The interplay between metal contamination and climate change may exacerbate the negative impact on the soil microbiome and, consequently, on soil health and ecosystem services. We assessed the response of the microbial community of a heavy metal-contaminated soil when exposed to short-term (48 h) variations in air temperature, soil humidity or ultraviolet (UV) radiation in the absence and presence of Enchytraeus crypticus (soil invertebrate). Each of the climate scenarios simulated significantly altered at least one of the microbial parameters measured. Irrespective of the presence or absence of invertebrates, the effects were particularly marked upon exposure to increased air temperature and alterations in soil moisture levels (drought and flood scenarios). The observed effects can be partly explained by significant alterations in soil properties such as pH, dissolved organic carbon, and water-extractable heavy metals, which were observed for all scenarios in comparison to standard conditions. The occurrence of invertebrates mitigated some of the impacts observed on the soil microbial community, particularly in bacterial abundance, richness, diversity, and metabolic activity. Our findings emphasize the importance of considering the interplay between climate change, anthropogenic pressures, and soil biotic components to assess the impact of climate change on terrestrial ecosystems and to develop and implement effective management strategies.
", "keywords": ["0301 basic medicine", "2. Zero hunger", "Soil invertebrates", "Soil microbiome", "Soil drought", "Ultraviolet Rays", "Soil pollution", "Microbiota", "Temperature", "Enchytraeus crypticus", "15. Life on land", "01 natural sciences", "Article", "6. Clean water", "Soil", "03 medical and health sciences", "13. Climate action", "UVR exposure", "Metals", " Heavy", "Climate change", "Soil flood", "Metagenomics", "Increased temperature", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.3390/genes15010107"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/genes15010107", "name": "item", "description": "10.3390/genes15010107", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/genes15010107"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-16T00:00:00Z"}}, {"id": "10259/7490", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:25:28Z", "type": "Journal Article", "created": "2022-10-19", "title": "Comparative toxicological assessment of three soils polluted with different levels of hydrocarbons and heavy metals using in vitro and in vivo approaches", "description": "The biological effects induced by the pollutants present in soils, together with the chemical and physical characterizations, are good indicators to provide a general overview of their quality. However, the existence of studies where the toxicity associated to soils contaminated with mixtures of pollutants applying both in vitro and in vivo models are scarce. In this work, three soils (namely, Soil 001, Soil 002 and Soil 013) polluted with different concentrations of hydrocarbons and heavy metals were evaluated using different organisms representative of human (HepG2 human cell line) and environmental exposure (the yeast Saccharomyces cerevisiae, the Gram-negative bacterium Pseudomonas putida and, for the in vivo evaluation, the annelid Enchytraeus crypticus). In vitro assays showed that the soluble fraction of the Soil 001, which presented the highest levels of heavy metals, represented a great impact in the viability of the HepG2 cells and S. cerevisiae, while organic extracts from Soils 002 and 013 caused a slight decrease in the viability of HepG2 cells. In addition, in vivo experiments showed that Soils 001 and 013 affected the survival and the reproduction of E. crypticus. Altogether, these results provide a general overview of the potential hazards associated to three specific contaminated sites in a variety of organisms, showing how different concentrations of similar pollutants affect them, and highlights the relevance of testing both organic and soluble extracts when in vitro safety assays of soils are performed.", "keywords": ["Pseudomonas putida", "Enchytraeus crypticus", "Qu\u00edmica anal\u00edtica", "Saccharomyces cerevisiae", "Analytic", "01 natural sciences", "Hydrocarbons", "6. Clean water", "Chemistry", "Soil", "Soil contamination", "13. Climate action", "Metals", " Heavy", "Humans", "Soil Pollutants", "Chemistry", " Analytic", "HepG2 cells Saccharomyces cerevisiae Pseudomonas putida Enchytraeus crypticus Soil contamination", "HepG2 cells", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10259/7490"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10259/7490", "name": "item", "description": "10259/7490", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10259/7490"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-01T00:00:00Z"}}, {"id": "10400.14/44005", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:25:38Z", "type": "Journal Article", "created": "2024-01-16", "title": "Structural and Functional Shifts in the Microbial Community of a Heavy Metal-Contaminated Soil Exposed to Short-Term Changes in Air Temperature, Soil Moisture and UV Radiation", "description": "The interplay between metal contamination and climate change may exacerbate the negative impact on the soil microbiome and, consequently, on soil health and ecosystem services. We assessed the response of the microbial community of a heavy metal-contaminated soil when exposed to short-term (48 h) variations in air temperature, soil humidity or ultraviolet (UV) radiation in the absence and presence of Enchytraeus crypticus (soil invertebrate). Each of the climate scenarios simulated significantly altered at least one of the microbial parameters measured. Irrespective of the presence or absence of invertebrates, the effects were particularly marked upon exposure to increased air temperature and alterations in soil moisture levels (drought and flood scenarios). The observed effects can be partly explained by significant alterations in soil properties such as pH, dissolved organic carbon, and water-extractable heavy metals, which were observed for all scenarios in comparison to standard conditions. The occurrence of invertebrates mitigated some of the impacts observed on the soil microbial community, particularly in bacterial abundance, richness, diversity, and metabolic activity. Our findings emphasize the importance of considering the interplay between climate change, anthropogenic pressures, and soil biotic components to assess the impact of climate change on terrestrial ecosystems and to develop and implement effective management strategies.
", "keywords": ["2. Zero hunger", "0301 basic medicine", "Soil invertebrates", "Soil microbiome", "Soil drought", "Ultraviolet Rays", "Soil pollution", "Microbiota", "Temperature", "Enchytraeus crypticus", "15. Life on land", "01 natural sciences", "Article", "6. Clean water", "Soil", "03 medical and health sciences", "13. Climate action", "UVR exposure", "Metals", " Heavy", "Climate change", "Soil flood", "Metagenomics", "Increased temperature", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10400.14/44005"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10400.14/44005", "name": "item", "description": "10400.14/44005", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10400.14/44005"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-16T00:00:00Z"}}, {"id": "10773/25425", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:25:45Z", "type": "Journal Article", "created": "2019-02-01", "title": "Soil moisture influences the avoidance behavior of invertebrate species in anthropogenic metal(loid)-contaminated soils", "description": "Water availability is paramount in the response of soil invertebrates towards stress situations. This study aimed to evaluate the effects of forecasted soil moisture scenarios on the avoidance behavior of two invertebrate species (the arthropod Folsomia candida and the soft-bodied oligochaete Enchytraeus crypticus) in soils degraded by different types of anthropogenic metal(loid) contamination (mining soil and agricultural soil affected by industrial chemical wastes). Different soil moisture contents (expressed as % of the soil water holding capacity, WHC) were evaluated: 50% (standard soil moisture conditions for soil invertebrates' tests); 75% (to simulate increasing soil water availability after intense rainfalls and/or floods); 40%, 30%, 25% and 20% (to simulate decreasing soil water availability during droughts). Invertebrates' avoidance behavior and changes in soil porewater major ions and metal(loid)s were assessed after 48\u202fh exposure. Soil incubations induced a general solubilization/mobilization of porewater major ions, while higher soil acidity favored the solubilization/mobilization of porewater metal(loid)s, especially at 75% WHC. Folsomia candida preferred soils moistened at 50% WHC, regardless the soils were contaminated or not and the changing soil porewater characteristics. Enchytraeus crypticus avoided metal(loid) contamination, but this depended on the soil moisture conditions and the corresponding changes in porewater characteristics: enchytraeids lost their capacity to avoid contaminated soils under water stress situations (75% and 20-25% WHC), but also when contaminated soils had greater water availability than control soils. Therefore, forecasted soil moisture scenarios induced by global warming changed soil porewater composition and invertebrates capacity to avoid metal(loid)-contaminated soils.", "keywords": ["2. Zero hunger", "Global warming", "Water", "Metal(loid) availability", "Enchytraeus crypticus", "Agriculture", "15. Life on land", "Global Warming", "01 natural sciences", "Mining", "6. Clean water", "Folsomia candida", "Soil", "Metals", "13. Climate action", "Avoidance Learning", "Animals", "Soil Pollutants", "Oligochaeta", "Multiple stressors", "Environmental Pollution", "Arthropods", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10773/25425"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10773/25425", "name": "item", "description": "10773/25425", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10773/25425"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Enchytraeus+crypticus&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Enchytraeus+crypticus&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Enchytraeus+crypticus&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Enchytraeus+crypticus&offset=7", "hreflang": "en-US"}], "numberMatched": 7, "numberReturned": 7, "distributedFeatures": [], "timeStamp": "2026-06-25T09:22:36.718171Z"}