{"type": "FeatureCollection", "features": [{"id": "10.1007/s00442-005-0249-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:24Z", "type": "Journal Article", "created": "2005-10-04", "title": "Fungal Community Composition And Metabolism Under Elevated Co2 And O-3", "description": "Atmospheric CO(2) and O(3) concentrations are increasing due to human activity and both trace gases have the potential to alter C cycling in forest ecosystems. Because soil microorganisms depend on plant litter as a source of energy for metabolism, changes in the amount or the biochemistry of plant litter produced under elevated CO(2) and O(3) could alter microbial community function and composition. Previously, we have observed that elevated CO(2) increased the microbial metabolism of cellulose and chitin, whereas elevated O(3) dampened this response. We hypothesized that this change in metabolism under CO(2) and O(3) enrichment would be accompanied by a concomitant change in fungal community composition. We tested our hypothesis at the free-air CO(2) and O(3) enrichment (FACE) experiment at Rhinelander, Wisconsin, in which Populus tremuloides, Betula papyrifera, and Acer saccharum were grown under factorial CO(2) and O(3) treatments. We employed extracellular enzyme analysis to assay microbial metabolism, phospholipid fatty acid (PLFA) analysis to determine changes in microbial community composition, and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to analyze the fungal community composition. The activities of 1,4-beta-glucosidase (+37%) and 1,4,-beta-N-acetylglucosaminidase (+84%) were significantly increased under elevated CO(2), whereas 1,4-beta-glucosidase activity (-25%) was significantly suppressed by elevated O(3). There was no significant main effect of elevated CO(2) or O(3) on fungal relative abundance, as measured by PLFA. We identified 39 fungal taxonomic units from soil using DGGE, and found that O(3) enrichment significantly altered fungal community composition. We conclude that fungal metabolism is altered under elevated CO(2) and O(3), and that there was a concomitant change in fungal community composition under elevated O(3). Thus, changes in plant inputs to soil under elevated CO(2) and O(3) can propagate through the microbial food web to alter the cycling of C in soil.", "keywords": ["0106 biological sciences", "Food Chain", "Extracellular Enzymes", "Science", "Ecology and Evolutionary Biology", "Polymerase Chain Reaction\u2013Denaturing Gradient Gel Electrophoresis", "Polymerase Chain Reaction", "01 natural sciences", "Soil Microbial Community", "Soil", "Ozone", "Health Sciences", "Acetylglucosaminidase", "Cellular and Developmental Biology", "Ecosystem", "Soil Microbiology", "beta-Glucosidase", "Fatty Acids", "Fungi", "Natural Resources and Environment", "Molecular", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Fungal Metabolism", "Carbon", "Free-air CO 2 and O 3 Enrichment", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Extracellular Space"]}, "links": [{"href": "https://doi.org/10.1007/s00442-005-0249-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-005-0249-3", "name": "item", "description": "10.1007/s00442-005-0249-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-005-0249-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-10-05T00:00:00Z"}}, {"id": "10.1016/j.agrformet.2025.110503", "type": "Feature", "geometry": null, "properties": {"license": "Restricted", "updated": "2026-07-26T16:15:19Z", "type": "Journal Article", "created": "2025-03-18", "title": "Linking soil extracellular enzymes with soil respiration under altered litter inputs", "description": "<p>Climate and land-use changes have altered both litter quality and quantity, with cascading impacts on soil respiration (SR). Soil extracellular enzymes (EEs) like cellulase and ligninase are crucial for deconstructing plant litter because they convert polymers into monomers. However, whether and how changes in litter inputs influence soil cellulase and ligninase activities as well as the implications for SR remain poorly understood. We conducted a global meta-analysis of 827 observations on the responses of SR and soil cellulase and ligninase activities to litter addition and litter removal. Litter addition significantly increased cellulase activity by 25 %, whereas litter removal decreased it by 26 %. Neither litter addition nor litter removal affected ligninase activity. Changes in cellulase activity correlated positively with SR under both litter addition and litter removal, but no such relationship was found for ligninase activity. These results indicate that changes in litter inputs affect SR primarily by affecting the microbial decomposition of readily decomposable rather than more structurally complex carbon pools. In addition, the effects of changes in litter inputs on cellulase activity decreased with treatment duration, suggesting that the long-term effects of changes in litter inputs on SR might be smaller than previously thought. Our results underscore the dominant role of cellulase in mediating the responses of SR to altered litter inputs. Integrating cellulase responses to altered litter inputs into Earth system models could improve the representation of microbial processes and refine the predictions of soil carbon dynamics.</p>", "keywords": ["Soil carbon pools", "Litter alternations", "Soil respiration", "Global changes", "Soil extracellular enzymes", "Soil microorganisms"]}, "links": [{"href": "https://doi.org/10.1016/j.agrformet.2025.110503"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agricultural%20and%20Forest%20Meteorology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agrformet.2025.110503", "name": "item", "description": "10.1016/j.agrformet.2025.110503", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agrformet.2025.110503"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-05-01T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2007.01313.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:42Z", "type": "Journal Article", "created": "2007-01-19", "title": "Plant Species Richness, Elevated Co2, And Atmospheric Nitrogen Deposition Alter Soil Microbial Community Composition And Function", "description": "Abstract<p>We determined soil microbial community composition and function in a field experiment in which plant communities of increasing species richness were exposed to factorial elevated CO2 and nitrogen (N) deposition treatments. Because elevated CO2 and N deposition increased plant productivity to a greater extent in more diverse plant assemblages, it is plausible that heterotrophic microbial communities would experience greater substrate availability, potentially increasing microbial activity, and accelerating soil carbon (C) and N cycling. We, therefore, hypothesized that the response of microbial communities to elevated CO2 and N deposition is contingent on the species richness of plant communities. Microbial community composition was determined by phospholipid fatty acid analysis, and function was measured using the activity of key extracellular enzymes involved in litter decomposition. Higher plant species richness, as a main effect, fostered greater microbial biomass, cellulolytic and chitinolytic capacity, as well as the abundance of saprophytic and arbuscular mycorrhizal (AM) fungi. Moreover, the effect of plant species richness on microbial communities was significantly modified by elevated CO2 and N deposition. For instance, microbial biomass and fungal abundance increased with greater species richness, but only under combinations of elevated CO2 and ambient N, or ambient CO2 and N deposition. Cellobiohydrolase activity increased with higher plant species richness, and this trend was amplified by elevated CO2. In most cases, the effect of plant species richness remained significant even after accounting for the influence of plant biomass. Taken together, our results demonstrate that plant species richness can directly regulate microbial activity and community composition, and that plant species richness is a significant determinant of microbial response to elevated CO2 and N deposition. The strong positive effect of plant species richness on cellulolytic capacity and microbial biomass indicate that the rates of soil C cycling may decline with decreasing plant species richness.</p>", "keywords": ["Extracellular Enzymes", "Complementary Resource Use", "Science", "Ecology and Evolutionary Biology", "Grassland Ecosystem", "Phospholipid Fatty Acid (PLFA)", "Global Change", "14. Life underwater", "complimentary resource use", "global change", "580", "2. Zero hunger", "Plant Diversity", "microbial biomass", "Geology and Earth Sciences", "grasslands", "Soil Fungi", "extracellular enzymes", "04 agricultural and veterinary sciences", "15. Life on land", "Microbial Biomass", "Soil C Cycling", "plant diversity", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "FACE (Free-air Carbon Dioxide Enrichment)"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2486.2007.01313.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2007.01313.x", "name": "item", "description": "10.1111/j.1365-2486.2007.01313.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2007.01313.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-01-19T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2014.07.027", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:16:47Z", "type": "Journal Article", "created": "2014-08-24", "title": "Crop Rotation Complexity Regulates The Decomposition Of High And Low Quality Residues", "description": "While many ecosystem processes depend on biodiversity, the relationships between agricultural plant diversity and soil carbon (C) and nitrogen (N) dynamics remains controversial. Our objective was to examine how temporal plant diversity (i.e. crop rotation) influences residue decomposition, a key ecosystem function that regulates nutrient cycling, greenhouse gas emissions, and soil organic matter formation. We incubated soils from five long-term crop rotations, located at W.K. Kellogg Biological Station LTER in southwestern Michigan, USA, with and without four chemically diverse crop residues. Increasing crop biodiversity increased soil potentially mineralizable C by 125%, increased hydrolytic enzyme activity by 46%, but decreased oxidative enzyme activity by 20% in soils before residue was added. After residue additions, soils from more diverse cropping systems decomposed all residues more rapidly (0.2e8.3% greater mass loss) compared to monoculture corn. The fast-cycling, \u2018Active C\u2019 pool and microbial biomass N increased with higher cropping diversity, but the differences among rotations in Active C pools was higher for the most recalcitrant residues. Further, the ratio of the cellulose degrading enzyme ( b-glucosidase) to the lignin degrading enzyme (phenol oxidase) was highest in the two most diverse crop rotations regardless of residue additions, providing additional evidence of enhanced microbial activity and substrate acquisition in more diverse rotations. Our study shows that crop diversity over time influences the processing of newly-added residues, microbial dynamics, and nutrient cycling. Diversifying crop rotations has the potential to enhance soil ecosystem functions and is critical to maintaining soil services in agricultural systems.", "keywords": ["2. Zero hunger", "Nitrogen mineralization", "Litter quality", "Carbon mineralization", "Microbial biomass", "04 agricultural and veterinary sciences", "Extracellular enzymes", "Biogeochemistry", "15. Life on land", "01 natural sciences", "13. Climate action", "Plant biodiversity", "0401 agriculture", " forestry", " and fisheries", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2014.07.027"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2014.07.027", "name": "item", "description": "10.1016/j.soilbio.2014.07.027", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2014.07.027"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-11-01T00:00:00Z"}}, {"id": "10.1111/gcb.12996", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:18:33Z", "type": "Journal Article", "created": "2015-06-05", "title": "Microbial Physiology And Soil Co2 Efflux After 9 Years Of Soil Warming In A Temperate Forest - No Indications For Thermal Adaptations", "description": "Abstract<p>Thermal adaptations of soil microorganisms could mitigate or facilitate global warming effects on soil organic matter (SOM) decomposition and soil CO2 efflux. We incubated soil from warmed and control subplots of a forest soil warming experiment to assess whether 9\uffc2\uffa0years of soil warming affected the rates and the temperature sensitivity of the soil CO2 efflux, extracellular enzyme activities, microbial efficiency, and gross N mineralization. Mineral soil (0\uffe2\uff80\uff9310\uffc2\uffa0cm depth) was incubated at temperatures ranging from 3 to 23\uffc2\uffa0\uffc2\uffb0C. No adaptations to long\uffe2\uff80\uff90term warming were observed regarding the heterotrophic soil CO2 efflux (R10 warmed: 2.31\uffc2\uffa0\uffc2\uffb1\uffc2\uffa00.15\uffc2\uffa0\uffce\uffbcmol\uffc2\uffa0m\uffe2\uff88\uff922\uffc2\uffa0s\uffe2\uff88\uff921, control: 2.34\uffc2\uffa0\uffc2\uffb1\uffc2\uffa00.29\uffc2\uffa0\uffce\uffbcmol\uffc2\uffa0m\uffe2\uff88\uff922\uffc2\uffa0s\uffe2\uff88\uff921; Q10 warmed: 2.45\uffc2\uffa0\uffc2\uffb1\uffc2\uffa00.06, control: 2.45\uffc2\uffa0\uffc2\uffb1\uffc2\uffa00.04). Potential enzyme activities increased with incubation temperature, but the temperature sensitivity of the enzymes did not differ between the warmed and the control soils. The ratio of C\uffc2\uffa0:\uffc2\uffa0N acquiring enzyme activities was significantly higher in the warmed soil. Microbial biomass\uffe2\uff80\uff90specific respiration rates increased with incubation temperature, but the rates and the temperature sensitivity (Q10 warmed: 2.54\uffc2\uffa0\uffc2\uffb1\uffc2\uffa00.23, control 2.75\uffc2\uffa0\uffc2\uffb1\uffc2\uffa00.17) did not differ between warmed and control soils. Microbial substrate use efficiency (SUE) declined with increasing incubation temperature in both, warmed and control, soils. SUE and its temperature sensitivity (Q10 warmed: 0.84\uffc2\uffa0\uffc2\uffb1\uffc2\uffa00.03, control: 0.88\uffc2\uffa0\uffc2\uffb1\uffc2\uffa00.01) did not differ between warmed and control soils either. Gross N mineralization was invariant to incubation temperature and was not affected by long\uffe2\uff80\uff90term soil warming. Our results indicate that thermal adaptations of the microbial decomposer community are unlikely to occur in C\uffe2\uff80\uff90rich calcareous temperate forest soils.</p>", "keywords": ["0106 biological sciences", "570", "substrate use efficiency", "Nitrogen", "ARCTIC SOIL", "Acclimatization", "Forests", "soil CO2 efflux", "Global Warming", "01 natural sciences", "630", "COMMUNITY COMPOSITION", "BOREAL FOREST", "Soil", "gross N mineralization", "SEASONAL PATTERNS", "thermal adaptation", "EXTRACELLULAR ENZYMES", "CARBON-USE EFFICIENCY", "soil warming", "Enzyme activities", "BEECH FOREST", "ENZYME-ACTIVITY", "Soil Microbiology", "2. Zero hunger", "106022 Mikrobiologie", "Soil CO efflux", "NITROGEN AVAILABILITY", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Primary Research Articles", "Thermal adaptation", "enzyme activities", "13. Climate action", "Austria", "106022 Microbiology", "Soil warming", "0401 agriculture", " forestry", " and fisheries", "CYCLE FEEDBACKS", "Gross N mineralization", "Seasons", "Substrate use efficiency"]}, "links": [{"href": "https://doi.org/10.1111/gcb.12996"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.12996", "name": "item", "description": "10.1111/gcb.12996", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.12996"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-09-28T00:00:00Z"}}, {"id": "10.14288/1.0441432", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:19:23Z", "type": "Journal Article", "created": "2024-03-15", "title": "Crop Conversion from Annual to Perennials: An Effective Strategy to Affect Soil Multifunctionality", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Although crop conversion from annual to perennial crops has been considered as one path towards climate-smart and resource-efficient agriculture, the effects of this conversion on soil multifunctionality and biomass yields remain unclear. The objective of the study is to enhance soil multifunctionality while exerting a marginal influence on farmer income. Here, we investigated the effects of annual winter wheat (Triticum aestivum L.) and two perennial crops (a grass (Lolium perenne L.), a legume (Medicago sativa L.), and their mixture) on soil multifunctionality and biomass yield on the Yellow River floodplain. Soil multifunctionality was assessed by the capacity of water regulation and the multifunctionality of carbon (C), nitrogen (N), and phosphorus (P) cycles. C cycle multifunctionality index is the average of \u03b2-xylosidase, \u03b2-cellobiosidase, and \u03b2-1, 4-glucosidase. N cycle multifunctionality index is the average of L-leucine aminopeptidase and \u03b2-1, 4-N-acetyl-glucosaminidase, and acid phosphatase represented (and dominated) P cycle functions. The results showed that perennial crops enhanced soil multifunctionality by 207% for L. perenne, 311% for M. sativa, and 438% for L. perenne + M. sativa, compared with annual winter wheat (T. aestivum). The effect of perennial crops on soil multifunctionality increased with infiltration rate, dissolved organic C, microbial biomass C, and extracellular enzymatic activities for both C and N acquisition. However, we observed that perennial crops had a lower biomass yield than annual crop. Therefore, the transition of agricultural landscapes to perennials needs to take into account the balance between environmental protection and food security, as well as environmental heterogeneity, to promote sustainable agricultural development.</p></article>", "keywords": ["land use change", "2. Zero hunger", "soil extracellular enzymes", "annual and perennial crops", "Yellow River floodplain", "S", "13. Climate action", "Agriculture", "soil functions", "15. Life on land", "crop type", "6. Clean water", "12. Responsible consumption"]}, "links": [{"href": "https://doi.org/10.14288/1.0441432"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agronomy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.14288/1.0441432", "name": "item", "description": "10.14288/1.0441432", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.14288/1.0441432"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-03-15T00:00:00Z"}}, {"id": "10.1890/14-0088.1", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:19:47Z", "type": "Journal Article", "created": "2014-07-18", "title": "Plant Diversity Effects On Soil Microbial Functions And Enzymes Are Stronger Than Warming In A Grassland Experiment", "description": "<p>Anthropogenic changes in biodiversity and atmospheric temperature significantly influence ecosystem processes. However, little is known about potential interactive effects of plant diversity and warming on essential ecosystem properties, such as soil microbial functions and element cycling. We studied the effects of orthogonal manipulations of plant diversity (one, four, and 16 species) and warming (ambient, +1.5\uffc2\uffb0C, and +3\uffc2\uffb0C) on soil microbial biomass, respiration, growth after nutrient additions, and activities of extracellular enzymes in 2011 and 2012 in the BAC (biodiversity and climate) perennial grassland experiment site at Cedar Creek, Minnesota, USA. Focal enzymes are involved in essential biogeochemical processes of the carbon, nitrogen, and phosphorus cycles. Soil microbial biomass and some enzyme activities involved in the C and N cycle increased significantly with increasing plant diversity in both years. In addition, 16\uffe2\uff80\uff90species mixtures buffered warming induced reductions in topsoil water content. We found no interactive effects of plant diversity and warming on soil microbial biomass and growth rates. However, the activity of several enzymes (1,4\uffe2\uff80\uff90\uffce\uffb2\uffe2\uff80\uff90glucosidase, 1,4\uffe2\uff80\uff90\uffce\uffb2\uffe2\uff80\uff90N\uffe2\uff80\uff90acetylglucosaminidase, phosphatase, peroxidase) depended on interactions between plant diversity and warming with elevated activities of enzymes involved in the C, N, and P cycles at both high plant diversity and high warming levels. Increasing plant diversity consistently decreased microbial biomass\uffe2\uff80\uff90specific enzyme activities and altered soil microbial growth responses to nutrient additions, indicating that plant diversity changed nutrient limitations and/or microbial community composition. In contrast to our expectations, higher plant diversity only buffered temperature effects on soil water content, but not on microbial functions. Temperature effects on some soil enzymes were greatest at high plant diversity. In total, our results suggest that the fundamental temperature ranges of soil microbial communities may be sufficiently broad to buffer their functioning against changes in temperature and that plant diversity may be a dominant control of soil microbial processes in a changing world.</p>", "keywords": ["aboveground-belowground interactions", "Hot Temperature", "warming", "Climate Change", "biodiversity-ecosystem functioning", "global warming", "soil microbial ecology", "Soil", "XXXXXX - Unknown", "Biomass", "global change", "Soil Microbiology", "2. Zero hunger", "microbial biomass", "grasslands", "extracellular enzymes", "Biodiversity", "04 agricultural and veterinary sciences", "Plants", "15. Life on land", "plant diversity", "Enzymes", "grassland ecosystem", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "ecosystems"]}, "links": [{"href": "https://doi.org/10.1890/14-0088.1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1890/14-0088.1", "name": "item", "description": "10.1890/14-0088.1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1890/14-0088.1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-01-01T00:00:00Z"}}, {"id": "10.3389/ffgc.2021.686945", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:20:32Z", "type": "Journal Article", "created": "2021-06-11", "title": "Modeling Microbial Adaptations to Nutrient Limitation During Litter Decomposition", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Microbial decomposers face large stoichiometric imbalances when feeding on nutrient-poor plant residues. To meet the challenges of nutrient limitation, microorganisms might: (i) allocate less carbon (C) to growth vs. respiration or excretion (i.e., flexible C-use efficiency, CUE), (ii) produce extracellular enzymes to target compounds that supply the most limiting element, (iii) modify their cellular composition according to the external nutrient availability, and (iv) preferentially retain nutrients at senescence. These four resource use modes can have different consequences on the litter C and nitrogen (N) dynamics\u2013modes that selectively remove C from the system can reduce C storage in soil, whereas modes that delay C mineralization and increase internal N recycling could promote storage of C and N. Since we do not know which modes are dominant in litter decomposers, we cannot predict the fate of C and N released from plant residues, in particular under conditions of microbial nutrient limitation. To address this question, we developed a process-based model of litter decomposition in which these four resource use modes were implemented. We then parameterized the model using \u223c80 litter decomposition datasets spanning a broad range of litter qualities. The calibrated model variants were able to capture most of the variability in litter C, N, and lignin fractions during decomposition regardless of which modes were included. This suggests that different modes can lead to similar litter decomposition trajectories (thanks to the multiple alternative resource acquisition pathways), and that identification of dominant modes is not possible using \u201cstandard\u201d litter decomposition data (an equifinality problem). Our results thus point to the need of exploring microbial adaptations to nutrient limitation with empirical estimates of microbial traits and to develop models flexible enough to consider a range of hypothesized microbial responses.</p></article>", "keywords": ["2. Zero hunger", "microbial model", "carbon use efficiency", "nitrogen limitation", "Forestry", "extracellular enzymes", "litter decomposition", "04 agricultural and veterinary sciences", "Biological Sciences", "SD1-669.5", "15. Life on land", "microbial stoichiometry", "C/N ratio", "C:N ratio", "12. Responsible consumption", "Environmental sciences", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Biologiska vetenskaper", "GE1-350"]}, "links": [{"href": "https://doi.org/10.3389/ffgc.2021.686945"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Forests%20and%20Global%20Change", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/ffgc.2021.686945", "name": "item", "description": "10.3389/ffgc.2021.686945", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/ffgc.2021.686945"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-11T00:00:00Z"}}, {"id": "10.3390/agronomy14030594", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:20:41Z", "type": "Journal Article", "created": "2024-03-15", "title": "Crop Conversion from Annual to Perennials: An Effective Strategy to Affect Soil Multifunctionality", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Although crop conversion from annual to perennial crops has been considered as one path towards climate-smart and resource-efficient agriculture, the effects of this conversion on soil multifunctionality and biomass yields remain unclear. The objective of the study is to enhance soil multifunctionality while exerting a marginal influence on farmer income. Here, we investigated the effects of annual winter wheat (Triticum aestivum L.) and two perennial crops (a grass (Lolium perenne L.), a legume (Medicago sativa L.), and their mixture) on soil multifunctionality and biomass yield on the Yellow River floodplain. Soil multifunctionality was assessed by the capacity of water regulation and the multifunctionality of carbon (C), nitrogen (N), and phosphorus (P) cycles. C cycle multifunctionality index is the average of \u03b2-xylosidase, \u03b2-cellobiosidase, and \u03b2-1, 4-glucosidase. N cycle multifunctionality index is the average of L-leucine aminopeptidase and \u03b2-1, 4-N-acetyl-glucosaminidase, and acid phosphatase represented (and dominated) P cycle functions. The results showed that perennial crops enhanced soil multifunctionality by 207% for L. perenne, 311% for M. sativa, and 438% for L. perenne + M. sativa, compared with annual winter wheat (T. aestivum). The effect of perennial crops on soil multifunctionality increased with infiltration rate, dissolved organic C, microbial biomass C, and extracellular enzymatic activities for both C and N acquisition. However, we observed that perennial crops had a lower biomass yield than annual crop. Therefore, the transition of agricultural landscapes to perennials needs to take into account the balance between environmental protection and food security, as well as environmental heterogeneity, to promote sustainable agricultural development.</p></article>", "keywords": ["land use change", "2. Zero hunger", "soil extracellular enzymes", "annual and perennial crops", "Yellow River floodplain", "S", "13. Climate action", "Agriculture", "soil functions", "15. Life on land", "crop type", "6. Clean water", "12. Responsible consumption"]}, "links": [{"href": "https://doi.org/10.3390/agronomy14030594"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agronomy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/agronomy14030594", "name": "item", "description": "10.3390/agronomy14030594", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/agronomy14030594"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-03-15T00:00:00Z"}}, {"id": "10.5061/dryad.ht4t3", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:21:28Z", "type": "Dataset", "title": "Data from: Host phenology and potential saprotrophism of ectomycorrhizal fungi in the boreal forest", "description": "Open AccessPhenology-induced changes in carbon assimilation by trees may affect  carbon stored in fine roots and as a consequence, alter carbon allocated  to ectomycorrhizal fungi. Two competing models exist to explain carbon  mobilization by ectomycorrhizal fungi. Under the \u2018saprotrophy model\u2019,  decreased allocation of carbon may induce saprotrophic behaviour in  ectomycorrhizal fungi, resulting in the decomposition of organic matter to  mobilize carbon. Alternatively, under the \u2018nutrient acquisition model\u2019,  decomposition may instead be driven by the acquisition of nutrients locked  within soil organic matter compounds, with carbon mobilization a secondary  process. We tested whether phenology-induced shifts in carbon reserves of  fine roots of aspen (Populus tremuloides) affect potential activity of  four carbon-compound degrading enzymes, \u03b2-glucuronidase, \u03b2-glucosidase,  N-acetylglucosaminidase and laccase, by ectomycorrhizal fungi.  Ectomycorrhizal roots from mature aspen were collected across eight stands  in north-eastern Alberta, Canada, and analysed during tree dormancy, leaf  flush, full leaf expansion and leaf abscission. We predicted potential  extracellular enzyme activity to be highest when root carbon reserves were  lowest, should host phenology induce saprotrophism. Further, we  anticipated enzyme activity to be mediated by invertase, a plant-derived  enzyme which makes carbon available to fungal symbionts in the  plant\u2013fungus interface. Root carbon reserves were positively correlated  with invertase, suggesting phenology may affect carbon allocation to  ectomycorrhizal fungi. However, of the four enzymes, host phenology had  the largest effect on \u03b2-glucuronidase, but activity of this enzyme was not  correlated with root carbon reserves or invertase. Low-biomass  ectomycorrhizas had greater potential laccase activity than high-biomass  ectomycorrhizas, highlighting discrete functional traits in fungi for  litter decomposition. Our results suggest that the decomposition of  organic matter may be driven by foraging by fungi for nutrients locked  within organic compounds rather than for mobilizing carbon. Furthermore,  the potential ability to degrade lignin was more common in low-biomass  ectomycorrhizas when compared to high-biomass ectomycorrhizas.", "keywords": ["2. Zero hunger", "ectomycorrhizal exploration type", "extracellular enzymes", "15. Life on land", "nonstructural carbohydrates", "invertase"], "contacts": [{"organization": "Hupperts, Stefan F., Pritsch, Karin, Landh\u00e4usser, Simon M., Karst, Justine,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.ht4t3"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.ht4t3", "name": "item", "description": "10.5061/dryad.ht4t3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.ht4t3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-06-06T00:00:00Z"}}, {"id": "3167308845", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:26:13Z", "type": "Journal Article", "created": "2021-06-11", "title": "Modeling Microbial Adaptations to Nutrient Limitation During Litter Decomposition", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Microbial decomposers face large stoichiometric imbalances when feeding on nutrient-poor plant residues. To meet the challenges of nutrient limitation, microorganisms might: (i) allocate less carbon (C) to growth vs. respiration or excretion (i.e., flexible C-use efficiency, CUE), (ii) produce extracellular enzymes to target compounds that supply the most limiting element, (iii) modify their cellular composition according to the external nutrient availability, and (iv) preferentially retain nutrients at senescence. These four resource use modes can have different consequences on the litter C and nitrogen (N) dynamics\u2013modes that selectively remove C from the system can reduce C storage in soil, whereas modes that delay C mineralization and increase internal N recycling could promote storage of C and N. Since we do not know which modes are dominant in litter decomposers, we cannot predict the fate of C and N released from plant residues, in particular under conditions of microbial nutrient limitation. To address this question, we developed a process-based model of litter decomposition in which these four resource use modes were implemented. We then parameterized the model using \u223c80 litter decomposition datasets spanning a broad range of litter qualities. The calibrated model variants were able to capture most of the variability in litter C, N, and lignin fractions during decomposition regardless of which modes were included. This suggests that different modes can lead to similar litter decomposition trajectories (thanks to the multiple alternative resource acquisition pathways), and that identification of dominant modes is not possible using \u201cstandard\u201d litter decomposition data (an equifinality problem). Our results thus point to the need of exploring microbial adaptations to nutrient limitation with empirical estimates of microbial traits and to develop models flexible enough to consider a range of hypothesized microbial responses.</p></article>", "keywords": ["2. Zero hunger", "microbial model", "carbon use efficiency", "nitrogen limitation", "Forestry", "extracellular enzymes", "litter decomposition", "04 agricultural and veterinary sciences", "Biological Sciences", "SD1-669.5", "15. Life on land", "microbial stoichiometry", "C/N ratio", "C:N ratio", "12. Responsible consumption", "Environmental sciences", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Biologiska vetenskaper", "GE1-350"]}, "links": [{"href": "https://doi.org/3167308845"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Forests%20and%20Global%20Change", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "3167308845", "name": "item", "description": "3167308845", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/3167308845"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-11T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Extracellular+Enzymes&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Extracellular+Enzymes&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Extracellular+Enzymes&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Extracellular+Enzymes&offset=11", "hreflang": "en-US"}], "numberMatched": 11, "numberReturned": 11, "distributedFeatures": [], "timeStamp": "2026-07-26T17:57:10.102775Z"}