Ammonia-oxidizing archaea (AOA) are among the most numerous living organisms on Earth, and they play a pivotal role in the global biogeochemical nitrogen cycle. Despite this, little is known about the physiology and metabolism of AOA.
Long\uffe2\uff80\uff90term effects of elevated atmospheric CO2 on the ammonia\uffe2\uff80\uff90oxidizing and denitrifying bacteria in a grassland soil were investigated to test whether a shift in abundance of these N\uffe2\uff80\uff90cycling microorganisms was responsible for enhanced N2O emissions under elevated atmospheric CO2. Soil samples (7.5\uffc2\uffa0cm increments to 45\uffc2\uffa0cm depth) were collected in 2008 from the University of Giessen Free Air Carbon dioxide Enrichment (GiFACE), a permanent grassland exposed to moderately elevated atmospheric CO2 (+20%) since 1998. GiFACE plots lay on a soil moisture gradient because of gradually changing depth to the underlying water table and labeled as the DRY block (furthest from water table), MED block (intermediate to water table), and WET block (nearest to water table). Mean N2O emissions measured since 1998 have been significantly higher under elevated CO2. This study sought to identify microbial and biochemical parameters that might explain higher N2O emissions under elevated CO2. Soil biochemical parameters [extractable organic carbon (EOC), dissolved organic nitrogen (DON), NH4+, NO3\uffe2\uff88\uff92], and abundances of genes encoding the key enzymes involved in ammonia oxidation (amoA) and denitrification (nirK, nirS, nosZ) depended more on soil depth and block (underlying soil moisture gradient) than on elevated CO2. Ammonia oxidation and denitrification gene abundances, relative abundances (ratios) of nirS to nirK, of nosZ to both nirS and to nirK, and of the measured soil biochemical properties DON and NO3\uffe2\uff88\uff92 tended to be lower in elevated CO2 plots as compared with ambient plots in the MED and WET blocks while the DRY block exhibited an opposite trend. High N2O emissions under elevated CO2 in the MED and WET blocks correlated with lower nosZ to nirK ratios, suggesting that increased N2O emissions under elevated CO2 might be caused by a higher proportion of N2O\uffe2\uff80\uff90producing rather than N2O consuming (N2 producing) denitrifiers.
", "keywords": ["nirS", "2. Zero hunger", "N2O emissions", "denitrification", "[SDE.MCG]Environmental Sciences/Global Changes", "04 agricultural and veterinary sciences", "15. Life on land", "AOA", "6. Clean water", "AOB", "soil", "Enrichissement en gaz carbonique", "[SDE.MCG] Environmental Sciences/Global Changes", "Concentration \u00e9lev\u00e9e en CO2", "nosZ", "FACE", "13. Climate action", "ammonia oxidation", "nirK", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2486.2011.02470.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2011.02470.x", "name": "item", "description": "10.1111/j.1365-2486.2011.02470.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2011.02470.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-07-10T00:00:00Z"}}, {"id": "10.1128/aem.02470-21", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:19:55Z", "type": "Journal Article", "created": "2022-04-06", "title": "Hydrazines as Substrates and Inhibitors of the Archaeal Ammonia Oxidation Pathway", "description": "Ammonia-oxidizing archaea (AOA) are among the most numerous living organisms on Earth, and they play a pivotal role in the global biogeochemical nitrogen cycle. Despite this, little is known about the physiology and metabolism of AOA.
The effects of mineral fertilizer (NPK) and organic manure on the community structure of soil ammonia-oxidizing bacteria (AOB) was investigated in a long-term (16-year) fertilizer experiment. The experiment included seven treatments: organic manure, half organic manure N plus half fertilizer N, fertilizer NPK, fertilizer NP, fertilizer NK, fertilizer PK, and the control (without fertilization). N fertilization greatly increased soil nitrification potential, and mineral N fertilizer had a greater impact than organic manure, while N deficiency treatment (PK) had no significant effect. AOB community structure was analyzed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) of the amoA gene, which encodes the \uffce\uffb1 subunit of ammonia monooxygenase. DGGE profiles showed that the AOB community was more diverse in N-fertilized treatments than in the PK-fertilized treatment or the control, while one dominant band observed in the control could not be detected in any of the fertilized treatments. Phylogenetic analysis showed that the DGGE bands derived from N-fertilized treatments belonged to Nitrosospira cluster 3, indicating that N fertilization resulted in the dominance of Nitrosospira cluster 3 in soil. These results demonstrate that long-term application of N fertilizers could result in increased soil nitrification potential and the AOB community shifts in soil. Our results also showed the different effects of mineral fertilizer N versus organic manure N; the effects of P and K on the soil AOB community; and the importance of balanced fertilization with N, P, and K in promoting nitrification functions in arable soils.
", "keywords": ["2. Zero hunger", "Minerals", "Bacteria", "Nitrogen", "Molecular Sequence Data", "Sequence Analysis", " DNA", "04 agricultural and veterinary sciences", "6. Clean water", "Manure", "Ammonia", "0401 agriculture", " forestry", " and fisheries", "Fertilizers", "Oxidoreductases", "Ecosystem", "Gammaproteobacteria", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1128/aem.01536-06"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20and%20Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/aem.01536-06", "name": "item", "description": "10.1128/aem.01536-06", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/aem.01536-06"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-01-15T00:00:00Z"}}, {"id": "10.1128/msystems.00562-19", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:56Z", "type": "Journal Article", "created": "2020-01-13", "title": "Transcriptomic Response of Nitrosomonas europaea Transitioned from Ammonia- to Oxygen-Limited Steady-State Growth", "description": "Nitrification is a ubiquitous microbially mediated process in the environment and an essential process in engineered systems such as wastewater and drinking water treatment plants. However, nitrification also contributes to fertilizer loss from agricultural environments, increasing the eutrophication of downstream aquatic ecosystems, and produces the greenhouse gas nitrous oxide. As ammonia-oxidizing bacteria are the most dominant ammonia-oxidizing microbes in fertilized agricultural soils, understanding their responses to a variety of environmental conditions is essential for curbing the negative environmental effects of nitrification. Notably, oxygen limitation has been reported to significantly increase nitric oxide and nitrous oxide production during nitrification. Here, we investigate the physiology of the best-characterized ammonia-oxidizing bacterium, Nitrosomonas europaea , growing under oxygen-limited conditions.
", "keywords": ["OXIDIZING BACTERIUM", "0301 basic medicine", "nitrificatio", "Nitrosomonas europaea", "ammonia and oxygen limitation", "NITRIFICATION", "Microbiology", "CYTOCHROME-C", "03 medical and health sciences", "NITROUS-OXIDE PRODUCTION", "SDG 13 - Climate Action", "COMPLETE GENOME SEQUENCE", "ELECTRON-TRANSFER", "14. Life underwater", "SDG 2 \u2013 Kein Hunger", "SDG 2 - Zero Hunger", "Ammonia-oxidizing bacteria", "2. Zero hunger", "106022 Mikrobiologie", "chemostat", "0303 health sciences", "NITRIC-OXIDE", "N2O-PRODUCING PATHWAYS", "15. Life on land", "Ammonia and oxygen limitation", "Nitrification", "HYDROXYLAMINE OXIDOREDUCTASE", "nitrification", "QR1-502", "6. Clean water", "Chemostat", "13. Climate action", "SDG 13 \u2013 Ma\u00dfnahmen zum Klimaschutz", "ammonia-oxidizing bacteria", "106022 Microbiology", "Transcriptome", "transcriptome", "NO REDUCTASE-ACTIVITY", "COMPLETE NITRIFICATION", "Research Article"]}, "links": [{"href": "https://www.biorxiv.org/content/10.1101/765727v1.full.pdf"}, {"href": "https://journals.asm.org/doi/pdf/10.1128/mSystems.00562-19"}, {"href": "https://doi.org/10.1128/msystems.00562-19"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mSystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/msystems.00562-19", "name": "item", "description": "10.1128/msystems.00562-19", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/msystems.00562-19"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-09-11T00:00:00Z"}}, {"id": "10.1128/aem.02388-19", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:19:55Z", "type": "Journal Article", "created": "2020-02-20", "title": "Inhibition of Ammonia Monooxygenase from Ammonia-Oxidizing Archaea by Linear and Aromatic Alkynes", "description": "Archaeal and bacterial ammonia oxidizers (AOA and AOB, respectively) initiate nitrification by oxidizing ammonia to hydroxylamine, a reaction catalyzed by ammonia monooxygenase (AMO). AMO enzyme is difficult to purify in its active form, and its structure and biochemistry remain largely unexplored. The bacterial AMO and the closely related particulate methane monooxygenase (pMMO) have a broad range of hydrocarbon cooxidation substrates. This study provides insights into the AMO of previously unstudied archaeal genera, by comparing the response of the archaeal AMO, a bacterial AMO, and pMMO to inhibition by linear 1-alkynes and the aromatic alkyne, phenylacetylene. Reduced sensitivity to inhibition by larger alkynes suggests that the archaeal AMO has a narrower hydrocarbon substrate range than the bacterial AMO, as previously reported for other genera of AOA. Phenylacetylene inhibited the archaeal and bacterial AMOs at different thresholds and by different mechanisms of inhibition, highlighting structural differences between the two forms of monooxygenase.
This study was undertaken to examine the effects of forest fire on two important groups of N-cycling bacteria in soil, the nitrogen-fixing and ammonia-oxidizing bacteria. Sequence and terminal restriction fragment length polymorphism (T-RFLP) analysis of nifH and amoA PCR amplicons was performed on DNA samples from unburned, moderately burned, and severely burned soils of a mixed conifer forest. PCR results indicated that the soil biomass and proportion of nitrogen-fixing and ammonia-oxidizing species was less in soil from the fire-impacted sites than from the unburned sites. The number of dominant nifH sequence types was greater in fire-impacted soils, and nifH sequences that were most closely related to those from the spore-forming taxa Clostridium and Paenibacillus were more abundant in the burned soils. In T-RFLP patterns of the ammonia-oxidizing community, terminal restriction fragments (TRFs) representing amoA cluster 1, 2, or 4 Nitrosospira spp. were dominant (80 to 90%) in unburned soils, while TRFs representing amoA cluster 3A Nitrosospira spp. dominated (65 to 95%) in fire-impacted soils. The dominance of amoA cluster 3A Nitrosospira spp. sequence types was positively correlated with soil pH (5.6 to 7.5) and NH 3 -N levels (0.002 to 0.976 ppm), both of which were higher in burned soils. The decreased microbial biomass and shift in nitrogen-fixing and ammonia-oxidizing communities were still evident in fire-impacted soils collected 14 months after the fire.
", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Bacteria", "Base Sequence", "Molecular Sequence Data", "15. Life on land", "Polymerase Chain Reaction", "Fires", "Trees", "Soil", "03 medical and health sciences", "Ammonia", "Nitrogen Fixation", "Oxidoreductases", "Oxidation-Reduction", "Polymorphism", " Restriction Fragment Length", "Soil Microbiology"], "contacts": [{"organization": "Chris M. Yeager, Diana E. Northup, Susan M. Barns, Cheryl R. Kuske, Christy C. Grow,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1128/aem.71.5.2713-2722.2005"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20and%20Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/aem.71.5.2713-2722.2005", "name": "item", "description": "10.1128/aem.71.5.2713-2722.2005", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/aem.71.5.2713-2722.2005"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-05-01T00:00:00Z"}}, {"id": "10.2134/jeq2017.03.0106", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:58Z", "type": "Journal Article", "created": "2017-08-03", "title": "Combining Urease And Nitrification Inhibitors With Incorporation Reduces Ammonia And Nitrous Oxide Emissions And Increases Corn Yields", "description": "Less than 50% of applied nitrogen (N) fertilizer is typically recovered by corn (Zea mays L.) due to climatic constraints, soil degradation, overapplication, and losses to air and water. Two application methods, two N sources, and two inhibitors were evaluated to reduce N losses and enhance crop uptake. The treatments included broadcast urea (BrUrea), BrUrea with a urease inhibitor (BrUrea+UI), BrUrea with a urease and a nitrification inhibitor (BrUrea+UI+NI), injection of urea ammonium nitrate (InjUAN), and injected with one or both inhibitors (InjUAN+UI, InjUAN+UI+NI), and a control. The BrUrea treatment lost 50% (64.4 kg N ha\uffe2\uff88\uff921) of the applied N due to ammonia volatilization, but losses were reduced by 64% with BrUrea+UI+NI (23.0 kg N ha\uffe2\uff88\uff921) and by 60% with InjUAN (26.1 kg N ha\uffe2\uff88\uff921). Ammonia losses were lower and crop yields were greater in 2014 than 2013 as a result of the more favorable weather when N was applied in 2014. When ammonia volatilization was reduced by adding a urease inhibitor, N2O emissions were increased by 30 to 31% with BrUrea+UI and InjUAN+UI compared with BrUrea and InjUAN, respectively. Pollution swapping was avoided when both inhibitors were used (BrUrea+UI+NI, InjUAN+UI+NI) as both ammonia volatilization and N2O emissions were reduced, and corn grain yields increased by 5% with BrUrea+UI+NI and by 7% with InjUAN+UI+NI compared with BrUrea and InjUAN, respectively. The combination of two N management strategies (InjUAN+UI+NI) increased yields by 19% (12.9 t ha\uffe2\uff88\uff921) compared with BrUrea (10.8 t ha\uffe2\uff88\uff921).
Core Ideas
Ammonia volatilization resulted in 50% loss of applied urea over 2 yr.
When urease inhibitors were added with urea, ammonia volatilization was reduced by 64%.
Injection of UAN reduced ammonia volatilization by 60% compared with broadcast urea.
N2O emissions were increased by 30 to 31% when urease inhibitors were applied.
Pollution swapping was avoided when both urease and nitrification inhibitors were used.
", "keywords": ["2. Zero hunger", "Ammonia", "13. Climate action", "Denitrification", "Nitrous Oxide", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "Fertilizers", "Nitrification", "Urease", "Zea mays", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.2134/jeq2017.03.0106"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2017.03.0106", "name": "item", "description": "10.2134/jeq2017.03.0106", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2017.03.0106"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-09-01T00:00:00Z"}}, {"id": "10.3390/iecag2021-10017", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:21:43Z", "type": "Journal Article", "created": "2021-12-28", "title": "Application of Dairy Manure Amended with Mineral Fertilizer on Stubble-Covered Soil: Effects on Ammonia Emissions", "description": "Open AccessPresented at the 1st International Electronic Conference on Agronomy, 3\u201317 May 2021", "keywords": ["2. Zero hunger", "13. Climate action", "manure", "no-tillage", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "ammonia", "nitrogen", "6. Clean water", "organic-mineral fertilizer"]}, "links": [{"href": "https://www.mdpi.com/2673-9976/3/1/19/pdf"}, {"href": "https://doi.org/10.3390/iecag2021-10017"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%201st%20International%20Electronic%20Conference%20on%20Agronomy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/iecag2021-10017", "name": "item", "description": "10.3390/iecag2021-10017", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/iecag2021-10017"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-05-11T00:00:00Z"}}, {"id": "10.13227/j.hjkx.2016.10.039", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:12Z", "type": "Journal Article", "description": "A pot experiment was conducted to study the effect of straw returning and domestic sewage irrigation on the dynamics of NH4+-N concentration and pH in the flood water, and ammonia volatilization of paddy fields. The results showed that the NH4+-N concentration in flood water was significantly increased by wheat straw returning while significantly decreased by domestic sewage irrigation. The cumulative ammonia volatilization in the whole rice season under tap water irrigation and straw removal treatment was 58.29 kg\u00b7hm-2, accounting for 24.29% of the total N applied. The N loss ratio of ammonia volatilization was significantly increased to 45.66% by wheat straw returning, while significantly decreased to 17.26% under straw removal and 32.72% under straw returning by domestic sewage irrigation. Significant positive interaction was observed between straw incorporation and domestic sewage irrigation on ammonia volatilization loss. The average N loss from ammonia volatilization during the tillering stage was the highest among the three fertilization stages, accounting for 7.38%-24.44% of the total N applied. In addition, ammonia volatilization fluxes showed a significant positive correlation with the flood water NH4+-N concentration, irrespective of the irrigation water, but had no significant correlation with pH. These results indicated that straw returning increased ammonia volatilization losses, whereas domestic sewage irrigation could effectively reduce ammonia volatilization losses and simultaneously replace 44.41% of chemical nitrogen fertilizer by the N contained in the domestic sewage. The combination of domestic sewage irrigation and straw returning would be an ecological and environmental-friendly measure for rice nitrogen management in Taihu Lake region.", "keywords": ["Soil", "Agricultural Irrigation", "Plant Stems", "Sewage", "Ammonia", "Nitrogen", "Oryza", "Volatilization", "Fertilizers", "01 natural sciences", "Water Pollutants", " Chemical", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Li-Hong Xue, Li-Hui Fan, Lin-Zhang Yang, Ganghua Li, Peng-Fu Hou, Shaohua Wang, Shan-Shan Xu,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.13227/j.hjkx.2016.10.039"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Huan%20jing%20ke%20xue%3D%20Huanjing%20kexue", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.13227/j.hjkx.2016.10.039", "name": "item", "description": "10.13227/j.hjkx.2016.10.039", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.13227/j.hjkx.2016.10.039"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-10-01T00:00:00Z"}}, {"id": "10.1371/journal.pone.0070569", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:14Z", "type": "Journal Article", "created": "2013-08-02", "title": "Effects Of Controlled-Release Fertiliser On Nitrogen Use Efficiency In Summer Maize", "description": "Nitrogen (N) is a nutrient element necessary for plant growth and development. However, excessive inputs of N will lead to inefficient use and large N losses to the environment, which can adversely affect air and water quality, biodiversity and human health. To examine the effects of controlled-release fertilisers (CRF) on yield, we measured ammonia volatilisation, N use efficiency (NUE) and photosynthetic rate after anthesis in summer maize hybrid cultivar Zhengdan958. Maize was grown using common compound fertiliser (CCF), the same amount of resin-coated controlled release fertiliser (CRFIII), the same amount of sulphur-coated controlled release fertiliser (SCFIII) as CCF, 75% CRF (CRFII) and SCF (SCFII), 50% CRF (CRFI) and SCF (SCFI), and no fertiliser. We found that treatments CRFIII, SCFIII, CRFII and SCFII produced grain yields that were 13.15%, 14.15%, 9.69% and 10.04% higher than CCF. There were no significant differences in grain yield among CRFI, SCFI and CCF. We also found that the ammonia volatilisation rates of CRF were significantly lower than those of CCF. The CRF treatments reduced the emission of ammonia by 51.34% to 91.34% compared to CCF. In addition, after treatment with CRF, maize exhibited a higher net photosynthetic rate than CCF after anthesis. Agronomic NUE and apparent N recovery were higher in the CRF treatment than in the CCF treatment. The N uptake and physiological NUE of the four yield-enhanced CRF treatments were higher than those of CCF. These results suggest that the increase in NUE in the CRF treatments was generally attributable to the higher photosynthetic rate and lower ammonia volatilisation compared to CCF-treated maize.", "keywords": ["0106 biological sciences", "2. Zero hunger", "Nitrogen", "Science", "Q", "R", "04 agricultural and veterinary sciences", "15. Life on land", "Zea mays", "01 natural sciences", "6. Clean water", "Ammonia", "Medicine", "Humans", "0401 agriculture", " forestry", " and fisheries", "Seasons", "Photosynthesis", "Volatilization", "Fertilizers", "Sulfur", "Research Article"], "contacts": [{"organization": "Peng Liu, Bin Zhao, Jiwang Zhang, Shuting Dong,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0070569"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0070569", "name": "item", "description": "10.1371/journal.pone.0070569", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0070569"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-08-02T00:00:00Z"}}, {"id": "10.1371/journal.pone.0111965", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:16Z", "type": "Journal Article", "created": "2015-06-08", "title": "The Effect Of Chemical Amendments Used For Phosphorus Abatement On Greenhouse Gas And Ammonia Emissions From Dairy Cattle Slurry: Synergies And Pollution", "description": "Land application of cattle slurry can result in incidental and chronic phosphorus (P) loss to waterbodies, leading to eutrophication. Chemical amendment of slurry has been proposed as a management practice, allowing slurry nutrients to remain available to plants whilst mitigating P losses in runoff. The effectiveness of amendments is well understood but their impacts on other loss pathways (so-called 'pollution swapping' potential) and therefore the feasibility of using such amendments has not been examined to date. The aim of this laboratory scale study was to determine how the chemical amendment of slurry affects losses of NH3, CH4, N2O, and CO2. Alum, FeCl2, Polyaluminium chloride (PAC)-and biochar reduced NH3 emissions by 92, 54, 65 and 77% compared to the slurry control, while lime increased emissions by 114%. Cumulative N2O emissions of cattle slurry increased when amended with alum and FeCl2 by 202% and 154% compared to the slurry only treatment. Lime, PAC and biochar resulted in a reduction of 44, 29 and 63% in cumulative N2O loss compared to the slurry only treatment. Addition of amendments to slurry did not significantly affect soil CO2 release during the study while CH4 emissions followed a similar trend for all of the amended slurries applied, with an initial increase in losses followed by a rapid decrease for the duration of the study. All of the amendments examined reduced the initial peak in CH4 emissions compared to the slurry only treatment. There was no significant effect of slurry amendments on global warming potential (GWP) caused by slurry land application, with the exception of biochar. After considering pollution swapping in conjunction with amendment effectiveness, the amendments recommended for further field study are PAC, alum and lime. This study has also shown that biochar has potential to reduce GHG losses arising from slurry application.", "keywords": ["Greenhouse Effect", "Time Factors", "Science", "methane emissions", "Nitrous Oxide", "n2o emissions", "Environment", "Global Warming", "soil", "12. Responsible consumption", "Ammonia", "Air Pollution", "Animals", "volatilization", "2. Zero hunger", "Air Pollutants", "Sewage", "Q", "Pollution swapping", "R", "Phosphorus", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Greenhouse Gas", "field", "6. Clean water", "livestock slurry", "Dairying", "Slurries", "13. Climate action", "manure", "nitrous-oxide emission", "Medicine", "Feasibility Studies", "0401 agriculture", " forestry", " and fisheries", "Cattle", "grassland", "Methane", "charcoal", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0111965"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0111965", "name": "item", "description": "10.1371/journal.pone.0111965", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0111965"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-06-08T00:00:00Z"}}, {"id": "10.1371/journal.pone.0092517", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:15Z", "type": "Journal Article", "created": "2014-04-02", "title": "Ammonium as a Driving Force of Plant Diversity and Ecosystem Functioning: Observations Based on 5 Years' Manipulation of N Dose and Form in a Mediterranean Ecosystem", "description": "Enhanced nitrogen (N) availability is one of the main drivers of biodiversity loss and degradation of ecosystem functions. However, in very nutrient-poor ecosystems, enhanced N input can, in the short-term, promote diversity. Mediterranean Basin ecosystems are nutrient-limited biodiversity hotspots, but no information is available on their medium- or long-term responses to enhanced N input. Since 2007, we have been manipulating the form and dose of available N in a Mediterranean Basin maquis in south-western Europe that has low ambient N deposition (<4 kg N ha(-1) yr(-1)) and low soil N content (0.1%). N availability was modified by the addition of 40 kg N ha(-1) yr(-1) as a 1\u22361 NH4Cl to (NH4)2SO4 mixture, and 40 and 80 kg N ha(-1) yr(-1) as NH4NO3. Over the following 5 years, the impacts on plant composition and diversity (richness and evenness) and some ecosystem characteristics (soil extractable N and organic matter, aboveground biomass and % of bare soil) were assessed. Plant species richness increased with enhanced N input and was more related to ammonium than to nitrate. Exposure to 40 kg NH4+-N ha(-1) yr(-1) (alone and with nitrate) enhanced plant richness, but did not increase aboveground biomass; soil extractable N even increased under 80 kg NH4NO3-N ha(-1) yr(-1) and the % of bare soil increased under 40 kg NH4+-N ha(-1) yr(-1). The treatment containing less ammonium, 40 kg NH4NO3-N ha(-1) yr(-1), did not enhance plant diversity but promoted aboveground biomass and reduced the % of bare soil. Data suggest that enhanced NHy availability affects the structure of the maquis, which may promote soil erosion and N leakage, whereas enhanced NOx availability leads to biomass accumulation which may increase the fire risk. These observations are relevant for land use management in biodiverse and fragmented ecosystems such as the maquis, especially in conservation areas.", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Mediterranean Region", "Science", "Q", "R", "Aquatic Ecology", "Biodiversity", "Plants", "15. Life on land", "6. Clean water", "03 medical and health sciences", "Ammonia", "13. Climate action", "Medicine", "Plant Physiological Phenomena", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0092517"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0092517", "name": "item", "description": "10.1371/journal.pone.0092517", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0092517"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-04-02T00:00:00Z"}}, {"id": "10.1371/journal.pone.0102315", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:16Z", "type": "Journal Article", "created": "2014-07-16", "title": "Soil Biochemical Responses To Nitrogen Addition In A Bamboo Forest", "description": "Many vital ecosystem processes take place in the soils and are greatly affected by the increasing active nitrogen (N) deposition observed globally. Nitrogen deposition generally affects ecosystem processes through the changes in soil biochemical properties such as soil nutrient availability, microbial properties and enzyme activities. In order to evaluate the soil biochemical responses to elevated atmospheric N deposition in bamboo forest ecosystems, a two-year field N addition experiment in a hybrid bamboo (Bambusa pervariabilis \u00d7 Dendrocalamopsis daii) plantation was conducted. Four levels of N treatment were applied: (1) control (CK, without N added), (2) low-nitrogen (LN, 50 kg N ha(-1) year(-1)), (3) medium-nitrogen (MN, 150 kg N ha(-1) year(-1)), and (4) high-nitrogen (HN, 300 kg N ha(-1) year(-1)). Results indicated that N addition significantly increased the concentrations of NH4(+), NO3(-), microbial biomass carbon, microbial biomass N, the rates of nitrification and denitrification; significantly decreased soil pH and the concentration of available phosphorus, and had no effect on the total organic carbon and total N concentration in the 0-20 cm soil depth. Nitrogen addition significantly stimulated activities of hydrolytic enzyme that acquiring N (urease) and phosphorus (acid phosphatase) and depressed the oxidative enzymes (phenol oxidase, peroxidase and catalase) activities. Results suggest that (1) this bamboo forest ecosystem is moving towards being limited by P or co-limited by P under elevated N deposition, (2) the expected progressive increases in N deposition may have a potential important effect on forest litter decomposition due to the interaction of inorganic N and oxidative enzyme activities, in such bamboo forests under high levels of ambient N deposition.", "keywords": ["Analysis of Variance", "China", "Atmosphere", "Monophenol Monooxygenase", "Nitrogen", "Science", "Q", "Acid Phosphatase", "R", "04 agricultural and veterinary sciences", "Forests", "15. Life on land", "Catalase", "Nitrification", "Soil", "Ammonia", "Denitrification", "Medicine", "Urea", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Sasa", "Soil Microbiology", "Research Article", "Peroxidase"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0102315"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0102315", "name": "item", "description": "10.1371/journal.pone.0102315", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0102315"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-07-16T00:00:00Z"}}, {"id": "10.1371/journal.pone.0115649", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:16Z", "type": "Journal Article", "created": "2015-01-30", "title": "In Situ Nitrogen Mineralization, Nitrification, And Ammonia Volatilization In Maize Field Fertilized With Urea In Huanghuaihai Region Of Northern China", "description": "Nitrogen (N) fertilization potentially affects soil N mineralization and leaching, and can enhance NH3 volatilization, thus impacting crop production. A fertilizer experiment with five levels of N addition (0, 79, 147, 215 and 375 kg N ha(-1)) was performed in 2009 and 2010 in a maize field in Huanghuaihai region, China, where > 300 kg N ha(-1) has been routinely applied to soil during maize growth period of 120 days. Responses of net N mineralization, inorganic N flux (0-10 cm), NH3 volatilization, and maize yield to N fertilization were measured. During the growth period, net N mineralization and nitrification varied seasonally, with higher rates occurring in August and coinciding with the R1 stage of maize growth. Soil NO3(-)-N contributed to more than 60% of inorganic N flux during maize growth. Cumulative NH3 volatilization increased with N additions, with total NH3 volatilization during maize growth accounting for about 4% of added N. Relative to the control, mean maize yield in the fertilizer treatments increased by 17% and 20% in 2009 and 2010, respectively. However, grain yield, aboveground biomass, and plant N accumulation did not increase with added N at levels > 215 kg N ha(-1). These results suggest that the current N rate of 300 kg N ha(-1) is not only excessive, but also reduces fertilizer efficacy and may contribute to environmental problems such as global warming and eutrophication of ground water and streams.", "keywords": ["2. Zero hunger", "China", "Nitrogen", "Science", "Q", "R", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Nitrification", "Zea mays", "01 natural sciences", "6. Clean water", "Soil", "Ammonia", "13. Climate action", "8. Economic growth", "Medicine", "Urea", "0401 agriculture", " forestry", " and fisheries", "Volatilization", "Fertilizers", "Research Article", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0115649"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0115649", "name": "item", "description": "10.1371/journal.pone.0115649", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0115649"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-01-30T00:00:00Z"}}, {"id": "10.1590/1413-70542017415003917", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:29Z", "type": "Journal Article", "created": "2017-11-15", "title": "Ammonia And Carbon Dioxide Emissions By Stabilized Conventional Nitrogen Fertilizers And Controlled Release In Corn Crop", "description": "ABSTRACT The market of stabilized, slow and controlled release nitrogen (N) fertilizers represents 1% of the world fertilizer consumption. On the other hand, the increase in availability, innovation and application of these technologies could lead to the improvement of N use efficiency in agroecossystems and to the reduction of environmental impacts. The objective of this study was to quantify agronomic efficiency relative index, ammonia volatilization, and CO2 emissions from conventional, stabilized and controlled release N fertilizers in corn summer crop. The experiment was carried out in a corn crop area located in Lavras, state of Minas Gerais, Brazil, without irrigation. All treatments were applied in topdressing at rate of 150 kg ha-1 N. N-NH3 losses from N fertilizers were: Granular urea (39% of the applied N ) = prilled urea (38%) > urea coated with 16% S0 (32%) = blend of urea + 7.9% S0 + polymers + conventional urea (32%) > prilled urea incorporated at 0.02 m depth (24%) > urea + 530 mg kg-1 of NBPT (8%) = Hydrolyzed leather (9%) > urea + thermoplastic resin (3%) = ammonium sulfate (1%) = ammonium nitrate (0.7%). Thermoplastic resin coated urea, ammonium nitrate and ammonium sulfate presented low values of cumulative CO2 emissions in corn crop. On the other hand, hydrolyzed leather promoted greater C-CO2 emission, when compared with other nitrogen fertilizers.
", "keywords": ["Coated urea", "Nitrogen", "Agriculture (General)", "Biomedical Engineering", "no-tillage", "Soil Science", "Organic chemistry", "Pesticide Pollution and Management", "Ammonia volatilization from urea", "FOS: Medical engineering", "Nitrate", "S1-972", "Agricultural and Biological Sciences", "Engineering", "Fertilizer", "Zea mays L.", "Ammonia", "perdas de NH3", "Agricultural Applications", "Urea", "Ammonium nitrate", "Ammonium sulfate", "Biology", "Effects of Soil Compaction on Crop Production", "4. Education", "Life Sciences", "04 agricultural and veterinary sciences", "Pollution", "Agronomy", "Chemistry", "plantio direto", "Controlled Release Materials for Agriculture", "Physical Sciences", "Environmental Science", "0401 agriculture", " forestry", " and fisheries", "NH3 loss", "CO2", "Ammonium"]}, "links": [{"href": "https://doi.org/10.1590/1413-70542017415003917"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ci%C3%AAncia%20e%20Agrotecnologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/1413-70542017415003917", "name": "item", "description": "10.1590/1413-70542017415003917", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/1413-70542017415003917"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-09-01T00:00:00Z"}}, {"id": "10.1590/s0100-06832014000400016", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:31Z", "type": "Journal Article", "created": "2014-10-04", "title": "Ammonia Volatilization And Yield Components After Application Of Polymer-Coated Urea To Maize", "description": "A form of increasing the efficiency of N fertilizer is by coating urea with polymers to reduce ammonia volatilization. The aim of this study was to evaluate the effect of polymer-coated urea on the control of ammonia volatilization, yield and nutritional characteristics of maize. The experiment was carried out during one maize growing cycle in 2009/10 on a Geric Ferralsol, inUberl\uffc3\uffa2ndia, MG, Brazil. Nitrogen fertilizers were applied as topdressing on the soil surface in the following urea treatments: polymer-coated urea at rates of 45, 67.5 and 90 kg ha-1 N and one control treatment (no N), in randomized blocks with four replications. Nitrogen application had a favorable effect on N concentrations in leaves and grains, Soil Plant Analysis Development (SPAD) chlorophyll meter readings and on grain yield, where as coated urea had no effect on the volatilization rates, SPAD readings and N leaf and grain concentration, nor on grain yield in comparison to conventional fertilization.
", "keywords": ["2. Zero hunger", "volatiliza\u00e7\u00e3o de am\u00f4nia", "Agriculture (General)", "N fertilizer", "polymer-coated urea", "leitura SPAD", "04 agricultural and veterinary sciences", "Zea mays", "nitrogen", "6. Clean water", "nitrog\u00eanio", "S1-972", "ammonia volatilization", "SPAD reading", "0401 agriculture", " forestry", " and fisheries", "fertilizante nitrogenado", "ureia revestida com pol\u00edmeros"]}, "links": [{"href": "https://doi.org/10.1590/s0100-06832014000400016"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Revista%20Brasileira%20de%20Ci%C3%AAncia%20do%20Solo", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s0100-06832014000400016", "name": "item", "description": "10.1590/s0100-06832014000400016", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s0100-06832014000400016"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-08-01T00:00:00Z"}}, {"id": "10.1590/s0100-204x2017000300007", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:32Z", "type": "Journal Article", "created": "2017-05-11", "title": "Additives Incorporated Into Urea To Reduce Nitrogen Losses After Application To The Soil", "description": "Abstract: The objective of this work was to develop urea-based fertilizers with internal incorporation of urease inhibitors and other additives in the granule. The effects of the incorporation of NBPT, copper (Cu+2), boric acid (H3BO3), elemental sulphur (S\uffc2\uffba), and a clay mineral from the zeolite group in powder urea - with ten different combinations of these additives - were evaluated as to N losses by volatilization and leaching. The losses in laboratory-developed formulations were compared with those of commercial fertilizers coated with the same additives (Super N, FH Nitro Mais, and FH Nitro Gold). The evaluations were made in greenhouse conditions, using a Ultisol accommodated in PVC columns. Nitrate and ammonium leaching was evaluated in the solution percolated through the soil columns. Ammonia volatilization was measured with a semi-open static chamber. The incorporation of urease inhibitors (NBPT, H3BO3, and Cu+2) into the urea granules was efficient to reduce N volatilization. Ammonia volatilization in the laboratory-developed ureas was lower than in commercial fertilizers coated with the same additives, while ammonium sulfate losses by leaching were similar. The addition of zeolite does not reduce N volatilization. Mineral N leaching in the soil profile is not affected by urease inhibitors.
", "keywords": ["volatiliza\u00e7\u00e3o de am\u00f4nia", "Agriculture (General)", "urease inhibitors", "04 agricultural and veterinary sciences", "lixivia\u00e7\u00e3o", "01 natural sciences", "inibidores de urease", "6. Clean water", "S1-972", "leaching", "nitrogen fertilizers", "ammonia volatilization", "NBPT", "0401 agriculture", " forestry", " and fisheries", "fertilizantes nitrogenados", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1590/s0100-204x2017000300007"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Pesquisa%20Agropecu%C3%A1ria%20Brasileira", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s0100-204x2017000300007", "name": "item", "description": "10.1590/s0100-204x2017000300007", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s0100-204x2017000300007"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-03-01T00:00:00Z"}}, {"id": "10.1590/s0103-90162008000400011", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:20:32Z", "type": "Journal Article", "created": "2010-01-08", "title": "Ammonia Volatilisation From Urease Inhibitor-Treated Urea Applied To Sugarcane Trash Blankets", "description": "Legal restrictions from burning sugarcane prior to harvest are causing a sharp increase in acreage which is harvested as green cane. The presence of a thick sugarcane trash mulch left after harvest makes it difficult to incorporate fertilisers in the soil. Since large losses of ammonia may occur when urea is surface applied to trash, it is important to find ways to improve urea-N use efficiency. The urease inhibitor NBPT slows down urea hydrolysis and thus may help decrease ammonia losses. Ammonia traps were set up in seven sugarcane fields covered with trash and fertilised with ammonium sulfate or ammonium nitrate, urea, and NBPT-treated urea. All N fertilisers were surface-applied at rates of 80 or 100 kg N ha-1. Very little N was lost when ammonium nitrate or ammonium sulfate were used. However, volatilisation losses as ammonia from the urea treatments varied from 1% (rainy days after fertilisation) to 25% of the applied N. The percentage of reduction in volatilisation due to NBPT application ranged from 15% to 78% depending on the weather conditions during the days following application of N. Addition of NBPT to urea helped to control ammonia losses, but the inhibitor was less effective when rain sufficient to incorporate urea into the soil occurred only 10 to 15 days or latter after fertiliser application.
", "keywords": ["adubos nitrogenados", "NBPT", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "perdas de am\u00f4nia", "ammonia losses", "nitrogen fertilizer"]}, "links": [{"href": "https://doi.org/10.1590/s0103-90162008000400011"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientia%20Agricola", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1590/s0103-90162008000400011", "name": "item", "description": "10.1590/s0103-90162008000400011", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1590/s0103-90162008000400011"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-01-01T00:00:00Z"}}, {"id": "10.1631/jzus.b1200013", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:34Z", "type": "Journal Article", "created": "2012-10-03", "title": "Abundance And Composition Of Ammonia-Oxidizing Bacteria And Archaea In Different Types Of Soil In The Yangtze River Estuary", "description": "Tidal flats are soil resources of great significance. Nitrification plays a central role in the nitrogen cycle and is often a critical first step in nitrogen removal from estuarine and coastal environments. We determined the abundance as well as composition of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) in different soils during land reclamation process. The abundance of AOA was higher than that of AOB in farm land and wild land while AOA was not detected in tidal flats using real-time polymerase chain reaction (PCR). The different abundances of AOB and AOA were negatively correlated with the salinity. The diversities of AOB and AOA were also investigated using clone libraries by amplification of amoA gene. Among AOB, nearly all sequences belonged to the Nitrosomonas lineage in the initial land reclamation process, i.e., tidal flats, while both Nitrosomonas and Nitrosospira lineages were detected in later and transition phases of land reclamation process, farm land and wild land. The ratio of the numbers of sequences of Nitrosomonas and Nitrosospira lineages was positively correlated with the salinity and the net nitrification rate. As for AOA, there was no obvious correlation with the changes in the physicochemical properties of the soil. This study suggests that AOB may be more import than AOA with respect to influencing the different land reclamation process stages.", "keywords": ["DNA", " Bacterial", "0301 basic medicine", "China", "Conservation of Natural Resources", "Archaeal Proteins", "Molecular Sequence Data", "Genes", " Archaeal", "03 medical and health sciences", "Bacterial Proteins", "Rivers", "Ammonia", "14. Life underwater", "Phylogeny", "0303 health sciences", "Bacteria", "Biodiversity", "Nitrogen Cycle", "15. Life on land", "Archaea", "Nitrification", "6. Clean water", "DNA", " Archaeal", "Genes", " Bacterial", "13. Climate action", "Estuaries", "Oxidoreductases", "Oxidation-Reduction"]}, "links": [{"href": "https://doi.org/10.1631/jzus.b1200013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Zhejiang%20University%20SCIENCE%20B", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1631/jzus.b1200013", "name": "item", "description": "10.1631/jzus.b1200013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1631/jzus.b1200013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-10-01T00:00:00Z"}}, {"id": "10.2134/jeq2005.0018", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:55Z", "type": "Journal Article", "created": "2005-08-10", "title": "Nitrogen Oxide And Methane Emissions Under Varying Tillage And Fertilizer Management", "description": "ABSTRACTComprehensive assessment of the total greenhouse gas (GHG) budget of reduced tillage agricultural systems must consider emissions of nitrous oxide (N2O) and methane (CH4), each of which have higher global warming potentials than carbon dioxide (CO2). Tillage intensity may also impact nitric oxide (NO) emissions, which can have various environmental and agronomic impacts. In 2003 and 2004, we used chambers to measure N2O, CH4, and NO fluxes from plots that had been managed under differing tillage intensity since 1991. The effect of tillage on non\uffe2\uff80\uff90CO2 GHG emissions varied, in both magnitude and direction, depending on fertilizer practices. Emissions of N2O following broadcast urea (BU) application were higher under no till (NT) and conservation tillage (CsT) compared to conventional tillage (CT). In contrast, following anhydrous ammonia (AA) injection, N2O emissions were higher under CT and CsT compared to NT. Emissions following surface urea ammonium nitrate (UAN) application did not vary with tillage. Total growing season non\uffe2\uff80\uff90CO2 GHG emissions were equivalent to CO2 emissions of 0.15 to 1.9 Mg CO2 ha\uffe2\uff88\uff921 yr\uffe2\uff88\uff921 or 0.04 to 0.53 Mg soil\uffe2\uff80\uff90C ha\uffe2\uff88\uff921 yr\uffe2\uff88\uff921 Emissions of N2O from AA\uffe2\uff80\uff90amended plots were two to four times greater than UAN\uffe2\uff80\uff90 and BU\uffe2\uff80\uff90amended plots. Total NO + N2O losses in the UAN treatment were approximately 50% lower than AA and BU. This study demonstrates that N2O emissions can represent a substantial component of the total GHG budget of reduced tillage systems, and that interactions between fertilizer and tillage practices can be important in controlling non\uffe2\uff80\uff90CO2 GHG emissions.
", "keywords": ["Greenhouse Effect", "2. Zero hunger", "Air Pollutants", "Analysis of Variance", "Chromatography", " Gas", "Time Factors", "Minnesota", "Nitrous Oxide", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Nitric Oxide", "7. Clean energy", "01 natural sciences", "6. Clean water", "Ammonia", "13. Climate action", "11. Sustainability", "Urea", "0401 agriculture", " forestry", " and fisheries", "Fertilizers", "Methane", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.2134/jeq2005.0018"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2005.0018", "name": "item", "description": "10.2134/jeq2005.0018", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2005.0018"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-09-01T00:00:00Z"}}, {"id": "10.2134/jeq2008.0295", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-06-23T16:20:56Z", "type": "Journal Article", "created": "2009-05-23", "title": "Banding Of Urea Increased Ammonia Volatilization In A Dry Acidic Soil", "description": "Volatilization of ammonia following application of urea contributes to smog formation and degradation of natural ecosystems. The objective of this study was to evaluate the impact of (i) incorporation and banding of urea and (ii) surface broadcast of slow\uffe2\uff80\uff90release urea types on NH3 volatilization in a dry acidic soil. Volatilization was measured using wind tunnels for 25 d after standard urea (140 kg N ha\uffe2\uff88\uff921) was broadcast, broadcast and incorporated (0\uffe2\uff80\uff935 cm), or incorporated in shallow bands (3\uffe2\uff80\uff935 cm) to a conventionally tilled silty loam soil. Urea supplemented with a urease inhibitor or coated with a polymer was also broadcast at the soil surface. Little N diffused out of the polymer\uffe2\uff80\uff90coated granules and ammonia losses were low (4% of applied N). Use of a urease inhibitor also resulted in a low NH3 loss (5% of applied N) while maintaining soil mineral N at levels similar to plots where untreated urea was broadcast. The rate of hydrolysis of urea broadcast at the soil surface was slowed by the lack of moisture and NH3 loss (9% applied N) was the lowest of all treatments with standard urea. Incorporation of broadcast urea increased emissions (16% applied N) by increasing urea hydrolysis relative to surface application. Furthermore, incorporation in band also increased emissions (27% applied N) due to a localized increase in soil pH from 6.0 to 8.7. We conclude that incorporating urea in bands in a dry acidic soil can increase NH3 volatilization compared to broadcast application followed by incorporation.
", "keywords": ["Soil", "Ammonia", "13. Climate action", "Urea", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "Volatilization", "Acids", "01 natural sciences", "6. Clean water", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Denis A. Angers, Marc-Olivier Gasser, J. Douglas MacDonald, Normand Bertrand, Philippe Rochette, Martin H. Chantigny,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.2134/jeq2008.0295"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2008.0295", "name": "item", "description": "10.2134/jeq2008.0295", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2008.0295"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-07-01T00:00:00Z"}}, {"id": "10.2134/jeq2008.0476", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:56Z", "type": "Journal Article", "created": "2009-08-25", "title": "Mineral-Nitrogen Leaching And Ammonia Volatilization From A Rice-Rapeseed System As Affected By 3,4-Dimethylpyrazole Phosphate", "description": "3,4\uffe2\uff80\uff90Dimethylpyrazole phosphate (DMPP) was validated as an effective nitrification inhibitor to reduce nitrate leaching. Its effects on ammonia (NH3) volatilization were not clear, especially on farmland scale with crop rotations. In this study, on\uffe2\uff80\uff90farm experiments at the Jiaxing (JX) and Yuhang (YH) sites in Taihu Lake Basin, China were conducted to evaluate the effect of DMPP application on mineral nitrogen (N) (NH4\uffe2\uff80\uff93N and NO3\uffe2\uff80\uff93N) leaching and NH3 volatilization losses in a rice\uffe2\uff80\uff93rapeseed cropping system. Treatments included urea alone (UA), urea + 1% DMPP (UD), and no fertilizer (CK). The results show that DMPP reduced NO3\uffe2\uff80\uff93N leaching fluxes by 44.9 to 59.9% and increased NH4\uffe2\uff80\uff93N leaching fluxes by 13.0 to 33.3% at two sites during rice and rape seasons compared with urea alone. Reductions in mineral\uffe2\uff80\uff90N leaching fluxes by DMPP in two seasons at the JX and YH sites were 9.5 and 14.3 kg N ha\uffe2\uff88\uff921, respectively, compared with UA treatment. The application of DMPP had no significant effects on NH3 volatilization loss fluxes at either site. The rice and rapeseed yields were 5.3 to 7.4% higher in UD plots than in UA plots at two sites. These results that indicate DMPP could reduce leaching losses of mineral\uffe2\uff80\uff90N from crop fields and promote grain yields by conserving more applied N in soil in rice\uffe2\uff80\uff93rapeseed rotation systems.
", "keywords": ["Crops", " Agricultural", "2. Zero hunger", "China", "Ammonia", "Nitrogen", "Brassica napus", "Pyrazoles", "0401 agriculture", " forestry", " and fisheries", "Oryza", "04 agricultural and veterinary sciences", "Volatilization", "Environmental Pollution", "6. Clean water"], "contacts": [{"organization": "Yanfeng Lian, Yingxu Chen, Wenhong Li, Hua Li, Xinqiang Liang,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.2134/jeq2008.0476"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2008.0476", "name": "item", "description": "10.2134/jeq2008.0476", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2008.0476"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-09-01T00:00:00Z"}}, {"id": "10.2134/jeq2011.0240", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:20:57Z", "type": "Journal Article", "created": "2011-12-05", "title": "Broadcast Urea Reduces N2o But Increases No Emissions Compared With Conventional And Shallow-Applied Anhydrous Ammonia In A Coarse-Textured Soil", "description": "Despite the importance of anhydrous ammonia (AA) and urea as nitrogen (N) fertilizer sources in the United States, there have been few direct comparisons of their effects on soil nitrous oxide (N2O) and nitric oxide (NO) emissions. We compared N oxide emissions, yields, and N fertilizer recovery efficiency (NFRE) in a corn (Zea mays L.) production system that used three different fertilizer practices: urea that was broadcast and incorporated (BU) and AA that was injected at a conventional depth (0.20 m) (AAc) and at a shallower depth (0.10 m) (AAs). Averaged over 2 yr in an irrigated loamy sand in Minnesota, growing season N2O emissions increased in the order BU < AAc < AAs. In contrast, NO emissions were greater with BU than with AAc or AAs. Emissions of N2O ranged from 0.5 to 1.4 kg N ha\uffe2\uff88\uff921 (50\uffe2\uff80\uff93140 g N Mg\uffe2\uff88\uff921 grain), while NO emissions ranged from 0.2 to 0.7 kg N ha\uffe2\uff88\uff921 (20\uffe2\uff80\uff9370 g N Mg\uffe2\uff88\uff921 grain). Emissions of total N oxides (NO + N2O) increased in the order AAc < BU < AAs. Despite having the greatest emissions of N2O and total N oxides, the AAs treatment had greater NFRE compared with the AAc treatment. These results provide additional evidence that AA emits more N2O, but less NO, than broadcast urea and show that practices to reduce N2O emissions do not always improve N use efficiency.
", "keywords": ["2. Zero hunger", "Environmental Engineering", "Monitoring", "Policy and Law", "Climate", "Nitrous Oxide", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Nitric Oxide", "Pollution", "Zea mays", "01 natural sciences", "6. Clean water", "Management", "Soil", "Ammonia", "13. Climate action", "Urea", "0401 agriculture", " forestry", " and fisheries", "Waste Management and Disposal", "Water Science and Technology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.2134/jeq2011.0240"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2011.0240", "name": "item", "description": "10.2134/jeq2011.0240", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2011.0240"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-11-01T00:00:00Z"}}, {"id": "10.4067/s0718-58392015000500015", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:22:04Z", "type": "Journal Article", "created": "2015-10-09", "title": "Effect Of Chemical Fertilization And Green Manure On The Abundance And Community Structure Of Ammonia Oxidizers In A Paddy Soil", "description": "Ammonia oxidization is a critical step in the soil N cycle and can be affected by the fertilization regimes. Chinese milk-vetch (Astragalus sinicus L., MV) is a major green manure of rice (Oryza sativa L.) fields in southern China, which is recommended as an important agronomic practice to improve soil fertility. Soil chemical properties, abundance and community structures of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) in a MV-rice rotation field under different fertilization regimes were investigated. The field experiment included six treatments: control, without MV and chemical fertilizer (CK); 100% chemical fertilizer (NPK); 18 000 kg MV ha-1 plus 100% chemical fertilizer (NPKM1); 18 000 kg MV ha-1 plus 40% chemical fertilizer (NPKM2); 18 000 kg MV ha-1 alone (MV); and 18 000 kg MV ha-1 plus 40% chemical fertilizer plus straw (NPKMS). Results showed that NPKMS treatment could improve the soil fertility greatly although the application of 60% chemical fertilizer. The abundance of AOB only in the MV treatment had significant difference with the control; AOA were more abundant than AOB in all corresponding treatments. The NPKMS treatment had the highest AOA abundance (1.19 x 108 amoA gene copies g-1) and the lowest abundance was recorded in the CK treatment (3.21 x 107 amoA gene copies g-1). The abundance of AOA was significantly positively related to total N, available N, NH4+-N, and NO3--N. The community structure of AOA exhibited little variation among different fertilization regimes, whereas the community structure of AOB was highly responsive. Phylogenetic analysis showed that all AOB sequences were affiliated with Nitrosospira or Nitrosomonas and all AOA denaturing gradient gel electrophoresis (DGGE) bands belonged to the soil and sediment lineage. These findings could be fundamental to improve our understanding of AOB and AOA in the N cycle in the paddy soil.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "03 medical and health sciences", "Astragalus sinicus", "soil chemical properties", "Abundance", "ammonia-oxidizing bacteria (AOB)", "Chinese milk vetch", "community structure", "15. Life on land", "ammonia-oxidizing archaea (AOA)", "6. Clean water"], "contacts": [{"organization": "Fang, Yu, Yan, Zhi-Lei, Chen, Ji-Chen, Wang, Fei, Wang, Ming-Kuang, Lin, Xin-Jian,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.4067/s0718-58392015000500015"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chilean%20journal%20of%20agricultural%20research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.4067/s0718-58392015000500015", "name": "item", "description": "10.4067/s0718-58392015000500015", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.4067/s0718-58392015000500015"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-12-01T00:00:00Z"}}, {"id": "10.2527/2005.831182x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:21:22Z", "type": "Journal Article", "created": "2016-08-09", "description": "The objective of this study was to compare methane emission by goats consuming the condensed tannin-containing forage sericea lespedeza (Les-pedeza cuneata) or a mixture of crabgrass (Digitaria ischaemum) and Kentucky 31 tall fescue (Festuca arundinacea). Two groups of 12 Angora does (initial average BW = 41.5 +/- 2.7 kg) that previously grazed a pasture of sericea lespedeza or crabgrass/tall fescue for approximately 4 mo were used. After 1 wk of adaptation to metabolism cages, gas exchange was measured for 24 h in an open-circuit respiration calorimetry system with four head boxes. Forage harvested daily from the previously grazed pastures was consumed ad libitum. Crude protein concentration was 10.3 and 13.0%, IVDMD was 64.5 and 75.3%, and the level of condensed tannins was 17.7 and 0.5% for sericea lespedeza and crabgrass/tall fescue, respectively. Dry matter intake (1.11 vs. 0.67 kg/d) and digestible DMI (estimated from IVDMD; 0.71 vs. 0.51 kg/d) were greater (P < 0.01) for sericea lespedeza than for crabgrass/tall fescue. Ruminal ammonia N (3.7 and 9.9 mg/dL; P < 0.001) and plasma urea-N concentrations (16.7 and 20.9 mg/dL; P = 0.07) were lower for sericea lespedeza than for crabgrass/tall fescue. Concentrations of individual and total VFA and the acetate-to-propionate ratio in ruminal fluid did not differ between treatments (P > 0.19). Despite higher DMI by goats fed sericea lespedeza, daily energy expenditure (409 vs. 431 kJ/kg BW(0.75)), heart rate (70 vs. 73 beats/min), and the ratio of energy expenditure to heart rate (5.82 vs. 5.94) did not differ between sericea lespedeza and crabgrass/tall fescue, respectively (P > 0.13). Methane emission expressed as both quantity per day or relative to DMI was lower (P <0.001) for sericea lespedeza than for crabgrass/tall fescue (7.4 vs. 10.6 g/d and 6.9 vs. 16.2 g/kg DMI). Substantial differences between the forages in condensed tannins concentration and methane emission by Angora goats suggest that condensed tannins decreased methane emission.", "keywords": ["Festuca", "2. Zero hunger", "Rumen", "Pulmonary Gas Exchange", "Goats", "Body Weight", "0402 animal and dairy science", "Lespedeza", "04 agricultural and veterinary sciences", "Fatty Acids", " Volatile", "Animal Feed", "Blood Urea Nitrogen", "Eating", "Ammonia", "13. Climate action", "Digitaria", "Animals", "Animal Nutritional Physiological Phenomena", "Female", "Proanthocyanidins", "Methane"]}, "links": [{"href": "https://doi.org/10.2527/2005.831182x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Animal%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2527/2005.831182x", "name": "item", "description": "10.2527/2005.831182x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2527/2005.831182x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-01-01T00:00:00Z"}}, {"id": "10.2527/jas.2009-1786", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:21:23Z", "type": "Journal Article", "created": "2009-06-20", "title": "Effect Of Sward Dry Matter Digestibility On Methane Production, Ruminal Fermentation, And Microbial Populations Of Zero-Grazed Beef Cattle", "description": "Increasing the digestibility of pasture for grazing ruminants has been proposed as a low-cost practical means of reducing ruminant CH(4) emissions. At high feed intake levels, the proportion of energy lost as CH(4) decreases as the digestibility of the diet increases. Therefore, improving forage digestibility may improve productivity as DM and energy intake are increased. A zero-grazing experiment was conducted to determine the effect of sward DM digestibility (DMD) on DMI, CH(4) emissions, and indices of rumen fermentation of beef animals. Twelve Charolais-cross heifers were assigned to 1 of 2 treatments, with 6 heifers per dietary treatment. Additionally, 4 cannulated Aberdeen Angus-cross steers were randomly allocated to each of these 2 treatments in a crossover design. Dietary treatments consisted of swards managed to produce (i) high digestibility pasture (high DMD) or (ii) pasture with less digestibility (low DMD), both offered for ad libitum intake. All animals were zero-grazed and offered freshly cut herbage twice daily. In vitro DMD values for the high and low DMD swards were 816 and 706 g/kg of DM. Heifers offered the high DMD grass had greater (P < 0.001) daily DMI of 7.66 kg compared with 5.38 kg for those offered the low DMD grass. Heifers offered the high DMD grass had greater (P = 0.003) daily CH(4) production (193 g of CH(4)/d) than those offered the low DMD grass (138 g of CH(4)/d). However, when corrected for DMI, digestible DMI, or ingested gross energy, there was no difference (P > 0.05) in CH(4) production between dietary treatments. For cannulated steers, intake tended (P = 0.06) to be greater for the high DMD grass (5.56 vs. 4.27 kg of DM/d), but rumen protozoa (4.95 x 10(4)/mL; P = 0.62); rumen ammonia (34 mg of N/L; P = 0.24); rumen total VFA (103 mM; P = 0.58), and rumen pH (6.8; P = 0.43) did not differ between treatments. There was no difference in total bacteria numbers, relative expression of the mcrA gene, and numbers of cycles to threshold for fungi when determined using quantitative PCR between dietary treatments with mean values of 73.0 ng/microL, 0.958, and 21.75 C(T), respectively. Results of this study demonstrate that there was no difference in CH(4) production when corrected for intake or rumen fermentation variables of beef cattle offered a high or low digestibility sward.", "keywords": ["DNA", " Bacterial", "Male", "2. Zero hunger", "Rumen", "0402 animal and dairy science", "04 agricultural and veterinary sciences", "Fatty Acids", " Volatile", "Polymerase Chain Reaction", "Random Allocation", "Ammonia", "RNA", " Ribosomal", " 16S", "Lolium", "Animals", "Cattle", "Digestion", "Female", "Least-Squares Analysis", "Methane"]}, "links": [{"href": "https://doi.org/10.2527/jas.2009-1786"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Animal%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2527/jas.2009-1786", "name": "item", "description": "10.2527/jas.2009-1786", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2527/jas.2009-1786"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-06-19T00:00:00Z"}}, {"id": "10.2527/jas.2011-4209", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:21:23Z", "type": "Journal Article", "created": "2012-01-28", "title": "Dietary Nitrate Supplementation Reduces Methane Emission In Beef Cattle Fed Sugarcane-Based Diets", "description": "The objective of this study was to determine the effect of dietary nitrate on methane emission and rumen fermentation parameters in Nellore \u00d7 Guzera (Bos indicus) beef cattle fed a sugarcane based diet. The experiment was conducted with 16 steers weighing 283 \u00b1 49 kg (mean \u00b1 SD), 6 rumen cannulated and 10 intact steers, in a cross-over design. The animals were blocked according to BW and presence or absence of rumen cannula and randomly allocated to either the nitrate diet (22 g nitrate/kg DM) or the control diet made isonitrogenous by the addition of urea. The diets consisted of freshly chopped sugarcane and concentrate (60:40 on DM basis), fed as a mixed ration. A 16-d adaptation period was used to allow the rumen microbes to adapt to dietary nitrate. Methane emission was measured using the sulfur hexafluoride tracer technique. Dry matter intake (P = 0.09) tended to be less when nitrate was present in the diet compared with the control, 6.60 and 7.05 kg/d DMI, respectively. The daily methane production was reduced (P < 0.01) by 32% when steers were fed the nitrate diet (85 g/d) compared with the urea diet (125 g/d). Methane emission per kilogram DMI was 27% less (P < 0.01) on the nitrate diet (13.3 g methane/kg DMI) than on the control diet (18.2 g methane/kg DMI). Methane losses as a fraction of gross energy intake (GEI) were less (P < 0.01) on the nitrate diet (4.2% of GEI) than on the control diet (5.9% of GEI). Nitrate mitigated enteric methane production by 87% of the theoretical potential. The rumen fluid ammonia-nitrogen (NH(3)-N()) concentration was significantly greater (P < 0.05) for the nitrate diet. The total concentration of VFA was not affected (P = 0.61) by nitrate in the diet, while the proportion of acetic acid tended to be greater (P = 0.09), propionic acid less (P = 0.06) and acetate/propionate ratio tended to be greater (P = 0.06) for the nitrate diet. Dietary nitrate reduced enteric methane emission in beef cattle fed sugarcane based diet.", "keywords": ["sheep", "Rumen", "Sulfur Hexafluoride", "urea", "mitigation", "Ammonia", "Animals", "Urea", "wall", "nitrite", "2. Zero hunger", "Cross-Over Studies", "Nitrates", "0402 animal and dairy science", "methanogenesis", "04 agricultural and veterinary sciences", "Animal Feed", "Diet", "Saccharum", "livestock", "rumen fermentation", "13. Climate action", "manipulation", "Dietary Supplements", "dairy", "Animal Nutritional Physiological Phenomena", "Cattle", "Methane"]}, "links": [{"href": "https://doi.org/10.2527/jas.2011-4209"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Animal%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2527/jas.2011-4209", "name": "item", "description": "10.2527/jas.2011-4209", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2527/jas.2011-4209"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-07-01T00:00:00Z"}}, {"id": "10.2527/jas1986.6362044x", "type": "Feature", "geometry": null, "properties": {"license": "unspecified", "updated": "2026-06-23T16:21:23Z", "type": "Journal Article", "created": "2016-08-09", "description": "Eight steers (327 kg average) were fed diets containing either nontreated or anhydrous NH3-treated wheat straw in a two-period crossover design. Intake and digestibilities of dry matter, gross energy, neutral detergent fiber, acid detergent fiber and crude protein were measured by total collection. Energy losses in urine and methane were determined. Heat production was determined by indirect respiration calorimetry on steers fed both diets and after they were fasted. Ammoniation of the straw raised its N content from .49 to 1.59% (P less than .001). Dry matter (DM) intake was increased (P less than .01) by the process from 1.0 to 1.3% of body weight. Digestibility coefficients of dry matter and energy, as well as those for fiber, were increased (P less than .001) by four percentage units or more. Crude protein digestibility, however, was depressed (P less than .001) from 67.8% to 53.5% by ammoniation. Urinary energy as a percentage of gross energy intake (GEI) was reduced (P less than .05) by NH3 treatment from 4.10 to 3.74%. Methane energy was not different (P greater than .10). Metabolizable energy was improved (P less than .001) by ammoniation, increasing from 45.2 to 50.0% of GEI. Daily heat production was higher (P less than .01) for steers consuming the ammoniated straw diet, increasing from 113.7 to 125.3 kcal/wt.75), and was due to higher metabolizable energy intake (MEI) since partial efficiency of MEI used for maintenance did not differ (P greater than .10) between diets. The higher net energy value of the ammoniated wheat straw diet (1.45 vs 1.26 kcal/g DM) was due mainly to decreased fecal loss and a slight decline in urinary loss.", "keywords": ["Male", "2. Zero hunger", "Ammonia", "0402 animal and dairy science", "Animals", "Cattle", "04 agricultural and veterinary sciences", "Energy Metabolism", "Animal Feed", "Nutritive Value", "6. Clean water"], "contacts": [{"organization": "C. P. Birkelo, D. E. Johnson, Gerald M. Ward,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.2527/jas1986.6362044x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Animal%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2527/jas1986.6362044x", "name": "item", "description": "10.2527/jas1986.6362044x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2527/jas1986.6362044x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1986-12-01T00:00:00Z"}}, {"id": "10.3389/fpls.2017.02260", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:21:33Z", "type": "Journal Article", "created": "2018-01-22", "description": "Agroecosystem nitrogen (N) loss produces greenhouse gases, induces eutrophication, and is costly for farmers; therefore, conservation agricultural management practices aimed at reducing N loss are increasingly adopted. However, the ecosystem consequences of these practices have not been well-studied. We quantified N loss via leaching, NH3 volatilization, N2O emissions, and N retention in plant and soil pools of corn conservation agroecosystems in Kentucky, USA. Three systems were evaluated: (1) an unfertilized, organic system with cover crops hairy vetch (Vicia villosa), winter wheat (Triticum aestivum), or a mix of the two (bi-culture); (2) an organic system with a hairy vetch cover crop employing three fertilization schemes (0 N, organic N, or a fertilizer N-credit approach); and (3) a conventional system with a winter wheat cover crop and three fertilization schemes (0 N, urea N, or organic N). In the unfertilized organic system, cover crop species affected NO3-N leaching (vetch > bi-culture > wheat) and N2O-N emissions and yield during corn growth (vetch, bi-culture > wheat). Fertilization increased soil inorganic N, gaseous N loss, N leaching, and yield in the organic vetch and conventional wheat systems. Fertilizer scheme affected the magnitude of growing season N2O-N loss in the organic vetch system (organic N > fertilizer N-credit) and the timing of loss (organic N delayed N2O-N loss vs. urea) and NO3-N leaching (urea >> organic N) in the conventional wheat system, but had no effect on yield. Cover crop selection and N fertilization techniques can reduce N leaching and greenhouse gas emissions without sacrificing yield, thereby enhancing N conservation in both organic and conventional conservation agriculture systems.", "keywords": ["2. Zero hunger", "nitrous oxide emissions", "Plant culture", "Plant Science", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "SB1-1110", "conservation agriculture", "ammonia volatilization", "13. Climate action", "nitrate leaching", "0401 agriculture", " forestry", " and fisheries", "cover crops"]}, "links": [{"href": "https://doi.org/10.3389/fpls.2017.02260"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2017.02260", "name": "item", "description": "10.3389/fpls.2017.02260", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2017.02260"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-01-22T00:00:00Z"}}, {"id": "10.3390/cells9092026", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:21:40Z", "type": "Journal Article", "created": "2020-09-03", "title": "Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress", "description": "Alterations of hydrogen peroxide (H2O2) levels have a profound impact on numerous signaling cascades orchestrating plant growth, development, and stress signaling, including programmed cell death. To expand the repertoire of known molecular mechanisms implicated in H2O2 signaling, we performed a forward chemical screen to identify small molecules that could alleviate the photorespiratory-induced cell death phenotype of Arabidopsisthaliana mutants lacking H2O2-scavenging capacity by peroxisomal catalase2. Here, we report the characterization of pakerine, an m-sulfamoyl benzamide from the sulfonamide family. Pakerine alleviates the cell death phenotype of cat2 mutants exposed to photorespiration-promoting conditions and delays dark-induced senescence in wild-type Arabidopsis leaves. By using a combination of transcriptomics, metabolomics, and affinity purification, we identified abnormal inflorescence meristem 1 (AIM1) as a putative protein target of pakerine. AIM1 is a 3-hydroxyacyl-CoA dehydrogenase involved in fatty acid \u03b2-oxidation that contributes to jasmonic acid (JA) and salicylic acid (SA) biosynthesis. Whereas intact JA biosynthesis was not required for pakerine bioactivity, our results point toward a role for \u03b2-oxidation-dependent SA production in the execution of H2O2-mediated cell death.
To follow the hypothesis that agricultural management practices affect structure and function of the soil microbiome regarding soil health and plant-beneficial traits, high-throughput (HT) metagenome analyses were performed on Chernozem soil samples from a long-term field experiment designated LTE-1 carried out at Bernburg-Strenzfeld (Saxony-Anhalt, Germany). Metagenomic DNA was extracted from soil samples representing the following treatments: (i) plough tillage with standard nitrogen fertilization and use of fungicides and growth regulators, (ii) plough tillage with reduced nitrogen fertilization (50%), (iii) cultivator tillage with standard nitrogen fertilization and use of fungicides and growth regulators, and (iv) cultivator tillage with reduced nitrogen fertilization (50%). Bulk soil (BS), as well as root-affected soil (RS), were considered for all treatments in replicates. HT-sequencing of metagenomic DNA yielded approx. 100 Giga bases (Gb) of sequence information. Taxonomic profiling of soil communities revealed the presence of 70 phyla, whereby Proteobacteria, Actinobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, Thaumarchaeota, Firmicutes, Verrucomicrobia and Chloroflexi feature abundances of more than 1%. Functional microbiome profiling uncovered, i.a., numerous potential plant-beneficial, plant-growth-promoting and biocontrol traits predicted to be involved in nutrient provision, phytohormone synthesis, antagonism against pathogens and signal molecule synthesis relevant in microbe\uffe2\uff80\uff93plant interaction. Neither taxonomic nor functional microbiome profiling based on single-read analyses revealed pronounced differences regarding the farming practices applied. Soil metagenome sequences were assembled and taxonomically binned. The ten most reliable and abundant Metagenomically Assembled Genomes (MAGs) were taxonomically classified and metabolically reconstructed. Importance of the phylum Thaumarchaeota for the analyzed microbiome is corroborated by the fact that the four corresponding MAGs were predicted to oxidize ammonia (nitrification), thus contributing to the cycling of nitrogen, and in addition are most probably able to fix carbon dioxide. Moreover, Thaumarchaeota and several bacterial MAGs also possess genes with predicted functions in plant\uffe2\uff80\uff93growth\uffe2\uff80\uff93promotion. Abundances of certain MAGs (species resolution level) responded to the tillage practice, whereas the factors compartment (BS vs. RS) and nitrogen fertilization only marginally shaped MAG abundance profiles. Hence, soil management regimes promoting plant-beneficial microbiome members are very likely advantageous for the respective agrosystem, its health and carbon sequestration and accordingly may enhance plant productivity. Since Chernozem soils are highly fertile, corresponding microbiome data represent a valuable reference resource for agronomy in general.
", "keywords": ["0301 basic medicine", "570", "plant\u2013growth\u2013promotion (PGP)", "metagenomically-assembled-genomes (MAGs)", "Article", "03 medical and health sciences", "carbon dioxide fixation", "Ammonia", "metagenomic binning", "Germany", "soil microbiome", "Proteobacteria", "Humans", "biocontrol", "secondary metabolite synthesis", "suppressive soil", "Phylogeny", "Soil Microbiology", "soil microbiome; suppressive soil; biocontrol; plant\u2013growth\u2013promotion (PGP); metagenomic binning; metagenomically-assembled-genomes (MAGs); secondary metabolite synthesis; carbon dioxide fixation; carbohydrate-active enzymes; differentially abundant features (DAFs)", "2. Zero hunger", "Bacteria", "Bacteroidetes", "Agriculture", "differentially abundant features (DAFs)", "15. Life on land", "Archaea", "Actinobacteria", "carbohydrate-active enzymes", "Metagenome"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/10/6/424/pdf"}, {"href": "https://www.mdpi.com/2073-4425/10/6/424/pdf"}, {"href": "https://doi.org/10.3390/genes10060424"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/genes10060424", "name": "item", "description": "10.3390/genes10060424", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/genes10060424"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-03T00:00:00Z"}}, {"id": "10.3390/ijerph8051491", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:21:44Z", "type": "Journal Article", "created": "2011-05-11", "description": "Application of poultry litter (PL) to soil may lead to nitrogen (N) losses through ammonia (NH3) volatilization and to potential contamination of surface runoff with PL-derived phosphorus (P). Amending litter with acidified biochar may minimize these problems by decreasing litter pH and by retaining litter-derived P, respectively. This study evaluated the effect of acidified biochars from pine chips (PC) and peanut hulls (PH) on NH3 losses and inorganic N and P released from surface-applied or incorporated PL. Poultry litter with or without acidified biochars was surface-applied or incorporated into the soil and incubated for 21 d. Volatilized NH3 was determined by trapping it in acid. Inorganic N and P were determined by leaching the soil with 0.01 M of CaCl2 during the study and by extracting it with 1 M KCl after incubation. Acidified biochars reduced NH3 losses by 58 to 63% with surface-applied PL, and by 56 to 60% with incorporated PL. Except for PH biochar, which caused a small increase in leached NH4+-N with incorporated PL, acidified biochars had no effect on leached or KCl-extractable inorganic N and P from surface-applied or incorporated PL. These results suggest that acidified biochars may decrease NH3 losses from PL but may not reduce the potential for P loss in surface runoff from soils receiving PL.
", "keywords": ["2. Zero hunger", "Water Pollution", "04 agricultural and veterinary sciences", "Phosphorus Compounds", "15. Life on land", "Article", "Poultry", "6. Clean water", "Manure", "acidified biochar; poultry litter; inorganic nitrogen; inorganic phosphorus; ammonia volatilization", "Ammonia", "13. Climate action", "Charcoal", "Animals", "0401 agriculture", " forestry", " and fisheries", "Volatilization", "Nitrogen Compounds"], "contacts": [{"organization": "William P. Miller, Sarah A. Doydora, Keshav C. Das, Julia W. Gaskin, Leticia Sonon, Miguel L. Cabrera,", "roles": ["creator"]}]}, "links": [{"href": "http://www.mdpi.com/1660-4601/8/5/1491/pdf"}, {"href": "https://doi.org/10.3390/ijerph8051491"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Environmental%20Research%20and%20Public%20Health", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/ijerph8051491", "name": "item", "description": "10.3390/ijerph8051491", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/ijerph8051491"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-05-11T00:00:00Z"}}, {"id": "10.3390/ijms15069907", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:21:44Z", "type": "Journal Article", "created": "2014-06-03", "title": "The Influence Of Ecological And Conventional Plant Production Systems On Soil Microbial Quality Under Hops (Humulus Lupulus)", "description": "The knowledge about microorganisms\uffe2\uff80\uff94activity and diversity under hop production is still limited. We assumed that, different systems of hop production (within the same soil and climatic conditions) significantly influence on the composition of soil microbial populations and its functional activity (metabolic potential). Therefore, we compared a set of soil microbial properties in the field experiment of two hop production systems (a) ecological based on the use of probiotic preparations and organic fertilization (b) conventional\uffe2\uff80\uff94with the use of chemical pesticides and mineral fertilizers. Soil analyses included following microbial properties: The total number microorganisms, a bunch of soil enzyme activities, the catabolic potential was also assessed following Biolog EcoPlates\uffc2\uffae. Moreover, the abundance of ammonia-oxidizing archaea (AOA) was characterized by terminal restriction fragment length polymorphism analysis (T-RFLP) of PCR ammonia monooxygenase \uffce\uffb1-subunit (amoA) gene products. Conventional and ecological systems of hop production were able to affect soil microbial state in different seasonal manner. Favorable effect on soil microbial activity met under ecological, was more probably due to livestock-based manure and fermented plant extracts application. No negative influence on conventional hopyard soil was revealed. Both type of production fulfilled fertilizing demands. Under ecological production it was due to livestock-based manure fertilizers and fermented plant extracts application.
", "keywords": ["2. Zero hunger", "Organic Agriculture", "9. Industry and infrastructure", "04 agricultural and veterinary sciences", "15. Life on land", "Archaea", "Article", "3. Good health", "Soil", "Ammonia", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "diversity; organic farming; soil microbial functionality", "Fertilizers", "Humulus", "Soil Microbiology"]}, "links": [{"href": "http://www.mdpi.com/1422-0067/15/6/9907/pdf"}, {"href": "https://doi.org/10.3390/ijms15069907"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Molecular%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/ijms15069907", "name": "item", "description": "10.3390/ijms15069907", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/ijms15069907"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-06-03T00:00:00Z"}}, {"id": "10.3390/su13031436", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:21:54Z", "type": "Journal Article", "created": "2021-01-29", "title": "Horticulture and Orchards as New Markets for Manure Valorisation with Less Environmental Impacts", "description": "Animal manure management is a real challenge to minimize environmental impacts and ensure that this valuable material is efficiently used in a circular economy context. One of the main limitations for larger use of animal manure as fertilizer is the availability of land to receive it in an area close to the farm. Indeed, animal manure is traditionally used for cereals and animal feed growth, but the soil area occupied with these crops might not be enough to receive all the manure produced and/or part of this soil might have nutrient contents, namely phosphorous, that do not permit further application of manure. Hence, extra land used for other agricultural activities might be an option. The main objective of the present review was to analyse the constraints and solutions to increase the use of manure in horticulture and orchards. Emphasis was given to the legal framework for manure utilization in the EU that might stimulate or restrain such a solution. The main characteristics of manure that might limit or stimulate manure reuse were also described, and the potential of some treatments to valorise manure was analysed. Several examples of alternative uses of manure in horticulture and orchards were examined, and the society and farmers\uffe2\uff80\uff99 acceptance of the proposed solution was addressed.
", "keywords": ["2. Zero hunger", "farmer's acceptance", "pathogens", "water contamination", "04 agricultural and veterinary sciences", "15. Life on land", "ammonia", "01 natural sciences", "12. Responsible consumption", "organic fertilizer", "13. Climate action", "greenhouse gases", "0401 agriculture", " forestry", " and fisheries", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.mdpi.com/2071-1050/13/3/1436/pdf"}, {"href": "https://doi.org/10.3390/su13031436"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sustainability", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/su13031436", "name": "item", "description": "10.3390/su13031436", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/su13031436"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-29T00:00:00Z"}}, {"id": "10.5194/essd-13-367-2021", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-23T16:22:36Z", "type": "Journal Article", "created": "2021-02-13", "title": "Copernicus Atmosphere Monitoring Service TEMPOral profiles (CAMS-TEMPO): global and European emission temporal profile maps for atmospheric chemistry modelling", "description": "Abstract. We present the Copernicus Atmosphere Monitoring Service TEMPOral profiles (CAMS-TEMPO), a dataset of global and European emission temporal profiles that provides gridded monthly, daily, weekly and hourly weight factors for atmospheric chemistry modelling. CAMS-TEMPO includes temporal profiles for the priority air pollutants (NOx; SOx; NMVOC, non-methane volatile organic compound; NH3; CO; PM10; and PM2.5) and the greenhouse gases (CO2 and CH4) for each of the following anthropogenic source categories: energy industry (power plants), residential combustion, manufacturing industry, transport (road traffic and air traffic in airports) and agricultural activities (fertilizer use and livestock). The profiles are computed on a global 0.1\uffe2\uff80\uff89\uffc3\uff97\uffe2\uff80\uff890.1\uffe2\uff88\uff98 and regional European 0.1\uffe2\uff80\uff89\uffc3\uff97\uffe2\uff80\uff890.05\uffe2\uff88\uff98 grid following the domain and sector classification descriptions of the global and regional emission inventories developed under the CAMS programme. The profiles account for the variability of the main emission drivers of each sector. Statistical information linked to emission variability (e.g. electricity production and traffic counts) at national and local levels were collected and combined with existing meteorology-dependent parametrizations to account for the influences of sociodemographic factors and climatological conditions. Depending on the sector and the temporal resolution (i.e. monthly, weekly, daily and hourly) the resulting profiles are pollutant-dependent, year-dependent (i.e. time series from 2010 to 2017) and/or spatially dependent (i.e. the temporal weights vary per country or region). We provide a complete description of the data and methods used to build the CAMS-TEMPO profiles, and whenever possible, we evaluate the representativeness of the proxies used to compute the temporal weights against existing observational data. We find important discrepancies when comparing the obtained temporal weights with other currently used datasets. The CAMS-TEMPO data product including the global (CAMS-GLOB-TEMPOv2.1, https://doi.org/10.24380/ks45-9147, Guevara et al., 2020a) and regional European (CAMS-REG-TEMPOv2.1, https://doi.org/10.24380/1cx4-zy68, Guevara et al., 2020b) temporal profiles are distributed from the Emissions of atmospheric Compounds and Compilation of Ancillary Data (ECCAD) system (https://eccad.aeris-data.fr/, last access: February 2021).
", "keywords": ["China", "Atmospheric chemistry", "550", "Anthropogenic emissions", "Ammonia emissions", "Urbanisation", "Environment", "7. Clean energy", "[SDU] Sciences of the Universe [physics]", "11. Sustainability", "Air-pollution", "GE1-350", "Gridded emissions", "Fuel use", "QE1-996.5", "[SDU.OCEAN] Sciences of the Universe [physics]/Ocean", " Atmosphere", "Inventory", "Geology", "Environmental sciences", "Data product", "Qu\u00edmica atmosf\u00e8rica", "13. Climate action", "Air quality", "Transport model", "Data sets", "Bottom-up", "\u00c0rees tem\u00e0tiques de la UPC::Desenvolupament hum\u00e0 i sostenible::Degradaci\u00f3 ambiental::Contaminaci\u00f3 atmosf\u00e8rica", ":Desenvolupament hum\u00e0 i sostenible::Degradaci\u00f3 ambiental::Contaminaci\u00f3 atmosf\u00e8rica [\u00c0rees tem\u00e0tiques de la UPC]", "Air pollutants"]}, "links": [{"href": "https://essd.copernicus.org/articles/13/367/2021/essd-13-367-2021.pdf"}, {"href": "https://doi.org/10.5194/essd-13-367-2021"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Earth%20System%20Science%20Data", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5194/essd-13-367-2021", "name": "item", "description": "10.5194/essd-13-367-2021", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5194/essd-13-367-2021"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-12T00:00:00Z"}}, {"id": "10261/331759", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-23T16:25:41Z", "type": "Journal Article", "created": "2023-05-19", "title": "Fertilization strategies for abating N pollution at the scale of a highly vulnerable and diverse semi-arid agricultural region (Murcia, Spain)", "description": "AbstractOveruse of N fertilizers in crops has induced the disruption of the N cycle, triggering the release of reactive N (Nr) to the environment. Several EU policies have been developed to address this challenge, establishing targets to reduce agricultural Nr losses. Their achievement could be materialized through the introduction of fertilizing innovations such as incorporating fertilizer into soils, using urease inhibitors, or by adjusting N inputs to crop needs that could impact in both yields and environment. The Murcia region (southeastern Spain) was selected as a paradigmatic case study, since overfertilization has induced severe environmental problems in the region in the last decade, to assess the impact of a set of 8 N fertilizing alternatives on crop yields and environmental Nr losses. Some of these practices imply the reduction of N entering in crops. We followed an integrated approach analyzing the evolution of the region in the long-term (1860\uffe2\uff80\uff932018) and considering nested spatial- (from grid to region) and systems scales (from crops to the full agro-food system). We hypothesized that, even despite reduction of N inputs, suitable solutions for the abatement of Nr can be identified without compromising crop yields. The most effective option to reduce Nr losses was removing synthetic N fertilizers, leading to 75% reductions in N surpluses mainly due to a reduction of 64% of N inputs, but with associated yield penalties (31%\uffe2\uff80\uff9335%). The most feasible alternative was the removal of urea, resulting in 19% reductions of N inputs, 15%\uffe2\uff80\uff9321% declines in N surplus, and negligible yield losses. While these measures are applied at the field scale, their potential to produce a valuable change can only be assessed at regional scale. Because of this, a spatial analysis was performed showing that largest Nr losses occurred in irrigated horticultural crops. The policy implications of the results are discussed.Overuse of N fertilizers in crops has induced the disruption of the N cycle, triggering the release of reactive N (Nr) to the environment. Several EU policies have been developed to address this challenge, establishing targets to reduce agricultural Nr losses. Their achievement could be materialized through the introduction of fertilizing innovations such as incorporating fertilizer into soils, using urease inhibitors, or by adjusting N inputs to crop needs that could impact in both yields and environment. The Murcia region (southeastern Spain) was selected as a paradigmatic case study, since overfertilization has induced severe environmental problems in the region in the last decade, to assess the impact of a set of 8 N fertilizing alternatives on crop yields and environmental Nr losses. Some of these practices imply the reduction of N entering in crops. We followed an integrated approach analyzing the evolution of the region in the long-term (1860\uffe2\uff80\uff932018) and considering nested spatial- (from grid to region) and systems scales (from crops to the full agro-food system). We hypothesized that, even despite reduction of N inputs, suitable solutions for the abatement of Nr can be identified without compromising crop yields. The most effective option to reduce Nr losses was removing synthetic N fertilizers, leading to 75% reductions in N surpluses mainly due to a reduction of 64% of N inputs, but with associated yield penalties (31%\uffe2\uff80\uff9335%). The most feasible alternative was the removal of urea, resulting in 19% reductions of N inputs, 15%\uffe2\uff80\uff9321% declines in N surplus, and negligible yield losses. While these measures are applied at the field scale, their potential to produce a valuable change can only be assessed at regional scale. Because of this, a spatial analysis was performed showing that largest Nr losses occurred in irrigated horticultural crops. The policy implications of the results are discussed.Under controlled laboratory conditions, high and low ammonium availability are known to favor soil ammonia-oxidizing bacteria (AOB) and archaea (AOA) communities, respectively. However, whether this niche segregation is maintained under field conditions in terrestrial ecosystems remains unresolved, particularly at the global scale. We hypothesized that perennial vegetation might favor AOB vs. AOA communities compared with adjacent open areas devoid of perennial vegetation (i.e., bare soil) via several mechanisms, including increasing the amount of ammonium in soil. To test this niche-differentiation hypothesis, we conducted a global field survey including 80 drylands from 6 continents. Data supported our hypothesis, as soils collected under plant canopies had higher levels of ammonium, as well as higher richness (number of terminal restriction fragments; T-RFs) and abundance (qPCR amoA genes) of AOB, and lower richness and abundance of AOA, than those collected in open areas located between plant canopies. Some of the reported associations between plant canopies and AOA and AOB communities can be a consequence of the higher organic matter and available N contents found under plant canopies. Other aspects of soils associated with vegetation including shading and microclimatic conditions might also help explain our results. Our findings provide strong evidence for niche differentiation between AOA and AOB communities in drylands worldwide, advancing our understanding of their ecology and biogeography at the global scale.Under controlled laboratory conditions, high and low ammonium availability are known to favor soil ammonia-oxidizing bacteria (AOB) and archaea (AOA) communities, respectively. However, whether this niche segregation is maintained under field conditions in terrestrial ecosystems remains unresolved, particularly at the global scale. We hypothesized that perennial vegetation might favor AOB vs. AOA communities compared with adjacent open areas devoid of perennial vegetation (i.e., bare soil) via several mechanisms, including increasing the amount of ammonium in soil. To test this niche-differentiation hypothesis, we conducted a global field survey including 80 drylands from 6 continents. Data supported our hypothesis, as soils collected under plant canopies had higher levels of ammonium, as well as higher richness (number of terminal restriction fragments; T-RFs) and abundance (qPCR amoA genes) of AOB, and lower richness and abundance of AOA, than those collected in open areas located between plant canopies. Some of the reported associations between plant canopies and AOA and AOB communities can be a consequence of the higher organic matter and available N contents found under plant canopies. Other aspects of soils associated with vegetation including shading and microclimatic conditions might also help explain our results. Our findings provide strong evidence for niche differentiation between AOA and AOB communities in drylands worldwide, advancing our understanding of their ecology and biogeography at the global scale.Unprecedented nitrogen (N) inputs into terrestrial ecosystems have profoundly altered soil N cycling. Ammonia oxidizers and denitrifiers are the main producers of nitrous oxide (N2O), but it remains unclear how ammonia oxidizer and denitrifier abundances will respond to N loading and whether their responses can predict N\uffe2\uff80\uff90induced changes in soil N2O emission. By synthesizing 101 field studies worldwide, we showed that N loading significantly increased ammonia oxidizer abundance by 107% and denitrifier abundance by 45%. The increases in both ammonia oxidizer and denitrifier abundances were primarily explained by N loading form, and more specifically, organic N loading had stronger effects on their abundances than mineral N loading. Nitrogen loading increased soil N2O emission by 261%, whereas there was no clear relationship between changes in soil N2O emission and shifts in ammonia oxidizer and denitrifier abundances. Our field\uffe2\uff80\uff90based results challenge the laboratory\uffe2\uff80\uff90based hypothesis that increased ammonia oxidizer and denitrifier abundances by N loading would directly cause higher soil N2O emission. Instead, key abiotic factors (mean annual precipitation, soil pH, soil C:N ratio, and ecosystem type) explained N\uffe2\uff80\uff90induced changes in soil N2O emission. Altogether, these findings highlight the need for considering the roles of key abiotic factors in regulating soil N transformations under N loading to better understand the microbially mediated soil N2O emission. Extensive application of clustering and classification algorithms shows the potential of a CNT-based sensor array in breathomics.
Anthropogenic nitrogen (N) loading alters soil ammonia\uffe2\uff80\uff90oxidizing archaea (AOA) and bacteria (AOB) abundances, likely leading to substantial changes in soil nitrification. However, the factors and mechanisms determining the responses of soil AOA:AOB and nitrification to N loading are still unclear, making it difficult to predict future changes in soil nitrification. Herein, we synthesize\uffc2\uffa068 field studies around the world to evaluate the impacts of N loading on soil ammonia oxidizers and nitrification. Across a wide range of biotic and abiotic factors, climate is the most important driver of the responses of AOA:AOB to N loading. Climate does not directly affect the N\uffe2\uff80\uff90stimulation of nitrification, but does so via climate\uffe2\uff80\uff90related shifts in AOA:AOB. Specifically, climate modulates the responses of AOA:AOB to N loading by affecting soil pH, N\uffe2\uff80\uff90availability and moisture. AOB play a dominant role in affecting nitrification in dry climates, while the impacts from AOA can exceed AOB in humid climates. Together, these results suggest that climate\uffe2\uff80\uff90related shifts in soil ammonia\uffe2\uff80\uff90oxidizing community maintain the N\uffe2\uff80\uff90stimulation of nitrification, highlighting the importance of microbial community composition in mediating the responses of the soil N cycle to N loading. Nitrification is a ubiquitous microbially mediated process in the environment and an essential process in engineered systems such as wastewater and drinking water treatment plants. However, nitrification also contributes to fertilizer loss from agricultural environments, increasing the eutrophication of downstream aquatic ecosystems, and produces the greenhouse gas nitrous oxide. As ammonia-oxidizing bacteria are the most dominant ammonia-oxidizing microbes in fertilized agricultural soils, understanding their responses to a variety of environmental conditions is essential for curbing the negative environmental effects of nitrification. Notably, oxygen limitation has been reported to significantly increase nitric oxide and nitrous oxide production during nitrification. Here, we investigate the physiology of the best-characterized ammonia-oxidizing bacterium, Nitrosomonas europaea , growing under oxygen-limited conditions. Alterations of hydrogen peroxide (H2O2) levels have a profound impact on numerous signaling cascades orchestrating plant growth, development, and stress signaling, including programmed cell death. To expand the repertoire of known molecular mechanisms implicated in H2O2 signaling, we performed a forward chemical screen to identify small molecules that could alleviate the photorespiratory-induced cell death phenotype of Arabidopsisthaliana mutants lacking H2O2-scavenging capacity by peroxisomal catalase2. Here, we report the characterization of pakerine, an m-sulfamoyl benzamide from the sulfonamide family. Pakerine alleviates the cell death phenotype of cat2 mutants exposed to photorespiration-promoting conditions and delays dark-induced senescence in wild-type Arabidopsis leaves. By using a combination of transcriptomics, metabolomics, and affinity purification, we identified abnormal inflorescence meristem 1 (AIM1) as a putative protein target of pakerine. AIM1 is a 3-hydroxyacyl-CoA dehydrogenase involved in fatty acid \u03b2-oxidation that contributes to jasmonic acid (JA) and salicylic acid (SA) biosynthesis. Whereas intact JA biosynthesis was not required for pakerine bioactivity, our results point toward a role for \u03b2-oxidation-dependent SA production in the execution of H2O2-mediated cell death. Natural nitrogen isotopic signature reveals deprotonation during ammonium transport across living organisms. Abstract. We present the Copernicus Atmosphere Monitoring Service TEMPOral profiles (CAMS-TEMPO), a dataset of global and European emission temporal profiles that provides gridded monthly, daily, weekly and hourly weight factors for atmospheric chemistry modelling. CAMS-TEMPO includes temporal profiles for the priority air pollutants (NOx; SOx; NMVOC, non-methane volatile organic compound; NH3; CO; PM10; and PM2.5) and the greenhouse gases (CO2 and CH4) for each of the following anthropogenic source categories: energy industry (power plants), residential combustion, manufacturing industry, transport (road traffic and air traffic in airports) and agricultural activities (fertilizer use and livestock). The profiles are computed on a global 0.1\u2009\u00d7\u20090.1\u2218 and regional European 0.1\u2009\u00d7\u20090.05\u2218 grid following the domain and sector classification descriptions of the global and regional emission inventories developed under the CAMS programme. The profiles account for the variability of the main emission drivers of each sector. Statistical information linked to emission variability (e.g. electricity production and traffic counts) at national and local levels were collected and combined with existing meteorology-dependent parametrizations to account for the influences of sociodemographic factors and climatological conditions. Depending on the sector and the temporal resolution (i.e. monthly, weekly, daily and hourly) the resulting profiles are pollutant-dependent, year-dependent (i.e. time series from 2010 to 2017) and/or spatially dependent (i.e. the temporal weights vary per country or region). We provide a complete description of the data and methods used to build the CAMS-TEMPO profiles, and whenever possible, we evaluate the representativeness of the proxies used to compute the temporal weights against existing observational data. We find important discrepancies when comparing the obtained temporal weights with other currently used datasets. The CAMS-TEMPO data product including the global (CAMS-GLOB-TEMPOv2.1, https://doi.org/10.24380/ks45-9147, Guevara et al., 2020a) and regional European (CAMS-REG-TEMPOv2.1, https://doi.org/10.24380/1cx4-zy68, Guevara et al., 2020b) temporal profiles are distributed from the Emissions of atmospheric Compounds and Compilation of Ancillary Data (ECCAD) system (https://eccad.aeris-data.fr/, last access: February 2021).