{"type": "FeatureCollection", "features": [{"id": "10.1007/s10021-020-00497-5", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-21T16:14:37Z", "type": "Journal Article", "created": "2020-03-30", "title": "Biocrusts Modulate Responses of Nitrous Oxide and Methane Soil Fluxes to Simulated Climate Change in a Mediterranean Dryland", "description": "Little is known about the role of biocrusts in regulating the responses of N2O and CH4 fluxes to climate change in drylands. Here, we aim to help filling this knowledge gap by using an 8-year field experiment in central Spain where temperature and rainfall are being manipulated (~\u20091.9\u00b0C warming, 33% rainfall reduction and their combination) in areas with and without well-developed biocrust communities. Areas with initial high cover of well-developed biocrusts showed lower N2O emissions, enhanced CH4 uptake and higher abundances of functional genes linked to N2O and CH4 fluxes compared with areas with poorly developed biocrusts. Moreover, biocrusts modulated the responses of gases emissions and related functional genes to warming and rainfall reductions. Specifically, we found under rainfall exclusion and its combination with warming a sharp reduction in N2O fluxes (~\u200996% and ~\u2009197%, respectively) only under well-developed biocrust cover. Warming and its combination with rainfall exclusion reduced CH4 consumption in areas with initial low cover of well-developed biocrust, whereas rainfall exclusion enhanced CH4 uptake only in areas with high initial cover of well-developed biocrusts. Similarly, the combination of warming and rainfall exclusion increased the abundance of the nosZ gene compared to the rainfall exclusion treatment and increased the abundance of the pmoA gene compared to the control, but only in areas with low biocrust cover. Taken together, our results indicate that well-developed biocrust communities could counteract the impact of warming and altered rainfall patterns on soil N2O and CH4 fluxes, highlighting their importance and the need to preserve them to minimize climate change impacts on drylands. A. L. is supported by a FPI fellowship from the Spanish Ministry of Economy and Competitiveness (BES-2014-067831). M.D-B. acknowledges support from the Marie Sklodowska-Curie Actions of the Horizon 2020 Framework Programme H2020-MSCA-IF-2016 under REA Grant Agreement No. 702057 (CLIMIFUN) and the BES Grant Agreement No. LRA17 1193 (MUSGONET). J.D acknowledges support from the Funda\u00e7\u00e3o para Ci\u00eancia e Tecnologia (IF/00950/2014) and the FEDER, within the PT2020 Partnership Agreement and COMPETE 2020 (UID/BIA/04004/2013). This research was supported by the European Research Council (ERC Grant Agreements 242658 [BIOCOM] and 647038 [BIODESERT]), by the Spanish Ministry of Economy and Competitiveness (BIOMOD project, ref. CGL2013-44661-R and AGL2015-64582-C3-3-R project) and by the Comunidad de Madrid and European Structural and Investment Funds (AGRISOST-CM S2013/ABI-2717). F.T.M. acknowledges support from Generalitat Valenciana (BIOMORES project, ref. CIDEGENT/2018/041). B.K.S research on the topic of biodiversity and ecosystem functions is funded by Australian Research Council (DP170104634).", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "arid regions", "Nitrous oxide", "nitrous oxide", "Mediterranean Region", "methane", "Ecolog\u00eda", "15. Life on land", "climatic changes", "Dryland", "03 medical and health sciences", "Methanotrophs", "13. Climate action", "XXXXXX - Unknown", "Biocrust", "crust vegetation", "Denitrifiers", "denitrifying bacteria", "Methane"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s10021-020-00497-5.pdf"}, {"href": "https://doi.org/10.1007/s10021-020-00497-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecosystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10021-020-00497-5", "name": "item", "description": "10.1007/s10021-020-00497-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10021-020-00497-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-30T00:00:00Z"}}, {"id": "10.1111/geb.12928", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-21T16:17:37Z", "type": "Journal Article", "created": "2019-05-14", "title": "Global drivers of methane oxidation and denitrifying gene distribution in drylands", "description": "AbstractAim<p>Microorganisms carrying pmoA and nosZ genes are major drivers of methane and nitrous oxide fluxes from soils. However, most studies on these organisms have been conducted in mesic ecosystems; therefore, little is known about the factors driving their distribution in drylands, the largest biome on Earth. We conducted a global survey to evaluate the role of climate\uffe2\uff80\uff90 and soil\uffe2\uff80\uff90related variables as predictors of the richness, abundance and community structure of bacteria carrying pmoA and nosZ genes.</p>Location<p>Eighty dryland ecosystems distributed worldwide.</p>Time period<p>From February 2006 to December 2011.</p>Major taxa studied<p>Methanotrophic (carrying the pmoA gene) and denitrifiying (carrying the nosZ gene) bacteria.</p>Methods<p>We used data from a field survey and structural equation modelling to evaluate the direct and indirect effects of climatic (aridity, rainfall seasonality and mean annual temperature) and soil (organic carbon, pH and texture) variables on the total abundance, richness and community structure of microorganisms carrying pmoA and nosZ genes.</p>Results<p>Taxa related to Methylococcus capsulatus or Methylocapsa sp., often associated with mesic environments, were common in global drylands. The abundance and richness of methanotrophs were not associated with climate or soil properties. However, mean annual temperature, rainfall seasonality, organic C, pH and sand content were highly correlated with their community structure. Aridity and soil variables, such as sand content and pH, were correlated with the abundance, community structure and richness of the nosZ bacterial community.</p>Main conclusions<p>Our study provides new insights into the drivers of the abundance, richness and community structure of soil microorganisms carrying pmoA and nosZ genes in drylands worldwide. We highlight how ongoing climate change will alter the structure of soil microorganisms, which might affect the net CH4 exchange and will probably reduce the capacity of dryland soils to carry out the final step of denitrification, favouring net N2O emissions.</p", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "arid regions", "550", "oxidation", "methane", "15. Life on land", "03 medical and health sciences", "methanotrophs", "abundance", " richness", " community structure", " drylands", " methanotrophs", " denitrifiers", "13. Climate action", "abundance", " community structure", " denitrifiers", " drylands", " methanotrophs", " richness", "denitrifying bacteria"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/geb.12928"}, {"href": "https://doi.org/10.1111/geb.12928"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Ecology%20and%20Biogeography", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/geb.12928", "name": "item", "description": "10.1111/geb.12928", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/geb.12928"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-13T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2016.07.023", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-09-21T16:16:16Z", "type": "Journal Article", "created": "2016-08-03", "title": "Response Of Microbial Functional Groups Involved In Soil N Cycle To N, P And Np Fertilization In Tibetan Alpine Meadows", "description": "The nitrogen (N) cycle is an important part of earth's biogeochemical cycles and N is a critical element for all life. Whereas the response to N - and more rarely phosphorus, P - fertilization of some microbial groups involved in soil N cycling has been studied, a comprehensive view of how the major microbial groups involved in soil N dynamics respond to combined N and P fertilization is lacking, which restricts our understanding of ecosystem responses to fertilization. Here we investigated the effects of different N, P and NP fertilizer levels (4 N levels without P; 4 P levels without N; and 4 P levels with constant N addition) on the abundances of 9 microbial groups involved in N dynamics. Real time PCR was used to target free N2 fixers, nitrifiers (bacterial and archaea ammonia oxidizers, AOB and AOA, respectively; and the nitrite oxidizers Nitrobacter and Nitrospira), nitrate reducers, nirK- and nirS-nitrite reducers, and nitrous oxide reducers. Soil physical-chemical characteristics and potential nitrification, PNR, were also measured. N fertilization increased the abundances of AOB and Nitrobacter but did not affect the abundances of the other groups. P fertilization decreased the abundances of N2 fixers, nitrate reducers and AOA, and increased the abundances of Nitrobacter and nitrous oxide reducers. NP fertilization decreased the abundances of AOA and nirK-nitrite reducers. Using a correlation network analysis, we demonstrate the strong coupling generally observed in these grasslands between N2 fixers, AOA, Nitrospira, narG-nitrate reducers and nirK-denitrifiers (most of them responding to N/P availability, and being known to be favored by low oxygen availability); and between AOB and Nitrobacter (known to be favored by high oxygen and high N levels) that controlled changes in PNR. The observed (de)coupling between the responses of the different microbial groups may have major consequences for N cycling and N losses from fertilized Tibetan alpine meadows.", "keywords": ["580", "2. Zero hunger", "0301 basic medicine", "Nitrogen", "[SDV]Life Sciences [q-bio]", "Phosphorus", "04 agricultural and veterinary sciences", "Nitrifiers", "15. Life on land", "6. Clean water", "3. Good health", "[SDV] Life Sciences [q-bio]", "03 medical and health sciences", "Grassland soil", "N-2 fixers", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Denitrifiers"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2016.07.023"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2016.07.023", "name": "item", "description": "10.1016/j.soilbio.2016.07.023", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2016.07.023"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-10-01T00:00:00Z"}}, {"id": "10.5281/zenodo.3572794", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-21T16:21:07Z", "type": "Journal Article", "created": "2019-05-14", "title": "Global drivers of methane oxidation and denitrifying gene distribution in drylands", "description": "AbstractAim<p>Microorganisms carrying pmoA and nosZ genes are major drivers of methane and nitrous oxide fluxes from soils. However, most studies on these organisms have been conducted in mesic ecosystems; therefore, little is known about the factors driving their distribution in drylands, the largest biome on Earth. We conducted a global survey to evaluate the role of climate\uffe2\uff80\uff90 and soil\uffe2\uff80\uff90related variables as predictors of the richness, abundance and community structure of bacteria carrying pmoA and nosZ genes.</p>Location<p>Eighty dryland ecosystems distributed worldwide.</p>Time period<p>From February 2006 to December 2011.</p>Major taxa studied<p>Methanotrophic (carrying the pmoA gene) and denitrifiying (carrying the nosZ gene) bacteria.</p>Methods<p>We used data from a field survey and structural equation modelling to evaluate the direct and indirect effects of climatic (aridity, rainfall seasonality and mean annual temperature) and soil (organic carbon, pH and texture) variables on the total abundance, richness and community structure of microorganisms carrying pmoA and nosZ genes.</p>Results<p>Taxa related to Methylococcus capsulatus or Methylocapsa sp., often associated with mesic environments, were common in global drylands. The abundance and richness of methanotrophs were not associated with climate or soil properties. However, mean annual temperature, rainfall seasonality, organic C, pH and sand content were highly correlated with their community structure. Aridity and soil variables, such as sand content and pH, were correlated with the abundance, community structure and richness of the nosZ bacterial community.</p>Main conclusions<p>Our study provides new insights into the drivers of the abundance, richness and community structure of soil microorganisms carrying pmoA and nosZ genes in drylands worldwide. We highlight how ongoing climate change will alter the structure of soil microorganisms, which might affect the net CH4 exchange and will probably reduce the capacity of dryland soils to carry out the final step of denitrification, favouring net N2O emissions.</p", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "methanotrophs", "arid regions", "550", "oxidation", "13. Climate action", "methane", "abundance", " community structure", " denitrifiers", " drylands", " methanotrophs", " richness", "15. Life on land", "denitrifying bacteria"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/geb.12928"}, {"href": "https://doi.org/10.5281/zenodo.3572794"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Ecology%20and%20Biogeography", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.3572794", "name": "item", "description": "10.5281/zenodo.3572794", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.3572794"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-13T00:00:00Z"}}, {"id": "1959.7/uws:56895", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-21T16:22:29Z", "type": "Journal Article", "created": "2020-03-30", "title": "Biocrusts Modulate Responses of Nitrous Oxide and Methane Soil Fluxes to Simulated Climate Change in a Mediterranean Dryland", "description": "Little is known about the role of biocrusts in regulating the responses of N2O and CH4 fluxes to climate change in drylands. Here, we aim to help filling this knowledge gap by using an 8-year field experiment in central Spain where temperature and rainfall are being manipulated (~\u20091.9\u00b0C warming, 33% rainfall reduction and their combination) in areas with and without well-developed biocrust communities. Areas with initial high cover of well-developed biocrusts showed lower N2O emissions, enhanced CH4 uptake and higher abundances of functional genes linked to N2O and CH4 fluxes compared with areas with poorly developed biocrusts. Moreover, biocrusts modulated the responses of gases emissions and related functional genes to warming and rainfall reductions. Specifically, we found under rainfall exclusion and its combination with warming a sharp reduction in N2O fluxes (~\u200996% and ~\u2009197%, respectively) only under well-developed biocrust cover. Warming and its combination with rainfall exclusion reduced CH4 consumption in areas with initial low cover of well-developed biocrust, whereas rainfall exclusion enhanced CH4 uptake only in areas with high initial cover of well-developed biocrusts. Similarly, the combination of warming and rainfall exclusion increased the abundance of the nosZ gene compared to the rainfall exclusion treatment and increased the abundance of the pmoA gene compared to the control, but only in areas with low biocrust cover. Taken together, our results indicate that well-developed biocrust communities could counteract the impact of warming and altered rainfall patterns on soil N2O and CH4 fluxes, highlighting their importance and the need to preserve them to minimize climate change impacts on drylands. A. L. is supported by a FPI fellowship from the Spanish Ministry of Economy and Competitiveness (BES-2014-067831). M.D-B. acknowledges support from the Marie Sklodowska-Curie Actions of the Horizon 2020 Framework Programme H2020-MSCA-IF-2016 under REA Grant Agreement No. 702057 (CLIMIFUN) and the BES Grant Agreement No. LRA17 1193 (MUSGONET). J.D acknowledges support from the Funda\u00e7\u00e3o para Ci\u00eancia e Tecnologia (IF/00950/2014) and the FEDER, within the PT2020 Partnership Agreement and COMPETE 2020 (UID/BIA/04004/2013). This research was supported by the European Research Council (ERC Grant Agreements 242658 [BIOCOM] and 647038 [BIODESERT]), by the Spanish Ministry of Economy and Competitiveness (BIOMOD project, ref. CGL2013-44661-R and AGL2015-64582-C3-3-R project) and by the Comunidad de Madrid and European Structural and Investment Funds (AGRISOST-CM S2013/ABI-2717). F.T.M. acknowledges support from Generalitat Valenciana (BIOMORES project, ref. CIDEGENT/2018/041). B.K.S research on the topic of biodiversity and ecosystem functions is funded by Australian Research Council (DP170104634).", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "arid regions", "Nitrous oxide", "nitrous oxide", "Mediterranean Region", "methane", "Ecolog\u00eda", "15. Life on land", "climatic changes", "Dryland", "03 medical and health sciences", "Methanotrophs", "13. Climate action", "XXXXXX - Unknown", "Biocrust", "crust vegetation", "Denitrifiers", "denitrifying bacteria", "Methane"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s10021-020-00497-5.pdf"}, {"href": "https://doi.org/1959.7/uws:56895"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecosystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "1959.7/uws:56895", "name": "item", "description": "1959.7/uws:56895", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/1959.7/uws:56895"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-30T00:00:00Z"}}, {"id": "2946732851", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-21T16:22:57Z", "type": "Journal Article", "created": "2019-05-14", "title": "Global drivers of methane oxidation and denitrifying gene distribution in drylands", "description": "AbstractAim<p>Microorganisms carrying pmoA and nosZ genes are major drivers of methane and nitrous oxide fluxes from soils. However, most studies on these organisms have been conducted in mesic ecosystems; therefore, little is known about the factors driving their distribution in drylands, the largest biome on Earth. We conducted a global survey to evaluate the role of climate\uffe2\uff80\uff90 and soil\uffe2\uff80\uff90related variables as predictors of the richness, abundance and community structure of bacteria carrying pmoA and nosZ genes.</p>Location<p>Eighty dryland ecosystems distributed worldwide.</p>Time period<p>From February 2006 to December 2011.</p>Major taxa studied<p>Methanotrophic (carrying the pmoA gene) and denitrifiying (carrying the nosZ gene) bacteria.</p>Methods<p>We used data from a field survey and structural equation modelling to evaluate the direct and indirect effects of climatic (aridity, rainfall seasonality and mean annual temperature) and soil (organic carbon, pH and texture) variables on the total abundance, richness and community structure of microorganisms carrying pmoA and nosZ genes.</p>Results<p>Taxa related to Methylococcus capsulatus or Methylocapsa sp., often associated with mesic environments, were common in global drylands. The abundance and richness of methanotrophs were not associated with climate or soil properties. However, mean annual temperature, rainfall seasonality, organic C, pH and sand content were highly correlated with their community structure. Aridity and soil variables, such as sand content and pH, were correlated with the abundance, community structure and richness of the nosZ bacterial community.</p>Main conclusions<p>Our study provides new insights into the drivers of the abundance, richness and community structure of soil microorganisms carrying pmoA and nosZ genes in drylands worldwide. We highlight how ongoing climate change will alter the structure of soil microorganisms, which might affect the net CH4 exchange and will probably reduce the capacity of dryland soils to carry out the final step of denitrification, favouring net N2O emissions.</p", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "arid regions", "550", "oxidation", "methane", "15. Life on land", "03 medical and health sciences", "methanotrophs", "abundance", " richness", " community structure", " drylands", " methanotrophs", " denitrifiers", "13. Climate action", "abundance", " community structure", " denitrifiers", " drylands", " methanotrophs", " richness", "denitrifying bacteria"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/geb.12928"}, {"href": "https://doi.org/2946732851"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Ecology%20and%20Biogeography", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2946732851", "name": "item", "description": "2946732851", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2946732851"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-13T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Denitrifiers&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Denitrifiers&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Denitrifiers&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Denitrifiers&offset=6", "hreflang": "en-US"}], "numberMatched": 6, "numberReturned": 6, "distributedFeatures": [], "timeStamp": "2026-09-21T16:45:46.169747Z"}