{"type": "FeatureCollection", "features": [{"id": "10.1016/j.jaap.2018.11.026", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:16:09Z", "type": "Journal Article", "created": "2018-11-27", "title": "Influence of step duration in fractionated Py-GC/MS of lignocellulosic biomass", "description": "<p>Fractionated pyrolysis coupled to gas chromatography and mass spectrometry (Py-GC/MS) appears as an interesting analytical tool for elucidating lignocellulosic biomass structure, as it allows the progressive release of chemical fragments representative of biomass macromolecular composition. In this paper the effect of fractionated pyrolysis time (from 5 s to 300 s) on the degradation of lignin and carbohydrates from beech wood was studied at temperatures between 250 \u00b0C and 500 \u00b0C. Fractionated Py-GC/MS showed that the release temperature of the volatile degradation products varied between the volatile species detected. In addition, the step duration time changed the thermal degradation behavior of lignocellulosic components. Shortening the constant step duration time from 300 s to 5 s shifted the maximum weight loss to the higher temperatures. The result was opposite at long step duration times. Time optimization at each pyrolysis temperature (250 \u00b0C, 40 s; 300 \u00b0C, 30 s; 350 \u00b0C, 25 s; 370 \u00b0C, 20 s; 400 \u00b0C, 15 s; 450 \u00b0C, 10 s; 500 \u00b0C, 5 s) enhanced the yield of both lignin and carbohydrate volatile pyrolysis degradation products. In addition, two multiple temperature maxima were shown for some lignin and carbohydrate derivatives. This behavior may be due to the two different pathways of formation and macromolecular origins of compounds in beech wood. At optimized conditions lignin derivatives having a 3-carbon side chain substituent had a maximum at lower temperature than that of lignin derivatives with a 1-carbon side chain substituent. That phenomenon follows the order of primary and secondary pyrolysis reactions. Similar behaviors were observed among the degradation products of hemicelluloses and cellulose. Degradation products of hemicelluloses were mainly released at lower temperatures than those of cellulose derivatives, which illustrates the lower thermal stability of hemicelluloses compared to cellulose.</p>", "keywords": ["Beech", "ta114", "[CHIM.GENI] Chemical Sciences/Chemical engineering", "[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering", "Fractionated pyrolysis", "Carbohydrates", "0211 other engineering and technologies", "02 engineering and technology", "540", "Py-GC/MS", "Lignin", "7. Clean energy", "13. Climate action", "Thermal degradation", "0202 electrical engineering", " electronic engineering", " information engineering", "G\u00e9nie chimique", "SDG 7 - Affordable and Clean Energy", "G\u00e9nie des proc\u00e9d\u00e9s", "ta116", "ta215"]}, "links": [{"href": "https://oatao.univ-toulouse.fr/27243/1/GonzalezMartinez_27243.pdf"}, {"href": "https://doi.org/10.1016/j.jaap.2018.11.026"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Analytical%20and%20Applied%20Pyrolysis", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jaap.2018.11.026", "name": "item", "description": "10.1016/j.jaap.2018.11.026", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jaap.2018.11.026"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-01-01T00:00:00Z"}}, {"id": "10.1007/s10745-009-9241-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:14:59Z", "type": "Journal Article", "created": "2009-05-27", "title": "Swidden Transformations And Rural Livelihoods In Southeast Asia", "description": "This paper explores the major interactions between the transformation of swidden farming and the pursuit of rural livelihoods in the uplands of Southeast Asia. The paper draws on selected literature, workshop reflections, and six case studies to describe the causal processes and livelihood consequences of swidden change. Household-level livelihood responses have included both the intensification and \u2018dis-intensification\u2019 of swidden land-use, the insertion of cash crops, the redeployment of household labour, and the taking on of broader (often non-rural) livelihood aspirations and strategies. At the community level there have been emerging institutional arrangements for management of land and forests, and varying degrees of participation in or resistance to government schemes and programs. Swidden change has led to the loss and also the reassertion, realignment, and redefinition of cultures and identities, with important implications for access to resources. The impacts of these changes have been varied. Cash crops have often improved livelihoods but complete specialisation for the market increases vulnerability. Thus swidden can still provide an important safety net in the face of market fluctuations. Improved access to markets and social provision of education and health care have mostly improved the welfare of previously isolated groups. However, growing differences within and between communities in the course of swidden transformations can leave some groups marginalized and worse off. These processes of differentiation can be accentuated by heavy-handed state interventions based on swidden stereotypes. Nevertheless, communities have not passively accepted these pressures and have mobilized to protect their livelihood assets and strategies. Thus swidden farmers are not resisting appropriate and supportive forms of development. They are adopting new practices and engaging with markets, but in many situations swidden is still important to their livelihood strategies, providing resilience in the face of turbulent change. Active involvement of local people is essential in planning, implementing, monitoring and evaluating development and conservation programs in swidden lands. Positive market incentives and supportive government policies are better than standardised, top-down directives.", "keywords": ["0211 other engineering and technologies", "02 engineering and technology", "910", "livelihoods", "01 natural sciences", "12. Responsible consumption", "Livelihood strategies", "C1", "agricultural development", "11. Sustainability", "0105 earth and related environmental sciences", "2. Zero hunger", "Agrarian change", "160804 Rural Sociology", "Uplands", "919999 Economic Framework not elsewhere classified", "upland areas", "1. No poverty", "1601 Anthropology", "160499 Human Geography not elsewhere classified", "Food security", "food security", "Resource tenure", "15. Life on land", "Agency", "governance", "970116 Expanding Knowledge through Studies of Human Society"]}, "links": [{"href": "https://doi.org/10.1007/s10745-009-9241-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Human%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10745-009-9241-6", "name": "item", "description": "10.1007/s10745-009-9241-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10745-009-9241-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-05-28T00:00:00Z"}}, {"id": "10.1007/s10811-017-1175-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:15:00Z", "type": "Journal Article", "created": "2017-06-06", "title": "Comparison of Scenedesmus acuminatus and Chlorella vulgaris cultivation in liquid digestates from anaerobic digestion of pulp and paper industry and municipal wastewater treatment sludge", "description": "Two microalgae, Chlorella vulgaris and Scenedesmus acuminatus, were batch cultivated separately in two types of diluted liquid digestates. The first digestate (ADPP) was obtained from a mesophilic laboratory digester treating biosludge from a pulp and paper industry wastewater treatment plant. The second digestate (ADMW) was collected from a full-scale mesophilic anaerobic digester treating-mixed municipal wastewater treatment sludge. The highest biomass production (as volatile suspended solids, VSS), 8.2\u20139.4\u00a0g\u00a0L\u22121, was obtained with S. acuminatus in ADPP. C. vulgaris in ADMW had the lowest biomass production, reaching 2.0\u00a0g\u00a0L\u22121. Both microalgae removed ammonium efficiently from ADPP (99.9% removal) while the final ammonium removal efficiencies from ADMW with S. acuminatus and C. vulgaris were only 44.0 and 23.8%, respectively. The phosphate removal efficiencies from both ADPP and ADMW were higher than 96.9% with both microalgae. The highest carbohydrate content (60.5%) was obtained with S. acuminatus cultivated in ADPP. Scenedesmus acuminatus in ADPP showed one of the highest biomass production yields that have been reported for microalgae in real wastewater-derived nutrient sources. Consequently, this combination is promising for developing biorefinery and biofuel applications in the pulp and paper industry.", "keywords": ["330", "116 Chemical sciences", "11. Sustainability", "116", "01 natural sciences", "7. Clean energy", "6. Clean water", "0105 earth and related environmental sciences", "12. Responsible consumption"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s10811-017-1175-6.pdf"}, {"href": "https://doi.org/10.1007/s10811-017-1175-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Applied%20Phycology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10811-017-1175-6", "name": "item", "description": "10.1007/s10811-017-1175-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10811-017-1175-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-06-06T00:00:00Z"}}, {"id": "10.1007/s11130-020-00799-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:15:08Z", "type": "Journal Article", "created": "2020-02-03", "title": "Profile and Content of Residual Alkaloids in Ten Ecotypes of Lupinus mutabilis Sweet after Aqueous Debittering Process", "description": "Abstract<p>The evaluation of the level of alkaloids in edible Lupinus species is crucial from a food safety point of view. Debittering of lupin seeds has a long history; however, the control of the level of alkaloids after processing the seeds is typically only evaluated by changes in the bitter taste. The aim of this study was to evaluate the profile and residual levels of quinolizidine alkaloids (QA) in (Lupinus mutabilis Sweet) after aqueous debittering process. Samples from 10 ecotypes from different areas of Peru were analyzed before and after the process. Based on results obtained by gas chromatography and mass spectrometry, from eight alkaloids identified before the debittering process, only small amounts of lupanine (avg. 0.0012\uffc2\uffa0g/100\uffc2\uffa0g DM) and sparteine (avg. 0.0014\uffc2\uffa0g/100\uffc2\uffa0g DM) remained in the seeds after the debittering process, and no other alkaloids were identified. The aqueous debittering process reduced the content of alkaloids to levels far below the maximal level allowed by international regulations (\uffe2\uff89\uffa4 0.2\uffc2\uffa0g/kg DM).</p>", "keywords": ["0301 basic medicine", "Lupin Seeds", "Sparteine", "Organic chemistry", "Propiedades fisicoqu\u00edmicas", "Plant Science", "Gas Chromatography-Mass Spectrometry", "Evolution and Nutritional Properties of Lupin Seeds", "Agricultural and Biological Sciences", "Food science", "Per\u00fa", "03 medical and health sciences", "Deshidrataci\u00f3n acuosa", "Alkaloids", "Secado", "Tarwi", "https://purl.org/pe-repo/ocde/ford#2.11.01", "Granos", "Composici\u00f3n qu\u00edmica", "ta116", "Biology", "Ecotipos", "Ecology", " Evolution", " Behavior and Systematics", "Ecotype", "2. Zero hunger", "Original Paper", "0303 health sciences", "Rendimiento", "Procesamiento", "Evaluaci\u00f3n", "ta1183", "An\u00e1lisis organol\u00e9ptico", "ta1182", "Botany", "Life Sciences", "Diversity and Applications of Cyperus Species", "Lupinus", "Chocho", "Chemistry", "Contenido proteico", "Evolution and Ecology of Endophyte-Grass Symbiosis", "Taste", "Seeds", "Lupinus mutabilis"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s11130-020-00799-y.pdf"}, {"href": "https://doi.org/10.1007/s11130-020-00799-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Foods%20for%20Human%20Nutrition", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11130-020-00799-y", "name": "item", "description": "10.1007/s11130-020-00799-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11130-020-00799-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-02-03T00:00:00Z"}}, {"id": "10.1007/s12649-017-9910-4", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:15:14Z", "type": "Journal Article", "created": "2017-03-25", "title": "Possibilities of Using Liquids from Slow Pyrolysis and Hydrothermal Carbonization in Acidification of Animal Slurry", "description": "Pyrolysis and hydrothermal carbonization (HTC) are gaining increasing interest in the context of biomass valorization. However, these processes yield a liquid fraction with an acidic pH value challenging to productize. In this paper, the possibility of replacing concentrated acids in acidification of animal slurry with organic acids derived from thermochemical conversion of various biomasses was investigated. The acid composition of four pyrolysis and one HTC liquid fraction were characterized using capillary electrophoresis and their total acidity determined titrimetrically. The amount of each liquid needed to reduce the pH of pig and cattle slurries to 6.0 and 5.5 were recorded. The total acidity of pyrolysis liquids varied highly (850-2560 meq l<sup>-1</sup>) depending on the biomass. For HTC liquid the total acidity was low (220 meq l<sup>-1</sup>). The most concentrated liquid, which showed greatest potential for practical use, was derived from pyrolysis of willow wood. Its required application rate for decreasing the pH of the slurries from &gt;7.5 to 6.0 was 20-50 l t<sup>-1</sup>. This study suggests that there is a possibility of using liquids from pyrolysis process for acidification of animal slurries. Producing more concentrated liquids, further concentration of these acidiferous streams and the effects of the liquid addition on the properties of slurry are worth further studying.", "keywords": ["ta412", "600", "02 engineering and technology", "kuivatislaus", "pyrolysis", "01 natural sciences", "hydrothermal carbonization", "acidification", "13. Climate action", "happamoituminen", "0202 electrical engineering", " electronic engineering", " information engineering", "SDG 7 - Affordable and Clean Energy", "ta116", "animal slurry", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s12649-017-9910-4.pdf"}, {"href": "https://doi.org/10.1007/s12649-017-9910-4"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Waste%20and%20Biomass%20Valorization", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s12649-017-9910-4", "name": "item", "description": "10.1007/s12649-017-9910-4", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s12649-017-9910-4"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-03-25T00:00:00Z"}}, {"id": "10.1016/j.agee.2017.05.005", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:15:23Z", "type": "Journal Article", "created": "2017-05-16", "title": "Evaluating The Effect Of Shade Trees On Provision Of Ecosystem Services In Intensively Managed Coffee Plantations", "description": "Abstract   Intensively managed cropping systems with emphasis on productivity of the main crop can benefit from additional ecosystem services brought by integration of trees in the system \u2212 but potential drawbacks must also be accounted for. In an on-farm study, we used a variety of plant, soil and water- related variables to assess the effect of Erythrina spp. and Musa spp. on the provision of ecosystem services in productive, high-quality Coffea arabica plantations in Costa Rica. We found 1) no significant effect of shade trees on coffee production overall; 2) evidence that shade trees do affect flowering and subsequent cherry development, with effects strongly dependent on climate and annual variations in coffee plant physiology; 3) Erythrina shade trees significantly increased soil litter and relative infiltration rate of water in the soil, both linked to soil conservation and decrease in erosion; 4) even in highly fertilized environments, Erythrina trees do fix N which was taken up by adjacent coffee plants. The lack of significant negative effect of shade trees on overall coffee yield and the observation of the provision of other useful services was not unexpected, because of 1) the low density of shade trees in the study site (100\u2013350 trees/ha pruned twice a year on average) and 2) the sensitivity of coffee yields to other interacting effects such as climate, pests and diseases and physiological variations in the plant. Pending further long-term research into the factors affecting coffee yield, we find shade trees provide sufficient ecosystem services to justify their integration in even intensively managed plantations.", "keywords": ["[SDV.SA]Life Sciences [q-bio]/Agricultural sciences", "0106 biological sciences", "F08 - Syst\u00e8mes et modes de culture", "http://aims.fao.org/aos/agrovoc/c_1920", "http://aims.fao.org/aos/agrovoc/c_2658", "adaptation aux changements climatiques", "910", "\u00e9cosyst\u00e8me agricole", "01 natural sciences", "630", "agroforestry", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "http://aims.fao.org/aos/agrovoc/c_1721", "Erythrina", "http://aims.fao.org/aos/agrovoc/c_35657", "agroforesterie", "2. Zero hunger", "[SDV.SA] Life Sciences [q-bio]/Agricultural sciences", "transport des substances nutritives", "liti\u00e8re foresti\u00e8re", "http://aims.fao.org/aos/agrovoc/c_2328", "caf\u00e9", "Coffea arabica", "04 agricultural and veterinary sciences", "fertilit\u00e9 du sol", "N fixation", "http://aims.fao.org/aos/agrovoc/c_5272", "http://aims.fao.org/aos/agrovoc/c_5196", "mati\u00e8re organique du sol", "planting", "P33 - Chimie et physique du sol", "Fixation de l'azote", "r\u00e9sistance aux maladies", "http://aims.fao.org/aos/agrovoc/c_7170", "coffee", "plantation", "cycle hydrologique", "yield components", "arbre d'ombrage", "http://aims.fao.org/aos/agrovoc/c_1374567058134", "http://aims.fao.org/aos/agrovoc/c_2392", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "Theobroma cacao", "http://aims.fao.org/aos/agrovoc/c_2992", "http://aims.fao.org/aos/agrovoc/c_3047", "tradeoff", "floraison", "P35 - Fertilit\u00e9 du sol", "http://aims.fao.org/aos/agrovoc/c_25548", "http://aims.fao.org/aos/agrovoc/c_1348040570280", "15. Life on land", "K10 - Production foresti\u00e8re", "services \u00e9cosyst\u00e9miques", "r\u00e9sistance \u00e0 la s\u00e9cheresse", "http://aims.fao.org/aos/agrovoc/c_11670", "http://aims.fao.org/aos/agrovoc/c_7713", "0401 agriculture", " forestry", " and fisheries", "ecosystem services"]}, "links": [{"href": "https://doi.org/10.1016/j.agee.2017.05.005"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2017.05.005", "name": "item", "description": "10.1016/j.agee.2017.05.005", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2017.05.005"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-07-01T00:00:00Z"}}, {"id": "10.1016/j.biortech.2017.08.218", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-22T16:15:35Z", "type": "Journal Article", "created": "2017-09-04", "title": "Cultivation of Scenedesmus acuminatus in different liquid digestates from anaerobic digestion of pulp and paper industry biosludge", "description": "Different undiluted liquid digestates from mesophilic and thermophilic anaerobic digesters of pulp and paper industry biosludge with and without thermal pretreatment were characterized and utilized for cultivating Scenedesmus acuminatus. Higher S. acuminatus biomass yields were obtained in thermophilic digestates (without and with pretreatment prior to anaerobic digestion (AD): 10.2\u00b12.2 and 10.8\u00b11.2gL-1, respectively) than in pretreated mesophilic digestates (7.8\u00b10.3gL-1), likely due to differences in concentration of sulfate, iron, and/or other minor nutrients. S. acuminatus removed over 97.4% of ammonium and 99.9% of phosphate and sulfate from the digestates. Color (74-80%) and soluble COD (29-39%) of the digestates were partially removed. Different AD processes resulted in different methane yields (18-126L CH4 kg-1VS), digestate compositions, and microalgal yields. These findings emphasize the importance of optimizing each processing step in wood-based biorefineries and provide information for pulp and paper industry development for enhancing value generation.", "keywords": ["13. Climate action", "116 Chemical sciences", "Microalgae", "116", "Anaerobiosis", "Biomass", "Methane", "7. Clean energy", "01 natural sciences", "Scenedesmus", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.biortech.2017.08.218"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biortech.2017.08.218", "name": "item", "description": "10.1016/j.biortech.2017.08.218", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biortech.2017.08.218"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-12-01T00:00:00Z"}}, {"id": "10.1016/j.cej.2019.04.090", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-22T16:15:37Z", "type": "Journal Article", "created": "2019-04-15", "title": "Effects of anode materials on electricity production from xylose and treatability of TMP wastewater in an up-flow microbial fuel cell", "description": "Abstract   The aim of this study was to determine an optimal anode material for electricity production and COD removal from xylose containing synthetic wastewater in an up-flow microbial fuel cell (MFC), and assess its suitability for treatment of thermomechanical pulping (TMP) wastewater with an enrichment culture at 37\u202f\u00b0C. The anode materials tested included carbon-based electrodes (graphite plate, carbon cloth and zeolite coated carbon cloth), metal-based electrodes (tin coated copper) and a metal-carbon assembly (granular activated carbon in stainless steel cage). During continuous operation with xylose, COD removal was 77\u201386% of which 25\u201328% was recovered as electricity. The highest power density of 333\u202f(\u00b115)\u202fmW/m2 was obtained with the carbon cloth electrode. However, based on an overall analysis including electrode performance, surface area and scalability, the granular activated carbon in stainless steel cage (GAC in SS cage) was chosen to be used as electrode for bioelectrochemical treatment of TMP wastewater. The TMP fed MFC was operated in continuous mode with 1.8\u202fdays hydraulic retention time, resulting in 47\u202f(\u00b113%) COD removal of which 1.5% was recovered as electricity with the average power production of 10\u201315\u202fmW/m2. During operation with TMP wastewater, membrane fouling increased the polarization resistance causing a 50% decrease in power production within 30\u202fdays. This study shows that MFC pretreatment removes half of the TMP wastewater COD load, reducing the energy required for aerobic treatment.", "keywords": ["116 Chemical sciences", "01 natural sciences", "7. Clean energy", "6. Clean water", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.cej.2019.04.090"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemical%20Engineering%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.cej.2019.04.090", "name": "item", "description": "10.1016/j.cej.2019.04.090", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.cej.2019.04.090"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-09-01T00:00:00Z"}}, {"id": "10.1016/j.jbiotec.2023.07.008", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:16:10Z", "type": "Journal Article", "created": "2023-07-25", "title": "Fast and reliable method to estimate global DNA methylation in plants and fungi with high-pressure liquid chromatography (HPLC)-ultraviolet detection and even more sensitive one with HPLC-mass spectrometry", "description": "DNA (Deoxyribonucleic acid) methylation is one of the epigenetic modifications of DNA, acting as a bridge between genotype and phenotype. Thus, disruption of DNA methylation pattern has tremendous consequences for organism development. Current methods to determine DNA methylation suffer from methodological drawbacks like high requirement of DNA and poor reproducibility of chromatograms. Here we provide a fast and reliable method using high-pressure liquid chromatography (HPLC)-ultraviolet (UV) detector and even more sensitive one with HPLC- mass spectrometry (MS) and we test this method with various plant and fungal DNA isolates. We optimized the preparation of the DNA degradation step to decrease background noise, we improved separation conditions to provide reliable and reproducible chromatograms and conditions to measure nucleotides in HPLC-MS. We showed that global DNA methylation level can be accurately and reproducibly measured with as little as 0.2\u00a0\u00b5M for HPLC-UV and 0.02\u00a0\u00b5M for HPLC-MS of methylated cytosine.", "keywords": ["Chromatography", "Plant DNA", "DNA methylation", "ta1183", "ta1182", "Fungi", "610", "Reproducibility of Results", "DNA Methylation", "Mass Spectrometry", "Fungal DNA", "chromatography", "DNA", " Fungal", "ta116", "Chromatography", " High Pressure Liquid"]}, "links": [{"href": "https://doi.org/10.1016/j.jbiotec.2023.07.008"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jbiotec.2023.07.008", "name": "item", "description": "10.1016/j.jbiotec.2023.07.008", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jbiotec.2023.07.008"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-09-01T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2018.12.062", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:16:13Z", "type": "Journal Article", "created": "2018-12-18", "title": "H2S removal and microbial community composition in an anoxic biotrickling filter under autotrophic and mixotrophic conditions", "description": "Removal of H2S from gas streams using NO3--containing synthetic wastewater was investigated in an anoxic biotrickling filter (BTF) at feed N/S ratios of 1.2-1.7\u2009mol mol-1 with an empty bed residence time of 3.5\u2009min and a hydraulic retention time of 115\u2009min. During 108 days of operation under autotrophic conditions, the BTF showed a maximum elimination capacity (EC) of 19.2\u2009g S m-3\u2009h-1 and H2S removal efficiency (RE) >99%. When the BTF was operated under mixotrophic conditions by adding organic carbon (10.2\u2009g acetate m-3\u2009h-1) to the synthetic wastewater, the H2S EC decreased from 16.4 to 13.1\u2009g S m-3\u2009h-1, while the NO3- EC increased from 9.9 to 11.1\u2009g NO3--N m-3\u2009h-1, respectively. Thiobacillus sp. (98-100% similarity) was the only sulfur-oxidizing nitrate-reducing bacterium detected in the BTF biofilm, while the increased abundance of heterotrophic denitrifiers, i.e. Brevundimonas sp. and Rhodocyclales, increased the N/S ratio during BTF operation. Residence time distribution tests showed that biomass accumulation during BTF operation reduced gas and liquid retention times by 17.1% and 83.5%, respectively.", "keywords": ["570", "Air Pollutants", "Nitrates", "550", "Bacteria", "Microbiota", "116 Chemical sciences", "116", "Waste Disposal", " Fluid", "01 natural sciences", "6. Clean water", "Bioreactors", "Denitrification", "Hydrogen Sulfide", "Filtration", "Water Pollutants", " Chemical", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.jhazmat.2018.12.062"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2018.12.062", "name": "item", "description": "10.1016/j.jhazmat.2018.12.062", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2018.12.062"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-01T00:00:00Z"}}, {"id": "10.1016/j.foreco.2018.11.033", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:15:59Z", "type": "Journal Article", "created": "2018-11-29", "title": "Impacts of forests and forestation on hydrological services in the Andes: A systematic review", "description": "Abstract   Several Andean countries have planned to restore forest cover in degraded land to enhance the provision of multiple ecosystem services in response to international commitments such as the Bonn Challenge. Hydrological services, e.g. water supply, hydrological regulation and erosion mitigation, are particularly important to sustain the life of more than fifty million Andean people. While rapid and important forest cover changes have occurred during recent decades, critical information on the impact of forestation on hydrological services has not yet been synthesized in the context of Andean ecosystems. We define forestation as the establishment of forest by plantation or natural regeneration on areas that either had forest in the past or not. To help improve decision-making on forestation in the Andes, we reviewed the available literature concerning the impacts of forestation on water supply, hydrological regulation and mitigation of erosion and landslides. We also examined available data on the most relevant hydrological processes such as infiltration, evapotranspiration and runoff in forest stands. Hydrological services from native forests were also included as a reference state for comparing processes and services provided by forestation. Following systematic review protocols, we synthesized 155 studies using different methods, including meta-analyses and meta-regressions. Results show that forestation has had clear impacts on degraded soils, through reducing water erosion of soils and risk of moderate floods, increasing soil infiltration rate by 8 and topsoil organic matter (SOM). We found that 20\u202fyears of tree plantation was sufficient to recover infiltration rate and sediment yield close to the levels of native forests whereas SOM, soil water storage and surface runoff of native forests could not be recovered by forestation in the time scales examined. The benefits in terms of hydrological regulation are at the expense of a reduction in total water supply since forest cover was associated with higher water use in most Andean regions. Forestation with native species was underrepresented in the reviewed studies. The impact of forestation on landslides has also been largely overlooked in the Andes. At high elevations, exotic tree plantations on Andean grasslands (e.g. paramo and puna) had the most detrimental consequences since these grasslands showed an excellent capacity for hydrological regulation and erosion mitigation but also a water yield up to 40% higher than tree plantations. People engaged in forest restoration initiative should be aware that hydrological services may take some time for society and the environment to show clear benefits after forestation.", "keywords": ["P33 - Chimie et physique du sol", "Pine plantations", "forest rehabilitation", "propri\u00e9t\u00e9 physicochimique du sol", "550", "F40 - \u00c9cologie v\u00e9g\u00e9tale", "Monitoring", "Ecosystem service", "[SDE.MCG]Environmental Sciences/Global Changes", "ecological restoration", "05 Environmental Sciences", "systematic reviews", "0207 environmental engineering", "forest cover", "hydrology", "02 engineering and technology", "hydrologie", "01 natural sciences", "630", "cycle hydrologique", "http://aims.fao.org/aos/agrovoc/c_3062", "for\u00eat", "K01 - Foresterie - Consid\u00e9rations g\u00e9n\u00e9rales", "11. Sustainability", "http://aims.fao.org/aos/agrovoc/c_13802", "reconstitution foresti\u00e8re", "P10 - Ressources en eau et leur gestion", "Land-use", "Nature and Landscape Conservation", "0105 earth and related environmental sciences", "forests", "P36 - \u00c9rosion", " conservation et r\u00e9cup\u00e9ration des sols", "2. Zero hunger", "Policy and Law", "http://aims.fao.org/aos/agrovoc/c_7182", "Forestry", "http://aims.fao.org/aos/agrovoc/c_401", "06 Biological Sciences", "15. Life on land", "6. Clean water", "Management", "http://aims.fao.org/aos/agrovoc/c_11670", "[SDE.MCG] Environmental Sciences/Global Changes", "13. Climate action", "degraded land", "07 Agricultural And Veterinary Sciences", "http://aims.fao.org/aos/agrovoc/c_3731"]}, "links": [{"href": "https://doi.org/10.1016/j.foreco.2018.11.033"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Forest%20Ecology%20and%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.foreco.2018.11.033", "name": "item", "description": "10.1016/j.foreco.2018.11.033", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.foreco.2018.11.033"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-01T00:00:00Z"}}, {"id": "10.1016/j.ijhydene.2017.12.002", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-22T16:16:07Z", "type": "Journal Article", "created": "2018-02-03", "title": "Quantitative real-time PCR monitoring dynamics of Thermotoga neapolitana in synthetic co-culture for biohydrogen production", "description": "Abstract   This study demonstrates the potential for biohydrogen production in a co-culture of two ecologically distant species, Thermatoga neapolitana and Caldicellulosiruptor saccharolyticus, and the development of a quantitative real-time PCR (qPCR) method for quantifying the hyperthermophilic bacterium of the genus Thermotoga. Substrate utilization and H2 production performance was compared to those of their individual cultures. The highest H2 yields obtained were 2.7\u00a0\u00b1\u00a00.05, 2.5\u00a0\u00b1\u00a00.07 and 2.8\u00a0\u00b1\u00a00.09\u00a0mol H2/mol glucose for C. saccharolyticus, T. neapolitana, and their co-culture respectively. Statistical analysis comparing the H2 production rate of the co-culture to either C. saccahrolyticus or T. neapolitana pure cultures indicated a significant difference in the H2 production rate (p", "keywords": ["220 Industrial biotechnology", "0301 basic medicine", "2. Zero hunger", "570", "03 medical and health sciences", "220", "116 Chemical sciences", "116", "01 natural sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.ijhydene.2017.12.002"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Hydrogen%20Energy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijhydene.2017.12.002", "name": "item", "description": "10.1016/j.ijhydene.2017.12.002", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijhydene.2017.12.002"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-02-01T00:00:00Z"}}, {"id": "10.1016/j.ijhydene.2018.01.158", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-22T16:16:07Z", "type": "Journal Article", "created": "2018-03-02", "title": "Thermophilic versus mesophilic dark fermentation in xylose-fed fluidised bed reactors: Biohydrogen production and active microbial community", "description": "Abstract   Dark fermentative biohydrogen production in a thermophilic, xylose-fed (50\u00a0mM) fluidised bed reactor (FBR) was evaluated in the temperature range 55\u201370\u00a0\u00b0C with 5-degree increments and compared with a mesophilic FBR operated constantly at 37\u00a0\u00b0C. A significantly higher (p\u00a0=\u00a00.05) H2 yield was obtained in the thermophilic FBR, which stabilised at about 1.2\u00a0mol H2\u00a0mol\u22121 xylose (36% of the theoretical maximum) at 55 and 70\u00a0\u00b0C, and at 0.8\u00a0mol H2\u00a0mol\u22121 xylose at 60 and 65\u00a0\u00b0C, compared to the mesophilic FBR (0.5\u00a0mol H2\u00a0mol\u22121 xylose). High-throughput sequencing of the reverse-transcribed 16S rRNA, done for the first time on biohydrogen producing reactors, indicated that Thermoanaerobacterium was the prevalent active microorganism in the thermophilic FBR, regardless of the operating temperature. The active microbial community in the mesophilic FBR was mainly composed of Clostridium and Ruminiclostridium at 37\u00a0\u00b0C. Thermophilic dark fermentation was shown to be suitable for treatment of high temperature, xylose-containing wastewaters, as it resulted in a higher energy output compared to the mesophilic counterpart.", "keywords": ["570", "116 Chemical sciences", "116", "01 natural sciences", "6. Clean water", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.ijhydene.2018.01.158"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Hydrogen%20Energy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijhydene.2018.01.158", "name": "item", "description": "10.1016/j.ijhydene.2018.01.158", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijhydene.2018.01.158"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-03-01T00:00:00Z"}}, {"id": "10.1016/j.ijhydene.2018.03.117", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:16:07Z", "type": "Journal Article", "created": "2018-04-18", "title": "Inoculum pretreatment differentially affects the active microbial community performing mesophilic and thermophilic dark fermentation of xylose", "description": "Abstract   The influence of different inoculum pretreatments (pH and temperature shocks) on mesophilic (37\u00a0\u00b0C) and thermophilic (55\u00a0\u00b0C) dark fermentative H2 production from xylose (50\u00a0mM) and, for the first time, on the composition of the active microbial community was evaluated. At 37\u00a0\u00b0C, an acidic shock (pH 3, 24\u00a0h) resulted in the highest yield of 0.8\u00a0mol H2 mol\u22121 xylose. The H2 and butyrate yield correlated with the relative abundance of Clostridiaceae in the mesophilic active microbial community, whereas Lactobacillaceae were the most abundant non-hydrogenic competitors according to RNA-based analysis. At 55\u00a0\u00b0C, Clostridium and Thermoanaerobacterium were linked to H2 production, but only an alkaline shock (pH 10, 24\u00a0h) repressed lactate production, resulting in the highest yield of 1.2\u00a0mol H2 mol\u22121 xylose. This study showed that pretreatments differentially affect the structure and productivity of the active mesophilic and thermophilic microbial community developed from an inoculum.", "keywords": ["Clostridium", "570", "Temperature shock", "Sustainability and the Environment", "116 Chemical sciences", "MiSeq", "Energy Engineering and Power Technology", "116", "02 engineering and technology", "Condensed Matter Physics", "01 natural sciences", "6. Clean water", "Lactobacillus", "Fuel Technology", "pH shock", "Renewable Energy", "0204 chemical engineering", "Biohydrogen", "Biohydrogen; Clostridium; Lactobacillus; MiSeq; pH shock; Temperature shock; Renewable Energy", " Sustainability and the Environment; Fuel Technology; Condensed Matter Physics; Energy Engineering and Power Technology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.ijhydene.2018.03.117"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Hydrogen%20Energy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijhydene.2018.03.117", "name": "item", "description": "10.1016/j.ijhydene.2018.03.117", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijhydene.2018.03.117"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-05-01T00:00:00Z"}}, {"id": "10.1016/j.ijhydene.2019.01.256", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:16:07Z", "type": "Journal Article", "created": "2019-02-28", "title": "Impacts of short-term temperature fluctuations on\u00a0biohydrogen production and resilience of\u00a0thermophilic microbial communities", "description": "Abstract   Anaerobic microflora enriched for dark fermentative H2 production from a mixture of glucose and xylose was used in batch cultivations to determine the effects of sudden short-term temperature fluctuations on H2 yield and microbial community composition. Batch cultures initially cultivated at 55\u00a0\u00b0C (control) were subjected to downward (from 55\u00a0\u00b0C to 35\u00a0\u00b0C or 45\u00a0\u00b0C) or upward (from 55\u00a0\u00b0C to 65\u00a0\u00b0C or 75\u00a0\u00b0C) temperature shifts for 48\u00a0h after which, each culture was transferred to a fresh medium and cultivated again at 55\u00a0\u00b0C for two consecutive batch cycles. The average H2 yield obtained during the first cultivation at 55\u00a0\u00b0C was 2.1\u00a0\u00b1\u00a00.14\u00a0mol H2 mol\u22121 hexose equivalent. During the temperature shifts, the obtained H2 yields were 1.8\u00a0\u00b1\u00a00.15, 1.6\u00a0\u00b1\u00a00.27 and 1.9\u00a0\u00b1\u00a00.00\u00a0mol H2 mol\u22121 hexose equivalent at 35\u00a0\u00b0C, 45\u00a0\u00b0C and 65\u00a0\u00b0C, respectively, while no metabolic activity was observed at 75\u00a0\u00b0C. The sugars were completely utilized during the 48\u00a0h temperature shift to 35\u00a0\u00b0C but not at 65\u00a0\u00b0C and 45\u00a0\u00b0C. At the end of the second cycle after the different temperature shifts, the H2 yield obtained was 96.5, 91.6, 79.9 and 54.1% (second cycle after temperature shift to 35\u00a0\u00b0C, 45\u00a0\u00b0C, 65\u00a0\u00b0C and 75\u00a0\u00b0C, respectively) when compared to the average H2 yield produced in the control at 55\u00a0\u00b0C. Characterization of the microbial communities present in the control culture at 55\u00a0\u00b0C showed the predominance of Thermoanaerobacteriales, Clostridiales and Bacilliales. The microbial community composition differed based on the fluctuation temperature with Thermoanaerobacteriales being most dominant during the upward temperature fluctuations and Clostridiales being the most dominant during the downward temperature fluctuations.", "keywords": ["[SDE] Environmental Sciences", "2. Zero hunger", "570", "660", "218 Environmental engineering", "[SDV]Life Sciences [q-bio]", "116 Chemical sciences", "temperature fluctuation", "116", "02 engineering and technology", "15. Life on land", "biological H-2 production", "01 natural sciences", "[SDV] Life Sciences [q-bio]", "recovery", "dark fermentation", "218", "[SDE]Environmental Sciences", "0204 chemical engineering", "resilience", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://hal.inrae.fr/hal-02623475/file/Manuscript-Okonkwo_Temp_IJHE.pdf"}, {"href": "https://doi.org/10.1016/j.ijhydene.2019.01.256"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Hydrogen%20Energy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijhydene.2019.01.256", "name": "item", "description": "10.1016/j.ijhydene.2019.01.256", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijhydene.2019.01.256"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-03-01T00:00:00Z"}}, {"id": "10.1016/j.procbio.2018.02.018", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-22T16:16:18Z", "type": "Journal Article", "created": "2018-02-23", "title": "Effect of N/S ratio on anoxic thiosulfate oxidation in a fluidized bed reactor: Experimental and artificial neural network model analysis", "description": "Abstract   Anoxic thiosulfate (S2O32\u2212) oxidation using autotrophic denitrification by a mixed culture of nitrate reducing, sulfur oxidizing bacteria (NR-SOB) was studied in a fluidized bed reactor (FBR). The long-term performance of the FBR was evaluated for 306 days at three nitrogen-to-sulfur (N/S) molar ratios (0.5, 0.3 and 0.1) and a hydraulic retention time (HRT) of 5\u202fh. S2O32\u2212 removal efficiencies >99% were obtained at a N/S ratio of 0.5 and a S2O32\u2212 and nitrate (NO3\u2212) loading rate of 820 (\u00b184) mg S-S2O32\u2212 L\u22121 d\u22121 and 173 (\u00b110) mg N-NO3\u2212 L\u22121 d\u22121, respectively. The S2O32\u2212 removal efficiency decreased to 76% and 26% at N/S ratios of 0.3 and 0.1, respectively, and recovered to 80% within 3 days after increasing the N/S ratio from 0.1 back to 0.5. The highest observed half-saturation (Ks) and inhibition (KI) constants of the biofilm-grown NR-SOB obtained from batch cultivations were 172 and 800\u202fmg S-S2O32\u2212 L\u22121, respectively. Thiobacilus denitrificans was the dominant microorganism in the FBR. Artificial neural network modeling successfully predicted S2O32\u2212 and NO3\u2212 removal efficiencies and SO42\u2212 production in the FBR. Additionally, results from the sensitivity analysis showed that the effluent pH was the most influential parameter affecting the S2O32\u2212 removal efficiency.", "keywords": ["116 Chemical sciences", "0211 other engineering and technologies", "116", "02 engineering and technology", "540", "01 natural sciences", "6. Clean water", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.procbio.2018.02.018"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Process%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.procbio.2018.02.018", "name": "item", "description": "10.1016/j.procbio.2018.02.018", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.procbio.2018.02.018"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-05-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2015.07.094", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:16:22Z", "type": "Journal Article", "created": "2015-08-15", "title": "Fighting Carbon Loss Of Degraded Peatlands By Jump-Starting Ecosystem Functioning With Ecological Restoration", "description": "Degradation of ecosystems is a great concern on the maintenance of biodiversity and ecosystem services. Ecological restoration fights degradation aiming at the recovery of ecosystem functions such as carbon (C) sequestration and ecosystem structures like plant communities responsible for the C sequestration function. We selected 38 pristine, drained and restored boreal peatland sites in Finland and asked i) what is the long-term effect of drainage on the peatland surface layer C storage, ii) can restoration recover ecosystem functioning (surface layer growth) and structure (plant community composition) and iii) is the recovery of the original structure needed for the recovery of ecosystem functions? We found that drainage had resulted in a substantial net loss of C from surface layer of drained sites. Restoration was successful in regaining natural growth rate in the peatland surface layer already within 5 years after restoration. However, the regenerated surface layer sequestered C at a mean rate of 116.3 g m(-2) yr(-1) (SE 12.7), when a comparable short-term rate was 178.2 g m(-2) yr(-1) (SE 13.3) at the pristine sites. The plant community compositions of the restored sites were considerably dissimilar to those of pristine sites still 10 years after restoration. We conclude that ecological restoration can be used to jump-start some key peatland ecosystem functions even without the recovery of original ecosystem structure (plant community composition). However, the re-establishment of other functions like C sequestration may require more profound recovery of conditions and ecosystem structure. We discuss the potential economic value of restored peatland ecosystems from the perspective of their C sequestration function.", "keywords": ["0301 basic medicine", "Carbon Sequestration", "Conservation of Natural Resources", "ecosystem structure\u2013function relationship", "Ecology and Evolutionary Biology", "ta1172", "Museo", "plant community composition", "turve", "03 medical and health sciences", "Museum", "ecosystem recovery", "Ekologia ja evoluutiobiologia", "ta116", "Ecosystem", "Environmental Restoration and Remediation", "Finland", "0303 health sciences", "hiilensidonta", "Biodiversity", "15. Life on land", "16. Peace & justice", "carbon sequestration", "Carbon", "13. Climate action", "Wetlands", "peat", "ta1181", "ecosystem degradation"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2015.07.094"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2015.07.094", "name": "item", "description": "10.1016/j.scitotenv.2015.07.094", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2015.07.094"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-12-01T00:00:00Z"}}, {"id": "10.1016/j.watres.2017.02.063", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-22T16:16:48Z", "type": "Journal Article", "created": "2017-02-28", "title": "Biohydrogen production from xylose by fresh and digested activated sludge at 37, 55 and 70\u00a0\u00b0C", "description": "Two heat-treated inocula, fresh and digested activated sludge from the same municipal wastewater treatment plant, were compared for their H2 production via dark fermentation at mesophilic (37\u00a0\u00b0C), thermophilic (55\u00a0\u00b0C) and hyperthermophilic (70\u00a0\u00b0C) conditions using xylose as the substrate. At both 37 and 55\u00a0\u00b0C, the fresh activated sludge yielded more H2 than the digested sludge, whereas at 70\u00a0\u00b0C, neither of the inocula produced H2 effectively. A maximum yield of 1.85\u00a0mol H2 per mol of xylose consumed was obtained at 55\u00a0\u00b0C. H2 production was linked to acetate and butyrate production, and there was a linear correlation (R2\u00a0=\u00a00.96) between the butyrate and H2 yield for the fresh activated sludge inoculum at 55\u00a0\u00b0C. Approximately 2.4\u00a0mol H2 per mol of butyrate produced were obtained against a theoretical maximum of 2.0, suggesting that H2 was produced via the acetate pathway prior to switching to the butyrate pathway due to the increased H2 partial pressure. Clostridia sp. were the prevalent species at both 37 and 55\u00a0\u00b0C, irrespectively of the inoculum type. Although the two inocula originated from the same plant, different thermophilic microorganisms were detected at 55\u00a0\u00b0C. Thermoanaerobacter sp., detected only in the fresh activated sludge cultures, may have contributed to the high H2 yield obtained with such an inoculum.", "keywords": ["Xylose", "Sewage", "116 Chemical sciences", "116", "02 engineering and technology", "01 natural sciences", "6. Clean water", "12. Responsible consumption", "Bioreactors", "13. Climate action", "Fermentation", "11. Sustainability", "0204 chemical engineering", "Hydrogen", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.watres.2017.02.063"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2017.02.063", "name": "item", "description": "10.1016/j.watres.2017.02.063", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2017.02.063"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-05-01T00:00:00Z"}}, {"id": "10.1039/c7ra12316g", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-22T16:17:11Z", "type": "Journal Article", "created": "2018-01-15", "title": "Composition and role of the attached and planktonic microbial communities in mesophilic and thermophilic xylose-fed microbial fuel cells", "description": "<p>A mesophilic (37 \u00b0C) and a thermophilic (55 \u00b0C) two-chamber microbial fuel cell (MFC) were studied and compared for their power production from xylose and the anode-attached, membrane-attached and planktonic microbial communities involved.</p>", "keywords": ["570", "waste-water", "116 Chemical sciences", "116", "bacterium", "01 natural sciences", "7. Clean energy", "biofilm", "3. Good health", "Chemistry", "sludge", "13. Climate action", "extracellular electron-transfer", "geobacter-sulfurreducens", "electricity-generation", "phylogenetic analyses", "enrichment culture", "performance", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://pubs.rsc.org/en/content/articlepdf/2018/RA/C7RA12316G"}, {"href": "https://doi.org/10.1039/c7ra12316g"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/RSC%20Advances", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1039/c7ra12316g", "name": "item", "description": "10.1039/c7ra12316g", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1039/c7ra12316g"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-01-01T00:00:00Z"}}, {"id": "10.1039/c9ew00220k", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:17:12Z", "type": "Journal Article", "created": "2019-04-29", "title": "Long-term performance evaluation of an anoxic sulfur oxidizing moving bed biofilm reactor under nitrate limited conditions", "description": "<p>An anoxic sulfur-oxidizing moving bed biofilm reactor (MBBR) treating sulfur and nitrate-contaminated synthetic wastewater was monitored for 306 days under feed nitrogen-to-sulfur (N/S) molar ratios of 0.5, 0.3 and 0.1.</p>", "keywords": ["Aurora Universities Network", "570", "Horizon 2020", "EC", "Environmental Engineering", "218 Environmental engineering", "116 Chemical sciences", "H2020", "116", "6. Clean water", "218", "European Joint Doctorates", "11. Sustainability", "European Commission", "Knowmad Institut", "Netherlands", "Water Science and Technology"]}, "links": [{"href": "http://pubs.rsc.org/en/content/articlepdf/2019/EW/C9EW00220K"}, {"href": "https://doi.org/10.1039/c9ew00220k"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%3A%20Water%20Research%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1039/c9ew00220k", "name": "item", "description": "10.1039/c9ew00220k", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1039/c9ew00220k"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-01-01T00:00:00Z"}}, {"id": "10.1093/ismeco/ycae116", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:17:34Z", "type": "Journal Article", "created": "2024-10-08", "title": "Land use effects on soil microbiome composition and traits with consequences for soil carbon cycling", "description": "Abstract                <p>The soil microbiome determines the fate of plant-fixed carbon. The shifts in soil properties caused by land use change leads to modifications in microbiome function, resulting in either loss or gain of soil organic carbon (SOC). Soil pH is the primary factor regulating microbiome characteristics leading to distinct pathways of microbial carbon cycling, but the underlying mechanisms remain understudied. Here, the taxa-trait relationships behind the variable fate of SOC were investigated using metaproteomics, metabarcoding, and a 13C-labeled litter decomposition experiment across two temperate sites with differing soil pH each with a paired land use intensity contrast. 13C incorporation into microbial biomass increased with land use intensification in low-pH soil but decreased in high-pH soil, with potential impact on carbon use efficiency in opposing directions. Reduction in biosynthesis traits was due to increased abundance of proteins linked to resource acquisition and stress tolerance. These trait trade-offs were underpinned by land use intensification-induced changes in dominant taxa with distinct traits. We observed divergent pH-controlled pathways of SOC cycling. In low-pH soil, land use intensification alleviates microbial abiotic stress resulting in increased biomass production but promotes decomposition and SOC loss. In contrast, in high-pH soil, land use intensification increases microbial physiological constraints and decreases biomass production, leading to reduced necromass build-up and SOC stabilization. We demonstrate how microbial biomass production and respiration dynamics and therefore carbon use efficiency can be decoupled from SOC highlighting the need for its careful consideration in managing SOC storage for soil health and climate change mitigation.</p", "keywords": ["soil health", "Supplementary Data", "QH301 Biology", "carbon use efficiency", "carbon cycling", "https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/ismecommun/4/1/10.1093_ismeco_ycae116/1/otu_table_16s_table_s1_ycae116.xlsx?Expires=1737538557&Signature=3IutEpMaJIknJFjSbheOQYWpAwXt2atlN4YtPR7BTaTGf3jrf1M6yHgYzlnrttKlwpbFcwz-IqYq96oubC5FxfBQQyiIC0H-az-D~Bkstxc9XHkEmERELO~nurTlszmUndzm3jLsKF05x00PNsiNFlGKUhlsMB6wRmyO3v3GNBqHQVdswXZ3UAjfXvqqinyDLK54UCxfLk8eKpcfFnvVctxQ8Hrk3gP-eMFToKDlXgPD4MXGrdegvcZblx6g8FAvJruLIG1NWIRJ6wzx6HcmAYiZDJcGosKrdjMBIznM8YIJjBrfWwhGvjh15Z7MJnsUWn8PjxLjXfww29q-YfQnw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA", "https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/ismecommun/4/1/10.1093_ismeco_ycae116/1/otu_table_18s_table_s2_ycae116.xlsx?Expires=1737538557&Signature=ZVWC9BaJ2MOsxOOfzrmd-9nuLAy5yHOmeqJQmKHhQ1z7mXxXITIYAvM8BpVkEkQHB7Bo-6dNEm5FlC6eAuTroyq-dvMW3PD6MNP9SN5KgwSrKUeHM6IKNhzav6Q4zd48B95IPreN5UKQTTVPrphpdOxfdVKYKxD3qOMdWqmHXt-IAD~W80PJ0BjvpHXPQ0pYCmGInVv1Fe-L3k~OKo80rD0xtncnBCFRd8DVHTIY5JLjJr4-E~M3Gainkbz2AVLZwys3S6MMEboS8vKSj~rG34Z04ByT6dBjp0XDj2H9K7WjXlEqOoPIwUWUUfcVvn4N5wZ6R6YFZr9mk4qTZKdEow__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA", "004", "soil organic carbon", "QH301", "soil pH", "13C labelling", "land use intensity", "soil microbiome", "metabarcoding", "SDG 13 - Climate Action", "metaproteomics", "Original Article", "SDG 15 - Life on Land"]}, "links": [{"href": "https://doi.org/10.1093/ismeco/ycae116"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ISME%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/ismeco/ycae116", "name": "item", "description": "10.1093/ismeco/ycae116", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/ismeco/ycae116"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "10.1155/2019/1751783", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-22T16:18:13Z", "type": "Journal Article", "created": "2019-05-02", "title": "Acetotrophic Activity Facilitates Methanogenesis from LCFA at Low Temperatures: Screening from Mesophilic Inocula", "description": "<p>The inoculum source plays a crucial role in the anaerobic treatment of wastewaters. Lipids are present in various wastewaters and have a high methanogenic potential, but their hydrolysis results in the production of long chain fatty acids (LCFAs) that are inhibitory to anaerobic microorganisms. Screening of inoculum for the anaerobic treatment of LCFA-containing wastewaters has been performed at mesophilic and thermophilic conditions. However, an evaluation of inocula for producing methane from LCFA-containing wastewater has not yet been conducted at low temperatures and needs to be undertaken. In this study, three inocula (one granular sludge and two municipal digester sludges) were assessed for methane production from LCFA-containing synthetic dairy wastewater (SDW) at low temperatures (10 and 20\uffc2\uffb0C). A methane yield (based on mL-CH4/g-CODadded) of 86-65% with acetate and 45-20% with SDW was achieved within 10 days using unacclimated granular sludge, whereas the municipal digester sludges produced methane only at 20\uffc2\uffb0C but not at 10\uffc2\uffb0C even after 200 days of incubation. The acetotrophic activity in the inoculum was found to be crucial for methane production from LCFA at low temperatures, highlighting the role ofMethanosaeta(acetoclastic archaea) at low temperatures. The presence of bacterial taxa from the familySyntrophaceae(Syntrophusand uncultured taxa) in the inoculum was found to be important for methane production from SDW at 10\uffc2\uffb0C. This study suggests the evaluation of acetotrophic activity and the initial microbial community characteristics by high-throughput amplicon sequencing for selecting the inoculum for producing methane at low temperatures (up to 10\uffc2\uffb0C) from lipid-containing wastewaters.</p>", "keywords": ["Deltaproteobacteria", "0301 basic medicine", "570", "0303 health sciences", "Sewage", "218 Environmental engineering", "Microbiota", "116 Chemical sciences", "Fatty Acids", "Temperature", "116", "Acetates", "Methanosarcinales", "6. Clean water", "03 medical and health sciences", "218", "13. Climate action", "11. Sustainability", "Anaerobiosis", "Methane", "Research Article"]}, "links": [{"href": "https://doi.org/10.1155/2019/1751783"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Archaea", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1155/2019/1751783", "name": "item", "description": "10.1155/2019/1751783", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1155/2019/1751783"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-02T00:00:00Z"}}, {"id": "11573/1419330", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-22T16:22:13Z", "type": "Journal Article", "created": "2020-06-05", "title": "Variability in pulmonary diffusing capacity in heart failure", "description": "As pulmonary diffusing capacity is related to mortality risk and prognosis in patients with heart failure (HF), it is measured frequently. As such, it would be essential to know the week-to-week variability (reproducibility) of pulmonary diffusing capacity for carbon monoxide (DLCO) and nitric oxide (DLNO). This variability would let clinicians understand what a clinically measurable change in DLCO and DLNO would be in these patients.On three different days spanning over ten weeks, 40\u2009H\u2009F patients underwent testing for DLCO and DLNO. DLCO was determined after a 4\u2009s and 10\u2009s breath-hold maneuver, while DLNO was determined after a 4\u2009s breath-hold maneuver.Forty heart failure patients (66\u2009\u00b1\u200910 years; BMI\u2009=\u200928.4\u2009\u00b1\u20094.6\u2009kg\u2219m-2; 28 males), that were referred to our clinic were able to complete the protocol. DLCO (4\u2009s breath-hold) and DLNO (4\u2009s breath-hold) were 79\u2009\u00b1\u200919 % and 59\u2009\u00b1\u200914 % predicted, respectively. Fifty percent of patients (n\u2009=\u200920) were below the lower limit of normal (LLN, below the 5th percentile) for predicted DLCO (4\u2009s), while 78 % of patients (n\u2009=\u200931) were below the LLN for predicted DLNO. All 16 patients that were below the LLN for DLCO were also below the LLN for DLNO. Over a ten week period, the reproducibility of DLNO (4\u2009s) DLCO (4\u2009s) and DLCO (10\u2009s) was 18.9, 8.2, and 5.9\u2009mL\u2009min\u2009mmHg-1, respectively.The week-to-week fluctuation in DLNO (4\u2009s), as a percentage, is less than DLCO (4\u2009s) in patients with HF. The reproducibility of DLNO in patients with HF is like that of healthy subjects.", "keywords": ["Male", "DLCO; DLNO; lung function; heart failure; reproducibility", "Physiology (science-metrix)", "Heart Disease (rcdc)", "Pulmonary Diffusing Capacity (mesh)", "3208 Medical physiology (for-2020)", "Heart failure", "Nitric Oxide", "Lung (rcdc)", "DLCO", "DLCO; DLNO; Heart failure; Lung function; Reproducibility;", "Clinical Research (rcdc)", "03 medical and health sciences", "0302 clinical medicine", "1102 Cardiorespiratory Medicine and Haematology (for)", "Middle Aged (mesh)", "Reproducibility of Results (mesh)", "Humans", "32 Biomedical and Clinical Sciences (for-2020)", "Male (mesh)", "3202 Clinical Sciences (for-2020)", "Carbon Monoxide (mesh)", "Aged", "DLNO", "Heart Failure", "Humans (mesh)", "Carbon Monoxide", "Cardiovascular (hrcs-hc)", "Aged (mesh)", "3201 Cardiovascular medicine and haematology (for-2020)", "Reproducibility of Results", "Heart Failure (mesh)", "Middle Aged", "1109 Neurosciences (for)", "Lung function", "Reproducibility", "3. Good health", "1116 Medical Physiology (for)", "4.2 Evaluation of markers and technologies (hrcs-rac)", "Female (mesh)", "Nitric Oxide (mesh)", "Pulmonary Diffusing Capacity", "Cardiovascular (rcdc)", "Female"]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/743296/2/agostoni%203.pdf"}, {"href": "https://doi.org/11573/1419330"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Respiratory%20Physiology%20%26amp%3B%20Neurobiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11573/1419330", "name": "item", "description": "11573/1419330", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11573/1419330"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-09-01T00:00:00Z"}}, {"id": "2164/24787", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:22:40Z", "type": "Journal Article", "created": "2024-10-08", "title": "Land use effects on soil microbiome composition and traits with consequences for soil carbon cycling", "description": "Abstract                <p>The soil microbiome determines the fate of plant-fixed carbon. The shifts in soil properties caused by land use change leads to modifications in microbiome function, resulting in either loss or gain of soil organic carbon (SOC). Soil pH is the primary factor regulating microbiome characteristics leading to distinct pathways of microbial carbon cycling, but the underlying mechanisms remain understudied. Here, the taxa-trait relationships behind the variable fate of SOC were investigated using metaproteomics, metabarcoding, and a 13C-labeled litter decomposition experiment across two temperate sites with differing soil pH each with a paired land use intensity contrast. 13C incorporation into microbial biomass increased with land use intensification in low-pH soil but decreased in high-pH soil, with potential impact on carbon use efficiency in opposing directions. Reduction in biosynthesis traits was due to increased abundance of proteins linked to resource acquisition and stress tolerance. These trait trade-offs were underpinned by land use intensification-induced changes in dominant taxa with distinct traits. We observed divergent pH-controlled pathways of SOC cycling. In low-pH soil, land use intensification alleviates microbial abiotic stress resulting in increased biomass production but promotes decomposition and SOC loss. In contrast, in high-pH soil, land use intensification increases microbial physiological constraints and decreases biomass production, leading to reduced necromass build-up and SOC stabilization. We demonstrate how microbial biomass production and respiration dynamics and therefore carbon use efficiency can be decoupled from SOC highlighting the need for its careful consideration in managing SOC storage for soil health and climate change mitigation.</p", "keywords": ["soil health", "Supplementary Data", "QH301 Biology", "carbon use efficiency", "carbon cycling", "https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/ismecommun/4/1/10.1093_ismeco_ycae116/1/otu_table_16s_table_s1_ycae116.xlsx?Expires=1737538557&Signature=3IutEpMaJIknJFjSbheOQYWpAwXt2atlN4YtPR7BTaTGf3jrf1M6yHgYzlnrttKlwpbFcwz-IqYq96oubC5FxfBQQyiIC0H-az-D~Bkstxc9XHkEmERELO~nurTlszmUndzm3jLsKF05x00PNsiNFlGKUhlsMB6wRmyO3v3GNBqHQVdswXZ3UAjfXvqqinyDLK54UCxfLk8eKpcfFnvVctxQ8Hrk3gP-eMFToKDlXgPD4MXGrdegvcZblx6g8FAvJruLIG1NWIRJ6wzx6HcmAYiZDJcGosKrdjMBIznM8YIJjBrfWwhGvjh15Z7MJnsUWn8PjxLjXfww29q-YfQnw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA", "https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/ismecommun/4/1/10.1093_ismeco_ycae116/1/otu_table_18s_table_s2_ycae116.xlsx?Expires=1737538557&Signature=ZVWC9BaJ2MOsxOOfzrmd-9nuLAy5yHOmeqJQmKHhQ1z7mXxXITIYAvM8BpVkEkQHB7Bo-6dNEm5FlC6eAuTroyq-dvMW3PD6MNP9SN5KgwSrKUeHM6IKNhzav6Q4zd48B95IPreN5UKQTTVPrphpdOxfdVKYKxD3qOMdWqmHXt-IAD~W80PJ0BjvpHXPQ0pYCmGInVv1Fe-L3k~OKo80rD0xtncnBCFRd8DVHTIY5JLjJr4-E~M3Gainkbz2AVLZwys3S6MMEboS8vKSj~rG34Z04ByT6dBjp0XDj2H9K7WjXlEqOoPIwUWUUfcVvn4N5wZ6R6YFZr9mk4qTZKdEow__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA", "004", "soil organic carbon", "QH301", "soil pH", "13C labelling", "land use intensity", "soil microbiome", "metabarcoding", "SDG 13 - Climate Action", "metaproteomics", "Original Article", "SDG 15 - Life on Land"]}, "links": [{"href": "https://doi.org/2164/24787"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ISME%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2164/24787", "name": "item", "description": "2164/24787", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2164/24787"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "37499875", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-22T16:23:17Z", "type": "Journal Article", "created": "2023-07-25", "title": "Fast and reliable method to estimate global DNA methylation in plants and fungi with high-pressure liquid chromatography (HPLC)-ultraviolet detection and even more sensitive one with HPLC-mass spectrometry", "description": "DNA (Deoxyribonucleic acid) methylation is one of the epigenetic modifications of DNA, acting as a bridge between genotype and phenotype. Thus, disruption of DNA methylation pattern has tremendous consequences for organism development. Current methods to determine DNA methylation suffer from methodological drawbacks like high requirement of DNA and poor reproducibility of chromatograms. Here we provide a fast and reliable method using high-pressure liquid chromatography (HPLC)-ultraviolet (UV) detector and even more sensitive one with HPLC- mass spectrometry (MS) and we test this method with various plant and fungal DNA isolates. We optimized the preparation of the DNA degradation step to decrease background noise, we improved separation conditions to provide reliable and reproducible chromatograms and conditions to measure nucleotides in HPLC-MS. We showed that global DNA methylation level can be accurately and reproducibly measured with as little as 0.2\u00a0\u00b5M for HPLC-UV and 0.02\u00a0\u00b5M for HPLC-MS of methylated cytosine.", "keywords": ["Plant DNA", "DNA methylation", "ta1183", "ta1182", "Fungi", "610", "Reproducibility of Results", "DNA Methylation", "Mass Spectrometry", "Fungal DNA", "chromatography", "DNA", " Fungal", "ta116", "Chromatography", " High Pressure Liquid"]}, "links": [{"href": "https://doi.org/37499875"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "37499875", "name": "item", "description": "37499875", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/37499875"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-09-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=116&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=116&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=116&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=116&offset=25", "hreflang": "en-US"}], "numberMatched": 25, "numberReturned": 25, "distributedFeatures": [], "timeStamp": "2026-09-22T22:51:27.487283Z"}