{"type": "FeatureCollection", "features": [{"id": "10.5061/dryad.ms2np57", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:02:18Z", "type": "Dataset", "title": "Data from: Grazing enhances belowground carbon allocation, microbial biomass, and soil carbon in a subtropical grassland", "description": "unspecifiedLignin PhenolsData file with results from lignin phenol extraction of soil samples, and plant tissue end-members (i.e. shoots, rhizomes and roots of Bahiagrass from inside and outside grazing exclosure). The major families are summarized as v (vannilyl), c (cinnamyl) and s (syringyl). Column 'vsc' represents the sum of v,s, and c, standardized per 100 mg organic carbon, whereas 'vsc.sed' is standardized per 1 g of soil weight. 'adal.v' is the acid-aldehyde ratio of the vanillyl family.gcb_lignin.csvSoils EA/IRMSFile with results of elemental analysis and isotope ratio mass spectrometry. Note that plot number needs to be paired with treatment ('trt') to generate a unique ID. 'Back' column identifies whether data is from background survey of exclosures, or from pulse-chase plots.Soils_EAIRMS.csvPulse Chase Vegetation EA/IRMSData with results of EA/IRMS analysis on plant samples from pulse chase experiment. Note that plot number and treatment must be combined to generate a unique plot ID. Harvest identifies time post pulse (2 days, 7 days, or 32 days). Pool identifies whether it is shoots ('Ag Veg'), roots or rhizomes ('Rh'). 'Sub' represents replicated harvested swaths ('A' or 'B') per harvest date (for 2 day and 7 day only, hence 32 day is identified as AA). One sample was accidentally combined in the field and is identified with A/B. Biomass is reported only for the t = 0 initial harvests of aboveground material.PulseChase_VegDataALL.csvPulse Chase Vegetation MassContains dry weight biomass for all plant samples collected in pulse chase experiment. Note that treatment and plot number must be combined to generate unique plot ID. 'Sub' identifies replicate harvest swath collected at each harvest date (2 day and 7 day only, 32 day only had one swath and is thus identified as AA, as is the t=0 harvest of aboveground tissue immediately post pulse). 'Harvest' identifies time of harvest post pulse and is either 0 (immediately post pulse, aboveground tissue only), '2d' (2 day), '7d' (7 day), or '32day' (32 days). 'Pool' identifies the plant tissue and is either shoots ('Ag Veg'), roots ('Root') or rhizomes ('Rh'). Biomass is in grams. 'Standing dead' represents the senesced tissue sorted out of the sample prior to analysis, and is also in grams.PlantData_MassALL.csvPulse Chase Microbial DataMicrobial biomass and isotope ratio data. Note that treatment and plot number must be combined to generate a unique sample ID. Harvest indicates time since post pulse: '48' represents 2 days, '336' represents 7 days, and 4 represents 32 days. 'Sub' represents replicated harvest swath within a given harvest date (2 day and 7 day only). Column 'Rep' can be ignored. 'mgC/dry_mass_soil(g)' represents the carbon concentration of the extract, standardized per gram of soil extracted. 'd13c (permil, versus VPDB)' is the standard isotope delta 13C value, and 'Fumigated' identifies fumigated extracts (containing lysed microbial cell contents in addition to dissolved organic carbon, DOC) and 'unfumigated' extracts (which contain just DOC). Formulae for calculating microbial biomass and isotope enrichment are in the main paper, and are also explained in the open source code used to process and analyze data available at https://github.com/chwilson/GCB_2018).PulseChase_MicrobialData.csv", "keywords": ["2. Zero hunger", "belowground carbon allocation", "soil organic carbon", "microbial biomass", "Lignin Phenols", "Paspalum notatum", "Large Herbivore Grazing", "subtropical pasture", "15. Life on land"], "contacts": [{"organization": "Wilson, Chris H., Strickland, Michael S., Hutchings, Jack A., Bianchi, Thomas S., Flory, S. Luke,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.ms2np57"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.ms2np57", "name": "item", "description": "10.5061/dryad.ms2np57", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.ms2np57"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-02-13T00:00:00Z"}}, {"id": "10.1016/j.agrformet.2011.12.008", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:56:24Z", "type": "Journal Article", "created": "2012-01-29", "title": "Long- And Short-Term Precipitation Effects On Soil Co2 Efflux And Total Belowground Carbon Allocation", "description": "Abstract Soil CO 2 efflux ( E soil ), the main pathway of C movement from the biosphere to the atmosphere, is critical to the terrestrial C cycle but how precipitation and soil moisture influence E soil remains poorly understood. Here, we irrigated a longleaf pine wiregrass savanna for six years; this increased soil moisture by 41.2%. We tested how an altered precipitation regime affected total belowground carbon allocation (TBCA), root growth, soil carbon, and E soil . We used two methods to quantify E soil : daytime biweekly manual measurements and automated continuous measurements for one year. We hypothesized that the low-frequency manual method would miss both short- and long-term (i.e., subdaily to annual, respectively) effects of soil moisture on E soil while the high-frequency data from the automated method would allow the effects of soil moisture to be discerned. Root growth was significantly higher in irrigated plots, particularly at 0\u201320\u00a0cm depth. Irrigated annual E soil was significantly greater than that of the control when estimated with the continuous measurements but not when estimated from biweekly measurements. The difference in annual E soil estimates is likely due to (1) the delayed increase in E soil following irrigation pulses of soil moisture (i.e., variation that the biweekly manual measurements missed) and (2) the diel timing of biweekly manual measurements (they were completed early to mid-day before peak efflux). With irrigation, estimates of TBCA increased almost two-fold with automated measurements but only 36% with intermittent measurements. Relative to controls, irrigated treatments stored almost 2\u00a0Mg\u00a0C\u00a0ha \u22121 \u00a0year \u22121 more in soils and 0.26\u00a0Mg\u00a0C\u00a0ha \u22121 \u00a0year \u22121 more in roots. High-frequency measurements of E soil were essential to estimate total belowground carbon allocation. With irrigation, soil carbon pools were not at steady-state, so shifts in soil carbon storage must be considered in TBCA estimates.", "keywords": ["2. Zero hunger", "Root demography", "Carbon allocation", "Soil respiration", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "6. Clean water", "13. Climate action", "Climate change", "0401 agriculture", " forestry", " and fisheries", "Longleaf pine", "Irrigation", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.agrformet.2011.12.008"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agricultural%20and%20Forest%20Meteorology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agrformet.2011.12.008", "name": "item", "description": "10.1016/j.agrformet.2011.12.008", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agrformet.2011.12.008"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-04-01T00:00:00Z"}}, {"id": "10.1016/j.foreco.2022.120355", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:57:09Z", "type": "Journal Article", "created": "2022-06-22", "title": "Do mycorrhizal symbionts drive latitudinal trends in photosynthetic carbon use efficiency and carbon sequestration in boreal forests?", "description": "There is evidence that carbon fluxes and stocks decrease with increasing latitude in boreal forests, suggesting a reduction in carbon use efficiency. While vegetation and soil carbon dynamics have been widely studied, the empirical finding that ectomycorrhizal fungi (ECM) become more abundant towards the north has not been quantitatively linked to carbon use efficiency. We formulated a conceptual model of combined fine-root and ECM carbon use efficiency (CUE) as NPP/GPP (net primary production/gross primary production). For this, we included the mycorrhiza as gains in plant NPP but considered the extramatrical hyphae as well as exudates as losses. We quantified the carbon processes across a latitudinal gradient using published eco-physiological and morphological measurements from boreal coniferous forests. In parallel, we developed two CUE models using large-scale empirical measurements amended with established models. All models predicted similar latitudinal trends in vegetation CUE and net ecosystem production (NEP). CUE in the ECM model declined on average by 0.1 from latitude 60 to 70 with overall mean 0.390 +/- 0.037. NEP declined by 200 g m(-2) yr(-1) with mean 171 +/- 79.4 g m(-2) yr(-)(1). ECM had no significant effect on predicted soil carbon. Our findings suggest that ECM can use a significant proportion of the carbon assimilated by vegetation and hence be an important driver of the decline in CUE at higher latitudes. Our model suggests the quantitative contribution of ECM to soil carbon to be less important but any possible implications through litter quality remain to be assessed. The approach provides a simple proxy of ECM processes for regional C budget models and estimates.", "keywords": ["Soil C balance", "570", "550", "Forest Science", "hiilen kierto", "Carbon residence time", "Carbon use efficiency", "Forestry", "Carbon allocation", "hiilensidonta", "15. Life on land", "ta4112", "13. Climate action", "maaper\u00e4geologia", "Net ecosystem production", "Soil C:N ratio", "Mycorrhiza", "Model"]}, "links": [{"href": "https://pub.epsilon.slu.se/31150/1/makela-a-et-al-20230622.pdf"}, {"href": "https://doi.org/10.1016/j.foreco.2022.120355"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Forest%20Ecology%20and%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.foreco.2022.120355", "name": "item", "description": "10.1016/j.foreco.2022.120355", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.foreco.2022.120355"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-09-01T00:00:00Z"}}, {"id": "10.1016/j.foreco.2008.09.021", "type": "Feature", "geometry": null, "properties": {"license": "Restricted", "updated": "2026-05-31T06:57:05Z", "type": "Journal Article", "created": "2008-10-27", "title": "Carbon Dynamics Of A Ponderosa Pine Plantation Following A Thinning Treatment In The Northern Sierra Nevada", "description": "We investigated the impacts of a thinning prescription commonly used to reduce fuel loads in mature ponderosa pine forests of the northern Sierra Nevada Mountains on the distribution of carbon among functionally distinct pools, and on the component fluxes of net ecosystem production (NEP). The biomass of wood, foliage, and roots was highest in the unthinned control stands, lowest in stands measured 3 years after thinning, and intermediate in the stands measured 16 years following the same prescription. While total wood net primary production (NPP) followed the same pattern as biomass across treatments, an apparent doubling of shrub foliage NPP in the 3 years following overstory thinning reduced the impacts of thinning on total foliage NPP. Similarly, reductions in coarse root NPP associated with tree removal were largely offset by increases in fine root production. Compensatory NPP by shrubs and fine roots increased the light use efficiency of thinned stands 60% over that of unthinned plots. Both soil respiration and the decomposition of aboveground dead wood appeared conserved across all treatments. Results suggest that when modeling the influence fuel reduction treatments on regional carbon dynamics, it may be necessary to consider the compensatory responses of understory vegetation as such shifts in growth form can effect meaningful changes in the capture and allocation of carbon in the ecosystem.", "keywords": ["0106 biological sciences", "13. Climate action", "thinning; carbon allocation; net primary production", "15. Life on land", "01 natural sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://air.uniud.it/bitstream/11390/691284/1/Campbell_et_al_2009.pdf"}, {"href": "https://doi.org/10.1016/j.foreco.2008.09.021"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Forest%20Ecology%20and%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.foreco.2008.09.021", "name": "item", "description": "10.1016/j.foreco.2008.09.021", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.foreco.2008.09.021"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-01-01T00:00:00Z"}}, {"id": "10138/570094", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:05:07Z", "type": "Journal Article", "created": "2022-06-22", "title": "Do mycorrhizal symbionts drive latitudinal trends in photosynthetic carbon use efficiency and carbon sequestration in boreal forests?", "description": "There is evidence that carbon fluxes and stocks decrease with increasing latitude in boreal forests, suggesting a reduction in carbon use efficiency. While vegetation and soil carbon dynamics have been widely studied, the empirical finding that ectomycorrhizal fungi (ECM) become more abundant towards the north has not been quantitatively linked to carbon use efficiency. We formulated a conceptual model of combined fine-root and ECM carbon use efficiency (CUE) as NPP/GPP (net primary production/gross primary production). For this, we included the mycorrhiza as gains in plant NPP but considered the extramatrical hyphae as well as exudates as losses. We quantified the carbon processes across a latitudinal gradient using published eco-physiological and morphological measurements from boreal coniferous forests. In parallel, we developed two CUE models using large-scale empirical measurements amended with established models. All models predicted similar latitudinal trends in vegetation CUE and net ecosystem production (NEP). CUE in the ECM model declined on average by 0.1 from latitude 60 to 70 with overall mean 0.390 +/- 0.037. NEP declined by 200 g m(-2) yr(-1) with mean 171 +/- 79.4 g m(-2) yr(-)(1). ECM had no significant effect on predicted soil carbon. Our findings suggest that ECM can use a significant proportion of the carbon assimilated by vegetation and hence be an important driver of the decline in CUE at higher latitudes. Our model suggests the quantitative contribution of ECM to soil carbon to be less important but any possible implications through litter quality remain to be assessed. The approach provides a simple proxy of ECM processes for regional C budget models and estimates.", "keywords": ["Soil C balance", "570", "550", "Forest Science", "hiilen kierto", "Carbon residence time", "Carbon use efficiency", "Forestry", "Carbon allocation", "hiilensidonta", "15. Life on land", "ta4112", "13. Climate action", "maaper\u00e4geologia", "Net ecosystem production", "Soil C:N ratio", "Mycorrhiza", "Model"]}, "links": [{"href": "https://pub.epsilon.slu.se/31150/1/makela-a-et-al-20230622.pdf"}, {"href": "https://doi.org/10138/570094"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Forest%20Ecology%20and%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10138/570094", "name": "item", "description": "10138/570094", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10138/570094"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-09-01T00:00:00Z"}}, {"id": "10.1038/s41467-019-08348-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:58:29Z", "type": "Journal Article", "created": "2019-02-14", "title": "Decadal biomass increment in early secondary succession woody ecosystems is increased by CO2 enrichment", "description": "Abstract

Increasing atmospheric CO2 stimulates photosynthesis which can increase net primary production (NPP), but at longer timescales may not necessarily increase plant biomass. Here we analyse the four decade-long CO2-enrichment experiments in woody ecosystems that measured total NPP and biomass. CO2 enrichment increased biomass increment by 1.05\uffe2\uff80\uff89\uffc2\uffb1\uffe2\uff80\uff890.26\uffe2\uff80\uff89kg\uffe2\uff80\uff89C\uffe2\uff80\uff89m\uffe2\uff88\uff922 over a full decade, a 29.1\uffe2\uff80\uff89\uffc2\uffb1\uffe2\uff80\uff8911.7% stimulation of biomass gain in these early-secondary-succession temperate ecosystems. This response is predictable by combining the CO2 response of NPP (0.16\uffe2\uff80\uff89\uffc2\uffb1\uffe2\uff80\uff890.03\uffe2\uff80\uff89kg\uffe2\uff80\uff89C\uffe2\uff80\uff89m\uffe2\uff88\uff922\uffe2\uff80\uff89y\uffe2\uff88\uff921) and the CO2-independent, linear slope between biomass increment and cumulative NPP (0.55\uffe2\uff80\uff89\uffc2\uffb1\uffe2\uff80\uff890.17). An ensemble of terrestrial ecosystem models fail to predict both terms correctly. Allocation to wood was a driver of across-site, and across-model, response variability and together with CO2-independence of biomass retention highlights the value of understanding drivers of wood allocation under ambient conditions to\uffc2\uffa0correctly interpret\uffc2\uffa0and predict CO2 responses.

", "keywords": ["[SDE] Environmental Sciences", "0106 biological sciences", "0301 basic medicine", "TREE MORTALITY", "550", "Climate", "Plant Biology", "Biochemistry", "01 natural sciences", "Trees", "atmospheric carbon dioxide", "ddc:550", "Biomass", "Photosynthesis", "Ecology", "Q", "FOREST PRODUCTIVITY", "Forestry", "Biological Sciences", "woody", "decadal biomass", "Wood", "[SDE]Environmental Sciences", "GROWTH", "ecosystems", "CARBON ALLOCATION", "570", "Science", "Biophysics", "333", "SWEETGUM PLANTATION", "Article", "03 medical and health sciences", "XXXXXX - Unknown", "forest ecology", "plant biomass", "Biochemistry", " Biophysics", " and Structural Biology", "Ecosystem", "photosynthesis", "Carbon Dioxide", "15. Life on land", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "NITROGEN", "CLIMATE", "13. Climate action", "and Structural Biology", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology", "ELEVATED CO2", "SOIL CARBON", "RESPONSES"]}, "links": [{"href": "https://www.nature.com/articles/s41467-019-08348-1.pdf"}, {"href": "https://arrow.tudublin.ie/context/scschbioart/article/1214/viewcontent/nature.pdf"}, {"href": "https://escholarship.org/content/qt5m5806sh/qt5m5806sh.pdf"}, {"href": "https://doi.org/10.1038/s41467-019-08348-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-019-08348-1", "name": "item", "description": "10.1038/s41467-019-08348-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-019-08348-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-14T00:00:00Z"}}, {"id": "10.1093/treephys/tpad135", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:59:11Z", "type": "Journal Article", "created": "2023-11-09", "title": "Carbon dynamics in long-term starving poplar trees\u2014the importance of older carbohydrates and a shift to lipids during survival", "description": "Abstract

Carbon (C) assimilation can be severely impaired during periods of environmental stress, like drought or defoliation, making trees heavily dependent on the use of C reserve pools for survival; yet, the dynamics of reserve use during periods of reduced C supply are still poorly understood. We used stem girdling in mature poplar trees (Populus tremula L. hybrids), a lipid-storing species, to permanently interrupt the phloem C transport and induced C shortage in the isolated stem section below the girdle and monitored metabolic activity during three campaigns in the growing seasons of 2018, 2019 and 2021. We measured respiratory fluxes (CO2 and O2), non-structural carbon concentration, the respiratory substrate (based on isotopic analysis and CO2/O2 ratio) and the age of the respiratory substrate (based on radiocarbon analysis). Our study shows that poplar trees can survive long periods of reduced C supply from the canopy by switching in metabolism from recent carbohydrates to older storage pools with a potential mixture of respiratory substrates, including lipids. This mechanism of stress resilience can explain why tree decline may take many years before death occurs.

Drought periods are projected to become more severe and more frequent in many European regions. While effects of single strong droughts on plant and microbial carbon (C) dynamics have been studied in some detail, impacts of recurrent drought events are still little understood.

We tested whether the legacy of extreme experimental drought affects responses of plant and microbial C and nitrogen (N) turnover to further drought and rewetting. In a mountain grassland, we conducted a 13C pulse\uffe2\uff80\uff90chase experiment during a naturally occurring drought and rewetting event in plots previously exposed to experimental droughts and in ambient controls (AC). After labelling, we traced 13C below\uffe2\uff80\uff90ground allocation and incorporation into soil microbes using phospholipid fatty acid biomarkers.

Drought history (DH) had no effects on the standing shoot and fine root plant biomass. However, plants with experimental DH displayed decreased shoot N concentrations and increased fine root N concentrations relative to those in AC. During the natural drought, plants with DH assimilated and allocated less 13C below\uffe2\uff80\uff90ground; moreover, fine root respiration was reduced and not fuelled by fresh C compared to plants in AC.

Regardless of DH, microbial biomass remained stable during natural drought and rewetting. Although microbial communities initially differed in their composition between soils with and without DH, they responded to the natural drought and rewetting in a similar way: gram\uffe2\uff80\uff90positive bacteria increased, while fungal and gram\uffe2\uff80\uff90negative bacteria remained stable. In soils with DH, a strongly reduced uptake of recent plant\uffe2\uff80\uff90derived 13C in microbial biomarkers was observed during the natural drought, pointing to a smaller fraction of active microbes or to a microbial community that is less dependent on plant C.

Synthesis. Drought history can induce changes in above\uffe2\uff80\uff90 vs. below\uffe2\uff80\uff90ground plant N concentrations and affect the response of plant C turnover to further droughts and rewetting by decreasing plant C uptake and below\uffe2\uff80\uff90ground allocation. DH does not affect the responses of the microbial community to further droughts and rewetting, but alters microbial functioning, particularly the turnover of recent plant\uffe2\uff80\uff90derived carbon, during and after further drought periods.

", "keywords": ["0301 basic medicine", "plant-soil (below-ground) interactions", "NITROGEN TURNOVER", "Biomass Allocation", "microbial community composition", "Negibacteria", "drought", "phospholipid fatty acid", "nitrogen", "Microbial community composition", "Plant\u2013Soil (Below\u2010ground) Interactions", "Recovery", "ROOT RESPIRATION", "Plant-soil (below-ground) interactions", "CLIMATE EXTREMES", "C pulse labelling", "Below-ground carbon allocation", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "SOIL INTERACTIONS", "below-ground carbon allocation", "C-13 pulse labelling", "Grassland", "6. Clean water", "Europe", "Phospholipid", "ORGANIC-MATTER", "Mountain Region", "Posibacteria", "DIOXIDE PULSES", "Phospholipid fatty acid", "106022 Microbiology", "Root/shoot Ratio", "Belowground Biomass", "Ecosystem Resilience", "Nitrogen", "Microbial Community", "Carbon Isotope", "Soil-vegetation Interaction", "recovery", "SUMMER DROUGHT", "03 medical and health sciences", "Rewetting", "Community Composition", "plant\u2013soil (below-ground) interactions", "WATER-STRESS", "resilience", "Drought", "Resilience", "RESILIENCE", "15. Life on land", "Turnover", "Microbial Activity", "13. Climate action", "Fatty Acid", "RESPONSES"]}, "links": [{"href": "https://doi.org/10.1111/1365-2745.12593"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1365-2745.12593", "name": "item", "description": "10.1111/1365-2745.12593", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1365-2745.12593"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-05-24T00:00:00Z"}}, {"id": "10.1111/1365-2745.14136", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:59:21Z", "type": "Journal Article", "created": "2023-06-08", "title": "Drought intensity alters productivity, carbon allocation and plant nitrogen uptake in fast versus slow grassland communities", "description": "Abstract

Grasslands face more frequent and extreme droughts; yet, their responses to increasing drought intensity are poorly understood. Increasing drought intensity likely triggers abrupt shifts (thresholds) in grassland ecosystem functioning which can implicate recovery trajectories.

Here, we determined how drought intensity affects plant productivity, and plant\uffe2\uff80\uff93soil carbon (C) and nitrogen (N) cycling. We exposed model grassland plant communities with contrasting resource acquisition strategies (a fast\uffe2\uff80\uff90 vs a slow\uffe2\uff80\uff90strategy plant community), to a gradient of drought intensity. The drought gradient ranged from well\uffe2\uff80\uff90watered to severely water\uffe2\uff80\uff90limited conditions. We identified thresholds of plant community productivity (above\uffe2\uff80\uff90ground biomass) at peak drought and 2\uffe2\uff80\uff89months after re\uffe2\uff80\uff90wetting, and measured net ecosystem exchange and ecosystem respiration of C\uffc2\uffa0throughout the drought and recovery phases. At peak drought and 1\uffe2\uff80\uff89week after re\uffe2\uff80\uff90wetting, we traced recently acquired C from plants to the soil and into microbial biomass and fatty acids using 13C pulse labelling, and measured plant and soil N.

At peak drought, slow\uffe2\uff80\uff90strategy plant communities were more drought resistant than fast\uffe2\uff80\uff90strategy communities, as the threshold in plant productivity occurred at a higher drought intensity for the slow\uffe2\uff80\uff90 than the fast\uffe2\uff80\uff90strategy community. Shortly after re\uffe2\uff80\uff90wetting, microbial uptake of recent plant\uffe2\uff80\uff90assimilated C increased with increasing past drought intensity, coinciding with an increase in soil N availability and leaf N. Threshold responses to drought intensity at peak drought translated into non\uffe2\uff80\uff90linear recovery responses, with greater compensatory growth in the fast\uffe2\uff80\uff90strategy community. At peak drought, increasing drought intensity reduced C uptake and increased relative C partitioning to leaves and microbial biomass. Upon re\uffe2\uff80\uff90wetting, plant community strategy mediated drought intensity effects on plant and soil C and N dynamics and plant recovery trajectories. The fast\uffe2\uff80\uff90strategy community recovered quickly, with higher leaf N than the slow community, while the slow community increased C allocation to microbial biomass.

Synthesis. Our findings highlight that C and N dynamics in the plant\uffe2\uff80\uff93soil system display non\uffe2\uff80\uff90linear responses to increasing drought intensity both during and after drought, which has implications for plant community recovery trajectories.

In most natural ecosystems a significant portion of carbon fixed through photosynthesis is allocated to the production and maintenance of fine roots, the ephemeral portion of the root system that absorbs growth\uffe2\uff80\uff90limiting moisture and nutrients. In turn, senescence of fine roots can be the greatest source of C input to forest soils. Consequently, important questions in ecology entail the extent to which increasing atmospheric CO2may alter the allocation of carbon to, and demography of, fine roots. Using microvideo and image analysis technology, we demonstrate that elevated atmospheric CO2increases the rates of both fine root production and mortality. Rates of root mortality also increased substantially as soil nitrogen availability increased, regardless of CO2concentration. Nitrogen greatly influenced the proportional allocation of carbon to leaves vs. fine roots. The amount of available nitrogen in the soil appears to be the most important factor regulating fine root demography inPopulustrees.

", "keywords": ["0106 biological sciences", "2. Zero hunger", "Nitrogen", "Science", "Atmospheric CO 2", "Natural Resources and Environment", "04 agricultural and veterinary sciences", "15. Life on land", "Roots", "Turnover", "Global Warming", "01 natural sciences", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Carbon Allocation"]}, "links": [{"href": "https://doi.org/10.1111/j.1469-8137.1995.tb03025.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1469-8137.1995.tb03025.x", "name": "item", "description": "10.1111/j.1469-8137.1995.tb03025.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1469-8137.1995.tb03025.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1995-04-01T00:00:00Z"}}, {"id": "10.1111/j.1469-8137.2008.02564.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:59:44Z", "type": "Journal Article", "created": "2008-07-14", "title": "Soil Respiration, Root Biomass, And Root Turnover Following Long-Term Exposure Of Northern Forests To Elevated Atmospheric Co2and Tropospheric O3", "description": "The Rhinelander free-air CO(2) enrichment (FACE) experiment is designed to understand ecosystem response to elevated atmospheric carbon dioxide (+CO(2)) and elevated tropospheric ozone (+O(3)). The objectives of this study were: to understand how soil respiration responded to the experimental treatments; to determine whether fine-root biomass was correlated to rates of soil respiration; and to measure rates of fine-root turnover in aspen (Populus tremuloides) forests and determine whether root turnover might be driving patterns in soil respiration. Soil respiration was measured, root biomass was determined, and estimates of root production, mortality and biomass turnover were made. Soil respiration was greatest in the +CO(2) and +CO(2) +O(3) treatments across all three plant communities. Soil respiration was correlated with increases in fine-root biomass. In the aspen community, annual fine-root production and mortality (g m(-2)) were positively affected by +O(3). After 10 yr of exposure, +CO(2) +O(3)-induced increases in belowground carbon allocation suggest that the positive effects of elevated CO(2) on belowground net primary productivity (NPP) may not be offset by negative effects of O(3). For the aspen community, fine-root biomass is actually stimulated by +O(3), and especially +CO(2) +O(3).", "keywords": ["Greenhouse Effect", "2. Zero hunger", "Atmosphere", "Climate Change", "Science", "Natural Resources and Environment", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Plant Roots", "Trees", "Soil", "Ozone", "Populus", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Global Change", "Biomass", "Ozone (O 3 )", "Ecosystem", "Carbon Allocation", "Carbon Dioxide (CO 2 )", "Fine Roots"]}, "links": [{"href": "https://doi.org/10.1111/j.1469-8137.2008.02564.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1469-8137.2008.02564.x", "name": "item", "description": "10.1111/j.1469-8137.2008.02564.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1469-8137.2008.02564.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-09-02T00:00:00Z"}}, {"id": "10.1111/nph.12569", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T06:59:52Z", "type": "Journal Article", "created": "2013-10-31", "title": "Experimental Drought Reduces The Transfer Of Recently Fixed Plant Carbon To Soil Microbes And Alters The Bacterial Community Composition In A Mountain Meadow", "description": "Summary

Drought affects plants and soil microorganisms, but it is still not clear how it alters the carbon (C) transfer at the plant\uffe2\uff80\uff93microbial interface. Here, we tested direct and indirect effects of drought on soil microbes and microbial turnover of recent plant\uffe2\uff80\uff90derived C in a mountain meadow.

Microbial community composition was assessed using phospholipid fatty acids (PLFAs); the allocation of recent plant\uffe2\uff80\uff90derived C to microbial groups was analysed by pulse\uffe2\uff80\uff90labelling of canopy sections with 13CO2 and the subsequent tracing of the label into microbial PLFAs.

Microbial biomass was significantly higher in plots exposed to a severe experimental drought. In addition, drought induced a shift of the microbial community composition, mainly driven by an increase of Gram\uffe2\uff80\uff90positive bacteria. Drought reduced belowground C allocation, but not the transfer of recently plant\uffe2\uff80\uff90assimilated C to fungi, and in particular reduced tracer uptake by bacteria. This was accompanied by an increase of 13C in the extractable organic C pool during drought, which was even more pronounced after plots were mown.

We conclude that drought weakened the link between plant and bacterial, but not fungal, C turnover, and facilitated the growth of potentially slow\uffe2\uff80\uff90growing, drought\uffe2\uff80\uff90adapted soil microbes, such as Gram\uffe2\uff80\uff90positive bacteria.

", "keywords": ["Time Factors", "Nitrogen", "Mowing", "Mountain grassland", "Carbon Cycle", "Microbial community composition", "Soil", "Biomass", "Ecosystem", "Soil Microbiology", "2. Zero hunger", "106022 Mikrobiologie", "Carbon Isotopes", "Drought", "Research", "Microbiota", "Water", "Carbon allocation", "Microclimate", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon", "6. Clean water", "Droughts", "C pulse-labelling", "13. Climate action", "Austria", "Phospholipid fatty acids", "106022 Microbiology", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.1111/nph.12569"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.12569", "name": "item", "description": "10.1111/nph.12569", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.12569"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-10-31T00:00:00Z"}}, {"id": "10.3389/fmicb.2019.00168", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:01:31Z", "type": "Journal Article", "created": "2019-02-26", "title": "Rapid Transfer of Plant Photosynthates to Soil Bacteria via Ectomycorrhizal Hyphae and Its Interaction With Nitrogen Availability", "description": "Plant roots release recent photosynthates into the rhizosphere, accelerating decomposition of organic matter by saprotrophic soil microbes ('rhizosphere priming effect') which consequently increases nutrient availability for plants. However, about 90% of all higher plant species are mycorrhizal, transferring a significant fraction of their photosynthates directly to their fungal partners. Whether mycorrhizal fungi pass on plant-derived carbon (C) to bacteria in root-distant soil areas, i.e., incite a 'hyphosphere priming effect,' is not known. Experimental evidence for C transfer from mycorrhizal hyphae to soil bacteria is limited, especially for ectomycorrhizal systems. As ectomycorrhizal fungi possess enzymatic capabilities to degrade organic matter themselves, it remains unclear whether they cooperate with soil bacteria by providing photosynthates, or compete for available nutrients. To investigate a possible C transfer from ectomycorrhizal hyphae to soil bacteria, and its response to changing nutrient availability, we planted young beech trees (Fagus sylvatica) into 'split-root' boxes, dividing their root systems into two disconnected soil compartments. Each of these compartments was separated from a litter compartment by a mesh penetrable for fungal hyphae, but not for roots. Plants were exposed to a 13C-CO2-labeled atmosphere, while 15N-labeled ammonium and amino acids were added to one side of the split-root system. We found a rapid transfer of recent photosynthates via ectomycorrhizal hyphae to bacteria in root-distant soil areas. Fungal and bacterial phospholipid fatty acid (PLFA) biomarkers were significantly enriched in hyphae-exclusive compartments 24 h after 13C-CO2-labeling. Isotope imaging with nanometer-scale secondary ion mass spectrometry (NanoSIMS) allowed for the first time in situ visualization of plant-derived C and N taken up by an extraradical fungal hypha, and in microbial cells thriving on hyphal surfaces. When N was added to the litter compartments, bacterial biomass, and the amount of incorporated 13C strongly declined. Interestingly, this effect was also observed in adjacent soil compartments where added N was only available for bacteria through hyphal transport, indicating that ectomycorrhizal fungi were acting on soil bacteria. Together, our results demonstrate that (i) ectomycorrhizal hyphae rapidly transfer plant-derived C to bacterial communities in root-distant areas, and (ii) this transfer promptly responds to changing soil nutrient conditions.", "keywords": ["Hyphosphere priming", "DYNAMICS", "0301 basic medicine", "PLFAs", "Microbiology", "ectomycorrhiza", "03 medical and health sciences", "Mycorrhizosphere", "MICROBIAL COMMUNITY COMPOSITION", "NanoSIMS", "hyphal carbon transfer", "hyphosphere bacteria", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "IDENTIFICATION", "RHIZOSPHERE", "15. Life on land", "QR1-502", "EXTRACTION METHOD", "Ectomycorrhiza", "ORGANIC-MATTER", "MYCORRHIZAL FUNGI", "hyphosphere priming", "mycorrhizosphere", "Hyphal carbon transfer", "106022 Microbiology", "FATTY-ACIDS", "Hyphosphere bacteria", "BAYESIAN CLASSIFIER", "CARBON ALLOCATION"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2019.00168"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2019.00168", "name": "item", "description": "10.3389/fmicb.2019.00168", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2019.00168"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-26T00:00:00Z"}}, {"id": "11353/10.1146400", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:05:29Z", "type": "Journal Article", "created": "2019-02-26", "title": "Rapid Transfer of Plant Photosynthates to Soil Bacteria via Ectomycorrhizal Hyphae and Its Interaction With Nitrogen Availability", "description": "Plant roots release recent photosynthates into the rhizosphere, accelerating decomposition of organic matter by saprotrophic soil microbes ('rhizosphere priming effect') which consequently increases nutrient availability for plants. However, about 90% of all higher plant species are mycorrhizal, transferring a significant fraction of their photosynthates directly to their fungal partners. Whether mycorrhizal fungi pass on plant-derived carbon (C) to bacteria in root-distant soil areas, i.e., incite a 'hyphosphere priming effect,' is not known. Experimental evidence for C transfer from mycorrhizal hyphae to soil bacteria is limited, especially for ectomycorrhizal systems. As ectomycorrhizal fungi possess enzymatic capabilities to degrade organic matter themselves, it remains unclear whether they cooperate with soil bacteria by providing photosynthates, or compete for available nutrients. To investigate a possible C transfer from ectomycorrhizal hyphae to soil bacteria, and its response to changing nutrient availability, we planted young beech trees (Fagus sylvatica) into 'split-root' boxes, dividing their root systems into two disconnected soil compartments. Each of these compartments was separated from a litter compartment by a mesh penetrable for fungal hyphae, but not for roots. Plants were exposed to a 13C-CO2-labeled atmosphere, while 15N-labeled ammonium and amino acids were added to one side of the split-root system. We found a rapid transfer of recent photosynthates via ectomycorrhizal hyphae to bacteria in root-distant soil areas. Fungal and bacterial phospholipid fatty acid (PLFA) biomarkers were significantly enriched in hyphae-exclusive compartments 24 h after 13C-CO2-labeling. Isotope imaging with nanometer-scale secondary ion mass spectrometry (NanoSIMS) allowed for the first time in situ visualization of plant-derived C and N taken up by an extraradical fungal hypha, and in microbial cells thriving on hyphal surfaces. When N was added to the litter compartments, bacterial biomass, and the amount of incorporated 13C strongly declined. Interestingly, this effect was also observed in adjacent soil compartments where added N was only available for bacteria through hyphal transport, indicating that ectomycorrhizal fungi were acting on soil bacteria. Together, our results demonstrate that (i) ectomycorrhizal hyphae rapidly transfer plant-derived C to bacterial communities in root-distant areas, and (ii) this transfer promptly responds to changing soil nutrient conditions.", "keywords": ["Hyphosphere priming", "DYNAMICS", "0301 basic medicine", "PLFAs", "Microbiology", "ectomycorrhiza", "03 medical and health sciences", "Mycorrhizosphere", "MICROBIAL COMMUNITY COMPOSITION", "NanoSIMS", "hyphal carbon transfer", "hyphosphere bacteria", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "IDENTIFICATION", "RHIZOSPHERE", "15. Life on land", "QR1-502", "EXTRACTION METHOD", "Ectomycorrhiza", "ORGANIC-MATTER", "MYCORRHIZAL FUNGI", "hyphosphere priming", "mycorrhizosphere", "Hyphal carbon transfer", "106022 Microbiology", "FATTY-ACIDS", "Hyphosphere bacteria", "BAYESIAN CLASSIFIER", "CARBON ALLOCATION"]}, "links": [{"href": "https://doi.org/11353/10.1146400"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11353/10.1146400", "name": "item", "description": "11353/10.1146400", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11353/10.1146400"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-26T00:00:00Z"}}, {"id": "21.11116/0000-000D-41C9-7", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-31T07:06:05Z", "type": "Journal Article", "created": "2023-06-08", "title": "Drought intensity alters productivity, carbon allocation and plant nitrogen uptake in fast versus slow grassland communities", "description": "Abstract

Grasslands face more frequent and extreme droughts; yet, their responses to increasing drought intensity are poorly understood. Increasing drought intensity likely triggers abrupt shifts (thresholds) in grassland ecosystem functioning which can implicate recovery trajectories.

Here, we determined how drought intensity affects plant productivity, and plant\uffe2\uff80\uff93soil carbon (C) and nitrogen (N) cycling. We exposed model grassland plant communities with contrasting resource acquisition strategies (a fast\uffe2\uff80\uff90 vs a slow\uffe2\uff80\uff90strategy plant community), to a gradient of drought intensity. The drought gradient ranged from well\uffe2\uff80\uff90watered to severely water\uffe2\uff80\uff90limited conditions. We identified thresholds of plant community productivity (above\uffe2\uff80\uff90ground biomass) at peak drought and 2\uffe2\uff80\uff89months after re\uffe2\uff80\uff90wetting, and measured net ecosystem exchange and ecosystem respiration of C\uffc2\uffa0throughout the drought and recovery phases. At peak drought and 1\uffe2\uff80\uff89week after re\uffe2\uff80\uff90wetting, we traced recently acquired C from plants to the soil and into microbial biomass and fatty acids using 13C pulse labelling, and measured plant and soil N.

At peak drought, slow\uffe2\uff80\uff90strategy plant communities were more drought resistant than fast\uffe2\uff80\uff90strategy communities, as the threshold in plant productivity occurred at a higher drought intensity for the slow\uffe2\uff80\uff90 than the fast\uffe2\uff80\uff90strategy community. Shortly after re\uffe2\uff80\uff90wetting, microbial uptake of recent plant\uffe2\uff80\uff90assimilated C increased with increasing past drought intensity, coinciding with an increase in soil N availability and leaf N. Threshold responses to drought intensity at peak drought translated into non\uffe2\uff80\uff90linear recovery responses, with greater compensatory growth in the fast\uffe2\uff80\uff90strategy community. At peak drought, increasing drought intensity reduced C uptake and increased relative C partitioning to leaves and microbial biomass. Upon re\uffe2\uff80\uff90wetting, plant community strategy mediated drought intensity effects on plant and soil C and N dynamics and plant recovery trajectories. The fast\uffe2\uff80\uff90strategy community recovered quickly, with higher leaf N than the slow community, while the slow community increased C allocation to microbial biomass.

Synthesis. Our findings highlight that C and N dynamics in the plant\uffe2\uff80\uff93soil system display non\uffe2\uff80\uff90linear responses to increasing drought intensity both during and after drought, which has implications for plant community recovery trajectories.

Carbon (C) assimilation can be severely impaired during periods of environmental stress, like drought or defoliation, making trees heavily dependent on the use of C reserve pools for survival; yet, the dynamics of reserve use during periods of reduced C supply are still poorly understood. We used stem girdling in mature poplar trees (Populus tremula L. hybrids), a lipid-storing species, to permanently interrupt the phloem C transport and induced C shortage in the isolated stem section below the girdle and monitored metabolic activity during three campaigns in the growing seasons of 2018, 2019 and 2021. We measured respiratory fluxes (CO2 and O2), non-structural carbon concentration, the respiratory substrate (based on isotopic analysis and CO2/O2 ratio) and the age of the respiratory substrate (based on radiocarbon analysis). Our study shows that poplar trees can survive long periods of reduced C supply from the canopy by switching in metabolism from recent carbohydrates to older storage pools with a potential mixture of respiratory substrates, including lipids. This mechanism of stress resilience can explain why tree decline may take many years before death occurs.