Anthropogenic changes in biodiversity and atmospheric temperature significantly influence ecosystem processes. However, little is known about potential interactive effects of plant diversity and warming on essential ecosystem properties, such as soil microbial functions and element cycling. We studied the effects of orthogonal manipulations of plant diversity (one, four, and 16 species) and warming (ambient, +1.5\uffc2\uffb0C, and +3\uffc2\uffb0C) on soil microbial biomass, respiration, growth after nutrient additions, and activities of extracellular enzymes in 2011 and 2012 in the BAC (biodiversity and climate) perennial grassland experiment site at Cedar Creek, Minnesota, USA. Focal enzymes are involved in essential biogeochemical processes of the carbon, nitrogen, and phosphorus cycles. Soil microbial biomass and some enzyme activities involved in the C and N cycle increased significantly with increasing plant diversity in both years. In addition, 16\uffe2\uff80\uff90species mixtures buffered warming induced reductions in topsoil water content. We found no interactive effects of plant diversity and warming on soil microbial biomass and growth rates. However, the activity of several enzymes (1,4\uffe2\uff80\uff90\uffce\uffb2\uffe2\uff80\uff90glucosidase, 1,4\uffe2\uff80\uff90\uffce\uffb2\uffe2\uff80\uff90N\uffe2\uff80\uff90acetylglucosaminidase, phosphatase, peroxidase) depended on interactions between plant diversity and warming with elevated activities of enzymes involved in the C, N, and P cycles at both high plant diversity and high warming levels. Increasing plant diversity consistently decreased microbial biomass\uffe2\uff80\uff90specific enzyme activities and altered soil microbial growth responses to nutrient additions, indicating that plant diversity changed nutrient limitations and/or microbial community composition. In contrast to our expectations, higher plant diversity only buffered temperature effects on soil water content, but not on microbial functions. Temperature effects on some soil enzymes were greatest at high plant diversity. In total, our results suggest that the fundamental temperature ranges of soil microbial communities may be sufficiently broad to buffer their functioning against changes in temperature and that plant diversity may be a dominant control of soil microbial processes in a changing world.
", "keywords": ["aboveground-belowground interactions", "Hot Temperature", "warming", "Climate Change", "biodiversity-ecosystem functioning", "global warming", "soil microbial ecology", "Soil", "XXXXXX - Unknown", "Biomass", "global change", "Soil Microbiology", "2. Zero hunger", "microbial biomass", "grasslands", "extracellular enzymes", "Biodiversity", "04 agricultural and veterinary sciences", "Plants", "15. Life on land", "plant diversity", "Enzymes", "grassland ecosystem", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "ecosystems"]}, "links": [{"href": "https://doi.org/10.1890/14-0088.1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1890/14-0088.1", "name": "item", "description": "10.1890/14-0088.1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1890/14-0088.1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-01-01T00:00:00Z"}}, {"id": "10.3897/rio.5.e34564", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:10Z", "type": "Journal Article", "created": "2019-04-01", "title": "Ecosystem responses to exotic earthworm invasion in northern North American forests", "description": "Earth is experiencing a substantial loss of biodiversity at the global scale, while both species gains and losses are occurring at local and regional scales. The influence of these nonrandom changes in species distributions could profoundly affect the functioning of ecosystems and the essential services that they provide. However, few experimental tests have been conducted examining the influence of species invasions on ecosystem functioning. Even fewer have been conducted using invasive ecosystem engineers, which can have disproportionately strong influence on native ecosystems relative to their own biomass. The invasion of exotic earthworms is a prime example of an ecosystem engineer that is influencing many ecosystems around the world. In particular, European earthworm invasions of northern North American forests cause simultaneous species gains and losses with significant consequences for essential ecosystem processes like nutrient cycling and crucial services to humanity like soil erosion control and carbon sequestration. Exotic earthworms are expected to select for specific traits in communities of soil microorganisms (fast-growing bacteria species), soil fauna (promoting the bacterial energy channel), and plants (graminoids) through direct and indirect effects. This will accelerate some ecosystem processes and decelerate others, fundamentally altering how invaded forests function. This project aims to investigate ecosystem responses of northern North American forests to earthworm invasion. Using a novel, synthetic combination of field observations, field experiments, lab experiments, and meta-analyses, the proposed work will be the first systematic examination of earthworm effects on (1) plant communities and (2) soil food webs and processes. Further, (3) effects of a changing climate (warming and reduced summer precipitation) on earthworm performance will be investigated in a unique field experiment designed to predict the future spread and consequences of earthworm invasion in North America. By assessing the soil chemical and physical properties as well as the taxonomic (e.g., by the latest next-generation sequencing techniques) and functional composition of plant, soil microbial and animal communities and the processes they drive in four forests, work packages I-III take complementary approaches to derive a comprehensive and generalizable picture of how ecosystems change in response to earthworm invasion. Finally, in work package IV, meta-analyses will be used to integrate the information from work packages I-III and existing literature to investigate if earthworms cause invasion waves, invasion meltdowns, habitat homogenization, and ecosystem state shifts. Global data will be synthesized to test if the relative magnitude of effects differs from place to place depending on the functional dissimilarity between native soil fauna and exotic earthworms. Moving from local to global scale, the present proposal examines the influence of earthworm invasions on biodiversity\uffe2\uff80\uff93ecosystem functioning relationships from an aboveground\uffe2\uff80\uff93belowground perspective in natural settings. This approach is highly innovative as it utilizes the invasion by exotic earthworms as an exciting model system that links invasion biology with trait-based community ecology, global change research, and ecosystem ecology, pioneering a new generation of biodiversity\uffe2\uff80\uff93ecosystem functioning research.
", "keywords": ["0106 biological sciences", "2. Zero hunger", "Science", "biodiversity-ecosystem functioning", "Q", "Aboveground-belowground interactions", "earthworms", "soil food webs", "15. Life on land", "invasion", "biodivers", "01 natural sciences", "plant communities", "biodiversity change", "13. Climate action", "11. Sustainability", "Lumbricidae", "global change"]}, "links": [{"href": "https://doi.org/10.3897/rio.5.e34564"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Research%20Ideas%20and%20Outcomes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3897/rio.5.e34564", "name": "item", "description": "10.3897/rio.5.e34564", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3897/rio.5.e34564"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-01T00:00:00Z"}}, {"id": "10.5061/dryad.f542t16", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:22:27Z", "type": "Dataset", "title": "Data from: Grazing and resource availability control soil nematode body size and abundance-mass relationship in semi-arid grassland", "description": "unspecified1. Body size is a central functional trait in ecological communities. Despite recognition of the importance of above-belowground interactions, effects of aboveground herbivores on size and abundance-size relationships in soil fauna are almost uncharted. Depending on climate and soil properties, herbivores may increase basal resources of soil food webs, or reduce pore space, mechanisms expected to have contrasting effects on soil animal body size. 2. We investigated how body size and shape of soil nematodes responded to mammalian grazers in three semi-arid grassland sites, along a gradient of soil texture and organic matter (OM) in a long-term herbivore removal study. We analysed nematode mass, length, diameter, body size distribution, and biomass distribution. We formulated two mechanistic hypotheses to assess whether resource availability or pore space was the dominant abiotic control and modulated the effects of grazing. 3. In ungrazed soils, average and maximum nematode size, as well as abundance and biomass of large nematodes, were greater in the high-OM than in the low-OM soil, and intermediate in the medium-OM soil. Grazing promoted larger sizes in the low-OM soil, where it had been shown to increase organic matter and microbial biomass, and led to more homogeneous average size and body size distribution across sites. The results support the hypothesis that nematode size was controlled by basal resource availability rather than by pore space. However, body shape might have been constrained by small pores in the fine-texture, high-OM soil, where nematodes were more elongated. 4. Grazing may facilitate larger sizes in soil nematode communities by boosting basal resources where these are limiting, with important implications for estimations of nematode biomass and contribution to carbon and nutrient cycling. These findings contribute to the insofar-limited mechanistic understanding of how herbivores can shape functional traits of soil fauna, and demonstrate that animals at one trophic level may control patterns in body size and abundance-size relationships in other trophic levels without a direct predator-prey or competitive linkage between them.", "keywords": ["2. Zero hunger", "Individual size distribution", "Soil texture", "Aboveground-belowground interactions", "15. Life on land", "Mammalian herbivores", "organic matter", "Soil fauna"], "contacts": [{"organization": "Andriuzzi, Walter S., Wall, Diana H.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.f542t16"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.f542t16", "name": "item", "description": "10.5061/dryad.f542t16", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.f542t16"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-17T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Aboveground-belowground+interactions&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Aboveground-belowground+interactions&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Aboveground-belowground+interactions&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Aboveground-belowground+interactions&offset=4", "hreflang": "en-US"}], "numberMatched": 4, "numberReturned": 4, "distributedFeatures": [], "timeStamp": "2026-05-31T02:21:51.302788Z"}