{"type": "FeatureCollection", "features": [{"id": "10.1016/j.scitotenv.2023.165179", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:17:15Z", "type": "Journal Article", "created": "2023-06-28", "title": "Intensive vegetable production under plastic mulch: A field study on soil plastic and pesticide residues and their effects on the soil microbiome", "description": "Intensive agriculture relies on external inputs to reach high productivity and profitability. Plastic mulch, mainly in the form of Low-Density Polyethylene (LDPE), is widely used in agriculture to decrease evaporation, increase soil temperature and prevent weeds. The incomplete removal of LDPE mulch after use causes plastic contamination in agricultural soils. In conventional agriculture, the use of pesticides also leaves residues accumulating in soils. Thus, the objective of this study was to measure plastic and pesticide residues in agricultural soils and their effects on the soil microbiome. For this, we sampled soil (0-10\u00a0cm and 10-30\u00a0cm) from 18 parcels from 6 vegetable farms in SE Spain. The farms were under either organic or conventional management, where plastic mulch had been used for >25\u00a0years. We measured the macro- and micro-light density plastic debris contents, the pesticide residue levels, and a range of physiochemical properties. We also carried out DNA sequencing on the soil fungal and bacterial communities. Plastic debris (>100\u00a0\u03bcm) was found in all samples with an average number of 2\u00a0\u00d7\u00a0103\u00a0particles\u00a0kg-1 and area of 60\u00a0cm2\u00a0kg-1. We found 4-10 different pesticide residues in all conventional soils, for an average of 140\u00a0\u03bcg\u00a0kg-1. Overall, pesticide content was \u223c100 times lower in organic farms. The soil microbiomes were farm-specific and related to different soil physicochemical parameters and contaminants. Regarding contaminants, bacterial communities responded to the total pesticide residues, the fungicide Azoxystrobin and the insecticide Chlorantraniliprole as well as the plastic area. The fungicide Boscalid was the only contaminant to influence the fungal community. The wide spread of plastic and pesticide residues in agricultural soil and their effects on soil microbial communities may impact crop production and other environmental services. More studies are required to evaluate the total costs of intensive agriculture.", "keywords": ["2. Zero hunger", "Plastic mulch", "Soil microbiome", "Microbiota", "Microplastic", "Pesticide Residues", "Pesticides residues", "Agriculture", "12. Garantizar modalidades de consumo y producci\u00f3n sostenibles", "15. Life on land", "Fungicides", " Industrial", "Edafolog\u00eda y Qu\u00edmica Agr\u00edcola", "Soil", "Intensive vegetable production", "13. Climate action", "Agriculture contamination", "Polyethylene", "Vegetables", "31 Ciencias Agrarias::3101 Agroqu\u00edmica", "Pesticides"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2023.165179"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2023.165179", "name": "item", "description": "10.1016/j.scitotenv.2023.165179", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2023.165179"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}, {"id": "10.1007/s00128-012-0523-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:14:39Z", "type": "Journal Article", "created": "2012-01-25", "title": "Effect Of Long-Term Zinc Pollution On Soil Microbial Community Resistance To Repeated Contamination", "description": "The aim of the study was to compare the effects of stress (contamination trials) on the microorganisms in zinc-polluted soil (5,018\u00a0mg Zn\u00a0kg(-1) soil dry weight) and unpolluted soil (141\u00a0mg Zn kg(-1) soil\u00a0dw), measured as soil respiration rate. In the laboratory, soils were subjected to copper contamination (0, 500, 1,500 and 4,500\u00a0mg\u00a0kg(-1) soil\u00a0dw), and then a bactericide (oxytetracycline) combined with a fungicide (captan) along with glucose (10\u00a0mg\u00a0g(-1) soil\u00a0dw each) were added. There was a highly significant effect of soil type, copper treatment and oxytetracycline/captan treatment. The initial respiration rate of chronically zinc-polluted soil was higher than that of unpolluted soil, but in the copper treatment it showed a greater decline. Microorganisms in copper-treated soil were more susceptible to oxytetracycline/captan contamination. After the successive soil contamination trials the decline of soil respiration was greater in zinc-polluted soil than in unpolluted soil.", "keywords": ["Health", " Toxicology and Mutagenesis", "trace metals", "Oxytetracycline", "Toxicology", "01 natural sciences", "Article", "Captan", "Soil", "Stress", " Physiological", "Soil Pollutants", "Soil Microbiology", "combined stressors", "0105 earth and related environmental sciences", "soil pollution", "Drug Resistance", " Microbial", "04 agricultural and veterinary sciences", "Pollution", "Adaptation", " Physiological", "soil respiration rate", "6. Clean water", "Anti-Bacterial Agents", "Fungicides", " Industrial", "Zinc", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Copper"], "contacts": [{"organization": "Klimek, Beata", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1007/s00128-012-0523-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bulletin%20of%20Environmental%20Contamination%20and%20Toxicology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00128-012-0523-0", "name": "item", "description": "10.1007/s00128-012-0523-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00128-012-0523-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-01-26T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2024.134231", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:16:59Z", "type": "Journal Article", "created": "2024-04-06", "title": "Soil microbial community fragmentation reveals indirect effects of fungicide exposure mediated by biotic interactions between microorganisms", "description": "Fungicides are used worldwide to improve crop yields, but they can affect non-target soil microorganisms which are essential for ecosystem functioning. Microorganisms form complex communities characterized by a myriad of interspecies interactions, yet it remains unclear to what extent non-target microorganisms are indirectly affected by fungicides through biotic interactions with sensitive taxa. To quantify such indirect effects, we fragmented a soil microbial community by filtration to alter biotic interactions and compared the effect of the fungicide hymexazol between fractions in soil microcosms. We postulated that OTUs which are indirectly affected would exhibit a different response to the fungicide across the fragmented communities. We found that hymexazol primarily affected bacterial and fungal communities through indirect effects, which were responsible for more than 75% of the shifts in relative abundance of the dominant microbial OTUs after exposure to an agronomic dose of hymexazol. However, these indirect effects decreased for the bacterial community when hymexazol doses increased. Our results also suggest that N-cycling processes such as ammonia oxidation can be impacted indirectly by fungicide application. This work sheds light on the indirect impact of fungicide exposure on soil microorganisms through biotic interactions, which underscores the need for higher-tier risk assessment. ENVIRONMENTAL IMPLICATION: In this study, we used a novel approach based on the fragmentation of the soil microbial community to determine to which extent fungicide application could indirectly affect fungi and bacteria through biotic interactions. To assess off-target effects of fungicide on soil microorganisms, we selected hymexazol, which is used worldwide to control a variety of fungal plant pathogens, and exposed arable soil to the recommended field rate, as well as to higher rates. Our findings show that at least 75% of hymexazol-impacted microbial OTUs were indirectly affected, therefore emphasizing the importance of tiered risk assessment.", "keywords": ["2. Zero hunger", "570", "Bacteria", "hymexazol", "[SDV]Life Sciences [q-bio]", "Microbiota", "Fungi", "500", "[SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study", "15. Life on land", "Fungicides", " Industrial", "[SDV] Life Sciences [q-bio]", "nitrogen cycling", "13. Climate action", "network", "ammonia-oxidizing microorganism", "Soil Pollutants", "Microbial Interactions", "[SDV.SA.SDS] Life Sciences [q-bio]/Agricultural sciences/Soil study", "pesticide", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.jhazmat.2024.134231"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2024.134231", "name": "item", "description": "10.1016/j.jhazmat.2024.134231", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2024.134231"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-05-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2013.07.064", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:17:10Z", "type": "Journal Article", "created": "2013-08-19", "title": "Effect Of Long Term Organic Amendments And Vegetation Of Vineyard Soils On The Microscale Distribution And Biogeochemistry Of Copper", "description": "In this study we evaluated the effect of the long term organic management of a vineyard-soil on the biogeochemistry of copper at the micro-aggregate scale. The model vineyard-soil (M\u00e2con-France) experienced a long-term field-experiment that consisted in amendments and vegetations with various materials and plants. We studied specifically the effect of Straw (S) and Conifer Compost (CC) organic amendments and Clover (Cl) and Fescue (F) vegetation on the fate of copper (fungicide) in the surface layer of this loamy soil, through a comparison with the Non Amended soil (NA). After collection the five soils were immediately physically fractionated in order to obtain 5 granulometric size-fractions. All soils and size-fractions were quantitatively characterized in terms of granulometry, chemical content and copper distribution, speciation and bioavailability to bacteria and plants. The results showed strong increases of soil-constituents aggregation for all treatments (Cl>CC>S>F>NA), in relation with the increased cementation of soil-constituents by organic matter (OM). The distribution patterns of all major elements and organic carbon were found highly variable within the soil sub-fractions and also between the 5 treatments. Due to their specific inorganic and organic composition, soil sub-fractions can thus be considered as a specific microbial habitat. Added OM accumulated preferentially in the 20-2 \u03bcm and in the >250 \u03bcm of the 5 soils. The distribution patterns of copper as well as its speciation and bioavailability to bacteria in the soil sub-fractions were shown to be strongly different among the five soils, in relation with OM distribution. Our results also suggest that Cu-bioavailability to plants is controlled by soil-rhizosphere structure. Altogether our results permitted to show that long-term organic management of a vineyard soil induced stable modifications of soil physical and chemical properties at both macro and micro-scales. These modifications affected in turn the micro-scale biogeochemistry of copper, and especially its bioavailability to bacteria and plants.", "keywords": ["2. Zero hunger", "Organic Agriculture", "Bacteria", "Spectrophotometry", " Atomic", "Biological Availability", "04 agricultural and veterinary sciences", "Plants", "15. Life on land", "01 natural sciences", "Fungicides", " Industrial", "Soil", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "France", "Seasons", "Copper", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2013.07.064"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2013.07.064", "name": "item", "description": "10.1016/j.scitotenv.2013.07.064", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2013.07.064"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-01-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2024.177557", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:17:16Z", "type": "Journal Article", "created": "2024-11-20", "title": "Dynamic response of soil microbial communities and network to hymexazol exposure", "description": "Fungicides are an essential component of current agricultural practices, but their extensive use has raised concerns about their effects on non-target soil microorganisms, which carry out essential ecosystem functions. However, despite the complexity of microbial communities, many studies investigating their response to fungicides focus only on bacteria or fungi at one point in time. In this study, we used amplicon sequencing to assess the effect of the fungicide hymexazol on the diversity, composition, and co-occurrence network of soil bacteria, fungi, and protists at 7, 21, and 60\u00a0days after application. We found that hymexazol had very little effect on microbial alpha-diversity, but that microbial community composition and OTU differential abundance were altered over the duration of the experiment, even after hymexazol concentrations were undetectable. The co-occurrence patterns within and between microbial kingdoms were affected by hymexazol dose, suggesting that indirect effects may play a role in the microbial community response. Nitrogen cycling was also affected, with a transient hymexazol-associated increase in the abundance of ammonia-oxidizing microorganisms and soil nitrate concentration. These findings highlight that the effects of fungicides on soil microorganisms are dynamic and extensive, spanning several taxonomic kingdoms.", "keywords": ["570", "Bacteria", "Fungicide", "Microbiota", "Fungi", "Protists", "[SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study", "Nitrification", "630", "Fungicides", " Industrial", "Pesticide", "Soil", "Soil Pollutants", "[SDV.SA.SDS] Life Sciences [q-bio]/Agricultural sciences/Soil study", "Soil Microbiology", "Nitrogen cycling"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2024.177557"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2024.177557", "name": "item", "description": "10.1016/j.scitotenv.2024.177557", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2024.177557"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "10.1038/s41598-019-56741-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:18:25Z", "type": "Journal Article", "created": "2020-01-20", "title": "Seasonal epiphytic microbial dynamics on grapevine leaves under biocontrol and copper fungicide treatments", "description": "Abstract<p>Winemakers have long used copper as a conventional fungicide treatment on grapevine and only recently, the use of biocontrol agents depicted a promising alternative. Most of the studies that investigate the impact of fungicide treatments, focus on specific pathogenic microbes. In the present work instead, a field experiment conducted in South Africa shows the seasonal microbial change occurring on grapevine leaves, periodically treated with two different fungicide treatments: copper sulphate and Lactobacillus plantarum MW-1. In this work, NGS data were combined with strain-specific and community qPCRs to reveal the shift of the microbial communities throughout the growing season and highlight the impact of fungicides on the microbiota. Only the family of Lactobacillaceae systematically changed between treatments, while the bacterial community remained relatively stable over time. MW-1 was exclusively detected on biocontrol-sprayed leaves. Conversely, the fungal community was largely shaped by sampling time, underlining the succession of different dominant taxa over the months. Between treatments, only a few fungal taxa appeared to change significantly and the fungal load was also comparable. Monitoring the dynamics of the microbial community under different fungicide treatments may advise the best timing to apply treatments to the plants, toward the realization of more sustainable agricultural practices.</p>", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Copper Sulfate", "Agriculture", "Article", "Fungicides", " Industrial", "3. Good health", "Plant Leaves", "South Africa", "03 medical and health sciences", "Vitis", "Seasons", "Lactobacillus plantarum", "Mycobiome"]}, "links": [{"href": "https://www.nature.com/articles/s41598-019-56741-z.pdf"}, {"href": "https://doi.org/10.1038/s41598-019-56741-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41598-019-56741-z", "name": "item", "description": "10.1038/s41598-019-56741-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41598-019-56741-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-20T00:00:00Z"}}, {"id": "10.14601/phytopathol_mediterr-23515", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:20:17Z", "type": "Journal Article", "title": "Endotherapy of infected grapevine cuttings for the control of Phaeomoniella chlamydospora and Phaeoacremonium minimum", "description": "The pathogens Phaeomoniella chlamydospora and Phaeoacremonium minimum are associated with different syndromes of the esca disease complex affecting grapevine propagation material, and young and adult plants. Infections by these fungi occur in grapevine nurseries and in vineyards, with disease control strategies providing limited protection in both cases. Several chemicals are effective in vitro against these two pathogens, but treatment of infected plants, especially endotherapy, has not yet proven satisfactory. Five chemicals (elemental silver, fosetyl-Al, glutaraldehyde, hydrogen peroxide and Blad-containing oligomer) were tested in vitro, with the first four also tested in planta, by means of endotherapy, against Pa. chlamydospora and Pm. minimum. All chemicals were effective in vitro for preventing growth of both pathogens, at different concentrations. Endotherapy of rooted grapevine cuttings (cv. Touriga Nacional) was effective against Pa. chlamydospora for all the tested chemicals, with reductions in the frequency of re-isolation of this pathogen of 91\u201395% (glutaraldehyde), 68\u201396% (hydrogen peroxide), 68\u201377% (elemental silver) and 58\u201359% (fosetyl-Al) when compared with the water-treated experimental controls. The only treatment that was effective against Pm. minimum was glutaraldehyde, providing a 75\u201383% reduction in re-isolation frequency. These results indicate that endotherapy of young grapevines during early stages of infection may be an effective control strategy, especially against the wood pathogen Pa. chlamydospora.", "keywords": ["0106 biological sciences", "tracheomycosis", "chemical control", "QK1-989", "Botany", "grapevine trunk diseases", "01 natural sciences", "fungicides"]}, "links": [{"href": "https://doi.org/10.14601/phytopathol_mediterr-23515"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Phytopathologia%20Mediterranea", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.14601/phytopathol_mediterr-23515", "name": "item", "description": "10.14601/phytopathol_mediterr-23515", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.14601/phytopathol_mediterr-23515"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-01-01T00:00:00Z"}}, {"id": "13946230", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:26:22Z", "type": "Journal Article", "created": "2013-06-11", "title": "Observations on Mechanism of Action of the Antifungal Peptide, Ro2-7758.", "description": "SummaryInhibitory action of the antifungal sulfur-containing peptide, Ro2-7758 (formerly X-5079C), against the growth of Mucor corymbifera was annulled by sulfite and cysteine, but not by sulfate or 96 other substances tested. Experiments with S35O4 showed that the drug had no effect on permeability of yeast cells to sulfate. However, production of S35O3 from S35O4 by a cell-free sulfate reductase system from yeast was first stimulated at low drug concentrations and then inhibited at higher concentrations. It was concluded that this action of the drug on sulfate reductase may be involved in its antibiotic activity.", "keywords": ["Antifungal Agents", "Mucor", "Sulfites", "Cysteine", "Peptides", "Fungicides", " Industrial", "3. Good health"], "contacts": [{"organization": "G R, GALE, S M, KENDALL, A M, WELCH,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/13946230"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Experimental%20Biology%20and%20Medicine", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "13946230", "name": "item", "description": "13946230", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/13946230"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "1963-05-01T00:00:00Z"}}, {"id": "10317/18590", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:25:59Z", "type": "Journal Article", "created": "2023-06-27", "title": "Intensive vegetable production under plastic mulch: A field study on soil plastic and pesticide residues and their effects on the soil microbiome", "description": "Intensive agriculture relies on external inputs to reach high productivity and profitability. Plastic mulch, mainly in the form of Low-Density Polyethylene (LDPE), is widely used in agriculture to decrease evaporation, increase soil temperature and prevent weeds. The incomplete removal of LDPE mulch after use causes plastic contamination in agricultural soils. In conventional agriculture, the use of pesticides also leaves residues accumulating in soils. Thus, the objective of this study was to measure plastic and pesticide residues in agricultural soils and their effects on the soil microbiome. For this, we sampled soil (0-10\u00a0cm and 10-30\u00a0cm) from 18 parcels from 6 vegetable farms in SE Spain. The farms were under either organic or conventional management, where plastic mulch had been used for >25\u00a0years. We measured the macro- and micro-light density plastic debris contents, the pesticide residue levels, and a range of physiochemical properties. We also carried out DNA sequencing on the soil fungal and bacterial communities. Plastic debris (>100\u00a0\u03bcm) was found in all samples with an average number of 2\u00a0\u00d7\u00a0103\u00a0particles\u00a0kg-1 and area of 60\u00a0cm2\u00a0kg-1. We found 4-10 different pesticide residues in all conventional soils, for an average of 140\u00a0\u03bcg\u00a0kg-1. Overall, pesticide content was \u223c100 times lower in organic farms. The soil microbiomes were farm-specific and related to different soil physicochemical parameters and contaminants. Regarding contaminants, bacterial communities responded to the total pesticide residues, the fungicide Azoxystrobin and the insecticide Chlorantraniliprole as well as the plastic area. The fungicide Boscalid was the only contaminant to influence the fungal community. The wide spread of plastic and pesticide residues in agricultural soil and their effects on soil microbial communities may impact crop production and other environmental services. More studies are required to evaluate the total costs of intensive agriculture.", "keywords": ["2. Zero hunger", "Plastic mulch", "Soil microbiome", "Microbiota", "Microplastic", "Pesticide Residues", "Pesticides residues", "Agriculture", "12. Garantizar modalidades de consumo y producci\u00f3n sostenibles", "15. Life on land", "Fungicides", " Industrial", "Edafolog\u00eda y Qu\u00edmica Agr\u00edcola", "Soil", "Intensive vegetable production", "13. Climate action", "Agriculture contamination", "Polyethylene", "Vegetables", "31 Ciencias Agrarias::3101 Agroqu\u00edmica", "Pesticides"]}, "links": [{"href": "https://doi.org/10317/18590"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10317/18590", "name": "item", "description": "10317/18590", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10317/18590"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}, {"id": "38598881", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:27:59Z", "type": "Journal Article", "created": "2024-04-06", "title": "Soil microbial community fragmentation reveals indirect effects of fungicide exposure mediated by biotic interactions between microorganisms", "description": "Fungicides are used worldwide to improve crop yields, but they can affect non-target soil microorganisms which are essential for ecosystem functioning. Microorganisms form complex communities characterized by a myriad of interspecies interactions, yet it remains unclear to what extent non-target microorganisms are indirectly affected by fungicides through biotic interactions with sensitive taxa. To quantify such indirect effects, we fragmented a soil microbial community by filtration to alter biotic interactions and compared the effect of the fungicide hymexazol between fractions in soil microcosms. We postulated that OTUs which are indirectly affected would exhibit a different response to the fungicide across the fragmented communities. We found that hymexazol primarily affected bacterial and fungal communities through indirect effects, which were responsible for more than 75% of the shifts in relative abundance of the dominant microbial OTUs after exposure to an agronomic dose of hymexazol. However, these indirect effects decreased for the bacterial community when hymexazol doses increased. Our results also suggest that N-cycling processes such as ammonia oxidation can be impacted indirectly by fungicide application. This work sheds light on the indirect impact of fungicide exposure on soil microorganisms through biotic interactions, which underscores the need for higher-tier risk assessment. ENVIRONMENTAL IMPLICATION: In this study, we used a novel approach based on the fragmentation of the soil microbial community to determine to which extent fungicide application could indirectly affect fungi and bacteria through biotic interactions. To assess off-target effects of fungicide on soil microorganisms, we selected hymexazol, which is used worldwide to control a variety of fungal plant pathogens, and exposed arable soil to the recommended field rate, as well as to higher rates. Our findings show that at least 75% of hymexazol-impacted microbial OTUs were indirectly affected, therefore emphasizing the importance of tiered risk assessment.", "keywords": ["2. Zero hunger", "570", "Bacteria", "hymexazol", "[SDV]Life Sciences [q-bio]", "Microbiota", "Fungi", "500", "[SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study", "15. Life on land", "Fungicides", " Industrial", "[SDV] Life Sciences [q-bio]", "nitrogen cycling", "13. Climate action", "network", "ammonia-oxidizing microorganism", "Soil Pollutants", "Microbial Interactions", "[SDV.SA.SDS] Life Sciences [q-bio]/Agricultural sciences/Soil study", "pesticide", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/38598881"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "38598881", "name": "item", "description": "38598881", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/38598881"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-05-01T00:00:00Z"}}, {"id": "39557169", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:28:01Z", "type": "Journal Article", "created": "2024-11-20", "title": "Dynamic response of soil microbial communities and network to hymexazol exposure", "description": "Fungicides are an essential component of current agricultural practices, but their extensive use has raised concerns about their effects on non-target soil microorganisms, which carry out essential ecosystem functions. However, despite the complexity of microbial communities, many studies investigating their response to fungicides focus only on bacteria or fungi at one point in time. In this study, we used amplicon sequencing to assess the effect of the fungicide hymexazol on the diversity, composition, and co-occurrence network of soil bacteria, fungi, and protists at 7, 21, and 60\u00a0days after application. We found that hymexazol had very little effect on microbial alpha-diversity, but that microbial community composition and OTU differential abundance were altered over the duration of the experiment, even after hymexazol concentrations were undetectable. The co-occurrence patterns within and between microbial kingdoms were affected by hymexazol dose, suggesting that indirect effects may play a role in the microbial community response. Nitrogen cycling was also affected, with a transient hymexazol-associated increase in the abundance of ammonia-oxidizing microorganisms and soil nitrate concentration. These findings highlight that the effects of fungicides on soil microorganisms are dynamic and extensive, spanning several taxonomic kingdoms.", "keywords": ["570", "Bacteria", "Fungicide", "Microbiota", "Fungi", "Protists", "[SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study", "Nitrification", "630", "Fungicides", " Industrial", "Pesticide", "Soil", "Soil Pollutants", "[SDV.SA.SDS] Life Sciences [q-bio]/Agricultural sciences/Soil study", "Soil Microbiology", "Nitrogen cycling"]}, "links": [{"href": "https://doi.org/39557169"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "39557169", "name": "item", "description": "39557169", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/39557169"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "40377710", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-07-27T16:28:06Z", "type": "Journal Article", "created": "2025-01-22", "title": "Development of a High-Throughput Spore Germination Test to Assess the Toxicity of Pesticides on Arbuscular Mycorrhizal Fungi", "description": "Pesticides are essential agricultural inputs that help securing crop yields. However, they can affect non-target soil microorganisms, including arbuscular mycorrhizal (AM) fungi, that are potential indicators of the toxicity of pesticides on the soil microbiota. Here, we developed a fast-track high-throughput spore germination test, for AM fungi produced in vitro. This test allows the determination of EC50 values and the nature of the effects of pesticides on AM fungal spores (fungicidal or fungistatic). First, 19 active ingredients were tested on Rhizophagus intraradices MUCL 49410. Secondly, five of these compounds, varying in their toxicity to R. intraradices, were tested on three additional AM fungi (Rhizophagus irregularis MUCL 41833, Rhizophagus clarus MUCL 46238 and Rhizophagus aggregatus MUCL 49408). Our results showed that the toxicity of pesticides varied according to their chemical nature, concentration and AM fungal species tested. With the exception of 3,5,6-trichloro-2-pyridinol (TCP, a transformation product of chlorpyrifos), insecticides and herbicides had no detrimental effect on spore germination at the concentration expected in soil upon application of the recommended dose, unlike most fungicides, which had an impact on one or more AM fungi. Fludioxonil and pyraclostrobin were by far the most problematic fungicide and R. aggregatus the most sensitive strain to pesticides. This AM fungus could thus be a good indicator to be used in standard ecotoxicity testing. In conclusion, we present a fast-track, high-throughput testing system for assessing the toxicity of pesticides on AM fungi, using spore germination as a relevant endpoint, that could be used as a first-tier screening tool in pesticide risk assessment.", "keywords": ["Mycorrhizae", "Spores", " Fungal", "Pesticides", "Glomeromycota", "Soil Microbiology", "High-Throughput Screening Assays", "Fungicides", " Industrial"]}, "links": [{"href": "https://doi.org/40377710"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Mycorrhiza", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "40377710", "name": "item", "description": "40377710", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/40377710"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Fungicides&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Fungicides&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Fungicides&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Fungicides&offset=12", "hreflang": "en-US"}], "numberMatched": 12, "numberReturned": 12, "distributedFeatures": [], "timeStamp": "2026-07-28T15:19:45.206110Z"}