{"type": "FeatureCollection", "features": [{"id": "10.1007/s11104-011-0870-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-26T16:16:14Z", "type": "Journal Article", "created": "2011-07-05", "title": "Biochar Adsorbed Ammonia Is Bioavailable", "description": "Biochar is produced as a by-product of the low temperature pyrolysis of biomass during bioenergy extraction and its incorporation into soil is of global interest as a potential carbon sequestration tool. Biochar influences soil nitrogen transformations and its capacity to take up ammonia is well recognized. Anthropogenic emissions of ammonia need to be mitigated due to negative environmental impacts and economic losses. Here we use an isotope of nitrogen to show that ammonia-N adsorbed by biochar is stable in ambient air, but readily bioavailable when placed in the soil. When biochars, containing adsorbed 15N labelled ammonia, were incorporated into soil the 15N recovery by roots averaged 6.8% but ranged from 26.1% to 10.9% in leaf tissue due to differing biochar properties with plant 15N recovery greater when acidic biochars were used to capture ammonia. Recovery of 15N as total soil nitrogen (organic+inorganic) ranged from 45% to 29% of 15N applied. We provide a proof of concept for a synergistic mitigation option where anthropogenic ammonia emissions could be captured using biochar, and made bioavailable in soils, thus leading to nitrogen capture by crops, while simultaneously sequestering carbon in soils.", "keywords": ["ryegrass", "550", "ANZSRC::31 Biological sciences", "ANZSRC::0703 Crop and Pasture Production", "ANZSRC::050301 Carbon Sequestration Science", "ANZSRC::30 Agricultural", "04 agricultural and veterinary sciences", "540", "ammonia", "01 natural sciences", "7. Clean energy", "nitrogen", "veterinary and food sciences", "13. Climate action", "ANZSRC::0503 Soil Sciences", "ANZSRC::41 Environmental sciences", "0401 agriculture", " forestry", " and fisheries", "biochar", "ANZSRC::070304 Crop and Pasture Biomass and Bioproducts", "N stable isotope", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s11104-011-0870-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11104-011-0870-3", "name": "item", "description": "10.1007/s11104-011-0870-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11104-011-0870-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-07-06T00:00:00Z"}}, {"id": "10.1016/j.agee.2014.02.021", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-26T16:16:42Z", "type": "Journal Article", "created": "2014-03-15", "title": "Biochar Does Not Affect Soil N-Transformations Or Microbial Community Structure Under Ruminant Urine Patches But Does Alter Relative Proportions Of Nitrogen Cycling Bacteria", "description": "Abstract Nitrogen (N) cycling, especially denitrification, can be significantly altered when biochar is used as a soil conditioner. These alterations in N-cycling have been attributed to a combination of physicochemical change, alterations in microbial community ecology and pervading climatic conditions. This study investigated seasonal bacterial community change over two years in combination with a short-term winter study of N-transformations under bovine urine patches. A silt-loam pastoral soil in Canterbury, New Zealand was amended with either 0, 15 or 30\u00a0t\u00a0ha \u22121 of Pinus radiata biochar (pyrolysed at \u223c450\u00a0\u00b0C) and bovine urine was added to patches within the 0 and 30\u00a0t\u00a0ha \u22121 biochar amended plots (designated as 0\u00a0U and 30\u00a0U treatments, where U indicates \u2018urine\u2019). No discernible differences in bacterial community structure were observed during the two year study or the short term N-transformation study when comparing non-amended and biochar-amended soil. Differences in bacterial community structure were only evident when comparing seasons, with data pertaining to each season from successive years clustering together. During the short-term N-transformation study, bacterial communities formed 3 distinct clusters corresponding to elevated levels of urine derived NH 4 + -N (days 0\u201310), increases in NO 3 \u2212 -N and N 2 O (days 10\u201322) and a decline in NO 3 \u2212 -N and N 2 O (day 20 onward). Biochar amendment did increase the relative abundance of up to 50% of individual operational taxonomic units (OTUs or \u2018species\u2019), including key nitrite oxidisers and nitrate reducers. Biochar amendment did not affect the concentrations of inorganic-N compounds. The nir S (nitrite reductase) gene became elevated in the 30\u00a0U treatment relative to the 0\u00a0U treatment \u223c10 days after the initial urine application. The nos Z (nitrous oxide reductase) gene became elevated in the 30\u00a0U plots during the latter part of the experiment. Conclusions: \u2022 Biochar did not have a significant impact on the microbial community structure in pastoral soil over the course of two years. \u2022 The relative proportion of nitrifiers and denitrifiers increased in biochar amended soils subjected to large influxes of urine derived N. \u2022 Differences in N-transformation dynamics in the presence of biochar during the winter months were not statistically significant.", "keywords": ["2. Zero hunger", "N\u2082O emissions", "570", "denitrification", "bovine urine", "silt-loam soil", "ANZSRC::30 Agricultural", "04 agricultural and veterinary sciences", "15. Life on land", "soil microbial ecology", "winter", "nitrification", "630", "6. Clean water", "veterinary and food sciences", "T-RFLP", "new generation sequencing", "13. Climate action", "ANZSRC::41 Environmental sciences", "XXXXXX - Unknown", "0401 agriculture", " forestry", " and fisheries", "biochar", "ANZSRC::44 Human society"], "contacts": [{"organization": "Timothy J. Clough, Kelly Hamonts, Leo M. Condron, Craig Anderson, Craig Anderson,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.agee.2014.02.021"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2014.02.021", "name": "item", "description": "10.1016/j.agee.2014.02.021", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2014.02.021"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-06-01T00:00:00Z"}}, {"id": "10.1016/j.apsoil.2006.05.001", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-26T16:16:55Z", "type": "Journal Article", "created": "2006-06-19", "title": "Influence Of Organic And Mineral Amendments On Microbial Soil Properties And Processes", "description": "Abstract Microbial diversity in soils is considered important for maintaining sustainability of agricultural production systems. However, the links between microbial diversity and ecosystem processes are not well understood. This study was designed to gain better understanding of the effects of short-term management practices on the microbial community and how changes in the microbial community affect key soil processes. The effects of different forms of nitrogen (N) on soil biology and N dynamics was determined in two soils with organic and conventional management histories that varied in soil microbial properties but had the same fertility. The soils were amended with equal amounts of N (100\u00a0kg\u00a0ha\u22121) in organic (lupin, Lupinus angustifolius L.) and mineral form (urea), respectively. Over a 91-day period, microbial biomass C and N, dehydrogenase enzyme activity, community structure of pseudomondas (sensu stricto), actinomycetes and \u03b1 proteobacteria (by denaturing gradient gel electrophoresis (DGGE) following PCR amplification of 16S rDNA fragments) and N mineralisation were measured. Lupin amendment resulted in a two- to five-fold increase in microbial biomass and enzyme activity, while these parameters did not differ significantly between the urea and control treatments. The PCR\u2013DGGE analysis showed that the addition of mineral and organic compounds had an influence on the microbial community composition in the short term (up to 10 days) but the effects were not sustained over the 91-day incubation period. Microbial community structure was strongly influenced by the presence or lack of substrate, while the type of amendment (organic or mineral) had an effect on microbial biomass size and activity. These findings show that the addition of green manures improved soil biology by increasing microbial biomass and activity irrespective of management history, that no direct relationship existed among microbial structure, enzyme activity and N mineralisation, and that microbial community structure (by PCR\u2013DGGE) was more strongly influenced by inherent soil and environmental factors than by short-term management practices.", "keywords": ["2. Zero hunger", "570", "ANZSRC::31 Biological sciences", "nitrogen mineralisation", "urea", "ANZSRC::30 Agricultural", "04 agricultural and veterinary sciences", "15. Life on land", "630", "6. Clean water", "lupin (Lupinus angustifolius L.) green manure", "Marsden::300102 Soil biology", "veterinary and food sciences", "microbial community structure", "13. Climate action", "ANZSRC::41 Environmental sciences", "0401 agriculture", " forestry", " and fisheries", "DGGE", "organic and conventional farming practices"]}, "links": [{"href": "https://doi.org/10.1016/j.apsoil.2006.05.001"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Soil%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.apsoil.2006.05.001", "name": "item", "description": "10.1016/j.apsoil.2006.05.001", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.apsoil.2006.05.001"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-01-01T00:00:00Z"}}, {"id": "10.1371/journal.pbio.3002127", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-26T16:21:36Z", "type": "Journal Article", "created": "2023-05-18", "title": "A glycan receptor kinase facilitates intracellular accommodation of arbuscular mycorrhiza and symbiotic rhizobia in the legume Lotus japonicus", "description": "

Receptors that distinguish the multitude of microbes surrounding plants in the environment enable dynamic responses to the biotic and abiotic conditions encountered. In this study, we identify and characterise a glycan receptor kinase, EPR3a, closely related to the exopolysaccharide receptor EPR3. Epr3a is up-regulated in roots colonised by arbuscular mycorrhizal (AM) fungi and is able to bind glucans with a branching pattern characteristic of surface-exposed fungal glucans. Expression studies with cellular resolution show localised activation of the Epr3a promoter in cortical root cells containing arbuscules. Fungal infection and intracellular arbuscule formation are reduced in epr3a mutants. In vitro, the EPR3a ectodomain binds cell wall glucans in affinity gel electrophoresis assays. In microscale thermophoresis (MST) assays, rhizobial exopolysaccharide binding is detected with affinities comparable to those observed for EPR3, and both EPR3a and EPR3 bind a well-defined \uffce\uffb2-1,3/\uffce\uffb2-1,6 decasaccharide derived from exopolysaccharides of endophytic and pathogenic fungi. Both EPR3a and EPR3 function in the intracellular accommodation of microbes. However, contrasting expression patterns and divergent ligand affinities result in distinct functions in AM colonisation and rhizobial infection in Lotus japonicus. The presence of Epr3a and Epr3 genes in both eudicot and monocot plant genomes suggest a conserved function of these receptor kinases in glycan perception.Low\uffe2\uff80\uff90temperature pyrolysis of biomass produces a product known as biochar The incorporation of this material into the soil has been advocated as a C sequestration method. Biochar also has the potential to influence the soil N cycle by altering nitrification rates and by adsorbing or NH3 Biochar can be incorporated into the soil during renovation of intensively managed pasture soils. These managed pastures are a significant source of N2O, a greenhouse gas, produced in ruminant urine patches. We hypothesized that biochar effects on the N cycle could reduce the soil inorganic\uffe2\uff80\uff90N pool available for N2O\uffe2\uff80\uff90producing mechanisms. A laboratory study was performed to examine the effect of biochar incorporation into soil (20 Mg ha\uffe2\uff88\uff921) on N2O\uffe2\uff80\uff90N and NH3\uffe2\uff80\uff93N fluxes, and inorganic\uffe2\uff80\uff90N transformations, following the application of bovine urine (760 kg N ha\uffe2\uff88\uff921). Treatments included controls (soil only and soil plus biochar), and two urine treatments (soil plus urine and soil plus biochar plus urine). Fluxes of N2O from the biochar plus urine treatment were generally higher than from urine alone during the first 30 d, but after 50 d there was no significant difference (P = 0.11) in terms of cumulative N2O\uffe2\uff80\uff90N emitted as a percentage of the urine N applied during the 53\uffe2\uff80\uff90d period; however, NH3\uffe2\uff80\uff93N fluxes were enhanced by approximately 3% of the N applied in the biochar plus urine treatment compared with the urine\uffe2\uff80\uff90only treatment after 17 d. Soil inorganic\uffe2\uff80\uff90N pools differed between treatments, with higher concentrations in the presence of biochar, indicative of lower rates of nitrification. The inorganic\uffe2\uff80\uff90N pool available for N2O\uffe2\uff80\uff90producing mechanisms was not reduced, however, by adding biochar.

", "keywords": ["2. Zero hunger", "bovine urine", "550", "ANZSRC::31 Biological sciences", "soil nitrogen", "ANZSRC::30 Agricultural", "04 agricultural and veterinary sciences", "15. Life on land", "630", "6. Clean water", "veterinary and food sciences", "Marsden::300103 Soil chemistry", "13. Climate action", "ANZSRC::41 Environmental sciences", "0401 agriculture", " forestry", " and fisheries", "biochar"]}, "links": [{"href": "https://doi.org/10.2136/sssaj2009.0185"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Science%20Society%20of%20America%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2136/sssaj2009.0185", "name": "item", "description": "10.2136/sssaj2009.0185", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2136/sssaj2009.0185"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-05-01T00:00:00Z"}}, {"id": "10182/16842", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-26T16:28:08Z", "type": "Journal Article", "created": "2023-05-18", "title": "A glycan receptor kinase facilitates intracellular accommodation of arbuscular mycorrhiza and symbiotic rhizobia in the legume Lotus japonicus", "description": "

Receptors that distinguish the multitude of microbes surrounding plants in the environment enable dynamic responses to the biotic and abiotic conditions encountered. In this study, we identify and characterise a glycan receptor kinase, EPR3a, closely related to the exopolysaccharide receptor EPR3. Epr3a is up-regulated in roots colonised by arbuscular mycorrhizal (AM) fungi and is able to bind glucans with a branching pattern characteristic of surface-exposed fungal glucans. Expression studies with cellular resolution show localised activation of the Epr3a promoter in cortical root cells containing arbuscules. Fungal infection and intracellular arbuscule formation are reduced in epr3a mutants. In vitro, the EPR3a ectodomain binds cell wall glucans in affinity gel electrophoresis assays. In microscale thermophoresis (MST) assays, rhizobial exopolysaccharide binding is detected with affinities comparable to those observed for EPR3, and both EPR3a and EPR3 bind a well-defined \uffce\uffb2-1,3/\uffce\uffb2-1,6 decasaccharide derived from exopolysaccharides of endophytic and pathogenic fungi. Both EPR3a and EPR3 function in the intracellular accommodation of microbes. However, contrasting expression patterns and divergent ligand affinities result in distinct functions in AM colonisation and rhizobial infection in Lotus japonicus. The presence of Epr3a and Epr3 genes in both eudicot and monocot plant genomes suggest a conserved function of these receptor kinases in glycan perception.