{"type": "FeatureCollection", "features": [{"id": "10.1016/j.jhazmat.2014.03.017", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-24T16:15:48Z", "type": "Journal Article", "created": "2014-03-22", "title": "A Three-Year Experiment Confirms Continuous Immobilization Of Cadmium And Lead In Contaminated Paddy Field With Biochar Amendment", "description": "Heavy metal contamination in croplands has been a serious concern because of its high health risk through soil-food chain transfer. A field experiment was conducted in 2010-2012 in a contaminated rice paddy in southern China to determine if bioavailability of soil Cd and Pb could be reduced while grain yield was sustained over 3 years after a single soil amendment of wheat straw biochar. Contaminated biochar particles were separated from the biochar amended soil and microscopically analyzed to help determine where, and how, metals were immobilized with biochar. Biochar soil amendment (BSA) consistently and significantly increased soil pH, total organic carbon and decreased soil extractable Cd and Pb over the 3 year period. While rice plant tissues' Cd content was significantly reduced, depending on biochar application rate, reduction in plant Pb concentration was found only in root tissue. Analysis of the fresh and contaminated biochar particles indicated that Cd and Pb had probably been bonded with the mineral phases of Al, Fe and P on and around and inside the contaminated biochar particle. Immobilization of the Pb and Cd also occurred to cation exchange on the porous carbon structure.", "keywords": ["China", "anzsrc-for: 4105 Pollution and Contamination", "Soil remediation", "0211 other engineering and technologies", "4102 Ecological Applications", "Aged biochar", "02 engineering and technology", "41 Environmental Sciences", "01 natural sciences", "630", "anzsrc-for: 41 Environmental Sciences", "4105 Pollution and Contamination", "anzsrc-for: 40 Engineering", "Soil", "anzsrc-for: 34 Chemical sciences", "Metals", " Heavy", "Soil Pollutants", "Biomass", "Organic Chemicals", "anzsrc-for: 03 Chemical Sciences", "0105 earth and related environmental sciences", "2. Zero hunger", "anzsrc-for: 05 Environmental Sciences", "Oryza", "Heavy", "Hydrogen-Ion Concentration", "Heavy metal pollution", "anzsrc-for: 4102 Ecological Applications", "Carbon", "6. Clean water", "Biochar", "Lead", "Metals", "13. Climate action", "Charcoal", "Rice paddy", "Adsorption", "anzsrc-for: 09 Engineering", "Cadmium"]}, "links": [{"href": "https://doi.org/10.1016/j.jhazmat.2014.03.017"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2014.03.017", "name": "item", "description": "10.1016/j.jhazmat.2014.03.017", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2014.03.017"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-05-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2009.08.045", "type": "Feature", "geometry": null, "properties": {"updated": "2026-08-24T16:15:55Z", "type": "Journal Article", "created": "2009-09-23", "title": "Application Of Temporal Temperature Gradient Gel Electrophoresis For Characterisation Of Fungal Endophyte Communities Of Salix Caprea L. In A Heavy Metal Polluted Soil", "description": "Fungal endophytes can affect the heavy metal uptake of their host plants and increase the tolerance of their host plants to heavy metal stress. Therefore, in the present study, a wide-range screening of the fungal endophyte communities was conducted to determine the fungal distribution and diversity on S. caprea roots on a metal polluted site. Fungal communities were screened using amplification with the 5.8S-ITS2-28S part of the rDNA operon, with the resulting amplicons analysed by temporal temperature gradient gel electrophoresis (TTGE) and sequencing. This technique is reproducible and shows good coverage of ascomycete and basidiomycete taxa, as 68% and 32% of all of the sequences, respectively. No clear shift in fungal ITS-TTGE profiles from S. caprea roots was seen along the secondary succession stages. Ascomycetes dominated the more polluted plots, while there was a greater diversity of basidiomycetes in the less polluted and control plots, suggesting greater tolerance of ascomycetes in comparison with basidiomycete fungi. The high diversity of DSEs was confirmed at the highly metal-enriched locations, with species belonging to the genera Phialophora, Phialocephala and Leptodontidium. Furthermore, the DSE colonisation of S. caprea roots and the frequency of the sequences showing affinity towards DSE genus Phialophora, showed good correspondence with soil Pb, Cd and plant-available P concentrations, possibly indicating that DSEs improve metal tolerance of willows to high heavy metal contamination.", "keywords": ["Electrophoresis", " Agar Gel", "0301 basic medicine", "dark septate endophytes", "Fungi", "Temperature", "mycorrhiza", "Salix", "04 agricultural and veterinary sciences", "info:eu-repo/classification/udc/581", "15. 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However, the symbiotic functions of AMF in remediation of polluted soils depend on plant\u2013fungus\u2013soil combinations and are greatly influenced by environmental conditions. To better understand the adaptation of plants and the related mycorrhizae to extreme environmental conditions, AMF colonization, spore density and community structure were analyzed in roots or rhizosphere soils of Robinia pseudoacacia. Mycorrhization was compared between uncontaminated soil and heavy metal contaminated soil from a lead\u2013zinc mining region of northwest China. Samples were analyzed by restriction fragment length polymorphism (RFLP) screening with AMF-specific primers (NS31 and AM1), and sequencing of rRNA small subunit (SSU). The phylogenetic analysis revealed 28 AMF group types, including six AMF families: Glomeraceae, Claroideoglomeraceae, Diversisporaceae, Acaulosporaceae, Pacisporaceae, and Gigasporaceae. Of all AMF group types, six (21%) were detected based on spore samples alone, four (14%) based on root samples alone, and five (18%) based on samples from root, soil and spore. Glo9 (Rhizophagus intraradices), Glo17 (Funneliformis mosseae) and Acau3 (Acaulospora sp.) were the three most abundant AMF group types in the current study. Soil Pb and Zn concentrations, pH, organic matter content, and phosphorus levels all showed significant correlations with the AMF species compositions in root and soil samples. Overall, the uncontaminated sites had higher species diversity than sites with heavy metal contamination. The study highlights the effects of different soil chemical parameters on AMF colonization, spore density and community structure in contaminated and uncontaminated sites. The tolerant AMF species isolated and identified from this study have potential for application in phytoremediation of heavy metal contaminated areas.", "keywords": ["2. 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