{"type": "FeatureCollection", "features": [{"id": "10.1002/bbb.2656", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:55:00Z", "type": "Journal Article", "created": "2024-07-06", "title": "Feasibility of using phytoremediation biomass for sustainable biofuel production via thermochemical conversion", "description": "Abstract

This study explores a novel approach that combines soil recovery with biofuel production, presenting a strategy that addresses the increasing demand for biofuels while sidestepping the food\uffe2\uff80\uff93fuel debate. It also introduces an innovative method for recovering heavy metals from soils through their translocation into the solid product of the conversion process. Phytoremediation trials were conducted under real field conditions, and the thermochemical conversion of the harvested biomass was carried out at lab scale. Field trials took place in 2021\uffe2\uff80\uff932023 in Lithuania and Serbia. In Serbia, the contamination primarily involved heavy metals, whereas the Lithuanian site was predominantly contaminated with hydrocarbons from petroleum products. The harvested biomass underwent pretreatment and was then used as feedstock for conversion into high\uffe2\uff80\uff90energy carriers. The conversion products were evaluated for their potential to substitute fossil fuels. Finally, the value chain, encompassing key stakeholders and factors impacting the profitability of this approach, was established, and initial estimates were made regarding the size of individual cost components.Anaerobic digestion (AD) of protein-rich grass silage was performed in experimental two-stage two-phase biogas reactor systems at low vs. increased organic loading rates (OLRs) under mesophilic (37\uffe2\uff80\uff89\uffc2\uffb0C) and thermophilic (55\uffe2\uff80\uff89\uffc2\uffb0C) temperatures. To follow the adaptive response of the biomass-attached cellulolytic/hydrolytic biofilms at increasing ammonium/ammonia contents, genome-centered metagenomics and transcriptional profiling based on metagenome assembled genomes (MAGs) were conducted.

Results

In total, 78 bacterial and archaeal MAGs representing the most abundant members of the communities, and featuring defined quality criteria were selected and characterized in detail. Determination of MAG abundances under the tested conditions by mapping of the obtained metagenome sequence reads to the MAGs revealed that MAG abundance profiles were mainly shaped by the temperature but also by the OLR. However, the OLR effect was more pronounced for the mesophilic systems as compared to the thermophilic ones. In contrast, metatranscriptome mapping to MAGs subsequently normalized to MAG abundances showed that under thermophilic conditions, MAGs respond to increased OLRs by shifting their transcriptional activities mainly without adjusting their proliferation rates. This is a clear difference compared to the behavior of the microbiome under mesophilic conditions. Here, the response to increased OLRs involved adjusting of proliferation rates and corresponding transcriptional activities. The analysis led to the identification of MAGs positively responding to increased OLRs. The most outstanding MAGs in this regard, obviously well adapted to higher OLRs and/or associated conditions, were assigned to the order Clostridiales (Acetivibrio sp.) for the mesophilic biofilm and the orders Bacteroidales (Prevotella sp. and an unknown species), Lachnospirales (Herbinix sp. and Kineothrix sp.) and Clostridiales (Clostridium sp.) for the thermophilic biofilm. Genome-based metabolic reconstruction and transcriptional profiling revealed that positively responding MAGs mainly are involved in hydrolysis of grass silage, acidogenesis and / or\uffc2\uffa0acetogenesis.

Conclusions

An integrated -omics approach enabled the identification of new AD biofilm keystone species featuring outstanding performance under stress conditions such as increased OLRs. Genome-based knowledge on the metabolic potential and transcriptional activity of responsive microbiome members will contribute to the development of improved microbiological AD management strategies for biomethanation of renewable biomass.

", "keywords": ["Integrated -omics", "Bioconversion", "0301 basic medicine", "570", "Polyomics", "Integrated-omics", "Biogas", "Microbiology", "7. Clean energy", "03 medical and health sciences", "Anaerobic digestion", "GE1-350", "2. Zero hunger", "0303 health sciences", "Metagenome assembled genomes", "Microbial community structure", "15. Life on land", "QR1-502", "6. Clean water", "3. Good health", "Environmental sciences", "Metagenome assembled genomes", " Integrated -omics", " Polyomics", " Anaerobic digestion", " Biogas", " Bioconversion", " Microbial community structure", " Methane", " Metabolic activity", "13. Climate action", "Metabolic activity", "Methane", "Research Article"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1186/s40793-020-00354-x.pdf"}, {"href": "https://doi.org/10.1186/s40793-020-00354-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40793-020-00354-x", "name": "item", "description": "10.1186/s40793-020-00354-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40793-020-00354-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-02T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2007.03.012", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:57:41Z", "type": "Journal Article", "created": "2007-04-24", "title": "Soil Carbon Turnover And Sequestration In Native Subtropical Tree Plantations", "description": "Approximately 30% of global soil organic carbon (SOC) is stored in subtropical and tropical ecosystems but it is being rapidly lost due to continuous deforestation. Tree plantations are advocated as a C sink, however, little is known about rates of C turnover and sequestration into soil organic matter under subtropical and tropical tree plantations. We studied changes in SOC in a chronosequence of hoop pine (Araucaria cwunninghamii) plantations established on former rainforest sites in seasonally dry subtropical Australia. SOC, delta C-13, and light fraction organic C (LF C < 1.6 g cm(-3)) Were determined in plantations, secondary rainforest and pasture. We calculated loss of rainforest SOC after clearing for pasture using an isotope mixing model, and used the decay rate of rainforest-derived C to predict input of hoop pine-derived C into the soil. Total SOC stocks to 100 cm depth were significantly (P < 0.01) higher under rainforest (241 t ha(-1)) and pasture (254 t ha(-1)) compared to hoop pine (176-211 t ha(-1)). We calculated that SOC derived from hoop pine inputs ranged from 32% (25 year plantation) to 61% (63 year plantation) of total SOC in the 0-30 cm soil layer, but below 30 cm all C originated from rainforest. These results were compared to simulations made by the Century soil organic matter model. The Century model Simulations showed that lower C stocks under hoop pine plantations were due to reduced C inputs to the slow turnover C pool, such that this pool only recovers to within 45% of the original rainforest C pool after 63 years. This may indicate differences in soil C stabilization mechanisms under hoop pine plantations compared with rainforest and pasture. These results demonstrate that subtropical hoop pine plantations do not rapidly sequester SOC into long-term storage pools, and that alternative plantation systems may need to be investigated to achieve greater soil C sequestration. (c) 2007 Elsevier Ltd. All rights reserved.", "keywords": ["Araucaria", "C-13", "Soil Science", "Land-use Change", "Storage", "Puerto-rico", "Century model", "01 natural sciences", "C1", "light fraction carbon", "Pasture", "300103 Soil Chemistry", "Southern Queensland", "Rain-forest", "0105 earth and related environmental sciences", "tree plantations", "Organic-matter Dynamics", "770702 Land and water management", "04 agricultural and veterinary sciences", "15. Life on land", "Long-term Trends", "carbon sequestration", "soil organic carbon", "Forest Conversion", "Continuous Cultivation", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2007.03.012"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2007.03.012", "name": "item", "description": "10.1016/j.soilbio.2007.03.012", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2007.03.012"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-08-01T00:00:00Z"}}, {"id": "10.1111/j.1365-2389.2008.01059.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:59:34Z", "type": "Journal Article", "created": "2008-08-27", "title": "Influence Of Land Use (Savanna, Pasture,Eucalyptusplantations) On Soil Carbon And Nitrogen Stocks In Brazil", "description": "Summary

In Brazil, mostEucalyptusstands have been planted on Cerrado (shrubby savanna) or on Cerrado converted into pasture. Case studies are needed to assess the effect of such land use changes on soil fertility and C sequestration. In this study, the influence of Cerrado land development (pasture andEucalyptusplantations) on soil organic carbon (SOC) and nitrogen (SON) stocks were quantified in southern Brazil. Two contrasted silvicultural practices were also compared: 60\uffe2\uff80\uff83years of short\uffe2\uff80\uff90rotation silviculture (EUCSR) versus 60\uffe2\uff80\uff83years of continuous growth (EUCHF). C and N soil concentrations and bulk densities were measured and modelled for each vegetation type, and SOC and SON stocks were calculated down to a depth of 1\uffe2\uff80\uff83m by a continuous function.

Changes in SOC and SON stocks mainly occurred in the forest floor (no litter in pasture and up to 0.87\uffe2\uff80\uff83kg C\uffe2\uff80\uff83m\uffe2\uff88\uff922and 0.01\uffe2\uff80\uff83kg N\uffe2\uff80\uff83m\uffe2\uff88\uff922in EUCSR) and upper soil horizons. C and N stocks and their confidence intervals were greatly influenced by the methodology used to compute these layers. C/N ratio and13C analysis showed that down to a depth of 30\uffe2\uff80\uff83cm, the Cerrado organic matter was replaced by organic matter from newly introduced vegetation by as much as 75\uffe2\uff80\uff93100% for pasture and about 50% for EUCHF, poorer in N forEucalyptusstands (C/N larger than 18 forEucalyptusstands). Under pasture, 0\uffe2\uff80\uff9330\uffe2\uff80\uff83cm SON stocks (0.25\uffe2\uff80\uff83kg N\uffe2\uff80\uff83m\uffe2\uff88\uff922) were between 10 and 20% greater than those of the Cerrado (0.21\uffe2\uff80\uff83kg N\uffe2\uff80\uff83m\uffe2\uff88\uff922), partly due to soil compaction (limit bulk density at soil surface from 1.23 for the Cerrado to 1.34 for pasture). Land development on the Cerrado increased SOC stocks in the 0\uffe2\uff80\uff9330\uffe2\uff80\uff83cm layer by between 15 and 25% (from 2.99 (Cerrado) to 3.86 (EUCSR)\uffe2\uff80\uff83kg C\uffe2\uff80\uff83m\uffe2\uff88\uff922). When including litter layers, total 0\uffe2\uff80\uff9330\uffe2\uff80\uff83cm carbon stocks increased by 35% for EUCHF(4.50\uffe2\uff80\uff83kg C\uffe2\uff80\uff83m\uffe2\uff88\uff922) and 53% for EUCSR(5.08\uffe2\uff80\uff83kg C\uffe2\uff80\uff83m\uffe2\uff88\uff922), compared with the Cerrado (3.28\uffe2\uff80\uff83kg C\uffe2\uff80\uff83m\uffe2\uff88\uff922), independently of soil compaction.

", "keywords": ["P33 - Chimie et physique du sol", "sol", "http://aims.fao.org/aos/agrovoc/c_24420", "http://aims.fao.org/aos/agrovoc/c_7071", "http://aims.fao.org/aos/agrovoc/c_5192", "STOCKS ET FLUX", "stockage", "[SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study", "910", "ORGANIC-MATTER DYNAMICS", "utilisation des terres", "p\u00e2turages", "http://aims.fao.org/aos/agrovoc/c_7427", "MANAGEMENT", "http://aims.fao.org/aos/agrovoc/c_5626", "savane", "http://aims.fao.org/aos/agrovoc/c_1301", "[SDV.SA.SDS] Life Sciences [q-bio]/Agricultural sciences/Soil study", "http://aims.fao.org/aos/agrovoc/c_35657", "azote", "2. Zero hunger", "Eucalyptus", "340", "CONGO", "04 agricultural and veterinary sciences", "15. Life on land", "FOREST", "sylviculture", "K10 - Production foresti\u00e8re", "TREE PLANTATIONS", "CONVERSION", "http://aims.fao.org/aos/agrovoc/c_1070", "13. Climate action", "http://aims.fao.org/aos/agrovoc/c_4182", "AFFORESTATION", "http://aims.fao.org/aos/agrovoc/c_6825", "0401 agriculture", " forestry", " and fisheries", "EASTERN AUSTRALIA", "P01 - Conservation de la nature et ressources fonci\u00e8res", "carbone", "impact sur l'environnement", "plantations", "http://aims.fao.org/aos/agrovoc/c_7156", "http://aims.fao.org/aos/agrovoc/c_5990", "LEAF-LITTER", "STORAGE", "mati\u00e8re organique du sol", "http://aims.fao.org/aos/agrovoc/c_2683"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2389.2008.01059.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/European%20Journal%20of%20Soil%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2389.2008.01059.x", "name": "item", "description": "10.1111/j.1365-2389.2008.01059.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2389.2008.01059.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-09-15T00:00:00Z"}}, {"id": "21.11116/0000-0007-B312-A", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:06:04Z", "type": "Journal Article", "created": "2020-12-17", "title": "The Role of Petrimonas mucosa ING2-E5AT in Mesophilic Biogas Reactor Systems as Deduced from Multiomics Analyses", "description": "

Members of the genera Proteiniphilum and Petrimonas were speculated to represent indicators reflecting process instability within anaerobic digestion (AD) microbiomes. Therefore, Petrimonas mucosa ING2-E5AT was isolated from a biogas reactor sample and sequenced on the PacBio RSII and Illumina MiSeq sequencers. Phylogenetic classification positioned the strain ING2-E5AT in close proximity to Fermentimonas and Proteiniphilum species (family Dysgonomonadaceae). ING2-E5AT encodes a number of genes for glycosyl-hydrolyses (GH) which are organized in Polysaccharide Utilization Loci (PUL) comprising tandem susCD-like genes for a TonB-dependent outer-membrane transporter and a cell surface glycan-binding protein. Different GHs encoded in PUL are involved in pectin degradation, reflecting a pronounced specialization of the ING2-E5AT PUL systems regarding the decomposition of this polysaccharide. Genes encoding enzymes participating in amino acids fermentation were also identified. Fragment recruitments with the ING2-E5AT genome as a template and publicly available metagenomes of AD microbiomes revealed that Petrimonas species are present in 146 out of 257 datasets supporting their importance in AD microbiomes. Metatranscriptome analyses of AD microbiomes uncovered active sugar and amino acid fermentation pathways for Petrimonas species. Likewise, screening of metaproteome datasets demonstrated expression of the Petrimonas PUL-specific component SusC providing further evidence that PUL play a central role for the lifestyle of Petrimonas species.

", "keywords": ["Bioconversion", "anaerobic digestion", "0301 basic medicine", "2. Zero hunger", "bioconversion", "Biomethanation", "QH301-705.5", "570 Biologie", "polysaccharide utilization loci", "metabolic pathway reconstruction", "16. Peace & justice", "7. Clean energy", "Article", "660.6", "biomethanation", "03 medical and health sciences", "Metabolic pathway reconstruction", "Polysaccharide utilization loci", "Anaerobic digestion", "carbohydrate-active enzymes", "Carbohydrate-active enzymes", "ddc:570", "Biology (General)"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/8/12/2024/pdf"}, {"href": "https://www.mdpi.com/2076-2607/8/12/2024/pdf"}, {"href": "https://doi.org/21.11116/0000-0007-B312-A"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "21.11116/0000-0007-B312-A", "name": "item", "description": "21.11116/0000-0007-B312-A", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/21.11116/0000-0007-B312-A"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-17T00:00:00Z"}}, {"id": "10.3390/microorganisms8122024", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:01:46Z", "type": "Journal Article", "created": "2020-12-17", "title": "The Role of Petrimonas mucosa ING2-E5AT in Mesophilic Biogas Reactor Systems as Deduced from Multiomics Analyses", "description": "

Members of the genera Proteiniphilum and Petrimonas were speculated to represent indicators reflecting process instability within anaerobic digestion (AD) microbiomes. Therefore, Petrimonas mucosa ING2-E5AT was isolated from a biogas reactor sample and sequenced on the PacBio RSII and Illumina MiSeq sequencers. Phylogenetic classification positioned the strain ING2-E5AT in close proximity to Fermentimonas and Proteiniphilum species (family Dysgonomonadaceae). ING2-E5AT encodes a number of genes for glycosyl-hydrolyses (GH) which are organized in Polysaccharide Utilization Loci (PUL) comprising tandem susCD-like genes for a TonB-dependent outer-membrane transporter and a cell surface glycan-binding protein. Different GHs encoded in PUL are involved in pectin degradation, reflecting a pronounced specialization of the ING2-E5AT PUL systems regarding the decomposition of this polysaccharide. Genes encoding enzymes participating in amino acids fermentation were also identified. Fragment recruitments with the ING2-E5AT genome as a template and publicly available metagenomes of AD microbiomes revealed that Petrimonas species are present in 146 out of 257 datasets supporting their importance in AD microbiomes. Metatranscriptome analyses of AD microbiomes uncovered active sugar and amino acid fermentation pathways for Petrimonas species. Likewise, screening of metaproteome datasets demonstrated expression of the Petrimonas PUL-specific component SusC providing further evidence that PUL play a central role for the lifestyle of Petrimonas species.

", "keywords": ["Bioconversion", "anaerobic digestion", "0301 basic medicine", "2. Zero hunger", "bioconversion", "Biomethanation", "QH301-705.5", "570 Biologie", "polysaccharide utilization loci", "metabolic pathway reconstruction", "16. Peace & justice", "7. Clean energy", "Article", "660.6", "biomethanation", "03 medical and health sciences", "Metabolic pathway reconstruction", "Polysaccharide utilization loci", "carbohydrate-active enzymes; polysaccharide utilization loci; anaerobic digestion; biomethanation; metabolic pathway reconstruction; bioconversion", "Anaerobic digestion", "carbohydrate-active enzymes", "Carbohydrate-active enzymes", "ddc:570", "Biology (General)"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/8/12/2024/pdf"}, {"href": "https://www.mdpi.com/2076-2607/8/12/2024/pdf"}, {"href": "https://doi.org/10.3390/microorganisms8122024"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms8122024", "name": "item", "description": "10.3390/microorganisms8122024", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms8122024"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-17T00:00:00Z"}}, {"id": "10.3390/molecules29215126", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-31T07:01:47Z", "type": "Journal Article", "created": "2024-10-31", "title": "Effect of the Reactor Material on the Reforming of Primary Syngas", "description": "

Syngas, mostly hydrogen and carbon monoxide, has traditionally been produced from coal and natural gas, with biomass gasification later emerging as a renewable process. It is widely used in fuel synthesis through the Fischer\u2013Tropsch (FT) process, where the H2/CO ratio is crucial in determining product efficiency and quality. In this sense, this study aimed to reform an emulated syngas resulting from the supercritical water gasification of biomass, tailoring it to meet the H2/CO ratio required for FT synthesis. Conditions resembling dry reforming were applied, using temperatures from 600 to 950 \u00b0C and steel wool as a catalyst. Additionally, the effects of Inconel and stainless steel as reactor materials on syngas reforming were investigated. When Inconel was used, H2/CO ratios ranged between 1.04 and 1.84 with steel wool and 1.28 and 1.67 without. When comparing reactions without steel wool performed either in the Inconel or the stainless steel reactors, those using Inconel consistently outperformed the stainless steel ones, achieving CH4 and CO2 conversions up to 95% and 76%, respectively, versus 0% and 39% with stainless steel. It was concluded that the Inconel reactor exhibited catalytic properties due to its high nickel content and specific oxides.

", "keywords": ["carbon dioxide conversion", "QD241-441", "dry reforming", "Inconel catalytic activity", "methane conversion", "Organic chemistry", "syngas", "stainless steel", "Article"]}, "links": [{"href": "https://doi.org/10.3390/molecules29215126"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Molecules", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/molecules29215126", "name": "item", "description": "10.3390/molecules29215126", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/molecules29215126"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-10-30T00:00:00Z"}}, {"id": "10.5071/31steubce2023-5co.7.4", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-31T07:02:22Z", "type": "Journal Article", "title": "Development and Commissioning of an Innovative Biorefinery for the Conversion of Contaminated Biomass into High-quality Energy Carriers", "description": "Open AccessAs a part of the EU-project \u201cPhy2Climate\u201d an innovative biorefinery is developed and commissioned, which uses contaminated biomass harvested from the pilot sites to produce high quality products. The center of the biorefinery is comprised of an advanced intermediate based pyrolysis process called thermo-catalytic reforming to gain intermediate products like gas, bio-oil, an aqueous phase, and biochar. These intermediates are individually refined. In case of the TCR-gas a purification step is implemented before refinement to reduce the concentration of critical gas components like NH3 and H2S to below 50 ppm. The aqueous phase is purified using an electrochemical process while at the same time producing hydrogen. The purified TCR-gas and the hydrogen from the electrochemical purification is used in a Gas-to-Liquid plant with the aim to produce sustainable fuels through a Fischer-Tropsch-synthesis. The so produced liquid hydrocarbons are distilled to produce gasoline (EN 228) and diesel (EN 590). The bio-oil is also refined by distillation to be used as marine fuel (ISO 8217). The produced biochar is not further refined and directly assessed as a substitute for petroleum-based coke in the copper smelting industry.", "keywords": ["13. Climate action", "biorefinery", "", "11. Sustainability", "polluted soil", "innovative concepts", "Biomass", "thermochemical conversion", "7. Clean energy", "biofuels", "6. Clean water", "12. Responsible consumption"], "contacts": [{"organization": "Kick, C., Peetz, P., Apfelbacher, A., Meiller, M., Daschner, R., Hornung, A.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5071/31steubce2023-5co.7.4"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/31st%20European%20Biomass%20Conference%20and%20Exhibition%20-%20Proceedings", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5071/31steubce2023-5co.7.4", "name": "item", "description": "10.5071/31steubce2023-5co.7.4", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5071/31steubce2023-5co.7.4"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-01T00:00:00Z"}}, {"id": "10033/622632", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:05:04Z", "type": "Journal Article", "created": "2020-03-02", "title": "Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics", "description": "Abstract Background

Anaerobic digestion (AD) of protein-rich grass silage was performed in experimental two-stage two-phase biogas reactor systems at low vs. increased organic loading rates (OLRs) under mesophilic (37\uffe2\uff80\uff89\uffc2\uffb0C) and thermophilic (55\uffe2\uff80\uff89\uffc2\uffb0C) temperatures. To follow the adaptive response of the biomass-attached cellulolytic/hydrolytic biofilms at increasing ammonium/ammonia contents, genome-centered metagenomics and transcriptional profiling based on metagenome assembled genomes (MAGs) were conducted.

Results

In total, 78 bacterial and archaeal MAGs representing the most abundant members of the communities, and featuring defined quality criteria were selected and characterized in detail. Determination of MAG abundances under the tested conditions by mapping of the obtained metagenome sequence reads to the MAGs revealed that MAG abundance profiles were mainly shaped by the temperature but also by the OLR. However, the OLR effect was more pronounced for the mesophilic systems as compared to the thermophilic ones. In contrast, metatranscriptome mapping to MAGs subsequently normalized to MAG abundances showed that under thermophilic conditions, MAGs respond to increased OLRs by shifting their transcriptional activities mainly without adjusting their proliferation rates. This is a clear difference compared to the behavior of the microbiome under mesophilic conditions. Here, the response to increased OLRs involved adjusting of proliferation rates and corresponding transcriptional activities. The analysis led to the identification of MAGs positively responding to increased OLRs. The most outstanding MAGs in this regard, obviously well adapted to higher OLRs and/or associated conditions, were assigned to the order Clostridiales (Acetivibrio sp.) for the mesophilic biofilm and the orders Bacteroidales (Prevotella sp. and an unknown species), Lachnospirales (Herbinix sp. and Kineothrix sp.) and Clostridiales (Clostridium sp.) for the thermophilic biofilm. Genome-based metabolic reconstruction and transcriptional profiling revealed that positively responding MAGs mainly are involved in hydrolysis of grass silage, acidogenesis and / or\uffc2\uffa0acetogenesis.

Conclusions

An integrated -omics approach enabled the identification of new AD biofilm keystone species featuring outstanding performance under stress conditions such as increased OLRs. Genome-based knowledge on the metabolic potential and transcriptional activity of responsive microbiome members will contribute to the development of improved microbiological AD management strategies for biomethanation of renewable biomass.

", "keywords": ["Integrated -omics", "Bioconversion", "0301 basic medicine", "2. Zero hunger", "570", "0303 health sciences", "Polyomics", "Integrated-omics", "Metagenome assembled genomes", "Biogas", "Microbial community structure", "15. Life on land", "Microbiology", "7. Clean energy", "QR1-502", "6. Clean water", "3. Good health", "Environmental sciences", "03 medical and health sciences", "13. Climate action", "Anaerobic digestion", "Metabolic activity", "GE1-350", "Methane", "Research Article"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1186/s40793-020-00354-x.pdf"}, {"href": "https://doi.org/10033/622632"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiome", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10033/622632", "name": "item", "description": "10033/622632", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10033/622632"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-02T00:00:00Z"}}, {"id": "PMC11547698", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-31T07:08:46Z", "type": "Journal Article", "created": "2024-10-31", "title": "Effect of the Reactor Material on the Reforming of Primary Syngas", "description": "

Syngas, mostly hydrogen and carbon monoxide, has traditionally been produced from coal and natural gas, with biomass gasification later emerging as a renewable process. It is widely used in fuel synthesis through the Fischer\u2013Tropsch (FT) process, where the H2/CO ratio is crucial in determining product efficiency and quality. In this sense, this study aimed to reform an emulated syngas resulting from the supercritical water gasification of biomass, tailoring it to meet the H2/CO ratio required for FT synthesis. Conditions resembling dry reforming were applied, using temperatures from 600 to 950 \u00b0C and steel wool as a catalyst. Additionally, the effects of Inconel and stainless steel as reactor materials on syngas reforming were investigated. When Inconel was used, H2/CO ratios ranged between 1.04 and 1.84 with steel wool and 1.28 and 1.67 without. When comparing reactions without steel wool performed either in the Inconel or the stainless steel reactors, those using Inconel consistently outperformed the stainless steel ones, achieving CH4 and CO2 conversions up to 95% and 76%, respectively, versus 0% and 39% with stainless steel. It was concluded that the Inconel reactor exhibited catalytic properties due to its high nickel content and specific oxides.

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