{"type": "FeatureCollection", "features": [{"id": "10.1007/s00442-005-0249-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:14:49Z", "type": "Journal Article", "created": "2005-10-04", "title": "Fungal Community Composition And Metabolism Under Elevated Co2 And O-3", "description": "Atmospheric CO(2) and O(3) concentrations are increasing due to human activity and both trace gases have the potential to alter C cycling in forest ecosystems. Because soil microorganisms depend on plant litter as a source of energy for metabolism, changes in the amount or the biochemistry of plant litter produced under elevated CO(2) and O(3) could alter microbial community function and composition. Previously, we have observed that elevated CO(2) increased the microbial metabolism of cellulose and chitin, whereas elevated O(3) dampened this response. We hypothesized that this change in metabolism under CO(2) and O(3) enrichment would be accompanied by a concomitant change in fungal community composition. We tested our hypothesis at the free-air CO(2) and O(3) enrichment (FACE) experiment at Rhinelander, Wisconsin, in which Populus tremuloides, Betula papyrifera, and Acer saccharum were grown under factorial CO(2) and O(3) treatments. We employed extracellular enzyme analysis to assay microbial metabolism, phospholipid fatty acid (PLFA) analysis to determine changes in microbial community composition, and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to analyze the fungal community composition. The activities of 1,4-beta-glucosidase (+37%) and 1,4,-beta-N-acetylglucosaminidase (+84%) were significantly increased under elevated CO(2), whereas 1,4-beta-glucosidase activity (-25%) was significantly suppressed by elevated O(3). There was no significant main effect of elevated CO(2) or O(3) on fungal relative abundance, as measured by PLFA. We identified 39 fungal taxonomic units from soil using DGGE, and found that O(3) enrichment significantly altered fungal community composition. We conclude that fungal metabolism is altered under elevated CO(2) and O(3), and that there was a concomitant change in fungal community composition under elevated O(3). Thus, changes in plant inputs to soil under elevated CO(2) and O(3) can propagate through the microbial food web to alter the cycling of C in soil.", "keywords": ["0106 biological sciences", "Food Chain", "Extracellular Enzymes", "Science", "Ecology and Evolutionary Biology", "Polymerase Chain Reaction\u2013Denaturing Gradient Gel Electrophoresis", "Polymerase Chain Reaction", "01 natural sciences", "Soil Microbial Community", "Soil", "Ozone", "Health Sciences", "Acetylglucosaminidase", "Cellular and Developmental Biology", "Ecosystem", "Soil Microbiology", "beta-Glucosidase", "Fatty Acids", "Fungi", "Natural Resources and Environment", "Molecular", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Fungal Metabolism", "Carbon", "Free-air CO 2 and O 3 Enrichment", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Extracellular Space"]}, "links": [{"href": "https://doi.org/10.1007/s00442-005-0249-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-005-0249-3", "name": "item", "description": "10.1007/s00442-005-0249-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-005-0249-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-10-05T00:00:00Z"}}, {"id": "10.1007/s00248-007-9295-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:14:41Z", "type": "Journal Article", "created": "2007-08-31", "title": "Plfa Profiling Of Microbial Community Structure And Seasonal Shifts In Soils Of A Douglas-Fir Chronosequence", "description": "The impact and frequency of forest harvesting could significantly affect soil microbial community (SMC) structure and functioning. The ability of soil microorganisms to perform biogeochemical processes is critical for sustaining forest productivity and has a direct impact on decomposition dynamics and carbon storage potential. The Wind River Canopy Crane Research Forest in SW, WA, provided a unique opportunity to study a forest chronosequence and the residual effects of harvesting on the SMC in comparison to old-growth forests. The objective of this study was to determine the effect of clear-cutting and stand age on temporal dynamics of SMC and physiological stress markers using phospholipid fatty acid (PLFA) profiling. Soil microbial PLFA profiles were determined seven times over 22 months (Nov. 02 to Sep. 04) in old-growth coniferous forest stands (300-500 years) and 8 (CC8)- or 25 (CC25)-year-old replanted clear-cuts. PLFA patterns of the SMC shifted because of clear-cutting, but seasonal temporal changes had greater shifts than differences among stand age. The microbial biomass (total PLFA) and bacterial, fungal, and selected other PLFAs were significantly reduced in CC8 but not in CC25 sites relative to the old-growth sites. An increase in stress indicators [PLFA ratios of saturated/monsaturated and (cy17:0 + cy19:0)/(16:1omega7 + 18:1omega7)] in late summer was related to water stress. Although the canopy and litter input are quite different for a 25-year clear-cut compared to virgin old-growth forest, we conclude that the composition of the microbial communities, 25 years after clear-cutting, has recovered sufficiently to be much more similar to old-growth forests than a recent clear-cut at this Pacific Northwest forest site. The study shows the potential of PLFA analysis for profiling microbial communities and their stress status under field conditions, but wide temporal shifts emphasize the need for sampling over seasons to fully interpret ecosystem management impacts on microbial populations.", "keywords": ["Washington", "Time Factors", "Bacteria", "Fatty Acids", "Fungi", "Temperature", "Eukaryota", "Water", "04 agricultural and veterinary sciences", "15. Life on land", "Pseudotsuga", "Trees", "Soil", "13. Climate action", "Animals", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Seasons", "Phospholipids", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-007-9295-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-007-9295-1", "name": "item", "description": "10.1007/s00248-007-9295-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-007-9295-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-08-31T00:00:00Z"}}, {"id": "10.1007/s00248-007-9308-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:14:41Z", "type": "Journal Article", "created": "2007-09-01", "title": "Long-Term Effect Of Municipal Solid Waste Amendment On Microbial Abundance And Humus-Associated Enzyme Activities Under Semiarid Conditions", "description": "Microbial ecology is the key to understanding the function of soil biota for organic matter cycling after a single amendment of organic waste in semiarid soils. Therefore, in this paper, the long-term effect (17 years) of adding different doses of a solid municipal waste to an arid soil on humus-enzyme complexes, a very stable and long-lasting fraction of soil enzymes, as well as on microbial and plant abundance, was studied. Humic substances were extracted by 0.1 M pH 7 sodium pyrophosphate from soil samples collected in experimental plots amended with different doses of a solid municipal waste (0, 65, 130, 195, and 260 t/ha) 17 years before. The activity of different hydrolases related with the C (beta-glucosidase), N (urease), and P (alkaline phosphatase) cycles and with the formation of humic substances (o-diphenol oxidase) were determined in this extract. The density and diversity of plant cover in the plots, as well as the fungal and bacterial biomass (by analyzing phopholipid fatty acids) were also determined. In general, the amended plots showed greater humic substance-related enzymatic activity than the unamended plots. This activity increased with the dose but only up to a certain level, above which it leveled off or even diminished. Plant diversity and cover density followed the same trend. Fungal and bacterial biomass also benefited in a dose-dependent manner. Different signature molecules representing gram+ and gram- bacteria, and those corresponding to monounsaturated and saturated fatty acids showed a similar behavior. The results demonstrate that organic amendment had a noticeable long-term effect on the vegetal development, humic substances-related enzyme activity and on the development of bacteria and fungi in semiarid conditions.", "keywords": ["2. Zero hunger", "Analysis of Variance", "Time Factors", "Bacteria", "Fatty Acids", "Fungi", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "6. Clean water", "Enzymes", "Refuse Disposal", "12. Responsible consumption", "Soil", "Ergosterol", "11. Sustainability", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Humic Substances", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s00248-007-9308-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-007-9308-0", "name": "item", "description": "10.1007/s00248-007-9308-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-007-9308-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-09-02T00:00:00Z"}}, {"id": "10.1007/s13762-019-02264-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:15:29Z", "type": "Journal Article", "created": "2019-02-12", "title": "High-solids anaerobic digestion requires a trade-off between total solids, inoculum-to-substrate ratio and ammonia inhibition", "description": "Increasing total solids in anaerobic digestion can reduce the methane yield by highly complex bio-physical\u2013chemical mechanisms. Therefore, understanding those mechanisms and their main drivers becomes crucial to optimize this waste treatment biotechnology. In this study, seven batch experiments were conducted to investigate the effects of increasing the initial total solids in high-solids anaerobic digestion of the organic fraction of municipal solid waste. With inoculum-to-substrate ratio\u2009=\u20091.5 g VS/g VS and maximum total solids \u2264\u200919.6%, mono-digestion of the organic fraction of municipal solid waste showed a methane yield\u2009=\u2009174\u2013236 NmL CH4/g VS. With inoculum-to-substrate ratio \u2264\u20091.0 g VS/g VS and maximum total solids \u2265\u200924.0%, mono-digestion experiments acidified. Co-digestion of the organic fraction of municipal solid waste and beech sawdust permitted to reduce the inoculum-to-substrate ratio to 0.16 g VS/g VS while increasing total solids up to 30.2%, though achieving a lower methane yield (117\u2013156 NmL CH4/g VS). At each inoculum-to-substrate ratio, higher total solids corresponded to higher ammonia and volatile fatty acid accumulation. Thus, a 40% lower methane yield for mono-digestion was observed at a NH3 concentration \u2265\u20092.3 g N\u2013NH3/kg reactor content and total solids\u2009=\u200915.0%. Meanwhile, co-digestion lowered the nitrogen content, being the risk of acidification exacerbated only at total solids \u2265\u200920.0%. Therefore, the biodegradability of the substrate, as well as the operational total solids and inoculum-to-substrate ratio, are closely interrelated parameters determining the success of methanogenesis, but also the risk of ammonia inhibition in high-solids anaerobic digestion.", "keywords": ["Environmental Engineering", "[SDE.IE]Environmental Sciences/Environmental Engineering", "Organic fraction of municipal solid waste", "0211 other engineering and technologies", "500", "High-solids anaerobic digestion", "02 engineering and technology", "Co-digestion", "01 natural sciences", "7. Clean energy", "6. Clean water", "12. Responsible consumption", "Agricultural and Biological Sciences (all)", "Thermophilic", "13. Climate action", "Batch experiments", "11. Sustainability", "Environmental Chemistry", "Volatile fatty acids", "[SDE.IE] Environmental Sciences/Environmental Engineering", "Batch experiments; Co-digestion; High-solids anaerobic digestion; Methane yield; Organic fraction of municipal solid waste; Thermophilic; Volatile fatty acids; Environmental Engineering; Environmental Chemistry; Agricultural and Biological Sciences (all)", "Methane yield", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/743139/1/Batch%20Manuscript%20last%20for%20IRIS.pdf"}, {"href": "http://link.springer.com/content/pdf/10.1007/s13762-019-02264-z.pdf"}, {"href": "https://hal.inrae.fr/hal-02961893/file/Batch%20Manuscript_revf.pdf"}, {"href": "https://doi.org/10.1007/s13762-019-02264-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Environmental%20Science%20and%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s13762-019-02264-z", "name": "item", "description": "10.1007/s13762-019-02264-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s13762-019-02264-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-12T00:00:00Z"}}, {"id": "10.1007/s00442-006-0562-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:14:49Z", "type": "Journal Article", "created": "2006-10-10", "title": "Is Microbial Community Composition In Boreal Forest Soils Determined By Ph, C-To-N Ratio, The Trees, Or All Three?", "description": "In Fennoscandian boreal forests, soil pH and N supply generally increase downhill as a result of water transport of base cations and N, respectively. Simultaneously, forest productivity increases, the understory changes from ericaceous dwarf shrubs to tall herbs; in the soil, fungi decrease whereas bacteria increase. The composition of the soil microbial community is mainly thought to be controlled by the pH and C-to-N ratio of the substrate. However, the latter also determines the N supply to plants, the plant community composition, and should also affect plant allocation of C below ground to roots and a major functional group of microbes, mycorrhizal fungi. We used phospholipid fatty acids (PLFAs) to analyze the potential importance of mycorrhizal fungi by comparing the microbial community composition in a tree-girdling experiment, where tree belowground C allocation was terminated, and in a long-term (34 years) N loading experiment, with the shifts across a natural pH and N supply gradient. Both tree girdling and N loading caused a decline of ca. 45% of the fungal biomarker PLFA 18:2omega6,9, suggesting a common mechanism, i.e., that N loading caused a decrease in the C supply to ectomycorrhizal fungi just as tree girdling did. The total abundance of bacterial PLFAs did not respond to tree girdling or to N loading, in which cases the pH (of the mor layer) did not change appreciably, but bacterial PLFAs increased considerably when pH increased across the natural gradient. Fungal biomass was high only in acid soil (pH < 4.1) with a high C-to-N ratio (>38). According to a principal component analysis, the soil C-to-N ratio was as good as predictor of microbial community structure as pH. Our study thus indicated the soil C-to-N ratio, and the response of trees to this ratio, as important factors that together with soil pH influence soil microbial community composition.", "keywords": ["Sweden", "Soil", "Bacteria", "Nitrogen", "Mycorrhizae", "Fatty Acids", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "15. Life on land", "Carbon", "Soil Microbiology", "Trees"]}, "links": [{"href": "https://doi.org/10.1007/s00442-006-0562-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Oecologia", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00442-006-0562-5", "name": "item", "description": "10.1007/s00442-006-0562-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00442-006-0562-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-10-11T00:00:00Z"}}, {"id": "10.1007/s11104-012-1547-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:15:16Z", "type": "Journal Article", "created": "2012-12-14", "title": "Snow Cover Manipulation Effects On Microbial Community Structure And Soil Chemistry In A Mountain Bog", "description": "Background and Aims Alterations in snow cover driven by climate change may impact ecosystem functioning, including biogeochemistry and soil (microbial) processes. We elucidated the effects of snow cover manipulation (SCM) on above-and belowground processes in a temperate peatland.", "keywords": ["trends", "2. Zero hunger", "570", "biomass", "tundra soils", "variability", "[SDE.MCG]Environmental Sciences/Global Changes", "dynamics", "04 agricultural and veterinary sciences", "15. Life on land", "forest soil", "freeze-thaw cycles", "Microbial communities; peatland; phosphatase activity; Phospholipid fatty acids (PLFA); Snow cover manipulation; \uf020Winter Ecology", "01 natural sciences", "nitrogen", "13. Climate action", "[SDE]Environmental Sciences", "climate-change", "rv-coefficient", "0401 agriculture", " forestry", " and fisheries", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://eprints.soton.ac.uk/412453/2/Robroek_2013_Plant_and_Soil.pdf"}, {"href": "https://doi.org/10.1007/s11104-012-1547-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11104-012-1547-2", "name": "item", "description": "10.1007/s11104-012-1547-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11104-012-1547-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-12-16T00:00:00Z"}}, {"id": "10.1007/s12649-020-01023-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:15:27Z", "type": "Journal Article", "created": "2020-03-26", "title": "A Multiproduct Biorefinery Approach for the Production of Hydrogen, Methane and Volatile Fatty Acids from Agricultural Waste", "description": "Abstract

A pilot scale biorefinery platform for the treatment of agro-waste and the production of hydrogen, methane and volatile fatty acids was studied in real environment. The system adopted was a two stage anaerobic process where hydrogen and volatile fatty acids were produced in the first phase (fermentation) and methane in the second one (digestion). The study demonstrated the possibility to produce a biogas composed by hydrogen and methane (10% and 55%, respectively) while recovering volatile fatty acids. The yield for acids production was equivalent to 0.13\uffc2\uffa0gVFA/gTVS (as COD) with acetate and butyrate as dominant observed species.

Graphic Abstra", "keywords": ["Horizon 2020", "Environmental Engineering", "Circular economy", "Renewable Energy", " Sustainability and the Environment", "Polyhydroxyalkanoates", "7. Clean energy", "01 natural sciences", "6. Clean water", "Biorefinery", "12. Responsible consumption", "Bio-economy", "Bioresource recovery", "Euratom", "13. Climate action", "Volatile fatty acids", "European Union (EU)", "Waste Management and Disposal", "Agricultural waste", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s12649-020-01023-3.pdf"}, {"href": "https://doi.org/10.1007/s12649-020-01023-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Waste%20and%20Biomass%20Valorization", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s12649-020-01023-3", "name": "item", "description": "10.1007/s12649-020-01023-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s12649-020-01023-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-26T00:00:00Z"}}, {"id": "10.1016/j.biortech.2018.04.095", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:15:56Z", "type": "Journal Article", "created": "2018-04-25", "title": "Performance of a biotrickling filter for the anaerobic utilization of gas-phase methanol coupled to thiosulphate reduction and resource recovery through volatile fatty acids production", "description": "The anaerobic removal of continuously fed gas-phase methanol (2.5-30\u202fg/m3.h) and the reduction of step-fed thiosulphate (1000\u202fmg/L) was investigated in a biotrickling filter (BTF) operated for 123\u202fd at an empty bed residence time (EBRT) of 4.6 and 2.3\u202fmin. The BTF performance during steady step-feed and special operational phases like intermittent liquid trickling in 6 and 24\u202fh cycles and operation without pH regulation were evaluated. Performance of the BTF was not affected and nearly 100% removal of gas-phase methanol was achieved with an ECmax of 21\u202fg/m3.h. Besides, >99% thiosulphate reduction was achieved, in all the phases of operation. The production of sulphate, H2S and volatile fatty acids (VFA) was monitored and a maximum of 2500\u202fmg/L of acetate, 200\u202fmg/L of propionate, 150\u202fmg/L of isovalerate and 100\u202fmg/L isobutyrate was produced.", "keywords": ["Biodegradation", " Environmental", "Bioreactors", "Methanol", "Thiosulfates", "0211 other engineering and technologies", "02 engineering and technology", "Fatty Acids", " Volatile", "01 natural sciences", "Filtration", "6. Clean water", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Eregowda, Tejaswini, Matanhike, Luck, Rene, Eldon R., Lens, Piet N.L.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.biortech.2018.04.095"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biortech.2018.04.095", "name": "item", "description": "10.1016/j.biortech.2018.04.095", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biortech.2018.04.095"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-09-01T00:00:00Z"}}, {"id": "10.1016/j.biortech.2018.12.064", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:15:56Z", "type": "Journal Article", "created": "2018-12-22", "title": "ADM1 based mathematical model of trace element complexation in anaerobic digestion processes", "description": "In this study, a new model based on anaerobic digestion model no.1 (ADM1) approach has been proposed to simulate trace elements (TEs) complexation, precipitation and their effect on the anaerobic batch methane production. TEs complexation reactions with VFAs and EDTA have been incorporated in an extended ADM1 model which considers TE precipitation/dissolution reactions as well as biodegradation processes. The kinetic model tracks the dynamics of 90 state variables which constitute the components of the proposed anaerobic digestion (AD) model. The incorporation of the complexation reactions required the definition of new inorganic components (EDTA species) and new complexation process rates in the ADM1 framework. The charge balance was modified accordingly to consider the effects of the additional components. The new model is able to predict: a) the effect of TE-EDTA/VFA complexation on methane production, and b) the effect of the initial calcium and magnesium concentrations on process performance.", "keywords": ["ADM1", "Trace elements", "0211 other engineering and technologies", "02 engineering and technology", "Models", " Theoretical", "Fatty Acids", " Volatile", "01 natural sciences", "7. Clean energy", "Trace Elements", "[SDU] Sciences of the Universe [physics]", "Kinetics", "Biodegradation", " Environmental", "Bioreactors", "13. Climate action", "Anaerobic digestion", "[SDU.STU] Sciences of the Universe [physics]/Earth Sciences", "Complexation", "Mathematical modeling", "Anaerobic digestion; Trace elements; Complexation; Mathematical modeling; ADM1;", "Anaerobiosis", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.biortech.2018.12.064"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biortech.2018.12.064", "name": "item", "description": "10.1016/j.biortech.2018.12.064", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biortech.2018.12.064"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-03-01T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2019.02.158", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:01Z", "type": "Journal Article", "created": "2019-02-25", "title": "Bioreduction of selenate in an anaerobic biotrickling filter using methanol as electron donor", "description": "The anaerobic bioreduction of selenate, fed in step (up to 60\u202fmg.L-1) or continuous (\u223c7\u202fmg.L-1) trickling mode, in the presence of gas-phase methanol (4.3-50\u202fg\u202fm-3.h-1) was evaluated in a biotrickling filter (BTF). During the 48\u202fd of step-feed and 41\u202fd of continuous-feed operations, average selenate removal efficiencies (RE)\u202f>\u202f90% and \u223c68% was achieved, corresponding to a selenate reduction rate of, respectively, 7.3 and 4.5\u202fmg.L-1.d-1. During the entire period of BTF operation, 65.6% of the total Se fed as SeO42- was recovered. Concerning gas-phase methanol, the maximum elimination capacity (ECmax) was 46.4\u202fg\u202fm-3.h-1, with a RE\u202f>\u202f80%. Methanol was mainly utilized for acetogenesis and converted to volatile fatty acids (VFA) in the liquid-phase. Up to 5000\u202fmg.L-1 of methanol and 800\u202fmg.L-1 of acetate accumulated in the trickling liquid of the BTF.", "keywords": ["Bacteria", "Sewage", "Methanol", "0211 other engineering and technologies", "02 engineering and technology", "Selenic Acid", "Fatty Acids", " Volatile", "Archaea", "01 natural sciences", "6. Clean water", "Biodegradation", " Environmental", "Bioreactors", "Oxidation-Reduction", "Filtration", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2019.02.158"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2019.02.158", "name": "item", "description": "10.1016/j.chemosphere.2019.02.158", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2019.02.158"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-01T00:00:00Z"}}, {"id": "10.1016/j.ijbiomac.2018.12.270", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:36Z", "type": "Journal Article", "created": "2019-01-03", "title": "Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions", "description": "Open AccessPolyhydroxyalkanoates (PHAs) production with phototrophic mixed cultures (PMCs) has been recently proposed. These cultures can be selected under the permanent presence of carbon and the PHA production can be enhanced in subsequent accumulation steps. To optimize the PHA production in accumulator reactors, this work evaluated the impact of 1) initial acetate concentration, 2) light intensity, 3) removal of residual nitrogen on the culture performance. Results indicate that low acetate concentration (<30CmM) and specific light intensities around 20W/gX are optimal operating conditions that lead to high polyhydroxybutyrate (PHB) storage yields (0.83+-0.07 Cmol-PHB/Cmol-Acet) and specific PHB production rates of 2.21+-0.07 Cmol-PHB/Cmol X d. This rate is three times higher than previously registered in non-optimized accumulation tests and enabled a PHA content increase from 15 to 30% in less than 4h. Also, it was shown for the first time, the capability of a PMC to use a real waste, fermented cheese whey, to produce PHA with a hydroxyvalerate (HV) content of 12%. These results confirm that fermented wastes can be used as substrates for PHA production with PMCs and that the energy levels in sunlight that lead to specific light intensities from 10 to 20W/gX are sufficient to drive phototrophic PHA production processes.", "keywords": ["0106 biological sciences", "1303 Biochemistry", "Light", "Nitrogen", "Polyhydroxyalkanoates (PHA)", "Fermented cheese whey (FCW)", "Phototrophic mixed cultures (PMCs)", "Acetates", "7. Clean energy", "01 natural sciences", "1315 Structural Biology", "Bioreactors", "Cheese", "Whey", "1312 Molecular Biology", "0105 earth and related environmental sciences", "Polyhydroxyalkanoates", "Volatile fatty acids (VFAs)", "Other Quantitative Biology (q-bio.OT)", "Quantitative Biology - Other Quantitative Biology", "Purple phototrophic bacteria (PPB)", "Kinetics", "Phototrophic Processes", "13. Climate action", "FOS: Biological sciences", "Fermentation"]}, "links": [{"href": "https://doi.org/10.1016/j.ijbiomac.2018.12.270"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Biological%20Macromolecules", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijbiomac.2018.12.270", "name": "item", "description": "10.1016/j.ijbiomac.2018.12.270", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijbiomac.2018.12.270"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-01T00:00:00Z"}}, {"id": "10.1111/gcb.12666", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:18:44Z", "type": "Journal Article", "created": "2014-06-21", "title": "Interactive Effects Of Elevated Co2 And Nitrogen Deposition On Fatty Acid Molecular And Isotope Composition Of Above- And Belowground Tree Biomass And Forest Soil Fractions", "description": "Abstract

Atmospheric carbon dioxide (CO2) and reactive nitrogen (N) concentrations have been increasing due to human activities and impact the global carbon (C) cycle by affecting plant photosynthesis and decomposition processes in soil. Large amounts of C are stored in plants and soils, but the mechanisms behind the stabilization of plant\uffe2\uff80\uff90 and microbial\uffe2\uff80\uff90derived organic matter (OM) in soils are still under debate and it is not clear how N deposition affects soil OM dynamics. Here, we studied the effects of 4\uffc2\uffa0years of elevated (13C\uffe2\uff80\uff90depleted) CO2 and N deposition in forest ecosystems established in open\uffe2\uff80\uff90top chambers on composition and turnover of fatty acids (FAs) in plants and soils. FAs served as biomarkers for plant\uffe2\uff80\uff90 and microbial\uffe2\uff80\uff90derived OM in soil density fractions. We analyzed above\uffe2\uff80\uff90 and belowground plant biomass of beech and spruce trees as well as soil density fractions for the total organic C and FA molecular and isotope (\uffce\uffb413C) composition. FAs did not accumulate relative to total organic C in fine mineral fractions, showing that FAs are not effectively stabilized by association with soil minerals. The \uffce\uffb413C values of FAs in plant biomass increased under high N deposition. However, the N effect was only apparent under elevated CO2 suggesting a N limitation of the system. In soil fractions, only isotope compositions of short\uffe2\uff80\uff90chain FAs (C16+18) were affected. Fractions of \uffe2\uff80\uff98new\uffe2\uff80\uff99 (experimental\uffe2\uff80\uff90derived) FAs were calculated using isotope depletion in elevated CO2 plots and decreased from free light to fine mineral fractions. \uffe2\uff80\uff98New\uffe2\uff80\uff99 FAs were higher in short\uffe2\uff80\uff90chain compared to long\uffe2\uff80\uff90chain FAs (C20\uffe2\uff88\uff9230), indicating a faster turnover of short\uffe2\uff80\uff90chain compared to long\uffe2\uff80\uff90chain FAs. Increased N deposition did not significantly affect the quantity of \uffe2\uff80\uff98new\uffe2\uff80\uff99 FAs in soil fractions, but showed a tendency of increased amounts of \uffe2\uff80\uff98old\uffe2\uff80\uff99 (pre\uffe2\uff80\uff90experimental) C suggesting that decomposition of \uffe2\uff80\uff98old\uffe2\uff80\uff99 C is retarded by high N inputs.

", "keywords": ["UFSP13-8 Global Change and Biodiversity", "2306 Global and Planetary Change", "Chemical Fractionation", "Forests", "2300 General Environmental Science", "Soil", "Fagus", "Environmental Chemistry", "Biomass", "Photosynthesis", "Picea", "General Environmental Science", "2. Zero hunger", "Global and Planetary Change", "Analysis of Variance", "Carbon Isotopes", "Ecology", "Atmosphere", "Fatty Acids", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Reactive Nitrogen Species", "13. Climate action", "2304 Environmental Chemistry", "570 Life sciences; biology", "0401 agriculture", " forestry", " and fisheries", "2303 Ecology"]}, "links": [{"href": "https://doi.org/10.1111/gcb.12666"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.12666", "name": "item", "description": "10.1111/gcb.12666", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.12666"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-07-08T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2013.03.090", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:16:53Z", "type": "Journal Article", "created": "2013-04-24", "title": "Microbial Utilisation Of Biochar-Derived Carbon", "description": "Whilst largely considered an inert material, biochar has been documented to contain a small yet significant fraction of microbially available labile organic carbon (C). Biochar addition to soil has also been reported to alter soil microbial community structure, and to both stimulate and retard the decomposition of native soil organic matter (SOM). We conducted a short-term incubation experiment using two (13)C-labelled biochars produced from wheat or eucalypt shoots, which were incorporated in an aridic arenosol to examine the fate of the labile fraction of biochar-C through the microbial community. This was achieved using compound specific isotopic analysis (CSIA) of phospholipid fatty acids (PLFAs). A proportion of the biologically-available fraction of both biochars was rapidly (within three days) utilised by gram positive bacteria. There was a sharp peak in CO2 evolution shortly after biochar addition, resulting from rapid turnover of labile C components in biochars and through positive priming of native SOM. Our results demonstrate that this CO2 evolution was at least partially microbially mediated, and that biochar application to soil can cause significant and rapid changes in the soil microbial community; likely due to addition of labile C and increases in soil pH.", "keywords": ["Carbon sequestration", "[SDE] Environmental Sciences", "Carbon Sequestration", "Chromatography", " Gas", "Magnetic Resonance Spectroscopy", "550", "short term", "[SDV]Life Sciences [q-bio]", "growth", "black carbon", "Char", "01 natural sciences", "630", "Mass Spectrometry", "c 13 plfa", "Black carbon", "soil organic matter", "Soil Pollutants", "mineralization", "Organic carbon", "Phospholipids", "Soil Microbiology", "char", "0105 earth and related environmental sciences", "2. Zero hunger", "Carbon Isotopes", "decomposition", "wheat straw", "biomass", "organic carbon", "Fatty Acids", "Western Australia", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "540", "pyrolysis", "forest soil", "carbon sequestration", "Carbon", "[SDV] Life Sciences [q-bio]", "Charcoal", "[SDE]Environmental Sciences", "0401 agriculture", " forestry", " and fisheries", "community structure", "\u00b9\u00b3C-PLFA", "Pyrolysis"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2013.03.090"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2013.03.090", "name": "item", "description": "10.1016/j.scitotenv.2013.03.090", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2013.03.090"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-11-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2014.04.029", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:17:06Z", "type": "Journal Article", "created": "2014-05-15", "title": "Biochar Suppressed The Decomposition Of Organic Carbon In A Cultivated Sandy Loam Soil: A Negative Priming Effect", "description": "Conversion of plant residues to biochar is an attractive strategy for mitigation of atmospheric carbon dioxide (CO2) emission and enhancement of carbon (C) storage in soil. However, the effect of biochar application on the decomposition of soil organic C (SOC) as well as its mechanisms is not well understood in the sandy loam soil of North China Plain. We investigated how biochar affected the decomposition of native SOC, using stable \u03b413C isotope analyses by applying biochar produced from corn straw (a C4 plant, \u03b413C\u00a0=\u00a0\u221211.9\u2030) to a sandy loam soil (\u03b413C of SOC\u00a0=\u00a0\u221224.5\u2030) under a long-term C3 crop rotation. The incubation experiment included four treatments: no amendment (Control), biochar amendment (BC, 0.5% of soil mass), inorganic nitrogen (N) amendment (IN, 100\u00a0mg\u00a0N\u00a0kg\u22121) and combined biochar and N amendments (BN). Compared with Control, N amendment significantly (P\u00a0<\u00a00.05) increased total soil CO2 emission, even when combined with biochar amendment. In contrast, biochar alone amendment did not affect total soil CO2 emission significantly. However biochar, even when combined with N amendment, significantly (P\u00a0<\u00a00.05) reduced CO2 emission from native SOC by 64.9\u201368.8%, indicating that biochar inhibited the decomposition of native SOC and the stimulation effect of inorganic N on native SOC degradation, a negative priming effect. N addition immediately stimulated the growth of microorganisms and altered microbial community structure by increasing Gram-positive bacteria compared to Control as measured by phospholipid fatty acid. Biochar amendment did not alter microbial biomass during the 720-h incubation period except at 168 and 720\u00a0h, but significantly (P\u00a0<\u00a00.05) lowered dissolved organic C (DOC) content in soil, primarily due to sorption of DOC by the biochar. Our study suggested that biochar application could effectively reduce the decomposition of native organic C and a potential effective measure for C sequestration in the test soil of the North China Plain.", "keywords": ["2. Zero hunger", "negative priming effect", "phospholipid fatty acids", "04 agricultural and veterinary sciences", "15. Life on land", "dissolved organic carbon", "01 natural sciences", "6. Clean water", "12. Responsible consumption", "adsorption", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "biochar", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2014.04.029"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2014.04.029", "name": "item", "description": "10.1016/j.soilbio.2014.04.029", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2014.04.029"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-09-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2023.109205", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:17:10Z", "type": "Journal Article", "created": "2023-10-10", "title": "Beyond PLFA: Concurrent extraction of neutral and glycolipid fatty acids provides new insights into soil microbial communities", "description": "The analysis of phospholipid fatty acids (PLFAs) is one of the most common methods used to quantify the abundance, and analyse the community structure, of soil microbes. The PLFA extraction method can yield two additional lipid fractions\u2014neutral lipids and glycolipids\u2014which potentially hold additional, valuable information on soil microbial communities. Yet its quantitative sensitivity on complete neutral lipid (NLFA) and glycolipid fatty acid (GLFA) profiles has never been validated. In this study we tested (i) if the high-throughput PLFA method can be expanded to concurrently extract complete NLFA and GLFA profiles, as well as sterols, (ii) whether taxonomic specificities of signature fatty acids are retained across the three lipid fractions in pure culture strains, and (iii) whether NLFAs and GLFAs allow soil-specific fingerprinting to the same extent as PLFA analysis. By adjusting the polarity of chloroform with 2% ethanol for solid phase extraction, pure lipid standards were fully fractionated into neutral lipids, glycolipids, and phospholipids. Sterols eluted in the neutral lipid fraction, and a betaine lipid co-eluted with phospholipids. We found consistent taxonomic specificities of fatty acid markers across the three lipid fractions by analysing pure culture extracts representative of soil microbes. Fatty acid profiles from soil extracts, however, showed stronger differences between PLFAs, NLFAs, and GLFAs than between soil types. This indicates that PLFAs and NLFAs signify different community properties (biomass vs. carbon storage, putatively), and that GLFAs are sensitive markers for community traits which behave differently than PLFAs. Although we consistently found high abundances of characteristic sterols in fungal extracts, the PLFA extraction method only yielded miniscule amounts of ergosterol from soil extracts. We argue that concomitant measurement of fatty acid profiles from all three lipid fractions is a low-effort and potentially information-rich addition to the PLFA method, and discuss its applicability for soil microbial community analyses.", "keywords": ["0301 basic medicine", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "15. Life on land", "Soil lipids", "03 medical and health sciences", "106026 \u00d6kosystemforschung", "NLFA", "Ergosterol", "Ergosterol; GLFA; NLFA; Phospholipid fatty acids; Soil lipids", "Phospholipid fatty acid", "soil lipids", "Phospholipid fatty acids", "106022 Microbiology", "GLFA", "106026 Ecosystem research"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2023.109205"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2023.109205", "name": "item", "description": "10.1016/j.soilbio.2023.109205", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2023.109205"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2023.109259", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:17:10Z", "type": "Journal Article", "created": "2023-12-01", "title": "A pulse of simulated root exudation alters the composition and temporal dynamics of microbial metabolites in its immediate vicinity", "description": "Root exudation increases the concentration of readily available carbon (C) compounds in its immediate environment. This creates \u2018hotspots\u2019 of microbial activity characterized by accelerated soil organic matter turnover with direct implications for nutrient availability for plants. However, our knowledge of the microbial metabolic processes occurring in the immediate vicinity of roots during and after a root exudation event is still limited.

Using reverse microdialysis, we simulated root exudation by releasing a13C-labelled mix of low-molecular-weight organic C compounds at mm-sized locations in undisturbed soil. Combined with stable isotope tracing, we investigated the fine-scale temporal and spatial response of microbial metabolism, soil chemistry, and traced microbial respiration and uptake of exuded compounds.

Our results show that a 9-h simulated root exudation pulse leads to i) a large local respiration event and ii) alteration of the temporal dynamics of soil metabolites over the following 12\u202fday\u202fat the exudation spot. Notably, we observed a threefold increase in ammonium concentrations at 12\u202fh and increased nitrate concentrations five days after the pulse. Moreover, various short-chain fatty acids (acetate, propionate, formate) increased over the following days, indicating altered microbial metabolic pathways and activity. Phospholipid and neutral lipid fatty acids (PLFAs, NLFAs) of all major microbial groups were significantly 13C-enriched within a 5\u202fmm radius around the microdialysis probes, but not beyond. The highest relative 13C enrichment was observed in fungal NLFAs, indicating that a significant proportion of the exuded compounds had been incorporated into fungal storage compounds.

Our findings indicate that the punctual release of low-molecular-weight organic C compounds into intact soil significantly changes microbial metabolism and activity in its immediate surroundings, enhancing mineralization of native organic nitrogen. This highlights the versatility of microbial metabolic pathways in response to rapidly changing C availability and their effectiveness in increasing nutrient availability near plant roots.", "keywords": ["Oxygen depletion", "2. Zero hunger", "570", "106022 Mikrobiologie", "Root exudation", "short-chain fatty acids", "Reverse microdialysis", "reverse microdialysis", "[SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study", "15. Life on land", "root exudation", "6. Clean water", "Short-chain fatty acids", "Sugar metabolism", "106026 \u00d6kosystemforschung", "thizosphere priming effect", "crabtree effect", "sugar metabolism", "106022 Microbiology", "[SDV.SA.SDS] Life Sciences [q-bio]/Agricultural sciences/Soil study", "106026 Ecosystem research", "Rhizosphere priming effect"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2023.109259"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2023.109259", "name": "item", "description": "10.1016/j.soilbio.2023.109259", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2023.109259"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-02-01T00:00:00Z"}}, {"id": "10.1016/j.watres.2018.06.016", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:17:24Z", "type": "Journal Article", "created": "2018-06-14", "title": "High-solids anaerobic digestion model for homogenized reactors", "description": "During high-solids anaerobic digestion (HS-AD) of the organic fraction of municipal solid waste (OFMSW), an important total solid (TS) removal occurs, leading to the modification of the reactor content mass/volume, in contrast to 'wet' anaerobic digestion (AD). Therefore, HS-AD mathematical simulations need to be approached differently than 'wet' AD simulations. This study aimed to develop a modelling tool based on the anaerobic digestion model 1 (ADM1) capable of simulating the TS and the reactor mass/volume dynamics in HS-AD of OFMSW. Four hypotheses were used, including the effects of apparent concentrations at high TS. The model simulated adequately HS-AD of OFMSW in batch and continuous mode, particularly the evolution of TS, reactor mass, ammonia and volatile fatty acids. By adequately simulating the reactor content mass/volume and the TS, the HS-AD model might bring further insight about potentially inhibitory mechanisms (i.e. NH3 buildup and/or acidification) occurring in HS-AD of OFMSW.", "keywords": ["[SDE] Environmental Sciences", "ADM1", "570", "[SDV]Life Sciences [q-bio]", "High-solids anaerobic digestion", " ADM1", " Reactor mass simulation", " Total solids", " Apparent concentrations", "0211 other engineering and technologies", "High-solids anaerobic digestion", "02 engineering and technology", "Solid Waste", "01 natural sciences", "7. Clean energy", "Reactor mass simulation", "total solids", "12. Responsible consumption", "high-solids anaerobic digestion", "Bioreactors", "Ammonia", "Apparent concentrations", "reactor mass simulation", "Anaerobiosis", "0105 earth and related environmental sciences", "apparent concentrations", "Total solids", "Models", " Theoretical", "Fatty Acids", " Volatile", "6. Clean water", "Refuse Disposal", "[SDV] Life Sciences [q-bio]", "[SDE]Environmental Sciences"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/718585/1/1-s2.0-S0043135418304603-main.pdf"}, {"href": "https://doi.org/10.1016/j.watres.2018.06.016"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.watres.2018.06.016", "name": "item", "description": "10.1016/j.watres.2018.06.016", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.watres.2018.06.016"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-10-01T00:00:00Z"}}, {"id": "10.1038/s41396-023-01527-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:17:47Z", "type": "Journal Article", "created": "2023-10-18", "title": "Community composition and physiological plasticity control microbial carbon storage across natural and experimental soil fertility gradients", "description": "Abstract

Many microorganisms synthesise carbon (C)-rich compounds under resource deprivation. Such compounds likely serve as intracellular C-storage pools that sustain the activities of microorganisms growing on stoichiometrically imbalanced substrates, making them potentially vital to the function of ecosystems on infertile soils. We examined the dynamics and drivers of three putative C-storage compounds (neutral lipid fatty acids [NLFAs], polyhydroxybutyrate [PHB], and trehalose) across a natural gradient of soil fertility in eastern Australia. Together, NLFAs, PHB, and trehalose corresponded to 8.5\uffe2\uff80\uff9340% of microbial C and 0.06\uffe2\uff80\uff930.6% of soil organic C. When scaled to \uffe2\uff80\uff9cstructural\uffe2\uff80\uff9d microbial biomass (indexed by polar lipid fatty acids; PLFAs), NLFA and PHB allocation was 2\uffe2\uff80\uff933-times greater in infertile soils derived from ironstone and sandstone than in comparatively fertile basalt- and shale-derived soils. PHB allocation was positively correlated with belowground biological phosphorus (P)-demand, while NLFA allocation was positively correlated with fungal PLFA : bacterial PLFA ratios. A complementary incubation revealed positive responses of respiration, storage, and fungal PLFAs to glucose, while bacterial PLFAs responded positively to PO43-. By comparing these results to a model of microbial C-allocation, we reason that NLFA primarily served the \uffe2\uff80\uff9creserve\uffe2\uff80\uff9d storage mode for C-limited taxa (i.e., fungi), while the variable portion of PHB likely served as \uffe2\uff80\uff9csurplus\uffe2\uff80\uff9d C-storage for P-limited bacteria. Thus, our findings reveal a convergence of community-level processes (i.e., changes in taxonomic composition that underpin reserve-mode storage dynamics) and intracellular mechanisms (e.g., physiological plasticity of surplus-mode storage) that drives strong, predictable community-level microbial C-storage dynamics across gradients of soil fertility and substrate stoichiometry.Elevated nitrogen (N) deposition may aggravate phosphorus (P) deficiency in forests in the warm humid regions of China. To our knowledge, the interactive effects of long-term N deposition and P availability on soil microorganisms in tropical replanted forests remain unclear. We conducted an N and P manipulation experiment with four treatments: control, N addition (15\uffe2\uff80\uff89g N m\uffe2\uff88\uff922\uffc2\uffb7yr\uffe2\uff88\uff921), P addition (15\uffe2\uff80\uff89g P m\uffe2\uff88\uff922\uffc2\uffb7yr\uffe2\uff88\uff921) and N and P addition (15\uffe2\uff80\uff89+\uffe2\uff80\uff8915\uffe2\uff80\uff89g N and P m\uffe2\uff88\uff922\uffc2\uffb7yr\uffe2\uff88\uff921, respectively) in disturbed (planted pine forest with recent harvests of understory vegetation and litter) and rehabilitated (planted with pine, but mixed with broadleaf returning by natural succession) forests in southern China. Nitrogen addition did not significantly affect soil microbial biomass, but significantly decreased the abundance of gram-negative bacteria PLFAs in both forest types. Microbial biomass increased significantly after P addition in the disturbed forest but not in the rehabilitated forest. No interactions between N and P additions on soil microorganisms were observed in either forest type. Our results suggest that microbial growth in replanted forests of southern China may be limited by P rather than by N and this P limitation may be greater in disturbed forests.

", "keywords": ["China", "Principal Component Analysis", "Nitrates", "Rainforest", "Nitrogen", "Microbiota", "Fatty Acids", "Forestry", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "Gram-Positive Bacteria", "Article", "Carbon Cycle", "Phosphates", "Multidisciplinary Sciences", "Soil", "Gram-Negative Bacteria", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Fertilizers", "Ecosystem", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1038/srep14378"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/srep14378", "name": "item", "description": "10.1038/srep14378", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/srep14378"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-09-23T00:00:00Z"}}, {"id": "10.1079/bjn19660035", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:18:10Z", "type": "Journal Article", "created": "2005-09-17", "title": "The Metabolism Of Oleic, Linoleic And Linolenic Acids By Sheep With Reference To Their Effects On Methane Production", "description": "

1. Nine experiments, each with one of six sheep with cannulated rumens given a constant diet of dried grass, were made in which oleic, linoleic or linolenic acid was infused into the rumen and energy and lipid metabolism were measured. One experiment was made in which palmitic acid was given. 2. Judged by changes in the composition of isolated fatty acids, the unsaturated fatty acids were hydrogenated in the rumen. An increase in the excretion of lipid in the faeces occurred when the unsaturated acids were given. The heat of combustion of the faeces increased by 12.6\uffc2\uffb13.0 kcal/100 kcal fatty acid, of which 94% was accounted for by the additional lipid. 3. Methane production fell when the unsaturated fatty acids were infused, the decreases being 13.8\uffc2\uffb11.6 kcal CH4;/I00 kcal oleic acid, 14.2\uffc2\uffb11.5 kcal CH4/100 kcal linoIeic acid and 16.4\uffc2\uffb11.3 kcal CH4/100 kcal Iinolenic acid. The introduction of a double bond into an n-alkyl acid was calculated to reduce methane production by 0.24\uffc2\uffb10.09 moles/mole double bond. 4. Because the depression of methane production on infusing the fatty acids exceeded the increase in the heat of combustion of the faeces, the metabolizable energy of the fatty acids was 104.1\uffc2\uffb15.3% of their heat of combustion. 5. The efficiencies with which the fatty acids were used to promote energy retention were 74.6\uffc2\uffb15.7% for oleic acid, 79.2\uffc2\uffb12.0 % for linoleic acid and 82.5\uffc2\uffb13.0% for linolenic acid. These efficiencies agreed with those noted in experiments by others with rats, horses and pigs given glycerides, but were higher than those noted by others when glycerides were added to the diets of ruminants.

", "keywords": ["2. Zero hunger", "Sheep", "Fatty Acids", " Essential", "Linoleic Acids", "Animals", "Oleic Acids", "Methane"], "contacts": [{"organization": "K. L. Blaxter, F. W. Wainman, J. W. Czerkawski,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1079/bjn19660035"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/British%20Journal%20of%20Nutrition", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1079/bjn19660035", "name": "item", "description": "10.1079/bjn19660035", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1079/bjn19660035"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1966-05-01T00:00:00Z"}}, {"id": "10.1079/bjn19690092", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:18:10Z", "type": "Journal Article", "created": "2005-05-19", "title": "Methane Production And Soluble Carbohydrates In The Rumen Of Sheep In Relation To The Time Of Feeding And The Effects Of Short-Term Intraruminal Infusions Of Unsaturated Fatty Acids", "description": "

1. The daily methane production of sheep given sugar-beet pulp was greater than that of sheep given hay. The rates of methane production on both diets increased during feeding and then decreased to an apparently steady value. When no food was given the rates of methane production continued to fall exponentially with a half-life of about 24 h.

2. Mixtures of unsaturated long-chain fatty acids infused into the rumen during feeding inhibited the production of methane. The inhibition was greater when the sheep were given hay than when they were given sugar-beet pulp.

3. In general the concentration of soluble carbohydrates in the rumen increased during feeding and fell rapidly to the values found before feeding as soon as the sheep finished eating. The infusion of unsaturated fatty acids during feeding did not result in an accumulation of soluble carbohydrate in the rumen.

4. It is suggested that the rapid methane production during feeding was associated with fermentation of the more soluble part of the diet and that the fermentation of carbohydrate was not inhibited by the infused fatty acids. The results are consistent with specific inhibition of methanogenesis by unsaturated fatty acids.

", "keywords": ["2. Zero hunger", "Linseed Oil", "Rumen", "Sheep", "Time Factors", "Linoleic Acids", "Depression", " Chemical", "Fatty Acids", "Carbohydrates", "Dietary Carbohydrates", "Animals", "Methane"], "contacts": [{"organization": "J. L. Clapperton, J. W. Czerkawski,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1079/bjn19690092"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/British%20Journal%20of%20Nutrition", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1079/bjn19690092", "name": "item", "description": "10.1079/bjn19690092", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1079/bjn19690092"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1969-11-01T00:00:00Z"}}, {"id": "10.1079/bjn19710051", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:18:10Z", "type": "Journal Article", "created": "2005-05-24", "title": "The Effect Of Tertiary Branched-Chain Carboxylic Acids On The Energy Metabolism Of Sheep", "description": "

1. The introduction of certain tertiary branched-chain carboxylic acids into the rumen of sheep, either by continuous infusion or by direct incorporation of the acids in the concentrate food mixture, had no effect on the oxygen consumption or carbon dioxide production of the sheep. Methane production was depressed, the extent of the depression increasing with the molecular weight of the carboxylic acid.

2. There was a small decrease in the apparent digestibility of the food and a small increase in urinary energy output.

3. There was no significant increase in the metabolizable energy intake of the sheep. There was, however, a small increase in the amount of energy stored in the body. This small increase was about 84 % of the gross energy of the branched-chain acids added.

4. It is concluded that any practical advantage to be gained from the addition of branched-chain carboxylic acids to the diet of ruminants remains unproven.

", "keywords": ["2. Zero hunger", "Sheep", "Fatty Acids", "Carboxylic Acids", "0402 animal and dairy science", "04 agricultural and veterinary sciences", "Carbon Dioxide", "Urine", "Molecular Weight", "Feces", "Oxygen Consumption", "Animals", "Animal Nutritional Physiological Phenomena", "Methane", "Stearic Acids"], "contacts": [{"organization": "J. L. Clapperton, J. W. Czerkawski,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1079/bjn19710051"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/British%20Journal%20of%20Nutrition", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1079/bjn19710051", "name": "item", "description": "10.1079/bjn19710051", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1079/bjn19710051"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1971-11-01T00:00:00Z"}}, {"id": "10.1079/bjn19780043", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:18:10Z", "type": "Journal Article", "created": "2005-05-19", "title": "Methane Production In Rumen And Lower Gut Of Sheep Given Lucerne Chaff - Effect Of Level Of Intake", "description": "

1. Methane production rates were estimated simultaneously in the rumen and caecum of sheep given 200, 400, 600, 800 and 1000 g lucerne (Medicago sativa) chaff/d using isotope dilution techniques. Estimates were also made of volatile fatty acid (VFA) production in the rumen at each level of feeding. In all studies three to four animals were used at each level of intake.

2. Production of VFA and of methane were both related to digestible energy (de) intake. Regression lines for both VFA production and methane productionv.deintake had significant intercepts indicating an input of endogenous, fermentable organic matter into the rumen in excess of 50 g/d.

3. The values obtained for rates of methane production were compared with those calculated from stoicheiometric equations relating rates of methane and VFA production. Comparisons of methane production with that predicted fromdeintake were also made.

4. Balances for digestion of food determined for the rumen indicated that the energies in the end-products were more than 100% of thedeintakes at low intakes of lucerne chaff. Correction for fermentation of apparent endogenous materials resulted in more realistic values. Endogenous materials appeared to make a significant contribution to VFA and methane production, particularly at low levels of intake.

", "keywords": ["2. Zero hunger", "Rumen", "Sheep", "Fermentation", "0402 animal and dairy science", "Animals", "Female", "04 agricultural and veterinary sciences", "Fatty Acids", " Volatile", "Methane", "Diet", "Medicago sativa"], "contacts": [{"organization": "A. M. Bryant, R. M. Murray, Ronald Leng,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1079/bjn19780043"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/British%20Journal%20of%20Nutrition", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1079/bjn19780043", "name": "item", "description": "10.1079/bjn19780043", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1079/bjn19780043"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1978-03-01T00:00:00Z"}}, {"id": "10.1080/00039420215633", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:18:11Z", "type": "Journal Article", "created": "2003-10-02", "title": "Rumen Fermentation And Nitrogen Balance Of Lambs Fed Diets Containing Plant Extracts Rich In Tannins And Saponins, And Associated Emissions Of Nitrogen And Methane", "description": "Tannins were added to experimental diets at levels of 1 and 2 g/kg DM (hydrolysable tannins; Castanea sativa wood extract) and saponins at 2 and 30 mg/kg DM (sarsaponin; Yucca schidigera extract). These levels were far below thresholds expected to be adverse in ruminants. Effects were measured in lambs by comparison with unsupplemented control diets calculated to be either deficient (10%) or adequate in protein. The diets consisted of hay, concentrate (1:1) and extra wheat starch with increasing body weight. Ruminal pH, VFA concentration, protozoa count and apparent digestibilities of organic matter and fibre did not differ among treatments. The low tannin dose significantly decreased bacteria count compared to the high saponin dose. Saponin supplementation and the high tannin dose showed some potential to reduce ruminal ammonia concentration. This was associated with weak trends towards lower urine N excretion (only tannins) and ammonia emission from manure. Methane release was increased by the low tannin dose compared to the unsupplemented control. Diet effects on heat production were not systematic. In conclusion, the extracts rich in tannins or saponins gave only slight indications for either increased body nitrogen retention or reduced nitrogen emission. However, effects might have been larger with more pronounced dietary protein deficit.", "keywords": ["Male", "2. Zero hunger", "Rumen", "Sheep", "Dose-Response Relationship", " Drug", "Nitrogen", "Plant Extracts", "0402 animal and dairy science", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "Saponins", "Fatty Acids", " Volatile", "Animal Feed", "6. Clean water", "Fermentation", "Animals", "Animal Nutritional Physiological Phenomena", "Digestion", "Dietary Proteins", "Methane", "Tannins"], "contacts": [{"organization": "Michael Kreuzer, B.J. Sliwinski, A. Machm\u00fcller, H. R. Wettstein,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1080/00039420215633"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Archiv%20f%C3%BCr%20Tierernaehrung", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1080/00039420215633", "name": "item", "description": "10.1080/00039420215633", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1080/00039420215633"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2002-12-01T00:00:00Z"}}, {"id": "10.1111/1365-2435.12329", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:18:37Z", "type": "Journal Article", "created": "2014-09-05", "title": "Interactive Effects Of C, N And P Fertilization On Soil Microbial Community Structure And Function In An Amazonian Rain Forest", "description": "Summary

Resource control over abundance, structure and functional diversity of soil microbial communities is a key determinant of soil processes and related ecosystem functioning. Copiotrophic organisms tend to be found in environments which are rich in nutrients, particularly carbon, in contrast to oligotrophs, which survive in much lower carbon concentrations.

We hypothesized that microbial biomass, activity and community structure in nutrient\uffe2\uff80\uff90poor soils of an Amazonian rain forest are limited by multiple elements in interaction. We tested this hypothesis with a fertilization experiment by adding C (as cellulose), N (as urea) and P (as phosphate) in all possible combinations to a total of 40 plots of an undisturbed tropical forest in French Guiana.

After 2\uffc2\uffa0years of fertilization, we measured a 47% higher biomass, a 21% increase in substrate\uffe2\uff80\uff90induced respiration rate and a 5\uffe2\uff80\uff90fold higher rate of decomposition of cellulose paper discs of soil microbial communities that grew in P\uffe2\uff80\uff90fertilized plots compared to plots without P fertilization. These responses were amplified with a simultaneous C fertilization suggesting P and C colimitation of soil micro\uffe2\uff80\uff90organisms at our study site.

Moreover, P fertilization modified microbial community structure (PLFAs) to a more copiotrophic bacterial community indicated by a significant decrease in the Gram\uffe2\uff80\uff90positive\uffc2\uffa0:\uffc2\uffa0Gram\uffe2\uff80\uff90negative ratio. The Fungi\uffc2\uffa0:\uffc2\uffa0Bacteria ratio increased in N fertilized plots, suggesting that fungi are relatively more limited by N than bacteria. Changes in microbial community structure did not affect rates of general processes such as glucose mineralization and cellulose paper decomposition. In contrast, community level physiological profiles under P fertilization combined with either C or N fertilization or both differed strongly from all other treatments, indicating functionally different microbial communities.

While P appears to be the most critical from the three major elements we manipulated, the strongest effects were observed in combination with either supplementary C or N addition in support of multiple element control on soil microbial functioning and community structure.

We conclude that the soil microbial community in the studied tropical rain forest and the processes it drives is finely tuned by the relative availability in C, N and P. Any shifts in the relative abundance of these key elements may affect spatial and temporal heterogeneity in microbial community structure, their associated functions and the dynamics of C and nutrients in tropical ecosystems.

", "keywords": ["tropical forest", "2. Zero hunger", "570", "phospholipid fatty acids (PLFA)", "[SDE.MCG]Environmental Sciences/Global Changes", "functional significance", "[SDV.EE.IEO] Life Sciences [q-bio]/Ecology", " environment/Symbiosis", "04 agricultural and veterinary sciences", "15. Life on land", "16. Peace & justice", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "[SDE.MCG] Environmental Sciences/Global Changes", "13. Climate action", "microbial community structure", "ecosystem functioning", "environment/Symbiosis", "[SDV.EE.ECO]Life Sciences [q-bio]/Ecology", "[SDV.EE.ECO] Life Sciences [q-bio]/Ecology", " environment/Ecosystems", "[SDV.EE.IEO]Life Sciences [q-bio]/Ecology", "0401 agriculture", " forestry", " and fisheries", "multiple resource limitation", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology", "phosphorus", "environment/Ecosystems", "soil functioning"]}, "links": [{"href": "https://doi.org/10.1111/1365-2435.12329"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Functional%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1365-2435.12329", "name": "item", "description": "10.1111/1365-2435.12329", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1365-2435.12329"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-09-29T00:00:00Z"}}, {"id": "10.1111/j.1574-6941.2010.01032.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:02Z", "type": "Journal Article", "created": "2011-01-11", "title": "Fungal And Bacterial Growth Responses To N Fertilization And Ph In The 150-Year 'Park Grass' Uk Grassland Experiment", "description": "The effects of nitrogen (N) fertilization (0-150 kg N ha\u207b\u00b9 year\u207b\u00b9 since 1865) and pH (3.3-7.4) on fungal and bacterial growth, biomass and phospholipid fatty acid (PLFA) composition were investigated in grassland soils from the 'Park Grass Experiment', Rothamsted Research, UK. Bacterial growth decreased and fungal growth increased with lower pH, resulting in a 50-fold increase in the relative importance of fungi between pH 7.4 and 3.3. The PLFA-based fungal:bacterial biomass ratio was unchanged between pH 4.5 and 7.4, and decreased only below pH 4.5. Respiration and substrate-induced respiration biomass both decreased three- to fourfold with lower pH, but biomass concentrations estimated using PLFAs were unaffected by pH. N fertilization did not affect bacterial growth and marginally affected fungal growth while PLFA biomass marker concentrations were all reduced by higher N additions. Respiration decreased with higher N application, suggesting a reduced quality of the soil organic carbon. The PLFA composition was strongly affected by both pH and N. A comparison with a pH gradient in arable soil allowed us to generalize the pH effect between systems. There are 30-50-fold increases in the relative importance of fungi between high (7.4-8.3) and low (3.3-4.5) pH with concomitant reductions of respiration by 30-70%.", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Bacteria", "Nitrogen", "Fatty Acids", "Fungi", "04 agricultural and veterinary sciences", "Carbon Dioxide", "Hydrogen-Ion Concentration", "15. Life on land", "Poaceae", "Microbiology", "United Kingdom", "03 medical and health sciences", "Soil", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Fertilizers", "Phospholipids", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1111/j.1574-6941.2010.01032.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1574-6941.2010.01032.x", "name": "item", "description": "10.1111/j.1574-6941.2010.01032.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1574-6941.2010.01032.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-01-17T00:00:00Z"}}, {"id": "10.1111/nph.12569", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:05Z", "type": "Journal Article", "created": "2013-10-31", "title": "Experimental Drought Reduces The Transfer Of Recently Fixed Plant Carbon To Soil Microbes And Alters The Bacterial Community Composition In A Mountain Meadow", "description": "Summary

Drought affects plants and soil microorganisms, but it is still not clear how it alters the carbon (C) transfer at the plant\uffe2\uff80\uff93microbial interface. Here, we tested direct and indirect effects of drought on soil microbes and microbial turnover of recent plant\uffe2\uff80\uff90derived C in a mountain meadow.

Microbial community composition was assessed using phospholipid fatty acids (PLFAs); the allocation of recent plant\uffe2\uff80\uff90derived C to microbial groups was analysed by pulse\uffe2\uff80\uff90labelling of canopy sections with 13CO2 and the subsequent tracing of the label into microbial PLFAs.

Microbial biomass was significantly higher in plots exposed to a severe experimental drought. In addition, drought induced a shift of the microbial community composition, mainly driven by an increase of Gram\uffe2\uff80\uff90positive bacteria. Drought reduced belowground C allocation, but not the transfer of recently plant\uffe2\uff80\uff90assimilated C to fungi, and in particular reduced tracer uptake by bacteria. This was accompanied by an increase of 13C in the extractable organic C pool during drought, which was even more pronounced after plots were mown.

We conclude that drought weakened the link between plant and bacterial, but not fungal, C turnover, and facilitated the growth of potentially slow\uffe2\uff80\uff90growing, drought\uffe2\uff80\uff90adapted soil microbes, such as Gram\uffe2\uff80\uff90positive bacteria.

", "keywords": ["Time Factors", "Nitrogen", "Mowing", "Mountain grassland", "Carbon Cycle", "Microbial community composition", "Soil", "Biomass", "Ecosystem", "Soil Microbiology", "2. Zero hunger", "106022 Mikrobiologie", "Carbon Isotopes", "Drought", "Research", "Microbiota", "Water", "Carbon allocation", "Microclimate", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon", "6. Clean water", "Droughts", "C pulse-labelling", "13. Climate action", "Austria", "Phospholipid fatty acids", "106022 Microbiology", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.1111/nph.12569"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.12569", "name": "item", "description": "10.1111/nph.12569", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.12569"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-10-31T00:00:00Z"}}, {"id": "10.1111/nph.14083", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:05Z", "type": "Journal Article", "created": "2016-07-11", "title": "Increased Phosphate Uptake But Not Resorption Alleviates Phosphorus Deficiency Induced By Nitrogen Deposition In Temperate Larix Principis-Rupprechtii Plantations", "description": "Summary

The imbalance between nitrogen (N) and phosphorus (P) deposition may shift temperate ecosystems from N\uffe2\uff80\uff90 to P\uffe2\uff80\uff90limitation. However, it is unclear how the imbalanced N\uffc2\uffa0:\uffc2\uffa0P input affects the strategies of plants to acquire P and, therefore, the growth of plants and the competition among species.

We conducted a 4\uffe2\uff80\uff90yr N\uffe2\uff80\uff90addition experiment in young and mature larch (Larix principis\uffe2\uff80\uff90rupprechtii) stands. Plant growth and P acquisition strategies were assessed for larch and understorey vegetation.

N addition stimulated the aboveground productivity of understorey vegetation in the young stand and larch in the mature stand, with other species unaffected. The competitive advantages of understorey vegetation in the young stand and larch in the mature stand were associated with their high stoichiometric homoeostasis. To maintain the N\uffc2\uffa0:\uffc2\uffa0P homoeostasis of these species, an increase in phosphatase activity but not P resorption efficiency increased the supply of P. Additionally, N addition accelerated P mineralization by decreasing the fungal\uffe2\uff80\uff90to\uffe2\uff80\uff90bacterial ratios and improved uptake of soil P by increasing the arbuscular mycorrhizas\uffe2\uff80\uff90to\uffe2\uff80\uff90ectomycorrhizas ratios.

Our results suggest that plants with high stoichiometric homoeostasis could better cope with N deposition\uffe2\uff80\uff90induced P\uffe2\uff80\uff90deficiency. Although P resorption efficiency showed little plasticity in response, plants activated a variety of P\uffe2\uff80\uff90acquisition pathways to alleviate the P\uffe2\uff80\uff90deficiency caused by N deposition.

", "keywords": ["2. Zero hunger", "Analysis of Variance", "Bacteria", "Nitrogen", "Acid Phosphatase", "Fatty Acids", "Fungi", "Larix", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "Plant Roots", "Phosphates", "Plant Leaves", "Species Specificity", "Mycorrhizae", "Homeostasis", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Phospholipids"]}, "links": [{"href": "https://doi.org/10.1111/nph.14083"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.14083", "name": "item", "description": "10.1111/nph.14083", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.14083"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-07-11T00:00:00Z"}}, {"id": "10.1155/2019/1751783", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:18Z", "type": "Journal Article", "created": "2019-05-02", "title": "Acetotrophic Activity Facilitates Methanogenesis from LCFA at Low Temperatures: Screening from Mesophilic Inocula", "description": "

The inoculum source plays a crucial role in the anaerobic treatment of wastewaters. Lipids are present in various wastewaters and have a high methanogenic potential, but their hydrolysis results in the production of long chain fatty acids (LCFAs) that are inhibitory to anaerobic microorganisms. Screening of inoculum for the anaerobic treatment of LCFA-containing wastewaters has been performed at mesophilic and thermophilic conditions. However, an evaluation of inocula for producing methane from LCFA-containing wastewater has not yet been conducted at low temperatures and needs to be undertaken. In this study, three inocula (one granular sludge and two municipal digester sludges) were assessed for methane production from LCFA-containing synthetic dairy wastewater (SDW) at low temperatures (10 and 20\uffc2\uffb0C). A methane yield (based on mL-CH4/g-CODadded) of 86-65% with acetate and 45-20% with SDW was achieved within 10 days using unacclimated granular sludge, whereas the municipal digester sludges produced methane only at 20\uffc2\uffb0C but not at 10\uffc2\uffb0C even after 200 days of incubation. The acetotrophic activity in the inoculum was found to be crucial for methane production from LCFA at low temperatures, highlighting the role ofMethanosaeta(acetoclastic archaea) at low temperatures. The presence of bacterial taxa from the familySyntrophaceae(Syntrophusand uncultured taxa) in the inoculum was found to be important for methane production from SDW at 10\uffc2\uffb0C. This study suggests the evaluation of acetotrophic activity and the initial microbial community characteristics by high-throughput amplicon sequencing for selecting the inoculum for producing methane at low temperatures (up to 10\uffc2\uffb0C) from lipid-containing wastewaters.

", "keywords": ["Deltaproteobacteria", "0301 basic medicine", "570", "0303 health sciences", "Sewage", "218 Environmental engineering", "Microbiota", "116 Chemical sciences", "Fatty Acids", "Temperature", "116", "Acetates", "Methanosarcinales", "6. Clean water", "03 medical and health sciences", "218", "13. Climate action", "11. Sustainability", "Anaerobiosis", "Methane", "Research Article"]}, "links": [{"href": "https://doi.org/10.1155/2019/1751783"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Archaea", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1155/2019/1751783", "name": "item", "description": "10.1155/2019/1751783", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1155/2019/1751783"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-02T00:00:00Z"}}, {"id": "10.13227/j.hjkx.2016.09.042", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:28Z", "type": "Journal Article", "description": "Nitrogen deposition increases the productivity of forest-land, yet may cause environmental damage, while soil microorganisms are sensitive to the ecological environment. The objective of this study was to investigate the effects of nitrogen deposition on soil microbial communities and the relationship among soil microbial communities, soil temperature, soil moisture and the concentration of nitrogen deposition in the Masson pine forest. The results might provide a reference for further study on the effects of nitrogen deposition on forest ecosystem of Masson pine. From May 2014 to June 2015, three nitrogen application treatments and the control treatment were set up:low nitrogen [N20,20 g\u00b7(m2\u00b7a)-1], moderate nitrogen [N40, 40 g\u00b7(m2\u00b7a)-1], high nitrogen [N60, 60 g\u00b7(m2\u00b7a)-1] and control treatment [N0, 0 g\u00b7(m2\u00b7a)-1] in the Masson pine forest. Soil microbial community structure, soil temperature and soil moisture were measured by phosphorus lipid fatty acids (PLFAs) and automated soil CO2 exchange station. The results showed that:1 Seasonal variation had a remarkable effect on soil bacteria, fungi, actinomycetes and the total PLFA(P<0.05), all kinds of soil microorganisms had the highest amounts in spring and the lowest in winter. In different seasons, the response of different soil microorganisms to nitrogen deposition was different. Generally, nitrogen deposition decreased soil microorganisms in spring and autumn, while in summer and winter, nitrogen deposition had a positive effect on soil microorganisms. 2 Nitrogen deposition had a significant effect on soil microbial community structure(P<0.05). In spring and summer, low nitrogen and moderate nitrogen decreased the soil microbial richness index and diversity index, yet increased the evenness index. In the autumn and winter, low nitrogen and moderate nitrogen promoted the soil microbial richness index, diversity index and evenness index. In all four seasons, nitrogen deposition reduced the soil microbial richness index, diversity index and evenness index. 3 Correlation analysis showed that the concentration of nitrogen deposition had a very significant negative correlation with soil bacteria (P<0.01), and a significant negative correlation with total PLFA (P<0.05). Soil temperature had a significant negative correlation with soil actinomycetes. Soil moisture had a significant positive correlation with bacteria and total PLFA. In conclusion, soil microorganisms of Masson pine forest were mainly influenced by soil moisture and less affected by soil temperature in Jinyun Mountain. Nitrogen deposition had a significant effect on microbial community structure and diversity.", "keywords": ["China", "Nitrogen", "Fatty Acids", "0211 other engineering and technologies", "Fungi", "02 engineering and technology", "Forests", "Pinus", "01 natural sciences", "Soil", "Ecosystem", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Bing-Hui He, Qing-Ping Zeng,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.13227/j.hjkx.2016.09.042"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Huan%20jing%20ke%20xue%3D%20Huanjing%20kexue", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.13227/j.hjkx.2016.09.042", "name": "item", "description": "10.13227/j.hjkx.2016.09.042", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.13227/j.hjkx.2016.09.042"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-01T00:00:00Z"}}, {"id": "10.1371/journal.pone.0038858", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:31Z", "type": "Journal Article", "created": "2012-06-11", "title": "Decline In Topsoil Microbial Quotient, Fungal Abundance And C Utilization Efficiency Of Rice Paddies Under Heavy Metal Pollution Across South China", "description": "Open AccessLos suelos agr\u00edcolas han estado cada vez m\u00e1s sujetos a la contaminaci\u00f3n por metales pesados en todo el mundo. Sin embargo, los impactos en la estructura y actividad de la comunidad microbiana del suelo de los suelos de campo a\u00fan no se han caracterizado bien. En 2009 se recolectaron muestras de tierra vegetal de campos de arroz contaminados con metales pesados (PS) y sus campos de fondo (BGS) en cuatro sitios del sur de China. Los cambios con la contaminaci\u00f3n met\u00e1lica en relaci\u00f3n con el BGS en el tama\u00f1o y la estructura de la comunidad de los microorganismos del suelo se examinaron con m\u00faltiples ensayos microbiol\u00f3gicos de medici\u00f3n de carbono de biomasa (MBC) y nitr\u00f3geno (MBN), recuento en placa de colonias cultivables y an\u00e1lisis de \u00e1cidos grasos fosfol\u00edpidos (PLFA) junto con el perfil de electroforesis en gel de gradiente desnaturalizante (DGGE) del gen de ARNr 16S y ARNr 18S y ensayo de PCR en tiempo real. Adem\u00e1s, se llev\u00f3 a cabo una incubaci\u00f3n de laboratorio de 7 d\u00edas a una temperatura constante de 25 \u00b0C para realizar un seguimiento adicional de los cambios en la actividad metab\u00f3lica. Si bien la disminuci\u00f3n de la contaminaci\u00f3n por metales en MBC y MBN, as\u00ed como en el tama\u00f1o de la poblaci\u00f3n cultivable, el contenido total de PLFA y el n\u00famero de bandas DGGE de bacterias no se observaron de manera significativa y consistente, de hecho se observ\u00f3 una reducci\u00f3n significativa de la contaminaci\u00f3n por metales en el cociente microbiano, en el tama\u00f1o de la poblaci\u00f3n f\u00fangica cultivable y en la proporci\u00f3n de PLFA f\u00fangicos a bacterianos de manera consistente en todos los sitios en una medida que var\u00eda de 6% a 74%. Adem\u00e1s, se observ\u00f3 un aumento consistentemente significativo en el cociente metab\u00f3lico de hasta un 68% bajo contaminaci\u00f3n en todos los sitios. Estas observaciones apoyaron un cambio de la comunidad microbiana con disminuci\u00f3n en su abundancia, disminuci\u00f3n en la proporci\u00f3n de hongos y, por lo tanto, en la eficiencia de utilizaci\u00f3n de C bajo contaminaci\u00f3n en los suelos. Adem\u00e1s, las proporciones de cociente microbiano, de hongos a bacterias y qCO2 son mejores indicativas de los impactos de los metales pesados en la estructura y actividad de la comunidad microbiana. Los efectos potenciales de estos cambios en el ciclo del carbono y la producci\u00f3n de CO2 en los arrozales contaminados merecen m\u00e1s estudios de campo.", "keywords": ["Microbial population biology", "Colony Count", " Microbial", "Agricultural and Biological Sciences", "Sociology", "Soil water", "Soil Pollutants", "Soil Microbiology", "2. Zero hunger", "Principal Component Analysis", "Temperature gradient gel electrophoresis", "Ecology", "Q", "Fatty Acids", "R", "Life Sciences", "Agriculture", "04 agricultural and veterinary sciences", "Biota", "Pollution", "6. Clean water", "FOS: Sociology", "Chemistry", "Physical Sciences", "Environmental chemistry", "Medicine", "Research Article", "Environmental Monitoring", "16S ribosomal RNA", "China", "Microorganism", "Environmental Impact of Heavy Metal Contamination", "Nitrogen", "Science", "Population", "Soil Science", "Real-Time Polymerase Chain Reaction", "Environmental science", "Microbial Ecology", "12. Responsible consumption", "Metals", " Heavy", "Genetics", "Biology", "Demography", "Bacteria", "Denaturing Gradient Gel Electrophoresis", "Marine Microbial Diversity and Biogeography", "Oryza", "15. Life on land", "Topsoil", "Carbon", "Agronomy", "RNA", " Ribosomal", "13. Climate action", "FOS: Biological sciences", "Environmental Science", "0401 agriculture", " forestry", " and fisheries", "Soil Carbon Dynamics and Nutrient Cycling in Ecosystems"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0038858"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0038858", "name": "item", "description": "10.1371/journal.pone.0038858", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0038858"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-06-11T00:00:00Z"}}, {"id": "10.1371/journal.pone.0061188", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:31Z", "type": "Journal Article", "created": "2013-04-12", "title": "Interactive Effects Of Nitrogen And Phosphorus On Soil Microbial Communities In A Tropical Forest", "description": "Elevated nitrogen (N) deposition in humid tropical regions may exacerbate phosphorus (P) deficiency in forests on highly weathered soils. However, it is not clear how P availability affects soil microbes and soil carbon (C), or how P processes interact with N deposition in tropical forests. We examined the effects of N and P additions on soil microbes and soil C pools in a N-saturated old-growth tropical forest in southern China to test the hypotheses that (1) N and P addition will have opposing effects on soil microbial biomass and activity, (2) N and P addition will alter the composition of the microbial community, (3) the addition of N and P will have interactive effects on soil microbes and (4) addition-mediated changes in microbial communities would feed back on soil C pools. Phospholipid fatty acid (PLFA) analysis was used to quantify the soil microbial community following four treatments: Control, N addition (15 g N m(-2) yr(-1)), P addition (15 g P m(-2) yr(-1)), and N&P addition (15 g N m(-2) yr(-1) plus 15 g P m(-2) yr(-1)). These were applied from 2007 to 2011. Whereas additions of P increased soil microbial biomass, additions of N reduced soil microbial biomass. These effects, however, were transient, disappearing over longer periods. Moreover, N additions significantly increased relative abundance of fungal PLFAs and P additions significantly increased relative abundance of arbuscular mycorrhizal (AM) fungi PLFAs. Nitrogen addition had a negative effect on light fraction C, but no effect on heavy fraction C and total soil C. In contrast, P addition significantly decreased both light fraction C and total soil C. However, there were no interactions between N addition and P addition on soil microbes. Our results suggest that these nutrients are not co-limiting, and that P rather than N is limiting in this tropical forest.", "keywords": ["2. Zero hunger", "China", "Tropical Climate", "Bacteria", "Nitrogen", "Science", "Q", "Fatty Acids", "R", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "Trees", "Soil", "13. Climate action", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Fertilizers", "Phospholipids", "Soil Microbiology", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0061188"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0061188", "name": "item", "description": "10.1371/journal.pone.0061188", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0061188"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-04-12T00:00:00Z"}}, {"id": "10.1371/journal.pone.0096182", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:19:32Z", "type": "Journal Article", "created": "2014-05-09", "title": "Biotic And Abiotic Properties Mediating Plant Diversity Effects On Soil Microbial Communities In An Experimental Grassland", "description": "Plant diversity drives changes in the soil microbial community which may result in alterations in ecosystem functions. However, the governing factors between the composition of soil microbial communities and plant diversity are not well understood. We investigated the impact of plant diversity (plant species richness and functional group richness) and plant functional group identity on soil microbial biomass and soil microbial community structure in experimental grassland ecosystems. Total microbial biomass and community structure were determined by phospholipid fatty acid (PLFA) analysis. The diversity gradient covered 1, 2, 4, 8, 16 and 60 plant species and 1, 2, 3 and 4 plant functional groups (grasses, legumes, small herbs and tall herbs). In May 2007, soil samples were taken from experimental plots and from nearby fields and meadows. Beside soil texture, plant species richness was the main driver of soil microbial biomass. Structural equation modeling revealed that the positive plant diversity effect was mainly mediated by higher leaf area index resulting in higher soil moisture in the top soil layer. The fungal-to-bacterial biomass ratio was positively affected by plant functional group richness and negatively by the presence of legumes. Bacteria were more closely related to abiotic differences caused by plant diversity, while fungi were more affected by plant-derived organic matter inputs. We found diverse plant communities promoted faster transition of soil microbial communities typical for arable land towards grassland communities. Although some mechanisms underlying the plant diversity effect on soil microorganisms could be identified, future studies have to determine plant traits shaping soil microbial community structure. We suspect differences in root traits among different plant communities, such as root turnover rates and chemical composition of root exudates, to structure soil microbial communities.", "keywords": ["0301 basic medicine", "570", "Chromatography", " Gas", "Geography & travel", "Science", "910", "Plant Roots", "Gas Chromatography-Mass Spectrometry", "Soil", "03 medical and health sciences", "info:eu-repo/classification/ddc/910", "Biomass", "Phospholipids", "Soil Microbiology", "2. Zero hunger", "0303 health sciences", "Bacteria", "Q", "Fatty Acids", "R", "Fungi", "Water", "Biodiversity", "Models", " Theoretical", "Plants", "15. Life on land", "ddc:910", "Grassland", "13. Climate action", "Medicine", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0096182"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0096182", "name": "item", "description": "10.1371/journal.pone.0096182", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0096182"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-05-09T00:00:00Z"}}, {"id": "10.2134/jeq2004.0470", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:12Z", "type": "Journal Article", "created": "2005-09-09", "title": "Microbial Response To Heavy Metal-Polluted Soils: Community Analysis From Phospholipid-Linked Fatty Acids And Ester-Linked Fatty Acids Extracts", "description": "ABSTRACT

Heavy metal pollution of soil is of concern for human health and ecosystem function. The soil microbial community should be a sensitive indicator of metal contamination effects on bioavailability and biogeochemical processes. Simple methods are needed to determine the degree of in situ pollution and effectiveness of remediating metal\uffe2\uff80\uff90contaminated soils. Currently, phospholipid\uffe2\uff80\uff90linked fatty acids (PLFAs) are preferred for microbial profiling but this method is time consuming, whereas direct soil extraction and transesterification of total ester\uffe2\uff80\uff90linked fatty acids (ELFAs) is attractive because of its simplicity. The 1998 mining acid\uffe2\uff80\uff93metal spill of >4000 ha in the Guadiamar watershed (southwestern Spain) provided a unique opportunity to study these two microbial lipid profiling methods. Replicated treatments were set up as nonpolluted, heavy metal polluted and reclaimed, and polluted soils. Inferences from whole community\uffe2\uff80\uff93diversity analysis and correlations of individual fatty acids with metals suggested Cu, Cd, and Zn were the most important in affecting microbial community structure, along with pH. The microbial stress marker, monounsaturated fatty acids, was significantly lower for reclaimed and polluted soil over nonpolluted soils for both PLFA and ELFA extraction. Another stress marker, the monounsaturated to saturated fatty acids ratio, only showed this for the PLFA. The general fungal marker (18:2\uffcf\uff896c), the arbuscule mycorrhizae marker (16:1\uffcf\uff895c), and iso\uffe2\uff80\uff90 and anteiso\uffe2\uff80\uff90branched PLFAs (Gram positive bacteria) were suppressed with increasing pollution whereas 17:0cy (Gram negative bacteria) increased with metal pollution. For both extraction methods, richness and diversity were greater in nonpolluted soils and lowest in polluted soils. The ELFA method was sensitive for reflecting metal pollution on microbial communities and could be suitable for routine use in ecological monitoring and risk assessment programs because of its simplicity and reproducibility.

", "keywords": ["2. Zero hunger", "Bacteria", "Fatty Acids", "Fungi", "Esters", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water", "Soil", "Biodegradation", " Environmental", "Spain", "13. Climate action", "Metals", " Heavy", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Phospholipids", "Soil Microbiology", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10.2134/jeq2004.0470"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2004.0470", "name": "item", "description": "10.2134/jeq2004.0470", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2004.0470"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-09-01T00:00:00Z"}}, {"id": "10.3168/jds.2009-2379", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:43Z", "type": "Journal Article", "created": "2010-03-19", "title": "Effect Of Saccharomyces Cerevisiae Fermentation Product On Ruminal Fermentation And Nutrient Utilization In Dairy Cows", "description": "The goal of this experiment was to investigate the effect of yeast culture (Saccharomyces cerevisiae) on rumen fermentation, nutrient utilization, and ammonia and methane emission from manure in dairy cows. Eight ruminally cannulated Holstein cows were allocated to 2 dietary treatments in a crossover design. Treatments were control (no yeast culture) and XP (yeast culture, fed at 56 g/head per day; XP, Diamond V Mills Inc., Cedar Rapids, IA). Dry matter intake, milk yield, milk composition, and body weight were similar between treatments. Milk urea nitrogen concentration was also not affected by treatment. Rumen pH was similar between the control and XP treatments, but rumen ammonia concentration tended to be lower with XP than with the control. Treatment had no effect on concentrations of total or individual volatile fatty acids, protozoal counts, polysaccharide-degrading activities (except amylase activity that tended to be increased by XP), or methane production in the rumen. Urinary N losses did not differ significantly between treatments, but allantoin and total purine derivative excretions and the estimated microbial N outflow from the rumen tended to be increased by XP compared with the control treatment. Total-tract apparent digestibility of dietary nutrients was not affected by XP. Milk fatty acid composition was also not altered by XP supplementation. Cumulative (253 h) ammonia and methane emissions from manure, measured in a steady-state gas emission system, were slightly decreased by XP. Overall, the yeast culture tested had little effect on ruminal fermentation, digestibility, or N losses, but tended to reduce rumen ammonia concentration and increase microbial protein synthesis in the rumen, and decreased ammonia and methane emissions from manure.", "keywords": ["2. Zero hunger", "Cross-Over Studies", "Rumen", "Nitrogen", "Fatty Acids", "0402 animal and dairy science", "Saccharomyces cerevisiae", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "Diet", "0403 veterinary science", "Dairying", "Milk", "Ammonia", "Purines", "13. Climate action", "Fermentation", "Animals", "Animal Nutritional Physiological Phenomena", "Cattle", "Female", "Allantoin"]}, "links": [{"href": "https://doi.org/10.3168/jds.2009-2379"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Dairy%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3168/jds.2009-2379", "name": "item", "description": "10.3168/jds.2009-2379", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3168/jds.2009-2379"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-02-01T00:00:00Z"}}, {"id": "10.3168/jds.2010-3281", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:43Z", "type": "Journal Article", "created": "2010-11-21", "title": "Nitrate And Sulfate: Effective Alternative Hydrogen Sinks For Mitigation Of Ruminal Methane Production In Sheep", "description": "Twenty male crossbred Texel lambs were used in a 2 \u00d7 2 factorial design experiment to assess the effect of dietary addition of nitrate (2.6% of dry matter) and sulfate (2.6% of dry matter) on enteric methane emissions, rumen volatile fatty acid concentrations, rumen microbial composition, and the occurrence of methemoglobinemia. Lambs were gradually introduced to nitrate and sulfate in a corn silage-based diet over a period of 4 wk, and methane production was subsequently determined in respiration chambers. Diets were given at 95% of the lowest ad libitum intake observed within one block in the week before methane yield was measured to ensure equal feed intake of animals between treatments. All diets were formulated to be isonitrogenous. Methane production decreased with both supplements (nitrate: -32%, sulfate: -16%, and nitrate+sulfate: -47% relative to control). The decrease in methane production due to nitrate feeding was most pronounced in the period immediately after feeding, whereas the decrease in methane yield due to sulfate feeding was observed during the entire day. Methane-suppressing effects of nitrate and sulfate were independent and additive. The highest methemoglobin value observed in the blood of the nitrate-fed animals was 7% of hemoglobin. When nitrate was fed in combination with sulfate, methemoglobin remained below the detection limit of 2% of hemoglobin. Dietary nitrate decreased heat production (-7%), whereas supplementation with sulfate increased heat production (+3%). Feeding nitrate or sulfate had no effects on volatile fatty acid concentrations in rumen fluid samples taken 24h after feeding, except for the molar proportion of branched-chain volatile fatty acids, which was higher when sulfate was fed and lower when nitrate was fed, but not different when both products were included in the diet. The total number of rumen bacteria increased as a result of sulfate inclusion in the diet. The number of methanogens was reduced when nitrate was fed. Enhanced levels of sulfate in the diet increased the number of sulfate-reducing bacteria. The number of protozoa was not affected by nitrate or sulfate addition. Supplementation of a diet with nitrate and sulfate is an effective means for mitigating enteric methane emissions from sheep.", "keywords": ["Male", "Rumen", "reduction", "in-vitro", "nitrogen", "Random Allocation", "fumaric-acid", "Animals", "Intestinal Mucosa", "real-time pcr", "2. Zero hunger", "rumen", "Nitrates", "Sheep", "Sulfates", "emissions", "0402 animal and dairy science", "methanogenesis", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "Fatty Acids", " Volatile", "Diet", "Agricultural Land Management", "cattle", "Dietary Supplements", "Fermentation", "Animal Nutritional Physiological Phenomena", "feed-intake", "Methemoglobinemia", "Methane"]}, "links": [{"href": "https://doi.org/10.3168/jds.2010-3281"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Dairy%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3168/jds.2010-3281", "name": "item", "description": "10.3168/jds.2010-3281", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3168/jds.2010-3281"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-12-01T00:00:00Z"}}, {"id": "10.2139/ssrn.4106102", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:29Z", "type": "Journal Article", "created": "2022-05-28", "title": "Evaluation of Biostimulation, Bioaugmentation, and Organic Amendments Application on the Bioremediation of Recalcitrant Hydrocarbons of Soil", "description": "In the present work, the operational conditions for improving the degradation rates of Total Petroleum Hydrocarbons (TPHs) in contaminated soil from a machinery park were optimized at a microcosms scale along a 90- days incubation period. In this study, bioremediation strategies and an organic amendment have been tested to verify the remediation of soil contaminated with different hydrocarbons, mineral oils, and heavy metals. Specifically, designed biostimulation and bioaugmentation strategies were compared with and without adding vermicompost. The polluted soil harboring multiple contaminants, partially attenuated for years, was used. The initial profile showed enrichment in heavy linear alkanes, suggesting a previous moderate weathering. The application of vermicompost increased five and two times the amounts of available phosphorus (P) and exchangeable potassium (K), respectively, as a direct consequence of the organic amendment addition. The microbial activity increased due to soil acidification, which influenced the solubility of P and other micronutrients. It also impacted the predominance and variability of the different microbial groups and the incubation, as reflected by phospholipid fatty acid (PLFA) results. An increase in the alkaline phosphatases and proteases linked to bacterial growth was displayed. This stimulation of microbial metabolism correlated with the degradation rates since TPHs degradation\u2019 efficiency after vermicompost addition reached 32.5% and 34.4% of the initial hydrocarbon levels for biostimulation and bioaugmentation, respectively. Although Polycyclic Aromatic", "keywords": ["Soil enzymes", "01 natural sciences", "Total petroleum hydrocarbons polluted soils", "Soil", "Bioaugmentation", "Alkanes", "Soil Pollutants", "Micronutrients", "Polycyclic Aromatic Hydrocarbons", "Materials", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences", "2. Zero hunger", "Minerals", "Materiales", "Biostimulation", " Bioaugmentation", " Phospholipid fatty acids", " Total petroleum hydrocarbons polluted soils", " Soil enzymes", "Fatty Acids", "Phosphorus", "Phenanthrenes", "Hydrocarbons", "Phosphoric Monoester Hydrolases", "6. Clean water", "Biostimulation", "Biodegradation", " Environmental", "Petroleum", "13. Climate action", "Phospholipid fatty acids", "Potassium", "Oils", "Biostimulation Bioaugmentation Phospholipid fatty acids Total petroleum hydrocarbons polluted soils Soil enzymes", "Peptide Hydrolases"]}, "links": [{"href": "https://doi.org/10.2139/ssrn.4106102"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/SSRN%20Electronic%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2139/ssrn.4106102", "name": "item", "description": "10.2139/ssrn.4106102", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2139/ssrn.4106102"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}, {"id": "10.2307/2640985", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:34Z", "type": "Journal Article", "created": "2006-04-17", "description": "Elevated atmospheric CO2 has the potential to increase the production and alter the chemistry of organic substrates entering soil from plant production, the magnitude of which is constrained by soil-N availability. Because microbial growth in soil is limited by substrate inputs from plant production, we reasoned that changes in the amount and chemistry of these organic substrates could affect the composition of soil microbial com- munities and the cycling of N in soil. We studied microbial community composition and soil-N transformations beneath Populus tremuloides Michx. growing under experimental atmospheric CO2 (35.7 and 70.7 Pa) and soil-N-availability (low N 5 61 ng N\u00b7g 21 \u00b7d 21 and high N 5 319 ng N\u00b7g 21 \u00b7d 21 ) treatments. Atmospheric CO2 concentration was modified in large, open-top chambers, and we altered soil-N availability in open-bottom root boxes by mixing different proportions of A and C horizon material. We used phospholipid fatty-acid analysis to gain insight into microbial community composition and coupled this analysis to measurements of soil-N transformations using 15 N-pool dilution techniques. The infor- mation presented here is part of an integrated experiment designed to elucidate the phys- iological mechanisms controlling the flow of C and N in the plant-soil system. Our ob- jectives were (1) to determine whether changes in plant growth and tissue chemistry alter microbial community composition and soil-N cycling in response to increasing atmospheric CO2 and soil-N availability and (2) to integrate the results of our experiment into a synthesis of elevated atmospheric CO2 and the cycling of C and N in terrestrial ecosystems. After 2.5 growing seasons, microbial biomass, gross N mineralization, microbial im- mobilization, and nitrification (gross and net) were equivalent at ambient and elevated CO2, suggesting that increases in fine-root production and declines in fine-root N concentration were insufficient to alter the influence of native soil organic matter on microbial physiology; this was the case in both low- and high-N soil. Similarly, elevated CO2 did not alter the proportion of bacterial, actinomycetal, or fungal phospholipid fatty acids in low-N or high-N soil, indicating that changes in substrate input from greater plant growth under elevated CO2 did not alter microbial community composition. Our results differ from a substantial number of studies reporting increases and decreases in soil-N cycling under elevated CO 2. From our analysis, it appears that soil-N cycling responds to elevated atmospheric CO 2 in experimental situations where plant roots have fully colonized the soil and root-associated C inputs are sufficient to modify the influence of native soil organic matter on microbial physiology. In young developing ecosystems where plant roots have not fully exploited the soil, microbial metabolism appears to be regulated by relatively large pools of soil organic matter, rather than by the additional input of organic substrates under elevated CO 2.", "keywords": ["measurement-", "soil microorganisms", "Ecology and Evolutionary Biology", "nitrogen-: cycling-", "feedback", "microbial community composition", "techniques-", "Environmental-Sciences)", "01 natural sciences", "litter-plant", "biomass-", "gross and net", "124-38-9: CARBON DIOXIDE", "Spermatophytes-", "cycling-", "soil-organic-matter", "mineralization", "Spermatophyta-", "responses-", "phospholipid-fatty-acids", "2. Zero hunger", "Climatology- (Environmental-Sciences)", "Angiosperms-", "Angiospermae-", "Plants-", "global climate change", "microbial immobilization", "nutrient-", "Soil-Science", "6. Clean water", "metabolism-", "soil-N transformations", "transformation-", "substrates-", "7727-37-9: NITROGEN", "atmosphere-", "elevated atmospheric", "570", "nitrification-", "nitrogen immobilization", "Science", "Vascular-Plants", "poplars-", "phospholipid fatty acids (PFLAs)", "carbon-dioxide", "growth-", "soil-microbial-community-composition", "Salicaceae-: Dicotyledones-", "microbial-flora", "Populus tremuloides", "Plantae-", "organic-matter", "consortia-", "0105 earth and related environmental sciences", "communities-", "ecosystem", "analysis-", "atmospheric CO2 and soil-N availability", "soil-availability", "mineralization-", "carbon dioxide", "fatty-acids", "15. Life on land", "substrate-input", "Populus-tremuloides (Salicaceae-)", "13. Climate action", "roots-", "Terrestrial-Ecology (Ecology-", "composition-", "Dicots-", "immobilization-", "seasons-", "ecosystems-"], "contacts": [{"organization": "Zak, Donald R., Pregitzer, Kurt S., Curtis, Peter S., Holmes, William E.,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.2307/2640985"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecological%20Applications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2307/2640985", "name": "item", "description": "10.2307/2640985", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2307/2640985"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2000-02-01T00:00:00Z"}}, {"id": "10.2527/1996.7451144x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:38Z", "type": "Journal Article", "created": "2016-08-09", "description": "The effects of alfalfa saponins on ruminal pH and VFA concentrations, protozoal numbers, forestomach motility, and digesta flow from the rumen were examined in this experiment. In a preliminary study, either 800 or 1600 mg/kg of saponins per kg BW were administered intraruminally in a single dose to one of two 60-kg wethers. Ruminal contractions were suppressed within 15 min. The study was terminated when one wether didn't recover. Saponins were then administered intraruminally in two equal doses daily into four ruminally and duodenally cannulated wethers (60 +/- 1 kg) in amounts equivalent to 0, 200, 400, and 800 mg/kg BW daily in a 4 x 4 Latin Square arrangement. Saponins increased VFA concentrations (P = .001) and lowered ruminal pH (P = .001) after 2 d of administration. On d 2 and d 14, saponins decreased (P < .01) protozoal populations. On d 11, pressure changes during ruminal contractions increased by 79% during resting (P = .06), 202% during feeding (P = .05), and 53% during an 8-h period (P = .08) as the level of saponins increased from 0 to 800 mg/kg BW. Administered saponins increased duodenal flows of OM and total N by 19 and 26%, respectively (P = .04 and .01). Apparent total tract N digestibility was reduced (P = .02) by 5% at the highest saponin dosage. We conclude that alfalfa may contain enough saponins to adversely impact ruminoreticular motility. Saponins also caused decreased ruminal protozoal populations, increased flow of total duodenal N, and reduced ruminal and total tract apparent digestibilities.", "keywords": ["Male", "2. Zero hunger", "Rumen", "Sheep", "Dose-Response Relationship", " Drug", "0402 animal and dairy science", "04 agricultural and veterinary sciences", "Carbon Dioxide", "Saponins", "Fatty Acids", " Volatile", "Oxygen Consumption", "Intestine", " Small", "Animals", "Digestion", "Gastrointestinal Motility", "Methane", "Medicago sativa"], "contacts": [{"organization": "P T Klita, T W Fenton, G. W. Mathison, R. T. Hardin,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.2527/1996.7451144x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Animal%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2527/1996.7451144x", "name": "item", "description": "10.2527/1996.7451144x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2527/1996.7451144x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1996-01-01T00:00:00Z"}}, {"id": "10.2527/1998.763906x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:38Z", "type": "Journal Article", "created": "2016-08-09", "description": "We measured effects of continuous vs twice-daily feeding, the addition of unsaturated fat to the diet, and monensin on milk production, milk composition, feed intake, and CO2-methane production in four experiments in a herd of 88 to 109 milking Holsteins. Methane and CO2 production increased with twice-daily feeding, but the CO2:CH4 ratio remained unchanged. Soybean oil did not affect the milkfat percentages, but fatty acid composition was changed. All saturated fatty acids up to and including 16:0 decreased (P < .01), whereas 18:0 and trans 18:1 increased (P < .001). The 18:2 conjugated dienes also increased (P < .01) when the cows were fed soybean oil. Monensin addition to the diet at 24 ppm decreased methane production (P < .01); the CO2:CH4 ratios reached 15, milk production increased (P < .01), and milkfat percentage and total milkfat output decreased (P < .01), as did feed consumption, compared with cows fed diets without monensin (P < .05). Milk fatty acid composition showed evidence of depressed ruminal biohydrogenation: saturated fatty acids (P < .05) decreased and 18:1 increased (P < .001); most of the increase was seen in the trans 18:1 isomer. As with soybean oil feeding, addition of monensin also increased (P < .05) the concentration of conjugated dienes. The monensin feeding trial was repeated 161 d later with 88 cows, of which 67 received monensin in the diet in the first trial and 21 cows were newly freshened and had never received monensin. Methane production again decreased (P < .05), but this time the CO2:CH4 ratio did not change and all other monensin-related effects were absent. The ruminal microflora in the cows that had previously received monensin seemed to have undergone some adaptive changes and no longer responded as before.", "keywords": ["2. Zero hunger", "Rumen", "Time Factors", "0402 animal and dairy science", "04 agricultural and veterinary sciences", "Fatty Acids", " Volatile", "Animal Feed", "Gastrointestinal Contents", "0403 veterinary science", "Milk", "Dietary Fats", " Unsaturated", "Food", " Fortified", "Animals", "Lactation", "Cattle", "Female", "Monensin", "Methane"]}, "links": [{"href": "https://doi.org/10.2527/1998.763906x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Animal%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2527/1998.763906x", "name": "item", "description": "10.2527/1998.763906x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2527/1998.763906x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1998-01-01T00:00:00Z"}}, {"id": "10.2527/2004.8261839x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:39Z", "type": "Journal Article", "created": "2016-08-09", "description": "The effects of alpha-cyclodextrin-horseradish oil complex (CD-HR) on methane production and ruminal fermentation were studied in vitro and in steers. In the in vitro study, diluted ruminal fluid (30 mL) was incubated anaerobically at 38 degrees C for 6 h with or without CD-HR, using cornstarch as substrate. The CD-HR was added at various concentrations (0, 0.17, 0.85 and 1.7 g/L). Treatment affected neither the pH of the medium nor the number of protozoa. Total VFA increased in a linear manner (P = 0.02), and NH3-N decreased quadratically (P = 0.04) as the concentration of CD-HR increased from 0.17 g/L to 1.7 g/L. Molar proportions of acetate decreased in a linear manner (P = 0.03), and propionate increased linearly (P = 0.008) with increasing concentrations of CD-HR. Production of methane was inhibited up to 90%, whereas accumulation of dihydrogen was increased 36-fold by 1.7 g/L of CD-HR supplementation relative to controls. The effect of CD-HR on methane production, ruminal fermentation and microbes, and digestibility was further investigated in vivo using four Holstein steers in a crossover design. The CD-HR supplement was mixed into the concentrate portion of a (1.5:1) Sudangrass hay plus concentrate mixture that was fed twice daily to the steers. Ruminal samples were collected 0, 2, and 5 h after the morning feeding. No effects of CD-HR supplementation on ruminal pH (P = 0.63) or protozoal numbers (P = 0.44) were observed. Molar proportion of acetate was decreased (P = 0.04) and propionate was increased (P = 0.005) by CD-HR treatment. Molar proportion of butyrate was increased (P = 0.05) in CD-HR-supplemented steers. Ruminal NH3-N was decreased (P = 0.05) by treatment. Blood plasma glucose concentration was increased (P = 0.02) and urea-N was decreased (P = 0.04) with CD-HR supplementation. Daily DMI was decreased (P = 0.04), and apparent digestibility of DM (P = 0.13), NDF (P = 0.14), and CP tended (P = 0.14) to be increased by treatment. Methane production was decreased (P = 0.03) by 19%, and the number of methanogens was also decreased (P = 0.03). Although N retention (P = 0.11), total viable bacteria (P = 0.15), and sulfate-reducing bacteria (P = 0.17) were not significantly altered by treatment, tendencies for increases were noted with CD-HR supplementation. The number of cellulolytic (P = 0.38) and acetogenic bacteria (P = 0.32) remained unchanged by treatment. These results indicate that CD-HR supplementation can be used to decrease methane production in steers.", "keywords": ["Male", "Cyclodextrins", "Wasabia", "Cross-Over Studies", "Rumen", "Dose-Response Relationship", " Drug", "0402 animal and dairy science", "Eukaryota", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "In Vitro Techniques", "Fatty Acids", " Volatile", "6. Clean water", "Bacteria", " Anaerobic", "Random Allocation", "Fermentation", "Animals", "Plant Oils", "Cattle", "Digestion", "Energy Intake", "Methane"], "contacts": [{"organization": "Z. A. Lila, N. Ajisaka, K. Hara, Koji Hara, Nazimuddin Mohammed, S. Kanda, K. Mikuni, H. Itabashi,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.2527/2004.8261839x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Animal%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2527/2004.8261839x", "name": "item", "description": "10.2527/2004.8261839x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2527/2004.8261839x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2004-06-01T00:00:00Z"}}, {"id": "10.2527/2005.831182x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:39Z", "type": "Journal Article", "created": "2016-08-09", "description": "The objective of this study was to compare methane emission by goats consuming the condensed tannin-containing forage sericea lespedeza (Les-pedeza cuneata) or a mixture of crabgrass (Digitaria ischaemum) and Kentucky 31 tall fescue (Festuca arundinacea). Two groups of 12 Angora does (initial average BW = 41.5 +/- 2.7 kg) that previously grazed a pasture of sericea lespedeza or crabgrass/tall fescue for approximately 4 mo were used. After 1 wk of adaptation to metabolism cages, gas exchange was measured for 24 h in an open-circuit respiration calorimetry system with four head boxes. Forage harvested daily from the previously grazed pastures was consumed ad libitum. Crude protein concentration was 10.3 and 13.0%, IVDMD was 64.5 and 75.3%, and the level of condensed tannins was 17.7 and 0.5% for sericea lespedeza and crabgrass/tall fescue, respectively. Dry matter intake (1.11 vs. 0.67 kg/d) and digestible DMI (estimated from IVDMD; 0.71 vs. 0.51 kg/d) were greater (P < 0.01) for sericea lespedeza than for crabgrass/tall fescue. Ruminal ammonia N (3.7 and 9.9 mg/dL; P < 0.001) and plasma urea-N concentrations (16.7 and 20.9 mg/dL; P = 0.07) were lower for sericea lespedeza than for crabgrass/tall fescue. Concentrations of individual and total VFA and the acetate-to-propionate ratio in ruminal fluid did not differ between treatments (P > 0.19). Despite higher DMI by goats fed sericea lespedeza, daily energy expenditure (409 vs. 431 kJ/kg BW(0.75)), heart rate (70 vs. 73 beats/min), and the ratio of energy expenditure to heart rate (5.82 vs. 5.94) did not differ between sericea lespedeza and crabgrass/tall fescue, respectively (P > 0.13). Methane emission expressed as both quantity per day or relative to DMI was lower (P <0.001) for sericea lespedeza than for crabgrass/tall fescue (7.4 vs. 10.6 g/d and 6.9 vs. 16.2 g/kg DMI). Substantial differences between the forages in condensed tannins concentration and methane emission by Angora goats suggest that condensed tannins decreased methane emission.", "keywords": ["Festuca", "2. Zero hunger", "Rumen", "Pulmonary Gas Exchange", "Goats", "Body Weight", "0402 animal and dairy science", "Lespedeza", "04 agricultural and veterinary sciences", "Fatty Acids", " Volatile", "Animal Feed", "Blood Urea Nitrogen", "Eating", "Ammonia", "13. Climate action", "Digitaria", "Animals", "Animal Nutritional Physiological Phenomena", "Female", "Proanthocyanidins", "Methane"]}, "links": [{"href": "https://doi.org/10.2527/2005.831182x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Animal%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2527/2005.831182x", "name": "item", "description": "10.2527/2005.831182x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2527/2005.831182x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-01-01T00:00:00Z"}}, {"id": "10.2527/jas.2009-1786", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:39Z", "type": "Journal Article", "created": "2009-06-20", "title": "Effect Of Sward Dry Matter Digestibility On Methane Production, Ruminal Fermentation, And Microbial Populations Of Zero-Grazed Beef Cattle", "description": "Increasing the digestibility of pasture for grazing ruminants has been proposed as a low-cost practical means of reducing ruminant CH(4) emissions. At high feed intake levels, the proportion of energy lost as CH(4) decreases as the digestibility of the diet increases. Therefore, improving forage digestibility may improve productivity as DM and energy intake are increased. A zero-grazing experiment was conducted to determine the effect of sward DM digestibility (DMD) on DMI, CH(4) emissions, and indices of rumen fermentation of beef animals. Twelve Charolais-cross heifers were assigned to 1 of 2 treatments, with 6 heifers per dietary treatment. Additionally, 4 cannulated Aberdeen Angus-cross steers were randomly allocated to each of these 2 treatments in a crossover design. Dietary treatments consisted of swards managed to produce (i) high digestibility pasture (high DMD) or (ii) pasture with less digestibility (low DMD), both offered for ad libitum intake. All animals were zero-grazed and offered freshly cut herbage twice daily. In vitro DMD values for the high and low DMD swards were 816 and 706 g/kg of DM. Heifers offered the high DMD grass had greater (P < 0.001) daily DMI of 7.66 kg compared with 5.38 kg for those offered the low DMD grass. Heifers offered the high DMD grass had greater (P = 0.003) daily CH(4) production (193 g of CH(4)/d) than those offered the low DMD grass (138 g of CH(4)/d). However, when corrected for DMI, digestible DMI, or ingested gross energy, there was no difference (P > 0.05) in CH(4) production between dietary treatments. For cannulated steers, intake tended (P = 0.06) to be greater for the high DMD grass (5.56 vs. 4.27 kg of DM/d), but rumen protozoa (4.95 x 10(4)/mL; P = 0.62); rumen ammonia (34 mg of N/L; P = 0.24); rumen total VFA (103 mM; P = 0.58), and rumen pH (6.8; P = 0.43) did not differ between treatments. There was no difference in total bacteria numbers, relative expression of the mcrA gene, and numbers of cycles to threshold for fungi when determined using quantitative PCR between dietary treatments with mean values of 73.0 ng/microL, 0.958, and 21.75 C(T), respectively. Results of this study demonstrate that there was no difference in CH(4) production when corrected for intake or rumen fermentation variables of beef cattle offered a high or low digestibility sward.", "keywords": ["DNA", " Bacterial", "Male", "2. Zero hunger", "Rumen", "0402 animal and dairy science", "04 agricultural and veterinary sciences", "Fatty Acids", " Volatile", "Polymerase Chain Reaction", "Random Allocation", "Ammonia", "RNA", " Ribosomal", " 16S", "Lolium", "Animals", "Cattle", "Digestion", "Female", "Least-Squares Analysis", "Methane"]}, "links": [{"href": "https://doi.org/10.2527/jas.2009-1786"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Animal%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2527/jas.2009-1786", "name": "item", "description": "10.2527/jas.2009-1786", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2527/jas.2009-1786"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2009-06-19T00:00:00Z"}}, {"id": "10.3390/f7020045", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:20:58Z", "type": "Journal Article", "created": "2016-02-19", "description": "

Microbial-mediated decomposition and nutrient mineralization are major drivers of forest productivity. As landscape-scale fuel reduction treatments are being implemented throughout the fire-prone western United States of America, it is important to evaluate operationally how these wildfire mitigation treatments alter belowground processes. We quantified these important belowground components before and after management-applied fuel treatments of thinning alone, thinning combined with prescribed fire, and prescribed fire in ponderosa pine (Pinus ponderosa) stands at the Southwest Plateau, Fire and Fire Surrogate site, Arizona. Fuel treatments did not alter pH, total carbon and nitrogen (N) concentrations, or base cations of the forest floor (O horizon) or mineral soil (0\uffe2\uff80\uff935 cm) during this 2-year study. In situ rates of net N mineralization and nitrification in the surface mineral soil (0\uffe2\uff80\uff9315 cm) increased 6 months after thinning with prescribed fire treatments; thinning only resulted in net N immobilization. The rates returned to pre-treatment levels after one year. Based on phospholipid fatty acid composition, microbial communities in treated areas were similar to untreated areas (control) in the surface organic horizon and mineral soil (0\uffe2\uff80\uff935 cm) after treatments. Soil potential enzyme activities were not significantly altered by any of the three fuel treatments. Our results suggest that a variety of one-time alternative fuel treatments can reduce fire hazard without degrading soil fertility.

", "keywords": ["2. Zero hunger", "Ecology", "Life on Land", "fuel treatments", "13. Climate action", "Forestry Sciences", "fuel treatments; nitrification; nitrogen mineralization; phospholipid fatty acids; soil enzymes", "Plant Biology", "phospholipid fatty acids", "15. Life on land", "soil enzymes", "nitrification", "nitrogen mineralization"], "contacts": [{"organization": "Overby, Steven T, Hart, Stephen C,", "roles": ["creator"]}]}, "links": [{"href": "http://www.mdpi.com/1999-4907/7/2/45/pdf"}, {"href": "https://escholarship.org/content/qt54s4m5pt/qt54s4m5pt.pdf"}, {"href": "https://doi.org/10.3390/f7020045"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Forests", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/f7020045", "name": "item", "description": "10.3390/f7020045", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/f7020045"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-02-18T00:00:00Z"}}, {"id": "10.5061/dryad.rbnzs7hf0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:21:37Z", "type": "Dataset", "title": "Biochar and nitrogen fertilizer promote rice yield by altering soil enzyme activity and microbial community structure", "description": "unspecifiedBiochar can significantly change soil properties and improve soil quality. However, the effects of long-term combined application of biochar (B) and nitrogen (N) fertilizer on relationships between soil enzyme activity, microbial community structure and crop yield are still obscure. We characterized these relationships in a long-term (8 years) field experiment with rice, two biochar rates of 0 and 13.5 t ha-1 year-1 (B0 and B) and two N fertilizer rates of 0 and 300 kg N ha-1 year-1 (N0 and N). The repeated, long-term combined applications of biochar and N fertilizer significantly increased microbial biomass carbon and nitrogen (MBC and MBN), but biochar decreased the abundance of total bacteria, fungi, actinomycetes, Gram-positive and Gram-negative bacteria as well as the amount of total phospholipid fatty acids. The activity of leucine aminopeptidase (LAP) decreased significantly in the biochar-amended and N fertilized treatment, but the LAP activity either remained unchanged or increased with biochar amendment at N0. The relative abundance of bacterial phylum Chloroflexi was increased in the combined biochar and N fertilizer treatment. The changes in soil organic matter and the activity of \u03b1-1,4-xylosidase were the major properties influencing soil bacterial community composition, whereas the structure of fungal community was governed by MBC, MBN and LAP activity. In addition, long-term biochar and N fertilizer applied together significantly increased rice yield (more than biochar and nitrogen fertilizer applied alone). Yield was significantly positively correlated with LAP activity, but significantly negatively correlated with the relative abundance of Chloroflexi. In conclusion, long-term biochar and nitrogen fertilizer applications increased rice yield, which was associated with altered soil microbial community and enhanced activity of some enzymes.", "keywords": ["2. Zero hunger", "Microbial community", "FOS: Agricultural sciences", "biochar", "phospholipid fatty acids", "15. Life on land", "6. Clean water", "long-term experiment", "nitrogen fertilizer", "enzyme activity", "rice yield"], "contacts": [{"organization": "Zhang, Aiping", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.rbnzs7hf0"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.rbnzs7hf0", "name": "item", "description": "10.5061/dryad.rbnzs7hf0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.rbnzs7hf0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-09-01T00:00:00Z"}}, {"id": "10072/428410", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:24:36Z", "type": "Journal Article", "created": "2023-10-18", "title": "Community composition and physiological plasticity control microbial carbon storage across natural and experimental soil fertility gradients", "description": "Abstract

Many microorganisms synthesise carbon (C)-rich compounds under resource deprivation. Such compounds likely serve as intracellular C-storage pools that sustain the activities of microorganisms growing on stoichiometrically imbalanced substrates, making them potentially vital to the function of ecosystems on infertile soils. We examined the dynamics and drivers of three putative C-storage compounds (neutral lipid fatty acids [NLFAs], polyhydroxybutyrate [PHB], and trehalose) across a natural gradient of soil fertility in eastern Australia. Together, NLFAs, PHB, and trehalose corresponded to 8.5\uffe2\uff80\uff9340% of microbial C and 0.06\uffe2\uff80\uff930.6% of soil organic C. When scaled to \uffe2\uff80\uff9cstructural\uffe2\uff80\uff9d microbial biomass (indexed by polar lipid fatty acids; PLFAs), NLFA and PHB allocation was 2\uffe2\uff80\uff933-times greater in infertile soils derived from ironstone and sandstone than in comparatively fertile basalt- and shale-derived soils. PHB allocation was positively correlated with belowground biological phosphorus (P)-demand, while NLFA allocation was positively correlated with fungal PLFA : bacterial PLFA ratios. A complementary incubation revealed positive responses of respiration, storage, and fungal PLFAs to glucose, while bacterial PLFAs responded positively to PO43-. By comparing these results to a model of microbial C-allocation, we reason that NLFA primarily served the \uffe2\uff80\uff9creserve\uffe2\uff80\uff9d storage mode for C-limited taxa (i.e., fungi), while the variable portion of PHB likely served as \uffe2\uff80\uff9csurplus\uffe2\uff80\uff9d C-storage for P-limited bacteria. Thus, our findings reveal a convergence of community-level processes (i.e., changes in taxonomic composition that underpin reserve-mode storage dynamics) and intracellular mechanisms (e.g., physiological plasticity of surplus-mode storage) that drives strong, predictable community-level microbial C-storage dynamics across gradients of soil fertility and substrate stoichiometry.area.csv) Assignment of lipids that the measured fatty acids originate from (LipidClass.csv) An R script reproducing the calculations and plotting for Fig. 2 and Fig. S1 (pure_lipids.R) 2. Microbial pure culture fatty acid data data TIC area data of the PLFA, NLFA, and GLFA data from pure culture extracts (area.csv) Files needed for calculating the data and assigning taxonomic groups in the R code (weights.csv, C_atoms.csv, species_list.csv) An R script reproducing the calculations and plotting for Fig. 3, Fig. 4, Fig. S2, and Fig. S3 (pure_cultures.R) 3. Soil fatty acid data Absolute abundance data in nmol C g-1 dry weight of the PLFA, NLFA, and GLFA data from soil extracts (nmolC.csv) Taxonomic group assignments of fatty acids needed to run the R code (phylum.csv) An R script reproducing the calculations and plotting for Fig. 5, and Fig. S4 (soil.R)", "keywords": ["NLFA", "soil lipids", "soil fatty acid profiles", "phospholipid fatty acids", "GLFA"], "contacts": [{"organization": "Stefan, Gorka, Darcy, Sean, Horak, Julia, Imai, Bruna, Mohrlok, Moritz, Salas, Erika, Richter, Andreas, Schmidt, Hannes, Wanek, Wolfgang, Kaiser, Christina, Canarini, Alberto,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.8410341"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.8410341", "name": "item", "description": "10.5281/zenodo.8410341", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.8410341"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-05T00:00:00Z"}}, {"id": "10.5281/zenodo.8410340", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-25T16:23:51Z", "type": "Dataset", "title": "Dataset for the article \"Beyond PLFA: Concurrent extraction of neutral and glycolipid fatty acids provides new insights into soil microbial communities\"", "description": "The following are data and code used for statistical analysis and figure plotting in the manuscript Gorka et al. (2023) 'Beyond PLFA: Concurrent extraction of neutral and glycolipid fatty acids provides new insights into soil microbial communities', Soil Biology and Biochemistry It contains the following files: 1. Pure lipid standard data Total ion chromatogram (TIC) area data (area.csv) Assignment of lipids that the measured fatty acids originate from (LipidClass.csv) An R script reproducing the calculations and plotting for Fig. 2 and Fig. S1 (pure_lipids.R) 2. Microbial pure culture fatty acid data data TIC area data of the PLFA, NLFA, and GLFA data from pure culture extracts (area.csv) Files needed for calculating the data and assigning taxonomic groups in the R code (weights.csv, C_atoms.csv, species_list.csv) An R script reproducing the calculations and plotting for Fig. 3, Fig. 4, Fig. S2, and Fig. S3 (pure_cultures.R) 3. Soil fatty acid data Absolute abundance data in nmol C g-1 dry weight of the PLFA, NLFA, and GLFA data from soil extracts (nmolC.csv) Taxonomic group assignments of fatty acids needed to run the R code (phylum.csv) An R script reproducing the calculations and plotting for Fig. 5, and Fig. S4 (soil.R)", "keywords": ["NLFA", "soil lipids", "soil fatty acid profiles", "phospholipid fatty acids", "GLFA"], "contacts": [{"organization": "Gorka Stefan, Sean Darcy, Julia Horak, Bruna Imai, Moritz Mohrlok, Erika Salas, Andreas Richter, Hannes Schmidt, Wolfgang Wanek, Christina Kaiser, Alberto Canarini,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5281/zenodo.8410340"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.8410340", "name": "item", "description": "10.5281/zenodo.8410340", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.8410340"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-05T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Fatty+acids&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Fatty+acids&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Fatty+acids&", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Fatty+acids&offset=50", "hreflang": "en-US"}], "numberMatched": 55, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-07-26T10:09:20.848004Z"}