{"type": "FeatureCollection", "features": [{"id": "10.1016/j.electacta.2020.137568", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:11Z", "type": "Journal Article", "created": "2020-11-30", "title": "Modelling the influence of soil properties on performance and bioremediation ability of a pile of soil microbial fuel cells", "description": "Abstract   Worldwide, intense industrial and agricultural activities pose serious issues of land contamination. Soil microbial fuel cells (SMFCs) have great potential as a low-cost, and self-powered solution to soil bioremediation, compatible with operations in remote areas. In this study, we propose a novel tubular SMFC design, in which a ceramic tube acts as the separator between the air-cathode and the anode, while providing structural support. No oxygen reduction reaction catalyst is used, and to reach depth, several SMFC units are piled together.  To assess the effect of both the system design and soil properties on performance, a mathematical model, calibrated with experimental data, is proposed, which accounts for chemical and (bio)electrochemical reactions, as well as for charge conservation and transport phenomena. The information generated provides useful indications on optimal design and operational conditions for SMFCs and a guide to effective scale-up strategies for their use in bioremediation.", "keywords": ["Soil microbial fuel cell", "/dk/atira/pure/subjectarea/asjc/1500/1500; name=General Chemical Engineering", "13. Climate action", "Soil microbial fuel cell; Bioremediation; Modelling; Hexachlorobenzene", "Hexachlorobenzene", "/dk/atira/pure/subjectarea/asjc/1600/1603; name=Electrochemistry", "01 natural sciences", "7. Clean energy", "Bioremediation", "Modelling", "6. Clean water", "0105 earth and related environmental sciences", "12. Responsible consumption"]}, "links": [{"href": "https://iris.unica.it/bitstream/11584/303841/5/pdf24_merged.pdf"}, {"href": "https://doi.org/10.1016/j.electacta.2020.137568"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Electrochimica%20Acta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.electacta.2020.137568", "name": "item", "description": "10.1016/j.electacta.2020.137568", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.electacta.2020.137568"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-01T00:00:00Z"}}, {"id": "10.1016/j.electacta.2021.138636", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:11Z", "type": "Journal Article", "created": "2021-05-22", "title": "Simultaneous removal of hydrocarbons and sulfate from groundwater using a \u201cbioelectric well\u201d", "description": "Abstract   Petroleum hydrocarbons (PHs) are often found in groundwater due to human activities like accidental spills, causing health and environmental risks, and requiring remediation. Microbial Electrochemical Technologies (METs) have emerged as a promising alternative to conventional bioremediation techniques for the treatment of PH-contaminated groundwater. However, the field-application of these promising sustainable as well as cost-effective technologies is still scarce. One major reason is the lack of scalable reactor configurations. Herein, an upgraded version of the \u201cbioelectric well\u201d, a novel tubular bioelectrochemical reactor that can be installed directly within a groundwater well, was tested for the simultaneous removal of oxidableoxidizable (i.e., toluene and other PH) and reducible (i.e., sulfate) compounds from a real contaminated groundwater.  After a proof-of-concept study in batch mode, the system was operated in continuous-flow mode for 48 days with the anode polarized at 0.2\u00a0V vs. SHE and a hydraulic retention time of 11\u00a0h. In these conditions, a steady-state removal rate of toluene as high as 31\u00a0\u00b1\u00a02\u00a0mg L\u22121 d\u22121 was achieved, which was more than double the value observed with the open circuit potential (OCP) control and one of the highest reported in literature. The electrode polarization went along with a higher abundance of key-functional genes involved in toluene degradation. This was not only showing its clear functional connection to the microbial metabolism, but further allowed to identify the involved electrogenic biodegradation pathway. In addition, the system simultaneously removed sulfate (30\u00a0\u00b1\u00a01\u00a0mg L\u22121 d\u22121), with bacteria likely using the H2 generated at the cathode as electron donor. Nevertheless, the apparent sulfate removal rate in the polarized and in the OCP runs was similar. The analysis of the microbial communities evidenced a high abundance of the genus Chlorobium in the effluent of the polarized run. These microorganisms were probably responsible for the continuous oxidative regeneration of sulfate from the sulfide produced at the cathode by sulfate-reducing bacteria. This phenomenon probably hindered the overall removal of sulfate by the bioelectrochemical system.", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Microbial electrochemical technologies", "Groundwater remediation", "bioremediation; groundwater remediation; microbial electrochemical technologies; petroleum hydrocarbons; toluene", "Petroleum hydrocarbons", "Bioremediation", "6. Clean water", "Toluene"]}, "links": [{"href": "https://doi.org/10.1016/j.electacta.2021.138636"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Electrochimica%20Acta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.electacta.2021.138636", "name": "item", "description": "10.1016/j.electacta.2021.138636", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.electacta.2021.138636"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-08-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2021.150433", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:56Z", "type": "Journal Article", "created": "2021-09-20", "title": "Electrochemical water softening as pretreatment for nitrate electro bioremediation", "description": "Open AccessThe dataset contains the raw data of the figures and tables reported in the open access publication 'Ceballos-Escalera, A., Pous, N., Balaguer, M.D., Puig, S., 2022. Electrochemical water softening as pretreatment for nitrate electro bioremediation. Sci. Total Environ. 806, 150433. https://doi.org/10.1016/J.SCITOTENV.2021.150433'.", "keywords": ["Nitrate-contaminated groundwater; Hardness removal; Denitrifying bioelectrochemical system; Polarity reversal", "Nitrates", "02 engineering and technology", "Bioremediaci\u00f3", "01 natural sciences", "6. Clean water", "12. Responsible consumption", "Bioelectrochemistry", "Biodegradation", " Environmental", "Water Softening", "Aig\u00fces subterr\u00e0nies -- Contaminaci\u00f3", "Denitrification", "Groundwater -- Pollution", "Desnitrificaci\u00f3", "0210 nano-technology", "Groundwater", "Bioremediation", "Water Pollutants", " Chemical", "Bioelectroqu\u00edmica", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2021.150433"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2021.150433", "name": "item", "description": "10.1016/j.scitotenv.2021.150433", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2021.150433"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-01T00:00:00Z"}}, {"id": "10.1016/j.biteb.2022.100975", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:15:56Z", "type": "Journal Article", "created": "2022-02-05", "title": "Unveiling microbial electricity driven anoxic ammonium removal", "description": "Microbial electricity-driven anoxic ammonium removal could remove ammonium from wastewater without the presence of oxygen (aeration) using electricity. This study aims at unveiling the potential biologic pathways for the bioelectrochemical oxidation of ammonium to dinitrogen gas in an anaerobic bioelectrochemical system (BES). Known intermediate metabolites of this process (hydroxylamine, nitrite and nitrate) were monitored in two BES replicates. Ammonium was fully oxidized to dinitrogen gas without intermediates accumulation in the anodic chamber. Achromobacter sp. was the most abundant microorganism (up to 60%, according to sequence reads) in the mixed community. Hydroxylamine and nitrite oxidation were electroactive processes, reinforcing the role of the anodic electrode as the electron acceptor for ammonium oxidation. Taking it all together, ammonium can be removed in BES by a combination of different bio/electrochemical processes. A deeper understanding on how the different metabolisms are coupled together is required for increasing the current ammonium removal rates This work was funded through the European Union's Horizon 2020      project ELECTRA [no. 826244]. M. O-A. was supported by a grant from      University of Girona (IFUdG2018/50). S.P is a Serra H\u00fanter Fellow      (UdG-AG-575) and acknowledges the funding from the ICREA Academia      award. LEQUiA [2017-SGR-1552] and Ecoaqua [2017SGR- 548] have      been recognized as consolidated research groups by the Catalan      Governmen Open Access funding provided thanks to the CRUE-CSIC agreement with Elsevier", "keywords": ["Sewage -- Purification -- Nitrogen removal", "0301 basic medicine", "Bioelectrochemistry", "0303 health sciences", "03 medical and health sciences", "Aig\u00fces residuals -- Depuraci\u00f3 -- Desnitrificaci\u00f3", "13. Climate action", "Bioremediaci\u00f3", "Bioremediation", "6. Clean water", "Bioelectroqu\u00edmica"]}, "links": [{"href": "https://doi.org/10.1016/j.biteb.2022.100975"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biteb.2022.100975", "name": "item", "description": "10.1016/j.biteb.2022.100975", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biteb.2022.100975"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-01T00:00:00Z"}}, {"id": "10.1016/j.ese.2020.100013", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:16Z", "type": "Journal Article", "created": "2020-01-13", "title": "Microbial electrochemistry for bioremediation", "description": "Lack of suitable electron donors or acceptors is in many cases the key reason for pollutants to persist in the environment. Externally supplementation of electron donors or acceptors is often difficult to control and/or involves chemical additions with limited lifespan, residue formation or other adverse side effects. Microbial electrochemistry has evolved very fast in the past years - this field relates to the study of electrochemical interactions between microorganisms and solid-state electron donors or acceptors. Current can be supplied in such so-called bioelectrochemical systems (BESs) at low voltage to provide or extract electrons in a very precise manner. A plethora of metabolisms can be linked to electrical current now, from metals reductions to denitrification and dechlorination. In this perspective, we provide an overview of the emerging applications of BES and derived technologies towards the bioremediation field and outline how this approach can be game changing.", "keywords": ["0301 basic medicine", "AUTOTROPHIC DENITRIFICATION", "elecetrobioremediation", "Bioremediaci\u00f3", "FUEL-CELLS", "Environmental technology. Sanitary engineering", "Microbial biotechnology", "01 natural sciences", "POLYCYCLIC AROMATIC-HYDROCARBONS", "03 medical and health sciences", "WASTE-WATER", "DECHLORINATION", "TD Environmental technology. Sanitary engineering", "Electrochemistry", "POLLUTANTS", "GE1-350", "TD1-1066", "0105 earth and related environmental sciences", "NITRATE-CONTAMINATED GROUNDWATER", "ENVIRONMENTAL REMEDIATION", "Q Science (General)", "QR Microbiology", "NITROGEN REMOVAL", "6. Clean water", "Environmental sciences", "Electroqu\u00edmica", "ORGANIC", "BIOELECTROCHEMICAL SYSTEMS", "13. Climate action", "Earth and Environmental Sciences", "Perspective", "Biotecnologia microbiana", "Bioremediation"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/540323/1/1-s2.0-S2666498420300053-main.pdf"}, {"href": "https://doi.org/10.1016/j.ese.2020.100013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Ecotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ese.2020.100013", "name": "item", "description": "10.1016/j.ese.2020.100013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ese.2020.100013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-01T00:00:00Z"}}, {"id": "10.1016/j.ese.2022.100171", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:16Z", "type": "Journal Article", "created": "2022-04-02", "title": "Coupling of bioelectrochemical toluene oxidation and trichloroethene reductive dechlorination for single-stage treatment of groundwater containing multiple contaminants", "description": "Bioremediation of groundwater contaminated by a mixture of aromatic hydrocarbons and chlorinated solvents is typically challenged because these contaminants are degraded via distinctive oxidative and reductive pathways, thus requiring different amendments and redox conditions. Here, we provided the proof-of-concept of a single-stage treatment of synthetic groundwater containing toluene and trichloroethene (TCE) in a tubular bioelectrochemical reactor, known as a 'bioelectric well'. Toluene was degraded by a microbial bioanode (up to 150\u00a0\u03bcmol\u00a0L-1\u00a0d-1) with a polarized graphite anode (+0.2\u00a0V vs. SHE) serving as the terminal electron acceptor. The electric current deriving from microbially-driven toluene oxidation resulted in (abiotic) hydrogen production (at a stainless-steel cathode), which sustained the reductive dechlorination of TCE to less-chlorinated intermediates (i.e., cis-DCE, VC, and ETH), at a maximum rate of 500 \u03bceq L-1 d-1, in the bulk of the reactor. A phylogenetic and functional gene-based analysis of the 'bioelectric well' confirmed the establishment of a microbiome harboring the metabolic potential for anaerobic toluene oxidation and TCE reductive dechlorination. However, Toluene degradation and current generation were found to be rate-limited by external mass transport phenomena, thus indicating the existing potential for further process optimization.", "keywords": ["0301 basic medicine", "0303 health sciences", "bioelectric well; dehalococcoides mccartyi; electrobioremediation; groundwater remediation; toluene; trichloroethene", "Bioelectric well", "Environmental technology. Sanitary engineering", "6. Clean water", "Trichloroethene", "Environmental sciences", "03 medical and health sciences", "Groundwater remediation", "Electrobioremediation", "GE1-350", "Dehalococcoides mccartyi", "TD1-1066", "Toluene", "Original Research"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/475036/1/Coupling%20of%20bioelectrochemical%20toluene%20oxidation%20and%20trichloroethene%20reductive%20dechlorination%20for%20single-stage%20treatment%20of%20groundwater%20containing%20multiple%20contaminants.pdf"}, {"href": "https://iris.uniroma1.it/bitstream/11573/1661829/2/Cruz-Viggi_Coupling-bioelectrochemical_2022.pdf"}, {"href": "https://doi.org/10.1016/j.ese.2022.100171"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Ecotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ese.2022.100171", "name": "item", "description": "10.1016/j.ese.2022.100171", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ese.2022.100171"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-07-01T00:00:00Z"}}, {"id": "10.1016/j.jece.2020.104657", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:40Z", "type": "Journal Article", "created": "2020-10-24", "title": "Reductive/oxidative sequential bioelectrochemical process for Perchloroethylene (PCE) removal: effect of the applied reductive potential and microbial community characterization", "description": "Abstract   In this paper, a bioelectrochemical process has been developed by the combination of two membrane-less reactors equipped with an internal graphite granules counterelectrode for the perchloroethylene (PCE) removal through a reductive/oxidative sequence. In the reductive reactor, the cathodic chamber supplied the reducing power to PCE dechlorinating biomass while a rutile electrode promoted the aerobic dechlorination of the less chlorinated PCE byproducts by oxygen in situ evolution. Two potentiostatic conditions, -350 and -550 mV vs SHE, were tested on the reductive reactor, which showed the capability to completely reduce the PCE into vinyl chloride (VC) and ethylene (Eth). These compounds were completely removed by the oxidative reactor with an average VC and Eth removal efficiency of 94 \u00b1 1% and 98 \u00b1 1%. The -350 mV vs SHE condition resulted in the higher coulombic efficiency for the reductive dechlorination which reached 22 \u00b1 7 % while by increasing the reductive potential to -550 mV the coulombic efficiency drop down to 6 \u00b1 1 % in favor of the methanogenesis reaction. Dehalococcoides mccartyi was found at high abundance in the reducing reactor while a heterogeneous bacterial consortium was observed in the oxidative reactor. Microbiome characterization of the reductive and oxidative reactors showed the concomitant presence of different redox niches in each compartment suggesting that the exchange of ionic species between the electrode and the counterelectrode allowed the co-existence of both reducing and oxidative reactions.", "keywords": ["0301 basic medicine", "0303 health sciences", "EC", "reductive dechlorination; oxidative dechlorination; bioremediation; bioelectrochemical systems; chlorinated aliphatic hydrocarbons; groundwater remediation", "Reductive dechlorination", "Process Chemistry and Technology", "H2020", "Pollution", "Horizon 2020 Framework Programme", "6. Clean water", "Research and Innovation action", "Bioelectrochemical systems", "03 medical and health sciences", "bioremediation", "Chemical Engineering (miscellaneous)", "European Commission", "Waste Management and Disposal"]}, "links": [{"href": "https://doi.org/10.1016/j.jece.2020.104657"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Chemical%20Engineering", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jece.2020.104657", "name": "item", "description": "10.1016/j.jece.2020.104657", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jece.2020.104657"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-01T00:00:00Z"}}, {"id": "10.1016/j.jece.2022.107799", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:41Z", "type": "Journal Article", "created": "2022-04-27", "title": "\"Syntrophy drives the microbial electrochemical oxidation of toluene in a continuous-flow \"\"bioelectric well\"\"\"", "description": "Microbial electrochemical technologies (MET) are promising for the remediation of groundwater pollutants such as petroleum hydrocarbons (PH). Indeed, MET can provide virtually inexhaustible electron donors or acceptors directly in the subsurface environment. However, the degradation mechanisms linking contaminants removal to electric current flow are still largely unknown, hindering the development of robust design criteria. Here, we analysed the degradation of toluene, a model PH, in a bioelectrochemical reactor known as 'bioelectric well'operated in continuous-flow mode at various influent toluene concentrations. With increasing concentration of toluene, the removal rate increased while the current tended to a plateau, hence the columbic efficiency decreased. Operation at open circuit confirmed that the bioelectrochemical degradation of toluene proceeded via a syntrophic pathway involving cooperation between different microbial populations. First of all, hydrocarbon degraders quickly converted toluene into metabolic intermediates probably by breaking the aromatic ring upon fumarate addition. Subsequently, fermentative bacteria converted these intermediates into volatile fatty acids (VFA) and likely also H2, which were then used as substrates by electroactive microorganisms forming the anodic biofilm. As toluene degradation is faster than subsequent conversion steps, the increase in intermediate concentration could not result in a current increase. This work provides valuable insights on the syntrophic degradation of BTEX, which are essential for the application of microbial electrochemical system to groundwater remediation of petroleum hydrocarbons.", "keywords": ["Microbial electrochemical technologies", "13. Climate action", "Groundwater remediation", "bioremediation; groundwater remediation; microbial electrochemical technologies; petroleum hydrocarbons; toluene", "Petroleum hydrocarbons", "01 natural sciences", "Bioremediation", "6. Clean water", "Toluene", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/475025/1/Syntrophy%20drives%20the%20microbial%20electrochemical%20oxidation%20of%20toluene%20in%20a%20continuous-flow%20%e2%80%9cbioelectric%20well%e2%80%9d.pdf"}, {"href": "https://iris.uniroma1.it/bitstream/11573/1661830/2/Tucci_Syntrophy-drives-microbial_2022.pdf"}, {"href": "https://doi.org/10.1016/j.jece.2022.107799"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Chemical%20Engineering", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jece.2022.107799", "name": "item", "description": "10.1016/j.jece.2022.107799", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jece.2022.107799"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-06-01T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2020.123676", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:43Z", "type": "Journal Article", "created": "2020-08-14", "title": "Simultaneous adsorption and biodegradation of trichloroethylene occurs in a biochar packed column treating contaminated landfill leachate", "description": "Trichloroethylene (TCE) is a human carcinogen that is commonly found in landfill leachate as a result of anthropogenic activities. Contaminated leachate plumes may be intercepted prior to reaching groundwater and treated in situ using permeable reactive barriers (PRB). This study used a packed column system containing herbal pomace and spruce biochar, previously shown to have TCE adsorptive capabilities, to investigate the feasibility of using pyrolysed waste as a fill material in a PRB. Influent containing raw or autoclaved landfill leachate was used to investigate the potential for environmental micro-organisms to establish a TCE-dechlorinating biofilm on the biochar, in order to prolong the operational life span of the system. TCE removal \u2265 99.7 was observed by both spruce and herbal pomace based biochars. No dichloroethylene (DCE) isomers were present in the column effluents, but cis-1,2 DCE was adsorbed to the biochar treating raw landfill leachate, indicating that dechlorination was occurring biologically in these columns. Known microbial species that are individually capable of complete dechlorination of TCE to ethene were not detected by 16S rRNA gene sequencing, but several species capable of partial TCE dechlorination (Desulfitobacterium spp., Sulfurospirillium spp. and Desulfuromonas spp) were present in the biofilms of the columns treating raw landfill leachate. These data demonstrate that biochar from waste material may be capable of supporting a dechlorinating biofilm to promote bioremediation of TCE.", "keywords": ["Permeable Reactive Barrier", "Waste reuse", "01 natural sciences", "6. Clean water", "Trichloroethylene", "12. Responsible consumption", "3. Good health", "Biochar", "Biodegradation", " Environmental", "13. Climate action", "Charcoal", "RNA", " Ribosomal", " 16S", "Humans", "Adsorption", "Water Pollutants", " Chemical", "Bioremediation", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.jhazmat.2020.123676"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2020.123676", "name": "item", "description": "10.1016/j.jhazmat.2020.123676", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2020.123676"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2022.157325", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:56Z", "type": "Journal Article", "created": "2022-07-14", "title": "Passive electrobioremediation approaches for enhancing hydrocarbons biodegradation in contaminated soils,", "description": "Electrobioremediation technologies hold considerable potential for the treatment of soils contaminated by petroleum hydrocarbons (PH), since they allow stimulating biodegradation processes with no need for subsurface chemicals injection and with little to no energy consumption. Here, a microbial electrochemical snorkel (MES) was applied for the treatment of a soil contaminated by hydrocarbons. The MES consists of direct coupling of a microbial anode with a cathode, being a single conductive, non-polarized material positioned suitably to create an electrochemical connection between the anoxic zone (the contaminated soil) and the oxic zone (the overlying oxygenated water). Soil was also supplemented with electrically conductive particles of biochar as a strategy to construct a conductive network with microbes in the soil matrix, thus extending the radius of influence of the snorkel. The results of a comprehensive suite of chemical, microbiological and ecotoxicological analyses evidenced that biochar addition, rather than the presence of a snorkel, was the determining factor in accelerating PH removal from contaminated soils, possibly accelerating syntrophic and/or cooperative metabolisms involved in the degradation of PH. The enhancement of biodegradation was mirrored by an increased abundance of anaerobic and aerobic microorganisms known to be involved in the degradation of PH and related functional genes. Plant ecotoxicity assays confirmed a reduction of soils toxicity in treatments receiving electrically conductive biochar.", "keywords": ["02 engineering and technology", "01 natural sciences", "Hydrocarbons", "6. Clean water", "Biochar", "Soil", "Biodegradation", " Environmental", "Petroleum", "Soil Pollutants", "Contaminated soil", "Microbial electrochemical snorkel", "biochar; bioelectrochemical system; bioremediation; contaminated soil; microbial electrochemical snorkel; petroleum hydrocarbons", "0210 nano-technology", "Petroleum hydrocarbons", "Bioremediation", "Soil Microbiology", "Bioelectrochemical system", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2022.157325"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2022.157325", "name": "item", "description": "10.1016/j.scitotenv.2022.157325", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2022.157325"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-11-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2023.161600", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:57Z", "type": "Journal Article", "created": "2023-01-18", "title": "Risk reductions during pyrene biotransformation and mobilization in a model plant-bacteria-biochar system", "description": "The productive application of motile microorganisms for degrading hydrophobic contaminants in soil is one of the most promising processes in modern remediation due to its sustainability and low cost. However, the incomplete biodegradation of the contaminants and the formation of the intermediary metabolites in the process may increase the toxicity in soil during bioremediation, and motile inoculants may mobilize the pollutants through biosorption. Therefore, controlling these factors should be a fundamental part of soil remediation approaches. The aim of this study was to evaluate the sources of risk associated with the cometabolism-based transformation of 14C-labeled pyrene by inoculated Pseudomonas putida G7 and identify ways to minimize risk. Our model scenario examined the increase in bioaccessibility to a distant source of contamination facilitated by sunflower (Helianthus annuus L.) roots. A biochar trap for mobilized pollutant metabolites and bacteria has also been employed. The experimental design consisted of pots filled with a layer of sand with 14C-labeled pyrene (88 mg kg-1) as a contamination focus located several centimeters from the inoculation point. Half of the pots included a biochar layer at the bottom. The pots were incubated in a greenhouse with sunflower plants and P. putida G7 bacteria. Pots with sunflower plants showed a higher biodegradation of pyrene, its mobilization as metabolites through the percolate and the roots, and bacterial mobilization toward the source of contamination, also resulting in increased pyrene transformation. In addition, the biochar layer efficiently reduced the concentrations of pyrene metabolites collected in the leachates. Therefore, the combination of plants, motile bacteria and biochar safely reduced the risk caused by the biological transformation of pyrene.", "keywords": ["Risk", "2. Zero hunger", "570", "Pyrenes", "Bacteria", "04 agricultural and veterinary sciences", "Plants", "01 natural sciences", "Polycyclic aromatic hydrocarbons", "6. Clean water", "Sunflower", "Soil", "Biodegradation", " Environmental", "13. Climate action", "Biodegradation", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Polycyclic Aromatic Hydrocarbons", "Bioremediation", "Biotransformation", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2023.161600"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2023.161600", "name": "item", "description": "10.1016/j.scitotenv.2023.161600", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2023.161600"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-04-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2024.176720", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:58Z", "type": "Journal Article", "created": "2024-10-07", "title": "Environmental and socio-economic evaluation of a groundwater bioremediation technology using social Cost-Benefit Analysis: Application to an in-situ metal(loid) precipitation case study", "description": "Bioremediation can be an alternative or complementary approach to conventional soil and water treatment technologies. Determining the environmental and socio-economic impacts of bioremediation is important but rarely addressed. This work presents a comprehensive sustainability assessment for a specific groundwater bioremediation case study based on In-situ Metal(loid) Precipitation (ISMP) by conducting a social Cost-Benefit Analysis (CBA) using two different approaches: environmental Life Cycle Costing (eLCC) and Impact Pathway Approach (IPA). Externalities are calculated in two ways: i) using Environmental Prices (EP) to monetize Life Cycle Assessment (LCA) results and metal(loid)s removed at field scale, and ii) following the IPA steps to determine the social costs avoided by removing arsenic contamination at full scale. The results show that, in the baseline scenario, the project is not socio-economically viable in both cases as the Net Present Value (NPV) is -129,512.61 \u20ac and\u00a0-\u00a0415,185,140 \u20ac respectively. Sensitivity and scenario analyses are performed to identify the key parameters and actions needed to reach a positive NPV. For instance, increasing the amount of water treated per year to 90 m3 and assuming a 20\u00a0% increase in operation costs and a 60\u00a0% increase in construction costs can make the project socio-economically viable at the field scale, while a reduction in the social discount rate from a 4\u00a0% to a 2\u00a0% can lead to a positive NPV at the full scale. The approaches proposed in this work may be useful for practitioners and policymakers when evaluating the environmental and socio-economic impacts of bioremediation technologies at different scales and regions, as well as human health impacts caused by contaminants at the current legal limits.", "keywords": ["Bioqu\u00edmica", "570", "550", "Environmental Life Cycle Costing", "Cost-Benefit Analysis", "Life Cycle Assessment", "Biochemistry", "Biorremediaci\u00f3n", "Water Purification", "Biodegradation", " Environmental", "Metals", "mpact Pathway Approach", "Social Cost-Benefit Analysis", "Groundwater", "Bioremediation", "Water Pollutants", " Chemical"], "contacts": [{"organization": "Ib\u00e1\u00f1ez, Jes\u00fas, P\u00e9rez-de-Mora, Alfredo, Santiago-Herrera, Mario, Belloncle, Benjamine, de Wilde, Herwig, Martel-Mart\u00edn, Sonia, Blanco-Alc\u00e1ntara, David, Barros, Roc\u00edo,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2024.176720"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2024.176720", "name": "item", "description": "10.1016/j.scitotenv.2024.176720", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2024.176720"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "10.3389/fenvs.2021.624070", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:46Z", "type": "Journal Article", "created": "2021-03-15", "title": "Soil Microbiome Structure and Function in Ecopiles Used to Remediate Petroleum-Contaminated Soil", "description": "<p>The soil microbiome consists of a vast variety of microorganisms which contribute to essential ecosystem services including nutrient recycling, protecting soil structure, and pathogen suppression. Recalcitrant organic compounds present in soils contaminated with fuel oil can lead to a decrease in functional redundancy within soil microbiomes. Ecopiling is a passive bioremediation technique involving biostimulation of indigenous hydrocarbon degraders, bioaugmentation through inoculation with known petroleum-degrading consortia, and phytoremediation. The current study investigates the assemblage of soil microbial communities and pollutant-degrading potential in soil undergoing the Ecopiling process, through the amplicon marker gene and metagenomics analysis of the contaminated soil. The analysis of key community members including bacteria, fungi, and nematodes revealed a surprisingly diverse microbial community composition within the contaminated soil. The soil bacterial community was found to be dominated by Alphaproteobacteria (60\uffe2\uff80\uff9370%) with the most abundant genera such as Lysobacter, Dietzia, Pseudomonas, and Extensimonas. The fungal community consisted mainly of Ascomycota (50\uffe2\uff80\uff9370% relative abundance). Soil sequencing data allowed the identification of key enzymes involved in the biodegradation of hydrocarbons, providing a novel window into the function of individual bacterial groups in the Ecopile. Although the genus Lysobacter was identified as the most abundant bacterial genus (11\uffe2\uff80\uff9346%) in all of the contaminated soil samples, the metagenomic data were unable to confirm a role for this group in petrochemical degradation. Conversely, genera with relatively low abundance such as Dietzia (0.4\uffe2\uff80\uff939.0%), Pusillimonas (0.7\uffe2\uff80\uff932.3%), and Bradyrhizobium (0.8\uffe2\uff80\uff931.8%) did possess genes involved in aliphatic or aromatic compound degradation.</p", "keywords": ["0301 basic medicine", "2. Zero hunger", "metagenomics", "microbiome", "phytoremediation", "15. Life on land", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "6. Clean water", "Phytoremediation", "12. Responsible consumption", "Environmental sciences", "Ecopiling", " bioremediation", " phytoremediation", " microbiome", " metagenomics", "Ecopiling", "03 medical and health sciences", "bioremediation", "13. Climate action", "GE1-350", "Microbiome", "Metagenomics", "Bioremediation"]}, "links": [{"href": "https://doi.org/10.3389/fenvs.2021.624070"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Environmental%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fenvs.2021.624070", "name": "item", "description": "10.3389/fenvs.2021.624070", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fenvs.2021.624070"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-03-15T00:00:00Z"}}, {"id": "10.1016/j.watres.2020.116748", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:17:24Z", "type": "Journal Article", "created": "2020-12-16", "title": "Electro-bioremediation of nitrate and arsenite polluted groundwater.", "description": "The coexistence of different pollutants in groundwater is a common threat. Sustainable and resilient technologies are required for their treatment. The present study aims to evaluate microbial electrochemical technologies (METs) for treating groundwater contaminated with nitrate (NO3-) while containing arsenic (in form of arsenite (As(III)) as a co-contaminant. The treatment was based on the combination of nitrate reduction to dinitrogen gas and arsenite oxidation to arsenate (exhibiting less toxicity, solubility, and mobility), which can be removed more easily in further post-treatment. We operated a bioelectrochemical reactor at continuous-flow mode with synthetic contaminated groundwater (33 mg N-NO3- L-1 and 5 mg As(III) L-1) identifying the key operational conditions. Different hydraulic retention times (HRT) were evaluated, reaching a maximum nitrate reduction rate of 519 g N-NO3- m3Net Cathodic Compartment d-1 at HRT of 2.3 h with a cathodic coulombic efficiency of around 100 %. Simultaneously, arsenic oxidation was complete at all HRT tested down to 1.6 h reaching an oxidation rate of up to 90 g As(III) m-3Net Reactor Volume d -1. Electrochemical and microbiological characterization of single granules suggested that arsenite at 5 mg L-1 did not have an inhibitory effect on a denitrifying biocathode mainly represented by Sideroxydans sp. Although the coexistence of abiotic and biotic arsenic oxidation pathways was shown to be likely, microbial arsenite oxidation linked to denitrification by Achromobacter sp. was the most probable pathway. This research paves the ground towards a real application for treating groundwater with widespread pollutants.", "keywords": ["Nitrates", "Arsenites", "0211 other engineering and technologies", "02 engineering and technology", "Bioremediaci\u00f3", "01 natural sciences", "6. Clean water", "Arsenic", "12. Responsible consumption", "Bioelectrochemistry", "Biodegradation", " Environmental", "13. Climate action", "Aig\u00fces subterr\u00e0nies -- Contaminaci\u00f3", "Denitrification", "Groundwater -- Pollution", "Desnitrificaci\u00f3", "Groundwater", "Oxidation-Reduction", "Bioremediation", "Water Pollutants", " Chemical", "Bioelectroqu\u00edmica", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.watres.2020.116748"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2020.116748", "name": "item", "description": "10.1016/j.watres.2020.116748", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2020.116748"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-01T00:00:00Z"}}, {"id": "10.1021/acsomega.1c03001", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:17:32Z", "type": "Journal Article", "created": "2021-09-22", "title": "Control of sulfate and nitrate reduction by setting hydraulic retention time and applied potential on a membraneless microbial electrolysis cell for perchloroethylene removal", "description": "A membraneless microbial electrolysis cell (MEC) has been developed for perchloroethylene (PCE) removal through the reductive dechlorination reaction. The MEC consists of a tubular reactor of 8.24 L equipped with a graphite-granule working electrode which stimulates dechlorinating microorganisms while a graphite-granule cylindrical envelopment contained in a plastic mesh constituted the counter electrode of the MEC. Synthetic PCE-contaminated groundwater has been used as the feeding solution to test the nitrate and sulfate reduction reactions on the MEC performance at different hydraulic retention times (HRTs) (4.1, 1.8, and 1.2) and different cathodic potentials [-350, -450, and -650 mV vs standard hydrogen electrode (SHE)]. The HRT decrease from 4.1 to 1.8 d promoted a considerable increase in sulfate removal from 38 \u00b1 11 to 113 \u00b1 26 mg/Ld with a consequent current increase, while a shorter HRT of 1.2 d caused a partial inhibition of sulfate reduction with a consequent current decrease from -99 \u00b1 3 to -52 \u00b1 6 mA. Similarly, the cathodic potential investigation showed a direct correlation of current generation and sulfate removal in which the utilization of a cathodic potential of -350 mV versus SHE allowed for an 80% decrease in the sulfate removal rate with a consequent current decrease from -163 \u00b1 7 to 41 \u00b1 5 mA. The study showed the possibility to mitigate the energy consumption of the process by avoiding side reactions and current generation, through the selection of an appropriate HRT and applied cathodic potential.", "keywords": ["Chemistry", "reductive dechlorination; perchloroethylene; bioelectrochemical systems; bioremediation", "QD1-999", "01 natural sciences", "7. Clean energy", "6. Clean water", "0105 earth and related environmental sciences", "12. Responsible consumption"]}, "links": [{"href": "https://iris.uniroma1.it/bitstream/11573/1570432/1/Dell%e2%80%99Armi_Control-sulfate-nitrate_2021.pdf"}, {"href": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.1c03001"}, {"href": "https://doi.org/10.1021/acsomega.1c03001"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ACS%20Omega", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acsomega.1c03001", "name": "item", "description": "10.1021/acsomega.1c03001", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acsomega.1c03001"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-09-22T00:00:00Z"}}, {"id": "10.1186/s13568-024-01764-7", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:22Z", "type": "Journal Article", "created": "2024-09-28", "title": "Metagenomic analyses of a consortium for the bioremediation of hydrocarbons polluted soils", "description": "Abstract<p>A bacterial consortium was isolated from a soil in Noblejas (Toledo, Spain) with a long history of mixed hydrocarbons pollution, by enrichment cultivation. Serial cultures of hydrocarbons polluted soil samples were grown in a minimal medium using diesel (1\uffc2\uffa0mL/L) as the sole carbon and energy source. The bacterial composition of the Noblejas Consortium (NC) was determined by sequencing 16S rRNA gene amplicon libraries. The consortium contained around 50 amplicon sequence variants (ASVs) and the major populations belonged to the genera Pseudomonas, Enterobacter, Delftia, Stenotrophomonas, Achromobacter, Acinetobacter, Novosphingobium, Allorhizobium-Neorhizobium-Rhizobium, Ochrobactrum and Luteibacter. All other genera were below 1%. Metagenomic analysis of NC has shown a high abundance of genes encoding enzymes implicated in aliphatic and (poly) aromatic hydrocarbons degradation, and almost all pathways for hydrocarbon degradation are represented. Metagenomic analysis has also allowed the construction of metagenome assembled genomes (MAGs) for the major players of NC. Metatranscriptomic analysis has shown that several of the ASVs are implicated in hydrocarbon degradation, being Pseudomonas, Acinetobacter and Delftia the most active populations.</p", "keywords": ["metagenomics", "Bacterial consortium; Bioremediation; Metagenomics; Metatranscriptomics; Total petroleum hydrocarbons", "metatranscriptomics", "Bacterial consortium", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Microbiology", "QR1-502", "Total petroleum hydrocarbons", "total petroleum hydrocarbons", "bioremediation", "Original Article", "Metagenomics", "Bioremediation", "TP248.13-248.65", "Metatranscriptomics", "Biotechnology"]}, "links": [{"href": "https://doi.org/10.1186/s13568-024-01764-7"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/AMB%20Express", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s13568-024-01764-7", "name": "item", "description": "10.1186/s13568-024-01764-7", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s13568-024-01764-7"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-09-28T00:00:00Z"}}, {"id": "10.3389/fpls.2024.1325048", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-25T16:20:51Z", "type": "Journal Article", "created": "2024-02-02", "title": "Flavonoids influence key rhizocompetence traits for early root colonization and PCB degradation potential of Paraburkholderia xenovorans LB400", "description": "Introduction<p>Flavonoids are among the main plant root exudation components, and, in addition to their role in symbiosis, they can broadly affect the functionality of plant-associated microbes: in polluted environments, for instance, flavonoids can induce the expression of the enzymatic degradative machinery to clean-up soils from xenobiotics like polychlorinated biphenyls (PCBs). However, their involvement in root community recruitment and assembly involving non-symbiotic beneficial interactions remains understudied and may be crucial to sustain the holobiont fitness under PCB stress.</p>Methods<p>By using a set of model pure flavonoid molecules and a natural blend of root exudates (REs) with altered flavonoid composition produced by Arabidopsis mutant lines affected in flavonoid biosynthesis and abundance (null mutant tt4, flavonoid aglycones hyperproducer tt8, and flavonoid conjugates hyperaccumulator ttg), we investigated flavonoid contribution in stimulating rhizocompetence traits and the catabolic potential of the model bacterial strain for PCB degradation Paraburkholderia xenovorans LB400.</p>Results<p>Flavonoids influenced the traits involved in bacterial recruitment in the rhizoplane by improving chemotaxis and motility responses, by increasing biofilm formation and by promoting the growth and activation of the PCB-degradative pathway of strain LB400, being thus potentially exploited as carbon sources, stimulating factors and chemoattractant molecules. Indeed, early rhizoplane colonization was favored in plantlets of the tt8 Arabidopsis mutant and reduced in the ttg line. Bacterial growth was promoted by the REs of mutant lines tt4 and tt8 under control conditions and reduced upon PCB-18 stress, showing no significant differences compared with the WT and ttg, indicating that unidentified plant metabolites could be involved. PCB stress presumably altered the Arabidopsis root exudation profile, although a sudden \uffe2\uff80\uff9ccry-for-help\uffe2\uff80\uff9d response to recruit strain LB400 was excluded and flavonoids appeared not to be the main determinants. In the in vitro plant\uffe2\uff80\uff93microbe interaction assays, plant growth promotion and PCB resistance promoted by strain LB400 seemed to act through flavonoid-independent mechanisms without altering bacterial colonization efficiency and root adhesion pattern.</p>Discussions<p>This study further contributes to elucidate the vast array of functions provided by flavonoids in orchestrating the early events of PCB-degrading strain LB400 recruitment in the rhizosphere and to support the holobiont fitness by stimulating the catabolic machinery involved in xenobiotics decomposition and removal.</p", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "polychlorinated biphenyls", "plant-microbe interactions", "Plant culture", "Plant Science", "cry-for-help", "15. Life on land", "Rhizosphere; Beneficial bacteria; Bioremediation; Plant-microbe interactions; Polychlorinated biphenyls; Cry-for-help", "SB1-1110", "03 medical and health sciences", "bioremediation", "rhizosphere", "beneficial bacteria"], "contacts": [{"organization": "E. Ghitti, E. Rolli, L. Vergani, S. Borin,", "roles": ["creator"]}]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/1027450/2/Ghitti%20et%20al.%2c%202024%20flavonoids%20Paraburkholderia.pdf"}, {"href": "https://doi.org/10.3389/fpls.2024.1325048"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2024.1325048", "name": "item", "description": "10.3389/fpls.2024.1325048", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2024.1325048"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-02-02T00:00:00Z"}}, {"id": "10.21203/rs.3.rs-3537993/v2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:05Z", "type": "Journal Article", "created": "2024-12-01", "title": "Metal(loid) tolerance, accumulation, and phytoremediation potential of wetland macrophytes for multi-metal(loid)s polluted water.", "description": "<title>Abstract</title>         <p>Natural based solutions, notably constructed/artificial wetland treatment systems, rely heavily on identification and use of macrophytes with the ability to tolerate multiple contaminants and grow for an extended period to reduce contamination. The potential to tolerate and remediate metal(loid) contaminated groundwater from an industrial site located in Flanders (Belgium) was assessed for 10 wetland macrophytes (including <italic>Carex riparia, Cyperus longus, Cyperus rotundus, Iris pseudacorus, Juncus effusus, Lythrum salicaria, Menta aquatica, Phragmites australis, Scirpus holoschoenus,</italic> and <italic>Typha angustifolia</italic>). The experiment was conducted under static conditions, where plants were exposed to polluted acidic (pH~4)water, having high level of metal(loid)s for 15 days. Plant biomass, morphology, and metal uptake by roots and shoots were analysed every 5 days for all species. <italic>T. angustifolia</italic> and <italic>S. holoschoenus </italic>produced ~3 and ~1.1 times more dried biomass than the controls, respectively. For <italic>S. holoschoenus, P. australis,</italic> and <italic>T. angustifolia</italic>, no apparent morphological stress symptoms were observed, and plant heights were similar between control and plants exposed to polluted groundwater. Higher concentrations of all metal(loid)s were detected in the roots indicating a potential for phytostabilization of metal(loid)s below the water column. For <italic>J. effusus</italic> and <italic>T. angustifolia</italic>, Cd, Ni, and Zn accumulation was observed higher in the shoots. <italic>S. holoschoenus</italic>, <italic>P. australis,</italic> and <italic>T. angustifolia</italic> are proposed for restoration and phytostabilization strategies in natural and/or constructed wetland and aquatic ecosystems affected by metal(loid) inputs.</p>", "keywords": ["580", "570", "Constructed wetlands", "15. Life on land", "Biorremediaci\u00f3n", "6. Clean water", "Macrophytes", "Agua-Contaminaci\u00f3n", "Biodegradation", " Environmental", "Heavy metals", "Water-Pollution", "Belgium", "Metals", "13. Climate action", "Wetlands", "Metals", " Heavy", "Phytostabilization", "Groundwater", "Bioremediation", "Water Pollutants", " Chemical", "Research Article"]}, "links": [{"href": "https://doi.org/10.21203/rs.3.rs-3537993/v2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.21203/rs.3.rs-3537993/v2", "name": "item", "description": "10.21203/rs.3.rs-3537993/v2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.21203/rs.3.rs-3537993/v2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-12T00:00:00Z"}}, {"id": "10.3303/cet2186063", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:44Z", "type": "Journal Article", "title": "Sequential reductive/oxidative bioelectrochemical process for groundwater perchloroethylene removal", "description": "Chlorinated aliphatic hydrocarbons (CAHs) are common groundwater contaminants, microbial communities naturally present in groundwater can reduce CAHs as perchloroethylene (PCE) and trichloroethylene (TCE) to ethylene through reductive dechlorination (RD) reaction while low chlorinated CAHs like cis-dichloroethylene (cis DCE) and vinyl chloride (VC) can be oxidized by aerobic pathways. A combination of reductive and oxidative dechlorination results an effective strategy for the complete mineralization of CAHs. Bioelectrochemical systems (BES) are innovative processes which can be adopted to stimulate both reductive and oxidative dechlorination biomass through polarized electrodes. The present study describes the performances of a an oxidative bioelectrochemical reactor composed by a membrane-less microbial electrolysis cell (MEC) equipped with an internal graphite counterelectrode. In the oxidative reactor the oxygen provided by a mixed metal oxides (MMO) anode stimulated the oxidative dechlorination of the cisDCE contained in synthetic groundwater. Throughout the experimental period, both reductive and oxidative dechlorination pathways were identified due to presence of an internal counter electrode that acted as electron donor. Reductive and oxidative bioelectrochemical reactions, including anions reduction were determined and their relative contribution to the overall flowing current has been quantified in terms of oxidative and reductive coulombic efficiencies.", "keywords": ["TK7885-7895", "Computer engineering. Computer hardware", "Chemical engineering", "oxidative dechlorination; bioelectrochemical systems; bioremediation", "TP155-156"]}, "links": [{"href": "https://iris.uniroma1.it/bitstream/11573/1560204/1/Zeppilli_Sequential-reductive/oxidative_2021.pdf"}, {"href": "https://doi.org/10.3303/cet2186063"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemical%20Engineering%20Transactions", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3303/cet2186063", "name": "item", "description": "10.3303/cet2186063", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3303/cet2186063"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "10.3389/fenvs.2023.1141562", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:47Z", "type": "Journal Article", "created": "2023-03-09", "title": "Shift of combined ecotoxicity index in petroleum polluted soils during a bacterial remediation", "description": "<p>Introduction: Bioremediation has been shown to be an effective strategy for removing toxic pollutants from the environment, particularly organic chemicals such as petroleum hydrocarbons. This paper investigates the changes in toxicity of petroleum-contaminated soil as a result of microbial remediation processes.</p><p>Methods: Changes in the ecotoxicity of the contaminated soil were examined using a plant, earthworm, enzyme activity and luminescent bacteria toxicity tests.</p><p>Results: The results showed that bioremediation could effectively degrade petroleum hydrocarbon (C10\uffe2\uff80\uff93C40) pollutants. After 42\uffc2\uffa0days of remediation, the petroleum hydrocarbon (C10\uffe2\uff80\uff93C40) content of Group A (bioaugmented polluted wetland soil) decreased from 1.66\uffc2\uffa0g/kg to 1.00\uffc2\uffa0g/kg, and the degradation rate was 40.6%. The petroleum hydrocarbon (C10\uffe2\uff80\uff93C40) content of Group B (bioaugmented polluted farmland soil decreased from 4.00\uffc2\uffa0g/kg to 1.94\uffc2\uffa0g/kg, and the degradation rate was 51.6%. During the microbial remediation progress, the ecological toxicity of petroleum-contaminated soil first increased and then decreased. The photosynthetic pigment content index in the higher plant toxicity test, the earthworm survival index and the soil catalase activity all showed good agreement with the relative luminescence index of extracted DCM/DMSO in the luminescent bacterial toxicity test. The soil toxicity decreased significantly after remediation. Specifically, the photosynthetic pigment content of wheat were inhibited in the soil during the whole process (remediation for 42\uffc2\uffa0days), and decreased to the minimum on remediation day 21. The 7-day and 14-day survival rate of earthworms in Group A and Group B gradually decreased in the soil remediation process, and then gradually increased, survival rate at the end of remediation was higher than at the beginning. Soil catalase activity was significantly negatively correlated with petroleum hydrocarbon (C10\uffe2\uff80\uff93C40) content (\uffe2\uff88\uff920.988, \uffe2\uff88\uff920.989). The ecological toxicity of contaminated soil reached to the maximum on the 21st day of remediation, relative luminosity of luminescent bacteria in dichloromethane/dimethyl sulfoxide extracts from Group A and Group B were 26.3% and 16.3%, respectively.</p><p>Conclusion: Bioremediation could effectively degrade petroleum hydrocarbon (C10\uffe2\uff80\uff93C40) pollutants. Wheat photosynthetic pigment content, earthworm survival rate, soil catalase activity and relative luminescence of luminescent bacteria can better indicate the ecological toxicity of petroleum-contaminated soil in bioremediation process.</p", "keywords": ["petroleum", "Environmental sciences", "2. Zero hunger", "soil enzyme activity", "bioremediation", "ecotoxicity", "13. Climate action", "luminescent bacteria", "GE1-350", "6. Clean water", "soil"]}, "links": [{"href": "https://doi.org/10.3389/fenvs.2023.1141562"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Environmental%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fenvs.2023.1141562", "name": "item", "description": "10.3389/fenvs.2023.1141562", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fenvs.2023.1141562"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-03-09T00:00:00Z"}}, {"id": "10.3389/fmicb.2023.1158130", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-25T16:20:49Z", "type": "Journal Article", "created": "2023-04-21", "title": "Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the process", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Ecopiling is a method for biodegradation of hydrocarbons in soils. It derives from Biopiles, but phytoremediation is added to biostimulation with nitrogen fertilization and bioaugmentation with local bacteria. We have constructed seven Ecopiles with soil heavily polluted with hydrocarbons in Carlow (Ireland). The aim of the study was to analyze changes in the microbial community during ecopiling. In the course of 18\u2009months of remediation, total petroleum hydrocarbons values decreased in 99 and 88% on average for aliphatics and aromatics, respectively, indicating a successful biodegradation. Community analysis showed that bacterial alfa diversity (Shannon Index), increased with the degradation of hydrocarbons, starting at an average value of 7.59 and ending at an average value of 9.38. Beta-diversity analysis, was performed using Bray-Curtis distances and PCoA ordination, where the two first principal components (PCs) explain the 17 and 14% of the observed variance, respectively. The results show that samples tend to cluster by sampling time instead of by Ecopile. This pattern is supported by the hierarchical clustering analysis, where most samples from the same timepoint clustered together. We used DSeq2 to determine the differential abundance of bacterial populations in Ecopiles at the beginning and the end of the treatment. While TPHs degraders are more abundant at the start of the experiment, these populations are substituted by bacterial populations typical of clean soils by the end of the biodegradation process. Similar results are found for the fungal community, indicating that the microbial community follows a succession along the process. This succession starts with a TPH degraders or tolerant enriched community, and finish with a microbial community typical of clean soils.</p></article>", "keywords": ["hydrocarbon", "bioremediation", "microbial succession", "microbiota", "Ecopile", " hydrocarbon", " bioremediation", " microbial succession", " microbiota", "Ecopile", "15. Life on land", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Microbiology", "6. Clean water", "QR1-502"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2023.1158130"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2023.1158130", "name": "item", "description": "10.3389/fmicb.2023.1158130", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2023.1158130"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-04-21T00:00:00Z"}}, {"id": "10.3390/microorganisms13040848", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:21:03Z", "type": "Journal Article", "created": "2025-04-10", "title": "Assessing Microbial Activity and Rhizoremediation in Hydrocarbon and Heavy Metal-Impacted Soil", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Rhizodegradation enhances pollutant degradation through plant\u2013microbe interactions in the rhizosphere. Plant roots provide a colonisation surface and root exudates that promote microbial abundance and activity, facilitating organic pollutant breakdown via direct microbial degradation and co-metabolism. This study assessed the rhizodegradation of weathered petroleum hydrocarbons (PHCs) in heavy metal co-contaminated soil in a microcosm-scale pot trial. Treatments included Sinapis alba, Lolium perenne, a L. perenne + Trifolium repens mix, and Cichorium intybus, alongside a non-planted control. After 14 weeks, PHC concentrations were analysed via gas chromatography, and rhizosphere microbial communities were characterised through sequencing. Sinapis alba achieved the highest PHC degradation (68%), significantly exceeding the non-planted control (p &lt; 0.05, Kruskal\u2013Wallis test). Hydrocarbon-degrading bacteria, including KCM-B-112, C1-B045, Hydrogenophaga, unclassified Saccharimonadales sp., and Pedobacter, were enriched in the rhizosphere, with the uncultured clade mle1-27 potentially contributing indirectly. Metals analysis of plant tissues showed that mustard could accumulate copper more than lead and zinc, despite higher concentrations of zinc and lead in the soil. These results highlight the potential of S. alba for rhizoremediation in PHC\u2013heavy metal co-contaminated soils.</p></article>", "keywords": ["petroleum hydrocarbons", "bioremediation", "QH301-705.5", "microbial communities", "phytoremediation", "Biology (General)", "heavy metals", "rhizodegradation", "Article"], "contacts": [{"organization": "Robert Conlon, David N. Dowling, Kieran J. Germaine,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.3390/microorganisms13040848"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms13040848", "name": "item", "description": "10.3390/microorganisms13040848", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms13040848"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-04-08T00:00:00Z"}}, {"id": "10.3390/microorganisms9040859", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:21:04Z", "type": "Journal Article", "created": "2021-04-19", "title": "Unraveling the metabolic potential of asgardarchaeota in a sediment from the mediterranean hydrocarbon-contaminated water basin mar piccolo (Taranto, italy)", "description": "<p>Increasing number of metagenome sequencing studies have proposed a central metabolic role of still understudied Archaeal members in natural and artificial ecosystems. However, their role in hydrocarbon cycling, particularly in the anaerobic biodegradation of aliphatic and aromatic hydrocarbons, is still mostly unknown in both marine and terrestrial environments. In this work, we focused our study on the metagenomic characterization of the archaeal community inhabiting the Mar Piccolo (Taranto, Italy, central Mediterranean) sediments heavily contaminated by petroleum hydrocarbons and polychlorinated biphenyls (PCB). Among metagenomic bins reconstructed from Mar Piccolo microbial community, we have identified members of the Asgardarchaeota superphylum that has been recently proposed to play a central role in hydrocarbon cycling in natural ecosystems under anoxic conditions. In particular, we found members affiliated with Thorarchaeota, Heimdallarchaeota, and Lokiarchaeota phyla and analyzed their genomic potential involved in central metabolism and hydrocarbon biodegradation. Metabolic prediction based on metagenomic analysis identified the malonyl-CoA and benzoyl-CoA routes as the pathways involved in aliphatic and aromatic biodegradation in these Asgardarchaeota members. This is the first study to give insight into the archaeal community functionality and connection to hydrocarbon degradation in marine sediment historically contaminated by hydrocarbons.</p>", "keywords": ["<i>Lokiarchaeota</i>", "0301 basic medicine", "0303 health sciences", "QH301-705.5", "<i>Heimdallarchaeota</i>", "aromatic hydrocarbons", "<i>Thorarchaeota</i>", "Asgardarchaeota", "metagenome", "Article", "6. Clean water", "03 medical and health sciences", "bioremediation", "13. Climate action", "Mar Piccolo", "aliphatic hydrocarbons", "14. Life underwater", "Biology (General)", "Asgardarchaeota; Mar Piccolo; aromatic hydrocarbons; aliphatic hydrocarbons; Thorarchaeota; Lokiarchaeota; Heimdallarchaeota; bioremediation; metagenome"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/9/4/859/pdf"}, {"href": "https://cris.unibo.it/bitstream/11585/819019/1/microorganisms-09-00859%20%282%29.pdf"}, {"href": "https://www.mdpi.com/2076-2607/9/4/859/pdf"}, {"href": "https://doi.org/10.3390/microorganisms9040859"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms9040859", "name": "item", "description": "10.3390/microorganisms9040859", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms9040859"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-04-16T00:00:00Z"}}, {"id": "10.3390/pr9030405", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:21:06Z", "type": "Journal Article", "created": "2021-02-25", "title": "Effects of the Feeding Solution Composition on a Reductive/Oxidative Sequential Bioelectrochemical Process for Perchloroethylene Removal", "description": "<p>Chlorinated aliphatic hydrocarbons (CAHs) are common groundwater contaminants due to their improper use in several industrial activities. Specialized microorganisms are able to perform the reductive dechlorination (RD) of high-chlorinated CAHs such as perchloroethylene (PCE), while the low-chlorinated ethenes such as vinyl chloride (VC) are more susceptible to oxidative mechanisms performed by aerobic dechlorinating microorganisms. Bioelectrochemical systems can be used as an effective strategy for the stimulation of both anaerobic and aerobic microbial dechlorination, i.e., a biocathode can be used as an electron donor to perform the RD, while a bioanode can provide the oxygen necessary for the aerobic dechlorination reaction. In this study, a sequential bioelectrochemical process constituted by two membrane-less microbial electrolysis cells connected in series has been, for the first time, operated with synthetic groundwater, also containing sulphate and nitrate, to simulate more realistic process conditions due to the possible establishment of competitive processes for the reducing power, with respect to previous research made with a PCE-contaminated mineral medium (with neither sulphate nor nitrate). The shift from mineral medium to synthetic groundwater showed the establishment of sulphate and nitrate reduction and caused the temporary decrease of the PCE removal efficiency from 100% to 85%. The analysis of the RD biomarkers (i.e., Dehalococcoides mccartyi 16S rRNA and tceA, bvcA, vcrA genes) confirmed the decrement of reductive dechlorination performances after the introduction of the synthetic groundwater, also characterized by a lower ionic strength and nutrients content. On the other hand, the system self-adapted the flowing current to the increased demand for the sulphate and nitrate reduction, so that reducing power was not in defect for the RD, although RD coulombic efficiency was less.</p>", "keywords": ["Reductive dechlorination", "oxidative dechlorination", "bioremediation", "reductive dechlorination", "01 natural sciences", "reductive dechlorination; oxidative dechlorination; bioelectrochemical systems; bioremediation", "bioelectrochemical systems", "6. Clean water", "0105 earth and related environmental sciences", "3. Good health"]}, "links": [{"href": "http://www.mdpi.com/2227-9717/9/3/405/pdf"}, {"href": "https://iris.uniroma1.it/bitstream/11573/1514918/1/Dell%e2%80%99Armi_Effects-feeding-solution_2021.pdf"}, {"href": "https://www.mdpi.com/2227-9717/9/3/405/pdf"}, {"href": "https://doi.org/10.3390/pr9030405"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Processes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/pr9030405", "name": "item", "description": "10.3390/pr9030405", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/pr9030405"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-24T00:00:00Z"}}, {"id": "10.3390/w11122579", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:21:13Z", "type": "Journal Article", "created": "2019-12-06", "title": "Reductive/Oxidative Sequential Bioelectrochemical Process for Perchloroethylene Removal", "description": "<p>An innovative bioelectrochemical reductive/oxidative sequential process was developed and tested on a laboratory scale to obtain the complete mineralization of perchloroethylene (PCE) in a synthetic medium. The sequential bioelectrochemical process consisted of two separate tubular bioelectrochemical reactors that adopted a novel reactor configuration, avoiding the use of an ion exchange membrane to separate the anodic and cathodic chamber and reducing the cost of the reactor. In the reductive reactor, a dechlorinating mixed inoculum received reducing power to perform the reductive dechlorination of perchloroethylene (PCE) through a cathode chamber, while the less chlorinated daughter products were removed in the oxidative reactor, which supported an aerobic dechlorinating culture through in situ electrochemical oxygen evolution. Preliminary fluid dynamics and electrochemical tests were performed to characterize both the reductive and oxidative reactors, which were electrically independent of each other, with each having its own counterelectrode. The first continuous-flow potentiostatic run with the reductive reactor (polarized at \uffe2\uff88\uff92450 mV vs SHE) resulted in obtaining 100% \uffc2\uffb1 1% removal efficiency of the influent PCE, while the oxidative reactor (polarized at +1.4 V vs SHE) oxidized the vinyl chloride and ethylene from the reductive reactor, with removal efficiencies of 100% \uffc2\uffb1 2% and 92% \uffc2\uffb1 1%, respectively.</p>", "keywords": ["bioelectrochemical systems; bioremediation; oxidative dechlorination; reductive dechlorination", "oxidative dechlorination", "bioremediation", "reductive dechlorination", "01 natural sciences", "7. Clean energy", "bioelectrochemical systems", "6. Clean water", "0105 earth and related environmental sciences", "3. Good health"]}, "links": [{"href": "http://www.mdpi.com/2073-4441/11/12/2579/pdf"}, {"href": "https://iris.uniroma1.it/bitstream/11573/1348028/1/Zeppilli_Reductive/oxidative-sequential_2019.pdf"}, {"href": "https://www.mdpi.com/2073-4441/11/12/2579/pdf"}, {"href": "https://doi.org/10.3390/w11122579"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/w11122579", "name": "item", "description": "10.3390/w11122579", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/w11122579"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-12-06T00:00:00Z"}}, {"id": "10486/701388", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:24:51Z", "type": "Journal Article", "created": "2021-03-15", "title": "Soil Microbiome Structure and Function in Ecopiles Used to Remediate Petroleum-Contaminated Soil", "description": "<p>The soil microbiome consists of a vast variety of microorganisms which contribute to essential ecosystem services including nutrient recycling, protecting soil structure, and pathogen suppression. Recalcitrant organic compounds present in soils contaminated with fuel oil can lead to a decrease in functional redundancy within soil microbiomes. Ecopiling is a passive bioremediation technique involving biostimulation of indigenous hydrocarbon degraders, bioaugmentation through inoculation with known petroleum-degrading consortia, and phytoremediation. The current study investigates the assemblage of soil microbial communities and pollutant-degrading potential in soil undergoing the Ecopiling process, through the amplicon marker gene and metagenomics analysis of the contaminated soil. The analysis of key community members including bacteria, fungi, and nematodes revealed a surprisingly diverse microbial community composition within the contaminated soil. The soil bacterial community was found to be dominated by Alphaproteobacteria (60\uffe2\uff80\uff9370%) with the most abundant genera such as Lysobacter, Dietzia, Pseudomonas, and Extensimonas. The fungal community consisted mainly of Ascomycota (50\uffe2\uff80\uff9370% relative abundance). Soil sequencing data allowed the identification of key enzymes involved in the biodegradation of hydrocarbons, providing a novel window into the function of individual bacterial groups in the Ecopile. Although the genus Lysobacter was identified as the most abundant bacterial genus (11\uffe2\uff80\uff9346%) in all of the contaminated soil samples, the metagenomic data were unable to confirm a role for this group in petrochemical degradation. Conversely, genera with relatively low abundance such as Dietzia (0.4\uffe2\uff80\uff939.0%), Pusillimonas (0.7\uffe2\uff80\uff932.3%), and Bradyrhizobium (0.8\uffe2\uff80\uff931.8%) did possess genes involved in aliphatic or aromatic compound degradation.</p", "keywords": ["0301 basic medicine", "2. Zero hunger", "metagenomics", "microbiome", "phytoremediation", "15. Life on land", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "6. Clean water", "Phytoremediation", "12. Responsible consumption", "Environmental sciences", "Ecopiling", "03 medical and health sciences", "bioremediation", "13. Climate action", "GE1-350", "Microbiome", "Metagenomics", "Bioremediation"]}, "links": [{"href": "https://doi.org/10486/701388"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Environmental%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10486/701388", "name": "item", "description": "10486/701388", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10486/701388"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-03-15T00:00:00Z"}}, {"id": "10259/9749", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:24:38Z", "type": "Journal Article", "created": "2024-12-01", "title": "Metal(loid) tolerance, accumulation, and phytoremediation potential of wetland macrophytes for multi-metal(loid)s polluted water.", "description": "<title>Abstract</title>         <p>Natural based solutions, notably constructed/artificial wetland treatment systems, rely heavily on identification and use of macrophytes with the ability to tolerate multiple contaminants and grow for an extended period to reduce contamination. The potential to tolerate and remediate metal(loid) contaminated groundwater from an industrial site located in Flanders (Belgium) was assessed for 10 wetland macrophytes (including <italic>Carex riparia, Cyperus longus, Cyperus rotundus, Iris pseudacorus, Juncus effusus, Lythrum salicaria, Menta aquatica, Phragmites australis, Scirpus holoschoenus,</italic> and <italic>Typha angustifolia</italic>). The experiment was conducted under static conditions, where plants were exposed to polluted acidic (pH~4)water, having high level of metal(loid)s for 15 days. Plant biomass, morphology, and metal uptake by roots and shoots were analysed every 5 days for all species. <italic>T. angustifolia</italic> and <italic>S. holoschoenus </italic>produced ~3 and ~1.1 times more dried biomass than the controls, respectively. For <italic>S. holoschoenus, P. australis,</italic> and <italic>T. angustifolia</italic>, no apparent morphological stress symptoms were observed, and plant heights were similar between control and plants exposed to polluted groundwater. Higher concentrations of all metal(loid)s were detected in the roots indicating a potential for phytostabilization of metal(loid)s below the water column. For <italic>J. effusus</italic> and <italic>T. angustifolia</italic>, Cd, Ni, and Zn accumulation was observed higher in the shoots. <italic>S. holoschoenus</italic>, <italic>P. australis,</italic> and <italic>T. angustifolia</italic> are proposed for restoration and phytostabilization strategies in natural and/or constructed wetland and aquatic ecosystems affected by metal(loid) inputs.</p>", "keywords": ["580", "570", "Constructed wetlands ; Metals/metabolism [MeSH] ; Groundwater ; Phytostabilization ; Wetlands [MeSH] ; Metals", " Heavy/metabolism [MeSH] ; Heavy metals ; Macrophytes ; Water Pollutants", " Chemical/metabolism [MeSH] ; Research Article ; Biodegradation", " Environmental [MeSH] ; Belgium [MeSH]", "Constructed wetlands", "15. Life on land", "Biorremediaci\u00f3n", "6. Clean water", "Macrophytes", "Agua-Contaminaci\u00f3n", "Biodegradation", " Environmental", "Heavy metals", "Water-Pollution", "Belgium", "Metals", "13. Climate action", "Wetlands", "Metals", " Heavy", "Phytostabilization", "Groundwater", "Bioremediation", "Water Pollutants", " Chemical", "Research Article"]}, "links": [{"href": "https://doi.org/10259/9749"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10259/9749", "name": "item", "description": "10259/9749", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10259/9749"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-12T00:00:00Z"}}, {"id": "10379/16163", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:24:48Z", "type": "Journal Article", "created": "2020-08-14", "title": "Simultaneous adsorption and biodegradation of trichloroethylene occurs in a biochar packed column treating contaminated landfill leachate", "description": "Trichloroethylene (TCE) is a human carcinogen that is commonly found in landfill leachate as a result of anthropogenic activities. Contaminated leachate plumes may be intercepted prior to reaching groundwater and treated in situ using permeable reactive barriers (PRB). This study used a packed column system containing herbal pomace and spruce biochar, previously shown to have TCE adsorptive capabilities, to investigate the feasibility of using pyrolysed waste as a fill material in a PRB. Influent containing raw or autoclaved landfill leachate was used to investigate the potential for environmental micro-organisms to establish a TCE-dechlorinating biofilm on the biochar, in order to prolong the operational life span of the system. TCE removal \u2265 99.7 was observed by both spruce and herbal pomace based biochars. No dichloroethylene (DCE) isomers were present in the column effluents, but cis-1,2 DCE was adsorbed to the biochar treating raw landfill leachate, indicating that dechlorination was occurring biologically in these columns. Known microbial species that are individually capable of complete dechlorination of TCE to ethene were not detected by 16S rRNA gene sequencing, but several species capable of partial TCE dechlorination (Desulfitobacterium spp., Sulfurospirillium spp. and Desulfuromonas spp) were present in the biofilms of the columns treating raw landfill leachate. These data demonstrate that biochar from waste material may be capable of supporting a dechlorinating biofilm to promote bioremediation of TCE.", "keywords": ["Permeable Reactive Barrier", "Waste reuse", "01 natural sciences", "6. Clean water", "Trichloroethylene", "12. Responsible consumption", "3. Good health", "Biochar", "Biodegradation", " Environmental", "13. Climate action", "Charcoal", "RNA", " Ribosomal", " 16S", "Humans", "Adsorption", "Water Pollutants", " Chemical", "Bioremediation", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10379/16163"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10379/16163", "name": "item", "description": "10379/16163", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10379/16163"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-01T00:00:00Z"}}, {"id": "10486/708477", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-25T16:24:51Z", "type": "Journal Article", "created": "2023-04-21", "title": "Field scale biodegradation of total petroleum hydrocarbons and soil restoration by Ecopiles: microbiological analysis of the process", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Ecopiling is a method for biodegradation of hydrocarbons in soils. It derives from Biopiles, but phytoremediation is added to biostimulation with nitrogen fertilization and bioaugmentation with local bacteria. We have constructed seven Ecopiles with soil heavily polluted with hydrocarbons in Carlow (Ireland). The aim of the study was to analyze changes in the microbial community during ecopiling. In the course of 18\u2009months of remediation, total petroleum hydrocarbons values decreased in 99 and 88% on average for aliphatics and aromatics, respectively, indicating a successful biodegradation. Community analysis showed that bacterial alfa diversity (Shannon Index), increased with the degradation of hydrocarbons, starting at an average value of 7.59 and ending at an average value of 9.38. Beta-diversity analysis, was performed using Bray-Curtis distances and PCoA ordination, where the two first principal components (PCs) explain the 17 and 14% of the observed variance, respectively. The results show that samples tend to cluster by sampling time instead of by Ecopile. This pattern is supported by the hierarchical clustering analysis, where most samples from the same timepoint clustered together. We used DSeq2 to determine the differential abundance of bacterial populations in Ecopiles at the beginning and the end of the treatment. While TPHs degraders are more abundant at the start of the experiment, these populations are substituted by bacterial populations typical of clean soils by the end of the biodegradation process. Similar results are found for the fungal community, indicating that the microbial community follows a succession along the process. This succession starts with a TPH degraders or tolerant enriched community, and finish with a microbial community typical of clean soils.</p></article>", "keywords": ["hydrocarbon", "bioremediation", "microbial succession", "microbiota", "Ecopile", "15. Life on land", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Microbiology", "6. Clean water", "QR1-502"]}, "links": [{"href": "https://doi.org/10486/708477"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10486/708477", "name": "item", "description": "10486/708477", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10486/708477"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-04-21T00:00:00Z"}}, {"id": "10486/717838", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:24:51Z", "type": "Journal Article", "created": "2024-09-28", "title": "Metagenomic analyses of a consortium for the bioremediation of hydrocarbons polluted soils", "description": "Abstract<p>A bacterial consortium was isolated from a soil in Noblejas (Toledo, Spain) with a long history of mixed hydrocarbons pollution, by enrichment cultivation. Serial cultures of hydrocarbons polluted soil samples were grown in a minimal medium using diesel (1\uffc2\uffa0mL/L) as the sole carbon and energy source. The bacterial composition of the Noblejas Consortium (NC) was determined by sequencing 16S rRNA gene amplicon libraries. The consortium contained around 50 amplicon sequence variants (ASVs) and the major populations belonged to the genera Pseudomonas, Enterobacter, Delftia, Stenotrophomonas, Achromobacter, Acinetobacter, Novosphingobium, Allorhizobium-Neorhizobium-Rhizobium, Ochrobactrum and Luteibacter. All other genera were below 1%. Metagenomic analysis of NC has shown a high abundance of genes encoding enzymes implicated in aliphatic and (poly) aromatic hydrocarbons degradation, and almost all pathways for hydrocarbon degradation are represented. Metagenomic analysis has also allowed the construction of metagenome assembled genomes (MAGs) for the major players of NC. Metatranscriptomic analysis has shown that several of the ASVs are implicated in hydrocarbon degradation, being Pseudomonas, Acinetobacter and Delftia the most active populations.</p", "keywords": ["metagenomics", "Bacterial consortium; Bioremediation; Metagenomics; Metatranscriptomics; Total petroleum hydrocarbons", "metatranscriptomics", "Bacterial consortium", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Microbiology", "QR1-502", "Total petroleum hydrocarbons", "total petroleum hydrocarbons", "bioremediation", "Original Article", "Metagenomics", "Bioremediation", "TP248.13-248.65", "Metatranscriptomics", "Biotechnology"]}, "links": [{"href": "https://doi.org/10486/717838"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/AMB%20Express", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10486/717838", "name": "item", "description": "10486/717838", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10486/717838"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-09-28T00:00:00Z"}}, {"id": "11245.1/7f8528e3-827f-4f02-91e5-87e1119aa5c1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:24:58Z", "type": "Journal Article", "created": "2023-01-18", "title": "Risk reductions during pyrene biotransformation and mobilization in a model plant-bacteria-biochar system", "description": "The productive application of motile microorganisms for degrading hydrophobic contaminants in soil is one of the most promising processes in modern remediation due to its sustainability and low cost. However, the incomplete biodegradation of the contaminants and the formation of the intermediary metabolites in the process may increase the toxicity in soil during bioremediation, and motile inoculants may mobilize the pollutants through biosorption. Therefore, controlling these factors should be a fundamental part of soil remediation approaches. The aim of this study was to evaluate the sources of risk associated with the cometabolism-based transformation of 14C-labeled pyrene by inoculated Pseudomonas putida G7 and identify ways to minimize risk. Our model scenario examined the increase in bioaccessibility to a distant source of contamination facilitated by sunflower (Helianthus annuus L.) roots. A biochar trap for mobilized pollutant metabolites and bacteria has also been employed. The experimental design consisted of pots filled with a layer of sand with 14C-labeled pyrene (88 mg kg-1) as a contamination focus located several centimeters from the inoculation point. Half of the pots included a biochar layer at the bottom. The pots were incubated in a greenhouse with sunflower plants and P. putida G7 bacteria. Pots with sunflower plants showed a higher biodegradation of pyrene, its mobilization as metabolites through the percolate and the roots, and bacterial mobilization toward the source of contamination, also resulting in increased pyrene transformation. In addition, the biochar layer efficiently reduced the concentrations of pyrene metabolites collected in the leachates. Therefore, the combination of plants, motile bacteria and biochar safely reduced the risk caused by the biological transformation of pyrene.", "keywords": ["Risk", "2. Zero hunger", "570", "Pyrenes", "Bacteria", "04 agricultural and veterinary sciences", "Plants", "01 natural sciences", "Polycyclic aromatic hydrocarbons", "6. Clean water", "Sunflower", "Soil", "Biodegradation", " Environmental", "13. Climate action", "Biodegradation", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Polycyclic Aromatic Hydrocarbons", "Bioremediation", "Biotransformation", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/11245.1/7f8528e3-827f-4f02-91e5-87e1119aa5c1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11245.1/7f8528e3-827f-4f02-91e5-87e1119aa5c1", "name": "item", "description": "11245.1/7f8528e3-827f-4f02-91e5-87e1119aa5c1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11245.1/7f8528e3-827f-4f02-91e5-87e1119aa5c1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-04-01T00:00:00Z"}}, {"id": "11584/303841", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:25:05Z", "type": "Journal Article", "created": "2020-11-30", "title": "Modelling the influence of soil properties on performance and bioremediation ability of a pile of soil microbial fuel cells", "description": "Abstract   Worldwide, intense industrial and agricultural activities pose serious issues of land contamination. Soil microbial fuel cells (SMFCs) have great potential as a low-cost, and self-powered solution to soil bioremediation, compatible with operations in remote areas. In this study, we propose a novel tubular SMFC design, in which a ceramic tube acts as the separator between the air-cathode and the anode, while providing structural support. No oxygen reduction reaction catalyst is used, and to reach depth, several SMFC units are piled together.  To assess the effect of both the system design and soil properties on performance, a mathematical model, calibrated with experimental data, is proposed, which accounts for chemical and (bio)electrochemical reactions, as well as for charge conservation and transport phenomena. The information generated provides useful indications on optimal design and operational conditions for SMFCs and a guide to effective scale-up strategies for their use in bioremediation.", "keywords": ["Soil microbial fuel cell", "/dk/atira/pure/subjectarea/asjc/1500/1500; name=General Chemical Engineering", "13. Climate action", "Soil microbial fuel cell; Bioremediation; Modelling; Hexachlorobenzene", "Hexachlorobenzene", "/dk/atira/pure/subjectarea/asjc/1600/1603; name=Electrochemistry", "01 natural sciences", "7. Clean energy", "Bioremediation", "Modelling", "6. Clean water", "0105 earth and related environmental sciences", "12. Responsible consumption"]}, "links": [{"href": "https://iris.unica.it/bitstream/11584/303841/5/pdf24_merged.pdf"}, {"href": "https://doi.org/11584/303841"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Electrochimica%20Acta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11584/303841", "name": "item", "description": "11584/303841", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11584/303841"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-01T00:00:00Z"}}, {"id": "2434/1027450", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-25T16:25:49Z", "type": "Journal Article", "created": "2024-02-02", "title": "Flavonoids influence key rhizocompetence traits for early root colonization and PCB degradation potential of Paraburkholderia xenovorans LB400", "description": "Introduction<p>Flavonoids are among the main plant root exudation components, and, in addition to their role in symbiosis, they can broadly affect the functionality of plant-associated microbes: in polluted environments, for instance, flavonoids can induce the expression of the enzymatic degradative machinery to clean-up soils from xenobiotics like polychlorinated biphenyls (PCBs). However, their involvement in root community recruitment and assembly involving non-symbiotic beneficial interactions remains understudied and may be crucial to sustain the holobiont fitness under PCB stress.</p>Methods<p>By using a set of model pure flavonoid molecules and a natural blend of root exudates (REs) with altered flavonoid composition produced by Arabidopsis mutant lines affected in flavonoid biosynthesis and abundance (null mutant tt4, flavonoid aglycones hyperproducer tt8, and flavonoid conjugates hyperaccumulator ttg), we investigated flavonoid contribution in stimulating rhizocompetence traits and the catabolic potential of the model bacterial strain for PCB degradation Paraburkholderia xenovorans LB400.</p>Results<p>Flavonoids influenced the traits involved in bacterial recruitment in the rhizoplane by improving chemotaxis and motility responses, by increasing biofilm formation and by promoting the growth and activation of the PCB-degradative pathway of strain LB400, being thus potentially exploited as carbon sources, stimulating factors and chemoattractant molecules. Indeed, early rhizoplane colonization was favored in plantlets of the tt8 Arabidopsis mutant and reduced in the ttg line. Bacterial growth was promoted by the REs of mutant lines tt4 and tt8 under control conditions and reduced upon PCB-18 stress, showing no significant differences compared with the WT and ttg, indicating that unidentified plant metabolites could be involved. PCB stress presumably altered the Arabidopsis root exudation profile, although a sudden \uffe2\uff80\uff9ccry-for-help\uffe2\uff80\uff9d response to recruit strain LB400 was excluded and flavonoids appeared not to be the main determinants. In the in vitro plant\uffe2\uff80\uff93microbe interaction assays, plant growth promotion and PCB resistance promoted by strain LB400 seemed to act through flavonoid-independent mechanisms without altering bacterial colonization efficiency and root adhesion pattern.</p>Discussions<p>This study further contributes to elucidate the vast array of functions provided by flavonoids in orchestrating the early events of PCB-degrading strain LB400 recruitment in the rhizosphere and to support the holobiont fitness by stimulating the catabolic machinery involved in xenobiotics decomposition and removal.</p", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "polychlorinated biphenyls", "plant-microbe interactions", "Plant culture", "Plant Science", "cry-for-help", "15. Life on land", "Rhizosphere; Beneficial bacteria; Bioremediation; Plant-microbe interactions; Polychlorinated biphenyls; Cry-for-help", "SB1-1110", "03 medical and health sciences", "bioremediation", "rhizosphere", "beneficial bacteria"]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/1027450/2/Ghitti%20et%20al.%2c%202024%20flavonoids%20Paraburkholderia.pdf"}, {"href": "https://doi.org/2434/1027450"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2434/1027450", "name": "item", "description": "2434/1027450", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2434/1027450"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-02-02T00:00:00Z"}}, {"id": "3136853395", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-25T16:26:19Z", "type": "Journal Article", "created": "2021-03-15", "title": "Soil Microbiome Structure and Function in Ecopiles Used to Remediate Petroleum-Contaminated Soil", "description": "<p>The soil microbiome consists of a vast variety of microorganisms which contribute to essential ecosystem services including nutrient recycling, protecting soil structure, and pathogen suppression. Recalcitrant organic compounds present in soils contaminated with fuel oil can lead to a decrease in functional redundancy within soil microbiomes. Ecopiling is a passive bioremediation technique involving biostimulation of indigenous hydrocarbon degraders, bioaugmentation through inoculation with known petroleum-degrading consortia, and phytoremediation. The current study investigates the assemblage of soil microbial communities and pollutant-degrading potential in soil undergoing the Ecopiling process, through the amplicon marker gene and metagenomics analysis of the contaminated soil. The analysis of key community members including bacteria, fungi, and nematodes revealed a surprisingly diverse microbial community composition within the contaminated soil. The soil bacterial community was found to be dominated by Alphaproteobacteria (60\uffe2\uff80\uff9370%) with the most abundant genera such as Lysobacter, Dietzia, Pseudomonas, and Extensimonas. The fungal community consisted mainly of Ascomycota (50\uffe2\uff80\uff9370% relative abundance). Soil sequencing data allowed the identification of key enzymes involved in the biodegradation of hydrocarbons, providing a novel window into the function of individual bacterial groups in the Ecopile. Although the genus Lysobacter was identified as the most abundant bacterial genus (11\uffe2\uff80\uff9346%) in all of the contaminated soil samples, the metagenomic data were unable to confirm a role for this group in petrochemical degradation. Conversely, genera with relatively low abundance such as Dietzia (0.4\uffe2\uff80\uff939.0%), Pusillimonas (0.7\uffe2\uff80\uff932.3%), and Bradyrhizobium (0.8\uffe2\uff80\uff931.8%) did possess genes involved in aliphatic or aromatic compound degradation.</p", "keywords": ["2. Zero hunger", "0301 basic medicine", "metagenomics", "microbiome", "phytoremediation", "15. Life on land", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "6. Clean water", "Phytoremediation", "12. Responsible consumption", "Environmental sciences", "Ecopiling", " bioremediation", " phytoremediation", " microbiome", " metagenomics", "Ecopiling", "03 medical and health sciences", "bioremediation", "13. Climate action", "GE1-350", "Microbiome", "Metagenomics", "Bioremediation"]}, "links": [{"href": "https://doi.org/3136853395"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Environmental%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "3136853395", "name": "item", "description": "3136853395", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/3136853395"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-03-15T00:00:00Z"}}, {"id": "50|od_______616::f7c80f8dd6460d4234bb717e3a1e29b0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:26:53Z", "type": "Journal Article", "title": "Application of a microbial consortium immobilized onto a biochar for the remediation of a polluted soil with hydrocarbons", "description": "Open AccessThe contamination of soils by hydrocarbons is a serious environmental problem both due to the persistence of this pollution and by its effects on even the most pristine areas of the planet. Hydrocarbon decontamination techniques entail a high cost, imply in many cases their off-site treatment, and entail environmental risks. In contrast, the use of microbiological techniques through the addition of microbial consortia with the capacity to degrade these contaminants, are alternatives for soil biorecovery that are gathering growing interest in the construction of biopiles or ecopiles. However, for an effective application of these biotechnological techniques, it is necessary to guarantee an effectiveness in the action of these microbial consortia, whose persistence in soil is generally low. In this work, the stabilization of a microbial consortium specialized in the degradation of hydrocarbons and isolated from the own soil, has been studied through its immobilization on two classes biochars obtained form apricot stones at pyrolysis temperatures of 450\u00b0 and 650\u00b0C, respectively, as well as the increase of its efficiency rate through the addition of rhamnolipids. The introduced microbial consortium achieved a degradation of 17% of the highest molecular weight hydrocarbons, an efficiency that was increased with the application of biochar and rhamnolipids up to 25% of their initial content.", "keywords": ["total petroleum hydrocarbons", "bioremediation", "biochar", "polluted soils", "rhamnolipids"], "contacts": [{"organization": "Curiel Alegre, Sandra, Velasco-Arroyo, Blanca, Mart\u00ednez, Andrea, Rumbo, Carlos, Tamayo-Ramos, Juan Antonio, Khan, Aqib A.H., Rad, Carlos, Barros, Roc\u00edo,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/50|od_______616::f7c80f8dd6460d4234bb717e3a1e29b0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Revista%20de%20Ci%C3%AAncias%20Agr%C3%A1rias", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "50|od_______616::f7c80f8dd6460d4234bb717e3a1e29b0", "name": "item", "description": "50|od_______616::f7c80f8dd6460d4234bb717e3a1e29b0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/50|od_______616::f7c80f8dd6460d4234bb717e3a1e29b0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-01T00:00:00Z"}}, {"id": "PMC12029208", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:28:09Z", "type": "Journal Article", "created": "2025-04-10", "title": "Assessing Microbial Activity and Rhizoremediation in Hydrocarbon and Heavy Metal-Impacted Soil", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Rhizodegradation enhances pollutant degradation through plant\u2013microbe interactions in the rhizosphere. Plant roots provide a colonisation surface and root exudates that promote microbial abundance and activity, facilitating organic pollutant breakdown via direct microbial degradation and co-metabolism. This study assessed the rhizodegradation of weathered petroleum hydrocarbons (PHCs) in heavy metal co-contaminated soil in a microcosm-scale pot trial. Treatments included Sinapis alba, Lolium perenne, a L. perenne + Trifolium repens mix, and Cichorium intybus, alongside a non-planted control. After 14 weeks, PHC concentrations were analysed via gas chromatography, and rhizosphere microbial communities were characterised through sequencing. Sinapis alba achieved the highest PHC degradation (68%), significantly exceeding the non-planted control (p &lt; 0.05, Kruskal\u2013Wallis test). Hydrocarbon-degrading bacteria, including KCM-B-112, C1-B045, Hydrogenophaga, unclassified Saccharimonadales sp., and Pedobacter, were enriched in the rhizosphere, with the uncultured clade mle1-27 potentially contributing indirectly. Metals analysis of plant tissues showed that mustard could accumulate copper more than lead and zinc, despite higher concentrations of zinc and lead in the soil. 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