Our findings indicate the importance of paleoclimatic information to improve quantitative predictions of global soil C stocks.
Despite having key functions in terrestrial ecosystems, information on the dominant soil fungi and their ecological preferences at the global scale is lacking. To fill this knowledge gap, we surveyed 235 soils from across the globe. Our findings indicate that 83 phylotypes (<0.1% of the retrieved fungi), mostly belonging to wind dispersed, generalist Ascomycota, dominate soils globally. We identify patterns and ecological drivers of dominant soil fungal taxa occurrence, and present a map of their distribution in soils worldwide. Whole-genome comparisons with less dominant, generalist fungi point at a significantly higher number of genes related to stress-tolerance and resource uptake in the dominant fungi, suggesting that they might be better in colonising a wide range of environments. Our findings constitute a major advance in our understanding of the ecology of fungi, and have implications for the development of strategies to preserve them and the ecosystem functions they provide.Identifying the global drivers of soil priming is essential to understanding C cycling in terrestrial ecosystems. We conducted a survey of soils across 86 globally-distributed locations, spanning a wide range of climates, biotic communities, and soil conditions, and evaluated the apparent soil priming effect using13C-glucose labeling. Here we show that the magnitude of the positive apparent priming effect (increase in CO2release through accelerated microbial biomass turnover) was negatively associated with SOC content and microbial respiration rates. Our statistical modeling suggests that apparent priming effects tend to be negative in more mesic sites associated with higher SOC contents. In contrast, a single-input of labile C causes positive apparent priming effects in more arid locations with low SOC contents. Our results provide solid evidence that SOC content plays a critical role in regulating apparent priming effects, with important implications for the improvement of C cycling models under global change scenarios. Our findings indicate the importance of paleoclimatic information to improve quantitative predictions of global soil C stocks.
Receptors that distinguish the multitude of microbes surrounding plants in the environment enable dynamic responses to the biotic and abiotic conditions encountered. In this study, we identify and characterise a glycan receptor kinase, EPR3a, closely related to the exopolysaccharide receptor EPR3. Epr3a is up-regulated in roots colonised by arbuscular mycorrhizal (AM) fungi and is able to bind glucans with a branching pattern characteristic of surface-exposed fungal glucans. Expression studies with cellular resolution show localised activation of the Epr3a promoter in cortical root cells containing arbuscules. Fungal infection and intracellular arbuscule formation are reduced in epr3a mutants. In vitro, the EPR3a ectodomain binds cell wall glucans in affinity gel electrophoresis assays. In microscale thermophoresis (MST) assays, rhizobial exopolysaccharide binding is detected with affinities comparable to those observed for EPR3, and both EPR3a and EPR3 bind a well-defined \uffce\uffb2-1,3/\uffce\uffb2-1,6 decasaccharide derived from exopolysaccharides of endophytic and pathogenic fungi. Both EPR3a and EPR3 function in the intracellular accommodation of microbes. However, contrasting expression patterns and divergent ligand affinities result in distinct functions in AM colonisation and rhizobial infection in Lotus japonicus. The presence of Epr3a and Epr3 genes in both eudicot and monocot plant genomes suggest a conserved function of these receptor kinases in glycan perception.Nitrous oxide (N2O) emissions from grazing animal excreta are estimated to be responsible for 1.5 Tg of the total 6.7 Tg of anthropogenic N2O emissions. This study was conducted to determine the in situ effect of incorporating biochar, into soil, on N2O emissions from bovine urine patches and associated pasture uptake of N. The effects of biochar rate (0\uffe2\uff80\uff9330 t ha\uffe2\uff88\uff921), following soil incorporation, were investigated on ruminant urine\uffe2\uff80\uff90derived N2O fluxes, N uptake by pasture, and pasture yield. During an 86\uffe2\uff80\uff90d spring\uffe2\uff80\uff90summer period, where irrigation and rainfall occurred, the N2O fluxes from 15N labeled ruminant urine patches were reduced by >50%, after incorporating 30 t ha\uffe2\uff88\uff921 of biochar. Taking into account the N2O emissions from the control plots, 30 t ha\uffe2\uff88\uff921 of biochar reduced the N2O emission factor from urine by 70%. The atom% 15N enrichment of the N2O emitted was lower in the 30 t ha\uffe2\uff88\uff921 biochar treatment, indicating less urine\uffe2\uff80\uff90N contributed to the N2O flux. Soil NO3\uffe2\uff88\uff92\uffe2\uff80\uff90N concentrations were lower with increasing biochar rate during the first 30 d following urine deposition. No differences occurred, due to biochar addition, with respect to dry matter yields, herbage N content, or recovery of 15N applied in herbage. Incorporating biochar into the soil can significantly diminish ruminant urine\uffe2\uff80\uff90derived N2O emissions. Further work is required to determine the persistence of the observed effect and to fully understand the mechanism(s) of the observed reduction in N2O fluxes.
", "keywords": ["bovine urine", "550", "Nitrogen", "Nitrous Oxide", "Urine", "Soil", "ANZSRC::0702 Animal Production", "ANZSRC::0503 Soil Sciences", "Animals", "Humans", "biochar", "Weather", "2. Zero hunger", "nitrous oxide", "ANZSRC::31 Biological sciences", "emissions", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "ANZSRC::050304 Soil Chemistry (excl. Carbon Sequestration Science)", "ANZSRC::37 Earth sciences", "13. Climate action", "Charcoal", "ANZSRC::41 Environmental sciences", "0401 agriculture", " forestry", " and fisheries", "Cattle", "Volatilization"]}, "links": [{"href": "https://doi.org/10.2134/jeq2010.0419"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2010.0419", "name": "item", "description": "10.2134/jeq2010.0419", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2010.0419"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-03-01T00:00:00Z"}}, {"id": "10.2136/sssaj2009.0185", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:25Z", "type": "Journal Article", "created": "2010-04-22", "title": "Unweathered Wood Biochar Impact On Nitrous Oxide Emissions From A Bovine-Urine-Amended Pasture Soil", "description": "Low\uffe2\uff80\uff90temperature pyrolysis of biomass produces a product known as biochar The incorporation of this material into the soil has been advocated as a C sequestration method. Biochar also has the potential to influence the soil N cycle by altering nitrification rates and by adsorbing or NH3 Biochar can be incorporated into the soil during renovation of intensively managed pasture soils. These managed pastures are a significant source of N2O, a greenhouse gas, produced in ruminant urine patches. We hypothesized that biochar effects on the N cycle could reduce the soil inorganic\uffe2\uff80\uff90N pool available for N2O\uffe2\uff80\uff90producing mechanisms. A laboratory study was performed to examine the effect of biochar incorporation into soil (20 Mg ha\uffe2\uff88\uff921) on N2O\uffe2\uff80\uff90N and NH3\uffe2\uff80\uff93N fluxes, and inorganic\uffe2\uff80\uff90N transformations, following the application of bovine urine (760 kg N ha\uffe2\uff88\uff921). Treatments included controls (soil only and soil plus biochar), and two urine treatments (soil plus urine and soil plus biochar plus urine). Fluxes of N2O from the biochar plus urine treatment were generally higher than from urine alone during the first 30 d, but after 50 d there was no significant difference (P = 0.11) in terms of cumulative N2O\uffe2\uff80\uff90N emitted as a percentage of the urine N applied during the 53\uffe2\uff80\uff90d period; however, NH3\uffe2\uff80\uff93N fluxes were enhanced by approximately 3% of the N applied in the biochar plus urine treatment compared with the urine\uffe2\uff80\uff90only treatment after 17 d. Soil inorganic\uffe2\uff80\uff90N pools differed between treatments, with higher concentrations in the presence of biochar, indicative of lower rates of nitrification. The inorganic\uffe2\uff80\uff90N pool available for N2O\uffe2\uff80\uff90producing mechanisms was not reduced, however, by adding biochar.
", "keywords": ["2. Zero hunger", "bovine urine", "550", "ANZSRC::31 Biological sciences", "soil nitrogen", "ANZSRC::30 Agricultural", "04 agricultural and veterinary sciences", "15. Life on land", "630", "6. Clean water", "veterinary and food sciences", "Marsden::300103 Soil chemistry", "13. Climate action", "ANZSRC::41 Environmental sciences", "0401 agriculture", " forestry", " and fisheries", "biochar"]}, "links": [{"href": "https://doi.org/10.2136/sssaj2009.0185"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Science%20Society%20of%20America%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2136/sssaj2009.0185", "name": "item", "description": "10.2136/sssaj2009.0185", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2136/sssaj2009.0185"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-05-01T00:00:00Z"}}, {"id": "10449/84375", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:24:49Z", "type": "Journal Article", "created": "2024-02-26", "title": "Class-wide genomic tendency throughout specific extremes in black fungi", "description": "Open AccessThe classes Dothideomycetes and Eurotiomycetes include constitutively melanized fungi adapted to extreme conditions and they are widely distributed in diverse hostile habitats worldwide. Yet, despite the growing interest in these fungi, there is a considerable gap of knowledge on their functionality. Their genomic analysis is still in its infancy and the possibility to understand their adaptive strategies and exploit their potentialities in bioremediation is very limited. Here, we supply a genome catalog of 118 black fungi, encompassing diferent ecologies, phylogenies and lifestyles, as a frst example of a comparative genomic study at high level of diversity. Results indicate that, as a rule, Dothideomycetes show more variable genome size and that larger genomes are associated with harshest conditions; low temperature tolerance and DNA repair capacity are overrepresented in their genomes. In Eurotiomycetes high temperature tolerance and capacity to metabolize hydrocarbons are more frequently present and these abilities are positively correlated with the human presence. The genomic features are consistent with the prevalent ecologies in the two classes. Indeed, Dothideomycetes are more common in cold and dry environments with high capacity for DNA repair being consistent with the normally highly UV-impacted conditions in their habitats; in contrast, Eurotiomycetes spread mainly in hot human-impacted sites with industrial pollution. Mean annual temperature and isothermality are positively correlated with tolerance to high temperatures in Dothideomycetes, suggesting that, despite their preference for the cold, they are potentially equipped to survive even when temperatures rise due to the global warming.", "keywords": ["570", "Black Fungi", "Plant Biology", "Health Disparities (rcdc)", "Mycology & Parasitology", "Biotechnology (rcdc)", "Microbiology", "3105 Genetics (for-2020)", "0607 Plant Biology (for)", "0605 Microbiology (for)", "Genetics", "Black fungi \u00b7 Stress resistance \u00b7 Comparative genomics \u00b7 Extreme environments", "14. Life underwater", "3108 Plant biology (for-2020)", "2. Zero hunger", "Evolutionary Biology", "31 Biological Sciences (for-2020)", "Black fungi", "Genetics (rcdc)", "3107 Microbiology (for-2020)", "Comparative genomics", "Human Genome", "500", "Mycology & Parasitology (science-metrix)", "3104 Evolutionary biology (for-2020)", "Extreme environments", "Biological Sciences", "15. Life on land", "Human Genome (rcdc)", "3. Good health", "Health Disparities", "0603 Evolutionary Biology (for)", "13. Climate action", "8. Economic growth", "Stress resistance", "Settore BIO/19 - MICROBIOLOGIA GENERALE", "Biotechnology"]}, "links": [{"href": "https://iris.unitn.it/bitstream/11572/450837/1/FUDI_Coleine%20et%20al%20v2..pdf"}, {"href": "https://iris.unitn.it/bitstream/11572/450837/3/s13225-024-00533-y.pdf"}, {"href": "https://escholarship.org/content/qt86f967px/qt86f967px.pdf"}, {"href": "https://doi.org/10449/84375"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Fungal%20Diversity", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10449/84375", "name": "item", "description": "10449/84375", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10449/84375"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-02-26T00:00:00Z"}}, {"id": "10182/16842", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:24:38Z", "type": "Journal Article", "created": "2023-05-18", "title": "A glycan receptor kinase facilitates intracellular accommodation of arbuscular mycorrhiza and symbiotic rhizobia in the legume Lotus japonicus", "description": "Receptors that distinguish the multitude of microbes surrounding plants in the environment enable dynamic responses to the biotic and abiotic conditions encountered. In this study, we identify and characterise a glycan receptor kinase, EPR3a, closely related to the exopolysaccharide receptor EPR3. Epr3a is up-regulated in roots colonised by arbuscular mycorrhizal (AM) fungi and is able to bind glucans with a branching pattern characteristic of surface-exposed fungal glucans. Expression studies with cellular resolution show localised activation of the Epr3a promoter in cortical root cells containing arbuscules. Fungal infection and intracellular arbuscule formation are reduced in epr3a mutants. In vitro, the EPR3a ectodomain binds cell wall glucans in affinity gel electrophoresis assays. In microscale thermophoresis (MST) assays, rhizobial exopolysaccharide binding is detected with affinities comparable to those observed for EPR3, and both EPR3a and EPR3 bind a well-defined \uffce\uffb2-1,3/\uffce\uffb2-1,6 decasaccharide derived from exopolysaccharides of endophytic and pathogenic fungi. Both EPR3a and EPR3 function in the intracellular accommodation of microbes. However, contrasting expression patterns and divergent ligand affinities result in distinct functions in AM colonisation and rhizobial infection in Lotus japonicus. The presence of Epr3a and Epr3 genes in both eudicot and monocot plant genomes suggest a conserved function of these receptor kinases in glycan perception. Abstract. Measuring in situ soil fluxes of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) continuously at high frequency requires appropriate technology. We tested the combination of a commercial automated soil CO2 flux chamber system (LI-8100A) with a CH4 and N2O analyzer (Picarro G2308) in a tropical rainforest for 4\u00a0months. A chamber closure time of 2\u2009min was sufficient for a reliable estimation of CO2 and CH4 fluxes (100\u2009% and 98.5\u2009% of fluxes were above minimum detectable flux \u2013 MDF, respectively). This closure time was generally not suitable for a reliable estimation of the low N2O fluxes in this ecosystem but was sufficient for detecting rare major peak events. A closure time of 25\u2009min was more appropriate for reliable estimation of most N2O fluxes (85.6\u2009% of measured fluxes are above MDF\u2009\u00b1\u20090.002\u2009nmol\u2009m\u22122\u2009s\u22121). Our study highlights the importance of adjusted closure time for each gas.
Research in global change ecology relies heavily on global climatic grids derived from estimates of air temperature in open areas at around 2\uffc2\uffa0m above the ground. These climatic grids do not reflect conditions below vegetation canopies and near the ground surface, where critical ecosystem functions occur and most terrestrial species reside. Here, we provide global maps of soil temperature and bioclimatic variables at a 1\uffe2\uff80\uff90km2resolution for 0\uffe2\uff80\uff935 and 5\uffe2\uff80\uff9315\uffc2\uffa0cm soil depth. These maps were created by calculating the difference (i.e. offset) between in situ soil temperature measurements, based on time series from over 1200 1\uffe2\uff80\uff90km2pixels (summarized from 8519 unique temperature sensors) across all the world's major terrestrial biomes, and coarse\uffe2\uff80\uff90grained air temperature estimates from ERA5\uffe2\uff80\uff90Land (an atmospheric reanalysis by the European Centre for Medium\uffe2\uff80\uff90Range Weather Forecasts). We show that mean annual soil temperature differs markedly from the corresponding gridded air temperature, by up to 10\uffc2\uffb0C (mean\uffc2\uffa0=\uffc2\uffa03.0\uffc2\uffa0\uffc2\uffb1\uffc2\uffa02.1\uffc2\uffb0C), with substantial variation across biomes and seasons. Over the year, soils in cold and/or dry biomes are substantially warmer (+3.6\uffc2\uffa0\uffc2\uffb1\uffc2\uffa02.3\uffc2\uffb0C) than gridded air temperature, whereas soils in warm and humid environments are on average slightly cooler (\uffe2\uff88\uff920.7\uffc2\uffa0\uffc2\uffb1\uffc2\uffa02.3\uffc2\uffb0C). The observed substantial and biome\uffe2\uff80\uff90specific offsets emphasize that the projected impacts of climate and climate change on near\uffe2\uff80\uff90surface biodiversity and ecosystem functioning are inaccurately assessed when air rather than soil temperature is used, especially in cold environments. The global soil\uffe2\uff80\uff90related bioclimatic variables provided here are an important step forward for any application in ecology and related disciplines. Nevertheless, we highlight the need to fill remaining geographic gaps by collecting more in situ measurements of microclimate conditions to further enhance the spatiotemporal resolution of global soil temperature products for ecological applications. Our findings indicate the importance of paleoclimatic information to improve quantitative predictions of global soil C stocks.
Despite having key functions in terrestrial ecosystems, information on the dominant soil fungi and their ecological preferences at the global scale is lacking. To fill this knowledge gap, we surveyed 235 soils from across the globe. Our findings indicate that 83 phylotypes (<0.1% of the retrieved fungi), mostly belonging to wind dispersed, generalist Ascomycota, dominate soils globally. We identify patterns and ecological drivers of dominant soil fungal taxa occurrence, and present a map of their distribution in soils worldwide. Whole-genome comparisons with less dominant, generalist fungi point at a significantly higher number of genes related to stress-tolerance and resource uptake in the dominant fungi, suggesting that they might be better in colonising a wide range of environments. Our findings constitute a major advance in our understanding of the ecology of fungi, and have implications for the development of strategies to preserve them and the ecosystem functions they provide.Identifying the global drivers of soil priming is essential to understanding C cycling in terrestrial ecosystems. We conducted a survey of soils across 86 globally-distributed locations, spanning a wide range of climates, biotic communities, and soil conditions, and evaluated the apparent soil priming effect using13C-glucose labeling. Here we show that the magnitude of the positive apparent priming effect (increase in CO2release through accelerated microbial biomass turnover) was negatively associated with SOC content and microbial respiration rates. Our statistical modeling suggests that apparent priming effects tend to be negative in more mesic sites associated with higher SOC contents. In contrast, a single-input of labile C causes positive apparent priming effects in more arid locations with low SOC contents. Our results provide solid evidence that SOC content plays a critical role in regulating apparent priming effects, with important implications for the improvement of C cycling models under global change scenarios.Rock-dwelling microorganisms are key players in ecosystem functioning of Antarctic ice free-areas. Yet, little is known about their diversity and ecology. Here, we performed metagenomic analyses on rocks from across Antarctica comprising >75,000 viral operational taxonomic units (vOTUS). We found largely undescribed, highly diverse and spatially structured virus communities potentially influencing bacterial adaptation and biogeochemistry. This catalog lays the foundation for expanding knowledge of the virosphere in extreme environments.