{"type": "FeatureCollection", "features": [{"id": "10.1007/s00442-005-0249-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:14:25Z", "type": "Journal Article", "created": "2005-10-04", "title": "Fungal Community Composition And Metabolism Under Elevated Co2 And O-3", "description": "Atmospheric CO(2) and O(3) concentrations are increasing due to human activity and both trace gases have the potential to alter C cycling in forest ecosystems. Because soil microorganisms depend on plant litter as a source of energy for metabolism, changes in the amount or the biochemistry of plant litter produced under elevated CO(2) and O(3) could alter microbial community function and composition. Previously, we have observed that elevated CO(2) increased the microbial metabolism of cellulose and chitin, whereas elevated O(3) dampened this response. We hypothesized that this change in metabolism under CO(2) and O(3) enrichment would be accompanied by a concomitant change in fungal community composition. We tested our hypothesis at the free-air CO(2) and O(3) enrichment (FACE) experiment at Rhinelander, Wisconsin, in which Populus tremuloides, Betula papyrifera, and Acer saccharum were grown under factorial CO(2) and O(3) treatments. We employed extracellular enzyme analysis to assay microbial metabolism, phospholipid fatty acid (PLFA) analysis to determine changes in microbial community composition, and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to analyze the fungal community composition. The activities of 1,4-beta-glucosidase (+37%) and 1,4,-beta-N-acetylglucosaminidase (+84%) were significantly increased under elevated CO(2), whereas 1,4-beta-glucosidase activity (-25%) was significantly suppressed by elevated O(3). There was no significant main effect of elevated CO(2) or O(3) on fungal relative abundance, as measured by PLFA. We identified 39 fungal taxonomic units from soil using DGGE, and found that O(3) enrichment significantly altered fungal community composition. We conclude that fungal metabolism is altered under elevated CO(2) and O(3), and that there was a concomitant change in fungal community composition under elevated O(3). Thus, changes in plant inputs to soil under elevated CO(2) and O(3) can propagate through the microbial food web to alter the cycling of C in soil.", "keywords": ["0106 biological sciences", "Food Chain", "Extracellular Enzymes", "Science", "Ecology and Evolutionary Biology", "Polymerase Chain Reaction\u2013Denaturing Gradient Gel Electrophoresis", "Polymerase Chain Reaction", "01 natural sciences", "Soil Microbial Community", "Soil", "Ozone", "Health Sciences", "Acetylglucosaminidase", "Cellular and Developmental Biology", "Ecosystem", "Soil Microbiology", "beta-Glucosidase", "Fatty Acids", "Fungi", "Natural Resources and Environment", "Molecular", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Fungal Metabolism", "Carbon", "Free-air CO 2 and O 3 Enrichment", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Extracellular Space"]}, "links": [{"href": "https://doi.org/10.1007/s00442-005-0249-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-005-0249-3", "name": "item", "description": "10.1007/s00442-005-0249-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-005-0249-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-10-05T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2015.06.044", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:20Z", "type": "Journal Article", "created": "2015-07-02", "title": "Contrasting Effects Of Biochar Versus Manure On Soil Microbial Communities And Enzyme Activities In An Aridisol", "description": "Biochar can increase microbial activity, alter microbial community structure, and increase soil fertility in arid and semi-arid soils, but at relatively high rates that may be impractical for large-scale field studies. This contrasts with organic amendments such as manure, which can be abundant and inexpensive if locally available, and thus can be applied to fields at greater rates than biochar. In a field study comparing biochar and manure, a fast pyrolysis hardwood biochar (22.4 Mg ha(-1)), dairy manure (42 Mg ha(-1) dry wt), a combination of biochar and manure at the aforementioned rates, or no amendment (control) was applied to an Aridisol (n=3) in fall 2008. Plots were annually cropped to corn (Zea maize L.). Surface soils (0-30 cm) were sampled directly under corn plants in late June 2009 and early August 2012, and assayed for microbial community fatty acid methyl ester (FAME) profiles and six extracellular enzyme activities involved in soil C, N, and P cycling. Arbuscular mycorrhizal (AM) fungal colonization was assayed in corn roots in 2012. Biochar had no effect on microbial biomass, community structure, extracellular enzyme activities, or AM fungi root colonization of corn. In the short-term, manure amendment increased microbial biomass, altered microbial community structure, and significantly reduced the relative concentration of the AM fungal biomass in soil. Manure also reduced the percent root colonization of corn by AM fungi in the longer-term. Thus, biochar and manure had contrasting short-term effects on soil microbial communities, perhaps because of the relatively low application rate of biochar.", "keywords": ["2. Zero hunger", "04 agricultural and veterinary sciences", "15. Life on land", "Zea mays", "6. Clean water", "Enzymes", "Manure", "Chemistry", "Soil", "Fertility", "Charcoal", "Mycorrhizae", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Extracellular Space", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2015.06.044"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2015.06.044", "name": "item", "description": "10.1016/j.chemosphere.2015.06.044", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2015.06.044"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-01-01T00:00:00Z"}}, {"id": "10.1111/j.1469-8137.2010.03321.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:17:41Z", "type": "Journal Article", "created": "2010-06-11", "title": "Belowground Carbon Allocation By Trees Drives Seasonal Patterns Of Extracellular Enzyme Activities By Altering Microbial Community Composition In A Beech Forest Soil", "description": "*Plant seasonal cycles alter carbon (C) and nitrogen (N) availability for soil microbes, which may affect microbial community composition and thus feed back on microbial decomposition of soil organic material and plant N availability. The temporal dynamics of these plant-soil interactions are, however, unclear. *Here, we experimentally manipulated the C and N availability in a beech forest through N fertilization or tree girdling and conducted a detailed analysis of the seasonal pattern of microbial community composition and decomposition processes over 2 yr. *We found a strong relationship between microbial community composition and enzyme activities over the seasonal course. Phenoloxidase and peroxidase activities were highest during late summer, whereas cellulase and protease peaked in late autumn. Girdling, and thus loss of mycorrhiza, resulted in an increase in soil organic matter-degrading enzymes and a decrease in cellulase and protease activity. *Temporal changes in enzyme activities suggest a switch of the main substrate for decomposition between summer (soil organic matter) and autumn (plant litter). Our results indicate that ectomycorrhizal fungi are possibly involved in autumn cellulase and protease activity. Our study shows that, through belowground C allocation, trees significantly alter soil microbial communities, which may affect seasonal patterns of decomposition processes.", "keywords": ["Nitrogen", "Climate", "Trees", "Soil", "Mycorrhizae", "401902 Soil science", "Fagus", "Biomass", "Phospholipids", "Soil Microbiology", "106022 Mikrobiologie", "Bacteria", "Research", "Temperature", "04 agricultural and veterinary sciences", "15. 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