Moss-associated nitrogen (N2) fixation is one of the main inputs of new N in pristine ecosystems that receive low amounts of atmospheric N deposition. Previous studies have shown that N2 fixation is inhibited by inorganic N (IN) inputs, but if N2 fixation in mosses is similarly affected by organic N (ON) remains unknown. Here, we assessed N2 fixation in two dominant mosses in boreal forests (
A positive soil carbon (C)\uffe2\uff80\uff90climate feedback is embedded into the climatic models of the IPCC. However, recent global syntheses indicate that the temperature sensitivity of soil respiration (RS) in drylands, the largest biome on Earth, is actually lower in warmed than in control plots. Consequently, soil C losses with future warming are expected to be low compared with other biomes. Nevertheless, the empirical basis for these global extrapolations is still poor in drylands, due to the low number of field experiments testing the pathways behind the long\uffe2\uff80\uff90term responses of soil respiration (RS) to warming. Importantly, global drylands are covered with biocrusts (communities formed by bryophytes, lichens, cyanobacteria, fungi, and bacteria), and thus,RSresponses to warming may be driven by both autotrophic and heterotrophic pathways. Here, we evaluated the effects of 8\uffe2\uff80\uff90year experimental warming onRS, and the different pathways involved, in a biocrust\uffe2\uff80\uff90dominated dryland in southern Spain. We also assessed the overall impacts on soil organic C (SOC) accumulation over time. Across the years and biocrust cover levels, warming reducedRSby 0.30\uffc2\uffa0\uffce\uffbcmol\uffc2\uffa0CO2\uffc2\uffa0m\uffe2\uff88\uff922\uffc2\uffa0s\uffe2\uff88\uff921(95% CI\uffc2\uffa0=\uffc2\uffa0\uffe2\uff88\uff920.24 to 0.84), although the negative warming effects were only significant after 3\uffc2\uffa0years of elevated temperatures in areas with low initial biocrust cover. We found support for different pathways regulating the warming\uffe2\uff80\uff90induced reduction inRSat areas with low (microbial thermal acclimation via reduced soil mass\uffe2\uff80\uff90specific respiration and \uffce\uffb2\uffe2\uff80\uff90glucosidase enzymatic activity) vs. high (microbial thermal acclimation jointly with a reduction in autotrophic respiration from decreased lichen cover) initial biocrust cover. Our 8\uffe2\uff80\uff90year experimental study shows a reduction in soil respiration with warming and highlights that biocrusts should be explicitly included in modeling efforts aimed to quantify the soil C\uffe2\uff80\uff93climate feedback in drylands.Increasing food demand coupled with climate change pose a great challenge to agricultural systems. In this review we summarize recent advances in our knowledge of how plants, together with their associated microbiota, shape rhizosphere processes. We address (molecular) mechanisms operating at the plant\uffe2\uff80\uff93microbe-soil interface and aim to link this knowledge with actual and potential avenues for intensifying agricultural systems, while at the same time reducing irrigation water, fertilizer inputs and pesticide use. Combining in-depth knowledge about above and belowground plant traits will not only significantly advance our mechanistic understanding of involved processes but also allow for more informed decisions regarding agricultural practices and plant breeding. Including belowground plant-soil-microbe interactions in our breeding efforts will help to select crops resilient to abiotic and biotic environmental stresses and ultimately enable us to produce sufficient food in a more sustainable agriculture in the upcoming decades.Vitaceae) with amplicon sequence data structures", "description": "Abstract
Impacts of plant genotype on microbial assemblage in the phyllosphere (above-ground parts of plants, which predominantly consists of the set of photosynthetic leaves) of Vitis vinifera cultivars have been studied previously but the impact of grape species (under the grape family Vitaceae) was never investigated. Considering the fact, that the phyllosphere microbiome may have profound effects on host plant health and its performance traits, studying the impact of grape species in microbial taxa structuring in the phyllosphere could be of crucial importance. We performed 16S and ITS profiling (for bacteria and fungi respectively) to access genus level characterization of the microflora present in the leaf phyllosphere of five species within this plant family, sampled in two successive years from the repository situated in the Mediterranean. We also performed \uffce\uffb1 and \uffce\uffb2-diversity analyses with robust statistical estimates to test the impacts of grape species and growing year, over a two-year period. Our results indicated the presence of complex microbial diversity and assemblages in the phyllosphere with a significant effect of both factors (grape species and growing year), the latter effect is being more pronounced. We also compared separate normalization methods for high-throughput microbiome data-sets followed by differential taxa abundance analyses. The results suggested the predominance of a particular normalization method over others. This also indicated the need for more robust normalization methods to study the differential taxa abundance among groups in microbiome research.
", "keywords": ["580", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Vegetal Biology", "Bacteria", "Microbiota", "Fungi", "microbiome", "15. Life on land", "Article", "Plant Leaves", "03 medical and health sciences", "vitis vinifera", "diversit\u00e9 microbienne", "phyllosphere", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "effet g\u00e9notype", "Vitis", "Biologie v\u00e9g\u00e9tale", "Phylogeny"]}, "links": [{"href": "https://www.nature.com/articles/s41598-019-50839-0.pdf"}, {"href": "https://hal.inrae.fr/hal-02617774/file/2019_Singh_Scientific%20Reports_1.pdf"}, {"href": "https://doi.org/10.1038/s41598-019-50839-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41598-019-50839-0", "name": "item", "description": "10.1038/s41598-019-50839-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41598-019-50839-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-10-04T00:00:00Z"}}, {"id": "10.1016/j.biortech.2006.11.027", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-01T16:15:49Z", "type": "Journal Article", "created": "2007-01-06", "title": "Effect Of Long-Term Application Of Manure And Fertilizer On Biological And Biochemical Activities In Soil During Crop Development Stages", "description": "A field experiment was conducted to investigate the effect of six long-term (34-year) fertilizer and farmyard manure (FYM) treatments (Control, N, NP, NPK, NPK+S, NPK+FYM) and three physiological stages of wheat growth on the microbial biomass carbon (MBC), nitrogen (MBN) and dehydrogenase, mineralizable N and phosphatase activities in soil. It was found that a balanced application of NPK+FYM gave the highest values for the measured parameters and lowest at the control. Values were generally highest at tillering, followed by the flowering and dough stages. A significant positive interaction between fertilizer treatments and physiological stages of wheat growth was observed, being highest at maximum tillering due to application of NPK+FYM. Stepwise regressions have revealed that grain yield of wheat was significantly associated with mineralizable N at tillering (R(2)=0.80), MBC at flowering (R(2)=0.90) and alkaline phosphatase activity (R(2)=0.70) at dough stages of wheat growth.", "keywords": ["Crops", " Agricultural", "2. Zero hunger", "Time Factors", "Bacteria", "Nitrogen", "Fungi", "04 agricultural and veterinary sciences", "Carbon", "Phosphoric Monoester Hydrolases", "Manure", "Soil", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Fertilizers", "Oxidoreductases", "Soil Microbiology", "Triticum"]}, "links": [{"href": "https://doi.org/10.1016/j.biortech.2006.11.027"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biortech.2006.11.027", "name": "item", "description": "10.1016/j.biortech.2006.11.027", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biortech.2006.11.027"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-12-01T00:00:00Z"}}, {"id": "10.1016/j.bioelechem.2020.107621", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-01T16:15:47Z", "type": "Journal Article", "created": "2020-07-27", "title": "Tailored glycosylated anode surfaces: Addressing the exoelectrogen bacterial community via functional layers for microbial fuel cell applications", "description": "Grafting of aryldiazonium cations bearing a p-mannoside functionality over microbial fuel cell (MFC) anode materials was performed to investigate the ability of aryl-glycoside layers to regulate colonisation by biocatalytic biofilms. Covalent attachment was achieved via spontaneous reactions and via electrochemically-assisted grafting using potential step experiments. The effect of different functionalisation protocols on MFC performance is discussed in terms of changes in wettability, roughness and electrochemical response of modified electrodes. Water contact angle measurements (WCA) show that aryl-mannoside grafting yields a significant increase in hydrophilic character. Surface roughness determinations via atomic force microscopy (AFM) suggest a more disordered glycan adlayer when electrografting is used to facilitate chemisorption. MFCs were used as living sensors to successfully test the coated electrodes: the response of the MFCs in terms of start-up time was accelerated when compared to that of MFC equipped with non-modified electrodes, this suggests a faster development of a mature biofilm community resulting from aryldiazonium modifications, as confirmed by cyclic voltammetry of MFC anodes. These results therefore indicate that modification with glycans offers a bioinspired route to accelerating biofilm colonisation without any adverse effects on final MFC outputs.", "keywords": ["Microbial fuel cell", "Glycosylation", "Bacteria", "Bioelectric Energy Sources", "Surface Properties", "Microbiota", "02 engineering and technology", "Exoelectrogen biofilms", "540", "7. Clean energy", "01 natural sciences", "[SDV.BIO] Life Sciences [q-bio]/Biotechnology", "0104 chemical sciences", "Electricity", "[CHIM.OTHE] Chemical Sciences/Other", "Biofilms", "Aryl-mannoside layers", "Glycan adlayers", "0210 nano-technology", "Electrodes"]}, "links": [{"href": "https://doi.org/10.1016/j.bioelechem.2020.107621"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioelectrochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.bioelechem.2020.107621", "name": "item", "description": "10.1016/j.bioelechem.2020.107621", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.bioelechem.2020.107621"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-01T00:00:00Z"}}, {"id": "10.1890/12-1243.1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-01T16:19:56Z", "type": "Journal Article", "created": "2012-10-29", "title": "Microbial Abundance And Composition Influence Litter Decomposition Response To Environmental Change", "description": "Rates of ecosystem processes such as decomposition are likely to change as a result of human impacts on the environment. In southern California, climate change and nitrogen (N) deposition in particular may alter biological communities and ecosystem processes. These drivers may affect decomposition directly, through changes in abiotic conditions, and indirectly through changes in plant and decomposer communities. To assess indirect effects on litter decomposition, we reciprocally transplanted microbial communities and plant litter among control and treatment plots (either drought or N addition) in a grassland ecosystem. We hypothesized that drought would reduce decomposition rates through moisture limitation of decomposers and reductions in plant litter quality before and during decomposition. In contrast, we predicted that N deposition would stimulate decomposition by relieving N limitation of decomposers and improving plant litter quality. We also hypothesized that adaptive mechanisms would allow microbes to decompose litter more effectively in their native plot and litter environments. Consistent with our first hypothesis, we found that drought treatment reduced litter mass loss from 20.9% to 15.3% after six months. There was a similar decline in mass loss of litter inoculated with microbes transplanted from the drought treatment, suggesting a legacy effect of drought driven by declines in microbial abundance and possible changes in microbial community composition. Bacterial cell densities were up to 86% lower in drought plots and at least 50% lower on litter derived from the drought treatment, whereas fungal hyphal lengths increased by 13\uffe2\uff80\uff9314% in the drought treatment. Nitrogen effects on decomposition rates and microbial abundances were weaker than drought effects, although N addition significantly altered initial plant litter chemistry and litter chemistry during decomposition. However, we did find support for microbial adaptation to N addition with N\uffe2\uff80\uff90derived microbes facilitating greater mass loss in N plots than in control plots. Our results show that environmental changes can affect rates of ecosystem processes directly through abiotic changes and indirectly through microbial abundances and communities. Therefore models of ecosystem response to global change may need to represent microbial biomass and community composition to make accurate predictions.
", "keywords": ["Time Factors", "Nitrogen", "Precipitation", "Nitrogen fertilization", "Environmental Microbiology", "Community composition", "Animals", "Home field advantage", "Global change", "Ecosystem", "2. Zero hunger", "Drought", "Bacteria", "Litter decomposition", "Fungi", "04 agricultural and veterinary sciences", "15. Life on land", "Grassland", "Reciprocal transplant", "6. Clean water", "Droughts", "Plant Leaves", "Microbes", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Environmental Monitoring"]}, "links": [{"href": "https://escholarship.org/content/qt5bg595vm/qt5bg595vm.pdf"}, {"href": "https://doi.org/10.1890/12-1243.1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1890/12-1243.1", "name": "item", "description": "10.1890/12-1243.1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1890/12-1243.1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-03-01T00:00:00Z"}}, {"id": "10.1021/acs.est.1c03586", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-01T16:17:21Z", "type": "Journal Article", "created": "2021-11-02", "title": "Impact of Periodic Polarization on Groundwater Denitrification in Bioelectrochemical Systems.", "description": "Nitrate contamination is a common problem in groundwater around the world. Nitrate can be cathodically reduced in bioelectrochemical systems using autotrophic denitrifiers with low energy investment and without chemical addition. Successful denitrification was demonstrated in previous studies in both microbial fuel cells and microbial electrolysis cells (MECs) with continuous current flow, whereas the impact of intermittent current supply (e.g., in a fluidized-bed system) on denitrification and particularly the electron-storing capacity of the denitrifying electroactive biofilms (EABs) on the cathodes have not been studied in depth. In this study, two continuously fed MECs were operated in parallel under continuous and periodic polarization modes over 280 days, respectively. Under continuous polarization, the maximum denitrification rate reached 233 g NO3--N/m3/d with 98% nitrate removal (0.6 mg NO3--N/L in the effluent) with negligible intermediate production, while under a 30 s open-circuit/30 s polarization mode, 86% of nitrate was removed at a maximum rate of 205 g NO3--N/m3/d (4.5 mg NO3--N/L in the effluent) with higher N2O production (6.6-9.3 mg N/L in the effluent). Conversely, periodic polarization could be an interesting approach in other bioelectrochemical processes if the generation of chemical intermediates (partially reduced or oxidized) should be favored. Similar microbial communities dominated byGallionellaceaewere found in both MECs; however, swapping the polarization modes and the electrochemical analyses suggested that the periodically polarized EABs probably developed a higher ability for electron storage and transfer, which supported the direct electron transfer pathway in discontinuous operation or fluidized biocathodes.", "keywords": ["bioelectrochemical systems (BESs)", "periodic polarization", "Autotrophic Processes", "Technology and Engineering", "denitrification", "Nitrates", "AUTOTROPHIC DENITRIFICATION", "EABs", "Bioelectric Energy Sources", "NITRATE-CONTAMINATED GROUNDWATER", "02 engineering and technology", "6. Clean water", "REDUCTION", "REMOVAL", "13. Climate action", "Earth and Environmental Sciences", "BACTERIA", "ACETATE", "Denitrification", "MICROBIAL FUEL-CELLS", "ELECTRON-TRANSFER", "BIOFILM", "0210 nano-technology", "Groundwater", "STORAGE"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.1c03586"}, {"href": "https://doi.org/10.1021/acs.est.1c03586"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.est.1c03586", "name": "item", "description": "10.1021/acs.est.1c03586", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.est.1c03586"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-11-02T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2024.170290", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-01T16:16:49Z", "type": "Journal Article", "created": "2024-01-19", "title": "Metagenomics untangles potential adaptations of Antarctic endolithic bacteria at the fringe of habitability", "description": "Survival and growth strategies of Antarctic endolithic microbes residing in Earth's driest and coldest desert remain virtually unknown. From 109 endolithic microbiomes, 4539 metagenome-assembled genomes were generated, 49.3\u00a0% of which were novel candidate bacterial species. We present evidence that trace gas oxidation and atmospheric chemosynthesis may be the prevalent strategies supporting metabolic activity and persistence of these ecosystems at the fringe of life and the limits of habitability.", "keywords": ["570", "Bacteria", "Microbiota", "Habitability", "500", "Antarctic Regions", "Astronomical Sciences", "15. Life on land", "Extremophiles", "13. Climate action", "Physical Sciences", "Antarctica", "Metagenome", "Metagenomics", "14. Life underwater", "Adaptation", "MAGs", "Settore BIO/19 - MICROBIOLOGIA GENERALE", "Environmental Sciences"]}, "links": [{"href": "https://openpub.fmach.it/bitstream/10449/83880/5/2024%20STE%20Albanese.pdf"}, {"href": "https://doi.org/10.1016/j.scitotenv.2024.170290"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2024.170290", "name": "item", "description": "10.1016/j.scitotenv.2024.170290", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2024.170290"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-03-01T00:00:00Z"}}, {"id": "10.1016/j.syapm.2012.10.007", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-01T16:17:14Z", "type": "Journal Article", "created": "2013-01-18", "title": "Shifts In Soil Bacterial Community After Eight Years Of Land-Use Change", "description": "The interaction between plants, soil and microorganisms is considered to be the major driver of ecosystem functions and any modification of plant cover and/or soil properties might affect the microbial structure, which, in turn, will influence ecological processes. Assuming that soil properties are the major drivers of soil bacterial diversity and structure within the same soil type, it can be postulated whether plant cover causes significant shifts in soil bacterial community composition. To address this question, this study used 16S rRNA pyrosequencing to detect differences in diversity, composition and/or relative abundance of bacterial taxa from an area covered by pristine forest, as well as eight-year-old grassland surrounded by the same forest. It was shown that a total of 69% of the operational taxonomic units (OTUs) were shared between environments. Overall, forest and grassland samples presented the same diversity and the clustering analysis did not show the occurrence of very distinctive bacterial communities between environments. However, 11 OTUs were detected in statistically significant higher abundance in the forest samples but in lower abundance in the grassland samples, whereas 12 OTUs occurred in statistically significant higher abundance in the grassland samples but in lower abundance in the forest samples. The results suggested the prevalence of a resilient core microbial community that did not suffer any change related to land use, soil type or edaphic conditions. The results illustrated that the history of land use might influence present-day community structure.", "keywords": ["DNA", " Bacterial", "2. Zero hunger", "Agriculture", "Sequence Analysis", " DNA", "04 agricultural and veterinary sciences", "Plants", "15. Life on land", "Biota", "DNA", " Ribosomal", "Trees", "13. Climate action", "RNA", " Ribosomal", " 16S", "Cluster Analysis", "0401 agriculture", " forestry", " and fisheries", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.syapm.2012.10.007"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Systematic%20and%20Applied%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.syapm.2012.10.007", "name": "item", "description": "10.1016/j.syapm.2012.10.007", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.syapm.2012.10.007"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-03-01T00:00:00Z"}}, {"id": "10.1016/j.syapm.2020.126149", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-01T16:17:14Z", "type": "Journal Article", "created": "2020-09-30", "title": "Genetic diversity and phylogeny of indigenous rhizobia nodulating faba bean (Vicia faba L.) in Greece", "description": "The genetic diversity and phylogeny of fast-growing rhizobia isolated from root nodules of Vicia faba grown in different geographical regions of Greece were assessed. Although Rhizobium leguminosarum sv. viciae is the most common symbiont of Vicia spp. in European soils, there is no available information on native rhizobia nodulating faba bean in Greece. Seventy bacterial strains were isolated and grouped into sixteen distinct profiles based on BOX-PCR fingerprinting. The phylogenetic affiliation was further defined by sequence analysis of the rrs and multilocus sequence analysis (MLSA) of three housekeeping genes (recA, atpD and gyrB). Fifty-eight isolates were affiliated with recently described genospecies gsF-2, represented by R. laguerreae FB206T, whereas six isolates were closely related to gsB and two isolates might belong to gsA. Two isolates assigned to R. hidalgonense and another two non-nodulating strains could not be assigned to any validly defined species and possibly belong to a new rhizobial lineage. Interestingly, R. laguerreae strains were commonly found at all sampling sites, suggesting that they could be the main symbionts of faba beans in Greek soils. According to the phylogenies of two symbiosis-related genes (nodC and nifH), all nodulating isolates belonged to symbiovar (sv.) viciae harboring four distinct nodC gene haplotypes and they were grouped into two clades together with strains assigned to R. laguerreae and genospecies of R. leguminosarum isolated from other countries and continents. This is the first report that R. hidalgonense strains belong to sv. viciae. No correlation was observed between the nodC haplotypes, geographic origin and chromosomal background of the isolates in the study.", "keywords": ["MLSA", "DNA", " Bacterial", "0301 basic medicine", "0303 health sciences", "Genes", " Essential", "Greece", "Viciae", "Sequence Analysis", " DNA", "DNA Fingerprinting", "Vicia faba", "03 medical and health sciences", "Symbiovar", "Genes", " Bacterial", "RNA", " Ribosomal", " 16S", "Root Nodules", " Plant", "Symbiosis", "Phylogeny", "Soil Microbiology", "Multilocus Sequence Typing", "Rhizobium"]}, "links": [{"href": "https://doi.org/10.1016/j.syapm.2020.126149"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Systematic%20and%20Applied%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.syapm.2020.126149", "name": "item", "description": "10.1016/j.syapm.2020.126149", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.syapm.2020.126149"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-11-01T00:00:00Z"}}, {"id": "10.1016/j.tcsw.2021.100059", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-01T16:17:14Z", "type": "Journal Article", "created": "2021-07-16", "title": "High-resolution 3D mapping of rhizosphere glycan patterning using molecular probes in a transparent soil system", "description": "Rhizospheres are microecological zones at the interface of roots and soils. Interactions between bacteria and roots are critical for maintaining plant and soil health but are difficult to study because of constraints inherent in working with underground systems. We have developed an in-situ rhizosphere imaging system based on transparent soils and molecular probes that can be imaged using confocal microscopy. We observed spatial patterning of polysaccharides along roots and on cells deposited into the rhizosphere and also co-localised fluorescently tagged soil bacteria. These studies provide insight into the complex glycan landscape of rhizospheres and suggest a means by which root / rhizobacteria interactions can be non-disruptively studied.", "keywords": ["0301 basic medicine", "570", "0303 health sciences", "QH573-671", "15. Life on land", "630", "Article", "Transparent Soil", "03 medical and health sciences", "Fluorescence Microscopy", "Rhizosphere", "Rhizobacteria", "Polysaccharide", "Cytology"]}, "links": [{"href": "https://eprints.ncl.ac.uk/fulltext.aspx?url=279778/5D67D23B-DAA5-4CF5-A60A-16112D9E3664.pdf&pub_id=279778"}, {"href": "https://doi.org/10.1016/j.tcsw.2021.100059"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20Cell%20Surface", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.tcsw.2021.100059", "name": "item", "description": "10.1016/j.tcsw.2021.100059", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.tcsw.2021.100059"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-12-01T00:00:00Z"}}, {"id": "10.1016/j.watres.2019.05.025", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-01T16:17:16Z", "type": "Journal Article", "created": "2019-05-09", "title": "Continuous ozonation of urban wastewater: Removal of antibiotics, antibiotic-resistant Escherichia coli and antibiotic resistance genes and phytotoxicity", "description": "This work evaluated the removal of a mixture of eight antibiotics (i.e. ampicillin (AMP), azithromycin (AZM), erythromycin (ERY), clarithromycin (CLA), ofloxacin (OFL), sulfamethoxazole (SMX), trimethoprim (TMP) and tetracycline (TC)) from urban wastewater, by ozonation operated in continuous mode at different hydraulic retention times (HRTs) (i.e. 10, 20, 40 and 60\u202fmin) and specific ozone doses (i.e. 0.125, 0.25, 0.50 and 0.75 gO3 gDOC- 1). As expected, the efficiency of ozonation was highly ozone dose- and contact time-dependent. The removal of the parent compounds of the selected antibiotics to levels below their detection limits was achieved with HRT of 40\u202fmin and specific ozone dose of 0.125 gO3 gDOC- 1. The effect of ozonation was also investigated at a microbiological and genomic level, by studying the efficiency of the process with respect to the inactivation of Escherichia coli and antibiotic-resistant E.\u00a0coli, as well as to the reduction of the abundance of selected antibiotic resistance genes (ARGs). The inactivation of total cultivable E.\u00a0coli was achieved under the experimental conditions of HRT 40\u202fmin and 0.25 gO3 gDOC-1, at which all antibiotic compounds were already degraded. The regrowth examinations revealed that higher ozone concentrations were required for the permanent inactivation of E.\u00a0coli below the Limit of Quantification (