\uffe2\uff80\uff82To evaluate whether functional groups have a similar response to global change, the responses to CO2 concentration and N availability of grassland species from several functional groups are reported here.
\uffe2\uff80\uff82Sixteen perennial grassland species from four trait\uffe2\uff80\uff90based functional groups (C3 grasses, C4 grasses, non\uffe2\uff80\uff90leguminous forbs, legumes) were grown in field monocultures under ambient or elevated (560\uffc2\uffa0\uffc2\uffb5mol mol\uffe2\uff88\uff921) CO2 using free\uffe2\uff80\uff90air CO2 enrichment (FACE), in low N (unamended field soil) or high N (field soil +4\uffc2\uffa0g\uffc2\uffa0N\uffc2\uffa0m\uffe2\uff88\uff922 years\uffe2\uff88\uff921) treatments.
\uffe2\uff80\uff82There were no CO2\uffc2\uffa0\uffc3\uff97\uffc2\uffa0N interactions. Functional groups responded differently to CO2 and N in terms of biomass, tissue N concentration and soil solution N. Under elevated CO2, forbs, legumes and C3 grasses increased total biomass by 31%, 18%, and 9%, respectively, whereas biomass was reduced in C4\uffe2\uff80\uff90grass monocultures. Two of the four legume species increased biomass and total plant N pools under elevated CO2, probably due to stimulated N\uffe2\uff80\uff90fixation. Only one species markedly shifted the proportional distribution of below\uffe2\uff80\uff90 vs aboveground biomass in response to CO2 or N.
\uffe2\uff80\uff82Although functional groups varied in responses to CO2 and N, there was also substantial variation in responses among species within groups. These results suggest that current trait\uffe2\uff80\uff90based functional classifications might be useful, but not sufficient, for understanding plant and ecosystem responses to elevated CO2 and N availability.
", "keywords": ["580", "N availability", "2. Zero hunger", "0106 biological sciences", "570", "biomass", "legumes", "070601 - Horticultural Crop Growth and Development", "elevated carbon dioxide", "carbon dioxide", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "nitrogen", "forbs", "grasses", "Natural Resources and Conservation", "Functional groups", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://doi.org/10.1046/j.1469-8137.2001.00114.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1046/j.1469-8137.2001.00114.x", "name": "item", "description": "10.1046/j.1469-8137.2001.00114.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1046/j.1469-8137.2001.00114.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2001-05-01T00:00:00Z"}}, {"id": "10.1111/gcb.70084", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:19:26Z", "type": "Journal Article", "created": "2025-03-11", "title": "Higher Plant Diversity Does Not Moderate the Influence of Changing Rainfall Regimes on Plant\u2013Soil Feedback of a Semi\u2010Arid Grassland", "description": "ABSTRACTClimate change is expected to increase the frequency of severe droughts, but it remains unclear whether soil biotic conditioning by plant communities with varying species richness or functional group diversity moderate plant\uffe2\uff80\uff93soil feedback (PSF)\uffe2\uff80\uff94an important ecosystem process driving plant community dynamics\uffe2\uff80\uff94under altered rainfall regimes. We conducted a two\uffe2\uff80\uff90phase PSF experiment to test how plant diversity affects biotic PSF under different rainfall regimes. In Phase 1, we set up mesocosms with 15 plant assemblages composed of two grasses, two forbs and two nitrogen\uffe2\uff80\uff90fixing legumes [one, two, three, or six species from one, two, or three functional group(s)] common to the semi\uffe2\uff80\uff90arid eastern Eurasian Steppe. Mesocosms were subjected to two rainfall amounts (ambient, 50% reduction) crossed with two frequencies (ambient, 50% reduction) for a growing season (~3\uffe2\uff80\uff89months). Conditioned soil from each mesocosm was then used in Phase 2 to inoculate (7% v/v) sterilised mesocosms planted with the same species as in Phase 1 and grown for 8\uffe2\uff80\uff89weeks. Simultaneously, the same plant assemblages were grown in sterilised soil to calculate PSF based on plant biomass measured at the end of Phase 2. Feedback effects differed amongst plant assemblages, but were not significantly altered by reduced rainfall treatments within any plant assemblage. This suggests that the examined interactions between plant and soil microbial communities were resistant to simulated rainfall reductions and that increasing plant diversity did not moderate PSF under altered rainfall regimes. Moreover, increasing plant species richness or functional group diversity did not lessen the magnitude of PSF differences between ambient and reduced rainfall treatments. Collectively, these findings advance our understanding of plant diversity's potential to mitigate climate change effects on PSF, showing that in semi\uffe2\uff80\uff90arid grasslands, higher plant diversity may not moderate PSF responses to altered rainfall regimes and highlighting the importance of considering species\uffe2\uff80\uff90specific traits and interaction stability.Determining underlying physiological patterns governing plant productivity and diversity in grasslands are critical to evaluate species responses to future environmental conditions of elevated CO2 and nitrogen (N) deposition. In a 9\uffe2\uff80\uff90year experiment, N was added to monocultures of seven C3 grassland species exposed to elevated atmospheric CO2 (560\uffe2\uff80\uff83\uffce\uffbcmol\uffe2\uff80\uff83CO2\uffe2\uff80\uff83mol\uffe2\uff88\uff921) to evaluate how N addition affects CO2 responsiveness in species of contrasting functional groups. Functional groups differed in their responses to elevated CO2 and N treatments. Forb species exhibited strong down\uffe2\uff80\uff90regulation of leaf Nmass concentrations (\uffe2\uff88\uff9226%) and photosynthetic capacity (\uffe2\uff88\uff9228%) in response to elevated CO2, especially at high N supply, whereas C3 grasses did not. Hence, achieved photosynthetic performance was markedly enhanced for C3 grasses (+68%) in elevated CO2, but not significantly for forbs. Differences in access to soil resources between forbs and grasses may distinguish their responses to elevated CO2 and N addition. Forbs had lesser root biomass, a lower distribution of biomass to roots, and lower specific root length than grasses. Maintenance of leaf N, possibly through increased root foraging in this nutrient\uffe2\uff80\uff90poor grassland, was necessary to sustain stimulation of photosynthesis under long\uffe2\uff80\uff90term elevated CO2. Dilution of leaf N and associated photosynthetic down\uffe2\uff80\uff90regulation in forbs under elevated [CO2], relative to the C3 grasses, illustrates the potential for shifts in species composition and diversity in grassland ecosystems that have significant forb and grass components.
", "keywords": ["0106 biological sciences", "Nitrogen", "Science", "Ecology and Evolutionary Biology", "Poaceae C3 grass species", "carbon dioxide enrichment", "01 natural sciences", "nitrogen", "C 3 Grass Species", "FACE", "carbon cycle", "Species Functional Groups", "nitrogen cycle", "Free-air CO 2", "Carboxylation Rate", "Photosynthesis", "2. Zero hunger", "photosynthesis", "species diversity", "Geology and Earth Sciences", "carbon dioxide", "Carboxylation rate", "15. Life on land", "Species functional groups", "grasses", "Free-air CO2", "Keywords: angiosperm", "grassland"]}, "links": [{"href": "https://openresearch-repository.anu.edu.au/bitstream/1885/63064/5/Crous-etal_GCB2009-doi-online.pdf.jpg"}, {"href": "https://openresearch-repository.anu.edu.au/bitstream/1885/63064/7/01_Crous_Maintenance_of_leaf_N_controls_2009.pdf.jpg"}, {"href": "https://doi.org/10.1111/j.1365-2486.2009.02058.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2009.02058.x", "name": "item", "description": "10.1111/j.1365-2486.2009.02058.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2009.02058.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-06-01T00:00:00Z"}}, {"id": "10.5281/zenodo.14917866", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:23:53Z", "type": "Dataset", "title": "Data for the manuscript 'Smart mixture design can steer the fate of root derived carbon into mineral-associated and particulate organic matter in intensively managed grasslands'", "description": "To determine the fate of root-derived carbon (C) input from grassland mixtures into distinct soil organic carbon pools \u2014 particulate organic matter (POC) and mineral-associated organic carbon (MAOC) \u2014 a field trial was established, comparing pure stands of perennial ryegrass under high and low nitrogen (N) fertilizer application rates with grassland mixtures containing legumes and forbs at increasing levels of species richness. The mixtures received the low N application rate. Through multiple-pulse \u00b9\u00b3C-CO\u2082 labeling during the first growing season (2022), we captured the net formation of MAOC and POC. At the end of the growing season, soil cores with a diameter of 30 cm were excavated to a depth of 25 cm (topsoil) and transferred to the lab. We removed roots from the soil and performed particle-size fractionation to trace fresh organic carbon (net rhizodeposited C) into particulate organic matter (POM) and mineral-associated organic matter (MAOM).\u00a0Using a cut-off of 50 \u03bcm, particles larger than 50 \u03bcm were classified as POM, while those smaller than 50 \u03bcm were classified as MAOM. We related these soil C fractions to five morphological root traits, as well as to the lignocellulose index and the C:N ratio in root biomass.", "keywords": ["13C isotopic labelling", "Soil carbon fractionation", "Soil carbon storage", "Root traits", "Plant Functional groups", "Legumes", "Forbs"]}, "links": [{"href": "https://doi.org/10.5281/zenodo.14917866"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.14917866", "name": "item", "description": "10.5281/zenodo.14917866", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.14917866"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-02-24T00:00:00Z"}}, {"id": "10067/1920350151162165141", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:25:42Z", "type": "Journal Article", "created": "2022-10-31", "title": "Tree species traits and mycorrhizal association shape soil microbial communities via litter quality and species mediated soil properties", "description": "Open AccessLes sols abritent une grande diversit\u00e9 de microbiote du sol, qui jouent un r\u00f4le crucial dans les processus \u00e9cosyst\u00e9miques cl\u00e9s tels que la transformation de la liti\u00e8re et la min\u00e9ralisation, mais la fa\u00e7on dont les interactions complexes plante-sol fa\u00e7onnent la diversit\u00e9 et la composition du microbiote du sol reste insaisissable. Nous avons effectu\u00e9 le s\u00e9quen\u00e7age de l'amplicon de l'ADN isol\u00e9 \u00e0 partir de la couche arable min\u00e9rale de six arbres europ\u00e9ens communs plant\u00e9s dans des peuplements de monoculture de jardins communs multi-sites d'\u00e9rables \u00e0 feuilles larges et de fr\u00eanes associ\u00e9s \u00e0 des mycorhizes arbusculaires (MA), de h\u00eatres \u00e0 feuilles larges, de chaux et de ch\u00eanes associ\u00e9s \u00e0 des champignons ectomycorhiziens (MCE) et d'\u00e9pinettes de conif\u00e8res associ\u00e9es \u00e0 la MCE. L'objectif principal de cette \u00e9tude \u00e9tait d'\u00e9valuer les effets de l'identit\u00e9 des esp\u00e8ces d'arbres, des traits et des associations mycorhiziennes sur la diversit\u00e9, la structure de la communaut\u00e9, la coh\u00e9sion et le changement dans l'abondance relative des groupes taxonomiques et fonctionnels de bact\u00e9ries, de champignons et de n\u00e9matodes du sol. Nos r\u00e9sultats ont r\u00e9v\u00e9l\u00e9 que les sols sous les feuillus abritaient une plus grande richesse en bact\u00e9ries, champignons et n\u00e9matodes que sous l'\u00e9pinette de Norv\u00e8ge. Les esp\u00e8ces d'arbres \u00e0 feuilles larges associ\u00e9es aux champignons de la MA ont montr\u00e9 une plus grande coh\u00e9sion des communaut\u00e9s bact\u00e9riennes et fongiques que les arbres \u00e0 feuilles larges associ\u00e9s aux champignons de la mec, mais la coh\u00e9sion des communaut\u00e9s de n\u00e9matodes \u00e9tait plus \u00e9lev\u00e9e sous les arbres associ\u00e9s aux champignons de la mec que sous les arbres associ\u00e9s aux champignons de la MA. Les bact\u00e9ries copiotrophes, les saprotrophes fongiques et les n\u00e9matodes bact\u00e9rivores \u00e9taient associ\u00e9s au fr\u00eane, \u00e0 l'\u00e9rable et \u00e0 la chaux ayant un pH du sol \u00e9lev\u00e9 et des indices de d\u00e9composition de la liti\u00e8re \u00e9lev\u00e9s, tandis que les bact\u00e9ries oligotrophes, les champignons ectomycorhiziens et les n\u00e9matodes fongivores \u00e9taient associ\u00e9s au h\u00eatre, au ch\u00eane et \u00e0 l'\u00e9pinette de Norv\u00e8ge qui avaient un pH du sol faible et des indices de d\u00e9composition de la liti\u00e8re faibles. Les esp\u00e8ces d'arbres associ\u00e9es aux champignons AM pr\u00e9sentaient une forte proportion de bact\u00e9ries copiotrophes et de champignons saprotrophes, tandis que les arbres associ\u00e9s aux champignons ECM pr\u00e9sentaient une abondance relative \u00e9lev\u00e9e de bact\u00e9ries oligotrophes, de champignons ECM et de n\u00e9matodes fongivores. Les diff\u00e9rentes abondances de ces groupes fonctionnels soutiennent l'\u00e9conomie nutritive plus inorganique des esp\u00e8ces d'arbres AM par rapport \u00e0 l'\u00e9conomie nutritive plus organique des esp\u00e8ces d'arbres ECM. La communaut\u00e9 bact\u00e9rienne a \u00e9t\u00e9 indirectement affect\u00e9e par la qualit\u00e9 de la liti\u00e8re via les propri\u00e9t\u00e9s du sol, tandis que la communaut\u00e9 fongique a \u00e9t\u00e9 directement affect\u00e9e par la qualit\u00e9 de la liti\u00e8re et les esp\u00e8ces d'arbres. Les groupes fonctionnels des n\u00e9matodes refl\u00e9taient les communaut\u00e9s de bact\u00e9ries et de champignons, indiquant ainsi les groupes principaux et actifs des communaut\u00e9s microbiennes sp\u00e9cifiques aux esp\u00e8ces d'arbres. Notre \u00e9tude a sugg\u00e9r\u00e9 que l'identit\u00e9, les traits et l'association mycorhizienne des esp\u00e8ces d'arbres fa\u00e7onnent consid\u00e9rablement les communaut\u00e9s microbiennes via un effet direct de la chimie de la liti\u00e8re ainsi que via les propri\u00e9t\u00e9s du sol m\u00e9di\u00e9es par la liti\u00e8re.", "keywords": ["Fagus sylvatica", "Soil Science", "Plant Science", "Plant litter", "Agricultural and Biological Sciences", "Soil biology", "Mycorrhizal Fungi and Plant Interactions", "Soil water", "Genetics", "Saproxylic Insect Ecology and Forest Management", "Soil microbiota", "Symbiosis", "Plant Interactions", "Biology", "Ecosystem", "Amplicon sequencing", "Beech", "Ecology", "Bacteria", "Common garden experiment", "Botany", "Life Sciences", "04 agricultural and veterinary sciences", "15. Life on land", "Ectomycorrhiza", "Insect Science", "FOS: Biological sciences", "Functional groups", "Community cohesion", "0401 agriculture", " forestry", " and fisheries", "Trophic interactions", "Soil Carbon Dynamics and Nutrient Cycling in Ecosystems", "Mycorrhiza"]}, "links": [{"href": "https://doi.org/10067/1920350151162165141"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Forest%20Ecology%20and%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10067/1920350151162165141", "name": "item", "description": "10067/1920350151162165141", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10067/1920350151162165141"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-01T00:00:00Z"}}, {"id": "PMC11877630", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-30T16:29:48Z", "type": "Journal Article", "created": "2025-03-11", "title": "Higher Plant Diversity Does Not Moderate the Influence of Changing Rainfall Regimes on Plant\u2013Soil Feedback of a Semi\u2010Arid Grassland", "description": "ABSTRACTClimate change is expected to increase the frequency of severe droughts, but it remains unclear whether soil biotic conditioning by plant communities with varying species richness or functional group diversity moderate plant\uffe2\uff80\uff93soil feedback (PSF)\uffe2\uff80\uff94an important ecosystem process driving plant community dynamics\uffe2\uff80\uff94under altered rainfall regimes. We conducted a two\uffe2\uff80\uff90phase PSF experiment to test how plant diversity affects biotic PSF under different rainfall regimes. In Phase 1, we set up mesocosms with 15 plant assemblages composed of two grasses, two forbs and two nitrogen\uffe2\uff80\uff90fixing legumes [one, two, three, or six species from one, two, or three functional group(s)] common to the semi\uffe2\uff80\uff90arid eastern Eurasian Steppe. Mesocosms were subjected to two rainfall amounts (ambient, 50% reduction) crossed with two frequencies (ambient, 50% reduction) for a growing season (~3\uffe2\uff80\uff89months). Conditioned soil from each mesocosm was then used in Phase 2 to inoculate (7% v/v) sterilised mesocosms planted with the same species as in Phase 1 and grown for 8\uffe2\uff80\uff89weeks. Simultaneously, the same plant assemblages were grown in sterilised soil to calculate PSF based on plant biomass measured at the end of Phase 2. Feedback effects differed amongst plant assemblages, but were not significantly altered by reduced rainfall treatments within any plant assemblage. This suggests that the examined interactions between plant and soil microbial communities were resistant to simulated rainfall reductions and that increasing plant diversity did not moderate PSF under altered rainfall regimes. Moreover, increasing plant species richness or functional group diversity did not lessen the magnitude of PSF differences between ambient and reduced rainfall treatments. Collectively, these findings advance our understanding of plant diversity's potential to mitigate climate change effects on PSF, showing that in semi\uffe2\uff80\uff90arid grasslands, higher plant diversity may not moderate PSF responses to altered rainfall regimes and highlighting the importance of considering species\uffe2\uff80\uff90specific traits and interaction stability.