{"type": "FeatureCollection", "features": [{"id": "10.1016/j.fm.2020.103583", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-02T16:16:26Z", "type": "Journal Article", "created": "2020-06-27", "title": "Combined application of antagonistic Wickerhamomyces anomalus BS91 strain and Cynara cardunculus L. leaf extracts for the control of postharvest decay of citrus fruit", "description": "Combined use of biocontrol agents and plant extracts can be considered a viable and promising strategy for protecting plant tissues with different synergistic mechanisms of action that improve the antimicrobial activity of the mixtures. Treatments of citrus fruits with Wickerhamomyces anomalus BS91 have been previously reported as effective measures to reduce the incidence of green mold caused by Penicillium digitatum. On the opposite, the knowledge of the antifungal activity of cultivated cardoon (Cynara cardunculus L. var. altilis DC.) leaf extract, vegetable widespread in some Mediterranean areas, is still very limited. In this study, experimental trials were conducted to evaluate the effectiveness in vitro of leaf aqueous, methanolic and ethanolic extracts of C. cardunculus against seven fungal pathogens responsible for considerable food losses in the postharvest stage. In addition, biocontrol yeast W. anomalus BS91 and the three C. cardunculus extracts were tested in vivo both as a single treatment and in mixture, against Penicillium digitatum on 'Tarocco' oranges and 'Femminello' lemons. The combination of W. anomalus BS91 and leaf ethanolic extract reduced with the highest efficacy the incidence and severity of green mold on orange and lemon fruits with respect to the control, and was more effective than treatment with antagonistic yeast or leaf extracts applied alone. Incidence and severity of citrus decay were more consistently reduced when mixtures were applied 24\u00a0h before the inoculation of the pathogen, thus suggesting the relevance of preventive treatments. The mixtures of antagonistic W. anomalus BS91 and ethanolic leaf extract were more effective in controlling green mold decay on oranges than on lemons. These results indicate that biocontrol agents and leaf extracts, used in appropriate combination, can provide a stronger protection than when used singularly. However, compatibility between microbial antagonist and antimicrobial extract should be preliminary verified.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Citrus", "0303 health sciences", "Plant Extracts", "Penicillium", "Biocontrol; Yeasts; Cynara cardunculus; Citrus; Green mold; Postharvest", "Cynara", "Biocontrol", " Citrus", " Cynara cardunculus", " Green mold", " Postharvest", " Yeasts", "03 medical and health sciences", "Biological Control Agents", "Food Preservation", "Fruit", "Antibiosis", "Saccharomycetales", "Biocontrol; Citrus; Cynara cardunculus; Green mold; Postharvest; Yeasts", "Plant Diseases"]}, "links": [{"href": "https://www.iris.unict.it/bitstream/20.500.11769/494574/1/Food%20Microbiology%202020.pdf"}, {"href": "https://doi.org/10.1016/j.fm.2020.103583"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Food%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.fm.2020.103583", "name": "item", "description": "10.1016/j.fm.2020.103583", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.fm.2020.103583"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-01T00:00:00Z"}}, {"id": "10.1038/ismej.2016.169", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-02T16:17:54Z", "type": "Journal Article", "created": "2017-01-03", "title": "The Pseudomonas putida T6SS is a plant warden against phytopathogens", "description": "Abstract                <p>Bacterial type VI secretion systems (T6SSs) are molecular weapons designed to deliver toxic effectors into prey cells. These nanomachines have an important role in inter-bacterial competition and provide advantages to T6SS active strains in polymicrobial environments. Here we analyze the genome of the biocontrol agent Pseudomonas putida KT2440 and identify three T6SS gene clusters (K1-, K2- and K3-T6SS). Besides, 10 T6SS effector\uffe2\uff80\uff93immunity pairs were found, including putative nucleases and pore-forming colicins. We show that the K1-T6SS is a potent antibacterial device, which secretes a toxic Rhs-type effector Tke2. Remarkably, P. putida eradicates a broad range of bacteria in a K1-T6SS-dependent manner, including resilient phytopathogens, which demonstrates that the T6SS is instrumental to empower P. putida to fight against competitors. Furthermore, we observed a drastically reduced necrosis on the leaves of Nicotiana benthamiana during co-infection with P. putida and Xanthomonas campestris. Such protection is dependent on the activity of the P. putida T6SS. Many routes have been explored to develop biocontrol agents capable of manipulating the microbial composition of the rhizosphere and phyllosphere. Here we unveil a novel mechanism for plant biocontrol, which needs to be considered for the selection of plant wardens whose mission is to prevent phytopathogen infections.</p>", "keywords": ["PROTEIN SECRETION", "Nicotiana", "0301 basic medicine", "570", "INTESTINAL INFLAMMATION", "05 Environmental Sciences", "VIBRIO-CHOLERAE", "Environmental Sciences & Ecology", "VI SECRETION SYSTEM", "Xanthomonas campestris", "Microbiology", "03 medical and health sciences", "Bacterial Proteins", "10 Technology", "Plant Diseases", "0303 health sciences", "Science & Technology", "Ecology", "Pseudomonas putida", "ROOT MICROBIOME", "Gene Expression Regulation", " Bacterial", "06 Biological Sciences", "Type VI Secretion Systems", "GENOMIC ANALYSIS", "Biological Control Agents", "ESCHERICHIA-COLI", "EFFECTORS", "IMMUNITY PROTEINS", "Original Article", "HOST-RANGE", "Life Sciences & Biomedicine"]}, "links": [{"href": "http://www.nature.com/articles/ismej2016169.pdf"}, {"href": "https://doi.org/10.1038/ismej.2016.169"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20ISME%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/ismej.2016.169", "name": "item", "description": "10.1038/ismej.2016.169", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/ismej.2016.169"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-03T00:00:00Z"}}, {"id": "10.1038/s41467-020-15586-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-02T16:17:56Z", "type": "Journal Article", "created": "2020-04-21", "title": "Biological weed control to relieve millions from Ambrosia allergies in Europe", "description": "Abstract<p>Invasive alien species (IAS) can substantially affect ecosystem services and human well-being. However, quantitative assessments of their impact on human health are rare and the benefits of implementing IAS management likely to be underestimated. Here we report the effects of the allergenic plant Ambrosia artemisiifolia on public health in Europe and the potential impact of the accidentally introduced leaf beetle Ophraella communa on the number of patients and healthcare costs. We find that, prior to the establishment of O. communa, some 13.5 million persons suffered from Ambrosia-induced allergies in Europe, causing costs of Euro 7.4 billion annually. Our projections reveal that biological control of A. artemisiifolia will reduce the number of patients by approximately 2.3 million and the health costs by Euro 1.1 billion per year. Our conservative calculations indicate that the currently discussed economic costs of IAS underestimate the real costs and thus also the benefits from biological control.</p>", "keywords": ["0106 biological sciences", "2. Zero hunger", "Science", "Weed Control", "Q", "Plant Weeds", "Rhinitis", " Allergic", " Seasonal", "15. Life on land", "Q1", "01 natural sciences", "Article", "3. Good health", "Coleoptera", "Europe", "Biological Control Agents", "13. Climate action", "Animals", "Humans", "Public Health", "Ambrosia"]}, "links": [{"href": "https://eprints.worc.ac.uk/9268/15/Skj%C3%B8th-9268-VoR-Biological-Weed-Control-to-relieve-millions-from-Ambrosia-allergies-in-Europe.pdf"}, {"href": "https://www.nature.com/articles/s41467-020-15586-1.pdf"}, {"href": "https://doi.org/10.1038/s41467-020-15586-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-020-15586-1", "name": "item", "description": "10.1038/s41467-020-15586-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-020-15586-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-21T00:00:00Z"}}, {"id": "10.1094/PHYTO-07-17-0255-R", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-02T16:18:38Z", "type": "Journal Article", "created": "2018-01-31", "title": "Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize", "description": "<p>Maize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the present study, 83 A. flavus isolates from two maize regions of Argentina were characterized and evaluated for their ability to produce or lack of producing mycotoxins in order to select atoxigenic strains to be used as potential biocontrol agents (BCA). All of the isolates were tested for aflatoxin and cyclopiazonic acid (CPA) production in maize kernels and a liquid culture medium. Genetic diversity of the nonaflatoxigenic isolates was evaluated by analysis of vegetative compatibility groups (VCG) and confirmation of deletions in the aflatoxin biosynthesis cluster. Eight atoxigenic isolates were compared for their ability to reduce aflatoxin and CPA contamination in maize kernels in coinoculation tests. The A. flavus population was composed of 32% aflatoxin and CPA producers and 52% CPA producers, and 16% was determined as atoxigenic. All of the aflatoxin producer isolates also produced CPA. Aflatoxin and CPA production was significantly higher in maize kernels than in liquid medium. The 57 nonaflatoxigenic strains formed six VCG, with AM1 and AM5 being the dominant groups, with a frequency of 58 and 35%, respectively. In coinoculation experiments, all of the atoxigenic strains reduced aflatoxin from 54 to 83% and CPA from 60 to 97%. Members of group AM1 showed a greater aflatoxin reduction than members of AM5 (72 versus 66%) but no differences were detected in CPA production. Here, we described for the first time atoxigenic isolates of A. flavus that show promise to be used as BCA in maize crops in Argentina. This innovating biological control approach should be considered, developed further, and used by the maize industry to preserve the quality properties and food safety of maize kernels in Argentina.</p>", "keywords": ["0301 basic medicine", "2. Zero hunger", "Micotoxinas", "0303 health sciences", "Argentina", "Biocontrol", "Genetic Variation", "Mycotoxins", "maize", "Zea mays", "Maize", "03 medical and health sciences", "https://purl.org/becyt/ford/4.5", "Biological Control Agents", "13. Climate action", "Aflatoxinas", "Antibiosis", "https://purl.org/becyt/ford/4", "Aspergillus Flavus", "Zea Mays", "Ma\u00edz", "Aspergillus flavus", "Plant Diseases"]}, "links": [{"href": "https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-07-17-0255-R"}, {"href": "https://doi.org/10.1094/PHYTO-07-17-0255-R"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Phytopathology%C2%AE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1094/PHYTO-07-17-0255-R", "name": "item", "description": "10.1094/PHYTO-07-17-0255-R", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1094/PHYTO-07-17-0255-R"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-01T00:00:00Z"}}, {"id": "10.1094/phyto-07-17-0255-r", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-02T16:18:39Z", "type": "Journal Article", "created": "2018-01-31", "title": "Characterization of Argentinian EndemicAspergillus flavusIsolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Maize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the present study, 83 A. flavus isolates from two maize regions of Argentina were characterized and evaluated for their ability to produce or lack of producing mycotoxins in order to select atoxigenic strains to be used as potential biocontrol agents (BCA). All of the isolates were tested for aflatoxin and cyclopiazonic acid (CPA) production in maize kernels and a liquid culture medium. Genetic diversity of the nonaflatoxigenic isolates was evaluated by analysis of vegetative compatibility groups (VCG) and confirmation of deletions in the aflatoxin biosynthesis cluster. Eight atoxigenic isolates were compared for their ability to reduce aflatoxin and CPA contamination in maize kernels in coinoculation tests. The A. flavus population was composed of 32% aflatoxin and CPA producers and 52% CPA producers, and 16% was determined as atoxigenic. All of the aflatoxin producer isolates also produced CPA. Aflatoxin and CPA production was significantly higher in maize kernels than in liquid medium. The 57 nonaflatoxigenic strains formed six VCG, with AM1 and AM5 being the dominant groups, with a frequency of 58 and 35%, respectively. In coinoculation experiments, all of the atoxigenic strains reduced aflatoxin from 54 to 83% and CPA from 60 to 97%. Members of group AM1 showed a greater aflatoxin reduction than members of AM5 (72 versus 66%) but no differences were detected in CPA production. Here, we described for the first time atoxigenic isolates of A. flavus that show promise to be used as BCA in maize crops in Argentina. This innovating biological control approach should be considered, developed further, and used by the maize industry to preserve the quality properties and food safety of maize kernels in Argentina.</p></article>", "keywords": ["0301 basic medicine", "2. Zero hunger", "Micotoxinas", "0303 health sciences", "Argentina", "Biocontrol", "Genetic Variation", "Mycotoxins", "maize", "Zea mays", "Maize", "03 medical and health sciences", "https://purl.org/becyt/ford/4.5", "Biological Control Agents", "13. Climate action", "Aflatoxinas", "Antibiosis", "https://purl.org/becyt/ford/4", "Aspergillus Flavus", "Zea Mays", "Ma\u00edz", "Aspergillus flavus", "Plant Diseases"]}, "links": [{"href": "https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-07-17-0255-R"}, {"href": "https://doi.org/10.1094/phyto-07-17-0255-r"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Phytopathology%C2%AE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1094/phyto-07-17-0255-r", "name": "item", "description": "10.1094/phyto-07-17-0255-r", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1094/phyto-07-17-0255-r"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-01T00:00:00Z"}}, {"id": "10.1371/journal.pone.0213273", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-02T16:19:44Z", "type": "Journal Article", "created": "2019-03-26", "title": "Epicoccum layuense a potential biological control agent of esca-associated fungi in grapevine", "description": "Epicoccum is a genus of ascomycetes often associated with the mycobiome of grapevines (Vitis vinifera). Epicoccum spp. are found in the soil, phyllosphere, as well as in the wood, where they interact both with the plant and with other endophytes and pathogens. Wood pathogens involved in the esca disease complex, a grapevine trunk disease, are particularly concerning in viticulture, as current control strategies have proven unsatisfactory. This study investigated the interaction among Epicoccum spp. and three esca-associated fungi, with the aim of establishing whether they are suitable candidates for biological control.A screening conducted in vitro, by means of dual culture, revealed that all tested Epicoccum spp. inhibited the growth of pathogens Phaeomoniella chlamydospora and Fomitiporia mediterranea, while only some of them inhibited Phaeoacremonium minimum. Epicoccum layuense E24, identified as the most efficient antagonist, was tested in rooted grapevine cuttings of cultivars Cabernet Sauvignon and Touriga Nacional, under greenhouse conditions, against P. chlamydospora and P. minimum. This study revealed that the inoculation of E. layuense E24 produced a successful colonization of the wood of grapevines; in addition it did not impair the growth of the plants or induce the appearance of symptoms in leaves or in wood. Moreover, grapevines colonized by E. layuense E24 showed a considerable decrease in the wood symptomatology caused by the inoculated pathogens (by 31-82%, depending on the pathogen/grapevine cultivar), as well as a reduction in their frequency of re-isolation (60-74%).Our findings suggest that E. layuense E24 is a promising candidate for its application in biological control, due to its antagonistic interaction with some esca-associated fungal pathogens.", "keywords": ["0106 biological sciences", "2. Zero hunger", "control agent", "Science", "Q", "R", "Wood", "01 natural sciences", "grapevine", "Epicoccum layuense", "Ascomycota", "Biological Control Agents", "esca-associated fungi", "Medicine", "Vitis", "Research Article", "Plant Diseases"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0213273"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0213273", "name": "item", "description": "10.1371/journal.pone.0213273", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0213273"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-03-26T00:00:00Z"}}, {"id": "10.18174/121542", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-02T16:20:06Z", "type": "Report", "created": "2024-10-21", "title": "Integrating biological control and botanical pesticides for management of Plutella xylostella", "description": "A large number of different plant species have natural pesticidal properties, and man has made use of this since early times. By applying plant extracts to other susceptible plant species the defence of the susceptible plant is improved. This thesisfocuseson the possibility of integrating botanical pesticides with biological control for management of the diamondback moth, Plutella xylostella , inSouth Africa Twenty-one species of primary parasitoids have been collected from P. xylostella in the field inSouth Africa. Biological control therefore provides a natural control technique. However, biological control alone is insufficient to provide adequate protection and requires integration with other control techniques. Plant products from the Meliaceae family have been widely used to control insect pests, particularly products from the neem tree, Azadirachta indica . The neem tree does not grow inSouth Africabut the closely related syringa tree Melia azedarach is a widespread invasive plant found throughout the country. In this thesis I make use of a commercial neem product, Neemix 4.5 \u00ae , and aqueous leaf extracts derived from the syringa tree.The neem- and syringa- derived botanical pesticides had adverse effects on the development, reproduction and survival of P. xylostella . These botanical pesticides also reduced feeding and oviposition, which are important factors in pest control. However, if a botanical pesticide is to be combined with biological control it must not hamper natural enemies. The neem- and syringa- derived botanical pesticides did not have a directly negative impact on the survival of Cotesia plutellae or Diadromus collaris two of the most abundant natural enemies found in South Africa. In a glasshouse, a significantly higher proportion of P. xylostella larvae were parasitised by C. plutellae on plants that been treated with the syringa extract than on control plants. Results from a choice test in a windtunnel showed that C. plutellae was attracted significantly more often to cabbage plants treated with the syringa extract than to the control plants. Headspace analysis revealed that treatment of cabbage with syringa extracts caused an increased emission of volatiles by the cabbage plants. This may explain the increased attraction of C. plutellae to plants that had been treated with the syringa extract. It was important to verify results from the laboratory under more realistic conditions in the field. We did not find a difference in P. xylostella infestation levels between the treated and the control plants in the field. However, the damage on plants treated with the botanical pesticides was significantly lower. Therefore, it seems that reduced feeding by P. xylostella larvae was a more important factor in the reduction of damage than the actual population density. The proportion of P. xylostella larvae that had been parasitised was significantly higher on the treated plants than on the control plants. Direct observations showed that plants that had been treated with neem- and syringa- derived pesticides were still visited by parasitoids. Therefore these botanical pesticides do not appear to interfere with parasitoid foraging. I assessed the possibility for introducing this control method to the rural farming community inSouth Africa. Syringa trees are invasive plants found throughoutSouth Africaand therefore provide a free local resource for the botanical pesticide. Results indicated that the use of syringa extracts could be introduced. Water is the main factor limiting the introduction of this technique in more arid environments. Results presented in this thesis indicate that biological control and the use of botanical pesticides derived from the neem and syringa trees can be integrated for the management ofP. xylostella. However, mammalian toxicity and residual effects still require extensive investigation before any further recommendations can be made.", "keywords": ["insect pests", "biological control agents", "botanical insecticides", "plutella xylostella", "biological control", "melia azedarach", "parasitoids", "azadirachta indica"], "contacts": [{"organization": "Charleston, D.S.", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.18174/121542"}, {"rel": "self", "type": "application/geo+json", "title": "10.18174/121542", "name": "item", "description": "10.18174/121542", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.18174/121542"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-10-21T00:00:00Z"}}, {"id": "10.3390/genes10080601", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-02T16:21:07Z", "type": "Journal Article", "created": "2019-08-09", "title": "Genetic Potential of the Biocontrol Agent Pseudomonas brassicacearum (Formerly P. trivialis) 3Re2-7 Unraveled by Genome Sequencing and Mining, Comparative Genomics and Transcriptomics", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>The genus Pseudomonas comprises many known plant-associated microbes with plant growth promotion and disease suppression properties. Genome-based studies allow the prediction of the underlying mechanisms using genome mining tools and the analysis of the genes unique for a strain by implementing comparative genomics. Here, we provide the genome sequence of the strain Pseudomonas brassicacearum 3Re2-7, formerly known as P. trivialis and P. reactans, elucidate its revised taxonomic classification, experimentally verify the gene predictions by transcriptome sequencing, describe its genetic biocontrol potential and contextualize it to other known Pseudomonas biocontrol agents. The P. brassicacearum 3Re2-7 genome comprises a circular chromosome with a size of 6,738,544 bp and a GC-content of 60.83%. 6267 genes were annotated, of which 6113 were shown to be transcribed in rich medium and/or in the presence of Rhizoctonia solani. Genome mining identified genes related to biocontrol traits such as secondary metabolite and siderophore biosynthesis, plant growth promotion, inorganic phosphate solubilization, biosynthesis of lipo- and exopolysaccharides, exoproteases, volatiles and detoxification. Core genome analysis revealed, that the 3Re2-7 genome exhibits a high collinearity with the representative genome for the species, P. brassicacearum subsp. brassicacearum NFM421. Comparative genomics allowed the identification of 105 specific genes and revealed gene clusters that might encode specialized biocontrol mechanisms of strain 3Re2-7. Moreover, we captured the transcriptome of P. brassicacearum 3Re2-7, confirming the transcription of the predicted biocontrol-related genes. The gene clusters coding for 2,4-diacetylphloroglucinol (phlABCDEFGH) and hydrogen cyanide (hcnABC) were shown to be highly transcribed. Further genes predicted to encode putative alginate production enzymes, a pyrroloquinoline quinone precursor peptide PqqA and a matrixin family metalloprotease were also found to be highly transcribed. With this study, we provide a basis to further characterize the mechanisms for biocontrol in Pseudomonas species, towards a sustainable and safe application of P. brassicacearum biocontrol agents.</p></article>", "keywords": ["COMPARATIVE GENOMICS", "0301 basic medicine", "570", "Antifungal Agents", "Plant-growth promotion", "Biolog\u00eda", "comparative genomics", "Phloroglucinol", "PLANT-GROWTH PROMOTION", "Article", "Rhizoctonia", "12. Responsible consumption", "transcriptomics", "03 medical and health sciences", "https://purl.org/becyt/ford/1.6", "Genome mining", "Hydrogen Cyanide", "Pseudomonas", "genome mining", "RNA SEQUENCING", "TRANSCRIPTOMICS", "biocontrol", "GENOME MINING", "PSEUDOMONASBRASSICACEARUM", "https://purl.org/becyt/ford/1", "Transcriptomics", "2. Zero hunger", "0303 health sciences", "Comparative genomics", "Biocontrol", "RNA sequencing", "<i>Pseudomonas brassicacearum</i>", "3. Good health", "BIOCONTROL", "Pseudomonas brassicacearum", "Biological Control Agents", "Genes", " Bacterial", "Transcriptome", "plant-growth promotion"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/10/8/601/pdf"}, {"href": "https://www.mdpi.com/2073-4425/10/8/601/pdf"}, {"href": "https://doi.org/10.3390/genes10080601"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/genes10080601", "name": "item", "description": "10.3390/genes10080601", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/genes10080601"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-08-09T00:00:00Z"}}, {"id": "11336/151981", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-02T16:25:03Z", "type": "Journal Article", "created": "2019-08-09", "title": "Genetic Potential of the Biocontrol Agent Pseudomonas brassicacearum (Formerly P. trivialis) 3Re2-7 Unraveled by Genome Sequencing and Mining, Comparative Genomics and Transcriptomics", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>The genus Pseudomonas comprises many known plant-associated microbes with plant growth promotion and disease suppression properties. Genome-based studies allow the prediction of the underlying mechanisms using genome mining tools and the analysis of the genes unique for a strain by implementing comparative genomics. Here, we provide the genome sequence of the strain Pseudomonas brassicacearum 3Re2-7, formerly known as P. trivialis and P. reactans, elucidate its revised taxonomic classification, experimentally verify the gene predictions by transcriptome sequencing, describe its genetic biocontrol potential and contextualize it to other known Pseudomonas biocontrol agents. The P. brassicacearum 3Re2-7 genome comprises a circular chromosome with a size of 6,738,544 bp and a GC-content of 60.83%. 6267 genes were annotated, of which 6113 were shown to be transcribed in rich medium and/or in the presence of Rhizoctonia solani. Genome mining identified genes related to biocontrol traits such as secondary metabolite and siderophore biosynthesis, plant growth promotion, inorganic phosphate solubilization, biosynthesis of lipo- and exopolysaccharides, exoproteases, volatiles and detoxification. Core genome analysis revealed, that the 3Re2-7 genome exhibits a high collinearity with the representative genome for the species, P. brassicacearum subsp. brassicacearum NFM421. Comparative genomics allowed the identification of 105 specific genes and revealed gene clusters that might encode specialized biocontrol mechanisms of strain 3Re2-7. Moreover, we captured the transcriptome of P. brassicacearum 3Re2-7, confirming the transcription of the predicted biocontrol-related genes. The gene clusters coding for 2,4-diacetylphloroglucinol (phlABCDEFGH) and hydrogen cyanide (hcnABC) were shown to be highly transcribed. Further genes predicted to encode putative alginate production enzymes, a pyrroloquinoline quinone precursor peptide PqqA and a matrixin family metalloprotease were also found to be highly transcribed. With this study, we provide a basis to further characterize the mechanisms for biocontrol in Pseudomonas species, towards a sustainable and safe application of P. brassicacearum biocontrol agents.</p></article>", "keywords": ["COMPARATIVE GENOMICS", "0301 basic medicine", "570", "Antifungal Agents", "Plant-growth promotion", "Biolog\u00eda", "comparative genomics", "Phloroglucinol", "PLANT-GROWTH PROMOTION", "Article", "Rhizoctonia", "12. Responsible consumption", "transcriptomics", "03 medical and health sciences", "https://purl.org/becyt/ford/1.6", "Genome mining", "Hydrogen Cyanide", "Pseudomonas", "genome mining", "RNA SEQUENCING", "TRANSCRIPTOMICS", "biocontrol", "GENOME MINING", "PSEUDOMONASBRASSICACEARUM", "https://purl.org/becyt/ford/1", "Transcriptomics", "2. Zero hunger", "0303 health sciences", "Comparative genomics", "Biocontrol", "RNA sequencing", "<i>Pseudomonas brassicacearum</i>", "3. Good health", "BIOCONTROL", "Pseudomonas brassicacearum", "Biological Control Agents", "Genes", " Bacterial", "Transcriptome", "plant-growth promotion"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/10/8/601/pdf"}, {"href": "https://www.mdpi.com/2073-4425/10/8/601/pdf"}, {"href": "https://doi.org/11336/151981"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11336/151981", "name": "item", "description": "11336/151981", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11336/151981"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-08-09T00:00:00Z"}}, {"id": "11336/255698", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-02T16:25:03Z", "type": "Journal Article", "created": "2018-01-31", "title": "Characterization of Argentinian EndemicAspergillus flavusIsolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Maize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the present study, 83 A. flavus isolates from two maize regions of Argentina were characterized and evaluated for their ability to produce or lack of producing mycotoxins in order to select atoxigenic strains to be used as potential biocontrol agents (BCA). All of the isolates were tested for aflatoxin and cyclopiazonic acid (CPA) production in maize kernels and a liquid culture medium. Genetic diversity of the nonaflatoxigenic isolates was evaluated by analysis of vegetative compatibility groups (VCG) and confirmation of deletions in the aflatoxin biosynthesis cluster. Eight atoxigenic isolates were compared for their ability to reduce aflatoxin and CPA contamination in maize kernels in coinoculation tests. The A. flavus population was composed of 32% aflatoxin and CPA producers and 52% CPA producers, and 16% was determined as atoxigenic. All of the aflatoxin producer isolates also produced CPA. Aflatoxin and CPA production was significantly higher in maize kernels than in liquid medium. The 57 nonaflatoxigenic strains formed six VCG, with AM1 and AM5 being the dominant groups, with a frequency of 58 and 35%, respectively. In coinoculation experiments, all of the atoxigenic strains reduced aflatoxin from 54 to 83% and CPA from 60 to 97%. Members of group AM1 showed a greater aflatoxin reduction than members of AM5 (72 versus 66%) but no differences were detected in CPA production. Here, we described for the first time atoxigenic isolates of A. flavus that show promise to be used as BCA in maize crops in Argentina. This innovating biological control approach should be considered, developed further, and used by the maize industry to preserve the quality properties and food safety of maize kernels in Argentina.</p></article>", "keywords": ["2. Zero hunger", "0301 basic medicine", "Micotoxinas", "0303 health sciences", "Argentina", "Biocontrol", "Genetic Variation", "Mycotoxins", "maize", "Zea mays", "Maize", "03 medical and health sciences", "https://purl.org/becyt/ford/4.5", "Biological Control Agents", "13. Climate action", "Aflatoxinas", "Antibiosis", "https://purl.org/becyt/ford/4", "Aspergillus Flavus", "Zea Mays", "Ma\u00edz", "Aspergillus flavus", "Plant Diseases"]}, "links": [{"href": "https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-07-17-0255-R"}, {"href": "https://doi.org/11336/255698"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Phytopathology%C2%AE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11336/255698", "name": "item", "description": "11336/255698", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11336/255698"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-01T00:00:00Z"}}, {"id": "1887/3192725", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-02T16:25:20Z", "type": "Journal Article", "created": "2020-04-21", "title": "Biological weed control to relieve millions from Ambrosia allergies in Europe", "description": "Abstract<p>Invasive alien species (IAS) can substantially affect ecosystem services and human well-being. However, quantitative assessments of their impact on human health are rare and the benefits of implementing IAS management likely to be underestimated. Here we report the effects of the allergenic plant Ambrosia artemisiifolia on public health in Europe and the potential impact of the accidentally introduced leaf beetle Ophraella communa on the number of patients and healthcare costs. We find that, prior to the establishment of O. communa, some 13.5 million persons suffered from Ambrosia-induced allergies in Europe, causing costs of Euro 7.4 billion annually. Our projections reveal that biological control of A. artemisiifolia will reduce the number of patients by approximately 2.3 million and the health costs by Euro 1.1 billion per year. Our conservative calculations indicate that the currently discussed economic costs of IAS underestimate the real costs and thus also the benefits from biological control.</p", "keywords": ["2. Zero hunger", "0106 biological sciences", "Science", "Weed Control", "Q", "Plant Weeds", "Rhinitis", " Allergic", " Seasonal", "15. Life on land", "Q1", "01 natural sciences", "Article", "3. Good health", "Coleoptera", "Europe", "Biological Control Agents", "13. Climate action", "Animals", "Humans", "Public Health", "Ambrosia"]}, "links": [{"href": "https://eprints.worc.ac.uk/9268/15/Skj%C3%B8th-9268-VoR-Biological-Weed-Control-to-relieve-millions-from-Ambrosia-allergies-in-Europe.pdf"}, {"href": "https://www.nature.com/articles/s41467-020-15586-1.pdf"}, {"href": "https://doi.org/1887/3192725"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "1887/3192725", "name": "item", "description": "1887/3192725", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/1887/3192725"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-21T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Biological+Control+Agents&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Biological+Control+Agents&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Biological+Control+Agents&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Biological+Control+Agents&offset=11", "hreflang": "en-US"}], "numberMatched": 11, "numberReturned": 11, "distributedFeatures": [], "timeStamp": "2026-05-03T08:26:09.794951Z"}