{"type": "FeatureCollection", "features": [{"id": "10.1007/s00253-012-4173-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:14:40Z", "type": "Journal Article", "created": "2012-06-20", "title": "Pectin-Rich Biomass As Feedstock For Fuel Ethanol Production", "description": "The USA has proposed that 30\u00a0% of liquid transportation fuel be produced from renewable resources by 2030 (Perlack and Stokes 2011). It will be impossible to reach this goal using corn kernel-based ethanol alone. Pectin-rich biomass, an under-utilized waste product of the sugar and juice industry, can augment US ethanol supplies by capitalizing on this already established feedstock. Currently, pectin-rich biomass is sold (at low value) as animal feed. This review focuses on the three most studied types of pectin-rich biomass: sugar beet pulp, citrus waste and apple pomace. Fermentations of these materials have been conducted with a variety of ethanologens, including yeasts and bacteria. Escherichia coli can ferment a wide range of sugars including galacturonic acid, the primary component of pectin. However, the mixed acid metabolism of E. coli can produce unwanted side products. Saccharomyces cerevisiae cannot naturally ferment galacturonic acid nor pentose sugars but has a homoethanol pathway. Erwinia chrysanthemi is capable of degrading many of the cell wall components of pectin-rich materials, including pectin. Klebsiella oxytoca can metabolize a diverse array of sugars including cellobiose, one degradation product of cellulose. However, both E. chrysanthemi and K. oxytoca produce side products during fermentation, similar to E. coli. Using pectin-rich residues from industrial processes is beneficial because the material is already collected and partially pretreated to facilitate enzymatic deconstruction of the plant cell walls. Using biomass already produced for other purposes is an attractive practice because fewer greenhouse gases (GHG) will be anticipated from land-use changes.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Bacteria", "Ethanol", "Fungi", "Industrial Waste", "Mini-Review", "15. Life on land", "Applied Microbiology and Biotechnology", "7. Clean energy", "12. Responsible consumption", "03 medical and health sciences", "13. Climate action", "Fermentation", "Food Industry", "Pectins", "Biomass", "Biotechnology"], "contacts": [{"organization": "Joy Doran-Peterson, Meredith C. Edwards,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/s00253-012-4173-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Microbiology%20and%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00253-012-4173-2", "name": "item", "description": "10.1007/s00253-012-4173-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00253-012-4173-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-06-14T00:00:00Z"}}, {"id": "10.1007/s13213-014-0889-9", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-27T16:15:33Z", "type": "Journal Article", "created": "2014-04-21", "title": "Influence Of Long-Term Fertilization On Soil Microbial Biomass, Dehydrogenase Activity, And Bacterial And Fungal Community Structure In A Brown Soil Of Northeast China", "description": "In this study, the effect of mineral fertilizer and organic manure were evaluated on soil microbial biomass, dehydrogenase activity, bacterial and fungal community structure in a long-term (33\u00a0years) field experiment. Except for the mineral nitrogen fertilizer (N) treatment, long-term fertilization greatly increased soil microbial biomass carbon (SMBC) and dehydrogenase activity. Organic manure had a significantly greater impact on SMBC and dehydrogenase activity, compared with mineral fertilizers. Bacterial and fungal community structure was analyzed by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). Long-term fertilization increased bacterial and fungal ribotype diversity. Total soil nitrogen (TN) and phosphorus (TP), soil organic carbon (SOC) and available phosphorus (AP) had a similar level of influence on bacterial ribotypes while TN, SOC and AP had a larger influence than alkali-hydrolyzable nitrogen (AHN) on fungal ribotypes. Our results suggested that long-term P-deficiency fertilization can significantly decrease soil microbial biomass, dehydrogenase activity and bacterial diversity. N-fertilizer and SOC have an important influence on bacterial and fungal communities.", "keywords": ["2. Zero hunger", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Original Article", "04 agricultural and veterinary sciences", "15. Life on land", "Applied Microbiology and Biotechnology", "3. Good health"], "contacts": [{"organization": "Hongzhi Bai, Mei Han, Xiaori Han, Yan Wang, Hui Shi, Liu Ning, Luo Peiyu,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/s13213-014-0889-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Annals%20of%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s13213-014-0889-9", "name": "item", "description": "10.1007/s13213-014-0889-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s13213-014-0889-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-04-22T00:00:00Z"}}, {"id": "10.1016/j.cofs.2020.11.012", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:16:11Z", "type": "Journal Article", "created": "2020-12-09", "title": "Environmental microbiome mapping as a strategy to improve quality and safety in the food industry", "description": "In food industries, an environmentally-adapted microbiome can colonize the surfaces of equipment and tools and be transferred to the food product or intermediates of production. These complex microbial consortia may include microbial spoilers, pathogens, as well as beneficial microbes.  Advances in sequencing technologies and metagenomics provide the opportunity to map the environmental microbiome in food industries at an unprecedented depth, highlighting the importance of the resident microbial communities in influencing food quality and safety, as well as the main factors shaping its composition and activities. However, specific technical issues must be considered. Although microbiome mapping in the food industry has the potential to revolutionize food safety and quality management systems, its application as routine practice is still challenging and technical issues limit the exploitation of the powerful information that can be obtained by the application of such state-of-the-art approaches.", "keywords": ["Aurora Universities Network", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "EC", "food industry", "H2020", "food quality", "Applied Microbiology and Biotechnology", "Horizon 2020 Framework Programme", "Innovation action", "food safety", "03 medical and health sciences", "contamination", "13. Climate action", "Metagenomics", "European Commission", "Knowmad Institut", "environmental microbiome", "Food Science"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/828326/1/COFS%2c2021_EnvMapping.pdf"}, {"href": "https://doi.org/10.1016/j.cofs.2020.11.012"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Current%20Opinion%20in%20Food%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.cofs.2020.11.012", "name": "item", "description": "10.1016/j.cofs.2020.11.012", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.cofs.2020.11.012"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-04-01T00:00:00Z"}}, {"id": "10.1093/femsec/fiv066", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:19:04Z", "type": "Journal Article", "created": "2015-06-20", "title": "Effects Of Warming And Drought On Potential N2o Emissions And Denitrifying Bacteria Abundance In Grasslands With Different Land-Use", "description": "Increased warming in spring and prolonged summer drought may alter soil microbial denitrification. We measured potential denitrification activity and denitrifier marker gene abundances (nirK, nirS, nosZ) in grasslands soils in three geographic regions characterized by site-specific land-use indices (LUI) after warming in spring, at an intermediate sampling and after summer drought. Potential denitrification was significantly increased by warming, but did not persist over the intermediate sampling. At the intermediate sampling, the relevance of grassland land-use intensity was reflected by increased potential N2O production at sites with higher LUI. Abundances of total bacteria did not respond to experimental warming or drought treatments, displaying resilience to minor and short-term effects of climate change. In contrast, nirS- and nirK-type denitrifiers were more influenced by drought in combination with LUI and pH, while the nosZ abundance responded to the summer drought manipulation. Land-use was a strong driver for potential denitrification as grasslands with higher LUI also had greater potentials for N2O emissions. We conclude that both warming and drought affected the denitrifying communities and the potential denitrification in grassland soils. However, these effects are overruled by regional and site-specific differences in soil chemical and physical properties which are also related to grassland land-use intensity.", "keywords": ["0301 basic medicine", "570", "UFSP13-8 Global Change and Biodiversity", "Climate Change", "Microbial Consortia", "580 Plants (Botany)", "Nitric Oxide", "142-005 142-005", "Soil", "03 medical and health sciences", "potential N2O emissions", "RNA", " Ribosomal", " 16S", "2402 Applied Microbiology and Biotechnology", "use index", "Soil Microbiology", "2. Zero hunger", "Biodiversity Exploratories", "denitrification", "Bacteria", "2404 Microbiology", "04 agricultural and veterinary sciences", "15. Life on land", "Grassland", "6. Clean water", "Droughts", "land", "climate change", "Genes", " Bacterial", "13. Climate action", "8. Economic growth", "Denitrification", "0401 agriculture", " forestry", " and fisheries", "grassland", "microbial community", "2303 Ecology"]}, "links": [{"href": "https://doi.org/10.1093/femsec/fiv066"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/femsec/fiv066", "name": "item", "description": "10.1093/femsec/fiv066", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/femsec/fiv066"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-06-19T00:00:00Z"}}, {"id": "10.1111/j.1574-6941.2007.00394.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:19:44Z", "type": "Journal Article", "created": "2007-10-19", "title": "Quantitation And Diversity Analysis Of Ruminal Methanogenic Populations In Response To The Antimethanogenic Compound Bromochloromethane", "description": "Methyl coenzyme-M reductase A (mcrA) clone libraries were generated from microbial DNA extracted from the rumen of cattle fed a roughage diet with and without supplementation of the antimethanogenic compound bromochloromethane. Bromochloromethane reduced total methane emissions by c. 30%, with a resultant increase in propionate and branched chain fatty acids. The mcrA clone libraries revealed that Methanobrevibacter spp. were the dominant species identified. A decrease in the incidence of Methanobrevibacter spp. from the clone library generated from bromochloromethane treatment was observed. In addition, a more diverse methanogenic population with representatives from Methanococcales, Methanomicrobiales and Methanosacinales orders was observed for the bromochloromethane library. Sequence data generated from these libraries aided in the design of an mcrA-targeted quantitative PCR (qPCR) assay. The reduction in methane production by bromochloromethane was associated with an average decrease of 34% in the number of methanogenic Archaea when monitored with this qPCR assay. Dissociation curve analysis of mcrA amplicons showed a clear difference in melting temperatures for Methanobrevibacter spp. (80-82 degrees C) and all other methanongens (84-86 degrees C). A decrease in the intensity of the Methanobrevibacter spp. specific peak and an increase for the other peak in the bromochloromethane-treated animals corresponded with the changes within the clone libraries.", "keywords": ["Male", "0301 basic medicine", "Rumen", "Bromochloromethane", "Methanogens", "Molecular Sequence Data", "Euryarchaeota", "Methanobrevibacter", "Polymerase Chain Reaction", "630", "03 medical and health sciences", "2402 Applied Microbiology and Biotechnology", "Animals", "Methyl coenzyme-M reductase", "Phylogeny", "Gene Library", "2. Zero hunger", "0303 health sciences", "Hydrocarbons", " Halogenated", "2404 Microbiology", "Sequence Analysis", " DNA", "mcrA", "qPCR", "DNA", " Archaeal", "Cattle", "Oxidoreductases", "2303 Ecology", "Methane"]}, "links": [{"href": "https://doi.org/10.1111/j.1574-6941.2007.00394.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1574-6941.2007.00394.x", "name": "item", "description": "10.1111/j.1574-6941.2007.00394.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1574-6941.2007.00394.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-12-01T00:00:00Z"}}, {"id": "10.1128/aem.02453-08", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-27T16:19:54Z", "type": "Journal Article", "created": "2009-02-07", "title": "A Vaccine Against Rumen Methanogens Can Alter The Composition Of Archaeal Populations", "description": "ABSTRACT           <p>             The objectives of this study were to formulate a vaccine based upon the different species/strains of methanogens present in sheep intended to be immunized and to determine if a targeted vaccine could be used to decrease the methane output of the sheep. Two 16S rRNA gene libraries were used to survey the methanogenic archaea in sheep prior to vaccination, and methanogens representing five phylotypes were found to account for &gt;52% of the different species/strains of methanogens detected. A vaccine based on a mixture of these five methanogens was then formulated, and 32 sheep were vaccinated on days 0, 28, and 103 with either a control or the anti-methanogen vaccine. Enzyme-linked immunosorbent assay analysis revealed that each vaccination with the anti-methanogen formulation resulted in higher specific immunoglobulin G titers in plasma, saliva, and rumen fluid. Methane output levels corrected for dry-matter intake for the control and treatment groups were not significantly different, and real-time PCR data also indicated that methanogen numbers were not significantly different for the two groups after the second vaccination. However, clone library data indicated that methanogen diversity was significantly greater in sheep receiving the anti-methanogen vaccine and that the vaccine may have altered the composition of the methanogen population. A correlation between 16S rRNA gene sequence relatedness and cross-reactivity for the methanogens (             R             2             = 0.90) also exists, which suggests that a highly specific vaccine can be made to target specific strains of methanogens and that a more broad-spectrum approach is needed for success in the rumen. Our data also suggest that methanogens take longer than 4 weeks to adapt to dietary changes and call into question the validity of experimental results based upon a 2- to 4-week acclimatization period normally observed for bacteria.           </p>", "keywords": ["Rumen", "Molecular Sequence Data", "DNA", " Ribosomal", "630", "Antibodies", "Plasma", "RNA", " Ribosomal", " 16S", "2402 Applied Microbiology and Biotechnology", "Animals", "Saliva", "1106 Food Science", "2. Zero hunger", "Vaccines", "Gastric Juice", "Sheep", "0402 animal and dairy science", "Biodiversity", "Sequence Analysis", " DNA", "04 agricultural and veterinary sciences", "Archaea", "3. Good health", "DNA", " Archaeal", "Immunoglobulin G", "1305 Biotechnology", "2303 Ecology", "Methane"]}, "links": [{"href": "https://doi.org/10.1128/aem.02453-08"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20and%20Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/aem.02453-08", "name": "item", "description": "10.1128/aem.02453-08", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/aem.02453-08"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-04-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Applied+Microbiology+and+Biotechnology&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Applied+Microbiology+and+Biotechnology&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Applied+Microbiology+and+Biotechnology&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Applied+Microbiology+and+Biotechnology&offset=6", "hreflang": "en-US"}], "numberMatched": 6, "numberReturned": 6, "distributedFeatures": [], "timeStamp": "2026-07-28T04:10:44.032521Z"}