In earlier work, we compared the amount of newly fixed nitrogen (N, as synthetic fertilizer and biologically fixed N) entering agricultural systems globally to the total emission of nitrous oxide (N 2 O). We obtained an N 2 O emission factor (EF) of 3\uffe2\uff80\uff935%, and applied it to biofuel production. For \uffe2\uff80\uff98first-generation\uffe2\uff80\uff99 biofuels, e.g. biodiesel from rapeseed and bioethanol from corn (maize), that require N fertilizer, N 2 O from biofuel production could cause (depending on N uptake efficiency) as much or more global warming as that avoided by replacement of fossil fuel by the biofuel. Our subsequent calculations in a follow-up paper, using published life cycle analysis (LCA) models, led to broadly similar conclusions. The N 2 O EF applies to agricultural crops in general, not just to biofuel crops, and has made possible a top-down estimate of global emissions from agriculture. Independent modelling by another group using bottom-up IPCC inventory methodology has shown good agreement at the global scale with our top-down estimate. Work by Davidson showed that the rate of accumulation of N 2 O in the atmosphere in the late nineteenth and twentieth centuries was greater than that predicted from agricultural inputs limited to fertilizer N and biologically fixed N (Davidson, E. A. 2009 Nat. Geosci . 2 , 659\uffe2\uff80\uff93662.). However, by also including soil organic N mineralized following land-use change and NO x deposited from the atmosphere in our estimates of the reactive N entering the agricultural cycle, we have now obtained a good fit between the observed atmospheric N 2 O concentrations from 1860 to 2000 and those calculated on the basis of a 4 per cent EF for the reactive N.
", "keywords": ["2. Zero hunger", "Air Pollutants", "330", "Climate", "Nitrous Oxide", "Agriculture", "15. Life on land", "Nitrification", "01 natural sciences", "7. Clean energy", "630", "Soil", "13. Climate action", "Biofuels", "Denitrification", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1098/rstb.2011.0313"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Philosophical%20Transactions%20of%20the%20Royal%20Society%20B%3A%20Biological%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1098/rstb.2011.0313", "name": "item", "description": "10.1098/rstb.2011.0313", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1098/rstb.2011.0313"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-05-05T00:00:00Z"}}, {"id": "10.1007/s11104-011-0759-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:14:48Z", "type": "Journal Article", "created": "2011-03-08", "title": "Effect Of Biochar Amendment On The Soil-Atmosphere Exchange Of Greenhouse Gases From An Intensive Subtropical Pasture In Northern New South Wales, Australia", "description": "We assessed the effect of biochar incorporation into the soil on the soil-atmosphere exchange of the greenhouse gases (GHG) from an intensive subtropical pasture. For this, we measured N2O, CH4 and CO2 emissions with high temporal resolution from April to June 2009 in an existing factorial experiment where cattle feedlot biochar had been applied at 10\u00a0t\u00a0ha\u22121 in November 2006. Over the whole measurement period, significant emissions of N2O and CO2 were observed, whereas a net uptake of CH4 was measured. N2O emissions were found to be highly episodic with one major emission pulse (up to 502\u00a0\u03bcg N2O-N m\u22122 h\u22121) following heavy rainfall. There was no significant difference in the net flux of GHGs from the biochar amended vs. the control plots. Our results demonstrate that intensively managed subtropical pastures on ferrosols in northern New South Wales of Australia can be a significant source of GHG. Our hypothesis that the application of biochar would lead to a reduction in emissions of GHG from soils was not supported in this field assessment. Additional studies with longer observation periods are needed to clarify the long term effect of biochar amendment on soil microbial processes and the emission of GHGs under field conditions.", "keywords": ["2. Zero hunger", "Biochar", "Nitrogen", "13. Climate action", "Denitrification", "0401 agriculture", " forestry", " and fisheries", "Improved pasture", "04 agricultural and veterinary sciences", "15. Life on land", "Nitrification", "Carbon"]}, "links": [{"href": "https://eprints.qut.edu.au/42094/1/42094A.pdf"}, {"href": "https://doi.org/10.1007/s11104-011-0759-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11104-011-0759-1", "name": "item", "description": "10.1007/s11104-011-0759-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11104-011-0759-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-03-08T00:00:00Z"}}, {"id": "10.1007/s00442-012-2578-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:14:23Z", "type": "Journal Article", "created": "2013-01-07", "title": "Effects Of Drought And N-Fertilization On N Cycling In Two Grassland Soils", "description": "Open AccessOecologia, 171 (3)", "keywords": ["[SDE] Environmental Sciences", "N2O fluxes", "550", "functional genes", "Nitrogen", "[SDV]Life Sciences [q-bio]", "Climate", "Climate Change", "Nitrification and denitrification", "enzyme activites", "Urine", "630", "10127 Institute of Evolutionary Biology and Environmental Studies", "Soil", "Quantitative PCR", "Climate change; Enzyme activities; Functional genes; Quantitative PCR; Nitrification and denitrification; N2O fluxes", "[SDV.BV]Life Sciences [q-bio]/Vegetal Biology", "Animals", "Climate change", "Enzyme activities", "[SDV.BV] Life Sciences [q-bio]/Vegetal Biology", "Ecosystem", "Soil Microbiology", "Functional genes", "Nitrogen Cycle", "Plants", "Archaea", "Droughts", "[SDV] Life Sciences [q-bio]", "1105 Ecology", " Evolution", " Behavior and Systematics", "climate change", "Genes", " Bacterial", "[SDE]Environmental Sciences", "quantitative PCR", "Denitrification", "570 Life sciences; biology", "590 Animals (Zoology)", "Cattle", "nitrification and denitrification"]}, "links": [{"href": "https://doi.org/10.1007/s00442-012-2578-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-012-2578-3", "name": "item", "description": "10.1007/s00442-012-2578-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-012-2578-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-01-08T00:00:00Z"}}, {"id": "10451/51022", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:24:39Z", "type": "Journal Article", "created": "2017-10-10", "title": "Nitric Oxide Accumulation: The Evolutionary Trigger for Phytopathogenesis", "description": "Many publications highlight the importance of nitric oxide (NO) in plant-bacteria interactions, either in the promotion of health and plant growth or in pathogenesis. However, the role of NO in the signaling between bacteria and plants and in the fate of their interaction, as well as the reconstruction of their interactive evolution, remains largely unknown. Despite the complexity of the evolution of life on Earth, we explore the hypothesis that denitrification and aerobic respiration were responsible for local NO accumulation, which triggered primordial antagonistic biotic interactions, namely the first phytopathogenic interactions. N-oxides, including NO, could globally accumulate via lightning synthesis in the early anoxic ocean and constitute pools for the evolution of denitrification, considered an early step of the biological nitrogen cycle. Interestingly, a common evolution may be proposed for components of denitrification and aerobic respiration pathways, namely for NO and oxygen reductases, a theory compatible with the presence of low amounts of oxygen before the great oxygenation event (GOE), which was generated by Cyanobacteria. During GOE, the increase in oxygen caused the decrease of Earth's temperature and the consequent increase of oxygen dissolution and availability, making aerobic respiration an increasingly dominant trait of the expanding mesophilic lifestyle. Horizontal gene transfer was certainly important in the joint expansion of mesophily and aerobic respiration. First denitrification steps lead to NO formation through nitrite reductase activity, and NO may further accumulate when oxygen binds NO reductase, resulting in denitrification blockage. The consequent transient NO surplus in an oxic niche could have been a key factor for a successful outcome of an early denitrifying prokaryote able to scavenge oxygen by NO/oxygen reductase or by an independent heterotrophic aerobic respiration pathway. In fact, NO surplus could result in toxicity causing 'the first disease' in oxygen-producing Cyanobacteria. We inspected in bacteria the presence of sequences similar to the NO-producing nitrite reductase nirS gene of Thermus thermophilus, an extreme thermophilic aerobe of the Thermus/Deinococcus group, which constitutes an ancient lineage related to Cyanobacteria. In silico analysis revealed the relationship between the presence of nirS genes and phytopathogenicity in Gram-negative bacteria.", "keywords": ["aerobic respiration", "0301 basic medicine", "denitrification", "Thermus thermophilus", "nitrite reductase NirS", "Horizontal gene transfer", "Denitrific", "Microbiology", "QR1-502", "Nitrite reductase NirS", "Ationerobic respiration", "03 medical and health sciences", "13. Climate action", "horizontal gene transfer"]}, "links": [{"href": "https://repositorio.ulisboa.pt/bitstream/10451/51022/1/Santana%20et%20al%202017.pdf"}, {"href": "https://doi.org/10451/51022"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10451/51022", "name": "item", "description": "10451/51022", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10451/51022"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-10-10T00:00:00Z"}}, {"id": "10.1016/j.agee.2022.108182", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:15:19Z", "type": "Journal Article", "created": "2022-09-21", "title": "Liming modifies greenhouse gas fluxes from soils: A meta-analysis of biological drivers", "description": "Acidic soils cover about 30% of the world's land. Liming is a management practice applied worldwide to reduce the negative effects of acidification on soil fertility and plant growth. Liming also affects the biotic and abiotic soil properties controlling the production and consumption of the greenhouse gases (GHGs) carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4). Although our understanding of how liming regulates net GHG emissions is increasing, the impact of liming on soil biological drivers of GHG emissions has not been quantitatively synthesized. Here we conducted a global meta-analysis using 1474 paired observations from 124 studies to explore the responses of GHG emissions to liming, with a focus on soil biological factors. We show that the N2O mitigation capacity of liming could be linked to (i) increases in bacterial abundance of N2O reductase genes (NosZ) and decreases in fungi:bacteria ratio, both contributing to a lower N2O:N2 product ratio of denitrification; and (ii) reductions in soil mineral nitrogen (N) via stimulation of plant N uptake. The limited evidence available indicates that liming reduced CH4 emissions and the abundance of methanogens, but it had no effect on CH4 uptake and abundance of methanotrophs. Liming-induced increases in soil CO2 emissions can be explained by higher heterotrophic and/or autotrophic respiration. The strong coupling between liming effects on GHG emissions and on soil microbial communities involved in GHG production and consumption can be used to identify strategies to reduce GHGs in response to liming, and to improve process-based models for better predictions of soil GHG emissions.
", "keywords": ["2. Zero hunger", "Biological drivers", "04 agricultural and veterinary sciences", "15. Life on land", "Nitrification", "01 natural sciences", "6. Clean water", "13. Climate action", "Greenhouse gas emissions", "11. Sustainability", "Denitrification", "0401 agriculture", " forestry", " and fisheries", "Liming", "Soil acidification", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.agee.2022.108182"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2022.108182", "name": "item", "description": "10.1016/j.agee.2022.108182", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2022.108182"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-01T00:00:00Z"}}, {"id": "10.1002/jsfa.4533", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:13:58Z", "type": "Journal Article", "created": "2011-07-27", "title": "Influence Of Fertilisation Regimes On A Nosz-Containing Denitrifying Community In A Rice Paddy Soil", "description": "AbstractBACKGROUND: Denitrification is a microbial process that has received considerable attention during the past decade since it can result in losses of added nitrogen fertilisers from agricultural soils. Paddy soil has been known to have strong denitrifying activity, but the denitrifying microorganisms responsible for fertilisers in paddy soil are not well known. The objective of this study was to explore the impacts of 17\uffe2\uff80\uff90year application of inorganic and organic fertiliser (rice straw) on the abundance and composition of a nosZ\uffe2\uff80\uff90denitrifier community in paddy soil. Soil samples were collected from CK plots (no fertiliser), N (nitrogen fertiliser), NPK (nitrogen, phosphorus and potassium fertilisers) and NPK + OM (NPK plus organic matter). The nitrous oxide reductase gene (nosZ) community composition was analysed using terminal restriction fragment length polymorphism, and the abundance was determined by quantitative PCR.
RESULTS: Both the largest abundance of nosZ\uffe2\uff80\uff90denitrifier and the highest potential denitrifying activity (PDA) occurred in the NPK + OM treatment with about four times higher than that in the CK and two times higher than that in the N and NPK treatments (no significant difference). Denitrifying community composition differed significantly among fertilisation treatments except for the comparison between CK and N treatments. Of the measured abiotic factors, total organic carbon was significantly correlated with the observed differences in community composition and abundance (P < 0.01 by Monte Carlo permutation).
CONCLUSION: This study shows that the addition of different fertilisers affects the size and composition of the nosZ\uffe2\uff80\uff90denitrifier community in paddy soil. Copyright \uffc2\uffa9 2011 Society of Chemical Industry
", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Bacteria", "Nitrogen", "0402 animal and dairy science", "Agriculture", "Oryza", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "Carbon", "Soil", "03 medical and health sciences", "Genes", " Bacterial", "Denitrification", "0405 other agricultural sciences", "Fertilizers", "Oxidoreductases", "Monte Carlo Method", "Polymorphism", " Restriction Fragment Length", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1002/jsfa.4533"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.4533", "name": "item", "description": "10.1002/jsfa.4533", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.4533"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-07-27T00:00:00Z"}}, {"id": "10.1007/s00374-012-0752-8", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:14:19Z", "type": "Journal Article", "created": "2012-11-22", "title": "Nitrogen Dynamics Of Anaerobically Digested Slurry Used To Fertilize Paddy Fields", "description": "To determine nitrogen (N) fate and environmental impact of applying anaerobic digestion slurry (ADS) to rice paddy (Oryza sativa L.), a field experiment was established using three treatments based on contrasting N application rate. The ADS (with ammonium-N accounting for >80 % of total N) treatment at a conventional application rate of 270 kg N ha\u22121 was compared to a negative control (no N fertilizer) and a positive control of urea applied at 270 kg N ha\u22121. The N budget showed the following distribution of applied N from ADS and urea: 41.3 \u00b1 5.1 % for ADS and 36.6 \u00b1 4.4 % for urea recovered by the rice plant (including straw, grain, and root), 16.4 \u00b1 3.7 % for ADS and 7.4 \u00b1 1.8 % for urea lost via ammonia volatilization, 0.26 \u00b1 0.15 % for ADS and 0.15 \u00b1 0.12 % for urea lost by direct N2O emission, 1.9 \u00b1 0.5 % for ADS and 2.3 \u00b1 0.8 % for urea leached downward, 0.70 \u00b1 0.15 % for ADS and 0.67 \u00b1 0.12 % for urea discharged with floodwater drainage, and 39.4 \u00b1 8.4 % for ADS and 53.0 \u00b1 9.1 % for urea retained by soil or lost by N2 emission. Compared to urea application, ADS application impacts the environment mainly through gaseous N losses rather than water N losses. ADS application had a positive impact on rice grain yield and reduced chemical fertilizer use. Considering the wide distribution of paddy fields and the ever-increasing quantities of ADS, ADS may serve as a valuable N source for rice cultivation, although mitigating ammonia and N2O losses should be further investigated.", "keywords": ["2. Zero hunger", "Agricultural and Veterinary Sciences", "Agronomy & Agriculture", "04 agricultural and veterinary sciences", "Ammonia volatilization", "Biological Sciences", "7. Clean energy", "Nitrogen-use efficiency", "6. Clean water", "Anaerobically digested slurry", "Denitrification", "Paddy field", "0401 agriculture", " forestry", " and fisheries", "Zero Hunger", "Environmental Sciences"]}, "links": [{"href": "https://escholarship.org/content/qt3d16p0gn/qt3d16p0gn.pdf"}, {"href": "https://doi.org/10.1007/s00374-012-0752-8"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biology%20and%20Fertility%20of%20Soils", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00374-012-0752-8", "name": "item", "description": "10.1007/s00374-012-0752-8", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00374-012-0752-8"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-11-22T00:00:00Z"}}, {"id": "10.1007/s00442-012-2484-8", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:14:23Z", "type": "Journal Article", "created": "2012-12-27", "title": "Herbivore Trampling As An Alternative Pathway For Explaining Differences In Nitrogen Mineralization In Moist Grasslands", "description": "Studies addressing the role of large herbivores on nitrogen cycling in grasslands have suggested that the direction of effects depends on soil fertility. Via selection for high quality plant species and input of dung and urine, large herbivores have been shown to speed up nitrogen cycling in fertile grassland soils while slowing down nitrogen cycling in unfertile soils. However, recent studies show that large herbivores can reduce nitrogen mineralization in some temperate fertile soils, but not in others. To explain this, we hypothesize that large herbivores can reduce nitrogen mineralization in loamy or clay soils through soil compaction, but not in sandy soils. Especially under wet conditions, strong compaction in clay soils can lead to periods of soil anoxia, which reduces decomposition of soil organic matter and, hence, N mineralization. In this study, we use a long-term (37-year) field experiment on a salt marsh to investigate the hypothesis that the effect of large herbivores on nitrogen mineralization depends on soil texture. Our results confirm that the presence of large herbivores decreased nitrogen mineralization rate in a clay soil, but not in a sandy soil. By comparing a hand-mown treatment with a herbivore-grazed treatment, we show that these differences can be attributed to herbivore-induced changes in soil physical properties rather than to above-ground biomass removal. On clay soil, we find that large herbivores increase the soil water-filled porosity, induce more negative soil redox potentials, reduce soil macrofauna abundance, and reduce decomposition activity. On sandy soil, we observe no changes in these variables in response to grazing. We conclude that effects of large herbivores on nitrogen mineralization cannot be understood without taking soil texture, soil moisture, and feedbacks through soil macrofauna into account.", "keywords": ["0106 biological sciences", "IMPACT", "Nitrogen", "01 natural sciences", "Soil fauna", "COMPACTION", "Soil", "SOIL PHYSICAL-PROPERTIES", "SALT-MARSH", "Large herbivores", "Soil texture", "Animals", "Biomass", "Herbivory", "Soil compaction", "Ecosystem", "2. Zero hunger", "UNGULATE", "national", "Water", "DENITRIFICATION", "Nitrogen Cycle", "15. Life on land", "N cycling", "YELLOWSTONE-NATIONAL-PARK", "PLANT-GROWTH", "13. Climate action", "ECOSYSTEM", "Clay", "Aluminum Silicates", "Soil moisture", "BAIT-LAMINA TEST"]}, "links": [{"href": "https://doi.org/10.1007/s00442-012-2484-8"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-012-2484-8", "name": "item", "description": "10.1007/s00442-012-2484-8", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-012-2484-8"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-12-28T00:00:00Z"}}, {"id": "10.1007/s10021-010-9341-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:14:27Z", "type": "Journal Article", "created": "2010-05-27", "title": "Size Of Precipitation Pulses Controls Nitrogen Transformation And Losses In An Arid Patagonian Ecosystem", "description": "Arid ecosystems receive precipitation pulses of different sizes that may differentially affect nitrogen (N) losses and N turnover during the growing season. We designed a rainfall manipulation experiment in the Patagonian steppe, southern Argentina, where we simulated different precipitation patterns by adding the same amount of water in evenly spaced three-small rainfall events or in one-single large rainfall event, three times during a growing season. We measured the effect of the size of rainfall pulses on N mineralization and N losses by denitrification, ammonia volatilization, and nitrate and ammonia leaching. Irrigation pulses stimulated N mineralization (P < 0.05), with small and frequent pulses showing higher responses than large pulses (P < 0.10). Irrigation effects were transient and did not result in changes in seasonal net N mineralization suggesting a long-term substrate limitation. Water pulses stimulated gaseous N losses by denitrification, with large pulses showing higher responses than small pulses (P < 0.05), but did not stimulate ammonia volatilization. Nitrate leaching also was higher after large than after small precipitation events (P < 0.05). Small events produced higher N transformations and lower N losses by denitrification and nitrate leaching than large events, which would produce higher N availability for plant growth. Climate change is expected to increase the frequency of extreme precipitation events and the proportion of large to small rainfall events. Our results suggest that these changes would result in reduced N availability and a competitive advantage for deep-rooted species that prefer nitrate over ammonia. Similarly, the ammonium:nitrate ratio might decrease because large events foster nitrate losses but not ammonium losses.", "keywords": ["2. Zero hunger", "Ammonia Volatilization", "Precipitation Pulses", "Arid Ecosystems", "Patagonian Steppe", "Nitrate Leaching", "Soil Inorganic N", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "Net N Mineralization", "13. Climate action", "https://purl.org/becyt/ford/1.6", "Denitrification", "0401 agriculture", " forestry", " and fisheries", "https://purl.org/becyt/ford/1", "Nitrogen-Water Interactions", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s10021-010-9341-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecosystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10021-010-9341-6", "name": "item", "description": "10.1007/s10021-010-9341-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10021-010-9341-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-05-28T00:00:00Z"}}, {"id": "10.1007/s10705-007-9091-9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:14:39Z", "type": "Journal Article", "created": "2007-02-23", "title": "Effects Of Fertiliser Type And The Presence Or Absence Of Plants On Nitrous Oxide Emissions From Irrigated Soils", "description": "Open AccessPeer reviewed", "keywords": ["2. Zero hunger", "N20 emission", "Municipal solid waste", "Composts", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "12. Responsible consumption", "Denitrification losses", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Pig slurry", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s10705-007-9091-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nutrient%20Cycling%20in%20Agroecosystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10705-007-9091-9", "name": "item", "description": "10.1007/s10705-007-9091-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10705-007-9091-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-02-24T00:00:00Z"}}, {"id": "10.1016/j.agee.2014.02.021", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:15:15Z", "type": "Journal Article", "created": "2014-03-15", "title": "Biochar Does Not Affect Soil N-Transformations Or Microbial Community Structure Under Ruminant Urine Patches But Does Alter Relative Proportions Of Nitrogen Cycling Bacteria", "description": "Abstract Nitrogen (N) cycling, especially denitrification, can be significantly altered when biochar is used as a soil conditioner. These alterations in N-cycling have been attributed to a combination of physicochemical change, alterations in microbial community ecology and pervading climatic conditions. This study investigated seasonal bacterial community change over two years in combination with a short-term winter study of N-transformations under bovine urine patches. A silt-loam pastoral soil in Canterbury, New Zealand was amended with either 0, 15 or 30\u00a0t\u00a0ha \u22121 of Pinus radiata biochar (pyrolysed at \u223c450\u00a0\u00b0C) and bovine urine was added to patches within the 0 and 30\u00a0t\u00a0ha \u22121 biochar amended plots (designated as 0\u00a0U and 30\u00a0U treatments, where U indicates \u2018urine\u2019). No discernible differences in bacterial community structure were observed during the two year study or the short term N-transformation study when comparing non-amended and biochar-amended soil. Differences in bacterial community structure were only evident when comparing seasons, with data pertaining to each season from successive years clustering together. During the short-term N-transformation study, bacterial communities formed 3 distinct clusters corresponding to elevated levels of urine derived NH 4 + -N (days 0\u201310), increases in NO 3 \u2212 -N and N 2 O (days 10\u201322) and a decline in NO 3 \u2212 -N and N 2 O (day 20 onward). Biochar amendment did increase the relative abundance of up to 50% of individual operational taxonomic units (OTUs or \u2018species\u2019), including key nitrite oxidisers and nitrate reducers. Biochar amendment did not affect the concentrations of inorganic-N compounds. The nir S (nitrite reductase) gene became elevated in the 30\u00a0U treatment relative to the 0\u00a0U treatment \u223c10 days after the initial urine application. The nos Z (nitrous oxide reductase) gene became elevated in the 30\u00a0U plots during the latter part of the experiment. Conclusions: \u2022 Biochar did not have a significant impact on the microbial community structure in pastoral soil over the course of two years. \u2022 The relative proportion of nitrifiers and denitrifiers increased in biochar amended soils subjected to large influxes of urine derived N. \u2022 Differences in N-transformation dynamics in the presence of biochar during the winter months were not statistically significant.", "keywords": ["2. Zero hunger", "N\u2082O emissions", "570", "denitrification", "bovine urine", "silt-loam soil", "ANZSRC::30 Agricultural", "04 agricultural and veterinary sciences", "15. Life on land", "soil microbial ecology", "winter", "nitrification", "630", "6. Clean water", "veterinary and food sciences", "T-RFLP", "new generation sequencing", "13. Climate action", "ANZSRC::41 Environmental sciences", "XXXXXX - Unknown", "0401 agriculture", " forestry", " and fisheries", "biochar", "ANZSRC::44 Human society"], "contacts": [{"organization": "Timothy J. Clough, Kelly Hamonts, Leo M. Condron, Craig Anderson, Craig Anderson,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.agee.2014.02.021"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2014.02.021", "name": "item", "description": "10.1016/j.agee.2014.02.021", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2014.02.021"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-06-01T00:00:00Z"}}, {"id": "10.1007/s11104-012-1248-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:14:49Z", "type": "Journal Article", "created": "2012-05-04", "title": "Effects Of Simulated Drought And Nitrogen Fertilizer On Plant Productivity And Nitrous Oxide (N2o) Emissions Of Two Pastures", "description": "Open AccessISSN:0032-079X", "keywords": ["Soil acidity", "Drought", "Soil microbial C and N", "04 agricultural and veterinary sciences", "15. Life on land", "Grassland", "Nitrification", "10127 Institute of Evolutionary Biology and Environmental Studies", "Grazing", "Greenhouse gases", "Summer drought", "13. Climate action", "1110 Plant Science", "Denitrification", "570 Life sciences; biology", "590 Animals (Zoology)", "0401 agriculture", " forestry", " and fisheries", "Compensatory growth; Denitrification; Drought; Grassland; Grazing; Greenhouse gases; Soil microbial C and N; Soil acidity; Nitrification; Summer drought", "Compensatory growth", "1111 Soil Science"]}, "links": [{"href": "https://doi.org/10.1007/s11104-012-1248-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11104-012-1248-x", "name": "item", "description": "10.1007/s11104-012-1248-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11104-012-1248-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-05-05T00:00:00Z"}}, {"id": "10.1007/s11356-014-3762-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:14:57Z", "type": "Journal Article", "created": "2014-11-04", "title": "Denitrification Kinetics In Biomass- And Biochar-Amended Soils Of Different Landscape Positions", "description": "Knowledge of how biochar impacts soil denitrification kinetics as well as the mechanisms of interactions is essential in order to better predict the nitrous oxide (N2O) mitigation capacity of biochar additions. This study had multiple experiments in which the effect of three biochar materials produced from corn stover (Zea mays L.), ponderosa pine wood residue (Pinus ponderosa Douglas ex Lawson and C. Lawson), switchgrass (Panicum virgatum L.), and their corresponding biomass materials (corn stover, ponderosa pine wood residue, and switchgrass) on cumulative N2O emissions and total denitrification in soils from two different landscape positions (crest and footslope) were studied under varying water-filled pore space (40, 70, and 90% WFPS). Cumulative N2O emissions were reduced by 30 to 70% in both crest and footslope soils. The effect of biochars and biomass treatments on cumulative N2O emissions and total denitrification were only observed at \u226540% WFPS. The denitrification enzyme activity (DEA) kinetic parameters, K s (half-saturation constant), and V max (maximum DEA rate) were both significantly reduced by biochar treatments, with reductions of 70-80% in footslope soil and 80-90 % in the crest soil. The activation energy (E a) and enthalpy of activation of DEA (\u0394H) were both increased with biochar application. The trends in DEA rate constants (K s and V max) were correlated by the trends of thermodynamic parameters (activation energy E a and enthalpy of activation \u0394H) for denitrifying enzyme activity (DEA). The rate constant V max/K s evaluated the capacity of biochars to mitigate the denitrification process. Denitrifying enzyme kinetic parameters can be useful in evaluating the ability of biochars to mitigate N2O gas losses from soil.", "keywords": ["Nitrous Oxide", "Water", "04 agricultural and veterinary sciences", "15. Life on land", "Panicum", "Wood", "Zea mays", "6. Clean water", "Pinus ponderosa", "Kinetics", "Soil", "13. Climate action", "Charcoal", "Denitrification", "0401 agriculture", " forestry", " and fisheries", "Biomass"]}, "links": [{"href": "https://doi.org/10.1007/s11356-014-3762-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11356-014-3762-2", "name": "item", "description": "10.1007/s11356-014-3762-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11356-014-3762-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-11-05T00:00:00Z"}}, {"id": "10.1007/s13762-013-0250-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:15:05Z", "type": "Journal Article", "created": "2013-03-25", "title": "A poly-\u03b5-caprolactone based biofilm carrier for nitrate removal from water", "description": "Nitrate removal from water has been accomplished by heterotrophic biofilms using organic carbon as a source of reducing power. To overcome the natural limitation in organic carbon in water, a poly-e-caprolactone based biofilm carrier that serves simultaneously as a biofilm carrier and as a source of organic carbon was developed and tested in the present work. The feasibility of the new biofilm carrier for nitrate removal from water was evaluated in a packed bed reactor. The combination of size and structure provided a carrier element having high surface area and void volume, 1,170\u00a0m2/m3 and 67\u00a0%, respectively. A maximum denitrification rate of 4.4\u00a0mg\u00a0N\u2013NO3 \u2212/(L.h) (9.2\u00a0mg\u00a0N\u2013NO3 \u2212/(m2.h)) was achieved in the packed bed reactor at 20\u00a0\u00b0C and pH 7.0. Main advantages of the biofilm carrier developed in the present work are its mechanical stability in water even after biofilm formation and controlled release of organic carbon by enzymatic reactions. The proposed biotechnology to remove nitrate from groundwater is robust and easy to operate.", "keywords": ["Science & Technology", "Biofilm", "0207 environmental engineering", "02 engineering and technology", "Biofilm reactor", "01 natural sciences", "6. Clean water", "Solid carbon source", "Biofilters", "Biodegradable polymer", "Denitrification", "Packed bed reactor", "Tratamento de \u00e1guas residuais", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s13762-013-0250-z"}, {"href": "https://doi.org/10.1007/s13762-013-0250-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Environmental%20Science%20and%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s13762-013-0250-z", "name": "item", "description": "10.1007/s13762-013-0250-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s13762-013-0250-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-03-26T00:00:00Z"}}, {"id": "10.1016/j.agee.2013.05.001", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:15:15Z", "type": "Journal Article", "created": "2013-05-29", "title": "Earthworms Can Increase Nitrous Oxide Emissions From Managed Grassland: A Field Study", "description": "Earthworms are important in determining the greenhouse gas (GHG) balance of soils. In laboratory studies they have been shown to increase emissions of the potent GHG nitrous oxide (N2O). Here we test whether these earthworm-induced N2O emissions also occur in the field. We quantified N2O emissions in managed grassland in two different seasons (spring and autumn), applying two different types of fertilizer (organic and artificial fertilizer) and under two earthworm densities (175 individuals and 350 individuals m(-2)) of the species Lumbricus rubellus (Hoffmeister). We found an increase in earthworm-induced N2O emissions of 286 and 394% in autumn for low and high earthworm densities (P = 0.044 and P = 0.007, respectively). There were no effects of earthworms on N2O emissions in spring. Fertilizer additions significantly increased cumulative N2O emissions and grass N content in spring and autumn. For grass N content interactions between earthworm addition and fertilizer type existed in both seasons. Our results suggest that the pathways through which earthworms affect N cycling (and thereby N2O emission) differ with weather conditions. We postulate that in spring the dry weather conditions overruled any earthworm effects, whereas in autumn earthworms mainly improved soil aeration and thereby increased both plant N uptake and diffusion of N2O to the atmosphere. While we showed the presence of earthworm-induced N2O emissions in managed grassland under field conditions for the first time, the nature and intensity of the earthworm effect in the field is conditional on soil physicochemical parameters and thereby on meteorological and seasonal dynamics. (C) 2013 Elsevier B.V. All rights reserved.", "keywords": ["2. Zero hunger", "agroecosystem", "n2o emission", "04 agricultural and veterinary sciences", "15. Life on land", "carbon-dioxide", "fluxes", "soil", "crop residue", "13. Climate action", "peat", "gut", "0401 agriculture", " forestry", " and fisheries", "mesocosms", "nitrifier denitrification"]}, "links": [{"href": "https://doi.org/10.1016/j.agee.2013.05.001"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2013.05.001", "name": "item", "description": "10.1016/j.agee.2013.05.001", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2013.05.001"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-07-01T00:00:00Z"}}, {"id": "10.1016/j.ese.2020.100013", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:15:58Z", "type": "Journal Article", "created": "2020-01-13", "title": "Microbial electrochemistry for bioremediation", "description": "Lack of suitable electron donors or acceptors is in many cases the key reason for pollutants to persist in the environment. Externally supplementation of electron donors or acceptors is often difficult to control and/or involves chemical additions with limited lifespan, residue formation or other adverse side effects. Microbial electrochemistry has evolved very fast in the past years - this field relates to the study of electrochemical interactions between microorganisms and solid-state electron donors or acceptors. Current can be supplied in such so-called bioelectrochemical systems (BESs) at low voltage to provide or extract electrons in a very precise manner. A plethora of metabolisms can be linked to electrical current now, from metals reductions to denitrification and dechlorination. In this perspective, we provide an overview of the emerging applications of BES and derived technologies towards the bioremediation field and outline how this approach can be game changing.", "keywords": ["0301 basic medicine", "AUTOTROPHIC DENITRIFICATION", "elecetrobioremediation", "Bioremediaci\u00f3", "FUEL-CELLS", "Environmental technology. Sanitary engineering", "Microbial biotechnology", "01 natural sciences", "POLYCYCLIC AROMATIC-HYDROCARBONS", "03 medical and health sciences", "WASTE-WATER", "DECHLORINATION", "TD Environmental technology. Sanitary engineering", "Electrochemistry", "POLLUTANTS", "GE1-350", "TD1-1066", "0105 earth and related environmental sciences", "NITRATE-CONTAMINATED GROUNDWATER", "ENVIRONMENTAL REMEDIATION", "Q Science (General)", "QR Microbiology", "NITROGEN REMOVAL", "6. Clean water", "Environmental sciences", "Electroqu\u00edmica", "ORGANIC", "BIOELECTROCHEMICAL SYSTEMS", "13. Climate action", "Earth and Environmental Sciences", "Perspective", "Biotecnologia microbiana", "Bioremediation"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/540323/1/1-s2.0-S2666498420300053-main.pdf"}, {"href": "https://doi.org/10.1016/j.ese.2020.100013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Ecotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ese.2020.100013", "name": "item", "description": "10.1016/j.ese.2020.100013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ese.2020.100013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-01T00:00:00Z"}}, {"id": "10.1016/j.apsoil.2010.04.006", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:15:28Z", "type": "Journal Article", "created": "2010-05-22", "title": "Effect Of The Endogeic Earthworm Pontoscolex Corethrurus On The Microbial Structure And Activity Related To Co2 And N2o Fluxes From A Tropical Soil (Madagascar)", "description": "Abstract The objective of this laboratory study was to determine the influence of a tropical endogeic earthworm, Pontoscolex corethrurus , on CO 2 and N 2 O fluxes from a tropical Ferralsol and microorganisms potentially involved in these gases emissions. CO 2 and N 2 O fluxes were measured during 35 days from soil mesocosms with and without earthworms. At the end of the incubation, 7% of soil was egested as cast in the earthworm treatment. Then, casts which may be aged from few hours to 35 days old were isolated from non-ingested soil. Different descriptive parameters (activity, density, and structure) of the microbial communities were investigated in the control, the non-ingested soils, and the casts. Quantitative PCR of denitrification genes encoding the nitrite ( nirK ) and nitrous oxide ( nosZ ) reductases was used to study denitrifier density in the earthworm casts. The presence of P. corethrurus induced a significant increase in CO 2 emissions but did not affect N 2 O fluxes when measured at mesocosm level. Despite the absence of significant differences in C and N contents between soils and casts, the near infra-red spectra analysis clearly underlined a specific organic signature for the casts. Fungal and bacterial biomass significantly decreased (\u223c2-fold) in casts compared to parent soil, but the fungal-to-bacterial ratio was not modified by the earthworm casting activity. Data suggested that bacterial communities, especially denitrifiers, were modified in casts. The relative abundance of nirK and nosZ genes increased in the casts while the genetic structures of total bacteria and denitrifying communities were slightly modified in the casts. This study highlighted the importance of earthworm casts as a specific soil habitat where a subset of soil functional bacterial communities (such as denitrifiers) found favourable condition for their growth. However the effect of P. corethrurus was less evident when up-scaling from casts to mesocosm level.", "keywords": ["Pontoscolex corethrurus", "Quantitative PCR", "Denitrification genes", "Madagascar", "0401 agriculture", " forestry", " and fisheries", "Microbial activity potentials", "04 agricultural and veterinary sciences", "01 natural sciences", "630", "FAME", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.apsoil.2010.04.006"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Soil%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.apsoil.2010.04.006", "name": "item", "description": "10.1016/j.apsoil.2010.04.006", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.apsoil.2010.04.006"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-07-01T00:00:00Z"}}, {"id": "10.1016/j.biortech.2018.09.044", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:15:35Z", "type": "Journal Article", "created": "2018-09-10", "title": "Biokinetics of microbial consortia using biogenic sulfur as a novel electron donor for sustainable denitrification", "description": "In this study, the biokinetics of autotrophic denitrification with biogenic S0 (ADBIOS) for the treatment of nitrogen pollution in wastewaters were investigated. The used biogenic S0, a by-product of gas desulfurization, was an elemental microcrystalline orthorhombic sulfur with a median size of 4.69\u202f\u00b5m and a specific surface area of 3.38\u202fm2/g, which made S0 particularly reactive and bioavailable. During denitritation, the biomass enriched on nitrite (NO2-) was capable of degrading up to 240\u202fmg/l NO2--N with a denitritation activity of 339.5\u202fmg NO2--N/g VSS\u00b7d. The use of biogenic S0 induced a low NO2--N accumulation, hindering the NO2--N negative impact on the denitrifying consortia and resulting in a specific denitrification activity of 223.0\u202fmg NO3--N/g VSS\u00b7d. Besides Thiobacillus being the most abundant genus, Moheibacter and Thermomonas were predominantly selected for denitrification and denitritation, respectively.", "keywords": ["Nitrite accumulation", "Nitrogen", "Microbial Consortia", "Biokinetics", "0211 other engineering and technologies", "Electrons", "02 engineering and technology", "Autotrophic denitrification", "Thiobacillus", "01 natural sciences", "6. Clean water", "Community structure", "12. Responsible consumption", "Kinetics", "Bioreactors", "13. Climate action", "Autotrophic denitrification; Biogenic sulfur; Nitrite accumulation; Biokinetics; Community structure", "Biogenic sulfur", "Denitrification", "Biomass", "Sulfur", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://eprints.gla.ac.uk/168662/1/168662.pdf"}, {"href": "https://www.iris.unina.it/bitstream/11588/722336/1/2018%20-%20Kostrytsia%20et%20al.%20-%20Bioresource%20Technology%20-%20Biokinetics%20of%20microbial%20consortia%20using%20biogenic%20S0.pdf"}, {"href": "https://doi.org/10.1016/j.biortech.2018.09.044"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biortech.2018.09.044", "name": "item", "description": "10.1016/j.biortech.2018.09.044", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biortech.2018.09.044"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-12-01T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2011.08.031", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:15:40Z", "type": "Journal Article", "created": "2011-09-22", "title": "Impact Of Biochar Application To A Mediterranean Wheat Crop On Soil Microbial Activity And Greenhouse Gas Fluxes", "description": "Biochar has been recently proposed as a management strategy to improve crop productivity and global warming mitigation. However, the effect of such approach on soil greenhouse gas fluxes is highly uncertain and few data from field experiments are available. In a field trial, cultivated with wheat, biochar was added to the soil (3 or 6 kg m(-2)) in two growing seasons (2008/2009 and 2009/2010) so to monitor the effect of treatments on microbial parameters 3 months and 14 months after char addition. N(2)O, CH(4) and CO(2) fluxes were measured in the field during the first year after char addition. Biochar incorporation into the soil increased soil pH (from 5.2 to 6.7) and the rates of net N mineralization, soil microbial respiration and denitrification activity in the first 3 months, but after 14 months treated and control plots did not differ significantly. No changes in total microbial biomass and net nitrification rate were observed. In char treated plots, soil N(2)O fluxes were from 26% to 79% lower than N(2)O fluxes in control plots, excluding four sampling dates after the last fertilization with urea, when N(2)O emissions were higher in char treated plots. However, due to the high spatial variability, the observed differences were rarely significant. No significant differences of CH(4) fluxes and field soil respiration were observed among different treatments, with just few exceptions. Overall the char treatments showed a minimal impact on microbial parameters and GHG fluxes over the first 14 months after biochar incorporation.", "keywords": ["Crops", " Agricultural", "Greenhouse Effect", "Nitrous Oxide", "Biochar; CH; 4; CO; 2; Denitrification; N; 2; O; Nitrification;", "630", "12. Responsible consumption", "Fertilizers", "Soil Microbiology", "Triticum", "2. Zero hunger", "CH4", "Bacteria", "N2O", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Nitrification", "6. Clean water", "Biochar", "13. Climate action", "Charcoal", "Denitrification", "0401 agriculture", " forestry", " and fisheries", "CO2", "Gases", "Methane", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2011.08.031"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2011.08.031", "name": "item", "description": "10.1016/j.chemosphere.2011.08.031", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2011.08.031"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-11-01T00:00:00Z"}}, {"id": "10.1016/j.jconhyd.2021.103797", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:26Z", "type": "Journal Article", "created": "2021-03-18", "title": "Dynamics of nitrous oxide with depth in groundwater: Insights from ambient groundwater and laboratory incubation experiments (Hesbaye chalk aquifer, Belgium)", "description": "Aquifers under agricultural areas are considered to be an indirect source of nitrous oxide emission (N2O) to the atmosphere, which is the greenhouse gas (GHGs) characterized with the highest global warning potential and acts as a stratospheric ozone depletion agent. Previous investigations performed in the Cretaceous Hesbaye chalk aquifer in Eastern Belgium suggested that the dynamics of N2O in the aquifer is controlled by overlapping biochemical processes such as nitrification and denitrification. The current study aims to obtain better insight concerning the factors controlling the distribution of N2O concentration along a vertical dimension in the aquifer, and to capture and quantify the occurrence of nitrification and denitrification processes in the groundwater system. Low-flow groundwater sampling technique was undertaken at different depths in the aquifer to collect groundwater samples aiming at obtaining information about ambient aquifer hydrogeochemical conditions and their effect on the accumulation of GHGs. Afterwards, laboratory stable isotope experiments, using NO3- and NH4+ compounds labeled with heavy 15N isotope, were applied to quantify the rates of nitrification and denitrification processes. Ambient studies suggest that the occurrence of N transformation was related to denitrification while laboratory incubation experiments did not detect it. Such controversial results might be explained by the discrepancy between real aquifer conditions and lab design studies. Thus, additional in situ tracer experiments should be carried out in areas where natural groundwater fluxes do not flush the injected tracer too rapidly. In addition, it would be useful to conduct microbiological studies to obtain better insight into the nature of subsurface biofilm biotope.", "keywords": ["2. Zero hunger", "Nitrous Oxide", "N stable isotope analysis", "Nitrification", "01 natural sciences", "Low-flow sampling", "6. Clean water", "Calcium Carbonate", "Greenhouse gases", "Belgium", "13. Climate action", "Denitrification", "Laboratories", "Groundwater", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.jconhyd.2021.103797"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Contaminant%20Hydrology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jconhyd.2021.103797", "name": "item", "description": "10.1016/j.jconhyd.2021.103797", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jconhyd.2021.103797"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-08-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2009.12.015", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:50Z", "type": "Journal Article", "created": "2010-01-10", "title": "Interactions Between Residue Placement And Earthworm Ecological Strategy Affect Aggregate Turnover And N2o Dynamics In Agricultural Soil", "description": "Previous laboratory studies using epigeic and anecic earthworms have shown that earthworm activity can considerably increase nitrous oxide (N2O) emissions from crop residues in soils. However, the universality of this effect across earthworm functional groups and its underlying mechanisms remain unclear. The aims of this study were (i) to determine whether earthworms with an endogeic strategy also affect N2O emissions; (ii) to quantify possible interactions with epigeic earthworms; and (iii) to link these effects to earthworm-induced differences in selected soil properties. We initiated a 90-day 15N-tracer mesocosm study with the endogeic earthworm species Aporrectodea caliginosa (Savigny) and the epigeic species Lumbricus rubellus (Hoffmeister). 15N-labeled radish (Raphanus sativus cv. Adagio L.) residue was placed on top or incorporated into the loamy (Fluvaquent) soil. When residue was incorporated, only A. caliginosa significantly (p <0.01) increased cumulative N2O emissions from 1350 to 2223 \u00b5g N2O\u2013N kg-1 soil, with a corresponding increase in the turnover rate of macroaggregates. When residue was applied on top, L. rubellus significantly (p <0.001) increased emissions from 524 to 929 \u00b5g N2O\u2013N kg-1, and a significant (p <0.05) interaction between the two earthworm species increased emissions to 1397 \u00b5g N2O\u2013N kg-1. These effects coincided with an 84% increase in incorporation of residue 15N into the microaggregate fraction by A. caliginosa (p = 0.003) and an 85% increase in incorporation into the macroaggregate fraction by L. rubellus (p = 0.018). Cumulative CO2 fluxes were only significantly increased by earthworm activity (from 473.9 to 593.6 mg CO2\u2013C kg-1 soil; p = 0.037) in the presence of L. rubellus when residue was applied on top. We conclude that earthworm-induced N2O emissions reflect earthworm feeding strategies: epigeic earthworms can increase N2O emissions when residue is applied on top; endogeic earthworms when residue is incorporated into the soil by humans (tillage) or by other earthworm species. The effects of residue placement and earthworm addition are accompanied by changes in aggregate and SOM turnover, possibly controlling carbon, nitrogen and oxygen availability and therefore denitrification. Our results contribute to understanding the important but intricate relations between (functional) soil biodiversity and the soil greenhouse gas balance. Further research should focus on elucidating the links between the observed changes in soil aggregation and controls on denitrification, including the microbial community", "keywords": ["organic-matter dynamics", "2. Zero hunger", "crop residues", "denitrification", "ecosystem engineers", "casts", "no-tillage agroecosystems", "04 agricultural and veterinary sciences", "15. Life on land", "carbon-dioxide", "01 natural sciences", "630", "13. Climate action", "systems", "0401 agriculture", " forestry", " and fisheries", "nitrous-oxide fluxes", "management", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2009.12.015"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2009.12.015", "name": "item", "description": "10.1016/j.soilbio.2009.12.015", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2009.12.015"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-04-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2012.04.019", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:53Z", "type": "Journal Article", "created": "2012-05-03", "title": "Ammonia Volatilization Losses From Surface-Applied Urea With Urease And Nitrification Inhibitors", "description": "Abstract Urease inhibitor (UI) and nitrification inhibitor (NI) have the potential to improve N-use efficiency of applied urea and minimize N losses via gaseous emissions of ammonia (NH3) to the atmosphere and nitrate ( NO 3 \u2212 ) leaching into surface and ground water bodies. There is a growing interest in the formulations of coating chemical fertilizers with both UI and NI. However, limited information is available on the combined use of UI and NI applied with urea fertilizer. Therefore the aim of this study was to investigate the effects of treating urea with both UI and NI to minimize NH3 volatilization. Two experiments were set up in volatilization chambers under controlled conditions to examine this process. In the first experiment, UR was treated with the urease inhibitor NBPT [N-(n-butyl) thiophosphoric acid triamide] at a rate of 1060\u00a0mg\u00a0kg\u22121 urea and/or with the nitrification inhibitor DCD (dicyandiamide) at rates equivalent to 5 or 10% of the urea N. A randomized experimental design with five treatments and five replicates was used: 1) UR, 2) UR\u00a0+\u00a0NBPT, 3) UR\u00a0+\u00a0DCD 10%, 4) UR\u00a0+\u00a0NBPT\u00a0+\u00a0DCD 5%, and 5) UR\u00a0+\u00a0NBPT\u00a0+\u00a0DCD 10%. The fertilizer treatments were applied to the surface of an acidic Red Latosol soil moistened to 60% of the maximum water retention and placed inside volatilization chambers. Controls chambers were added to allow for NH3 volatilized from unfertilized soil or contained in the air that swept over the soil surface. The second experiment had an additional treatment with surface-applied DCD. The chambers were glass vessels (1.5\u00a0L) fit with air inlet and outlet tubings to allow air to pass over the soil. Ammonia volatilized was swept and carried to a flask containing a boric acid solution to trap the gas and then measured daily by titration with a standardized H2SO4 solution. Continuous measurements were recorded for 19 and 23 days for the first and second experiment, respectively. The soil samples were then analyzed for UR\u2013, NH 4 + \u2013 , and NO 3 \u2212 \u2013 N . Losses of NH3 by volatilization with unamended UR ranged from 28 to 37% of the applied N, with peak of losses observed the third day after fertilization. NBPT delayed the peak of NH3 losses due to urease inhibition and reduced NH3 volatilization between 54 and 78% when compared with untreated UR. Up to 10 days after the fertilizer application, NH3 losses had not been affected by DCD in the UR or the UR\u00a0+\u00a0NBPT treatments; thereafter, NH3 volatilization tended to decrease, but not when DCD was present. As a consequence, the addition of DCD caused a 5\u201316% increase in NH3 volatilization losses of the fertilizer N applied as UR from both the UR and the UR\u00a0+\u00a0NBPT treatments. Because the effectiveness of NBPT to inhibit soil urease activity was strong only in the first week, it could be concluded that DCD did not affect the action of NBPT but rather, enhanced volatilization losses by maintaining higher soil NH 4 + concentration and pH for a longer time. Depending on the combination of factors influencing NH3 volatilization, DCD could even offset the beneficial effect of NBPT in reducing NH3 volatilization losses.", "keywords": ["soil chemistry", "Urease inhibitors", "Surface treatment", "nutrient use efficiency", "Ammonia volatilization", "01 natural sciences", "630", "Ammonia", "Oxidation", "DCD", "Urea", "Urea fertilizers", "Fertilizers", "volatilization", "Groundwater", "0105 earth and related environmental sciences", "soil surface", "coating", "fertilizer application", "Urease inhibitor", "04 agricultural and veterinary sciences", "Nitrification inhibitor", "Nitrification", "Inorganic acids", "6. Clean water", "enzyme activity", "inhibitor", "pH effects", "Metabolism", "NBPT", "Denitrification", "Leaching", "Soils", "0401 agriculture", " forestry", " and fisheries", "Experiments", "Stabilized fertilizer"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2012.04.019"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2012.04.019", "name": "item", "description": "10.1016/j.soilbio.2012.04.019", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2012.04.019"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-09-01T00:00:00Z"}}, {"id": "10.1016/j.jenvman.2018.01.064", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:27Z", "type": "Journal Article", "created": "2018-02-05", "title": "Elemental sulfur-based autotrophic denitrification and denitritation: microbially catalyzed sulfur hydrolysis and nitrogen conversions", "description": "The hydrolysis of elemental sulfur (S0) coupled to S0-based denitrification and denitritation was investigated in batch bioassays by microbiological and modeling approaches. In the denitrification experiments, the highest obtained NO3--N removal rate was 20.9\u202fmg/l\u00b7d. In the experiments with the biomass enriched on NO2-, a NO2--N removal rate of 10.7\u202fmg/l\u00b7d was achieved even at a NO2--N concentration as high as 240\u202fmg/l. The Helicobacteraceae family was only observed in the biofilm attached onto the chemically-synthesized S0 particles with a relative abundance up to 37.1%, suggesting it was the hydrolytic biomass capable of S0 solubilization in the novel surface-based model. S0-driven denitrification was modeled as a two-step process in order to explicitly account for the sequential reduction of NO3- to NO2- and then to N2 by denitrifying bacteria.", "keywords": ["Surface-based hydrolysis", "Autotrophic Processes", "Autotrophic denitrification; Autotrophic denitritation; Community structure; Elemental sulfur; Mathematical modeling; Surface-based hydrolysis", "Elemental sulfur", "Nitrates", "Nitrogen", "Hydrolysis", "0211 other engineering and technologies", "02 engineering and technology", "Autotrophic denitrification", "01 natural sciences", "6. Clean water", "Community structure", "Bioreactors", "Autotrophic denitritation", "Denitrification", "Autotrophic denitrification; Autotrophic denitritation; Elemental sulfur; Community structure; Surface-based hydrolysis; Mathematical modeling", "Mathematical modeling", "14. Life underwater", "Sulfur", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/698214/5/anastasiia%20JEMA.pdf"}, {"href": "https://doi.org/10.1016/j.jenvman.2018.01.064"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jenvman.2018.01.064", "name": "item", "description": "10.1016/j.jenvman.2018.01.064", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jenvman.2018.01.064"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-04-01T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2018.12.062", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:29Z", "type": "Journal Article", "created": "2018-12-18", "title": "H2S removal and microbial community composition in an anoxic biotrickling filter under autotrophic and mixotrophic conditions", "description": "Removal of H2S from gas streams using NO3--containing synthetic wastewater was investigated in an anoxic biotrickling filter (BTF) at feed N/S ratios of 1.2-1.7\u2009mol mol-1 with an empty bed residence time of 3.5\u2009min and a hydraulic retention time of 115\u2009min. During 108 days of operation under autotrophic conditions, the BTF showed a maximum elimination capacity (EC) of 19.2\u2009g S m-3\u2009h-1 and H2S removal efficiency (RE) >99%. When the BTF was operated under mixotrophic conditions by adding organic carbon (10.2\u2009g acetate m-3\u2009h-1) to the synthetic wastewater, the H2S EC decreased from 16.4 to 13.1\u2009g S m-3\u2009h-1, while the NO3- EC increased from 9.9 to 11.1\u2009g NO3--N m-3\u2009h-1, respectively. Thiobacillus sp. (98-100% similarity) was the only sulfur-oxidizing nitrate-reducing bacterium detected in the BTF biofilm, while the increased abundance of heterotrophic denitrifiers, i.e. Brevundimonas sp. and Rhodocyclales, increased the N/S ratio during BTF operation. Residence time distribution tests showed that biomass accumulation during BTF operation reduced gas and liquid retention times by 17.1% and 83.5%, respectively.", "keywords": ["570", "Air Pollutants", "Nitrates", "550", "Bacteria", "Microbiota", "116 Chemical sciences", "116", "Waste Disposal", " Fluid", "01 natural sciences", "6. Clean water", "Bioreactors", "Denitrification", "Hydrogen Sulfide", "Filtration", "Water Pollutants", " Chemical", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.jhazmat.2018.12.062"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2018.12.062", "name": "item", "description": "10.1016/j.jhazmat.2018.12.062", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2018.12.062"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2021.150433", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:43Z", "type": "Journal Article", "created": "2021-09-20", "title": "Electrochemical water softening as pretreatment for nitrate electro bioremediation", "description": "Open AccessThe dataset contains the raw data of the figures and tables reported in the open access publication 'Ceballos-Escalera, A., Pous, N., Balaguer, M.D., Puig, S., 2022. Electrochemical water softening as pretreatment for nitrate electro bioremediation. Sci. Total Environ. 806, 150433. https://doi.org/10.1016/J.SCITOTENV.2021.150433'.", "keywords": ["Nitrate-contaminated groundwater; Hardness removal; Denitrifying bioelectrochemical system; Polarity reversal", "Nitrates", "02 engineering and technology", "Bioremediaci\u00f3", "01 natural sciences", "6. Clean water", "12. Responsible consumption", "Bioelectrochemistry", "Biodegradation", " Environmental", "Water Softening", "Aig\u00fces subterr\u00e0nies -- Contaminaci\u00f3", "Denitrification", "Groundwater -- Pollution", "Desnitrificaci\u00f3", "0210 nano-technology", "Groundwater", "Bioremediation", "Water Pollutants", " Chemical", "Bioelectroqu\u00edmica", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2021.150433"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2021.150433", "name": "item", "description": "10.1016/j.scitotenv.2021.150433", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2021.150433"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2015.04.005", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:40Z", "type": "Journal Article", "created": "2015-04-18", "title": "Effects Of Different Biochars And Digestate On N2o Fluxes Under Field Conditions", "description": "Field studies that have investigated the effects of char materials on the emission of nitrous oxide (N2O) are still scarce. Therefore, we conducted a field trial with bio- and hydrochars and measured N2O emissions for one whole year. It was hypothesised that the incorporation of chars reduces the emissions of N2O. Chars were produced by pyrolysis and hydrothermal carbonisation (HTC) using either maize silage or wood residues as feedstock. In addition, after production chars were post-treated with digestate in order to accelerate the ageing process of the chars. Chars and digestate were applied to the soil to raise the C content. Emissions of N2O were measured weekly and soil samples for inorganic nitrogen (N) and soil water-content were taken once a month. Additionally, the abundance of functional marker genes from denitrification (nosZ) was determined in October 2012 and in June 2013. The treatment with pure digestate emitted the most N2O compared to the control and char treatments. However, this was significant only in one case. There were no great differences between the char treatments due to high spatial variability and gene abundance of nosZ did not differ between treatments. Overall, emissions of N2O were relatively low. This was attributed to the heterogeneous distribution of the chars and the sandy soils that did not favour the production of N2O. To conclude, the emissions of N2O were mainly influenced by temperature and precipitation and to a minor extent by the type of char and post-treatment.", "keywords": ["2. Zero hunger", "Air Pollutants", "Nitrous Oxide", "04 agricultural and veterinary sciences", "15. Life on land", "16. Peace & justice", "01 natural sciences", "6. Clean water", "Soil", "Biodegradation", " Environmental", "13. Climate action", "Charcoal", "Denitrification", "0401 agriculture", " forestry", " and fisheries", "Environmental Monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2015.04.005"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2015.04.005", "name": "item", "description": "10.1016/j.scitotenv.2015.04.005", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2015.04.005"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-08-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2024.172054", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:44Z", "type": "Journal Article", "created": "2024-04-02", "title": "Mineral and organic fertilisation influence ammonia oxidisers and denitrifiers and nitrous oxide emissions in a long-term tillage experiment", "description": "Nitrous oxide (N2O) emissions from different agricultural systems have been studied extensively to understand the mechanisms underlying their formation. While a number of long-term field experiments have focused on individual agricultural practices in relation to N2O emissions, studies on the combined effects of multiple practices are lacking. This study evaluated the effect of different tillage [no-till (NT) vs. conventional plough tillage (CT)] in combination with fertilisation [mineral (MIN), compost (ORG), and unfertilised control (CON)] on seasonal N2O emissions and the underlying N-cycling microbial community in one maize growing season. Rainfall events after fertilisation, which resulted in increased soil water content, were the main triggers of the observed N2O emission peaks. The highest cumulative emissions were measured in MIN fertilisation, followed by ORG and CON fertilisation. In the period after the first fertilisation CT resulted in higher cumulative emissions than NT, while no significant effect of tillage was observed cumulatively across the entire season. A higher genetic potential for N2O emissions was observed under NT than CT, as indicated by an increased (nirK\u00a0+\u00a0nirS)/(nosZI\u00a0+\u00a0nosZII) ratio. The mentioned ratio under NT decreased in the order CON > MIN\u00a0>\u00a0ORG, indicating a higher N2O consumption potential in the NT-ORG treatment, which was confirmed in terms of cumulative emissions. The AOB/16S ratio was strongly affected by fertilisation and was higher in the MIN than in the ORG and CON treatments, regardless of the tillage system. Multiple regression has revealed that this ratio is one of the most important variables explaining cumulative N2O emissions, possibly reflecting the role of bacterial ammonia oxidisers in minerally fertilised soil. Although the AOB/16S ratio aligned well with the measured N2O emissions in our experimental field, the higher genetic potential for denitrification expressed by the (nirK\u00a0+\u00a0nirS)/(nosZI\u00a0+\u00a0nosZII) ratio in NT than CT was not realized in the form of increased emissions. Our results suggest that organic fertilisation in combination with NT shows a promising combination for mitigating N2O emissions; however, addressing the yield gap is necessary before incorporating it in recommendations for farmers.", "keywords": ["du\u0161ik", "2. Zero hunger", "compost", "denitrification", "denitrifikacija", "N$_2$O", "kompost", "15. Life on land", "sonaravno kmetijstvo", "nitrifikacija", "nitrification", "6. Clean water", "conservation agriculture", "conservation agriculture", " compost", " N$_2$O", " N-cycle", " nitrification", " denitrification", "N-cycle", "13. Climate action", "ohranitveno kmetijstvo", "ohranitveno kmetijstvo", " sonaravno kmetijstvo", " kompost", " du\u0161ik", " nitrifikacija", " denitrifikacija", "info:eu-repo/classification/udc/631.4"], "contacts": [{"organization": "Govednik, Anton, Eler, Klemen, Miheli\u010d, Rok, Suhadolc, Marjetka,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2024.172054"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2024.172054", "name": "item", "description": "10.1016/j.scitotenv.2024.172054", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2024.172054"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-06-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2006.01.013", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:47Z", "type": "Journal Article", "created": "2006-03-21", "title": "How Important Is N2o Production In Removing Atmospherically Deposited Nitrogen From Uk Moorland Catchments?", "description": "Nitrate (NO3\u2212) leaching due to anthropogenic nitrogen (N) deposition is an environmental problem in many parts of the UK uplands, associated with surface water acidification and affecting lake nutrient balances. It is often assumed that gaseous return of deposited N to the atmosphere as N2O through denitrification may provide an important sink for N. This assumption was tested for four moorland catchments (Allt a\u2019Mharcaidh in the Cairngorms, Afon Gwy in mid-Wales, Scoat Tarn in the English Lake District and River Etherow in the southern Pennines), covering gradients of atmospheric N deposition and surface water NO3\u2212 leaching, through a combination of field and laboratory experiments. Field measurements of N2O fluxes from static chambers with and without additions of NH4NO3 solution were carried out every 4 weeks over 1 yr. Wetted soil cores from the same field plots were used in experimental laboratory incubations at 5 and 15 \u00b0C with and without additions of NH4NO3 solution, followed by measurement of N2O fluxes. Field measurements showed that significant N2O fluxes occurred in only a very small number of plots with most showing zero values for much of the year. The maximum fluxes were 0.24 kg-N/ha/yr from unamended plots at the River Etherow and 0.49 kg-N/ha/yr from plots with NH4NO3 additions at the Allt a\u2019Mharcaidh. Laboratory incubation experiments demonstrated that large N2O fluxes could be induced by warming and NH4NO3 additions, with the top 5 cm of soil cores responsible for the largest fluxes, reaching 11.8 kg-N/ha/yr from a podsol at Scoat Tarn. Acetylene block experiments showed that while N2 was not likely to be a significant denitrification product in these soils, reduced N2O fluxes indicated that nitrification was an important source of N2O in many cases. A simple model of denitrification suggesting that 10\u201380% of net N inputs may be denitrified from non-agricultural soils was found to greatly over-estimate fluxes in the UK uplands. The proportion of deposition denitrified was found to be much closer to the IPCC suggested value of 1% with an upper limit of 10%. Interception of N deposition by vegetation may greatly reduce the net supply of N from this source, while soil acidification or other factors limiting carbon supply to soil microbes may prevent large denitrification fluxes even where NO3\u2212 supply is not limiting.", "keywords": ["denitrification", "nitrous oxide", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "nitrification", "6. Clean water", "moorlands", "nitrogen deposition", "acidification", "13. Climate action", "nitrate leaching", "nitrogen saturation", "0401 agriculture", " forestry", " and fisheries", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2006.01.013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2006.01.013", "name": "item", "description": "10.1016/j.soilbio.2006.01.013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2006.01.013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-08-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2006.09.015", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:48Z", "type": "Journal Article", "created": "2006-10-20", "title": "Do Earthworms Increase N2o Emissions In Ploughed Grassland?", "description": "Abstract Earthworm activity has been reported to lead to increased production of the greenhouse gas nitrous oxide (N2O). This is due to emissions from worms themselves, their casts and drilosphere, as well as to general changes in soil structure. However, it remains to be determined how important this effect is on N2O fluxes from agricultural systems under realistic conditions in terms of earthworm density, soil moisture, tillage activity and residue loads. We quantified the effect of earthworm presence on N2O emissions from a pasture after simulated ploughing of the sod (\u2018grassland renovation\u2019) for different soil moisture contents during a 62-day mesocosm study. Sod (with associated soil) and topsoil were separately collected from a loamy Typic Fluvaquent. Treatments included low (L), medium (M) and high (H) moisture content, in combination with: only soil (S); soil+incorporated sod (SG); soil+incorporated sod+the anecic earthworm Aporrectodea longa (SGE). Nitrous oxide and carbon dioxide (CO2) fluxes were measured for 62\u00a0d. At the end of the incubation period, we determined N2O production under water-saturated conditions, potential denitrification and potential mineralization of the soil after removing the earthworms. Cumulative N2O and CO2 fluxes over 62\u00a0d from incorporated sod were highest for treatment HSGE (973\u00a0\u03bcg N2O-N and 302\u00a0mg CO2-C\u00a0kg\u22121 soil) and lowest for LSG (64\u00a0\u03bcg N2O-N and 188\u00a0mg CO2-C\u00a0kg\u22121 soil). Both cumulative fluxes were significantly different for soil moisture (p", "keywords": ["2. Zero hunger", "denitrification", "casts", "carbon", "permanent pastures", "04 agricultural and veterinary sciences", "15. Life on land", "fluxes", "6. Clean water", "nitrous-oxide emissions", "13. Climate action", "soil organic-matter", "community", "0401 agriculture", " forestry", " and fisheries", "denitrifying bacteria", "management"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2006.09.015"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2006.09.015", "name": "item", "description": "10.1016/j.soilbio.2006.09.015", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2006.09.015"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-02-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2007.03.008", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:48Z", "type": "Journal Article", "created": "2007-04-11", "title": "Earthworm Activity As A Determinant For N2o Emission From Crop Residue", "description": "Earthworm activity may have an effect on nitrous oxide (N2O) emissions from crop residue. However, the importance of this effect and its main controlling variables are largely unknown. The main objective of this study was to determine under which conditions and to what extent earthworm activity impacts N2O emissions from grass residue. For this purpose we initiated a 90-day (experiment I) and a 50-day (experiment II) laboratory mesocosm experiment using a Typic Fluvaquent pasture soil with silt loam texture. In all treatments, residue was applied, and emissions of NO and carbon dioxide (CO2) were measured. In experiment I the residue was applied on top of the soil surface and we tested (a) the effects of the anecic earthworm species Aporrectodea longa (Ude) vs. the epigeic species Lumbricus rubellus (Hoffmeister) and (b) interactions between earthworm activity and bulk density (1.06 vs. 1.61 g cm(-3)). In experiment II we tested the effect of L. rubellus after residue was artificially incorporated in the soil. In experiment 1, N2O emissions in the presence of earthworms significantly increased from 55.7 to 789.1 mu g N2O-N kg(-1) soil (L. rubellus; p <0.001) or to 227.2 mu g N2O-N kg(-1) soil (A. longa, p <0.05). This effect was not dependent on bulk density. However, if the residue was incorporated into the soil (experiment II) the earthworm effect disappeared and emissions were higher (1064.2 mu g N2O-N kg(-1) soil). At the end of the experiment and after removal of earthworms, a drying/wetting and freezing/thawing cycle resulted in significantly higher emissions of NO and CO2 from soil with prior presence of L. rubellus. Soil with prior presence of L. rubellus also had higher potential denitrification. We conclude that the main effect of earthworm activity on N2O emissions is through mixing residue into the soil, switching residue decomposition from an aerobic and low denitrification pathway to one with significant denitrification and NO production. Furthermore, A. longa activity resulted in more stable soil organic matter than L. rubellus. (c) 2007 Elsevier Ltd. All rights reserved.", "keywords": ["2. Zero hunger", "aporrectodea-turgida", "carbon", "octolasion-tyrtaeum", "lumbricus-terrestris", "04 agricultural and veterinary sciences", "denitrification rates", "15. Life on land", "13. Climate action", "nitrous-oxide emission", "soil organic-matter", "0401 agriculture", " forestry", " and fisheries", "agricultural soils", "denitrifying bacteria", "management"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2007.03.008"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2007.03.008", "name": "item", "description": "10.1016/j.soilbio.2007.03.008", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2007.03.008"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-08-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2014.11.012", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:55Z", "type": "Journal Article", "created": "2014-11-29", "title": "Biochar Suppresses N2o Emissions While Maintaining N Availability In A Sandy Loam Soil", "description": "Nitrous oxide (N2O) from agricultural soil is a significant source of greenhouse gas emissions. Biochar amendment can contribute to climate change mitigation by suppressing emissions of N2O from soil, although the mechanisms underlying this effect are poorly understood. We investigated the effect of biochar on soil N2O emissions and N cycling processes by quantifying soil N immobilisation, denitrification, nitrification and mineralisation rates using 15N pool dilution techniques and the FLUAZ numerical calculation model. We then examined whether biochar amendment affected N2O emissions and the availability and transformations of N in soils. Our results show that biochar suppressed cumulative soil N2O production by 91% in near-saturated, fertilised soils. Cumulative denitrification was reduced by 37%, which accounted for 85\u201395 % of soil N2O emissions. We also found that physical/chemical and biological ammonium (NH4+) immobilisation increased with biochar amendment but that nitrate (NO3\u2212) immobilisation decreased. We concluded that this immobilisation was insignificant compared to total soil inorganic N content. In contrast, soil N mineralisation significantly increased by 269% and nitrification by 34% in biochar-amended soil. These findings demonstrate that biochar amendment did not limit inorganic N availability to nitrifiers and denitrifiers, therefore limitations in soil NH4+ and NO3\u2212 supply cannot explain the suppression of N2O emissions. These results support the concept that biochar application to soil could significantly mitigate agricultural N2O emissions through altering N transformations, and underpin efforts to develop climate-friendly agricultural management techniques.", "keywords": ["2. Zero hunger", "Nitrous oxide", "denitrification", "mineralisation", "nitrous oxide", "Mineralisation", "04 agricultural and veterinary sciences", "15. Life on land", "Nitrate", "Nitrification", "01 natural sciences", "nitrification", "6. Clean water", "ammonium", "Biochar", "immobilisation", "nitrate", "13. Climate action", "8. Economic growth", "Denitrification", "Immobilisation", "0401 agriculture", " forestry", " and fisheries", "biochar", "Ammonium", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2014.11.012"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2014.11.012", "name": "item", "description": "10.1016/j.soilbio.2014.11.012", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2014.11.012"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-02-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2015.10.008", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:16:56Z", "type": "Journal Article", "created": "2015-11-10", "title": "Evidence For Denitrification As Main Source Of N2o Emission From Residue-Amended Soil", "description": "Catch crops, especially leguminous catch crops, may increase crop nitrogen (N) supply and decrease environmental impacts in cropping systems, but they may also stimulate nitrous oxide (N2O) emissions following spring incorporation. In this 28-day laboratory incubation study, we examined the carbon (C) and N dynamics and N2O evolution after simulated incorporation of residues from three catch crop species into a loamy sand soil, with variable soil moisture (40, 50 or 60% water-filled pore space (WFPS)). The catch crops include two leguminous (red clover and winter vetch) and one non-leguminous species (ryegrass). Plant material was placed in a discrete layer surrounded by soil in which the nitrate (NO3\u2212) pool was enriched with 15N to distinguish N2O derived from denitrification and nitrification. Net N mineralisation from leguminous catch crops was significant (30\u201348\u00a0mg\u00a0N\u00a0kg\u22121 soil, accounting for 41\u201356% of the added residue-N), whereas ryegrass incorporation resulted in net N immobilisation. The evolution of N2O was probably enhanced by N release from the residues, especially during the second week, which can explain the lower N2O evolution after application of ryegrass. Emission of N2O occurred at all moisture levels, but was higher at 50 and 60% WFPS than at 40% in soil with leguminous residues. The 15N enrichment of N2O indicated that denitrification was the dominant source independent of moisture level and residue type. We conclude that catch crop residues will stimulate N2O emissions via denitrification over a wide range of soil moisture conditions, but that emission levels may depend significantly on residue quality and soil moisture.", "keywords": ["Leguminous cover crop", "2. Zero hunger", "Nitrous oxide", "15N labelling", "Nutrient turnover", "Mineralisation", "04 agricultural and veterinary sciences", "incubation", "15. Life on land", "Air and water emissions", "Pasture and forage crops", "Crop combinations and interactions", "13. Climate action", "Farm nutrient management", "Denitrification", "0401 agriculture", " forestry", " and fisheries", "Incubation"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2015.10.008"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2015.10.008", "name": "item", "description": "10.1016/j.soilbio.2015.10.008", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2015.10.008"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-01-01T00:00:00Z"}}, {"id": "10.1023/a:1004383015778", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:17:22Z", "type": "Journal Article", "title": "The Effect Of Different N Substrates On Biological N2o Production From Forest And Agricultural Light Textured Soils", "description": "N2O emissions from two slightly alkaline sandy soils, from arable land and a woodland, were determined in a laboratory experiment in which the soils were incubated with different sources of nitrogen, with or without glucose, and with 0, 1 and 100 mL C2H2 L-1. Large differences in the rate of N2O production were observed between the two soils and between the different N treatments. The arable soil showed very low N2O emissions derived from reduced forms of N as compared with the N2O which was produced when the soil was provided with NO2- or NO3- and a C source, suggesting a very active denitrifier population. In contrast, the woodland soil showed a very low denitrification activity and a much higher N2O production derived from the oxidation of NH4+ and reduction of NO2- by some processes probably mediated by autotrophic or heterotrophic nitrifiers or dissimilatory NO2- reducers. In both soils, the highest N2O emissions were induced by NO2- addition. Those emissions were demonstrated to have a biological origin, as no significant N2O emissions were measured when the soil was autoclaved.", "keywords": ["Acetylene; Denitrification; N sources; N; 2; O; Nitrification;"]}, "links": [{"href": "https://doi.org/10.1023/a:1004383015778"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1023/a:1004383015778", "name": "item", "description": "10.1023/a:1004383015778", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1023/a:1004383015778"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1998-01-01T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2012.02692.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:18:50Z", "type": "Journal Article", "created": "2012-03-10", "title": "Four Years Of Experimental Climate Change Modifies The Microbial Drivers Of N2o Fluxes In An Upland Grassland Ecosystem", "description": "AbstractEmissions of the trace gas nitrous oxide (N2O) play an important role for the greenhouse effect and stratospheric ozone depletion, but the impacts of climate change on N2O fluxes and the underlying microbial drivers remain unclear. The aim of this study was to determine the effects of sustained climate change on field N2O fluxes and associated microbial enzymatic activities, microbial population abundance and community diversity in an extensively managed, upland grassland. We recorded N2O fluxes, nitrification and denitrification, microbial population size involved in these processes and community structure of nitrite reducers (nirK) in a grassland exposed for 4\uffc2\uffa0years to elevated atmospheric CO2 (+200\uffc2\uffa0ppm), elevated temperature (+3.5\uffc2\uffa0\uffc2\uffb0C) and reduction of summer precipitations (\uffe2\uff88\uff9220%) as part of a long\uffe2\uff80\uff90term, multifactor climate change experiment. Our results showed that both warming and simultaneous application of warming, summer drought and elevated CO2 had a positive effect on N2O fluxes, nitrification, N2O release by denitrification and the population size of N2O reducers and NH4 oxidizers. In situ N2O fluxes showed a stronger correlation with microbial population size under warmed conditions compared with the control site. Specific lineages of nirK denitrifier communities responded significantly to temperature. In addition, nirK community composition showed significant changes in response to drought. Path analysis explained more than 85% of in situ N2O fluxes variance by soil temperature, denitrification activity and specific denitrifying lineages. Overall, our study underlines that climate\uffe2\uff80\uff90induced changes in grassland N2O emissions reflect climate\uffe2\uff80\uff90induced changes in microbial community structure, which in turn modify microbial processes.
", "keywords": ["d\u00e9nitrification", "Biodiversit\u00e9 et Ecologie", "551", "AOB", "diversity", "Biodiversity and Ecology", "nosZ", "[SDV.EE.ECO] Life Sciences [q-bio]/Ecology", " environment/Ecosystems", "nirK", "Milieux et Changements globaux", "2. Zero hunger", "changement climatique", "denitrification", "grasslands", "N2O", "prairie", "04 agricultural and veterinary sciences", "15. Life on land", "nitrification", "6. Clean water", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "climate change", "13. Climate action", "[SDV.EE.ECO]Life Sciences [q-bio]/Ecology", "AOB;changement climatique;d\u00e9nitrification;diversit\u00e9;prairie;N2O;nitrification", "0401 agriculture", " forestry", " and fisheries", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology", "environment/Ecosystems"]}, "links": [{"href": "https://hal.science/halsde-00722571/file/Cantarel_gcb12_1.pdf"}, {"href": "https://doi.org/10.1111/j.1365-2486.2012.02692.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2012.02692.x", "name": "item", "description": "10.1111/j.1365-2486.2012.02692.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2012.02692.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-05-08T00:00:00Z"}}, {"id": "10.1016/j.watres.2020.116748", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:17:13Z", "type": "Journal Article", "created": "2020-12-16", "title": "Electro-bioremediation of nitrate and arsenite polluted groundwater.", "description": "The coexistence of different pollutants in groundwater is a common threat. Sustainable and resilient technologies are required for their treatment. The present study aims to evaluate microbial electrochemical technologies (METs) for treating groundwater contaminated with nitrate (NO3-) while containing arsenic (in form of arsenite (As(III)) as a co-contaminant. The treatment was based on the combination of nitrate reduction to dinitrogen gas and arsenite oxidation to arsenate (exhibiting less toxicity, solubility, and mobility), which can be removed more easily in further post-treatment. We operated a bioelectrochemical reactor at continuous-flow mode with synthetic contaminated groundwater (33 mg N-NO3- L-1 and 5 mg As(III) L-1) identifying the key operational conditions. Different hydraulic retention times (HRT) were evaluated, reaching a maximum nitrate reduction rate of 519 g N-NO3- m3Net Cathodic Compartment d-1 at HRT of 2.3 h with a cathodic coulombic efficiency of around 100 %. Simultaneously, arsenic oxidation was complete at all HRT tested down to 1.6 h reaching an oxidation rate of up to 90 g As(III) m-3Net Reactor Volume d -1. Electrochemical and microbiological characterization of single granules suggested that arsenite at 5 mg L-1 did not have an inhibitory effect on a denitrifying biocathode mainly represented by Sideroxydans sp. Although the coexistence of abiotic and biotic arsenic oxidation pathways was shown to be likely, microbial arsenite oxidation linked to denitrification by Achromobacter sp. was the most probable pathway. This research paves the ground towards a real application for treating groundwater with widespread pollutants.", "keywords": ["Nitrates", "Arsenites", "0211 other engineering and technologies", "02 engineering and technology", "Bioremediaci\u00f3", "01 natural sciences", "6. Clean water", "Arsenic", "12. Responsible consumption", "Bioelectrochemistry", "Biodegradation", " Environmental", "13. Climate action", "Aig\u00fces subterr\u00e0nies -- Contaminaci\u00f3", "Denitrification", "Groundwater -- Pollution", "Desnitrificaci\u00f3", "Groundwater", "Oxidation-Reduction", "Bioremediation", "Water Pollutants", " Chemical", "Bioelectroqu\u00edmica", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.watres.2020.116748"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2020.116748", "name": "item", "description": "10.1016/j.watres.2020.116748", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2020.116748"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-01T00:00:00Z"}}, {"id": "10.1021/acs.est.1c03586", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:17:18Z", "type": "Journal Article", "created": "2021-11-02", "title": "Impact of Periodic Polarization on Groundwater Denitrification in Bioelectrochemical Systems.", "description": "Nitrate contamination is a common problem in groundwater around the world. Nitrate can be cathodically reduced in bioelectrochemical systems using autotrophic denitrifiers with low energy investment and without chemical addition. Successful denitrification was demonstrated in previous studies in both microbial fuel cells and microbial electrolysis cells (MECs) with continuous current flow, whereas the impact of intermittent current supply (e.g., in a fluidized-bed system) on denitrification and particularly the electron-storing capacity of the denitrifying electroactive biofilms (EABs) on the cathodes have not been studied in depth. In this study, two continuously fed MECs were operated in parallel under continuous and periodic polarization modes over 280 days, respectively. Under continuous polarization, the maximum denitrification rate reached 233 g NO3--N/m3/d with 98% nitrate removal (0.6 mg NO3--N/L in the effluent) with negligible intermediate production, while under a 30 s open-circuit/30 s polarization mode, 86% of nitrate was removed at a maximum rate of 205 g NO3--N/m3/d (4.5 mg NO3--N/L in the effluent) with higher N2O production (6.6-9.3 mg N/L in the effluent). Conversely, periodic polarization could be an interesting approach in other bioelectrochemical processes if the generation of chemical intermediates (partially reduced or oxidized) should be favored. Similar microbial communities dominated byGallionellaceaewere found in both MECs; however, swapping the polarization modes and the electrochemical analyses suggested that the periodically polarized EABs probably developed a higher ability for electron storage and transfer, which supported the direct electron transfer pathway in discontinuous operation or fluidized biocathodes.", "keywords": ["bioelectrochemical systems (BESs)", "periodic polarization", "Autotrophic Processes", "Technology and Engineering", "denitrification", "Nitrates", "AUTOTROPHIC DENITRIFICATION", "EABs", "Bioelectric Energy Sources", "NITRATE-CONTAMINATED GROUNDWATER", "02 engineering and technology", "6. Clean water", "REDUCTION", "REMOVAL", "13. Climate action", "Earth and Environmental Sciences", "BACTERIA", "ACETATE", "Denitrification", "MICROBIAL FUEL-CELLS", "ELECTRON-TRANSFER", "BIOFILM", "0210 nano-technology", "Groundwater", "STORAGE"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.1c03586"}, {"href": "https://doi.org/10.1021/acs.est.1c03586"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.est.1c03586", "name": "item", "description": "10.1021/acs.est.1c03586", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.est.1c03586"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-11-02T00:00:00Z"}}, {"id": "10.1023/a:1004518730970", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:17:22Z", "type": "Journal Article", "created": "2002-12-21", "description": "Elevated atmospheric CO2 has the potential to change below-ground nutrient cycling and thereby alter the soil-atmosphere exchange of biogenic trace gases. We measured fluxes of CH4 and N2O in trembling aspen (Populus tremuloides Michx.) stands grown in open-top chambers under ambient and twice-ambient CO2 concentrations crossed with \u2018high\u2019 and low soil-N conditions.", "keywords": ["measurement-", "nitrous-oxide", "flux-", "Vascular-Plants", "poplars-", "carbon-dioxide-enrichment", "photosynthesis-", "Nutrition-", "carbon-dioxide: atmospheric-concentration", "stand-growth", "nitrogen-cycle", "michigan-", "methane-: flux-", "soil-", "nitrogen-", "Populus-tremuloides [aspen-] (Salicaceae-)", "carbon-cycle", "methane-production", "soil-fertility", "Salicaceae-: Dicotyledones-", "populus-tremuloides", "cycling-", "Spermatophytes-", "Spermatophyta-", "Plantae-", "biological-activity-in-soil", "Climatology- (Environmental-Sciences)", "Angiosperms-", "Angiospermae-", "Plants-", "gases-", "oxidation-", "forest-soils", "methane-", "04 agricultural and veterinary sciences", "GLOBAL-ECOLOGY", "15. Life on land", "enzyme-activity", "gas-exchange", "nitrous-oxide: emission-", "soil-water", "13. Climate action", "denitrification-", "0401 agriculture", " forestry", " and fisheries", "soil-bacteria", "Dicots-", "efflux-"]}, "links": [{"href": "https://doi.org/10.1023/a:1004518730970"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1023/a:1004518730970", "name": "item", "description": "10.1023/a:1004518730970", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1023/a:1004518730970"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1999-02-01T00:00:00Z"}}, {"id": "10.1029/2010jg001494", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:17:30Z", "type": "Journal Article", "created": "2011-02-03", "title": "Nitrous Oxide Emissions And Isotopic Composition In Urban And Agricultural Systems In Southern California", "description": "[1]\u00a0Nitrous oxide (N2O) is a powerful greenhouse gas increasing in atmospheric mixing ratio and linked to increasing amounts of reactive N in the environment, particularly fertilizer use in agriculture. The consequences of urbanization of agricultural land for global and regional N2O emissions are unclear, due to high spatial and temporal variability of fluxes from different ecosystems and relatively few studies of urban ecosystems. We measured fluxes and the stable isotope composition (\u03b415N and \u03b418O) of N2O over 1 year in urban (ornamental lawns and athletic fields) and agricultural (corn and vegetable fields) ecosystems near Los Angeles, California, United States. We found that urban landscapes (lawns and athletic fields) have annual N2O fluxes equal to or greater than agricultural fields. Fertilization rates of urban landscapes were equal to or greater than agricultural fields, with comparable N2O emissions factors. \u03b415N and \u03b418O of N2O varied widely in all ecosystems, and were not consistent with ecosystem type, season, soil moisture, or temperature. There was, however, a consistent response of \u03b415N-N2O to pulses of N2O emission following fertilization, with an initial depletion in \u03b415N relative to prefertilization values, then gradual enrichment to background values within about 1 week. Preliminary scaling calculations indicated that N2O emissions from urban landscapes are approximately equal to or greater than agricultural emissions in urbanized areas of southern California, which further implies that current estimates of regional N2O emissions (based on agricultural land area) may be too low.", "keywords": ["2. Zero hunger", "long-term", "denitrification", "variability", "methane", "cycle (with supplement)", "04 agricultural and veterinary sciences", "15. Life on land", "carbon-dioxide", "7. Clean energy", "01 natural sciences", "nitrification", "13. Climate action", "11. Sustainability", "global n2o budget", "soil-moisture", "0401 agriculture", " forestry", " and fisheries", "oxygen-exchange", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://escholarship.org/content/qt7q9586fd/qt7q9586fd.pdf"}, {"href": "https://doi.org/10.1029/2010jg001494"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Geophysical%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1029/2010jg001494", "name": "item", "description": "10.1029/2010jg001494", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1029/2010jg001494"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-02-04T00:00:00Z"}}, {"id": "10.1038/nclimate1692", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:17:36Z", "type": "Journal Article", "created": "2013-01-31", "title": "Greenhouse-gas emissions from soils increased by earthworms", "description": "Earthworms play an essential part in determining the greenhouse-gas balance of soils worldwide but whether their activity moves soils towards being a net source or sink remains controversial. This Review of the overall effect of earthworms on the greenhouse-gas balance of soils suggests that although beneficial to fertility, earthworms tend to increase the net soil emissions of such gases.", "keywords": ["organic-matter dynamics", "2. Zero hunger", "ecosystem engineers", "suelo", "soil fertility", "n2o emission", "earthworms", "04 agricultural and veterinary sciences", "fertilidad del suelo", "endogeic earthworms", "15. Life on land", "carbon-dioxide", "microbial activity", "soil", "12. Responsible consumption", "crop residue", "13. Climate action", "greenhouse gases", "11. Sustainability", "gases de efecto invernadero", "0401 agriculture", " forestry", " and fisheries", "nitrous-oxide fluxes", "agricultural intensification", "nitrifier denitrification", "lombriz de tierra"]}, "links": [{"href": "https://doi.org/10.1038/nclimate1692"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Climate%20Change", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/nclimate1692", "name": "item", "description": "10.1038/nclimate1692", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/nclimate1692"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-02-03T00:00:00Z"}}, {"id": "10.1038/srep01732", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:17:44Z", "type": "Journal Article", "created": "2013-04-25", "title": "Biochar And Denitrification In Soils: When, How Much And Why Does Biochar Reduce N2o Emissions?", "description": "Agricultural soils represent the main source of anthropogenic N2O emissions. Recently, interactions of black carbon with the nitrogen cycle have been recognized and the use of biochar is being investigated as a means to reduce N2O emissions. However, the mechanisms of reduction remain unclear. Here we demonstrate the significant impact of biochar on denitrification, with a consistent decrease in N2O emissions by 10-90% in 14 different agricultural soils. Using the (15)N gas-flux method we observed a consistent reduction of the N2O/(N2 + N2O) ratio, which demonstrates that biochar facilitates the last step of denitrification. Biochar acid buffer capacity was identified as an important aspect for mitigation that was not primarily caused by a pH shift in soil. We propose the function of biochar as an 'electron shuttle' that facilitates the transfer of electrons to soil denitrifying microorganisms, which together with its liming effect would promote the reduction of N2O to N2.", "keywords": ["2. Zero hunger", "Nitrogen", "Nitrous Oxide", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "Article", "Soil", "13. Climate action", "Charcoal", "Denitrification", "0401 agriculture", " forestry", " and fisheries", "Gases", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Cayuela, M.L.*, S\u00e1nchez-Monedero, M.A., Roig, A., Hanley, K., Enders, A., Lehmann, J.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1038/srep01732"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/srep01732", "name": "item", "description": "10.1038/srep01732", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/srep01732"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-04-25T00:00:00Z"}}, {"id": "10.1071/wf10120", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:17:59Z", "type": "Journal Article", "created": "2011-10-16", "title": "Soil N2o Emissions In A Mediterranean Shrubland Disturbed By Experimental Fires", "description": "In the present work, post-burning soil N2O fluxes and related microbial processes were investigated in a Mediterranean shrubland subjected to experimental fires. Nine plots were selected, of which three were used as controls, three were burned with low-intensity fire and three with higher intensity fire. N2O fluxes, soil humidity and temperature were measured starting 2 days before burning and for 1 year after fire. Potential net nitrification, denitrification enzyme activity, mineral N and organic C were measured from soil samples collected periodically after burning. Cumulative data indicate a doubling of N2O production in burned plots over 1 year. Burned plots showed an increase of frequency of hot spots of N2O production. A slight detrimental effect of fire on the analysed biological activities was detected only immediately after burning. After 3 months, both potential net nitrification and denitrification enzyme activity had mostly recovered and potential net nitrification further increased over control levels in the following months. Fire seemed to induce a change in the main source of N2O, which in control plots was represented by heterotrophic activity (50\uffe2\uff80\uff9375%), whereas in burned plots it was mostly of autotrophic origin, most probably due to the significant increase of soil NH4+ after burning.
", "keywords": ["2. Zero hunger", "Nitrous oxide", "denitrification; fire intensity; nitrification; soil nitrogen;", "13. Climate action", "Nitrous oxide; soil; fire", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "fire", "soil"]}, "links": [{"href": "https://doi.org/10.1071/wf10120"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Wildland%20Fire", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1071/wf10120", "name": "item", "description": "10.1071/wf10120", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1071/wf10120"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-01-01T00:00:00Z"}}, {"id": "10.1111/j.1365-2435.2006.01146.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:18:44Z", "type": "Journal Article", "created": "2006-06-29", "title": "Several Components Of Global Change Alter Nitrifying And Denitrifying Activities In An Annual Grassland", "description": "Summary
The effects of global change on below\uffe2\uff80\uff90ground processes of the nitrogen (N) cycle have repercussions for plant communities, productivity and trace gas effluxes. However, the interacting effects of different components of global change on nitrification or denitrification have rarely been studied in situ.
We measured responses of nitrifying enzyme activity (NEA) and denitrifying enzyme activity (DEA) to over 4\uffc2\uffa0years of exposure to several components of global change and their interaction (increased atmospheric CO2 concentration, temperature, precipitation and N addition) at peak biomass period in an annual grassland ecosystem. In order to provide insight into the mechanisms controlling the response of NEA and DEA to global change, we examined the relationships between these activities and soil moisture, microbial biomass C and N, and soil extractable N.
Across all treatment combinations, NEA was decreased by elevated CO2 and increased by N addition. While elevated CO2 had no effect on NEA when not combined with other treatments, it suppressed the positive effect of N addition on NEA in all the treatments that included N addition. We found a significant CO2\uffe2\uff80\uff93N interaction for DEA, with a positive effect of elevated CO2 on DEA only in the treatments that included N addition, suggesting that N limitation of denitrifiers may have occurred in our system. Soil water content, extractable N concentrations and their interaction explained 74% of the variation in DEA.
Our results show that the potentially large and interacting effects of different components of global change should be considered in predicting below\uffe2\uff80\uff90ground N responses of Mediterranean grasslands to future climate changes.
", "keywords": ["ERODIUM BOTRYS", "2. Zero hunger", "N ADDITION", "[SDV.BID]Life Sciences [q-bio]/Biodiversity", "DENITRIFICATION", "04 agricultural and veterinary sciences", "910", "15. Life on land", "NITRIFICATION", "6. Clean water", "BROMUS HORDEACEUS", "GERANIUM DISSECTUM", "GERANIUM", "[SDV.EE] Life Sciences [q-bio]/Ecology", " environment", "13. Climate action", "[SDV.EE]Life Sciences [q-bio]/Ecology", "PRECIPITATION", "WARMING", "0401 agriculture", " forestry", " and fisheries", "AVENA BARBATA", "ELEVATED CO2", "environment", "TENEUR EN EAU DU SOL", "[SDV.BID] Life Sciences [q-bio]/Biodiversity"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2435.2006.01146.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Functional%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2435.2006.01146.x", "name": "item", "description": "10.1111/j.1365-2435.2006.01146.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2435.2006.01146.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-06-29T00:00:00Z"}}, {"id": "10.1093/femsec/fiv066", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:18:17Z", "type": "Journal Article", "created": "2015-06-20", "title": "Effects Of Warming And Drought On Potential N2o Emissions And Denitrifying Bacteria Abundance In Grasslands With Different Land-Use", "description": "Increased warming in spring and prolonged summer drought may alter soil microbial denitrification. We measured potential denitrification activity and denitrifier marker gene abundances (nirK, nirS, nosZ) in grasslands soils in three geographic regions characterized by site-specific land-use indices (LUI) after warming in spring, at an intermediate sampling and after summer drought. Potential denitrification was significantly increased by warming, but did not persist over the intermediate sampling. At the intermediate sampling, the relevance of grassland land-use intensity was reflected by increased potential N2O production at sites with higher LUI. Abundances of total bacteria did not respond to experimental warming or drought treatments, displaying resilience to minor and short-term effects of climate change. In contrast, nirS- and nirK-type denitrifiers were more influenced by drought in combination with LUI and pH, while the nosZ abundance responded to the summer drought manipulation. Land-use was a strong driver for potential denitrification as grasslands with higher LUI also had greater potentials for N2O emissions. We conclude that both warming and drought affected the denitrifying communities and the potential denitrification in grassland soils. However, these effects are overruled by regional and site-specific differences in soil chemical and physical properties which are also related to grassland land-use intensity.", "keywords": ["0301 basic medicine", "570", "UFSP13-8 Global Change and Biodiversity", "Climate Change", "Microbial Consortia", "580 Plants (Botany)", "Nitric Oxide", "142-005 142-005", "Soil", "03 medical and health sciences", "potential N2O emissions", "RNA", " Ribosomal", " 16S", "2402 Applied Microbiology and Biotechnology", "use index", "Soil Microbiology", "2. Zero hunger", "Biodiversity Exploratories", "denitrification", "Bacteria", "2404 Microbiology", "04 agricultural and veterinary sciences", "15. Life on land", "Grassland", "6. Clean water", "Droughts", "land", "climate change", "Genes", " Bacterial", "13. Climate action", "8. Economic growth", "Denitrification", "0401 agriculture", " forestry", " and fisheries", "grassland", "microbial community", "2303 Ecology"]}, "links": [{"href": "https://doi.org/10.1093/femsec/fiv066"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/femsec/fiv066", "name": "item", "description": "10.1093/femsec/fiv066", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/femsec/fiv066"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-06-19T00:00:00Z"}}, {"id": "10.3389/fmicb.2022.859063", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:20:43Z", "type": "Journal Article", "created": "2022-05-17", "title": "Interacting Bioenergetic and Stoichiometric Controls on Microbial Growth", "description": "Microorganisms function as open systems that exchange matter and energy with their surrounding environment. Even though mass (carbon and nutrients) and energy exchanges are tightly linked, there is a lack of integrated approaches that combine these fluxes and explore how they jointly impact microbial growth. Such links are essential to predicting how the growth rate of microorganisms varies, especially when the stoichiometry of carbon- (C) and nitrogen (N)-uptake is not balanced. Here, we present a theoretical framework to quantify the microbial growth rate for conditions of C-, N-, and energy-(co-) limitations. We use this framework to show how the C:N ratio and the degree of reduction of the organic matter (OM), which is also the electron donor, availability of electron acceptors (EAs), and the different sources of N together control the microbial growth rate under C, nutrient, and energy-limited conditions. We show that the growth rate peaks at intermediate values of the degree of reduction of OM under oxic and C-limited conditions, but not under N-limited conditions. Under oxic conditions and with N-poor OM, the growth rate is higher when the inorganic N (NInorg)-source is ammonium compared to nitrate due to the additional energetic cost involved in nitrate reduction. Under anoxic conditions, when nitrate is both EA and NInorg-source, the growth rates of denitrifiers and microbes performing the dissimilatory nitrate reduction to ammonia (DNRA) are determined by both OM degree of reduction and nitrate-availability. Consistent with the data, DNRA is predicted to foster growth under extreme nitrate-limitation and with a reduced OM, whereas denitrifiers are favored as nitrate becomes more available and in the presence of oxidized OM. Furthermore, the growth rate is reduced when catabolism is coupled to low energy yielding EAs (e.g., sulfate) because of the low carbon use efficiency (CUE). However, the low CUE also decreases the nutrient demand for growth, thereby reducing N-limitation. We conclude that bioenergetics provides a useful conceptual framework for explaining growth rates under different metabolisms and multiple resource-limitations.Heterotrophic nitrifiers are able to oxidize and remove ammonia from nitrogen\uffe2\uff80\uff90rich wastewaters but the genetic elements of heterotrophic ammonia oxidation are poorly understood. Here, we isolated and identified a novel heterotrophic nitrifier, Alcaligenes ammonioxydans sp. nov. strain HO\uffe2\uff80\uff901, oxidizing ammonia to hydroxylamine and ending in the production of N2 gas. Genome analysis revealed that strain HO\uffe2\uff80\uff901 encoded a complete denitrification pathway but lacks any genes coding for homologous to known ammonia monooxygenases or hydroxylamine oxidoreductases. Our results demonstrated strain HO\uffe2\uff80\uff901 denitrified nitrite (not nitrate) to N2 and N2O at anaerobic and aerobic conditions respectively. Further experiments demonstrated that inhibition of aerobic denitrification did not stop ammonia oxidation and N2 production. A gene cluster (dnfT1RT2ABCD) was cloned from strain HO\uffe2\uff80\uff901 and enabled E. coli accumulated hydroxylamine. Sub\uffe2\uff80\uff90cloning showed that genetic cluster dnfAB or dnfABC already enabled E. coli cells to produce hydroxylamine and further to 15N2 from (15NH4)2SO4. Transcriptome analysis revealed these three genes dnfA, dnfB and dnfC were significantly upregulated in response to ammonia stimulation. Taken together, we concluded that strain HO\uffe2\uff80\uff901 has a novel dnf genetic cluster for ammonia oxidation and this dnf genetic cluster encoded a previously unknown pathway of direct ammonia oxidation (Dirammox) to N2.
", "keywords": ["Alcaligenes ammonioxydans sp. nov.", "0301 basic medicine", "106014 Genomics", "Nitrogen", "HYDROXYLAMINE OXIDASE", "direct ammonia oxidation (Dirammox)", "OXIDATION", "REDUCTASE", "Water Purification", "THIOSPHAERA-PANTOTROPHA", "PYRUVIC-OXIME", "03 medical and health sciences", "heterotrophic nitrifier", "Ammonia", "106014 Genomik", "Escherichia coli", "Alcaligenes", "wastewater", "Nitrites", "106022 Mikrobiologie", "HETEROTROPHIC NITRIFICATION", "0303 health sciences", "PURIFICATION", "Sewage", "AEROBIC DENITRIFICATION", "Nitrification", "Aerobiosis", "6. Clean water", "NITROGEN", "FAECALIS", "Denitrification", "106022 Microbiology", "Oxidation-Reduction"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15751"}, {"href": "https://doi.org/10.1111/1462-2920.15751"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1462-2920.15751", "name": "item", "description": "10.1111/1462-2920.15751", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1462-2920.15751"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-09-28T00:00:00Z"}}, {"id": "10.1111/1758-2229.13114", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:18:32Z", "type": "Journal Article", "created": "2022-08-09", "title": "N\u2010damo, an opportunity to reduce methane emissions?", "description": "H2020 MGA EJP Cofund, EJP SOIL (TRACE-Soils), Grant/Award Number: 862695; HORIZON CSA, Project PREPSOIL, Grant/Award Number: 101070045", "keywords": ["Highlight", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "15. Life on land", "7. Clean energy", "6. Clean water", "12. Responsible consumption", "03 medical and health sciences", "13. Climate action", "11. Sustainability", "Denitrification", "Anaerobiosis", "Methane", "Oxidation-Reduction", "Nitrites"], "contacts": [{"organization": "G\u00f3mez\u2010Gallego, Tamara", "roles": ["creator"]}]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1758-2229.13114"}, {"href": "https://doi.org/10.1111/1758-2229.13114"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1758-2229.13114", "name": "item", "description": "10.1111/1758-2229.13114", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1758-2229.13114"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-08-09T00:00:00Z"}}, {"id": "10.1111/gcb.16042", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:18:39Z", "type": "Journal Article", "created": "2021-12-19", "title": "Stimulation of ammonia oxidizer and denitrifier abundances by nitrogen loading: Poor predictability for increased soil N2O emission", "description": "AbstractUnprecedented nitrogen (N) inputs into terrestrial ecosystems have profoundly altered soil N cycling. Ammonia oxidizers and denitrifiers are the main producers of nitrous oxide (N2O), but it remains unclear how ammonia oxidizer and denitrifier abundances will respond to N loading and whether their responses can predict N\uffe2\uff80\uff90induced changes in soil N2O emission. By synthesizing 101 field studies worldwide, we showed that N loading significantly increased ammonia oxidizer abundance by 107% and denitrifier abundance by 45%. The increases in both ammonia oxidizer and denitrifier abundances were primarily explained by N loading form, and more specifically, organic N loading had stronger effects on their abundances than mineral N loading. Nitrogen loading increased soil N2O emission by 261%, whereas there was no clear relationship between changes in soil N2O emission and shifts in ammonia oxidizer and denitrifier abundances. Our field\uffe2\uff80\uff90based results challenge the laboratory\uffe2\uff80\uff90based hypothesis that increased ammonia oxidizer and denitrifier abundances by N loading would directly cause higher soil N2O emission. Instead, key abiotic factors (mean annual precipitation, soil pH, soil C:N ratio, and ecosystem type) explained N\uffe2\uff80\uff90induced changes in soil N2O emission. Altogether, these findings highlight the need for considering the roles of key abiotic factors in regulating soil N transformations under N loading to better understand the microbially mediated soil N2O emission.Long\uffe2\uff80\uff90term effects of elevated atmospheric CO2 on the ammonia\uffe2\uff80\uff90oxidizing and denitrifying bacteria in a grassland soil were investigated to test whether a shift in abundance of these N\uffe2\uff80\uff90cycling microorganisms was responsible for enhanced N2O emissions under elevated atmospheric CO2. Soil samples (7.5\uffc2\uffa0cm increments to 45\uffc2\uffa0cm depth) were collected in 2008 from the University of Giessen Free Air Carbon dioxide Enrichment (GiFACE), a permanent grassland exposed to moderately elevated atmospheric CO2 (+20%) since 1998. GiFACE plots lay on a soil moisture gradient because of gradually changing depth to the underlying water table and labeled as the DRY block (furthest from water table), MED block (intermediate to water table), and WET block (nearest to water table). Mean N2O emissions measured since 1998 have been significantly higher under elevated CO2. This study sought to identify microbial and biochemical parameters that might explain higher N2O emissions under elevated CO2. Soil biochemical parameters [extractable organic carbon (EOC), dissolved organic nitrogen (DON), NH4+, NO3\uffe2\uff88\uff92], and abundances of genes encoding the key enzymes involved in ammonia oxidation (amoA) and denitrification (nirK, nirS, nosZ) depended more on soil depth and block (underlying soil moisture gradient) than on elevated CO2. Ammonia oxidation and denitrification gene abundances, relative abundances (ratios) of nirS to nirK, of nosZ to both nirS and to nirK, and of the measured soil biochemical properties DON and NO3\uffe2\uff88\uff92 tended to be lower in elevated CO2 plots as compared with ambient plots in the MED and WET blocks while the DRY block exhibited an opposite trend. High N2O emissions under elevated CO2 in the MED and WET blocks correlated with lower nosZ to nirK ratios, suggesting that increased N2O emissions under elevated CO2 might be caused by a higher proportion of N2O\uffe2\uff80\uff90producing rather than N2O consuming (N2 producing) denitrifiers.
", "keywords": ["nirS", "2. Zero hunger", "N2O emissions", "denitrification", "[SDE.MCG]Environmental Sciences/Global Changes", "04 agricultural and veterinary sciences", "15. Life on land", "AOA", "6. Clean water", "AOB", "soil", "Enrichissement en gaz carbonique", "[SDE.MCG] Environmental Sciences/Global Changes", "Concentration \u00e9lev\u00e9e en CO2", "nosZ", "FACE", "13. Climate action", "ammonia oxidation", "nirK", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2486.2011.02470.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2011.02470.x", "name": "item", "description": "10.1111/j.1365-2486.2011.02470.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2011.02470.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-07-10T00:00:00Z"}}, {"id": "10.1371/journal.pone.0102315", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:19:27Z", "type": "Journal Article", "created": "2014-07-16", "title": "Soil Biochemical Responses To Nitrogen Addition In A Bamboo Forest", "description": "Many vital ecosystem processes take place in the soils and are greatly affected by the increasing active nitrogen (N) deposition observed globally. Nitrogen deposition generally affects ecosystem processes through the changes in soil biochemical properties such as soil nutrient availability, microbial properties and enzyme activities. In order to evaluate the soil biochemical responses to elevated atmospheric N deposition in bamboo forest ecosystems, a two-year field N addition experiment in a hybrid bamboo (Bambusa pervariabilis \u00d7 Dendrocalamopsis daii) plantation was conducted. Four levels of N treatment were applied: (1) control (CK, without N added), (2) low-nitrogen (LN, 50 kg N ha(-1) year(-1)), (3) medium-nitrogen (MN, 150 kg N ha(-1) year(-1)), and (4) high-nitrogen (HN, 300 kg N ha(-1) year(-1)). Results indicated that N addition significantly increased the concentrations of NH4(+), NO3(-), microbial biomass carbon, microbial biomass N, the rates of nitrification and denitrification; significantly decreased soil pH and the concentration of available phosphorus, and had no effect on the total organic carbon and total N concentration in the 0-20 cm soil depth. Nitrogen addition significantly stimulated activities of hydrolytic enzyme that acquiring N (urease) and phosphorus (acid phosphatase) and depressed the oxidative enzymes (phenol oxidase, peroxidase and catalase) activities. Results suggest that (1) this bamboo forest ecosystem is moving towards being limited by P or co-limited by P under elevated N deposition, (2) the expected progressive increases in N deposition may have a potential important effect on forest litter decomposition due to the interaction of inorganic N and oxidative enzyme activities, in such bamboo forests under high levels of ambient N deposition.", "keywords": ["Analysis of Variance", "China", "Atmosphere", "Monophenol Monooxygenase", "Nitrogen", "Science", "Q", "Acid Phosphatase", "R", "04 agricultural and veterinary sciences", "Forests", "15. Life on land", "Catalase", "Nitrification", "Soil", "Ammonia", "Denitrification", "Medicine", "Urea", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Sasa", "Soil Microbiology", "Research Article", "Peroxidase"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0102315"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0102315", "name": "item", "description": "10.1371/journal.pone.0102315", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0102315"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-07-16T00:00:00Z"}}, {"id": "10.1128/aem.00033-11", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-25T16:19:08Z", "type": "Journal Article", "created": "2011-04-23", "title": "Association Of Earthworm-Denitrifier Interactions With Increased Emission Of Nitrous Oxide From Soil Mesocosms Amended With Crop Residue", "description": "ABSTRACTEarthworm activity is known to increase emissions of nitrous oxide (N 2 O) from arable soils. Earthworm gut, casts, and burrows have exhibited higher denitrification activities than the bulk soil, implicating priming of denitrifying organisms as a possible mechanism for this effect. Furthermore, the earthworm feeding strategy may drive N 2 O emissions, as it determines access to fresh organic matter for denitrification. Here, we determined whether interactions between earthworm feeding strategy and the soil denitrifier community can predict N 2 O emissions from the soil. We set up a 90-day mesocosm experiment in which 15 N-labeled maize ( Zea mays L.) was either mixed in or applied on top of the soil in the presence or absence of the epigeic earthworm Lumbricus rubellus and/or the endogeic earthworm Aporrectodea caliginosa . We measured N 2 O fluxes and tested the bulk soil for denitrification enzyme activity and the abundance of 16S rRNA and denitrifier genes nirS and nosZ through real-time quantitative PCR. Compared to the control, L. rubellus increased denitrification enzyme activity and N 2 O emissions on days 21 and 90 (day 21, P = 0.034 and P = 0.002, respectively; day 90, P = 0.001 and P = 0.007, respectively), as well as cumulative N 2 O emissions (76%; P = 0.014). A. caliginosa activity led to a transient increase of N 2 O emissions on days 8 to 18 of the experiment. Abundance of nosZ was significantly increased (100%) on day 90 in the treatment mixture containing L. rubellus alone. We conclude that L. rubellus increased cumulative N 2 O emissions by affecting denitrifier community activity via incorporation of fresh residue into the soil and supplying a steady, labile carbon source.
", "keywords": ["2. Zero hunger", "agricultural soil", "Bacteria", "nosz genes", "carbon", "Nitrous Oxide", "n2o emission", "n2o-producing microorganisms", "04 agricultural and veterinary sciences", "15. Life on land", "pcr data", "microbial activity", "Animal Feed", "Zea mays", "lumbricus-rubellus", "Soil", "Denitrification", "Animals", "0401 agriculture", " forestry", " and fisheries", "community composition", "Oligochaeta", "organic-matter"]}, "links": [{"href": "https://doi.org/10.1128/aem.00033-11"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20and%20Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/aem.00033-11", "name": "item", "description": "10.1128/aem.00033-11", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/aem.00033-11"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-06-15T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Denitrification&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Denitrification&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Denitrification&", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Denitrification&offset=50", "hreflang": "en-US"}], "numberMatched": 76, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-05-26T09:11:31.809927Z"}