{"type": "FeatureCollection", "features": [{"id": "10.1016/j.envint.2020.106190", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:16:39Z", "type": "Journal Article", "created": "2020-10-26", "title": "Treated wastewater irrigation promotes the spread of antibiotic resistance into subsoil pore-water", "description": "In the present study, we investigated the impact of treated wastewater (TWW) irrigation on the prevalence of antibiotic resistance genes (ARGs) in subsoil pore-water, a so-far under-appreciated matrix. We hypothesized that TWW irrigation increases ARG prevalence in subsoil pore-water. This hypothesis was tested using a multiphase approach, which consisted of sampling percolated subsoil pore-water from lysimeter-wells of a real-scale TWW-irrigated field, operated for commercial farming practices, and controlled, laboratory microcosms irrigated with freshwater or TWW. We monitored the abundance of six selected ARGs (sul1, blaOXA-58, tetM, qnrS, blaCTX-M-32 and blaTEM), the intI1 gene associated with mobile genetic elements and an indicator for anthropogenic pollution and bacterial abundance (16S rRNA gene) by qPCR. The bacterial load of subsoil pore water was independent of both, irrigation intensity in the field study and irrigation water type in the microcosms. Among the tested genes in the field study, sul1 and intI1 exhibited constantly higher relative abundances. Their abundance was further positively correlated with increasing irrigation intensity. Controlled microcosm experiments verified the observed field study results: the relative abundance of several genes, including sul1 and intI1, increased significantly when irrigating with TWW compared to freshwater irrigation. Overall, TWW irrigation promoted the spread of ARGs and intI1 in the subsoil pore-water, while the bacterial load was maintained. The combined results from the real-scale agricultural field and the controlled lab microcosms indicate that the dissemination of ARGs in various subsurface environments needs to be taken into account during TWW irrigation scenarios.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Agricultural Irrigation", "Antibiotic resistance", "Water", "Subsoil pore-water", "Drug Resistance", " Microbial", "Wastewater", "Wastewater reuse", "01 natural sciences", "6. Clean water", "Anti-Bacterial Agents", "Environmental sciences", "qPCR", "Soil", "03 medical and health sciences", "Genes", " Bacterial", "RNA", " Ribosomal", " 16S", "ARGs", "GE1-350", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envint.2020.106190"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environment%20International", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envint.2020.106190", "name": "item", "description": "10.1016/j.envint.2020.106190", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envint.2020.106190"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "oai:idus.us.es:11441/146637", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:36:33Z", "type": "Report", "title": "The global distribution and environmental drivers of the soil antibiotic resistome", "description": "Atribuci\u00f3n 4.0 InternacionalGerman Research Foundation FZT 118", "keywords": ["Antibiotic resistance", "Mobile genetic elements", "Human health", "Global scale", "Global change"], "contacts": [{"organization": "Delgado Baquerizo, Manuel, Hu, Hang Wei, Maestre, Fernando T., Guerra, Carlos A., Eisenhauer, Nico, Eldridge, David J., Blanco-Pastor, Jos\u00e9 Luis, He, Ji Zheng,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/oai:idus.us.es:11441/146637"}, {"rel": "self", "type": "application/geo+json", "title": "oai:idus.us.es:11441/146637", "name": "item", "description": "oai:idus.us.es:11441/146637", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/oai:idus.us.es:11441/146637"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-01T00:00:00Z"}}, {"id": "10.1007/s00253-020-10982-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:14:26Z", "type": "Journal Article", "created": "2020-11-11", "title": "Living with sulfonamides: a diverse range of mechanisms observed in bacteria", "description": "Sulfonamides are the oldest class of synthetic antibiotics still in use in clinical and veterinary settings. The intensive utilization of sulfonamides has been leading to the widespread contamination of the environment with these xenobiotic compounds. Consequently, in addition to pathogens and commensals, also bacteria inhabiting a wide diversity of environmental compartments have been in contact with sulfonamides for almost 90\u00a0years. This review aims at giving an overview of the effect of sulfonamides on bacterial cells, including the strategies used by bacteria to cope with these bacteriostatic agents. These include mechanisms of antibiotic resistance, co-metabolic transformation, and partial or total mineralization of sulfonamides. Possible implications of these mechanisms on the ecosystems and dissemination of antibiotic resistance are also discussed. KEY POINTS: \u2022 Sulfonamides are widespread xenobiotic pollutants; \u2022 Target alteration is the main sulfonamide resistance mechanism observed in bacteria; \u2022 Sulfonamides can be modified, degraded, or used as nutrients by some bacteria.", "keywords": ["0301 basic medicine", "Sulfonamides", "0303 health sciences", "Bacteria", "Antibiotic resistance", "Drug Resistance", " Microbial", "Anti-Bacterial Agents", "3. Good health", "03 medical and health sciences", "Biodegradation", " Environmental", "Biodegradation", "Xenobiotic", "Biotransformation", "Ecosystem"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s00253-020-10982-5.pdf"}, {"href": "https://doi.org/10.1007/s00253-020-10982-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Microbiology%20and%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00253-020-10982-5", "name": "item", "description": "10.1007/s00253-020-10982-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00253-020-10982-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-11-11T00:00:00Z"}}, {"id": "10.1016/j.ijheh.2019.01.004", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:17:07Z", "type": "Journal Article", "created": "2019-02-06", "title": "The impact of on-site hospital wastewater treatment on the downstream communal wastewater system in terms of antibiotics and antibiotic resistance genes", "description": "This study quantified antibiotic and antibiotic resistance gene (ARG) concentrations in hospital and communal wastewaters as well as the influents and effluents of the receiving urban wastewater treatment plants (UWWTP) in two Dutch cities. In only one city, hospital wastewater was treated on-site using advanced technologies, including membrane bioreactor treatment (MBR), ozonation, granulated activated carbon (GAC) and UV-treatment. On-site hospital wastewater (HWW) treatment reduced gene presence of hospital-related antibiotic resistance genes and antibiotic concentrations in the receiving urban wastewater treatment plant. These findings support the need for on-site treatment of high-risk point sources of antibiotic resistance genes. 13 antibiotic resistance genes, Integrase Class 1 and 16S rRNA concentrations were quantified using multiplex quantitative real-time PCR (qPCR) assays and the presence and/or concentration of 711 antibiotics were analyzed. Hospital wastewater contained approximately 25% more antibiotics and gene concentrations between 0.4 log to 1.8-fold higher than communal wastewater (CWW). blaKPC and vanA could be identified as hospital-related genes and were reduced to under the limit of detection (LOD) during on-site treatment. Advanced on-site treatment removed between 0.5 and 3.6-fold more genes than conventional biological urban wastewater treatment (activated sludge). Advanced on-site treatment was able to eliminate 12 out of 19 detected antibiotics, while urban waste water treatment eliminated up to 1 (out of 21 detected). Different advanced treatment technologies were able to target different pollutants to varying extents, making sequential alignment more effective. MBR treatment was most efficient in antibiotic resistance gene reduction and ozonation in antibiotic reduction. blaKPC could only be detected in the influent of the urban wastewater treatment plant receiving untreated hospital wastewater. Similarly, vanA was only consistently detected in this treatment plant. These results indicate a positive effect of on-site treatment of hospital wastewater on the communal sewage system.", "keywords": ["0301 basic medicine", "0303 health sciences", "Drug Resistance", " Microbial", "Wastewater", "Waste Disposal", " Fluid", "Hospitals", "6. Clean water", "Anti-Bacterial Agents", "12. Responsible consumption", "3. Good health", "03 medical and health sciences", "Genes", " Bacterial", "13. Climate action", "11. Sustainability", "Water Pollutants", "Advanced wastewater treatment Contaminants of emerging concern Pharmafilter Antibiotic resistance"]}, "links": [{"href": "https://doi.org/10.1016/j.ijheh.2019.01.004"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Hygiene%20and%20Environmental%20Health", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijheh.2019.01.004", "name": "item", "description": "10.1016/j.ijheh.2019.01.004", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijheh.2019.01.004"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-01T00:00:00Z"}}, {"id": "10.1016/j.envint.2018.03.044", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:16:39Z", "type": "Journal Article", "created": "2018-04-06", "title": "Antibiotic resistance in wastewater treatment plants: Tackling the black box", "description": "Wastewater is among the most important reservoirs of antibiotic resistance in urban environments. The abundance of carbon sources and other nutrients, a variety of possible electron acceptors such as oxygen or nitrate, the presence of particles onto which bacteria can adsorb, or a fairly stable pH and temperature are examples of conditions favouring the remarkable diversity of microorganisms in this peculiar habitat. The wastewater microbiome brings together bacteria of environmental, human and animal origins, many harbouring antibiotic resistance genes (ARGs). Although numerous factors contribute, mostly in a complex interplay, for shaping this microbiome, the effect of specific potential selective pressures such as antimicrobial residues or metals, is supposedly determinant to dictate the fate of antibiotic resistant bacteria (ARB) and ARGs during wastewater treatment. This paper aims to enrich the discussion on the ecology of ARB&ARGs in urban wastewater treatment plants (UWTPs), intending to serve as a guide for wastewater engineers or other professionals, who may be interested in studying or optimizing the wastewater treatment for the removal of ARB&ARGs. Fitting this aim, the paper overviews and discusses: i) aspects of the complexity of the wastewater system and/or treatment that may affect the fate of ARB&ARGs; ii) methods that can be used to explore the resistome, meaning the whole ARB&ARGs, in wastewater habitats; and iii) some frequently asked questions for which are proposed addressing modes. The paper aims at contributing to explore how ARB&ARGs behave in UWTPs having in mind that each plant is a unique system that will probably need a specific procedure to maximize ARB&ARGs removal.", "keywords": ["0301 basic medicine", "Bacteria", "Microbiota", "SWOT analysis", "Wastewater", "15. Life on land", "Wastewater treatment optimization", "01 natural sciences", "6. Clean water", "Water Purification", "12. Responsible consumption", "03 medical and health sciences", "Anti-Infective Agents", "13. Climate action", "Drug Resistance", " Bacterial", "11. Sustainability", "Animals", "Humans", "Antibiotic resistance monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envint.2018.03.044"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environment%20International", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envint.2018.03.044", "name": "item", "description": "10.1016/j.envint.2018.03.044", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envint.2018.03.044"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-06-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2022.120873", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:16:41Z", "type": "Journal Article", "created": "2022-12-15", "title": "Comparative selective pressure potential of antibiotics in the environment", "description": "To guide both environmental and public health policy, it is important to assess the degree of antibiotic resistance selection pressure under measured environmental concentrations (MECs), and to compare the efficacy of different mitigation strategies to minimize the spread of resistance. To this end, the resistance selection and enrichment potential due to antibiotic emissions into the environment must be analysed from a life cycle perspective, for a wide range of antibiotics, and considering variations in the underlying fitness costs between different resistance mutations and genes. The aim of this study is to consistently derive fitness cost-dependent minimum selective concentrations (MSCs) from readily available bacterial inhibition data and to build MSC-based species sensitivity distributions (SSDs). These are then used to determine antibiotic-specific resistance selection concentrations predicted to promote resistance in 5% of exposed bacterial species (RSC5). Using a previously developed competition model, we provide estimated MSC10 endpoints for 2,984 antibiotic and bacterial species combinations; the largest set of modelled MSCs available to date. Based on constructed SSDs, we derive RSC5 for 128 antibiotics with four orders of magnitude difference in their 'selective pressure potential' in the environment. By comparing our RSC5 to MECs, we highlight specific environmental compartments (e.g. hospital and wastewater effluents, lakes and rivers), as well as several antibiotics (e.g. ciprofloxacin, norfloxacin, enrofloxacin, and tetracycline), to be scrutinized for their potential role in resistance selection and dissemination. In addition to enabling comparative risk screening of the selective pressure potential of multiple antibiotics, our SSD-derived RSC5 provide the point of departure for calculating new life cycle-based characterization factors for antibiotics to compare mitigation strategies, thereby contributing towards a 'One-Health' approach to tackling the global antibiotic resistance crisis.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Bacteria", "Antibiotic resistance", "Drug Resistance", " Microbial", "Tetracycline", "6. Clean water", "Anti-Bacterial Agents", "3. Good health", "Selection coefficient", "03 medical and health sciences", "Ciprofloxacin", "Species sensitivity distribution", "Fitness cost", "Life cycle impact assessment", "/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being; name=SDG 3 - Good Health and Well-being", "Minimum selective concentration", "Norfloxacin"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2022.120873"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2022.120873", "name": "item", "description": "10.1016/j.envpol.2022.120873", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2022.120873"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-02-01T00:00:00Z"}}, {"id": "10.1016/j.envres.2019.108608", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:16:42Z", "type": "Journal Article", "created": "2019-07-26", "title": "Antibiotic resistance gene distribution in agricultural fields and crops. A soil-to-food analysis", "description": "Despite the social concern about the generalization of antibiotic resistance hotspots worldwide, very little is known about the contribution of different potential sources to the global risk. Here we present a quantitative analysis of the distribution of Antibiotic Resistance Genes (ARGs) in soil, rhizospheric soil, roots, leaves and beans in tomato, lettuce and broad beans crops (165 samples in total), grown in nine commercial plots distributed in four geographical zones in the vicinity of Barcelona (North East Spain). We also analyzed five soil samples from a nearby forest, with no record of agricultural activities. DNA samples were analyzed for their content in the ARGs sul1, tetM, qnrS1, blaCTX-M-32, blaOXA-58, mecA, and blaTEM, plus the integron intI1, using qPCR methods. In addition, soil microbiomes from the different plots were analyzed by amplicon-targeted 16S rRNA gene sequencing. Our data show a decreasing gradient of ARG loads from soil to fruits and beans, the latter showing only from 0.1 to 0.01% of the abundance values in soil. The type of crop was the main determinant for both ARG distribution and microbiome composition among the different plots, with minor contributions of geographic location and irrigation water source. We propose that soil amendment and/or fertilization, more than irrigation water, are the main drivers of ARG loads on the edible parts of the crop, and that they should therefore be specifically controlled.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Microbiomes", "Agriculture", "Drug Resistance", " Microbial", "Irrigation water", "15. Life on land", "01 natural sciences", "6. Clean water", "Anti-Bacterial Agents", "3. Good health", "qPCR", "Soil", "03 medical and health sciences", "Antibiotic resistance genes", "Genes", " Bacterial", "Spain", "RNA", " Ribosomal", " 16S", "Rhizosphere", "Endophytes", "Food Analysis", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envres.2019.108608"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envres.2019.108608", "name": "item", "description": "10.1016/j.envres.2019.108608", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envres.2019.108608"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-10-01T00:00:00Z"}}, {"id": "10.1016/j.jece.2018.02.022", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:17:12Z", "type": "Journal Article", "created": "2018-02-14", "title": "Inter-laboratory calibration of quantitative analyses of antibiotic resistance genes", "description": "Backgrounds: Antibiotic resistant bacteria and antibiotic resistance genes (ARGs) are major human-health threats, widely distributed in the environment. Quantitative PCR (qPCR) is a standard approach to detect and quantify ARGs in environmental compartments. However, the comparison of gene quantification reported by different laboratories is challenging since data are predominantly obtained under non- harmonized conditions, using different qPCR protocols. Objectives: The aim of this study was to develop and calibrate standardized qPCR procedures for quantification of key ARGs, analyzing the same samples with common protocols and distinct equipment, reagents batches and operators. Methods: Treated wastewater from three European countries were processed immediately after collection and transported to the laboratory for total DNA extraction. DNA extracts from each sample were pooled and aliquots were distributed by five partners involved in the calibration procedure. The genes 16S rRNA, vanA, blaTEM, qnrS, sul1, blaCTXM-32 and intI1 were analyzed using harmonized qPCR protocols and the constructed pNORM1 plasmid, which contains fragments of the seven targeted genes, was used for generating standard curves. Conclusions: The 16S rRNA gene was the most abundant, followed by sul1, intI1, qnrS and blaTEM. Quantifications made by different partners were reproducible and inter-laboratory variation was &lt; 20%. The notorious exception was for the qnrS gene, and therefore protocol improvement is recommended. The genes blaCTXM-32 and vanA were below the limit of quantification in most or all of the samples analyzed. The inter-laboratory calibration is an adequate approach to reliably assess ARG abundance and environmental contamination in different environments and geographic locations.", "keywords": ["Life sciences; biology", "info:eu-repo/classification/ddc/570", "0301 basic medicine", "570", "biology", "Inter-laboratory calibration", "Antibiotic resistance gene", "Wastewater", "Life sciences", "01 natural sciences", "6. Clean water", "3. Good health", "Quantitative PCR", "03 medical and health sciences", "ddc:570", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.jece.2018.02.022"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Chemical%20Engineering", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jece.2018.02.022", "name": "item", "description": "10.1016/j.jece.2018.02.022", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jece.2018.02.022"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-02-01T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2024.134885", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:17:16Z", "type": "Journal Article", "created": "2024-06-12", "title": "Anthropogenic pollution may enhance natural transformation in water, favouring the spread of antibiotic resistance genes", "description": "Aquatic ecosystems are crucial in the antimicrobial resistance cycle. While intracellular DNA has been extensively studied to understand human activity's impact on antimicrobial resistance gene (ARG) dissemination, extracellular DNA is frequently overlooked. This study examines the effect of anthropogenic water pollution on microbial community diversity, the resistome, and ARG dissemination. We analyzed intracellular and extracellular DNA from wastewater treatment plant effluents and lake surface water by shotgun sequencing. We also conducted experiments to evaluate anthropogenic pollution's effect on transforming extracellular DNA (using Gfp-plasmids carrying ARGs) within a natural microbial community. Chemical analysis showed treated wastewater had higher anthropogenic pollution-related parameters than lake water. The richness of microbial community, antimicrobial resistome, and high-risk ARGs was greater in treated wastewaters than in lake waters both for intracellular and extracellular DNA. Except for the high-risk ARGs, richness was significantly higher in intracellular than in extracellular DNA. Several ARGs were associated with mobile genetic elements and located on plasmids. Furthermore, Gfp-plasmid transformation within a natural microbial community was enhanced by anthropogenic pollution levels. Our findings underscore anthropogenic pollution's pivotal role in shaping microbial communities and their antimicrobial resistome. Additionally, it may facilitate ARG dissemination through extracellular DNA plasmid uptake.", "keywords": ["Bacteria", "Antibiotic resistance", "Microbiota", "Water Pollution", "Metagenome assembled genomes", "Drug Resistance", " Microbial", "Horizontal gene transfer", "Wastewater", "extracellular DNA; antibiotic resistance; metagenome assembled genomes; transformation; horizontal gene transfer", "Transformation", "Anti-Bacterial Agents", "Lakes", "Extracellular DNA", "Genes", " Bacterial", "Drug Resistance", " Bacterial", "Water Microbiology", "Plasmids"]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/1115155/2/Sivalingam%20et%20al%202024.pdf"}, {"href": "https://doi.org/10.1016/j.jhazmat.2024.134885"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2024.134885", "name": "item", "description": "10.1016/j.jhazmat.2024.134885", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2024.134885"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2018.10.268", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:17:30Z", "type": "Journal Article", "created": "2018-10-22", "title": "Antibiotic resistance genes distribution in microbiomes from the soil-plant-fruit continuum in commercial Lycopersicon esculentum fields under different agricultural practices", "description": "While the presence of antibiotic resistance genes (ARGs) in agricultural soils and products has been firmly established, their distribution among the different plant parts and the contribution of agricultural practices, including irrigation with reclaimed water, have not been adequately addressed yet. To this end, we analyzed the levels of seven ARGs (sul1, blaTEM, blaCTX-M-32, mecA, qnrS1, tetM, blaOXA-58), plus the integrase gene intl1, in soils, roots, leaves, and fruits from two commercial tomato fields irrigated with either unpolluted groundwater or from a channel impacted by treated wastewater, using culture-independent, quantitative real-time PCR methods. ARGs and intl1 sequences were found in leaves and fruits at levels representing from 1 to 10% of those found in roots or soil. The relative abundance of intl1 sequences correlated with tetM, blaTEM, and sul1 levels, suggesting a high horizontal mobility potential for these ARGs. High-throughput 16S rDNA sequencing revealed microbiome differences both between sample types (soil plus roots versus leaves plus fruits) and sampling zones, and a correlation between the prevalence of Pseudomonadaceae and the levels of different ARGs, particularly in fruits and leaves. We concluded that both microbiome composition and ARGs levels in plants parts, including fruits, were likely influenced by agricultural practices.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Antibiotic resistance", "Microbiota", "Microbiomes", "Agriculture", "Drug Resistance", " Microbial", "Horizontal gene transfer", "Irrigation water", "15. Life on land", "6. Clean water", "qPCR", "Soil", "03 medical and health sciences", "Solanum lycopersicum", "Genes", " Bacterial", "Fruit", "Soil Microbiology", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2018.10.268"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2018.10.268", "name": "item", "description": "10.1016/j.scitotenv.2018.10.268", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2018.10.268"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-01T00:00:00Z"}}, {"id": "10.1016/j.watres.2017.10.007", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:18:07Z", "type": "Journal Article", "created": "2017-10-27", "title": "The role of operating parameters and oxidative damage mechanisms of advanced chemical oxidation processes in the combat against antibiotic-resistant bacteria and resistance genes present in urban wastewater", "description": "An upsurge in the study of antibiotic resistance in the environment has been observed in the last decade. Nowadays, it is becoming increasingly clear that urban wastewater is a key source of antibiotic resistance determinants, i.e. antibiotic-resistant bacteria and antibiotic resistance genes (ARB&ARGs). Urban wastewater reuse has arisen as an important component of water resources management in the European Union and worldwide to address prolonged water scarcity issues. Especially, biological wastewater treatment processes (i.e. conventional activated sludge), which are widely applied in urban wastewater treatment plants, have been shown to provide an ideal environment for the evolution and spread of antibiotic resistance. The ability of advanced chemical oxidation processes (AOPs), e.g. light-driven oxidation in the presence of H2O2, ozonation, homogeneous and heterogeneous photocatalysis, to inactivate ARB and remove ARGs in wastewater effluents has not been yet evaluated through a systematic and integrated approach. Consequently, this review seeks to provide an extensive and critical appraisal on the assessment of the efficiency of these processes in inactivating ARB and removing ARGs in wastewater effluents, based on recent available scientific literature. It tries to elucidate how the key operating conditions may affect the process efficiency, while pinpointing potential areas for further research and major knowledge gaps which need to be addressed. Also, this review aims at shedding light on the main oxidative damage pathways involved in the inactivation of ARB and removal of ARGs by these processes. In general, the lack and/or heterogeneity of the available scientific data, as well as the different methodological approaches applied in the various studies, make difficult the accurate evaluation of the efficiency of the processes applied. Besides the operating conditions, the variable behavior observed by the various examined genetic constituents of the microbial community, may be directed by the process distinct oxidative damage mechanisms in place during the application of each treatment technology. For example, it was shown in various studies that the majority of cellular damage by advanced chemical oxidation may be on cell wall and membrane structures of the targeted bacteria, leaving the internal components of the cells relatively intact/able to repair damage. As a result, further in-depth mechanistic studies are required, to establish the optimum operating conditions under which oxidative mechanisms target internal cell components such as genetic material and ribosomal structures more intensively, thus conferring permanent damage and/or death and preventing potential post-treatment re-growth.", "keywords": ["Titanium", "Photolysis", "Bacteria", "Sewage", "Sulfates", "Ultraviolet Rays", "0211 other engineering and technologies", "Drug Resistance", " Microbial", "Hydrogen Peroxide", "02 engineering and technology", "Wastewater", "Oxidants", "01 natural sciences", "6. Clean water", "Water Purification", "12. Responsible consumption", "Oxidative Stress", "Ozone", "Genes", " Bacterial", "13. Climate action", "Antibiotic resistance Advanced chemical oxidation Inactivation mechanisms Wastewater treatment", "Drug Resistance", " Bacterial", "11. Sustainability", "Oxidation-Reduction", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.watres.2017.10.007"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2017.10.007", "name": "item", "description": "10.1016/j.watres.2017.10.007", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2017.10.007"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-02-01T00:00:00Z"}}, {"id": "10.1016/j.watres.2019.05.025", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:18:07Z", "type": "Journal Article", "created": "2019-05-09", "title": "Continuous ozonation of urban wastewater: Removal of antibiotics, antibiotic-resistant Escherichia coli and antibiotic resistance genes and phytotoxicity", "description": "This work evaluated the removal of a mixture of eight antibiotics (i.e. ampicillin (AMP), azithromycin (AZM), erythromycin (ERY), clarithromycin (CLA), ofloxacin (OFL), sulfamethoxazole (SMX), trimethoprim (TMP) and tetracycline (TC)) from urban wastewater, by ozonation operated in continuous mode at different hydraulic retention times (HRTs) (i.e. 10, 20, 40 and 60\u202fmin) and specific ozone doses (i.e. 0.125, 0.25, 0.50 and 0.75 gO3 gDOC- 1). As expected, the efficiency of ozonation was highly ozone dose- and contact time-dependent. The removal of the parent compounds of the selected antibiotics to levels below their detection limits was achieved with HRT of 40\u202fmin and specific ozone dose of 0.125 gO3 gDOC- 1. The effect of ozonation was also investigated at a microbiological and genomic level, by studying the efficiency of the process with respect to the inactivation of Escherichia coli and antibiotic-resistant E.\u00a0coli, as well as to the reduction of the abundance of selected antibiotic resistance genes (ARGs). The inactivation of total cultivable E.\u00a0coli was achieved under the experimental conditions of HRT 40\u202fmin and 0.25 gO3 gDOC-1, at which all antibiotic compounds were already degraded. The regrowth examinations revealed that higher ozone concentrations were required for the permanent inactivation of E.\u00a0coli below the Limit of Quantification (<LOQ\u202f=\u202f0.01\u202fCFU mL- 1). Also, the abundance of the examined ARGs (intl1, aadA1, dfrA1, qacE\u03941 and sul1) was found to decrease with increasing HRT and ozone dose. Despite the fact that the mildest operating parameters were able to eliminate the parent compounds of the tested antibiotics in wastewater effluents, it was clearly demonstrated in this study that higher ozone doses were required in order to confer permanent damage and/or death and prevent potential post-treatment re-growth of both total bacteria and ARB, and to reduce the abundance of ARGs below the LOQ. Interestingly, the mineralization of wastewater, in terms of Dissolved Organic Carbon (DOC) removal, was found to be significantly low even when the higher ozone doses were applied, leading to an increased phytotoxicity towards various plant species. The findings of this study clearly underline the importance of properly optimising the ozonation process (e.g. specific ozone dose and contact time) taking into consideration both the bacterial species and associated ARGs, as well as the wastewater physicochemical properties (e.g. DOC), in order to mitigate the spread of ARB&ARGs, as well as to reduce the potential phytotoxicity.", "keywords": ["Antibiotic resistance", "AntibioticsAntibiotic resistancePhytotoxicityOzonationContinuous mode", "0211 other engineering and technologies", "Drug Resistance", " Microbial", "02 engineering and technology", "Wastewater", "Waste Disposal", " Fluid", "01 natural sciences", "6. Clean water", "Continuous mode", "Anti-Bacterial Agents", "Water Purification", "3. Good health", "Ozone", "Antibiotics", "Ozonation", "Phytotoxicity", "11. Sustainability", "Escherichia coli", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.watres.2019.05.025"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2019.05.025", "name": "item", "description": "10.1016/j.watres.2019.05.025", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2019.05.025"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-08-01T00:00:00Z"}}, {"id": "10.1016/j.watres.2019.114916", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:18:07Z", "type": "Journal Article", "created": "2019-07-25", "title": "Removal of extracellular free DNA and antibiotic resistance genes from water and wastewater by membranes ranging from microfiltration to reverse osmosis", "description": "The final publication is available via https://doi.org/10.1016/j.watres.2019.114916.", "keywords": ["Osmosis", "0211 other engineering and technologies", "membrane filtration", "Wastewater treatment", "02 engineering and technology", "water reuse", "Wastewater", "01 natural sciences", "Water Purification", "12. Responsible consumption", "Water reuse", "antibiotic resistance genes", "free extracellular DNA", "Antibiotic resistance genes", "11. Sustainability", "Humans", "Drinking water treatment", "0105 earth and related environmental sciences", "Water", "Drug Resistance", " Microbial", "DNA", "drinking water treatment", "6. Clean water", "Anti-Bacterial Agents", "wastewater treatment", "Genes", " Bacterial", "Free extracellular DNA", "Membrane filtration"]}, "links": [{"href": "https://doi.org/10.1016/j.watres.2019.114916"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2019.114916", "name": "item", "description": "10.1016/j.watres.2019.114916", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2019.114916"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-11-01T00:00:00Z"}}, {"id": "10.1021/acs.est.0c01565", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:18:14Z", "type": "Journal Article", "created": "2020-05-15", "title": "Changes in Antibiotic Resistance Gene Levels in Soil after Irrigation with Treated Wastewater: A Comparison between Heterogeneous Photocatalysis and Chlorination", "description": "Wastewater (WW) reuse is expected to be increasingly indispensable in future water management to mitigate water scarcity. However, this increases the risk of antibiotic resistance (AR) dissemination via irrigation. Herein, a conventional (chlorination) and an advanced oxidation process (heterogeneous photocatalysis (HPC)) were used to disinfect urban WW to the same target of Escherichia coli <10 CFU/100 mL and used to irrigate lettuce plants (Lactuca sativa) set up in four groups, each receiving one of four water types, secondary WW (positive control), fresh water (negative control), chlorinated WW, and HPC WW. Four genes were monitored in water and soil, 16S rRNA as an indicator of total bacterial load, intI1 as a gene commonly associated with anthropogenic activity and AR, and two AR genes blaOXA-10 and qnrS. Irrigation with secondary WW resulted in higher dry soil levels of intI1 (from 1.4 \u00d7 104 copies/g before irrigation to 3.3 \u00d7 105 copies/g after). HPC-treated wastewater showed higher copy numbers of intI1 in the irrigated soil than chlorination, but the opposite was true for blaOXA-10. The results indicate that the current treatment is insufficient to prevent dissemination of AR markers and that HPC does not offer a clear advantage over chlorination.", "keywords": ["Agricultural Irrigation", "Halogenation", "0211 other engineering and technologies", "Drug Resistance", " Microbial", "02 engineering and technology", "Wastewater", "Waste Disposal", " Fluid", "01 natural sciences", "6. Clean water", "3. Good health", "Soil", "antibiotic resistance; wastewater reuse; photocatalysis; wastewater irrigation", "RNA", " Ribosomal", " 16S", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.iris.unisa.it/bitstream/11386/4749040/1/es-2020-01565f.R1_Proof_hi.pdf"}, {"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.0c01565"}, {"href": "https://doi.org/10.1021/acs.est.0c01565"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.est.0c01565", "name": "item", "description": "10.1021/acs.est.0c01565", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.est.0c01565"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-15T00:00:00Z"}}, {"id": "10.1093/femsec/fiaa058", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:19:30Z", "type": "Journal Article", "created": "2020-03-27", "title": "Persistence of wastewater antibiotic resistant bacteria and their genes in human fecal material", "description": "ABSTRACT<p>Domestic wastewater is a recognized source of antibiotic resistant bacteria and antibiotic resistance genes (ARB&amp;ARGs), whose risk of transmission to humans cannot be ignored. The fitness of wastewater ARB in the complex fecal microbiota of a healthy human was investigated in feces-based microcosm assays (FMAs). FMAs were inoculated with two wastewater isolates, Escherichia coli strain A2FCC14 (MLST ST131) and Enterococcus faecium strain H1EV10 (MLST ST78), harboring the ARGs blaTEM, blaCTX, blaOXA-A and vanA, respectively. The FMAs, incubated in the presence or absence of oxygen or in the presence or absence of the antibiotics cefotaxime or vancomycin, were monitored based on cultivation, ARGs quantification and bacterial community analysis. The fecal bacterial community was dominated by members of the phyla Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria and Verrucomicrobia. The ARGs harbored by the wastewater isolates could be quantified after one week, in FMAs incubated under both aerobic and anaerobic conditions. These observations were not significantly different in FMAs incubated anaerobically, supplemented with sub-inhibitory concentrations of cefotaxime or vancomycin. The observation that ARGs of wastewater ARB persisted in presence of the human fecal microbiota for at least one week supports the hypothesis of a potential transmission to humans, a topic that deserves further investigation.</p>", "keywords": ["0301 basic medicine", "Microcosm assays", "0303 health sciences", "Bacteria", "Angiotensin-Converting Enzyme Inhibitors", "Wastewater", "Human fecal microbiota", "6. Clean water", "Anti-Bacterial Agents", "3. Good health", "Angiotensin Receptor Antagonists", "Feces", "03 medical and health sciences", "Antibiotic resistance genes", "Antibiotic resistant bacteria", "Genes", " Bacterial", "11. Sustainability", "Humans", "Antibiotic resistance transmission", "Microcosm effect", "Multilocus Sequence Typing"]}, "links": [{"href": "http://academic.oup.com/femsec/article-pdf/96/6/fiaa058/33327470/fiaa058.pdf"}, {"href": "https://doi.org/10.1093/femsec/fiaa058"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/femsec/fiaa058", "name": "item", "description": "10.1093/femsec/fiaa058", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/femsec/fiaa058"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-02T00:00:00Z"}}, {"id": "10.1186/s40168-022-01405-w", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:20:43Z", "type": "Journal Article", "created": "2022-12-12", "title": "The global distribution and environmental drivers of the soil antibiotic resistome", "description": "Abstract                 Background                 <p>Little is known about the global distribution and environmental drivers of key microbial functional traits such as antibiotic resistance genes (ARGs). Soils are one of Earth\uffe2\uff80\uff99s largest reservoirs of ARGs, which are integral for soil microbial competition, and have potential implications for plant and human health. Yet, their diversity and global patterns remain poorly described. Here, we analyzed 285 ARGs in soils from 1012 sites across all continents and created the first global atlas with the distributions of topsoil ARGs.</p>                                Results                 <p>We show that ARGs peaked in high latitude cold and boreal forests. Climatic seasonality and mobile genetic elements, associated with the transmission of antibiotic resistance, were also key drivers of their global distribution. Dominant ARGs were mainly related to multidrug resistance genes and efflux pump machineries. We further pinpointed the global hotspots of the diversity and proportions of soil ARGs.</p>                                Conclusions                 <p>Together, our work provides the foundation for a better understanding of the ecology and global distribution of the environmental soil antibiotic resistome.</p>", "keywords": ["Ecolog\u00eda (Biolog\u00eda)", "0301 basic medicine", "SDG-03: Good health and well-being", "550", "Antibiotic resistance", "Edafolog\u00eda (Biolog\u00eda)", "Antibiotic resistance genes (ARGs)", "910", "http://metadata.un.org/sdg/3", "631.4", "Microbial ecology", "2417.14 Gen\u00e9tica Vegetal", "Soil", "03 medical and health sciences", "XXXXXX - Unknown", "Global scale", "Humans", "Global change", "SCALE", "Ensure healthy lives and promote well-being for all at all ages", "2. Zero hunger", "0303 health sciences", "Ecology", "Research", "QR100-130", "Human health", "15. Life on land", "Gen\u00e9tica", "Anti-Bacterial Agents", "3. Good health", "Phenotype", "Mobile genetic elements", "13. Climate action", "BACTERIA", "2511.02 Biolog\u00eda de Suelos", "RESISTANCE GENES"]}, "links": [{"href": "https://doi.org/10.1186/s40168-022-01405-w"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40168-022-01405-w", "name": "item", "description": "10.1186/s40168-022-01405-w", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40168-022-01405-w"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-07-11T00:00:00Z"}}, {"id": "10.3390/catal9030222", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:22:29Z", "type": "Journal Article", "created": "2019-03-04", "title": "Immobilised Cerium-Doped Zinc Oxide as a Photocatalyst for the Degradation of Antibiotics and the Inactivation of Antibiotic-Resistant Bacteria", "description": "<p>The threat of antibiotic resistance to the wellbeing of societies is well established. Urban wastewater treatment plants (UWTPs) are recognised sources for antibiotic resistance dissemination in the environment. Herein a novel cerium-doped zinc oxide (Ce-ZnO) photocatalyst is compared to ZnO and the benchmark TiO2-P25 in the immobilised form on a metallic support, to evaluate a photocatalytic process as a possible tertiary treatment in UWTPs. The catalysts were compared for the removal of two antibiotics, trimethoprim (TMP) and sulfamethoxazole (SMX), and for the inactivation of Escherichia coli (E. coli) strain DH5-Alpha in isotonic sodium chloride solution and of autochthonous bacteria in real secondary wastewater. In real wastewater, E. coli and other coliforms were monitored, as well as the respective fractions resistant to ofloxacin and azithromycin. In parallel, Pseudomonas aeruginosa and the respective sub-population resistant to ofloxacin or ciprofloxacin were also monitored. Photocatalysis with both ZnO and Ce-ZnO was faster than using TiO2-P25 at degrading the antibiotics, with Ce-ZnO the fastest against SMX but slower than undoped ZnO in the removal of TMP. Ce-ZnO catalyst reuse in the immobilised form produced somewhat slower kinetics maintained &gt;50% of the initial activity, even after five cycles of use. Approximately 3 log10 inactivation of E. coli in isotonic sodium chloride water was recorded with reproducible results. In the removal of autochthonous bacteria in real wastewater, Ce-ZnO performed better (more than 2 log values higher) than TiO2-P25. In all cases, E. coli and other coliforms, including their resistant subpopulations, were inactivated at a higher rate than P. aeruginosa. With short reaction times no evidence for enrichment of resistance was observed, yet with extended reaction times low levels of bacterial loads were not further inactivated. Overall, Ce-ZnO is an easy and cheap photocatalyst to produce and immobilise and the one that showed higher activity than the industry standard TiO2-P25 against the tested antibiotics and bacteria, including antibiotic-resistant bacteria.</p>", "keywords": ["tertiary treatment", "wastewater disinfection", "antibiotic resistance", "Antibiotic resistance", "Tertiary treatment", "immobilised photocatalyst", "Immobilised photocatalyst", "02 engineering and technology", "01 natural sciences", "6. Clean water", "3. Good health", "Wastewater disinfection", "13. Climate action", "11. Sustainability", "Antibiotic resistance; Immobilised photocatalyst; Photocatalysis; Tertiary treatment; Wastewater disinfection; Catalysis; Physical and Theoretical Chemistry", "Photocatalysis", "0210 nano-technology", "photocatalysis", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://www.mdpi.com/2073-4344/9/3/222/pdf"}, {"href": "https://www.iris.unisa.it/bitstream/11386/4723219/1/Zammit%20et%20al.%202019_Catalysts.pdf"}, {"href": "https://www.mdpi.com/2073-4344/9/3/222/pdf"}, {"href": "https://doi.org/10.3390/catal9030222"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Catalysts", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/catal9030222", "name": "item", "description": "10.3390/catal9030222", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/catal9030222"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-03-01T00:00:00Z"}}, {"id": "10.3390/su17031093", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:22:48Z", "type": "Journal Article", "created": "2025-01-29", "title": "Microbial Bioindicators for Monitoring the Impact of Emerging Contaminants on Soil Health in the European Framework", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Antibiotic resistance (AR) is recognized by the World Health Organization as a major threat to human health, and recent studies highlight the role of microplastics (MPs) in its spread. MPs in the environment may act as vectors for antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). Bacterial communities on the plastisphere, the surface of MPs, are influenced by plastic properties, allowing ARB to colonize and form biofilms. These biofilms facilitate the transfer of ARGs within microbial communities. This study analyzed data from the LUCAS soil dataset (885 soil samples across EU countries) using the Emu tool to characterize microbial communities at the genus/species level. Functional annotation via PICRUSt2, supported by a custom tool for Emu output formatting, revealed significant correlations between the genera Solirubrobacter, Bradyrhizobium, Nocardioides, and Bacillus with pathways linked to microplastic degradation and antibiotic resistance. These genera were consistently present in various soil types (woodland, grassland, and cropland), suggesting their potential as bioindicators of soil health in relation to MP pollution. The findings underscore MPs as hotspots for ARB and ARGs, offering new insights into the identification of bioindicators for monitoring soil health and the ecological impacts related to MP contamination.</p></article>", "keywords": ["microplastics; antibiotic resistance genes; soil microbiome; LUCAS soil"], "contacts": [{"organization": "Andrea Visca, Luciana Di Gregorio, Manuela Costanzo, Elisa Clagnan, Lorenzo Nolfi, Roberta Bernini, Alberto Orgiazzi, Arwyn Jones, Francesco Vitali, Stefano Mocali, Annamaria Bevivino,", "roles": ["creator"]}]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/1142151/2/sustainability-17-01093.pdf"}, {"href": "https://www.mdpi.com/2071-1050/17/3/1093/pdf"}, {"href": "https://doi.org/10.3390/su17031093"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sustainability", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/su17031093", "name": "item", "description": "10.3390/su17031093", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/su17031093"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-01-29T00:00:00Z"}}, {"id": "11441/146637", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:27:12Z", "type": "Report", "title": "The global distribution and environmental drivers of the soil antibiotic resistome", "description": "Open AccessGerman Research Foundation FZT 118", "keywords": ["Antibiotic resistance", "Mobile genetic elements", "Human health", "Global scale", "Global change"], "contacts": [{"organization": "Delgado Baquerizo, Manuel, Hu, Hang Wei, Maestre, Fernando T., Guerra, Carlos A., Eisenhauer, Nico, Eldridge, David J., Blanco-Pastor, Jos\u00e9 Luis, He, Ji Zheng,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/11441/146637"}, {"rel": "self", "type": "application/geo+json", "title": "11441/146637", "name": "item", "description": "11441/146637", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11441/146637"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-01T00:00:00Z"}}, {"id": "11587/561788", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:27:17Z", "type": "Journal Article", "created": "2025-07-04", "title": "Per- and polyfluoroalkyl substances (PFAS) as environmental drivers of antimicrobial resistance: insights from genome sequences of Klebsiella grimontii and Citrobacter braakii isolated from contaminated soil", "description": "<p>             In vitro microcosm experiments showed that PFOA (perfluorooctanoic acid) selects for antibiotic-resistant bacteria, characterized by WGS, which displayed increased transcription of AMR genes during growth in the presence of PFOA.</p", "keywords": ["PFAS", " Antimicrobial resistance", " Antibiotic resistance genes; Klebsiella grimontii", " Citrobacter braakii"]}, "links": [{"href": "https://doi.org/11587/561788"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%3A%20Advances", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11587/561788", "name": "item", "description": "11587/561788", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11587/561788"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-01-01T00:00:00Z"}}, {"id": "1959.7/uws:76535", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:27:33Z", "type": "Journal Article", "created": "2022-12-11", "title": "The global distribution and environmental drivers of the soil antibiotic resistome", "description": "Abstract                                        Background                     <p>Little is known about the global distribution and environmental drivers of key microbial functional traits such as antibiotic resistance genes (ARGs). Soils are one of Earth\uffe2\uff80\uff99s largest reservoirs of ARGs, which are integral for soil microbial competition, and have potential implications for plant and human health. Yet, their diversity and global patterns remain poorly described. Here, we analyzed 285 ARGs in soils from 1012 sites across all continents and created the first global atlas with the distributions of topsoil ARGs.</p>                                                           Results                     <p>We show that ARGs peaked in high latitude cold and boreal forests. Climatic seasonality and mobile genetic elements, associated with the transmission of antibiotic resistance, were also key drivers of their global distribution. Dominant ARGs were mainly related to multidrug resistance genes and efflux pump machineries. We further pinpointed the global hotspots of the diversity and proportions of soil ARGs.</p>                                                           Conclusions                     <p>Together, our work provides the foundation for a better understanding of the ecology and global distribution of the environmental soil antibiotic resistome.</p>", "keywords": ["Ecolog\u00eda (Biolog\u00eda)", "0301 basic medicine", "SDG-03: Good health and well-being", "550", "Antibiotic resistance", "Edafolog\u00eda (Biolog\u00eda)", "Antibiotic resistance genes (ARGs)", "910", "631.4", "Microbial ecology", "2417.14 Gen\u00e9tica Vegetal", "Soil", "03 medical and health sciences", "XXXXXX - Unknown", "Global scale", "Humans", "Global change", "SCALE", "2. Zero hunger", "0303 health sciences", "Ecology", "Research", "QR100-130", "Human health", "15. Life on land", "Gen\u00e9tica", "Anti-Bacterial Agents", "3. Good health", "Phenotype", "Mobile genetic elements", "13. Climate action", "BACTERIA", "2511.02 Biolog\u00eda de Suelos", "RESISTANCE GENES"]}, "links": [{"href": "https://doi.org/1959.7/uws:76535"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "1959.7/uws:76535", "name": "item", "description": "1959.7/uws:76535", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/1959.7/uws:76535"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-07-11T00:00:00Z"}}, {"id": "2434/1115155", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-04-03T16:28:04Z", "type": "Journal Article", "created": "2024-06-12", "title": "Anthropogenic pollution may enhance natural transformation in water, favouring the spread of antibiotic resistance genes", "description": "Aquatic ecosystems are crucial in the antimicrobial resistance cycle. While intracellular DNA has been extensively studied to understand human activity's impact on antimicrobial resistance gene (ARG) dissemination, extracellular DNA is frequently overlooked. This study examines the effect of anthropogenic water pollution on microbial community diversity, the resistome, and ARG dissemination. We analyzed intracellular and extracellular DNA from wastewater treatment plant effluents and lake surface water by shotgun sequencing. We also conducted experiments to evaluate anthropogenic pollution's effect on transforming extracellular DNA (using Gfp-plasmids carrying ARGs) within a natural microbial community. Chemical analysis showed treated wastewater had higher anthropogenic pollution-related parameters than lake water. The richness of microbial community, antimicrobial resistome, and high-risk ARGs was greater in treated wastewaters than in lake waters both for intracellular and extracellular DNA. Except for the high-risk ARGs, richness was significantly higher in intracellular than in extracellular DNA. Several ARGs were associated with mobile genetic elements and located on plasmids. Furthermore, Gfp-plasmid transformation within a natural microbial community was enhanced by anthropogenic pollution levels. Our findings underscore anthropogenic pollution's pivotal role in shaping microbial communities and their antimicrobial resistome. Additionally, it may facilitate ARG dissemination through extracellular DNA plasmid uptake.", "keywords": ["Bacteria", "Antibiotic resistance", "Microbiota", "Water Pollution", "Metagenome assembled genomes", "Drug Resistance", " Microbial", "Horizontal gene transfer", "Wastewater", "extracellular DNA; antibiotic resistance; metagenome assembled genomes; transformation; horizontal gene transfer", "Transformation", "Anti-Bacterial Agents", "Lakes", "Extracellular DNA", "Genes", " Bacterial", "Drug Resistance", " Bacterial", "Water Microbiology", "Plasmids"]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/1115155/2/Sivalingam%20et%20al%202024.pdf"}, {"href": "https://doi.org/2434/1115155"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2434/1115155", "name": "item", "description": "2434/1115155", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2434/1115155"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-01T00:00:00Z"}}, {"id": "2434/1142151", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:28:04Z", "type": "Journal Article", "created": "2025-01-29", "title": "Microbial Bioindicators for Monitoring the Impact of Emerging Contaminants on Soil Health in the European Framework", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Antibiotic resistance (AR) is recognized by the World Health Organization as a major threat to human health, and recent studies highlight the role of microplastics (MPs) in its spread. MPs in the environment may act as vectors for antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). Bacterial communities on the plastisphere, the surface of MPs, are influenced by plastic properties, allowing ARB to colonize and form biofilms. These biofilms facilitate the transfer of ARGs within microbial communities. This study analyzed data from the LUCAS soil dataset (885 soil samples across EU countries) using the Emu tool to characterize microbial communities at the genus/species level. Functional annotation via PICRUSt2, supported by a custom tool for Emu output formatting, revealed significant correlations between the genera Solirubrobacter, Bradyrhizobium, Nocardioides, and Bacillus with pathways linked to microplastic degradation and antibiotic resistance. These genera were consistently present in various soil types (woodland, grassland, and cropland), suggesting their potential as bioindicators of soil health in relation to MP pollution. The findings underscore MPs as hotspots for ARB and ARGs, offering new insights into the identification of bioindicators for monitoring soil health and the ecological impacts related to MP contamination.</p></article>", "keywords": ["microplastics; antibiotic resistance genes; soil microbiome; LUCAS soil"]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/1142151/2/sustainability-17-01093.pdf"}, {"href": "https://www.mdpi.com/2071-1050/17/3/1093/pdf"}, {"href": "https://doi.org/2434/1142151"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sustainability", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2434/1142151", "name": "item", "description": "2434/1142151", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2434/1142151"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-01-29T00:00:00Z"}}, {"id": "36529346", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:28:56Z", "type": "Journal Article", "created": "2022-12-15", "title": "Comparative selective pressure potential of antibiotics in the environment", "description": "To guide both environmental and public health policy, it is important to assess the degree of antibiotic resistance selection pressure under measured environmental concentrations (MECs), and to compare the efficacy of different mitigation strategies to minimize the spread of resistance. To this end, the resistance selection and enrichment potential due to antibiotic emissions into the environment must be analysed from a life cycle perspective, for a wide range of antibiotics, and considering variations in the underlying fitness costs between different resistance mutations and genes. The aim of this study is to consistently derive fitness cost-dependent minimum selective concentrations (MSCs) from readily available bacterial inhibition data and to build MSC-based species sensitivity distributions (SSDs). These are then used to determine antibiotic-specific resistance selection concentrations predicted to promote resistance in 5% of exposed bacterial species (RSC5). Using a previously developed competition model, we provide estimated MSC10 endpoints for 2,984 antibiotic and bacterial species combinations; the largest set of modelled MSCs available to date. Based on constructed SSDs, we derive RSC5 for 128 antibiotics with four orders of magnitude difference in their 'selective pressure potential' in the environment. By comparing our RSC5 to MECs, we highlight specific environmental compartments (e.g. hospital and wastewater effluents, lakes and rivers), as well as several antibiotics (e.g. ciprofloxacin, norfloxacin, enrofloxacin, and tetracycline), to be scrutinized for their potential role in resistance selection and dissemination. In addition to enabling comparative risk screening of the selective pressure potential of multiple antibiotics, our SSD-derived RSC5 provide the point of departure for calculating new life cycle-based characterization factors for antibiotics to compare mitigation strategies, thereby contributing towards a 'One-Health' approach to tackling the global antibiotic resistance crisis.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Bacteria", "Antibiotic resistance", "Drug Resistance", " Microbial", "Tetracycline", "6. Clean water", "Anti-Bacterial Agents", "3. Good health", "Selection coefficient", "03 medical and health sciences", "Ciprofloxacin", "Species sensitivity distribution", "Fitness cost", "Life cycle impact assessment", "/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being; name=SDG 3 - Good Health and Well-being", "Minimum selective concentration", "Norfloxacin"]}, "links": [{"href": "https://doi.org/36529346"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "36529346", "name": "item", "description": "36529346", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/36529346"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-02-01T00:00:00Z"}}, {"id": "95f3c5f087e174a573456a389625476c", "type": "Feature", "geometry": null, "properties": {"updated": "2026-04-03T16:30:29Z", "type": "Report", "title": "The global distribution and environmental drivers of the soil antibiotic resistome", "description": "Atribuci\u00f3n 4.0 InternacionalGerman Research Foundation FZT 118", "keywords": ["Antibiotic resistance", "Mobile genetic elements", "Human health", "Global scale", "Global change"], "contacts": [{"organization": "Delgado Baquerizo, Manuel, Hu, Hang Wei, Maestre, Fernando T., Guerra, Carlos A., Eisenhauer, Nico, Eldridge, David J., Blanco-Pastor, Jos\u00e9 Luis, He, Ji Zheng,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/95f3c5f087e174a573456a389625476c"}, {"rel": "self", "type": "application/geo+json", "title": "95f3c5f087e174a573456a389625476c", "name": "item", "description": "95f3c5f087e174a573456a389625476c", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/95f3c5f087e174a573456a389625476c"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Antibiotic+resistance&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Antibiotic+resistance&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Antibiotic+resistance&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Antibiotic+resistance&offset=25", "hreflang": "en-US"}], "numberMatched": 25, "numberReturned": 25, "distributedFeatures": [], "timeStamp": "2026-04-04T13:41:16.994472Z"}