{"type": "FeatureCollection", "features": [{"id": "10.1007/s00442-005-0249-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:24Z", "type": "Journal Article", "created": "2005-10-04", "title": "Fungal Community Composition And Metabolism Under Elevated Co2 And O-3", "description": "Atmospheric CO(2) and O(3) concentrations are increasing due to human activity and both trace gases have the potential to alter C cycling in forest ecosystems. Because soil microorganisms depend on plant litter as a source of energy for metabolism, changes in the amount or the biochemistry of plant litter produced under elevated CO(2) and O(3) could alter microbial community function and composition. Previously, we have observed that elevated CO(2) increased the microbial metabolism of cellulose and chitin, whereas elevated O(3) dampened this response. We hypothesized that this change in metabolism under CO(2) and O(3) enrichment would be accompanied by a concomitant change in fungal community composition. We tested our hypothesis at the free-air CO(2) and O(3) enrichment (FACE) experiment at Rhinelander, Wisconsin, in which Populus tremuloides, Betula papyrifera, and Acer saccharum were grown under factorial CO(2) and O(3) treatments. We employed extracellular enzyme analysis to assay microbial metabolism, phospholipid fatty acid (PLFA) analysis to determine changes in microbial community composition, and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to analyze the fungal community composition. The activities of 1,4-beta-glucosidase (+37%) and 1,4,-beta-N-acetylglucosaminidase (+84%) were significantly increased under elevated CO(2), whereas 1,4-beta-glucosidase activity (-25%) was significantly suppressed by elevated O(3). There was no significant main effect of elevated CO(2) or O(3) on fungal relative abundance, as measured by PLFA. We identified 39 fungal taxonomic units from soil using DGGE, and found that O(3) enrichment significantly altered fungal community composition. We conclude that fungal metabolism is altered under elevated CO(2) and O(3), and that there was a concomitant change in fungal community composition under elevated O(3). Thus, changes in plant inputs to soil under elevated CO(2) and O(3) can propagate through the microbial food web to alter the cycling of C in soil.", "keywords": ["0106 biological sciences", "Food Chain", "Extracellular Enzymes", "Science", "Ecology and Evolutionary Biology", "Polymerase Chain Reaction\u2013Denaturing Gradient Gel Electrophoresis", "Polymerase Chain Reaction", "01 natural sciences", "Soil Microbial Community", "Soil", "Ozone", "Health Sciences", "Acetylglucosaminidase", "Cellular and Developmental Biology", "Ecosystem", "Soil Microbiology", "beta-Glucosidase", "Fatty Acids", "Fungi", "Natural Resources and Environment", "Molecular", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Fungal Metabolism", "Carbon", "Free-air CO 2 and O 3 Enrichment", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Extracellular Space"]}, "links": [{"href": "https://doi.org/10.1007/s00442-005-0249-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-005-0249-3", "name": "item", "description": "10.1007/s00442-005-0249-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-005-0249-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-10-05T00:00:00Z"}}, {"id": "10.1002/ppp3.10222", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:05Z", "type": "Journal Article", "created": "2021-08-24", "title": "The emerging threat of human\u2010use antifungals in sustainable and circular agriculture schemes", "description": "Societal Impact Statement

Rapidly growing global populations mandate greater crop productivity despite increasingly scarce natural resources, including freshwater. The adoption of sustainable agricultural practices seek to address such issues, but an unintended consequence is the exposure of agricultural soils and associated biota to emerging contaminants including azole pharmaceutical antifungals. We show that environmentally relevant exposure to three commonly prescribed azole antifungals can reduce mycorrhizal 33P transfer from the soil into the host plant. This suggests that exposure to azoles may have a significant impact on mycorrhizal\uffe2\uff80\uff90mediated transfer of nutrients in soil\uffe2\uff80\uff90plant systems. Understanding the unintended consequences of sustainable agricultural practices is needed to ensure the security and safety of future food production systems.

Summary

Sustainable farming practices are increasingly necessary to meet the demands of a growing population under constraints imposed by climate change. These practices, in particular the reuse of wastewater and amending soil with wastewater derived biosolids, provide a pathway for man\uffe2\uff80\uff90made chemicals to enter the agricultural environment.

Among the chemicals commonly detected in wastewater and biosolids are pharmaceutical azole antifungals. Fungi, in particular mycorrhiza\uffe2\uff80\uff90forming fungal symbionts of plant roots, are key drivers of nutrient cycling in the soil\uffe2\uff80\uff93plant system. As such, greater understanding of the impacts of azole antifungal exposure in agricultural systems is urgently needed.

We exposed wheat (Triticum aestivum L. cv. \uffe2\uff80\uff98Skyfall\uffe2\uff80\uff99) and arbuscular mycorrhizal fungi to environmentally relevant concentrations of three azole antifungals (clotrimazole, miconazole nitrate and fluconazole). We traced the mycorrhizal\uffe2\uff80\uff90acquired 33P from the soil into the host plant in contaminated versus non\uffe2\uff80\uff90contaminated soils and found 33P transfer from mycorrhizal fungi to host plants was reduced in soils containing antifungals. This represents a potentially major disruption to soil nutrient flows as a result of soil contamination.

Our work raises the major issue of exposure of soil biota to pharmaceuticals such as azole antifungals, introduced via sustainable agricultural practices, as a potentially globally important disruptive influence on soil nutrient cycles. The impacts of these compounds on non\uffe2\uff80\uff90target organisms, beneficial mycorrhizal fungi in particular, could have major implications on security and sustainability of future food systems.

Responses of the soil microbial and nematode community to organic and conventional agricultural practices were studied using the Teagasc Kinsealy Systems Comparison trial as the experimental system. The trial is a long\uffe2\uff80\uff90term field experiment which divides conventional and organic agriculture into component pest\uffe2\uff80\uff90control and soil treatment practices. We hypothesised that management practices would affect soil ecology and used community level physiological profiles, microbial and nematode counts, and denaturing gradient gel electrophoresis (DGGE) to characterise soil microbial communities in plots used for onion (Allium cepa L.) cultivation.

RESULTS

Microbial activity and culturable bacterial counts were significantly higher under fully organic management. Culturable fungi, actinomycete and nematode counts showed a consistent trend towards higher numbers under fully organic management but these data were not statistically significant. No differences were found in the fungal/bacterial ratio. DGGE banding patterns and sequencing of excised bands showed clear differences between treatments. Putative onion fungal pathogens were predominantly sequenced under conventional soil treatment practices whilst putative soil suppressive bacterial species were predominantly sequenced from the organic pest\uffe2\uff80\uff90control treatment plots.

CONCLUSION

Organic management increased microbial activity and diversity. Sequence data was indicative of differences in functional groups and warrants further investigation. \uffc2\uffa9 2013 Society of Chemical Industry

", "keywords": ["DNA", " Bacterial", "0301 basic medicine", "Microbial diversity", "Nematoda", "Soil", "03 medical and health sciences", "Onions", "Animals", "DNA", " Fungal", "Ecosystem", "Soil Microbiology", "2. Zero hunger", "Organic Agriculture", "0303 health sciences", "Bacteria", "Denaturing Gradient Gel Electrophoresis", "0402 animal and dairy science", "Fungi", "Denaturing gradient gel electrophoresis", "Agriculture", "Biolog Eco-plates", "04 agricultural and veterinary sciences", "15. Life on land", "Community level physiological profile", "Organic agriculture", "0405 other agricultural sciences"]}, "links": [{"href": "https://doi.org/10.1002/jsfa.6206"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.6206", "name": "item", "description": "10.1002/jsfa.6206", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.6206"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-06-07T00:00:00Z"}}, {"id": "10.1007/bf00010794", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:10Z", "type": "Journal Article", "created": "2006-07-29", "title": "Nutrient And Carbon-Dioxide Release By Interacting Species Of Straw-Decomposing Fungi", "description": "Pairs of fungi were incubated on wheat straw in microcosms for 10 weeks. Release of Na+, K+ and NH4+-N was similar from all combinations, but Ca2+, Mg2+ and PO43--P release depended on the species. In Agrocybe gibberosa/Chaetomium globosum and Sphaerobolus stellatus/Chaetomium globosum combinations, there was evidence of interactions which suppressed the predicted rate of phosphate release, and in all the mixed species combinations there were interactions which increased the rate of fungal respiration above that of the more combative fungus in pure culture. ei]{gnR}{fnMerckx}", "keywords": ["decomposition", "nutrients", "fungal interactions", "straw", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "respiration"], "contacts": [{"organization": "Robinson, Clare H., Dighton, J., Frankland, Juliet C., Coward, P. A.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/bf00010794"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/bf00010794", "name": "item", "description": "10.1007/bf00010794", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/bf00010794"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1993-04-01T00:00:00Z"}}, {"id": "10.1007/s00248-013-0225-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:16Z", "type": "Journal Article", "created": "2013-04-15", "title": "Agricultural Management And Labile Carbon Additions Affect Soil Microbial Community Structure And Interact With Carbon And Nitrogen Cycling", "description": "We investigated how conversion from conventional agriculture to organic management affected the structure and biogeochemical function of soil microbial communities. We hypothesized the following. (1) Changing agricultural management practices will alter soil microbial community structure driven by increasing microbial diversity in organic management. (2) Organically managed soil microbial communities will mineralize more N and will also mineralize more N in response to substrate addition than conventionally managed soil communities. (3) Microbial communities under organic management will be more efficient and respire less added C. Soils from organically and conventionally managed agroecosystems were incubated with and without glucose ((13)C) additions at constant soil moisture. We extracted soil genomic DNA before and after incubation for TRFLP community fingerprinting of soil bacteria and fungi. We measured soil C and N pools before and after incubation, and we tracked total C respired and N mineralized at several points during the incubation. Twenty years of organic management altered soil bacterial and fungal community structure compared to continuous conventional management with the bacterial differences caused primarily by a large increase in diversity. Organically managed soils mineralized twice as much NO3 (-) as conventionally managed ones (44 vs. 23 \u03bcg N/g soil, respectively) and increased mineralization when labile C was added. There was no difference in respiration, but organically managed soils had larger pools of C suggesting greater efficiency in terms of respiration per unit soil C. These results indicate that the organic management induced a change in community composition resulting in a more diverse community with enhanced activity towards labile substrates and greater capacity to mineralize N.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Environmental Microbiology and Microbial Ecology", "Bacteria", "Nitrogen", "Fungal Community Structure", "Agriculture", "Nitrogen Cycle", "15. Life on land", "Microbiology", "630", "Carbon", "Carbon Cycle", "Soil", "03 medical and health sciences", "rRNA Gene Copy", "Soil Microbial Community Structure", "fungal community", "Biology", "Ecosystem", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-013-0225-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-013-0225-0", "name": "item", "description": "10.1007/s00248-013-0225-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-013-0225-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-04-16T00:00:00Z"}}, {"id": "10.1007/s00374-007-0214-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:20Z", "type": "Journal Article", "created": "2007-06-14", "title": "Impact Of Fire On Fungal Abundance And Microbial Efficiency In C Assimilation And Mineralisation In A Mediterranean Maquis Soil", "description": "The present study investigates the impact of fire (low and high severity) on soil fungal abundance and microbial efficiency in C assimilation and mineralisation in a Mediterranean maquis area of Southern Italy over 2 years after fire. In burned and control soils total and active fungal mycelium, microbial biomass C, percentage of microbial biomass C present as fungal C, metabolic quotient (qCO2) and coefficient of endogenous mineralisation (CEM) were assayed together with several chemical properties of soil (i.e. pH and contents of organic C, total and mineral N, available K, Mg, Mn and water). Fire significantly decreased the fungal mycelium, whereas it stimulated microbial growth probably through the enhancement of bacterial growth because of the increase in organic C and nutrient contents in burned plots. This shift in microbial community composition might explain the observed reduction in soil microbial efficiency of C assimilation (high qCO2) and the increase in C mineralisation rate (CEM) in the first 84 days after fire. Therefore, fire might increase CO2 input to the atmosphere not only during combustion phase but also in the post-fire period.", "keywords": ["2. Zero hunger", "CEM; Fire severity; Fungal mycelium; Microbial biomass; qCO; 2;", "fungal mycelium", "fire severity", "microbial biomass", "13. Climate action", "microbial biomass; fungal mycelium; fire severity", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s00374-007-0214-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biology%20and%20Fertility%20of%20Soils", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00374-007-0214-x", "name": "item", "description": "10.1007/s00374-007-0214-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00374-007-0214-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-06-15T00:00:00Z"}}, {"id": "10.1007/s00572-015-0655-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:26Z", "type": "Journal Article", "created": "2015-07-25", "title": "The Ectomycorrhizal Community Of Conifer Stands On Peat Soils 12 Years After Fertilization With Wood Ash", "description": "We studied long-term effects of fertilization with wood ash on biomass, vitality and mycorrhizal colonization of fine roots in three conifer forest stands growing in Vacciniosa turf. mel. (V), Myrtillosa turf. mel. (M) and Myrtillosa turf. mel./Caricoso-phragmitosa (MC) forest types on peat soils. Fertilization trials amounting 5 kg/m(2) of wood ash were established 12 years prior to this study. A total of 63 soil samples with roots were collected and analysed. Ectomycorrhizal (ECM) fungi in roots were identified by morphotyping and sequencing of the fungal internal transcribed spacer (ITS) region. In all forest types, fine root biomass was higher in fertilized plots than in control plots. In M forest type, proportion of living fine roots was greater in fertilized plots than in control plots, while in V and MC, the result was opposite. Fifty ECM species were identified, of which eight were common to both fertilized and control plots. Species richness and Shannon diversity index were generally higher in fertilized plots than in control plots. The most common species in fertilized plots were Amphinema byssoides (17.8%) and Tuber cf. anniae (12.2%), while in control plots, it was Tylospora asterophora (18.5%) and Lactarius tabidus (20.3%). Our results showed that forest fertilization with wood ash has long-lasting effect on diversity and composition of ECM fungal communities.", "keywords": ["0106 biological sciences", "570", "forest fertilization", "m\u00e4nty", "Molecular Sequence Data", "organic soils", "fine roots", "Plant Roots", "01 natural sciences", "630", "mets\u00e4nlannoitus", "Mycorrhizae", "ectomycorrhizae", "DNA", " Ribosomal Spacer", "Muut aihealueet", "DNA", " Fungal", "2. Zero hunger", "Picea abies", "Pinus sylvestris", "Sequence Analysis", " DNA", "04 agricultural and veterinary sciences", "15. Life on land", "Biota", "hienojuuret", "kuusi", "Tracheophyta", "eloper\u00e4iset maat", "0401 agriculture", " forestry", " and fisheries", "ektomykorritsa"]}, "links": [{"href": "https://doi.org/10.1007/s00572-015-0655-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Mycorrhiza", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00572-015-0655-2", "name": "item", "description": "10.1007/s00572-015-0655-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00572-015-0655-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-07-26T00:00:00Z"}}, {"id": "10.1007/s00572-016-0694-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:26Z", "type": "Journal Article", "created": "2016-04-14", "title": "Organic Amendments Increase Phylogenetic Diversity Of Arbuscular Mycorrhizal Fungi In Acid Soil Contaminated By Trace Elements", "description": "In 1998, a toxic mine spill polluted a 55-km(2) area in a basin southward to Do\u00f1ana National Park (Spain). Subsequent attempts to restore those trace element-contaminated soils have involved physical, chemical, or biological methodologies. In this study, the restoration approach included application of different types and doses of organic amendments: biosolid compost (BC) and leonardite (LEO). Twelve years after the last addition, molecular analyses of arbuscular mycorrhizal (AM) fungal communities associated with target plants (Lamarckia aurea and Chrysanthemum coronarium) as well as analyses of trace element concentrations both in soil and in plants were performed. The results showed an improved soil quality reflected by an increase in soil pH and a decrease in trace element availability as a result of the amendments and dosages. Additionally, the phylogenetic diversity of the AM fungal community increased, reaching the maximum diversity at the highest dose of BC. Trace element concentration was considered the predominant soil factor determining the AM fungal community composition. Thereby, the studied AM fungal community reflects a community adapted to different levels of contamination as a result of the amendments. The study highlights the long-term effect of the amendments in stabilizing the soil system.", "keywords": ["2. Zero hunger", "0301 basic medicine", "Minerals", "0303 health sciences", "Bioindicator", "Chrysanthemum", "Genetic Variation", "Hydrogen-Ion Concentration", "15. Life on land", "Poaceae", "Soil biodiversity", "Trace element contaminated soils", "Ecosystem restoration", "Mining", "Soil fungal community", "Trace Elements", "Soil", "03 medical and health sciences", "Biodegradation", " Environmental", "13. Climate action", "Mycorrhizae", "Mine spill", "Bioindicators", "Soil Pollutants", "Phylogeny"]}, "links": [{"href": "https://doi.org/10.1007/s00572-016-0694-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Mycorrhiza", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00572-016-0694-3", "name": "item", "description": "10.1007/s00572-016-0694-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00572-016-0694-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-04-12T00:00:00Z"}}, {"id": "10.1007/s10533-012-9808-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:36Z", "type": "Journal Article", "created": "2012-10-31", "title": "Earthworms, Litter And Soil Carbon In A Northern Hardwood Forest", "description": "The important role of soil carbon (C) in the global C cycle has stimulated interest in better understanding the mechanisms regulating soil C storage and its stabilization. Exotic earthworm invasion of northern forest soils in North America can affect soil C pools, and we examined their effects on these mechanisms by adding 13C labeled leaf litter to adjacent northern hardwood forests with and without earthworms. Two types of labeled litter were produced, one with the 13C more concentrated in structural (S) components and the other in non-structural (NS) components, to evaluate the role of biochemical differences in soil C stabilization. Earthworm invasions have reduced soil C storage in the upper 20\u00a0cm of the soil profile by 37\u00a0%, mostly by eliminating surface organic horizons. Despite rapid mixing of litter into mineral soil and its incorporation into aggregates, mineral soil C has not increased in the presence of earthworms. Incorporation of litter C into soil and microbial biomass was not affected by biochemical differences between S versus NS labeled litter although NS litter C was assimilated more readily into earthworm biomass and S litter C into fungal hyphae. Apparently, the net effect of earthworm mixing of litter and forest floor C into mineral soil, plus stabilization of that C in aggregates, is counterbalanced by earthworm bioturbation and possible priming effects. Our results support recent arguments that biochemical recalcitrance is not a major contributor to the stabilization of soil C.", "keywords": ["IMPACTS", "Decomposition", "STABILIZATION", "Multidisciplinary", "biomass", "MICROBIAL BIOMASS", "INVASION", "Microbial biomass", "TEMPERATE", "04 agricultural and veterinary sciences", "SEQUESTRATION", "15. Life on land", "Fungal hyphae", "Aggregation", "Microbial", "Sugar maple", "FORESTS", "DISSOLVED ORGANIC-MATTER", "PATTERNS", "0401 agriculture", " forestry", " and fisheries", "USA", "Environmental Sciences", "Geosciences", "Isotope labeling", "CENTRAL NEW-YORK"]}, "links": [{"href": "https://doi.org/10.1007/s10533-012-9808-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biogeochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10533-012-9808-y", "name": "item", "description": "10.1007/s10533-012-9808-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10533-012-9808-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-11-01T00:00:00Z"}}, {"id": "10.1016/j.agee.2024.109035", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:15:17Z", "type": "Journal Article", "created": "2024-05-04", "title": "Soil and climatic characteristics and farming system shape fungal communities in European wheat fields", "description": "Fungi play a pivotal role as highly effective decomposers of plant residues and essential mycorrhizal symbionts,\u00a0augmenting water and nutrient uptake in plants and contributing to diverse functions within agroecosystems.\u00a0This study examined soil fungi in 188 wheat fields across nine European pedoclimatic zones under both conventional\u00a0and organic farming systems, utilizing ITS1 amplicon sequencing. The investigation aimed to quantify\u00a0changes induced by the farming system in soil fungi and their correlation with soil features and climatic factors\u00a0across these pedoclimatic zones, spanning from northern to southern Europe. The pedoclimatic zone emerged as\u00a0a key determinant in shaping the overall composition of the fungal community. Zones characterized by moist and\u00a0cool climates, along with low levels of available phosphorus and carbonate, exhibited higher fungal richness.\u00a0However, variations in fungal diversity and relative abundances were observed within zones due to farming\u00a0system-induced changes. Soil pH and bulk density were identified as major factors, for example, they correlate\u00a0with an increase in potential pathogenic taxa (Mycosphaerella, Nectriaceae, Alternaria) in two Mediterranean\u00a0zones and with an increase of potential plant growth promoting taxa (Saitozyma, Solicoccozyma) in the Boreal\u00a0zone. Organic farming, in general, promoted elevated fungal richness. The Lusitanian and Nemoral zones under\u00a0organic farming exhibited the highest fungal richness and diversity. In terms of organic farming, both symbiotrophs\u00a0and potential pathogens increased in the Lusitanian zone, while pathotrophs were more prevalent in the\u00a0Central Atlantic and South Mediterranean zones under organic farming. These findings propose potential indicators\u00a0for organic farming, including fungal endophytes in zones characterized by a moist and cool climate, low\u00a0available phosphorus content, and low soil pH. Organic farming may favor mycorrhizae and potential pathogens\u00a0in zones with drier and warmer climates, along with higher soil pH, calcium carbonate content, and bulk density.\u00a0This study provides novel insights and underscores the significance of regional climatic and edaphic conditions in\u00a0shaping the soil fungal community in different farming systems within European wheat fields. This work was funded by the European Commission Horizon 2020 project SoildiverAgro [grant agreement 817819].", "keywords": ["2. Zero hunger", "570", "Organic farming", "15. Life on land", "630", "conventional farming", "wheat field", "Conventional farming", "organic farming", "Agricultural soils", "farming system", "fungi", "Fungal diversity"]}, "links": [{"href": "https://doi.org/10.1016/j.agee.2024.109035"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2024.109035", "name": "item", "description": "10.1016/j.agee.2024.109035", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2024.109035"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-01T00:00:00Z"}}, {"id": "10.1007/s11104-013-1855-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:50Z", "type": "Journal Article", "created": "2013-08-21", "title": "Soil Microorganisms Respond To Five Years Of Climate Change Manipulations And Elevated Atmospheric Co2 In A Temperate Heath Ecosystem", "description": "Soil microbial responses to global change can affect organic matter turnover and nutrient cycling thereby altering the overall ecosystem functioning. In a large-scale experiment, we investigated the impact of 5\u00a0years of climate change and elevated atmospheric CO2 on soil microorganisms and nutrient availability in a temperate heathland. The future climate was simulated by increased soil temperature (+0.3\u00a0\u00b0C), extended pre-summer drought (excluding 5\u20138\u00a0% of the annual precipitation) and elevated CO2 (+130\u00a0ppm) in a factorial design. Soil organic matter and nutrient pools were analysed and linked to microbial measures by quantitative PCR of bacteria and fungi, chloroform fumigation extraction, and substrate-induced respiration to assess their impact of climate change on nutrient availability. Warming resulted in higher measures of fungi and bacteria, of microbial biomass and of microbial growth potential, however, this did not reduce the availability of nitrogen or phosphorus in the soil. Elevated CO2 did not directly affect the microbial measures or nutrient pools, whereas drought shifted the microbial community towards a higher fungal dominance. Although we were not able to show strong interactive effects of the global change factors, warming and drought changed both nutrient availability and microbial community composition in the heathland soil, which could alter the ecosystem carbon and nutrient flow in the long-term.", "keywords": ["Bacterial abundance", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Soil nutrient pools", "Elevated carbon dioxide", "CLIMAITE", "Fungal abundance", "15. Life on land", "Real-time quantitative PCR", "6. Clean water", "Long-term ecosystem manipulation", "03 medical and health sciences", "13. Climate action", "11. Sustainability", "Deschampsia flexuosa"]}, "links": [{"href": "https://doi.org/10.1007/s11104-013-1855-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11104-013-1855-1", "name": "item", "description": "10.1007/s11104-013-1855-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11104-013-1855-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-08-22T00:00:00Z"}}, {"id": "10.1016/j.jafr.2023.100732", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:16:16Z", "type": "Journal Article", "created": "2023-08-07", "title": "Aeromycological studies in the crops of the main cereals: A systematic review", "description": "Open AccessLes \u00e9tudes a\u00e9romycologiques sur les cultures c\u00e9r\u00e9ali\u00e8res permettent de d\u00e9terminer la variation temporelle des agents pathog\u00e8nes des plantes affectant la culture et de d\u00e9terminer le moment appropri\u00e9 pour appliquer les fongicides. Cependant, ce sujet n'a pas \u00e9t\u00e9 syst\u00e9matiquement revu. L'objectif de ce travail \u00e9tait d'analyser syst\u00e9matiquement toutes les \u00e9tudes a\u00e9romycologiques r\u00e9alis\u00e9es sur le ma\u00efs, le bl\u00e9, le riz, l'avoine, l'orge, le seigle, le sorgho et le millet. Une recherche syst\u00e9matique a \u00e9t\u00e9 effectu\u00e9e dans Scopus depuis le d\u00e9but de la base de donn\u00e9es jusqu'au 1er ao\u00fbt 2022. Les crit\u00e8res d'inclusion \u00e9taient qu'il s'agissait d'\u00e9tudes a\u00e9romycologiques sur le bl\u00e9 ou le riz ou le ma\u00efs ou l'avoine ou le sorgho ou le seigle ou l'orge ou le millet et d'\u00e9tudes publi\u00e9es dans des revues \u00e0 comit\u00e9 de lecture index\u00e9es dans Journal Citation Reports et r\u00e9dig\u00e9es en anglais ou en espagnol. Quarante-trois \u00e9tudes (21 sur le bl\u00e9, 15 sur le riz, 5 sur le ma\u00efs, 1 sur le sorgho et 2 sur l'orge) r\u00e9pondant \u00e0 tous les crit\u00e8res d'\u00e9ligibilit\u00e9 ont \u00e9t\u00e9 incluses (une des \u00e9tudes sur le ma\u00efs a \u00e9galement \u00e9t\u00e9 men\u00e9e sur le bl\u00e9). Aucune \u00e9tude a\u00e9romycologique n'a \u00e9t\u00e9 trouv\u00e9e chez l'avoine, le seigle et le millet. Il a \u00e9t\u00e9 not\u00e9 que la plupart des recherches a\u00e9romycologiques ont \u00e9t\u00e9 men\u00e9es sur les cultures de bl\u00e9 et principalement dans les pays des Am\u00e9riques. De plus, les propagules fongiques sont principalement collect\u00e9es par des m\u00e9thodes non viables, en utilisant divers types de collecteurs. En g\u00e9n\u00e9ral, les \u00e9tudes visaient \u00e0 identifier un agent pathog\u00e8ne sp\u00e9cifique et non \u00e0 la diversit\u00e9 des agents pathog\u00e8nes qui peuvent \u00eatre trouv\u00e9s. La relation des champignons identifi\u00e9s avec les param\u00e8tres m\u00e9t\u00e9orologiques \u00e9tait variable dans les diff\u00e9rentes \u00e9tudes. Cette revue syst\u00e9matique permet de r\u00e9sumer les \u00e9tudes a\u00e9romycologiques qui ont \u00e9t\u00e9 men\u00e9es sur les cultures de bl\u00e9, de riz, de ma\u00efs, de sorgho et d'orge. Il sugg\u00e8re \u00e9galement o\u00f9 les futures \u00e9tudes dans ce domaine devraient \u00eatre dirig\u00e9es, en fonction des limites rencontr\u00e9es.", "keywords": ["Impacts of Elevated CO2 and Ozone on Plant Physiology", "Agriculture (General)", "Health", " Toxicology and Mutagenesis", "Plant Science", "Crop", "S1-972", "Agricultural and Biological Sciences", "Barley", "Biochemistry", " Genetics and Molecular Biology", "TX341-641", "10. No inequality", "Biology", "Sorghum", "2. Zero hunger", "Corn", "Airborne spores", "Nutrition. Foods and food supply", "Life Sciences", "Phylogenetic Analysis", "Cell Biology", "15. Life on land", "2414.06 Hongos", "Agronomy", "3. Good health", "Wheat", "Environmental Science", "Physical Sciences", "Rice", "Indoor Air Quality and Health Effects", "Diversity and Evolution of Fungal Pathogens", "Biotechnology"]}, "links": [{"href": "https://doi.org/10.1016/j.jafr.2023.100732"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Agriculture%20and%20Food%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jafr.2023.100732", "name": "item", "description": "10.1016/j.jafr.2023.100732", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jafr.2023.100732"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-01T00:00:00Z"}}, {"id": "10.1016/j.gecco.2016.03.009", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:16:07Z", "type": "Journal Article", "created": "2016-04-08", "title": "High Quality Residues From Cover Crops Favor Changes In Microbial Community And Enhance C And N Sequestration", "description": "The objective of the study was to evaluate the effect of a change in management on the soil microbial community and C sequestration. We conducted a 3-year field study in La Pampa (Argentina) with rotation of sorghum (Sorghum bicolor) in zero tillage alternating with rye (Secale cereale) and vetch (Vicia villosa ssp. dasycarpa). Soil was sampled once a year at two depths. Soil organic matter fractions, dissolved organic matter, microbial biomass (MBC) and community composition (DNA extraction, qPCR, and phospholipid FAME profiles) were determined. Litter, aerial- and root biomass were collected and all material was analyzed for C and N. Results showed a rapid response of microbial biomass to a bacterial dominance independent of residue quality. Vetch had the highest diversity index, while the fertilized treatment had the lowest one. Vetch\u2013sorghum rotation with high N mineralization rates and diverse microbial community sequestered more C and N in stable soil organic matter fractions than no-till sorghum alone or with rye, which had lower N turnover rates. These results reaffirm the importance of enhanced soil biodiversity for maintaining soil ecosystem functioning and services. The supply of high amounts of N-rich residues as provided by grass\u2013legume cover crops could fulfill this objective.", "keywords": ["Cultivos de Cobertura", "2. Zero hunger", "Microbial diversity", "Ecology", "Plantas de Cobertura", "Nitr\u00f3geno", "Ecolog\u00eda Microbiana", "Nitrogen", "Microbial biomass C and N", "Carb\u00f3n", "Coil", "No-till", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "Microbial Ecology", "Fungal/bacteria ratio", "11. Sustainability", "Particulate C and N", "0401 agriculture", " forestry", " and fisheries", "Mineral associated C and N", "QH540-549.5", "Cover Plants"]}, "links": [{"href": "https://doi.org/10.1016/j.gecco.2016.03.009"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Ecology%20and%20Conservation", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.gecco.2016.03.009", "name": "item", "description": "10.1016/j.gecco.2016.03.009", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.gecco.2016.03.009"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-04-01T00:00:00Z"}}, {"id": "10.1016/j.jbiotec.2023.07.008", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:16:18Z", "type": "Journal Article", "created": "2023-07-25", "title": "Fast and reliable method to estimate global DNA methylation in plants and fungi with high-pressure liquid chromatography (HPLC)-ultraviolet detection and even more sensitive one with HPLC-mass spectrometry", "description": "DNA (Deoxyribonucleic acid) methylation is one of the epigenetic modifications of DNA, acting as a bridge between genotype and phenotype. Thus, disruption of DNA methylation pattern has tremendous consequences for organism development. Current methods to determine DNA methylation suffer from methodological drawbacks like high requirement of DNA and poor reproducibility of chromatograms. Here we provide a fast and reliable method using high-pressure liquid chromatography (HPLC)-ultraviolet (UV) detector and even more sensitive one with HPLC- mass spectrometry (MS) and we test this method with various plant and fungal DNA isolates. We optimized the preparation of the DNA degradation step to decrease background noise, we improved separation conditions to provide reliable and reproducible chromatograms and conditions to measure nucleotides in HPLC-MS. We showed that global DNA methylation level can be accurately and reproducibly measured with as little as 0.2\u00a0\u00b5M for HPLC-UV and 0.02\u00a0\u00b5M for HPLC-MS of methylated cytosine.", "keywords": ["Chromatography", "Plant DNA", "DNA methylation", "ta1183", "ta1182", "Fungi", "610", "Reproducibility of Results", "DNA Methylation", "Mass Spectrometry", "Fungal DNA", "chromatography", "DNA", " Fungal", "ta116", "Chromatography", " High Pressure Liquid"]}, "links": [{"href": "https://doi.org/10.1016/j.jbiotec.2023.07.008"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jbiotec.2023.07.008", "name": "item", "description": "10.1016/j.jbiotec.2023.07.008", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jbiotec.2023.07.008"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-09-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2009.08.045", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:16:31Z", "type": "Journal Article", "created": "2009-09-23", "title": "Application Of Temporal Temperature Gradient Gel Electrophoresis For Characterisation Of Fungal Endophyte Communities Of Salix Caprea L. In A Heavy Metal Polluted Soil", "description": "Fungal endophytes can affect the heavy metal uptake of their host plants and increase the tolerance of their host plants to heavy metal stress. Therefore, in the present study, a wide-range screening of the fungal endophyte communities was conducted to determine the fungal distribution and diversity on S. caprea roots on a metal polluted site. Fungal communities were screened using amplification with the 5.8S-ITS2-28S part of the rDNA operon, with the resulting amplicons analysed by temporal temperature gradient gel electrophoresis (TTGE) and sequencing. This technique is reproducible and shows good coverage of ascomycete and basidiomycete taxa, as 68% and 32% of all of the sequences, respectively. No clear shift in fungal ITS-TTGE profiles from S. caprea roots was seen along the secondary succession stages. Ascomycetes dominated the more polluted plots, while there was a greater diversity of basidiomycetes in the less polluted and control plots, suggesting greater tolerance of ascomycetes in comparison with basidiomycete fungi. The high diversity of DSEs was confirmed at the highly metal-enriched locations, with species belonging to the genera Phialophora, Phialocephala and Leptodontidium. Furthermore, the DSE colonisation of S. caprea roots and the frequency of the sequences showing affinity towards DSE genus Phialophora, showed good correspondence with soil Pb, Cd and plant-available P concentrations, possibly indicating that DSEs improve metal tolerance of willows to high heavy metal contamination.", "keywords": ["Electrophoresis", " Agar Gel", "0301 basic medicine", "dark septate endophytes", "Fungi", "Temperature", "mycorrhiza", "Salix", "04 agricultural and veterinary sciences", "info:eu-repo/classification/udc/581", "15. Life on land", "heavy metal pollution", "community fingerprinting", "Soil", "03 medical and health sciences", "13. Climate action", "Metals", " Heavy", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "DNA", " Fungal", "Phylogeny", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2009.08.045"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2009.08.045", "name": "item", "description": "10.1016/j.scitotenv.2009.08.045", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2009.08.045"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-12-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2019.107521", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:16:50Z", "type": "Journal Article", "created": "2019-06-26", "title": "Soil multifunctionality is affected by the soil environment and by microbial community composition and diversity", "description": "Microorganisms are critical in mediating carbon (C) and nitrogen (N) cycling processes in soils. Yet, it has long been debated whether the processes underlying biogeochemical cycles are affected by the composition and diversity of the soil microbial community or not. The composition and diversity of soil microbial communities can be influenced by various environmental factors, which in turn are known to impact biogeochemical processes. The objectives of this study were to test effects of multiple edaphic drivers individually and represented as the multivariate soil environment interacting with microbial community composition and diversity, and concomitantly on multiple soil functions (i.e. soil enzyme activities, soil C and N processes). We employed high-throughput sequencing (Illumina MiSeq) to analyze bacterial/archaeal and fungal community composition by targeting the 16S rRNA gene and the ITS1 region of soils collected from three land uses (cropland, grassland and forest) deriving from two bedrock forms (silicate and limestone). Based on this data set we explored single and combined effects of edaphic variables on soil microbial community structure and diversity, as well as on soil enzyme activities and several soil C and N processes. We found that both bacterial/archaeal and fungal communities were shaped by the same edaphic factors, with most single edaphic variables and the combined soil environment representation exerting stronger effects on bacterial/archaeal communities than on fungal communities, as demonstrated by (partial) Mantel tests. We also found similar edaphic controls on the bacterial/archaeal/fungal richness and diversity. Soil C processes were only directly affected by the soil environment but not affected by microbial community composition. In contrast, soil N processes were significantly related to bacterial/archaeal community composition and bacterial/archaeal/fungal richness/diversity but not directly affected by the soil environment. This indicates direct control of the soil environment on soil C processes and indirect control of the soil environment on soil N processes by structuring the microbial communities. The study further highlights the importance of edaphic drivers and microbial communities (i.e. composition and diversity) on important soil C and N processes.", "keywords": ["0301 basic medicine", "570", "550", "ECOSYSTEM MULTIFUNCTIONALITY", "BACTERIAL COMMUNITY", "106027 \u00d6kotoxikologie", "FUNGAL COMMUNITIES", "Soil functions", "Article", "03 medical and health sciences", "Microbial community composition and diversity", "CARBON-USE EFFICIENCY", "106027 Ecotoxicology", "ENZYME-ACTIVITIES", "SDG 15 \u2013 Leben an Land", "Life Below Water", "SDG 15 - Life on Land", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "Agricultural and Veterinary Sciences", "LAND-USE", "SUBSTRATE USE EFFICIENCY", "Agronomy & Agriculture", "Biological Sciences", "15. Life on land", "TEMPERATE FOREST", "13. Climate action", "LONG-TERM N", "106022 Microbiology", "Edaphic drivers", "BAYESIAN CLASSIFIER", "Environmental Sciences"]}, "links": [{"href": "https://escholarship.org/content/qt83b3006k/qt83b3006k.pdf"}, {"href": "https://doi.org/10.1016/j.soilbio.2019.107521"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2019.107521", "name": "item", "description": "10.1016/j.soilbio.2019.107521", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2019.107521"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-09-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2016.03.008", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:16:49Z", "type": "Journal Article", "created": "2016-03-26", "title": "Soil microbial carbon use efficiency and biomass turnover in a long-term fertilization experiment in a temperate grassland", "description": "

Soil microbial carbon use efficiency (CUE), defined as the ratio of organic C allocated to growth over organic C taken up, strongly affects soil carbon (C) cycling. Despite the importance of the microbial CUE for the terrestrial C cycle, very little is known about how it is affected by nutrient availability. Therefore, we studied microbial CUE and microbial biomass turnover time in soils of a long-term fertilization experiment in a temperate grassland comprising five treatments (control, PK, NK, NP, NPK). Microbial CUE and the turnover of microbial biomass were determined using a novel substrate-independent method based on incorporation of 18O from labeled water into microbial DNA. Microbial respiration was 28-37% smaller in all three N treatments (NK, NP, and NPK) compared to the control, whereas the PK treatment did not affect microbial respiration. N-fertilization decreased microbial C uptake, while the microbial growth rate was not affected. Microbial CUE ranged between 0.31 and 0.45, and was 1.3- to 1.4-fold higher in the N-fertilized soils than in the control. The turnover time ranged between 80 and 113 days and was not significantly affected by fertilization. Net primary production (NPP) and the abundance of legumes differed strongly across the treatments, and the fungal:bacterial ratio was very low in all treatments. Structural equation modeling revealed that microbial CUE was exclusively controlled by N fertilization and that neither the abundance of legumes (as a proxy for the quality of the organic matter inputs) nor NPP (as a proxy for C inputs) had an effect on microbial CUE. Our results show that N fertilization did not only decrease microbial respiration, but also microbial C uptake, indicating that less C was intracellularly processed in the N fertilized soils. The reason for reduced C uptake and increased CUE in the N-fertilization treatments is likely an inhibition of oxidative enzymes involved in the degradation of aromatic compounds by N in combination with a reduced energy requirement for microbial N acquisition in the fertilized soils. In conclusion, the study shows that N availability can control soil C cycling by affecting microbial CUE, while plant community-mediated changes in organic matter inputs and P and K availability played no important role for C partitioning of the microbial community in this temperate grassland.

", "keywords": ["FUNGAL", "2. Zero hunger", "106022 Mikrobiologie", "Nitrogen addition", "BACTERIAL", "NITROGEN DEPOSITION", "GROWTH EFFICIENCY", "FOREST FLOOR", "Nutrients", "04 agricultural and veterinary sciences", "15. Life on land", "Stoichiometry", "ORGANIC-MATTER", "RESPIRATION", "106026 \u00d6kosystemforschung", "13. Climate action", "Nutrient limitation", "Microbial growth yield", "106022 Microbiology", "0401 agriculture", " forestry", " and fisheries", "Mean residence time", "STOICHIOMETRIC CONTROLS", "ENZYME-ACTIVITY", "106026 Ecosystem research", "COMMUNITY STRUCTURE"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2016.03.008"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2016.03.008", "name": "item", "description": "10.1016/j.soilbio.2016.03.008", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2016.03.008"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-06-01T00:00:00Z"}}, {"id": "10.1038/ismej.2007.89", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:17:28Z", "type": "Journal Article", "created": "2007-10-25", "title": "The Effect Of Experimental Warming On The Root-Associated Fungal Community Of Salix Arctica", "description": "Abstract

The effect of experimental warming on the root-associated fungal community of arctic willow (Salix arctica) was studied in three distinct habitats at a tundra site in the Canadian High Arctic. Plots were passively warmed for 5\uffe2\uff80\uff937 years using open-top chambers and compared to control plots at ambient temperature. Fungal communities were assessed using terminal restriction fragment length polymorphisms. We found the following: (1) the root-associated fungal community in these high arctic tundra habitats is highly diverse; (2) site and soil characteristics are the most important drivers of community structure and (3) warming increased the density of different genotypes on individual root sections but has not (yet) affected the composition, richness or evenness of the community. The change in genotype density in the warmed plots was associated with an increase in PCR amplification efficiency, suggesting that increased C allocation belowground is increasing the overall biomass of the fungal community.

", "keywords": ["0106 biological sciences", "0301 basic medicine", "Canada", "Fungi", "Salix", "15. Life on land", "Plant Roots", "01 natural sciences", "Heating", "03 medical and health sciences", "Cluster Analysis", "DNA", " Fungal", "Ecosystem", "Polymorphism", " Restriction Fragment Length", "Soil Microbiology"], "contacts": [{"organization": "Gregory H. R. Henry, Keith N. Egger, Kei E. Fujimura,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1038/ismej.2007.89"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/The%20ISME%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/ismej.2007.89", "name": "item", "description": "10.1038/ismej.2007.89", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/ismej.2007.89"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-10-25T00:00:00Z"}}, {"id": "10.1038/nature02053", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:17:29Z", "type": "Journal Article", "created": "2003-10-22", "title": "Genome-Scale Approaches To Resolving Incongruence In Molecular Phylogenies", "description": "One of the most pervasive challenges in molecular phylogenetics is the incongruence between phylogenies obtained using different data sets, such as individual genes. To systematically investigate the degree of incongruence, and potential methods for resolving it, we screened the genome sequences of eight yeast species and selected 106 widely distributed orthologous genes for phylogenetic analyses, singly and by concatenation. Our results suggest that data sets consisting of single or a small number of concatenated genes have a significant probability of supporting conflicting topologies. By contrast, analyses of the entire data set of concatenated genes yielded a single, fully resolved species tree with maximum support. Comparable results were obtained with a concatenation of a minimum of 20 genes; substantially more genes than commonly used but a small fraction of any genome. These results have important implications for resolving branches of the tree of life.", "keywords": ["0301 basic medicine", "Saccharomyces", "0303 health sciences", "03 medical and health sciences", "Genes", " Fungal", "Regression Analysis", "Reproducibility of Results", "Genomics", "Genome", " Fungal", "Phylogeny"]}, "links": [{"href": "https://doi.org/10.1038/nature02053"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/nature02053", "name": "item", "description": "10.1038/nature02053", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/nature02053"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2003-10-01T00:00:00Z"}}, {"id": "10.1038/s41467-018-05980-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:17:31Z", "type": "Journal Article", "created": "2018-08-29", "title": "Land use driven change in soil pH affects microbial carbon cycling processes", "description": "Abstract

Soil microorganisms act as gatekeepers for soil\uffe2\uff80\uff93atmosphere carbon exchange by balancing the accumulation and release of soil organic matter. However, poor understanding of the mechanisms responsible hinders the development of effective land management strategies to enhance soil carbon storage. Here we empirically test the link between microbial ecophysiological traits and topsoil carbon content across geographically distributed soils and land use contrasts. We discovered distinct pH controls on microbial mechanisms of carbon accumulation. Land use intensification in low-pH soils that increased the pH above a threshold (~6.2) leads to carbon loss through increased decomposition, following alleviation of acid retardation of microbial growth. However, loss of carbon with intensification in near-neutral pH soils was linked to decreased microbial biomass and reduced growth efficiency that was, in turn, related to trade-offs with stress alleviation and resource acquisition. Thus, less-intensive management practices in near-neutral pH soils have more potential for carbon storage through increased microbial growth efficiency, whereas in acidic soils, microbial growth is a bigger constraint on decomposition rates.Despite having key functions in terrestrial ecosystems, information on the dominant soil fungi and their ecological preferences at the global scale is lacking. To fill this knowledge gap, we surveyed 235 soils from across the globe. Our findings indicate that 83 phylotypes (<0.1% of the retrieved fungi), mostly belonging to wind dispersed, generalist Ascomycota, dominate soils globally. We identify patterns and ecological drivers of dominant soil fungal taxa occurrence, and present a map of their distribution in soils worldwide. Whole-genome comparisons with less dominant, generalist fungi point at a significantly higher number of genes related to stress-tolerance and resource uptake in the dominant fungi, suggesting that they might be better in colonising a wide range of environments. Our findings constitute a major advance in our understanding of the ecology of fungi, and have implications for the development of strategies to preserve them and the ecosystem functions they provide.Saprobic soil fungi drive many important ecosystem processes, including decomposition, and many of their effects are related to growth rate and enzymatic ability. In mycology, there has long been the implicit assumption of a trade-off between growth and enzymatic investment, which we test here using a set of filamentous fungi from the same soil. For these fungi we measured growth rate (as colony radial extension) and enzymatic repertoire (activities of four enzymes: laccase, cellobiohydrolase, leucine aminopeptidase and acid phosphatase), and explored the interaction between the traits based on phylogenetically corrected methods. Our results support the existence of a trade-off, however only for the enzymes presumably representing a larger metabolic cost (laccase and cellobiohydrolase). Our study offers new insights into potential functional complementarity within the soil fungal community in ecosystem processes, and experimentally supports an enzymatic investment/growth rate trade-off underpinning phenomena including substrate succession.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::579 Mikroorganismen", " Pilze", " Algen", "Acid Phosphatase", "Laccase", "Fungi", "579", "15. Life on land", "Article", "Fungal Proteins", "Microbial ecology", "Leucyl Aminopeptidase", "03 medical and health sciences", "Cellulose 1", "4-beta-Cellobiosidase", "Fungal ecology", "Ecosystem", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://www.biorxiv.org/content/10.1101/360511v1.full.pdf"}, {"href": "https://www.nature.com/articles/s41598-020-68099-8.pdf"}, {"href": "https://doi.org/10.1038/s41598-020-68099-8"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41598-020-68099-8", "name": "item", "description": "10.1038/s41598-020-68099-8", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41598-020-68099-8"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-02T00:00:00Z"}}, {"id": "10.1038/s42003-022-04178-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:17:37Z", "type": "Journal Article", "created": "2022-11-17", "title": "Both abundant and rare fungi colonizing Fagus sylvatica ectomycorrhizal root-tips shape associated bacterial communities", "description": "Abstract

Ectomycorrhizal fungi live in close association with their host plants and form complex interactions with bacterial/archaeal communities in soil. We investigated whether abundant or rare ectomycorrhizal fungi on root-tips of young beech trees (Fagus sylvatica) shape bacterial/archaeal communities. We sequenced 16S rRNA genes and fungal internal transcribed spacer regions of individual root-tips and used ecological networks to detect the tendency of certain assemblies of fungal and bacterial/archaeal taxa to inhabit the same root-tip (i.e. modularity). Individual ectomycorrhizal root-tips hosted distinct fungal communities associated with unique bacterial/archaeal communities. The structure of the fungal-bacterial/archaeal association was determined by both, dominant and rare fungi. Integrating our data in a conceptual framework suggests that the effect of rare fungi on the bacterial/archaeal communities of ectomycorrhizal root-tips contributes to assemblages of bacteria/archaea on root-tips. This highlights the potential impact of complex fine-scale interactions between root-tip associated fungi and other soil microorganisms for the ectomycorrhizal symbiosis.Colonization of terrestrial environments by filamentous fungi relies on their ability to form networks that can forage for and connect resource patches. Despite the importance of these networks, ecologists rarely consider network features as functional traits because their measurement and interpretation are conceptually and methodologically difficult. To address these challenges, we have developed a pipeline to translate images of fungal mycelia, from both micro- and macro-scales, to weighted network graphs that capture ecologically relevant fungal behaviour. We focus on four properties that we hypothesize determine how fungi forage for resources, specifically: connectivity; relative construction cost; transport efficiency; and robustness against attack by fungivores. Constrained ordination and Pareto front analysis of these traits revealed that foraging strategies can be distinguished predominantly along a gradient of connectivity for micro- and macro-scale mycelial networks that is reminiscent of the qualitative \uffe2\uff80\uff98phalanx\uffe2\uff80\uff99 and \uffe2\uff80\uff98guerilla\uffe2\uff80\uff99 descriptors previously proposed in the literature. At one extreme are species with many inter-connections that increase the paths for multidirectional transport and robustness to damage, but with a high construction cost; at the other extreme are species with an opposite phenotype. Thus, we propose this approach represents a significant advance in quantifying ecological strategies for fungi using network information.

", "keywords": ["Microbial ecology", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::579 Mikroorganismen", " Pilze", " Algen", "579", "15. Life on land", "Fungal ecology", "ecological strategies", "Article"]}, "links": [{"href": "https://orca.cardiff.ac.uk/id/eprint/146532/1/Aguilar-Trigueros_et_al-2022-ISME_Communications.pdf"}, {"href": "https://www.nature.com/articles/s43705-021-00085-1.pdf"}, {"href": "https://doi.org/10.1038/s43705-021-00085-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ISME%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s43705-021-00085-1", "name": "item", "description": "10.1038/s43705-021-00085-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s43705-021-00085-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-05T00:00:00Z"}}, {"id": "10.1111/gcb.15420", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:34Z", "type": "Journal Article", "created": "2021-03-04", "title": "Microbial inputs at the litter layer translate climate into altered organic matter properties", "description": "

&lt;p&gt;Plant litter chemistry is altered during decomposition but it remains unknown if these alterations, and thus the composition of residual litter, will change in response to climate. Selective microbial mineralization of litter components and the accumulation of microbial necromass can drive litter compositional change, but the extent to which these mechanisms respond to climate remains poorly understood. We addressed this knowledge gap by studying needle litter decomposition along a boreal forest climate transect. Specifically, we investigated how the composition and/or metabolism of the decomposer community varies with climate, and if that variation is associated with distinct modifications of litter chemistry during decomposition. We analyzed the composition of microbial phospholipid fatty acids (PLFAs) in the litter layer and measured natural abundance &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C&lt;sub&gt;PLFA&lt;/sub&gt; values as an integrated measure of microbial metabolisms. Changes in litter chemistry and &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C values were measured in litterbag experiments conducted at each transect site. A warmer climate was associated with higher litter nitrogen concentrations as well as altered microbial community structure (lower fungi:bacteria ratios) and microbial metabolism (higher &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C&lt;sub&gt;PLFA&lt;/sub&gt;). Litter in warmer transect regions accumulated less aliphatic&amp;#8208;C (lipids, waxes) and retained more O&amp;#8208;alkyl&amp;#8208;C (carbohydrates), consistent with enhanced &lt;sup&gt;13&lt;/sup&gt;C&amp;#8208;enrichment in residual litter, than in colder regions. These results suggest that chemical changes during litter decomposition will change with climate, driven primarily by indirect climate effects (e.g., greater nitrogen availability and decreased fungi:bacteria ratios) rather than direct temperature effects. A positive correlation between microbial biomass &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C values and &lt;sup&gt;13&lt;/sup&gt;C&amp;#8208;enrichment during decomposition suggests that change in litter chemistry is driven more by distinct microbial necromass inputs than differences in the selective removal of litter components. Our study highlights the role that microbial inputs during early litter decomposition can play in shaping surface litter contribution to soil organic matter as it responds to climate warming effects such as greater nitrogen availability.&lt;/p&gt;

", "keywords": ["DECOMPOSITION", "C-13", "CP‐", "necromass", "litter decomposition", "COMMUNITY COMPOSITION", "Soil", "CARBON SEQUESTRATION", "Taiga", "boreal forest", "bacteria", "C-13 NMR", "TEMPERATURE", "Biochemistry", " cell and molecular biology", "Soil Microbiology", "FUNGAL", "2. Zero hunger", "MAS C-13‐", "Fungi", "04 agricultural and veterinary sciences", "15. Life on land", "NMR", "6. Clean water", "climate transect", "Plant Leaves", "13. Climate action", "FOREST SOILS", "PLFA", "0401 agriculture", " forestry", " and fisheries", "fungi", "FATTY-ACIDS", "BULK CARBON", "LIGNIN"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.15420"}, {"href": "https://doi.org/10.1111/gcb.15420"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.15420", "name": "item", "description": "10.1111/gcb.15420", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.15420"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-11-16T00:00:00Z"}}, {"id": "10.1093/femsec/fiab059", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:12Z", "type": "Journal Article", "created": "2021-04-14", "title": "Fungus-bacterium associations are widespread in fungal cultures isolated from a semi-arid natural grassland in Germany", "description": "ABSTRACT

We report on a study that aimed at establishing a large soil\uffe2\uff80\uff93fungal culture collection spanning a wide taxonomic diversity and systematically screening the collection for bacterial associations. Fungal cultures were isolated from soil samples obtained from a natural grassland in eastern Germany and bacterial associations were assessed by PCR-amplification and sequencing of bacterial 16S rRNA. In addition, intraspecies genetic diversities of a subset of the isolated species were estimated by double-digest restriction associated DNA sequencing. A total of 688 fungal cultures, representing at least 106 fungal species from 36 different families, were obtained and even though clonal isolates were identified in almost all fungal species subjected to ddRAD-seq, relatively high genetic diversities could be observed in some of the isolated species. A total of 69% of the fungal isolates in our collection were found to be associated with bacteria and the most commonly identified bacterial genera were Pelomonas, Enterobacter and Burkholderia. Our results indicate that bacterial associations commonly occur in soil fungi, even if antibiotics are being applied during the isolation process, and provide a basis for the use of our culture collection in ecological experiments that want to acknowledge the importance of intraspecies genetic diversity.

", "keywords": ["DNA", " Bacterial", "0301 basic medicine", "bepress|Life Sciences|Ecology and Evolutionary Biology|Terrestrial and Aquatic Ecology", "0303 health sciences", "Terrestrial and Aquatic Ecology", "Bacteria", "Ecology and Evolutionary Biology", "Fungi", "Life Sciences", "15. Life on land", "bepress|Life Sciences|Ecology and Evolutionary Biology", "Grassland", "Soil", "03 medical and health sciences", "bepress|Life Sciences", "Germany", "RNA", " Ribosomal", " 16S", "Humans", "Bacteria ; Fungal-bacterial Interaction ; Soil Fungi ; Ddrad Sequencing", "Soil Microbiology"]}, "links": [{"href": "https://academic.oup.com/femsec/article-pdf/97/5/fiab059/37624504/fiab059.pdf"}, {"href": "https://doi.org/10.1093/femsec/fiab059"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/femsec/fiab059", "name": "item", "description": "10.1093/femsec/fiab059", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/femsec/fiab059"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-04-06T00:00:00Z"}}, {"id": "10.1093/femsle/fnab100", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:12Z", "type": "Journal Article", "created": "2021-07-30", "title": "Transcriptomic markers of fungal growth, respiration and carbon-use efficiency", "description": "ABSTRACT

Fungal metabolic carbon acquisition and its subsequent partitioning between biomass production and respiration, i.e. the carbon-use efficiency (CUE), are central parameters in biogeochemical modeling. However, current available techniques for estimating these parameters are all associated with practical and theoretical shortcomings, making assessments unreliable. Gene expression analyses hold the prospect of phenotype prediction by indirect means, providing new opportunities to obtain information about metabolic priorities. We cultured four different fungal isolates (Chalara longipes, Laccaria bicolor, Serpula lacrymans and Trichoderma harzianum) in liquid media with contrasting nitrogen availability and measured growth rates and respiration to calculate CUE. By relating gene expression markers to measured carbon fluxes, we identified genes coding for 1,3-\uffce\uffb2-glucan synthase and 2-oxoglutarate dehydrogenase as suitable markers for growth and respiration, respectively, capturing both intraspecific variation as well as within-strain variation dependent on growth medium. A transcript index based on these markers correlated significantly with differences in CUE between the fungal isolates. Our study paves the way for the use of these markers to assess differences in growth, respiration and CUE in natural fungal communities, using metatranscriptomic or the RT-qPCR approach.The dependency of microbial activity on nutrient availability in soil is only partly understood, but highly relevant for nutrient cycling dynamics. In order to achieve more insight on microbial adaptations to nutrient limiting conditions, precise physiological knowledge is needed. Therefore, we developed an experimental system assessing traits of 16 saprobic fungal isolates in nitrogen (N) limited conditions. We tested the hypotheses that (1) fungal traits are negatively affected by N deficiency to a similar extent and (2) fungal isolates respond in a phylogenetically conserved fashion. Indeed, mycelial density, spore production and fungal activity (respiration and enzymatic activity) responded similarly to limiting conditions by an overall linear decrease. By contrast, mycelial extension and hyphal elongation peaked at lowest N supply (C:N 200), causing maximal biomass production at intermediate N contents. Optimal N supply rates differed among isolates, but only the extent of growth reduction was phylogenetically conserved. In conclusion, growth responses appeared as a switch from explorative growth in low nutrient conditions to exploitative growth in nutrient\uffe2\uff80\uff90rich patches, as also supported by responses to phosphorus and carbon limitations. This detailed trait\uffe2\uff80\uff90based pattern will not only improve fungal growth models, but also may facilitate interpretations of microbial responses observed in field studies.

", "keywords": ["0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "Nitrogen", "Fungi", "577", "Phosphorus", "Nutrients", "Spores", " Fungal", "15. Life on land", "microbial activity", "01 natural sciences", "Carbon", "Soil", "fungal adaptations", "03 medical and health sciences", "Biomass", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::577 \u00d6kologie", "Soil Microbiology", "nutrient\u2010limiting conditions"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15132"}, {"href": "https://doi.org/10.1111/1462-2920.15132"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1462-2920.15132", "name": "item", "description": "10.1111/1462-2920.15132", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1462-2920.15132"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-07-08T00:00:00Z"}}, {"id": "10.1111/1462-2920.16012", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:27Z", "type": "Journal Article", "created": "2022-04-19", "title": "Soil fungi invest into asexual sporulation under resource scarcity, but trait spaces of individual isolates are unique", "description": "Summary

During the last few decades, a plethora of sequencing studies provided insight into fungal community composition under various environmental conditions. Still, the mechanisms of species assembly and fungal spread in soil remain largely unknown. While mycelial growth patterns are studied extensively, the abundant formation of asexual spores is often overlooked, though representing a substantial part of the fungal life cycle relevant for survival and dispersal. Here, we explore asexual sporulation (spore abundance, size and shape) in 32 co\uffe2\uff80\uff90occurring soil fungal isolates under varying resource conditions, to answer the question whether resource limitation triggers or inhibits fungal investment into reproduction. We further hypothesized that trade\uffe2\uff80\uff90offs exist in fungal investment towards growth, spore production and size. The results revealed overall increased fungal investment into spore production under resource limitations; however, effect sizes and response types varied strongly among fungal isolates. Such isolate\uffe2\uff80\uff90specific effects were apparent in all measured traits, resulting in unique trait spaces of individual isolates. This comprehensive dataset also elucidated variability in sporulation strategies and trade\uffe2\uff80\uff90offs with fungal growth and reproduction under resource scarcity, as only predicted by theoretical models before. The observed isolate\uffe2\uff80\uff90specific strategies likely underpin mechanisms of co\uffe2\uff80\uff90existence in this diverse group of saprobic soil fungi.

", "keywords": ["0106 biological sciences", "570", "ymp\u00e4rist\u00f6tekij\u00e4t", "Reproduction", "Fungi", "1. No poverty", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie", "maaper\u00e4eli\u00f6st\u00f6", "Spores", " Fungal", "15. Life on land", "lis\u00e4\u00e4ntyminen", "01 natural sciences", "Soil", "fungal spread", "Phenotype", "fungal community composition", "Reproduction", " Asexual", "soil fungi", "suvuton lis\u00e4\u00e4ntyminen", "sienet", "iti\u00f6t", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.16012"}, {"href": "https://doi.org/10.1111/1462-2920.16012"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1462-2920.16012", "name": "item", "description": "10.1111/1462-2920.16012", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1462-2920.16012"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-04-27T00:00:00Z"}}, {"id": "10.1111/ele.13632", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:31Z", "type": "Journal Article", "created": "2020-08-11", "title": "Soil fungal mycelia have unexpectedly flexible stoichiometric C:N and C:P ratios", "description": "Abstract

Soil ecological stoichiometry provides powerful theories to integrate the complex interplay of element cycling and microbial communities into biogeochemical models. One essential assumption is that microbes maintain stable C:N:P (carbon:nitrogen:phosphorus) ratios independent of resource supply, although such homeostatic regulations have rarely been assessed in individual microorganisms. Here, we report an unexpected high flexibility in C:N and C:P values of saprobic fungi along nutrient supply gradients, overall ranging between 7\uffe2\uff80\uff90126 and 20\uffe2\uff80\uff901488, respectively, questioning microbial homeostasis. Fungal N:P varied comparatively less due to simultaneous reductions in mycelial N and P contents. As a mechanism, internal recycling processes during mycelial growth and an overall reduced N and P uptake appear more relevant than element storage. The relationships among fungal stoichiometry and growth disappeared in more complex media. These findings affect our interpretation of stoichiometric imbalances among microbes and soils and are highly relevant for developing microbial soil organic carbon and nitrogen models.

", "keywords": ["saprobic fungi", "0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "570", "fungal nutrient retranslocation", "Nitrogen", "nutrient limitations", "microbial carbon sequestration", "Phosphorus", "500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie", "15. Life on land", "01 natural sciences", "Carbon", "soil ecological stoichiometry", "Soil", "element homeostasis", "03 medical and health sciences", "13. Climate action", "mycelial growth", "C:N:P ratios", "Soil Microbiology"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/ele.13632"}, {"href": "https://doi.org/10.1111/ele.13632"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20Letters", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/ele.13632", "name": "item", "description": "10.1111/ele.13632", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/ele.13632"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-08-11T00:00:00Z"}}, {"id": "10.1111/gcb.15722", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:35Z", "type": "Journal Article", "created": "2021-05-24", "title": "Reindeer control over subarctic treeline alters soil fungal communities with potential consequences for soil carbon storage", "description": "Abstract

The climate\uffe2\uff80\uff90driven encroachment of shrubs into the Arctic is accompanied by shifts in soil fungal communities that could contribute to a net release of carbon from tundra soils. At the same time, arctic grazers are known to prevent the establishment of deciduous shrubs and, under certain conditions, promote the dominance of evergreen shrubs. As these different vegetation types associate with contrasting fungal communities, the belowground consequences of climate change could vary among grazing regimes. Yet, at present, the impact of grazing on soil fungal communities and their links to soil carbon have remained speculative. Here we tested how soil fungal community composition, diversity and function depend on tree vicinity and long\uffe2\uff80\uff90term reindeer grazing regime and assessed how the fungal communities relate to organic soil carbon stocks in an alpine treeline ecotone in Northern Scandinavia. We determined soil carbon stocks and characterized soil fungal communities directly underneath and >3\uffc2\uffa0m away from mountain birches (Betula pubescens ssp. czerepanovii) in two adjacent 55\uffe2\uff80\uff90year\uffe2\uff80\uff90old grazing regimes with or without summer grazing by reindeer (Rangifer tarandus). We show that the area exposed to year\uffe2\uff80\uff90round grazing dominated by evergreen dwarf shrubs had higher soil C:N ratio, higher fungal abundance and lower fungal diversity compared with the area with only winter grazing and higher abundance of mountain birch. Although soil carbon stocks did not differ between the grazing regimes, stocks were positively associated with root\uffe2\uff80\uff90associated ascomycetes, typical to the year\uffe2\uff80\uff90round grazing regime, and negatively associated with free\uffe2\uff80\uff90living saprotrophs, typical to the winter grazing regime. These findings suggest that when grazers promote dominance of evergreen dwarf shrubs, they induce shifts in soil fungal communities that increase soil carbon sequestration in the long term. Thus, to predict climate\uffe2\uff80\uff90driven changes in soil carbon, grazer\uffe2\uff80\uff90induced shifts in vegetation and soil fungal communities need to be accounted for.Fungi are major drivers of ecosystem functioning. Increases in aridity are known to negatively impact fungal community composition in dryland ecosystems globally; yet, much less is known on the potential influence of other environmental drivers, and whether these relationships are linear or nonlinear.

Time period

2017\uffe2\uff80\uff932021.

Location

Global.

Major taxa studied

Fungi.

Methods

We re\uffe2\uff80\uff90analysed multiple datasets from different dryland biogeographical regions, for a total of 912 samples and 1,483 taxa. We examined geographical patterns in community diversity and composition, and spatial, edaphic and climatic factors driving them.

Results

UV index, climate seasonality, and sand content were the most important environmental predictors of community shifts, showing the strongest association with the richness of putative plant pathogens and saprobes. Important nonlinear relationships existed with each of these fungal guilds, with increases in UV and temperature seasonality above 7.5 and 900 SD (standard deviation x 100 of the mean monthly temperature), respectively, being associated with an increased probability of plant pathogen and unspecified saprotroph occurrence. Conversely, these environmental parameters had a negative relationship with litter and soil saprotroph richness. Consequently, these ecological groups might be particularly sensitive to shifts in UV radiation and climate seasonality, which is likely to disturb current plant\uffe2\uff80\uff93soil dynamics in drylands.

Main conclusions

Our synthesis integrates fungal community data from drylands across the globe, allowing the investigation of fungal distribution and providing the first evidence of shifts in fungal diversity and composition of key fungal ecological groups along diverse spatial, climatic and edaphic gradients in these widely distributed ecosystems. Our findings imply that shifts in soil structure and seasonal climatic patterns induced by global change will have disproportionate consequences for the distribution of fungal groups linked to vegetation and biogeochemical cycling in drylands, with implications for plant\uffe2\uff80\uff93soil interactions in drylands.The climate\uffe2\uff80\uff90driven encroachment of shrubs into the Arctic is accompanied by shifts in soil fungal communities that could contribute to a net release of carbon from tundra soils. At the same time, arctic grazers are known to prevent the establishment of deciduous shrubs and, under certain conditions, promote the dominance of evergreen shrubs. As these different vegetation types associate with contrasting fungal communities, the belowground consequences of climate change could vary among grazing regimes. Yet, at present, the impact of grazing on soil fungal communities and their links to soil carbon have remained speculative. Here we tested how soil fungal community composition, diversity and function depend on tree vicinity and long\uffe2\uff80\uff90term reindeer grazing regime and assessed how the fungal communities relate to organic soil carbon stocks in an alpine treeline ecotone in Northern Scandinavia. We determined soil carbon stocks and characterized soil fungal communities directly underneath and >3\uffc2\uffa0m away from mountain birches (Betula pubescens ssp. czerepanovii) in two adjacent 55\uffe2\uff80\uff90year\uffe2\uff80\uff90old grazing regimes with or without summer grazing by reindeer (Rangifer tarandus). We show that the area exposed to year\uffe2\uff80\uff90round grazing dominated by evergreen dwarf shrubs had higher soil C:N ratio, higher fungal abundance and lower fungal diversity compared with the area with only winter grazing and higher abundance of mountain birch. Although soil carbon stocks did not differ between the grazing regimes, stocks were positively associated with root\uffe2\uff80\uff90associated ascomycetes, typical to the year\uffe2\uff80\uff90round grazing regime, and negatively associated with free\uffe2\uff80\uff90living saprotrophs, typical to the winter grazing regime. These findings suggest that when grazers promote dominance of evergreen dwarf shrubs, they induce shifts in soil fungal communities that increase soil carbon sequestration in the long term. Thus, to predict climate\uffe2\uff80\uff90driven changes in soil carbon, grazer\uffe2\uff80\uff90induced shifts in vegetation and soil fungal communities need to be accounted for.Despite decades of research, the ecological determinants of microbial diversity remain poorly understood. Here, we test two alternative hypotheses concerning the factors regulating fungal diversity in soil. The first states that higher levels of plant detritus production increase the supply of limiting resources (i.e. organic substrates) thereby increasing fungal diversity. Alternatively, greater plant diversity increases the range of organic substrates entering soil, thereby increasing the number of niches to be filled by a greater array of heterotrophic fungi. These two hypotheses were simultaneously examined in experimental plant communities consisting of one to 16 species that have been maintained for a decade. We used ribosomal intergenic spacer analysis (RISA), in combination with cloning and sequencing, to quantify fungal community composition and diversity within the experimental plant communities. We used soil microbial biomass as a temporally integrated measure of resource supply. Plant diversity was unrelated to fungal diversity, but fungal diversity was a unimodal function of resource supply. Canonical correspondence analysis (CCA) indicated that plant diversity showed a relationship to fungal community composition, although the occurrence of RISA bands and operational taxonomic units (OTUs) did not differ among the treatments. The relationship between fungal diversity and resource availability parallels similar relationships reported for grasslands, tropical forests, coral reefs, and other biotic communities, strongly suggesting that the same underlying mechanisms determine the diversity of organisms at multiple scales.

", "keywords": ["0301 basic medicine", "Plant Diversity", "0303 health sciences", "Science", "Ecology and Evolutionary Biology", "Fungi", "Biodiversity", "15. Life on land", "Plants", "Cedar Creek Natural History Area", "Fungal Diversity", "Microbial Biomass", "03 medical and health sciences", "Resource Availability", "Diversity-productivity Hypothesis", "Soil Microbiology", "Microbial Diversity"]}, "links": [{"href": "https://doi.org/10.1111/j.1461-0248.2006.00965.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20Letters", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1461-0248.2006.00965.x", "name": "item", "description": "10.1111/j.1461-0248.2006.00965.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1461-0248.2006.00965.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-09-12T00:00:00Z"}}, {"id": "10.1111/j.1574-6941.2006.00235.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:52Z", "type": "Journal Article", "created": "2006-11-21", "title": "The Effects Of Stubble Retention And Nitrogen Application On Soil Microbial Community Structure And Functional Gene Abundance Under Irrigated Maize", "description": "The effects of agronomic management practices on the soil microbial community were investigated in a maize production system in New South Wales, Australia. The site has been intensively studied to measure the impact of stubble management and N-fertilizer application on greenhouse gas emissions (CO(2) and N(2)O), N-cycling, pathology, soil structure and yield. As all of these endpoints can be regulated by microbial processes, the microbiology of the system was examined. Soil samples were taken after a winter fallow period and the diversity of the bacterial and fungal communities was measured using PCR-denaturing gradient gel electrophoresis. Stubble and N shifted the structure of bacterial and fungal communities with the primary driver being stubble addition on the fungal community structure (P<0.05 for all effects). Changes in C, N (total and NO(3)), K and Na, were correlated (P<0.05) with variation in the microbial community structure. Quantitative PCR showed that nifH (nitrogen fixation) and napA (denitrification) gene abundance increased upon stubble retention, whereas amoA gene numbers were increased by N addition. These results showed that the management of both stubble and N have significant and long-term impacts on the size and structure of the soil microbial community at phylogenetic and functional levels.", "keywords": ["Electrophoresis", "0301 basic medicine", "Nitrogen", "Genes", " Fungal", "Polymerase Chain Reaction", "Zea mays", "630", "Soil", "03 medical and health sciences", "Nitrogen Fixation", "Cluster Analysis", "Electrophoresis", " Gel", " Two-Dimensional", "Fertilizers", "Soil Microbiology", "2. Zero hunger", "Gel", "0303 health sciences", "Bacteria", "Bacterial", "Australia", "Fungi", "Agriculture", "15. Life on land", "6. Clean water", "Fungal", "Genes", "Genes", " Bacterial", "13. Climate action", "Two-Dimensional"]}, "links": [{"href": "https://doi.org/10.1111/j.1574-6941.2006.00235.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1574-6941.2006.00235.x", "name": "item", "description": "10.1111/j.1574-6941.2006.00235.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1574-6941.2006.00235.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-03-01T00:00:00Z"}}, {"id": "10.1111/j.1469-8137.2012.04050.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:49Z", "type": "Journal Article", "created": "2012-01-31", "title": "Direct And Indirect Influences Of 8 Yr Of Nitrogen And Phosphorus Fertilization On Glomeromycota In An Alpine Meadow Ecosystem", "description": "We measured the influences of soil fertility and plant community composition on Glomeromycota, and tested the prediction of the functional equilibrium hypothesis that increased availability of soil resources will reduce the abundance of arbuscular mycorrhizal (AM) fungi. Communities of plants and AM fungi were measured in mixed roots and in Elymus nutans roots across an experimental fertilization gradient in an alpine meadow on the Tibetan Plateau. As predicted, fertilization reduced the abundance of Glomeromycota as well as the species richness of plants and AM fungi. The response of the glomeromycotan community was strongly linked to the plant community shift towards dominance by Elymus nutans. A reduction in the extraradical hyphae of AM fungi was associated with both the changes in soil factors and shifts in the plant community composition that were caused by fertilization. Our findings highlight the importance of soil fertility in regulating both plant and glomeromycotan communities, and emphasize that high fertilizer inputs can reduce the biodiversity of plants and AM fungi, and influence the sustainability of ecosystems.", "keywords": ["2. Zero hunger", "Elymus", "Nitrogen", "Hyphae", "Phosphorus", "Biodiversity", "04 agricultural and veterinary sciences", "Spores", " Fungal", "15. Life on land", "Tibet", "Soil", "Species Specificity", "13. Climate action", "Mycorrhizae", "Linear Models", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Fertilizers", "Glomeromycota", "Ecosystem"]}, "links": [{"href": "https://doi.org/10.1111/j.1469-8137.2012.04050.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1469-8137.2012.04050.x", "name": "item", "description": "10.1111/j.1469-8137.2012.04050.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1469-8137.2012.04050.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-01-31T00:00:00Z"}}, {"id": "10.1111/j.1469-8137.2011.03776.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:49Z", "type": "Journal Article", "created": "2011-06-08", "title": "Seven Years Of Carbon Dioxide Enrichment, Nitrogen Fertilization And Plant Diversity Influence Arbuscular Mycorrhizal Fungi In A Grassland Ecosystem", "description": "\u2022 We tested the prediction that the abundance and diversity of arbuscular mycorrhizal (AM) fungi are influenced by resource availability and plant community composition by examining the joint effects of carbon dioxide (CO(2) ) enrichment, nitrogen (N) fertilization and plant diversity on AM fungi. \u2022 We quantified AM fungal spores and extramatrical hyphae in 176 plots after 7 yr of treatment with all combinations of ambient or elevated CO(2) (368 or 560 ppm), with or without N fertilization (0 or 4 g Nm(-2) ), and one (monoculture) or 16 host plant species (polyculture) in the BioCON field experiment at Cedar Creek Ecosystem Science Reserve, Minnesota, USA. \u2022 Extramatrical hyphal lengths were increased by CO(2) enrichment, whereas AM spore abundance decreased with N fertilization. Spore abundance, morphotype richness and extramatrical hyphal lengths were all greater in monoculture plots. A structural equation model showed AM fungal biovolume was most influenced by CO(2) enrichment, plant community composition and plant richness, whereas spore richness was most influenced by fungal biovolume, plant community composition and plant richness. \u2022 Arbuscular mycorrhizal fungi responded to differences in host community and resource availability, suggesting that mycorrhizal functions, such as carbon sequestration and soil stability, will be affected by global change.", "keywords": ["0106 biological sciences", "Nitrogen", "Minnesota", "Hyphae", "Poaceae", "Models", " Biological", "01 natural sciences", "nitrogen", "Soil", "Mycorrhizae", "Biomass", "Fertilizers", "Ecosystem", "Soil Microbiology", "vesicular-arbuscular mycorrhizas", "580", "2. Zero hunger", "grassland ecology", "grasslands", "carbon dioxide", "Biodiversity", "04 agricultural and veterinary sciences", "Carbon Dioxide", "Spores", " Fungal", "15. Life on land", "plant diversity", "0401 agriculture", " forestry", " and fisheries", "Plant Shoots"]}, "links": [{"href": "https://doi.org/10.1111/j.1469-8137.2011.03776.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1469-8137.2011.03776.x", "name": "item", "description": "10.1111/j.1469-8137.2011.03776.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1469-8137.2011.03776.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-06-08T00:00:00Z"}}, {"id": "10.3389/fpls.2017.01263", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:20:36Z", "type": "Journal Article", "created": "2017-07-19", "title": "Application of Mycorrhiza and Soil from a Permaculture System Improved Phosphorus Acquisition in Naranjilla", "description": "Naranjilla (Solanum quitoense) is a perennial shrub plant mainly cultivated in Ecuador, Colombia, and Central America where it represents an important cash crop. Current cultivation practices not only cause deforestation and large-scale soil degradation but also make plants highly susceptible to pests and diseases. The use of arbuscular mycorrhizal fungi (AMF) can offer a possibility to overcome these problems. AMF can act beneficially in various ways, for example by improving plant nutrition and growth, water relations, soil structure and stability and protection against biotic and abiotic stresses. In this study, the impact of AMF inoculation on growth and nutrition parameters of naranjilla has been assessed. For inoculation three European reference AMF strains (Rhizoglomus irregulare, Claroideoglomus claroideum, and Cetraspora helvetica) and soils originating from three differently managed naranjilla plantations in Ecuador (conventional, organic, and permaculture) have been used. This allowed for a comparison of the performance of exotic AMF strains (reference strains) versus native consortia contained in the three soils used as inocula. To study fungal communities present in the three soils, trap cultures have been established using naranjilla as host plant. The community structures of AMF and other fungi inhabiting the roots of trap cultured naranjilla were assessed using next generation sequencing (NGS) methods. The growth response experiment has shown that two of the three reference AMF strains, a mixture of the three and soil from a permaculture site led to significantly better acquisition of phosphorus (up to 104%) compared to uninoculated controls. These results suggest that the use of AMF strains and local soils as inoculants represent a valid approach to improve nutrient uptake efficiency of naranjilla and consequently to reduce inputs of mineral fertilizers in the cultivation process. Improved phosphorus acquisition after inoculation with permaculture soil might have been caused by a higher abundance of AMF and the presence of Piriformospora indica as revealed by NGS. A higher frequency of AMF and enhanced root colonization rates in the trap cultures supplemented with permaculture soil highlight the importance of diverse agricultural systems for soil quality and crop production.", "keywords": ["next generation sequencing", "0301 basic medicine", "2. Zero hunger", "permaculture", "0303 health sciences", "Piriformospora indica", "Crop health", " quality", " protection", "Plant culture", "naranjilla", "arbuscular mycorrhizal fungi", "Plant Science", "15. Life on land", "fungal communities", "SB1-1110", "Soil", "03 medical and health sciences", "naranjilla", " arbuscular mycorrhizal fungi", " fungal communities", " Piriformospora indica", " farming practices", " permaculture", " next generation sequencing", "farming practices", "Sciences exactes et naturelles"]}, "links": [{"href": "https://dipot.ulb.ac.be/dspace/bitstream/2013/333899/1/doi_317543.pdf"}, {"href": "https://doi.org/10.3389/fpls.2017.01263"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2017.01263", "name": "item", "description": "10.3389/fpls.2017.01263", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2017.01263"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-07-19T00:00:00Z"}}, {"id": "10.1111/nph.15429", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-26T16:18:56Z", "type": "Journal Article", "created": "2018-09-21", "title": "Adapt your shuttling proteins for virulence: a lesson from the corn smut fungus Ustilago maydis", "description": "

This article is a Commentary on Krombach et\uffc2\uffa0al., 220: 553\uffe2\uff80\uff93566.Feedback between plants and soil microbial communities can be a powerful driver of vegetation dynamics. Plants elicit changes in the soil microbiome that either promote or suppress conspecifics at the same location, thereby regulating population density\uffe2\uff80\uff90dependence and species co\uffe2\uff80\uff90existence. Such effects are often attributed to the accumulation of host\uffe2\uff80\uff90specific antagonistic or beneficial microbiota in the rhizosphere. However, the identity and host\uffe2\uff80\uff90specificity of the microbial taxa involved are rarely empirically assessed. Here we review the evidence for host\uffe2\uff80\uff90specificity in plant\uffe2\uff80\uff90associated microbes and propose that specific plant\uffe2\uff80\uff93soil feedbacks can also be driven by generalists. We outline the potential mechanisms by which generalist microbial pathogens, mutualists and decomposers can generate differential effects on plant hosts and synthesize existing evidence to predict these effects as a function of plant investments into defence, microbial mutualists and dispersal. Importantly, the capacity of generalist microbiota to drive plant\uffe2\uff80\uff93soil feedbacks depends not only on the traits of individual plants but also on the phylogenetic and functional diversity of plant communities. Identifying factors that promote specialization or generalism in plant\uffe2\uff80\uff93microbial interactions and thereby modulate the impact of microbiota on plant performance will advance our understanding of the mechanisms underlying plant\uffe2\uff80\uff93soil feedback and the ways it contributes to plant co\uffe2\uff80\uff90existence.

How the multiple facets of soil fungal diversity vary worldwide remains virtually unknown, hindering the management of this essential species-rich group. By sequencing high-resolution DNA markers in over 4000 topsoil samples from natural and human-altered ecosystems across all continents, we illustrate the distributions and drivers of different levels of taxonomic and phylogenetic diversity of fungi and their ecological groups. We show the impact of precipitation and temperature interactions on local fungal species richness (alpha diversity) across different climates. Our findings reveal how temperature drives fungal compositional turnover (beta diversity) and phylogenetic diversity, linking them with regional species richness (gamma diversity). We integrate fungi into the principles of global biodiversity distribution and present detailed maps for biodiversity conservation and modeling of global ecological processes.

", "keywords": ["Supplementary Data", "biodiversity", " fungi", " ecology", "QH301 Biology", "Diversity (politics)", "Plant Science", "Biodiversity conservation", "Fungal Diversity", "Agricultural and Biological Sciences", "Soil", "Life", "Sociology", "WATER", "Global biodiversity distribution", "Fungal diversity", "Phylogeny", "Soil Microbiology", "2. Zero hunger", "Multidisciplinary", "Earth", " Environmental", " Ecological", " and Space Sciences", "Geography", "Ecology", "soil fungal diversity", "4. Education", "SPECIES RICHNESS", "Life Sciences", "https://www.science.org/doi/suppl/10.1126/sciadv.adj8016/suppl_file/sciadv.adj8016_sm.pdf", "Biodiversity", "FOS: Sociology", "global biodiversity distribution", "sienet", "https://www.science.org/doi/suppl/10.1126/sciadv.adj8016/suppl_file/sciadv.adj8016_tables_s1_to_s13.zip", "Diversity and Evolution of Fungal Pathogens", "570", "Supplementary Information", "DNA markers", "QH301", "Sequencing high-resolution DNA", "Biochemistry", " Genetics and Molecular Biology", "monimuotoisuus", "Mycorrhizal Fungi and Plant Interactions", "Life Science", "Humans", "14. Life underwater", "General", "Global ecological processes", "Biology", "Ecosystem", "Ecology", " Evolution", " Behavior and Systematics", "global ecological processes", "Soil fungal diversity", "microbiology", "Fungi", "Water", "Cell Biology", "15. Life on land", "luonnon monimuotoisuus", "Agronomy", "biodiversiteetti", "LIFE", "ekosysteemit (ekologia)", "Evolution and Ecology of Endophyte-Grass Symbiosis", "13. Climate action", "Ecology", " evolutionary biology", "Earth and Environmental Sciences", "FOS: Biological sciences", "Anthropology", "ta1181", "biodiversity conservation", "Species richness"]}, "links": [{"href": "https://www.science.org/doi/epdf/10.1126/sciadv.adj8016"}, {"href": "https://www.science.org/doi/pdf/10.1126/sciadv.adj8016"}, {"href": "https://doi.org/10.1126/sciadv.adj8016"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20Advances", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1126/sciadv.adj8016", "name": "item", "description": "10.1126/sciadv.adj8016", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1126/sciadv.adj8016"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-01T00:00:00Z"}}, {"id": "10.1371/journal.pbio.3002127", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:19:19Z", "type": "Journal Article", "created": "2023-05-18", "title": "A glycan receptor kinase facilitates intracellular accommodation of arbuscular mycorrhiza and symbiotic rhizobia in the legume Lotus japonicus", "description": "

Receptors that distinguish the multitude of microbes surrounding plants in the environment enable dynamic responses to the biotic and abiotic conditions encountered. In this study, we identify and characterise a glycan receptor kinase, EPR3a, closely related to the exopolysaccharide receptor EPR3. Epr3a is up-regulated in roots colonised by arbuscular mycorrhizal (AM) fungi and is able to bind glucans with a branching pattern characteristic of surface-exposed fungal glucans. Expression studies with cellular resolution show localised activation of the Epr3a promoter in cortical root cells containing arbuscules. Fungal infection and intracellular arbuscule formation are reduced in epr3a mutants. In vitro, the EPR3a ectodomain binds cell wall glucans in affinity gel electrophoresis assays. In microscale thermophoresis (MST) assays, rhizobial exopolysaccharide binding is detected with affinities comparable to those observed for EPR3, and both EPR3a and EPR3 bind a well-defined \uffce\uffb2-1,3/\uffce\uffb2-1,6 decasaccharide derived from exopolysaccharides of endophytic and pathogenic fungi. Both EPR3a and EPR3 function in the intracellular accommodation of microbes. However, contrasting expression patterns and divergent ligand affinities result in distinct functions in AM colonisation and rhizobial infection in Lotus japonicus. The presence of Epr3a and Epr3 genes in both eudicot and monocot plant genomes suggest a conserved function of these receptor kinases in glycan perception. Fungal communities play a major role as decomposers in the Earth's ecosystems. Their community-level responses to elevated CO 2 (eCO 2 ), one of the major global change factors impacting ecosystems, are not well understood. Using 28S rRNA gene amplicon sequencing and co-occurrence ecological network approaches, we analyzed the response of soil fungal communities in the BioCON (biodiversity, CO 2 , and N deposition) experimental site in Minnesota, USA, in which a grassland ecosystem has been exposed to eCO 2 for 12 years. Long-term eCO 2 did not significantly change the overall fungal community structure and species richness, but significantly increased community evenness and diversity. The relative abundances of 119 operational taxonomic units (OTU; \uffe2\uff88\uffbc27% of the total captured sequences) were changed significantly. Significantly changed OTU under eCO 2 were associated with decreased overall relative abundance of Ascomycota, but increased relative abundance of Basidiomycota. Co-occurrence ecological network analysis indicated that eCO 2 increased fungal community network complexity, as evidenced by higher intermodular and intramodular connectivity and shorter geodesic distance. In contrast, decreased connections for dominant fungal species were observed in the eCO 2 network. Community reassembly of unrelated fungal species into highly connected dense modules was observed. Such changes in the co-occurrence network topology were significantly associated with altered soil and plant properties under eCO 2 , especially with increased plant biomass and NH 4 + availability. This study provided novel insights into how eCO 2 shapes soil fungal communities in grassland ecosystems.

", "keywords": ["580", "0301 basic medicine", "0303 health sciences", "electric network topology", "Minnesota", "Molecular Sequence Data", "Fungi", "carbon dioxide", "Sequence Analysis", " DNA", "Carbon Dioxide", "15. Life on land", "Biota", "DNA", " Ribosomal", "333", "03 medical and health sciences", "13. Climate action", "XXXXXX - Unknown", "RNA", " Ribosomal", " 28S", "11. Sustainability", "fungi", "ecology", "DNA", " Fungal", "Soil Microbiology", "biodiversity"]}, "links": [{"href": "https://doi.org/10.1128/aem.04040-14"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20and%20Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/aem.04040-14", "name": "item", "description": "10.1128/aem.04040-14", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/aem.04040-14"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-04-01T00:00:00Z"}}, {"id": "10.1128/msystems.00344-21", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:19:03Z", "type": "Journal Article", "created": "2021-05-10", "title": "Network Properties of Local Fungal Communities Reveal the Anthropogenic Disturbance Consequences of Farming Practices in Vineyard Soils", "description": "

Soil fungal communities play a key role in agroecosystem sustainability. The complexity of fungal communities, at both taxonomic and functional levels, makes it difficult to find clear patterns connecting community composition with ecosystem function and to understand the impact of biotic (interspecies interactions) and abiotic (e.g., climate or anthropogenic disturbances) factors on it.

", "keywords": ["Ecolog\u00eda (Biolog\u00eda)", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "agroecosystems", "local networks", "Local networks", "Microbiolog\u00eda (Biolog\u00eda)", "579", "Ecolog\u00eda", "Emergent properties", "15. Life on land", "Microbiolog\u00eda", "fungal communities", "Microbiology", "574", "QR1-502", "Fungal communities", "03 medical and health sciences", "2401.06 Ecolog\u00eda animal", "emergent properties", "11. Sustainability", "2414 Microbiolog\u00eda", "Agroecosystems", "Research Article"]}, "links": [{"href": "https://journals.asm.org/doi/pdf/10.1128/mSystems.00344-21"}, {"href": "https://doi.org/10.1128/msystems.00344-21"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mSystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/msystems.00344-21", "name": "item", "description": "10.1128/msystems.00344-21", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/msystems.00344-21"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-29T00:00:00Z"}}, {"id": "10.1146/annurev-phyto-082718-100139", "type": "Feature", "geometry": null, "properties": {"license": "unspecified", "updated": "2026-07-26T16:19:08Z", "type": "Journal Article", "created": "2019-07-24", "title": "Molecular Interactions Between Smut Fungi and Their Host Plants", "description": "

Smut fungi are a large group of biotrophic plant pathogens that infect mostly monocot species, including economically relevant cereal crops. For years, Ustilago maydis has stood out as the model system to study the genetics and cell biology of smut fungi as well as the pathogenic development of biotrophic plant pathogens. The identification and functional characterization of secreted effectors and their role in virulence have particularly been driven forward using the U. maydis\uffe2\uff80\uff93maize pathosystem. Today, advancing tools for additional smut fungi such as Ustilago hordei and Sporisorium reilianum, as well as an increasing number of available genome sequences, provide excellent opportunities to investigate in parallel the effector function and evolution associated with different lifestyles and host specificities. In addition, genome analyses revealed similarities in the genomic signature between pathogenic smuts and epiphytic Pseudozyma species. This review elaborates on how knowledge about fungal lifestyles, genome biology, and functional effector biology has helped in understanding the biology of this important group of fungal pathogens. We highlight the contribution of the U. maydis model system but also discuss the differences from other smut fungi, which raises the importance of comparative genomic and genetic analyses in future research.

", "keywords": ["Fungal Proteins", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Fungi", "Ustilago", "Ustilaginales", "Zea mays", "Plant Diseases"]}, "links": [{"href": "https://www.annualreviews.org/doi/pdf/10.1146/annurev-phyto-082718-100139"}, {"href": "https://doi.org/10.1146/annurev-phyto-082718-100139"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Annual%20Review%20of%20Phytopathology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1146/annurev-phyto-082718-100139", "name": "item", "description": "10.1146/annurev-phyto-082718-100139", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1146/annurev-phyto-082718-100139"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-08-25T00:00:00Z"}}, {"id": "10.1155/2018/9264259", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:19:08Z", "type": "Journal Article", "created": "2018-09-06", "title": "Effects of Aged Oil Sludge on Soil Physicochemical Properties and Fungal Diversity Revealed by High-Throughput Sequencing Analysis", "description": "

The oilfield soil was contaminated for years by large quantities of aged oil sludge generated in the petroleum industry. In this study, physicochemical properties, contents of main pollutants, and fungal diversity of the aged oil sludge-contaminated soil were analyzed. Results revealed that aged oil sludge significantly changed physical and chemical properties of the receiving soil and increased the contents of main pollutants (petroleum hydrocarbons and heavy metals) in soil. Meanwhile, the internal transcribed spacer (ITS) sequencing by Illumina Miseq platform at each taxonomic level demonstrated that the toxicological effect of oil pollutants obviously influenced the fungal diversity and community structure in soil. Moreover, it was found that the presence of three genera (Cephalotheca, Lecanicillium, and Septoriella) appeared in aged oil sludge-contaminated soil. And oil pollutants promoted the growth of certain genera in Ascomycota (70.83%) and Basidiomycota (10.78%), such as Venturia, Alternaria, and Piloderma. Nevertheless, the growth of Mortierella (9.16%), Emericella (6.02%), and Bjerkandera (0.00%) was intensively limited. This study would aid thorough understanding of microbial diversity in oil-contaminated soil and thus provide new point of view to soil bioremediation.Alzheimer&rsquo;s disease (AD) is a chronic neurodegenerative disease associated with the overproduction and accumulation of amyloid-&beta; peptide and hyperphosphorylation of tau proteins in the brain. Despite extensive research on the amyloid-based mechanism of AD pathogenesis, the underlying cause of AD remains poorly understood. No disease-modifying therapies currently exist, and numerous clinical trials have failed to demonstrate any benefits. The recent discovery that the amyloid-&beta; peptide has antimicrobial activities supports the possibility of an infectious aetiology of AD and suggests that amyloid-&beta; plaque formation might be induced by infection. AD patients have a weakened blood-brain barrier and immune system and are thus at elevated risk of microbial infections. Such infections can cause chronic neuroinflammation, production of the antimicrobial amyloid-&beta; peptide, and neurodegeneration. Various pathogens, including viruses, bacteria, fungi, and parasites have been associated with AD. Most research in this area has focused on individual pathogens, with herpesviruses and periodontal bacteria being most frequently implicated. The purpose of this review is to highlight the potential role of multi-pathogen infections in AD. Recognition of the potential coexistence of multiple pathogens and biofilms in AD's aetiology may stimulate the development of novel approaches to its diagnosis and treatment. Multiple diagnostic tests could be applied simultaneously to detect major pathogens, followed by anti-microbial treatment using antiviral, antibacterial, antifungal, and anti-biofilm agents.

", "keywords": ["0301 basic medicine", "Bacteria", "Review", "Antifungal", "Microbiology", "Antiviral Agents", "QR1-502", "3. Good health", "Antibacterial", "Anti-biofilm", "03 medical and health sciences", "0302 clinical medicine", "Anti-Infective Agents", "Alzheimer Disease", "Biofilms", "Animals", "Humans", "Antiviral", "Alzheimer\u2019s disease"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1186/s12934-021-01520-7.pdf"}, {"href": "https://doi.org/10.1186/s12934-021-01520-7"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Cell%20Factories", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s12934-021-01520-7", "name": "item", "description": "10.1186/s12934-021-01520-7", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s12934-021-01520-7"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-11T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=FUNGAL&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=FUNGAL&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=FUNGAL&", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=FUNGAL&offset=50", "hreflang": "en-US"}], "numberMatched": 122, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-07-27T02:12:57.666869Z"}