{"type": "FeatureCollection", "features": [{"id": "10.1002/jsfa.4349", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:02Z", "type": "Journal Article", "created": "2011-03-29", "title": "Efficiency Of Urease And Nitrification Inhibitors In Reducing Ammonia Volatilization From Diverse Nitrogen Fertilizers Applied To Different Soil Types And Wheat Straw Mulching", "description": "Some authors suggest that the absence of tillage in agricultural soils might have an influence on the efficiency of nitrogen applied in the soil surface. In this study we investigate the influence of no-tillage and soil characteristics on the efficiency of a urease inhibitor (N-(n-butyl)thiophosphoric triamide, NBPT) and a nitrification inhibitor (diciandiamide, DCD) in decreasing ammonia volatilization from urea and ammonium nitrate (AN), respectively.The results indicate that ammonia volatilization in soils amended with urea was significantly higher than in those fertilized with AN. Likewise, the main soil factors affecting ammonia volatilization from urea are clay and sand soil contents. While clay impedes ammonia volatilization, sand favours it. The presence of organic residues on soil surface (no-tillage) tends to increase ammonia volatilization from urea, although this fact depended on soil type. The presence of NBPT in urea fertilizer significantly reduced soil ammonia volatilization. This action of NBPT was negatively affected by acid soil pH and favoured by soil clay content.The presence of organic residues on soil surface amended with urea increased ammonia volatilization, and was particularly high in sandy compared with clay soils. Application of NBPT reduced ammonia volatilization although its efficiency is reduced in acid soils. Concerning AN fertilization, there were no differences in ammonia volatilization with or without DCD in no-tillage soils.", "keywords": ["2. Zero hunger", "Nitrates", "Plant Stems", "Nitrogen", "Agriculture", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "Silicon Dioxide", "Nitrification", "Urease", "6. Clean water", "Soil", "Organophosphorus Compounds", "Ammonia", "Clay", "Urea", "0401 agriculture", " forestry", " and fisheries", "Aluminum Silicates", "Enzyme Inhibitors", "Volatilization", "Fertilizers", "Humic Substances", "Triticum"]}, "links": [{"href": "https://doi.org/10.1002/jsfa.4349"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.4349", "name": "item", "description": "10.1002/jsfa.4349", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.4349"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-03-29T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2012.04.043", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:15:36Z", "type": "Journal Article", "created": "2012-05-13", "title": "Effectiveness Of Urease Inhibition On The Abatement Of Ammonia, Nitrous Oxide And Nitric Oxide Emissions In A Non-Irrigated Mediterranean Barley Field", "description": "Urea is considered the cheapest and most commonly used form of inorganic N fertilizer worldwide. However, its use is associated with emissions of ammonia (NH(3)), nitrous oxide (N(2)O) and nitric oxide (NO), which have both economic and environmental impact. Urease activity inhibitors have been proposed as a means to reduce NH(3) emissions, although limited information exists about their effect on N(2)O and NO emissions. In this context, a field experiment was carried out with a barley crop (Hordeum vulgare L.) under Mediterranean conditions to test the effectiveness of the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) on reducing these gaseous N losses from surface applied urea. Crop yield, soil mineral N concentrations, dissolved organic carbon (DOC), denitrification potential, NH(3), N(2)O and NO fluxes were measured during the growing season. The inclusion of the inhibitor reduced NH(3) emissions in the 30 d following urea application by 58% and net N(2)O and NO emissions in the 95 d following urea application by 86% and 88%, respectively. NBPT addition also increased grain yield by 5% and N uptake by 6%, although neither increase was statistically significant. Under the experimental conditions presented here, these results demonstrate the potential of the urease inhibitor NBPT in abating NH(3), N(2)O and NO emissions from arable soils fertilized with urea, slowing urea hydrolysis and releasing lower concentrations of NH(4)(+) to the upper soil layer.", "keywords": ["2. Zero hunger", "Nitrous Oxide", "Hordeum", "04 agricultural and veterinary sciences", "Nitric Oxide", "Urease", "6. Clean water", "Organophosphorus Compounds", "Ammonia", "Air Pollution", "Urea", "0401 agriculture", " forestry", " and fisheries", "Enzyme Inhibitors", "Fertilizers"]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2012.04.043"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2012.04.043", "name": "item", "description": "10.1016/j.chemosphere.2012.04.043", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2012.04.043"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-09-01T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2020.123208", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:16:22Z", "type": "Journal Article", "created": "2020-06-17", "title": "Effects of prescription antibiotics on soil- and root-associated microbiomes and resistomes in an agricultural context", "description": "The use of treated wastewater for crop irrigation is rapidly increasing to respond to the ever-growing demands for water and food resources. However, this practice may contribute to the spread of antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARGs) in agricultural settings. To evaluate this potential risk, we analyzed microbiomes and resistomes of soil and Lactuca sativa L. (lettuce) root samples from pots irrigated with tap water spiked with 0, 20, or 100 \u03bcg L-1 of a mixture of three antibiotics (Trimethoprim, Ofloxacin, Sulfamethoxazole). The presence of antibiotics induced changes in bacterial populations, particularly in soil, as revealed by 16S rDNA sequence analysis. Parallel shotgun sequencing identified a total of 56 different ARGs conferring resistance against 14 antibiotic families. Antibiotic -treated samples showed increased loads of ARGs implicated in mutidrug resistance or in both direct and indirect acquired resistance. These changes correlated with the prevalence of Xantomonadales species in the root microbiomes. We interpret these data as indicating different strategies of soil and root microbiomes to cope with the presence of antibiotics, and as a warning that their presence may increase the loads of ARBs and ARGs in edible plant parts, therefore constituting a potential risk for human consumers.", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Microbiota", "Angiotensin-Converting Enzyme Inhibitors", "Drug Resistance", " Microbial", "6. Clean water", "Anti-Bacterial Agents", "Angiotensin Receptor Antagonists", "Soil", "03 medical and health sciences", "Prescriptions", "Genes", " Bacterial", "Humans", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.jhazmat.2020.123208"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2020.123208", "name": "item", "description": "10.1016/j.jhazmat.2020.123208", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2020.123208"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-01T00:00:00Z"}}, {"id": "10.1016/j.watres.2021.116818", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:17:06Z", "type": "Journal Article", "created": "2021-01-08", "title": "Antibiotic resistance gene load and irrigation intensity determine the impact of wastewater irrigation on antimicrobial resistance in the soil microbiome", "description": "Treated wastewater (TWW) irrigation is a useful counter-measure against the depletion of freshwater (FW) resources. However, TWW contains several contaminants of emerging concern, such as antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARGs). Thus, TWW irrigation might promote the spread of antimicrobial resistance in soil environments. In the present work, we hypothesized that the ARG load and irrigation intensity define the effect of TWW irrigation on ARG spread dynamics in soil. This hypothesis was tested using a multiphase approach: a) comparing soil from a full-scale, commercially operated, TWW irrigated field with non-irrigated soil, b) long-term sampling of the TWW irrigated field over one year with different irrigation intensities and intercepted by irrigation breaks and c) laboratory-scale soil microcosms irrigated with TWW compared to FW. Six ARGs, the integrase gene intI1 and the 16S rRNA were quantified using qPCR. In addition, effects of TWW irrigation on bacterial community composition of microcosm-samples were analysed with 16S rRNA amplicon sequencing. The genes sul1, qnrS, blaOXA-58, tet(M) and intI1 were significantly more abundant in the TWW irrigated field soil, whereas blaCTX--M-32 and blaTEM, the least abundant genes in the TWW irrigation, showed higher abundance in the non-irrigated soil. The relative abundance of sul1, qnrS, blaOXA-58, tet(M) and intI1 correlated with TWW irrigation intensity and decreased during irrigation breaks. Despite the decrease, the levels of these genes remained consistently higher than the non-irrigated soil indicating persistence upon their introduction into the soil. Microcosm experiments verified observations from the field study: TWW irrigation promoted the spread of ARGs and intI1 into soil at far elevated levels compared to FW irrigation. However, the impact of TWW irrigation on 16S rRNA absolute abundance and the soil microbial community composition was negligible. In conclusion, the impact of TWW irrigation depends mainly on the introduced ARG load and the irrigation intensity.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Agricultural Irrigation", "Microbiota", "Angiotensin-Converting Enzyme Inhibitors", "Wastewater", "15. Life on land", "01 natural sciences", "6. Clean water", "Anti-Bacterial Agents", "Angiotensin Receptor Antagonists", "Soil", "03 medical and health sciences", "Genes", " Bacterial", "RNA", " Ribosomal", " 16S", "Drug Resistance", " Bacterial", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.watres.2021.116818"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2021.116818", "name": "item", "description": "10.1016/j.watres.2021.116818", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2021.116818"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-04-01T00:00:00Z"}}, {"id": "10.1021/acs.est.1c00612", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:17:11Z", "type": "Journal Article", "created": "2021-04-28", "title": "Hidden Resistome: Enrichment Reveals the Presence of Clinically Relevant Antibiotic Resistance Determinants in Treated Wastewater-Irrigated Soils", "description": "Treated-wastewater (TW) irrigation transfers antibiotic-resistant bacteria (ARB) to soil, but persistence of these bacteria is generally low due to resilience of the soil microbiome. Nonetheless, wastewater-derived bacteria and associated antibiotic resistance genes (ARGs) may persist below detection levels and potentially proliferate under copiotrophic conditions. To test this hypothesis, we exposed soils from microcosm, lysimeter, and field experiments to short-term enrichment in copiotroph-stimulating media. In microcosms, enrichment stimulated growth of multidrug-resistant Escherichia coli up to 2 weeks after falling below detection limits. Lysimeter and orchard soils irrigated in-tandem with either freshwater or TW were subjected to culture-based, qPCR and shotgun metagenomic analyses prior, and subsequent, to enrichment. Although native TW- and freshwater-irrigated soil microbiomes and resistomes were similar to each other, enrichment resulted in higher abundances of cephalosporin- and carbapenem-resistant Enterobacteriaceae and in substantial differences in the composition of microbial communities and ARGs. Enrichment stimulated ARG-harboring Bacillaceae in the freshwater-irrigated soils, whereas in TWW-irrigated soils, ARG-harboring \u03b3-proteobacterial families Enterobacteriaceae and Moraxellaceae were more profuse. We demonstrate that TW-derived ARB and associated ARGs can persist at below detection levels in irrigated soils and believe that similar short-term enrichment strategies can be applied for environmental antimicrobial risk assessment in the future.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Agricultural Irrigation", "Angiotensin-Converting Enzyme Inhibitors", "Drug Resistance", " Microbial", "Wastewater", "6. Clean water", "Anti-Bacterial Agents", "3. Good health", "Angiotensin Receptor Antagonists", "Soil", "03 medical and health sciences", "Genes", " Bacterial", "Humans", "Soil Microbiology"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.1c00612"}, {"href": "https://doi.org/10.1021/acs.est.1c00612"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.est.1c00612", "name": "item", "description": "10.1021/acs.est.1c00612", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.est.1c00612"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-04-27T00:00:00Z"}}, {"id": "10.1093/femsec/fiaa058", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:12Z", "type": "Journal Article", "created": "2020-03-27", "title": "Persistence of wastewater antibiotic resistant bacteria and their genes in human fecal material", "description": "ABSTRACT<p>Domestic wastewater is a recognized source of antibiotic resistant bacteria and antibiotic resistance genes (ARB&amp;ARGs), whose risk of transmission to humans cannot be ignored. The fitness of wastewater ARB in the complex fecal microbiota of a healthy human was investigated in feces-based microcosm assays (FMAs). FMAs were inoculated with two wastewater isolates, Escherichia coli strain A2FCC14 (MLST ST131) and Enterococcus faecium strain H1EV10 (MLST ST78), harboring the ARGs blaTEM, blaCTX, blaOXA-A and vanA, respectively. The FMAs, incubated in the presence or absence of oxygen or in the presence or absence of the antibiotics cefotaxime or vancomycin, were monitored based on cultivation, ARGs quantification and bacterial community analysis. The fecal bacterial community was dominated by members of the phyla Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria and Verrucomicrobia. The ARGs harbored by the wastewater isolates could be quantified after one week, in FMAs incubated under both aerobic and anaerobic conditions. These observations were not significantly different in FMAs incubated anaerobically, supplemented with sub-inhibitory concentrations of cefotaxime or vancomycin. The observation that ARGs of wastewater ARB persisted in presence of the human fecal microbiota for at least one week supports the hypothesis of a potential transmission to humans, a topic that deserves further investigation.</p>", "keywords": ["0301 basic medicine", "Microcosm assays", "0303 health sciences", "Bacteria", "Angiotensin-Converting Enzyme Inhibitors", "Wastewater", "Human fecal microbiota", "6. Clean water", "Anti-Bacterial Agents", "3. Good health", "Angiotensin Receptor Antagonists", "Feces", "03 medical and health sciences", "Antibiotic resistance genes", "Antibiotic resistant bacteria", "Genes", " Bacterial", "11. Sustainability", "Humans", "Antibiotic resistance transmission", "Microcosm effect", "Multilocus Sequence Typing"]}, "links": [{"href": "http://academic.oup.com/femsec/article-pdf/96/6/fiaa058/33327470/fiaa058.pdf"}, {"href": "https://doi.org/10.1093/femsec/fiaa058"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/femsec/fiaa058", "name": "item", "description": "10.1093/femsec/fiaa058", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/femsec/fiaa058"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-02T00:00:00Z"}}, {"id": "11828476", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:25:02Z", "type": "Journal Article", "created": "2002-08-25", "title": "The Outstanding Biological Stability of - and -Peptides toward Proteolytic Enzymes: An In Vitro Investigation with Fifteen Peptidases", "description": "A series of 36 linear and cyclic beta- and gamma-peptides consisting of as few as two, and as many as 15 residues, was offered as substrates to 15 commercially available proteases of bacterial, fungal, and eukaryotic origin, including a beta-lactamase and amidases, as well as most vigorous, nonspecific proteases, such as the 20S proteasome from human erythrocytes. For comparison, an alpha-eicosapeptide and standard substrates of the proteolytic enzymes were included in the investigation. Under conditions of complete cleavage of the alpha-peptide within 15 min the beta- and gamma-peptides were stable for at least 48 h. Inhibition studies with seven beta- and gamma-peptides and alpha-chymotrypsin show that the residual enzyme activity toward succinyl-Ala-Ala-Pro-Phe-p-nitroanilide is unchanged within experimental error after incubation for 15 min with the peptide analogues. Thus, beta- and gamma-peptides with proteinogenic side chains, that is, consisting of the singly or doubly homologated natural alpha-amino acids (one or two CH(2) groups inserted in the backbone of each residue), are completely stable to common proteases, without inhibiting their normal activity (as demonstrated for alpha-chymotrypsin). This proteolytic stability of peptides built of homologated amino acids is a prerequisite for their potential use as drugs.", "keywords": ["0301 basic medicine", "0303 health sciences", "Molecular Structure", "Peptides", " Cyclic", "Protein Structure", " Tertiary", "3. Good health", "Fungal Proteins", "03 medical and health sciences", "Bacterial Proteins", "Catalytic Domain", "Humans", "Enzyme Inhibitors", "Peptides", "Chromatography", " High Pressure Liquid", "Peptide Hydrolases", "Protein Binding"]}, "links": [{"href": "https://doi.org/11828476"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ChemBioChem", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11828476", "name": "item", "description": "11828476", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11828476"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2001-06-01T00:00:00Z"}}, {"id": "20864344", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:25:31Z", "type": "Journal Article", "created": "2010-09-08", "title": "Discovery and optimization of adamantyl carbamate inhibitors of 11\u03b2-HSD1", "description": "Synthesis of 2-adamantyl carbamate derivatives of piperidines and pyrrolidines led to the discovery of 9a with an IC(50) of 15.2 nM against human 11\u03b2-HSD1 in adipocytes. Optimization for increased adipocyte potency, metabolic stability and selectivity afforded 11k and 11l, both of which were >25% orally bioavailable in rat.", "keywords": ["Models", " Molecular", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "11-beta-Hydroxysteroid Dehydrogenase Type 1", "Drug Discovery", "Animals", "Adamantane", "Enzyme Inhibitors", "Rats", "3. Good health"]}, "links": [{"href": "https://doi.org/20864344"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioorganic%20%26amp%3B%20Medicinal%20Chemistry%20Letters", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "20864344", "name": "item", "description": "20864344", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/20864344"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-11-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Enzyme+Inhibitors&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Enzyme+Inhibitors&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Enzyme+Inhibitors&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Enzyme+Inhibitors&offset=8", "hreflang": "en-US"}], "numberMatched": 8, "numberReturned": 8, "distributedFeatures": [], "timeStamp": "2026-07-27T07:55:26.795635Z"}