{"type": "FeatureCollection", "features": [{"id": "10.1186/s40064-016-2587-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:17:56Z", "type": "Journal Article", "created": "2016-08-02", "title": "Contrasting Effects Of Exogenous Phosphorus Application On N2o Emissions From Two Tropical Forest Soils With Contrasting Phosphorus Availability", "description": "An incubation study was conducted to test the effects of phosphorus (P) addition on nitrous oxide (N2O) emissions from the soils taken from two tropical rain forests established on different parent materials [meta-sedimentary (MS) and ultrabasic (UB) rock] on Mt. Kinabalu, Borneo. Earlier studies suggest that the forest on UB soils is more strongly limited by P than that on MS soils is. In MS soils, P addition significantly reduced N2O emissions. Since neither ammonium (NH4 (+)) nor nitrate (NO3 (-)) contents were reduced by P addition, we assumed that the decrease in N2O emissions were not due to the previously-reported mechanism: P addition stimulated microbial nitrogen (N) immobilization and collateral inorganic N consumption, reducing resources for producing N2O. Since P addition enhanced the ratios of microbial biomass to CO2 and N2O emissions (indicators of nitrifying and/or denitrifying respiratory efficiency), it was suggested that the N required for the respiration of nitrifying and/or denitrifying bacteria was reduced, leading to reduced N2O emissions. On the other hand, P addition had no effects on N2O emissions in UB soils. The respiratory efficiency did not change significantly by P addition, possibly because the microbial community in the highly-P-depleted UB soils shifted by P addition, with which the enhancement of respiration efficiency did not co-vary. We concluded that (1) P addition may control N2O emissions through increasing respiratory efficiency, and (2) the effects may be different depending on the differences in P availability.", "keywords": ["Nitrous oxide", "13. Climate action", "Research", "Denitrification", "Tropics", "0401 agriculture", " forestry", " and fisheries", "Phosphorus limitation", "04 agricultural and veterinary sciences", "15. Life on land", "Nitrification"]}, "links": [{"href": "https://doi.org/10.1186/s40064-016-2587-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/SpringerPlus", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s40064-016-2587-5", "name": "item", "description": "10.1186/s40064-016-2587-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s40064-016-2587-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-08-02T00:00:00Z"}}, {"id": "10.1371/journal.pone.0102315", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:18:03Z", "type": "Journal Article", "created": "2014-07-16", "title": "Soil Biochemical Responses To Nitrogen Addition In A Bamboo Forest", "description": "Many vital ecosystem processes take place in the soils and are greatly affected by the increasing active nitrogen (N) deposition observed globally. Nitrogen deposition generally affects ecosystem processes through the changes in soil biochemical properties such as soil nutrient availability, microbial properties and enzyme activities. In order to evaluate the soil biochemical responses to elevated atmospheric N deposition in bamboo forest ecosystems, a two-year field N addition experiment in a hybrid bamboo (Bambusa pervariabilis \u00d7 Dendrocalamopsis daii) plantation was conducted. Four levels of N treatment were applied: (1) control (CK, without N added), (2) low-nitrogen (LN, 50 kg N ha(-1) year(-1)), (3) medium-nitrogen (MN, 150 kg N ha(-1) year(-1)), and (4) high-nitrogen (HN, 300 kg N ha(-1) year(-1)). Results indicated that N addition significantly increased the concentrations of NH4(+), NO3(-), microbial biomass carbon, microbial biomass N, the rates of nitrification and denitrification; significantly decreased soil pH and the concentration of available phosphorus, and had no effect on the total organic carbon and total N concentration in the 0-20 cm soil depth. Nitrogen addition significantly stimulated activities of hydrolytic enzyme that acquiring N (urease) and phosphorus (acid phosphatase) and depressed the oxidative enzymes (phenol oxidase, peroxidase and catalase) activities. Results suggest that (1) this bamboo forest ecosystem is moving towards being limited by P or co-limited by P under elevated N deposition, (2) the expected progressive increases in N deposition may have a potential important effect on forest litter decomposition due to the interaction of inorganic N and oxidative enzyme activities, in such bamboo forests under high levels of ambient N deposition.", "keywords": ["Analysis of Variance", "China", "Atmosphere", "Monophenol Monooxygenase", "Nitrogen", "Science", "Q", "Acid Phosphatase", "R", "04 agricultural and veterinary sciences", "Forests", "15. Life on land", "Catalase", "Nitrification", "Soil", "Ammonia", "Denitrification", "Medicine", "Urea", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Sasa", "Soil Microbiology", "Research Article", "Peroxidase"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0102315"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0102315", "name": "item", "description": "10.1371/journal.pone.0102315", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0102315"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-07-16T00:00:00Z"}}, {"id": "10.1371/journal.pone.0219479", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:18:04Z", "type": "Journal Article", "created": "2019-07-23", "title": "An integrated assessment of nitrogen source, transformation and fate within an intensive dairy system to inform management change", "description": "From an environmental perspective optimised dairy systems, which follow current regulations, still have low nitrogen (N) use efficiency, high N surplus (kg N ha-1) and enable ad-hoc delivery of direct and indirect reactive N losses to water and the atmosphere. The objective of the present study was to divide an intensive dairy farm into N attenuation capacity areas based on this ad-hoc delivery. Historical and current spatial and temporal multi-level data- sets (stable isotope and dissolved gas) were combined and interpreted. Results showed that the farm had four distinct attenuation areas: high N attenuation: characterised by ammonium-N (NH4+-N) below 0.23 mg NH4+-N l-1 and nitrate (NO3--N) below 5.65 mg NO3-- N l-1 in surface, drainage and groundwater, located on imperfectly to moderately-well drained soils with high denitrification potential and low nitrous oxide (N2O) emissions (av. 0.0032 mg N2O-N l-1); moderate N attenuation: characterised by low NO3--N concentration in drainage water but high N2O production (0.0317 mg N2O-N l-1) and denitrification potential lower than group 1 (av. \u03b415N-NO3-: 16.4 , av. \u03b418O-NO3-: 9.2 ), on well to moderately drained soils; low N attenuation area 1: characterised by high NO3--N (av. 6.90 mg NO3--N l-1) in drainage water from well to moderately-well drained soils, with low denitrification potential (av. \u03b415N-NO3-: 9.5 , av. \u03b418O-NO3-: 5.9 ) and high N2O emissions (0.0319 mg N2O l-1); and low N attenuation area 2: characterised by high NH4+-N (av. 3.93 mg NH4+-N l-1 and high N2O emissions (av. 0.0521 mg N2O l-1) from well to imperfectly drained soil. N loads on site should be moved away from low attenuation areas and emissions to air and water should be assessed.", "keywords": ["dairy systems", "Farms", "Time Factors", "550", "Nitrogen", "Science", "Nitrous Oxide", "management change", "Oxygen Isotopes", "01 natural sciences", "Permeability", "nitrogen", "dissimilatory nitrate reduction to ammonium", "soil", "Dairy system", "Soil", "Isotopes", "Waste Management", "Oxygen Radioisotopes", "Ammonium Compounds", "0105 earth and related environmental sciences", "2. Zero hunger", "Nitrates", "Geography", "Stable Isotopes", "Q", "R", "Water", "Agriculture", "15. Life on land", "nitrification", "6. Clean water", "Management", "DNRA", "Dairying", "Milk", "Slurries", "13. Climate action", "Denitrification", "Medicine", "Intensive", "Research Article"]}, "links": [{"href": "https://eprints.whiterose.ac.uk/148308/8/journal.pone.0219479.pdf"}, {"href": "https://air.unimi.it/bitstream/2434/956826/2/document.pdf"}, {"href": "https://doi.org/10.1371/journal.pone.0219479"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0219479", "name": "item", "description": "10.1371/journal.pone.0219479", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0219479"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-07-23T00:00:00Z"}}, {"id": "10.1890/06-2113.1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:18:20Z", "type": "Journal Article", "created": "2007-12-12", "title": "Effects Of Restoration And Reflooding On Soil Denitrification In A Leveed Midwestern Floodplain", "description": "

River floodplains have the potential to remove nitrate from water through denitrification, the anaerobic microbial conversion of nitrate to nitrogen gas. An important factor in this process is the interaction of river water with floodplain soil; however, many rivers have been disconnected from their historic floodplains by levees. To test the effect of reflooding a degraded floodplain on nitrate removal, we studied changes in soil denitrification rates on the Baraboo River floodplain in Wisconsin, USA, as it underwent restoration. Prior to this study, the site had been leveed, drained, and farmed for more than 50 years. In late fall 2002, the field drainage system was removed, and a gate structure was installed to allow controlled flooding of this site with river water. Soil moisture was extremely variable among zones and months and reflected local weather. Soil organic matter was stable over the study period with differences occurring along the elevation gradient. High soil nitrate concentrations occurred in dry, relatively organic\uffe2\uff80\uff90poor soil samples and, conversely, all samples with high moisture soils characterized by low nitrate. We measured denitrification in static cores and potential denitrification in bulk samples amended with carbon and nitrogen, one year before and two years following the manipulation. Denitrification rates showed high temporal and spatial variability. Static core rates of individual sites ranged widely (from 0.00 to 16.7 \uffce\uffbcg N2O\uffe2\uff80\uff90N\uffc2\uffb7[kg soil]\uffe2\uff88\uff921\uffc2\uffb7h\uffe2\uff88\uff921, mean \uffc2\uffb1 SD = 1.10 \uffc2\uffb1 3.02), and denitrification enzyme activity (DEA) rates were similar with a slightly higher mean (from 0.00 to 15.0 \uffce\uffbcg N2O\uffe2\uff80\uff90N\uffc2\uffb7[kg soil]\uffe2\uff88\uff921\uffc2\uffb7h\uffe2\uff88\uff921, 1.41 \uffc2\uffb1 1.98). Denitrification was not well\uffe2\uff80\uff90correlated with soil nitrate, organic matter content, or moisture levels, the three parameters typically thought to control denitrification. Static core denitrification rates were not significantly different across years, and DEA rates decreased slightly the second year after restoration. These results demonstrate that restored agricultural soil has the potential for denitrification, but that floodplain restoration did not immediately improve this potential. Future floodplain restorations should be designed to test alternative methods of increasing denitrification.

", "keywords": ["floodplain", "2. Zero hunger", "Conservation of Natural Resources", "Time Factors", "Nitrogen", "riparian", "Water", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "nitrogen", "wetland", "6. Clean water", "Midwestern United States", "Disasters", "Soil", "soil denitrification", "0401 agriculture", " forestry", " and fisheries", "river restoration", "Environmental Sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1890/06-2113.1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecological%20Applications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1890/06-2113.1", "name": "item", "description": "10.1890/06-2113.1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1890/06-2113.1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-12-01T00:00:00Z"}}, {"id": "10.2134/jeq2017.03.0106", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:18:32Z", "type": "Journal Article", "created": "2017-08-03", "title": "Combining Urease And Nitrification Inhibitors With Incorporation Reduces Ammonia And Nitrous Oxide Emissions And Increases Corn Yields", "description": "

Less than 50% of applied nitrogen (N) fertilizer is typically recovered by corn (Zea mays L.) due to climatic constraints, soil degradation, overapplication, and losses to air and water. Two application methods, two N sources, and two inhibitors were evaluated to reduce N losses and enhance crop uptake. The treatments included broadcast urea (BrUrea), BrUrea with a urease inhibitor (BrUrea+UI), BrUrea with a urease and a nitrification inhibitor (BrUrea+UI+NI), injection of urea ammonium nitrate (InjUAN), and injected with one or both inhibitors (InjUAN+UI, InjUAN+UI+NI), and a control. The BrUrea treatment lost 50% (64.4 kg N ha\uffe2\uff88\uff921) of the applied N due to ammonia volatilization, but losses were reduced by 64% with BrUrea+UI+NI (23.0 kg N ha\uffe2\uff88\uff921) and by 60% with InjUAN (26.1 kg N ha\uffe2\uff88\uff921). Ammonia losses were lower and crop yields were greater in 2014 than 2013 as a result of the more favorable weather when N was applied in 2014. When ammonia volatilization was reduced by adding a urease inhibitor, N2O emissions were increased by 30 to 31% with BrUrea+UI and InjUAN+UI compared with BrUrea and InjUAN, respectively. Pollution swapping was avoided when both inhibitors were used (BrUrea+UI+NI, InjUAN+UI+NI) as both ammonia volatilization and N2O emissions were reduced, and corn grain yields increased by 5% with BrUrea+UI+NI and by 7% with InjUAN+UI+NI compared with BrUrea and InjUAN, respectively. The combination of two N management strategies (InjUAN+UI+NI) increased yields by 19% (12.9 t ha\uffe2\uff88\uff921) compared with BrUrea (10.8 t ha\uffe2\uff88\uff921).

Core Ideas

Ammonia volatilization resulted in 50% loss of applied urea over 2 yr.

When urease inhibitors were added with urea, ammonia volatilization was reduced by 64%.

Injection of UAN reduced ammonia volatilization by 60% compared with broadcast urea.

N2O emissions were increased by 30 to 31% when urease inhibitors were applied.

Pollution swapping was avoided when both urease and nitrification inhibitors were used.

", "keywords": ["2. Zero hunger", "Ammonia", "13. Climate action", "Denitrification", "Nitrous Oxide", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "Fertilizers", "Nitrification", "Urease", "Zea mays", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.2134/jeq2017.03.0106"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2017.03.0106", "name": "item", "description": "10.2134/jeq2017.03.0106", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2017.03.0106"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-09-01T00:00:00Z"}}, {"id": "10.2166/wst.2018.398", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:18:42Z", "type": "Journal Article", "created": "2018-10-04", "title": "Sensitivity analysis for an elemental sulfur-based two-step denitrification model", "description": "Abstract

A local sensitivity analysis was performed for a chemically synthesized elemental sulfur (S0)-based two-step denitrification model, accounting for nitrite (NO2\uffe2\uff88\uff92) accumulation, biomass growth and S0 hydrolysis. The sensitivity analysis was aimed at verifying the model stability, understanding the model structure and individuating the model parameters to be further optimized. The mass specific area of the sulfur particles (a*) and hydrolysis kinetic constant (k1) were identified as the dominant parameters on the model outputs, i.e. nitrate (NO3\uffe2\uff88\uff92), NO2\uffe2\uff88\uff92 and sulfate (SO42\uffe2\uff88\uff92) concentrations, confirming that the microbially catalyzed S0 hydrolysis is the rate-limiting step during S0-driven denitrification. Additionally, the maximum growth rates of the denitrifying biomass on NO3\uffe2\uff88\uff92 and NO2\uffe2\uff88\uff92 were detected as the most sensitive kinetic parameters.

", "keywords": ["Elemental sulfur", "Environmental Engineering", "0207 environmental engineering", "Biological surface-based hydrolysis; Elemental sulfur; Mathematical modeling; Sensitivity analysis; Two-step autotrophic denitrification; Environmental Engineering; Water Science and Technology", "02 engineering and technology", "01 natural sciences", "Two-step autotrophic denitrification", "Bioreactors", "European Joint Doctorates", "European Commission", "Knowmad Institut", "Biological surface-based hydrolysis", "Nitrites", "Netherlands", "Water Science and Technology", "0105 earth and related environmental sciences", "Aurora Universities Network", "EC", "Nitrates", "H2020", "Energy Research", "13. Climate action", "Denitrification", "Mathematical modeling", "Sensitivity analysis", "Sulfur"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/724909/2/2018%20-%20Kostrytsia%20et%20al.%20-%20Water%20Science%20%26%20Technology%20-%20Sensitivity%20analysis%20for%20S0-based%20denitrification%20model.pdf"}, {"href": "http://iwaponline.com/wst/article-pdf/78/6/1296/504647/wst078061296.pdf"}, {"href": "https://doi.org/10.2166/wst.2018.398"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Science%20and%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2166/wst.2018.398", "name": "item", "description": "10.2166/wst.2018.398", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2166/wst.2018.398"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-09-20T00:00:00Z"}}, {"id": "10.2166/wst.2022.179", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:18:42Z", "type": "Journal Article", "created": "2022-06-01", "title": "Comparison of simple models for total nitrogen removal from agricultural runoff in FWS wetlands", "description": "Abstract

Free water surface (FWS) wetlands can be used to treat agricultural runoff, thereby reducing diffuse pollution. However, as these are highly dynamic systems, their design is still challenging. Complex models tend to require detailed information for calibration, which can only be obtained when the wetland is constructed. Hence simplified models are widely used for FWS wetlands design. The limitations of these models in full-scale FWS wetlands is that these systems often cope with stochastic events with different input concentrations. In our study, we compared different simple transport and degradation models for total nitrogen under steady- and unsteady-state conditions using information collected from a tracer experiment and data from two precipitation events from a full-scale FWS wetland. The tanks-in-series model proved to be robust for simulating solute transport, and the first-order degradation model with non-zero background concentration performed best for total nitrogen concentrations. However, the optimal background concentration changed from event to event. Thus, to use the model as a design tool, it is advisable to include an upper and lower background concentration to determine a range of wetland performance under different events. Models under steady- and unsteady-state conditions with simulated data showed good performance, demonstrating their potential for wetland design.

", "keywords": ["agricultural runoff", " design models", " free water surface wetlands", " modelling", " treatment wetlands", "Nitrogen", "treatment wetlands", "0207 environmental engineering", "Water", "02 engineering and technology", "15. Life on land", "Environmental technology. Sanitary engineering", "01 natural sciences", "agricultural runoff", "6. Clean water", "Water Purification", "modelling", "13. Climate action", "Wetlands", "Denitrification", "design models", "free water surface wetlands", "TD1-1066", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://cris.unibo.it/bitstream/11585/889925/1/wst085113301.pdf"}, {"href": "https://iwaponline.com/wst/article-pdf/85/11/3301/1062302/wst085113301.pdf"}, {"href": "https://doi.org/10.2166/wst.2022.179"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Science%20and%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2166/wst.2022.179", "name": "item", "description": "10.2166/wst.2022.179", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2166/wst.2022.179"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-06-01T00:00:00Z"}}, {"id": "10.3389/fenvs.2014.00025", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:18:52Z", "type": "Journal Article", "created": "2014-07-03", "description": "Open AccessPeer reviewed", "keywords": ["2. Zero hunger", "nitrous oxide", "nitrifier-denitrification", "codenitrification", "Nitrous Oxide", "04 agricultural and veterinary sciences", "15. Life on land", "nitrification", "6. Clean water", "Environmental sciences", "nitrogen fertilizers", "13. Climate action", "nitrous oxide", " charcoal", " nitrification", " DCD", " codenitrification", " nitrogen fertilizers", "Charcoal", "Environmental Science", "DCD", "0401 agriculture", " forestry", " and fisheries", "GE1-350", "charcoal", "Nitrogen fertilizers"], "contacts": [{"organization": "S\u00e1nchez-Garc\u00eda, Mar\u00eda, Roig, Asunci\u00f3n, S\u00e1nchez-Monedero, Miguel \u00c1ngel, Cayuela, Mar\u00eda Luz,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.3389/fenvs.2014.00025"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Environmental%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fenvs.2014.00025", "name": "item", "description": "10.3389/fenvs.2014.00025", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fenvs.2014.00025"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-07-03T00:00:00Z"}}, {"id": "10.3390/w10030287", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:19:11Z", "type": "Journal Article", "created": "2018-03-08", "title": "Effectiveness of a Natural Headwater Wetland for Reducing Agricultural Nitrogen Loads", "description": "

Natural wetlands can play a key role in controlling non-point source pollution, but quantifying their capacity to reduce contaminant loads is often challenging due to diffuse and variable inflows. The nitrogen removal performance of a small natural headwater wetland in a pastoral agricultural catchment in Waikato, New Zealand was assessed over a two-year period (2011\uffe2\uff80\uff932013). Flow and water quality samples were collected at the wetland upper and lower locations, and piezometers sampled inside and outside the wetland. A simple dynamic model operating on an hourly time step was used to assess wetland removal performance for key N species. Hourly measurements of inflow, outflow, rainfall and Penman-Monteith evapotranspiration estimates were used to calculate dynamic water balance for the wetland. A dynamic N mass balance was calculated for each N component by coupling influent concentrations to the dynamic water balance and applying a first order areal removal coefficient (k20) adjusted to the ambient temperature. Flow and water quality monitoring showed that wetland was mainly groundwater fed. The concentrations of oxidised nitrogen (NOx-N, Total Organic Nitrogen (TON) and Total-N (TN) were lower at the outlet of the wetland regardless of flow conditions or seasonality, even during winter storms. The model estimation showed that the wetland could reduce net NOx-N, NH4-N, TON and TN loads by 76%, 73%, 26% and 57%, respectively.

", "keywords": ["wetland attenuation; nitrogen; nutrient removal; denitrification; modelling; agricultural pollution", "13. Climate action", "15. Life on land", "01 natural sciences", "6. Clean water", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://www.mdpi.com/2073-4441/10/3/287/pdf"}, {"href": "https://doi.org/10.3390/w10030287"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/w10030287", "name": "item", "description": "10.3390/w10030287", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/w10030287"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-03-08T00:00:00Z"}}, {"id": "10451/51022", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:21:42Z", "type": "Journal Article", "created": "2017-10-10", "title": "Nitric Oxide Accumulation: The Evolutionary Trigger for Phytopathogenesis", "description": "Many publications highlight the importance of nitric oxide (NO) in plant-bacteria interactions, either in the promotion of health and plant growth or in pathogenesis. However, the role of NO in the signaling between bacteria and plants and in the fate of their interaction, as well as the reconstruction of their interactive evolution, remains largely unknown. Despite the complexity of the evolution of life on Earth, we explore the hypothesis that denitrification and aerobic respiration were responsible for local NO accumulation, which triggered primordial antagonistic biotic interactions, namely the first phytopathogenic interactions. N-oxides, including NO, could globally accumulate via lightning synthesis in the early anoxic ocean and constitute pools for the evolution of denitrification, considered an early step of the biological nitrogen cycle. Interestingly, a common evolution may be proposed for components of denitrification and aerobic respiration pathways, namely for NO and oxygen reductases, a theory compatible with the presence of low amounts of oxygen before the great oxygenation event (GOE), which was generated by Cyanobacteria. During GOE, the increase in oxygen caused the decrease of Earth's temperature and the consequent increase of oxygen dissolution and availability, making aerobic respiration an increasingly dominant trait of the expanding mesophilic lifestyle. Horizontal gene transfer was certainly important in the joint expansion of mesophily and aerobic respiration. First denitrification steps lead to NO formation through nitrite reductase activity, and NO may further accumulate when oxygen binds NO reductase, resulting in denitrification blockage. The consequent transient NO surplus in an oxic niche could have been a key factor for a successful outcome of an early denitrifying prokaryote able to scavenge oxygen by NO/oxygen reductase or by an independent heterotrophic aerobic respiration pathway. In fact, NO surplus could result in toxicity causing 'the first disease' in oxygen-producing Cyanobacteria. We inspected in bacteria the presence of sequences similar to the NO-producing nitrite reductase nirS gene of Thermus thermophilus, an extreme thermophilic aerobe of the Thermus/Deinococcus group, which constitutes an ancient lineage related to Cyanobacteria. In silico analysis revealed the relationship between the presence of nirS genes and phytopathogenicity in Gram-negative bacteria.", "keywords": ["aerobic respiration", "0301 basic medicine", "denitrification", "Thermus thermophilus", "nitrite reductase NirS", "Horizontal gene transfer", "Denitrific", "Microbiology", "QR1-502", "Nitrite reductase NirS", "Ationerobic respiration", "03 medical and health sciences", "13. Climate action", "horizontal gene transfer"]}, "links": [{"href": "https://repositorio.ulisboa.pt/bitstream/10451/51022/1/Santana%20et%20al%202017.pdf"}, {"href": "https://doi.org/10451/51022"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10451/51022", "name": "item", "description": "10451/51022", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10451/51022"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-10-10T00:00:00Z"}}, {"id": "10261/356392", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:21:38Z", "type": "Journal Article", "created": "2021-12-19", "title": "Stimulation of ammonia oxidizer and denitrifier abundances by nitrogen loading: Poor predictability for increased soil N2O emission", "description": "Abstract

Unprecedented nitrogen (N) inputs into terrestrial ecosystems have profoundly altered soil N cycling. Ammonia oxidizers and denitrifiers are the main producers of nitrous oxide (N2O), but it remains unclear how ammonia oxidizer and denitrifier abundances will respond to N loading and whether their responses can predict N\uffe2\uff80\uff90induced changes in soil N2O emission. By synthesizing 101 field studies worldwide, we showed that N loading significantly increased ammonia oxidizer abundance by 107% and denitrifier abundance by 45%. The increases in both ammonia oxidizer and denitrifier abundances were primarily explained by N loading form, and more specifically, organic N loading had stronger effects on their abundances than mineral N loading. Nitrogen loading increased soil N2O emission by 261%, whereas there was no clear relationship between changes in soil N2O emission and shifts in ammonia oxidizer and denitrifier abundances. Our field\uffe2\uff80\uff90based results challenge the laboratory\uffe2\uff80\uff90based hypothesis that increased ammonia oxidizer and denitrifier abundances by N loading would directly cause higher soil N2O emission. Instead, key abiotic factors (mean annual precipitation, soil pH, soil C:N ratio, and ecosystem type) explained N\uffe2\uff80\uff90induced changes in soil N2O emission. Altogether, these findings highlight the need for considering the roles of key abiotic factors in regulating soil N transformations under N loading to better understand the microbially mediated soil N2O emission.Free water surface (FWS) wetlands can be used to treat agricultural runoff, thereby reducing diffuse pollution. However, as these are highly dynamic systems, their design is still challenging. Complex models tend to require detailed information for calibration, which can only be obtained when the wetland is constructed. Hence simplified models are widely used for FWS wetlands design. The limitations of these models in full-scale FWS wetlands is that these systems often cope with stochastic events with different input concentrations. In our study, we compared different simple transport and degradation models for total nitrogen under steady- and unsteady-state conditions using information collected from a tracer experiment and data from two precipitation events from a full-scale FWS wetland. The tanks-in-series model proved to be robust for simulating solute transport, and the first-order degradation model with non-zero background concentration performed best for total nitrogen concentrations. However, the optimal background concentration changed from event to event. Thus, to use the model as a design tool, it is advisable to include an upper and lower background concentration to determine a range of wetland performance under different events. Models under steady- and unsteady-state conditions with simulated data showed good performance, demonstrating their potential for wetland design.

Denitrification is a permanent nitrogen removal pathway; thus, it is a desirable ecosystem function in water bodies receiving agricultural runoff. Knowledge of denitrification capacity in response to vegetation type and varying NO3\u2212 loads is essential for designing effectively constructed wetlands to control eutrophication. The aim of this study was to compare the nitrogen removal efficiency of two common wetland macrophytes, i.e., Phragmites australis and Typha latifolia in a NO3\u2212 enrichment experiment (50\u2212800 \u00b5M). Measurements of NO3\u2212 consumption, and N2 production were performed in vegetated and unvegetated mesocosms incubated in summer (26 \u00b0C) at biomass peak. Vegetated sediments demonstrated higher efficiency in converting NO3\u2212 to N2 via denitrification (<600\u201318,000 \u00b5mol N m\u22122 h\u22121) than bare sediments (300\u20133300 \u00b5mol N m\u22122 h\u22121). However, the denitrification stimulation effect from NO3\u2212 pulsing differed significantly between plant types. It can be hypothesized that P. australis played a more beneficial role than T. latifolia due to its greater submerged surface area, which facilitated enhanced opportunities for contact between NO3\u2212 and denitrifying bacteria. This ultimately resulted in an increased treatment performance. Understanding the interactions between plants and environmental drivers regulating denitrification is critical information for optimal wetland species selection. With an increasing global focus on sustainable water quality management, this research provides valuable insights into optimizing nature-based solutions.

", "keywords": ["biofilms; constructed wetlands; denitrification; nature-based solutions; nitrate pollution; P. australis; T. latifolia"]}, "links": [{"href": "https://sfera.unife.it/bitstream/11392/2582471/1/water-17-00560-v2.pdf"}, {"href": "https://www.mdpi.com/2073-4441/17/4/560/pdf"}, {"href": "https://doi.org/11392/2582471"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11392/2582471", "name": "item", "description": "11392/2582471", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11392/2582471"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-02-14T00:00:00Z"}}, {"id": "20.500.12556/RUL-155883", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:22:12Z", "type": "Journal Article", "created": "2024-04-01", "title": "Mineral and organic fertilisation influence ammonia oxidisers and denitrifiers and nitrous oxide emissions in a long-term tillage experiment", "description": "Nitrous oxide (N2O) emissions from different agricultural systems have been studied extensively to understand the mechanisms underlying their formation. While a number of long-term field experiments have focused on individual agricultural practices in relation to N2O emissions, studies on the combined effects of multiple practices are lacking. This study evaluated the effect of different tillage [no-till (NT) vs. conventional plough tillage (CT)] in combination with fertilisation [mineral (MIN), compost (ORG), and unfertilised control (CON)] on seasonal N2O emissions and the underlying N-cycling microbial community in one maize growing season. Rainfall events after fertilisation, which resulted in increased soil water content, were the main triggers of the observed N2O emission peaks. The highest cumulative emissions were measured in MIN fertilisation, followed by ORG and CON fertilisation. In the period after the first fertilisation CT resulted in higher cumulative emissions than NT, while no significant effect of tillage was observed cumulatively across the entire season. A higher genetic potential for N2O emissions was observed under NT than CT, as indicated by an increased (nirK\u00a0+\u00a0nirS)/(nosZI\u00a0+\u00a0nosZII) ratio. The mentioned ratio under NT decreased in the order CON > MIN\u00a0>\u00a0ORG, indicating a higher N2O consumption potential in the NT-ORG treatment, which was confirmed in terms of cumulative emissions. The AOB/16S ratio was strongly affected by fertilisation and was higher in the MIN than in the ORG and CON treatments, regardless of the tillage system. Multiple regression has revealed that this ratio is one of the most important variables explaining cumulative N2O emissions, possibly reflecting the role of bacterial ammonia oxidisers in minerally fertilised soil. Although the AOB/16S ratio aligned well with the measured N2O emissions in our experimental field, the higher genetic potential for denitrification expressed by the (nirK\u00a0+\u00a0nirS)/(nosZI\u00a0+\u00a0nosZII) ratio in NT than CT was not realized in the form of increased emissions. Our results suggest that organic fertilisation in combination with NT shows a promising combination for mitigating N2O emissions; however, addressing the yield gap is necessary before incorporating it in recommendations for farmers.", "keywords": ["du\u0161ik", "2. Zero hunger", "compost", "denitrification", "denitrifikacija", "N$_2$O", "kompost", "15. Life on land", "sonaravno kmetijstvo", "nitrifikacija", "nitrification", "6. Clean water", "conservation agriculture", "N-cycle", "13. Climate action", "ohranitveno kmetijstvo", "info:eu-repo/classification/udc/631.4"]}, "links": [{"href": "https://doi.org/20.500.12556/RUL-155883"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "20.500.12556/RUL-155883", "name": "item", "description": "20.500.12556/RUL-155883", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/20.500.12556/RUL-155883"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-06-01T00:00:00Z"}}, {"id": "2268/262547", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:22:21Z", "type": "Journal Article", "created": "2021-03-18", "title": "Dynamics of nitrous oxide with depth in groundwater: Insights from ambient groundwater and laboratory incubation experiments (Hesbaye chalk aquifer, Belgium)", "description": "Aquifers under agricultural areas are considered to be an indirect source of nitrous oxide emission (N2O) to the atmosphere, which is the greenhouse gas (GHGs) characterized with the highest global warning potential and acts as a stratospheric ozone depletion agent. Previous investigations performed in the Cretaceous Hesbaye chalk aquifer in Eastern Belgium suggested that the dynamics of N2O in the aquifer is controlled by overlapping biochemical processes such as nitrification and denitrification. The current study aims to obtain better insight concerning the factors controlling the distribution of N2O concentration along a vertical dimension in the aquifer, and to capture and quantify the occurrence of nitrification and denitrification processes in the groundwater system. Low-flow groundwater sampling technique was undertaken at different depths in the aquifer to collect groundwater samples aiming at obtaining information about ambient aquifer hydrogeochemical conditions and their effect on the accumulation of GHGs. Afterwards, laboratory stable isotope experiments, using NO3- and NH4+ compounds labeled with heavy 15N isotope, were applied to quantify the rates of nitrification and denitrification processes. Ambient studies suggest that the occurrence of N transformation was related to denitrification while laboratory incubation experiments did not detect it. Such controversial results might be explained by the discrepancy between real aquifer conditions and lab design studies. Thus, additional in situ tracer experiments should be carried out in areas where natural groundwater fluxes do not flush the injected tracer too rapidly. In addition, it would be useful to conduct microbiological studies to obtain better insight into the nature of subsurface biofilm biotope.", "keywords": ["Sciences aquatiques & oc\u00e9anologie", "Physique", " chimie", " math\u00e9matiques & sciences de la terre", "Nitrous Oxide", "01 natural sciences", "Low-flow sampling", "Ing\u00e9nierie", " informatique & technologie", "Calcium Carbonate", "Physical", " chemical", " mathematical & earth Sciences", "Belgium", "Groundwater", "0105 earth and related environmental sciences", "2. Zero hunger", "Aquatic sciences & oceanology", "N stable isotope analysis", "Life sciences", "Nitrification", "Geological", " petroleum & mining engineering", "6. Clean water", "Engineering", " computing & technology", "Sciences de la terre & g\u00e9ographie physique", "Greenhouse gases", "13. Climate action", "Earth sciences & physical geography", "Sciences du vivant", "Denitrification", "G\u00e9ologie", " ing\u00e9nierie du p\u00e9trole & des mines", "Laboratories"]}, "links": [{"href": "https://orbi.uliege.be/bitstream/2268/262547/2/Dynamics%20of%20nitrous%20oxide%20with%20depth%20in%20groundwater%20insights%20from%20ambient%20groundwater.pdf"}, {"href": "https://doi.org/2268/262547"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Contaminant%20Hydrology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2268/262547", "name": "item", "description": "2268/262547", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2268/262547"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-08-01T00:00:00Z"}}, {"id": "3138664797", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:22:45Z", "type": "Journal Article", "created": "2021-03-18", "title": "Dynamics of nitrous oxide with depth in groundwater: Insights from ambient groundwater and laboratory incubation experiments (Hesbaye chalk aquifer, Belgium)", "description": "Aquifers under agricultural areas are considered to be an indirect source of nitrous oxide emission (N2O) to the atmosphere, which is the greenhouse gas (GHGs) characterized with the highest global warning potential and acts as a stratospheric ozone depletion agent. Previous investigations performed in the Cretaceous Hesbaye chalk aquifer in Eastern Belgium suggested that the dynamics of N2O in the aquifer is controlled by overlapping biochemical processes such as nitrification and denitrification. The current study aims to obtain better insight concerning the factors controlling the distribution of N2O concentration along a vertical dimension in the aquifer, and to capture and quantify the occurrence of nitrification and denitrification processes in the groundwater system. Low-flow groundwater sampling technique was undertaken at different depths in the aquifer to collect groundwater samples aiming at obtaining information about ambient aquifer hydrogeochemical conditions and their effect on the accumulation of GHGs. Afterwards, laboratory stable isotope experiments, using NO3- and NH4+ compounds labeled with heavy 15N isotope, were applied to quantify the rates of nitrification and denitrification processes. Ambient studies suggest that the occurrence of N transformation was related to denitrification while laboratory incubation experiments did not detect it. Such controversial results might be explained by the discrepancy between real aquifer conditions and lab design studies. Thus, additional in situ tracer experiments should be carried out in areas where natural groundwater fluxes do not flush the injected tracer too rapidly. In addition, it would be useful to conduct microbiological studies to obtain better insight into the nature of subsurface biofilm biotope.", "keywords": ["2. Zero hunger", "Nitrous Oxide", "N stable isotope analysis", "Nitrification", "01 natural sciences", "Low-flow sampling", "6. Clean water", "Calcium Carbonate", "Greenhouse gases", "Belgium", "13. Climate action", "Denitrification", "Laboratories", "Groundwater", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/3138664797"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Contaminant%20Hydrology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "3138664797", "name": "item", "description": "3138664797", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/3138664797"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-08-01T00:00:00Z"}}, {"id": "35944518", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:22:55Z", "type": "Journal Article", "created": "2022-08-09", "title": "N\u2010damo, an opportunity to reduce methane emissions?", "keywords": ["Highlight", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "15. Life on land", "7. Clean energy", "6. Clean water", "12. Responsible consumption", "03 medical and health sciences", "13. Climate action", "11. Sustainability", "Denitrification", "Anaerobiosis", "Methane", "Oxidation-Reduction", "Nitrites"], "contacts": [{"organization": "G\u00f3mez\u2010Gallego, Tamara", "roles": ["creator"]}]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1758-2229.13114"}, {"href": "https://doi.org/35944518"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "35944518", "name": "item", "description": "35944518", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/35944518"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-08-09T00:00:00Z"}}, {"id": "PMC9152356", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-23T16:24:27Z", "type": "Journal Article", "created": "2022-05-17", "title": "Interacting Bioenergetic and Stoichiometric Controls on Microbial Growth", "description": "

Microorganisms function as open systems that exchange matter and energy with their surrounding environment. Even though mass (carbon and nutrients) and energy exchanges are tightly linked, there is a lack of integrated approaches that combine these fluxes and explore how they jointly impact microbial growth. Such links are essential to predicting how the growth rate of microorganisms varies, especially when the stoichiometry of carbon- (C) and nitrogen (N)-uptake is not balanced. Here, we present a theoretical framework to quantify the microbial growth rate for conditions of C-, N-, and energy-(co-) limitations. We use this framework to show how the C:N ratio and the degree of reduction of the organic matter (OM), which is also the electron donor, availability of electron acceptors (EAs), and the different sources of N together control the microbial growth rate under C, nutrient, and energy-limited conditions. We show that the growth rate peaks at intermediate values of the degree of reduction of OM under oxic and C-limited conditions, but not under N-limited conditions. Under oxic conditions and with N-poor OM, the growth rate is higher when the inorganic N (NInorg)-source is ammonium compared to nitrate due to the additional energetic cost involved in nitrate reduction. Under anoxic conditions, when nitrate is both EA and NInorg-source, the growth rates of denitrifiers and microbes performing the dissimilatory nitrate reduction to ammonia (DNRA) are determined by both OM degree of reduction and nitrate-availability. Consistent with the data, DNRA is predicted to foster growth under extreme nitrate-limitation and with a reduced OM, whereas denitrifiers are favored as nitrate becomes more available and in the presence of oxidized OM. Furthermore, the growth rate is reduced when catabolism is coupled to low energy yielding EAs (e.g., sulfate) because of the low carbon use efficiency (CUE). However, the low CUE also decreases the nutrient demand for growth, thereby reducing N-limitation. We conclude that bioenergetics provides a useful conceptual framework for explaining growth rates under different metabolisms and multiple resource-limitations.Unprecedented nitrogen (N) inputs into terrestrial ecosystems have profoundly altered soil N cycling. Ammonia oxidizers and denitrifiers are the main producers of nitrous oxide (N2O), but it remains unclear how ammonia oxidizer and denitrifier abundances will respond to N loading and whether their responses can predict N\uffe2\uff80\uff90induced changes in soil N2O emission. By synthesizing 101 field studies worldwide, we showed that N loading significantly increased ammonia oxidizer abundance by 107% and denitrifier abundance by 45%. The increases in both ammonia oxidizer and denitrifier abundances were primarily explained by N loading form, and more specifically, organic N loading had stronger effects on their abundances than mineral N loading. Nitrogen loading increased soil N2O emission by 261%, whereas there was no clear relationship between changes in soil N2O emission and shifts in ammonia oxidizer and denitrifier abundances. Our field\uffe2\uff80\uff90based results challenge the laboratory\uffe2\uff80\uff90based hypothesis that increased ammonia oxidizer and denitrifier abundances by N loading would directly cause higher soil N2O emission. Instead, key abiotic factors (mean annual precipitation, soil pH, soil C:N ratio, and ecosystem type) explained N\uffe2\uff80\uff90induced changes in soil N2O emission. Altogether, these findings highlight the need for considering the roles of key abiotic factors in regulating soil N transformations under N loading to better understand the microbially mediated soil N2O emission.