", "roles": ["creator"]}]}, "links": [{"href": "http://www.mdpi.com/1999-4907/7/2/45/pdf"}, {"href": "https://escholarship.org/content/qt54s4m5pt/qt54s4m5pt.pdf"}, {"href": "https://doi.org/10.3390/f7020045"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Forests", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/f7020045", "name": "item", "description": "10.3390/f7020045", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/f7020045"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-02-18T00:00:00Z"}}, {"id": "10.5281/zenodo.6934168", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:23:31Z", "type": "Dataset", "title": "In Situ Diversity of Metabolism and Carbon Use Efficiency among Soil Bacteria", "description": "Dataset for the MS", "keywords": ["Soil microbiology", " metabolic flux analysis", " phospholipid fatty acids"], "contacts": [{"organization": "Wu, Weichao, Dijkstra, Paul, Hungate, Bruce A., Lingling Shi, Dippold, Michaela,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.6934168"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.6934168", "name": "item", "description": "10.5281/zenodo.6934168", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.6934168"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-07-29T00:00:00Z"}}, {"id": "10.5281/zenodo.6934169", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:23:31Z", "type": "Dataset", "title": "In Situ Diversity of Metabolism and Carbon Use Efficiency among Soil Bacteria", "description": "Dataset for the MS", "keywords": ["Soil microbiology", " metabolic flux analysis", " phospholipid fatty acids"], "contacts": [{"organization": "Wu, Weichao, Dijkstra, Paul, Hungate, Bruce A., Lingling Shi, Dippold, Michaela,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.6934169"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.6934169", "name": "item", "description": "10.5281/zenodo.6934169", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.6934169"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-07-29T00:00:00Z"}}, {"id": "10259/7423", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:24:34Z", "type": "Journal Article", "created": "2022-05-28", "title": "Evaluation of Biostimulation, Bioaugmentation, and Organic Amendments Application on the Bioremediation of Recalcitrant Hydrocarbons of Soil", "description": "In the present work, the operational conditions for improving the degradation rates of Total Petroleum Hydrocarbons (TPHs) in contaminated soil from a machinery park were optimized at a microcosms scale along a 90- days incubation period. 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An increase in the alkaline phosphatases and proteases linked to bacterial growth was displayed. This stimulation of microbial metabolism correlated with the degradation rates since TPHs degradation\u2019 efficiency after vermicompost addition reached 32.5% and 34.4% of the initial hydrocarbon levels for biostimulation and bioaugmentation, respectively. Although Polycyclic Aromatic", "keywords": ["Soil enzymes", "01 natural sciences", "Total petroleum hydrocarbons polluted soils", "Soil", "Bioaugmentation", "Alkanes", "Soil Pollutants", "Micronutrients", "Polycyclic Aromatic Hydrocarbons", "Materials", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences", "2. Zero hunger", "Minerals", "Materiales", "Fatty Acids", "Phosphorus", "Phenanthrenes", "Hydrocarbons", "Phosphoric Monoester Hydrolases", "6. Clean water", "Biostimulation", "Biodegradation", " Environmental", "Petroleum", "13. 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The PLFA extraction method can yield two additional lipid fractions\u2014neutral lipids and glycolipids\u2014which potentially hold additional, valuable information on soil microbial communities. Yet its quantitative sensitivity on complete neutral lipid (NLFA) and glycolipid fatty acid (GLFA) profiles has never been validated. In this study we tested (i) if the high-throughput PLFA method can be expanded to concurrently extract complete NLFA and GLFA profiles, as well as sterols, (ii) whether taxonomic specificities of signature fatty acids are retained across the three lipid fractions in pure culture strains, and (iii) whether NLFAs and GLFAs allow soil-specific fingerprinting to the same extent as PLFA analysis. By adjusting the polarity of chloroform with 2% ethanol for solid phase extraction, pure lipid standards were fully fractionated into neutral lipids, glycolipids, and phospholipids. Sterols eluted in the neutral lipid fraction, and a betaine lipid co-eluted with phospholipids. We found consistent taxonomic specificities of fatty acid markers across the three lipid fractions by analysing pure culture extracts representative of soil microbes. Fatty acid profiles from soil extracts, however, showed stronger differences between PLFAs, NLFAs, and GLFAs than between soil types. This indicates that PLFAs and NLFAs signify different community properties (biomass vs. carbon storage, putatively), and that GLFAs are sensitive markers for community traits which behave differently than PLFAs. Although we consistently found high abundances of characteristic sterols in fungal extracts, the PLFA extraction method only yielded miniscule amounts of ergosterol from soil extracts. 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