{"type": "FeatureCollection", "features": [{"id": "10.1007/s00484-016-1135-x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:15:23Z", "type": "Journal Article", "created": "2016-02-06", "title": "Influence of ground surface characteristics on the mean radiant temperature in urban areas", "description": "The effect of variations in land cover on mean radiant temperature (T mrt ) is explored through a simple scheme developed within the radiation model SOLWEIG. Outgoing longwave radiation is parameterised using surface temperature observations on a grass and an asphalt surface, whereas outgoing shortwave radiation is modelled through variations in albedo for the different surfaces. The influence of ground surface materials on T mrt is small compared to the effects of shadowing. Nevertheless, altering ground surface materials could contribute to a reduction in T mrt to reduce the radiant load during heat-wave episodes in locations where shadowing is not an option. Evaluation of the new scheme suggests that despite its simplicity it can simulate the outgoing fluxes well, especially during sunny conditions. However, it underestimates at night and in shadowed locations. One grass surface used to develop the parameterisation, with very different characteristics compared to an evaluation grass site, caused T mrt to be underestimated. The implications of using high temporal resolution (e.g. 15 minutes) meteorological forcing data under partly cloudy conditions are demonstrated even for fairly proximal sites.", "keywords": ["13. Climate action", "London", "11. Sustainability", "Sunlight", "Temperature", "Water", "Models", " Theoretical", "15. Life on land", "Poaceae", "01 natural sciences", "Hydrocarbons", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://centaur.reading.ac.uk/58036/1/IJB_Lindberg_2016.pdf"}, {"href": "http://link.springer.com/content/pdf/10.1007/s00484-016-1135-x"}, {"href": "https://doi.org/10.1007/s00484-016-1135-x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Biometeorology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00484-016-1135-x", "name": "item", "description": "10.1007/s00484-016-1135-x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00484-016-1135-x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-02-06T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2023.161600", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:17:29Z", "type": "Journal Article", "created": "2023-01-18", "title": "Risk reductions during pyrene biotransformation and mobilization in a model plant-bacteria-biochar system", "description": "The productive application of motile microorganisms for degrading hydrophobic contaminants in soil is one of the most promising processes in modern remediation due to its sustainability and low cost. However, the incomplete biodegradation of the contaminants and the formation of the intermediary metabolites in the process may increase the toxicity in soil during bioremediation, and motile inoculants may mobilize the pollutants through biosorption. Therefore, controlling these factors should be a fundamental part of soil remediation approaches. The aim of this study was to evaluate the sources of risk associated with the cometabolism-based transformation of 14C-labeled pyrene by inoculated Pseudomonas putida G7 and identify ways to minimize risk. Our model scenario examined the increase in bioaccessibility to a distant source of contamination facilitated by sunflower (Helianthus annuus L.) roots. A biochar trap for mobilized pollutant metabolites and bacteria has also been employed. The experimental design consisted of pots filled with a layer of sand with 14C-labeled pyrene (88 mg kg-1) as a contamination focus located several centimeters from the inoculation point. Half of the pots included a biochar layer at the bottom. The pots were incubated in a greenhouse with sunflower plants and P. putida G7 bacteria. Pots with sunflower plants showed a higher biodegradation of pyrene, its mobilization as metabolites through the percolate and the roots, and bacterial mobilization toward the source of contamination, also resulting in increased pyrene transformation. In addition, the biochar layer efficiently reduced the concentrations of pyrene metabolites collected in the leachates. Therefore, the combination of plants, motile bacteria and biochar safely reduced the risk caused by the biological transformation of pyrene.", "keywords": ["Risk", "2. Zero hunger", "570", "Pyrenes", "Bacteria", "04 agricultural and veterinary sciences", "Plants", "01 natural sciences", "Polycyclic aromatic hydrocarbons", "6. Clean water", "Sunflower", "Soil", "Biodegradation", " Environmental", "13. Climate action", "Biodegradation", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Polycyclic Aromatic Hydrocarbons", "Bioremediation", "Biotransformation", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2023.161600"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2023.161600", "name": "item", "description": "10.1016/j.scitotenv.2023.161600", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2023.161600"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-04-01T00:00:00Z"}}, {"id": "10.1007/s11356-024-32916-8", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:15:55Z", "type": "Journal Article", "created": "2024-03-22", "title": "Bioaugmentation and vermicompost facilitated the hydrocarbon bioremediation: scaling up from lab to field for petroleum-contaminated soils", "description": "Abstract<p>The biodegradation of total petroleum hydrocarbon (TPH) in soil is very challenging due to the complex recalcitrant nature of hydrocarbon, hydrophobicity, indigenous microbial adaptation and competition, and harsh environmental conditions. This work further confirmed that limited natural attenuation of petroleum hydrocarbons (TPHs) (15% removal) necessitates efficient bioremediation strategies. Hence, a scaling-up experiment for testing and optimizing the use of biopiles for bioremediation of TPH polluted soils was conducted with three 500-kg pilots of polluted soil, and respective treatments were implemented: including control soil (CT), bioaugmentation and vermicompost treatment (BAVC), and a combined application of BAVC along with bioelectrochemical snorkels (BESBAVC), all maintained at 40% field capacity. This study identified that at pilot scale level, a successful application of BAVC treatment can achieve 90.3% TPH removal after 90 days. BAVC\uffe2\uff80\uff99s effectiveness stemmed from synergistic mechanisms. Introduced microbial consortia were capable of TPH degradation, while vermicompost provided essential nutrients, enhanced aeration, and, potentially, acted as a biosorbent. Hence, it can be concluded that the combined application of BAVC significantly enhances TPH removal compared to natural attenuation. While the combined application of a bioelectrochemical snorkel (BES) with BAVC also showed a significant TPH removal, it did not differ statistically from the individual application of BAVC, under applied conditions. Further research is needed to optimize BES integration with BAVC for broader applicability. This study demonstrates BAVC as a scalable and mechanistically sound approach for TPH bioremediation in soil.</p", "keywords": ["Qu\u00edmica agr\u00edcola", "Bioqu\u00edmica", "0301 basic medicine", "vermicompost", "Passive bioelectrochemical systems", "Contaminaci\u00f3n", "passive bioelectrochemical systems", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Pollution", "Biochemistry", "01 natural sciences", "Hydrocarbons", "Microbial consortium", "03 medical and health sciences", "Bioaugmentation", "Agricultural chemistry", "microbial consortium", "hydrocarbons", "Vermicompost", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s11356-024-32916-8"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11356-024-32916-8", "name": "item", "description": "10.1007/s11356-024-32916-8", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11356-024-32916-8"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-03-22T00:00:00Z"}}, {"id": "10.1016/j.electacta.2021.138636", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:16:44Z", "type": "Journal Article", "created": "2021-05-22", "title": "Simultaneous removal of hydrocarbons and sulfate from groundwater using a \u201cbioelectric well\u201d", "description": "Abstract   Petroleum hydrocarbons (PHs) are often found in groundwater due to human activities like accidental spills, causing health and environmental risks, and requiring remediation. Microbial Electrochemical Technologies (METs) have emerged as a promising alternative to conventional bioremediation techniques for the treatment of PH-contaminated groundwater. However, the field-application of these promising sustainable as well as cost-effective technologies is still scarce. One major reason is the lack of scalable reactor configurations. Herein, an upgraded version of the \u201cbioelectric well\u201d, a novel tubular bioelectrochemical reactor that can be installed directly within a groundwater well, was tested for the simultaneous removal of oxidableoxidizable (i.e., toluene and other PH) and reducible (i.e., sulfate) compounds from a real contaminated groundwater.  After a proof-of-concept study in batch mode, the system was operated in continuous-flow mode for 48 days with the anode polarized at 0.2\u00a0V vs. SHE and a hydraulic retention time of 11\u00a0h. In these conditions, a steady-state removal rate of toluene as high as 31\u00a0\u00b1\u00a02\u00a0mg L\u22121 d\u22121 was achieved, which was more than double the value observed with the open circuit potential (OCP) control and one of the highest reported in literature. The electrode polarization went along with a higher abundance of key-functional genes involved in toluene degradation. This was not only showing its clear functional connection to the microbial metabolism, but further allowed to identify the involved electrogenic biodegradation pathway. In addition, the system simultaneously removed sulfate (30\u00a0\u00b1\u00a01\u00a0mg L\u22121 d\u22121), with bacteria likely using the H2 generated at the cathode as electron donor. Nevertheless, the apparent sulfate removal rate in the polarized and in the OCP runs was similar. The analysis of the microbial communities evidenced a high abundance of the genus Chlorobium in the effluent of the polarized run. These microorganisms were probably responsible for the continuous oxidative regeneration of sulfate from the sulfide produced at the cathode by sulfate-reducing bacteria. This phenomenon probably hindered the overall removal of sulfate by the bioelectrochemical system.", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Microbial electrochemical technologies", "Groundwater remediation", "bioremediation; groundwater remediation; microbial electrochemical technologies; petroleum hydrocarbons; toluene", "Petroleum hydrocarbons", "Bioremediation", "6. Clean water", "Toluene"]}, "links": [{"href": "https://doi.org/10.1016/j.electacta.2021.138636"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Electrochimica%20Acta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.electacta.2021.138636", "name": "item", "description": "10.1016/j.electacta.2021.138636", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.electacta.2021.138636"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-08-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2005.01.007", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:16:46Z", "type": "Journal Article", "created": "2005-03-15", "title": "Organochlorine Compounds In Soils And Sediments Of The Mountain Andean Lakes", "description": "Semi-volatile organochlorine compounds (OC) were analyzed in remote Andean soils and lake sediments. The sampling sites covered a wide latitudinal gradient from 18 degrees S to 46 degrees S along Chile and an altitudinal gradient (10-4500 m). The concentrations were in the order of background levels, involving absence of major pollution sources in the high mountain areas. Significant correlations were found between log-transformed concentrations of hexachlorobenzene, alpha- and gamma-hexachlorocyclohexane in soils and total organic content (TOC). In addition, TOC-normalized concentrations of the most volatile OC showed a significant linear dependence with air temperature. This good agreement points to temperature as a significant factor for the retention of long range transported OC in remote ecosystems such as the Andean mountains, although other variables should not be totally excluded. The highest concentrations of OCs were achieved in the sites located at highest altitude and lowest temperature of the dataset.", "keywords": ["Geologic Sediments", "Altitude", "lake sediments", "Temperature", "organochlorine compounds; Andean range; long range transport; soil; lake sediments", "Fresh Water", "Organochlorine compounds", "15. Life on land", "01 natural sciences", "Lake sediments", "soil", "organochlorine compound", "Andean range", "Long range transport", "Soil", "13. Climate action", "Hydrocarbons", " Chlorinated", "Soil Pollutants", "Chile", "long range transport", "Environmental Monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2005.01.007"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2005.01.007", "name": "item", "description": "10.1016/j.envpol.2005.01.007", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2005.01.007"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-07-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2010.02.003", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:16:47Z", "type": "Journal Article", "created": "2010-03-15", "title": "Effects Of Biochar And Greenwaste Compost Amendments On Mobility, Bioavailability And Toxicity Of Inorganic And Organic Contaminants In A Multi-Element Polluted Soil", "description": "Applying amendments to multi-element contaminated soils can have contradictory effects on the mobility, bioavailability and toxicity of specific elements, depending on the amendment. Trace elements and PAHs were monitored in a contaminated soil amended with biochar and greenwaste compost over 60 days field exposure, after which phytotoxicity was assessed by a simple bio-indicator test. Copper and As concentrations in soil pore water increased more than 30 fold after adding both amendments, associated with significant increases in dissolved organic carbon and pH, whereas Zn and Cd significantly decreased. Biochar was most effective, resulting in a 10 fold decrease of Cd in pore water and a resultant reduction in phytotoxicity. Concentrations of PAHs were also reduced by biochar, with greater than 50% decreases of the heavier, more toxicologically relevant PAHs. The results highlight the potential of biochar for contaminated land remediation.", "keywords": ["04 agricultural and veterinary sciences", "01 natural sciences", "United Kingdom", "6. Clean water", "Trace Elements", "Soil", "13. Climate action", "Metals", " Heavy", "Lolium", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Adsorption", "Polycyclic Aromatic Hydrocarbons", "Environmental Restoration and Remediation", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2010.02.003"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2010.02.003", "name": "item", "description": "10.1016/j.envpol.2010.02.003", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2010.02.003"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-06-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2012.12.014", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:16:47Z", "type": "Journal Article", "created": "2013-01-19", "title": "Reduced Bioaccumulation Of Pahs By Lactuca Satuva L. Grown In Contaminated Soil Amended With Sewage Sludge And Sewage Sludge Derived Biochar", "description": "The influence of sewage sludge (SS) and sewage sludge biochar (SSBC) upon biomass yield and the bioaccumulation of PAHs into lettuce plants grown in contaminated soil (\u221116PAH 20.2 \u00b1 0.9 mg kg(-1)) is presented. All SSBC amendments (2, 5 and 10%) and the 2% SS amendment significantly (P < 0.01) increased lettuce biomass. Both SS and SSBC amendments significantly reduced (P < 0.01) the bioaccumulation of PAHs at all application levels; with reduction in \u221116PAH concentration ranging between 41.8 and 60.3% in SS amended treatments and between 58.0 and 63.2% in SSBC amended treatments, with respect to the control. Benefits in terms of biomass production and PAHs bioaccumulation reduction were greatest where SSBC was used as a soil amendment. At high application rates (10%) SSBC reduced bioaccumulation of PAHs by between 56% and 67%, while SS reduced bioaccumulation of PAHs by less than 44%.", "keywords": ["Soil", "Sewage", "Charcoal", "Soil Pollutants", "Agriculture", "Polycyclic Aromatic Hydrocarbons", "Waste Disposal", " Fluid", "01 natural sciences", "Environmental Restoration and Remediation", "6. Clean water", "Lactuca", "0105 earth and related environmental sciences", "12. Responsible consumption"], "contacts": [{"organization": "Sardar Khan, Chao Cai, Ning Wang, Alessia Freddo, Alessia Freddo, Brian J. Reid,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2012.12.014"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2012.12.014", "name": "item", "description": "10.1016/j.envpol.2012.12.014", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2012.12.014"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-04-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2014.09.014", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:16:47Z", "type": "Journal Article", "created": "2014-10-09", "title": "High Concentrations Of Polycyclic Aromatic Hydrocarbons (Naphthalene, Phenanthrene And Pyrene) Failed To Explain Biochar'S Capacity To Reduce Soil Nitrous Oxide Emissions", "description": "The presence of polycyclic aromatic hydrocarbons (PAHs) has been postulated as a mechanism by which biochar might mitigate N(2)O emissions. We studied whether and to what extent N(2)O emissions were influenced by the three most abundant PAHs in biochar: naphthalene, phenanthrene and pyrene. We hypothesised that biochars contaminated with PAHs would show a larger N(2)O mitigation capacity and that increasing PAH concentrations in biochar would lead to higher mitigation potentials. Our results demonstrate that the high-temperature biochar (550 \u00b0C) had a higher capacity to mitigate soil N(2)O emissions than the low-temperature biochar (350 \u00b0C). At low PAH concentrations, PAHs do not significantly contribute to the reductions in soil N(2)O emissions; while biochar stimulated soil N(2)O emissions when it was spiked with high concentrations of PAHs. This study suggests that the impact of biochar on soil N(2)O emissions is due to other compositional and/or structural properties of biochar rather than to PAH concentration.", "keywords": ["2. Zero hunger", "Hot Temperature", "Pyrenes", "Nitrous Oxide", "04 agricultural and veterinary sciences", "Naphthalenes", "Phenanthrenes", "15. Life on land", "7. Clean energy", "01 natural sciences", "6. Clean water", "12. Responsible consumption", "Soil", "Models", " Chemical", "13. Climate action", "Charcoal", "11. Sustainability", "0401 agriculture", " forestry", " and fisheries", "Polycyclic Aromatic Hydrocarbons", "Environmental Restoration and Remediation", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2014.09.014"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2014.09.014", "name": "item", "description": "10.1016/j.envpol.2014.09.014", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2014.09.014"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-01-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2022.120472", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:16:48Z", "type": "Journal Article", "created": "2022-10-19", "title": "Comparative toxicological assessment of three soils polluted with different levels of hydrocarbons and heavy metals using in vitro and in vivo approaches", "description": "The biological effects induced by the pollutants present in soils, together with the chemical and physical characterizations, are good indicators to provide a general overview of their quality. However, the existence of studies where the toxicity associated to soils contaminated with mixtures of pollutants applying both in vitro and in vivo models are scarce. In this work, three soils (namely, Soil 001, Soil 002 and Soil 013) polluted with different concentrations of hydrocarbons and heavy metals were evaluated using different organisms representative of human (HepG2 human cell line) and environmental exposure (the yeast Saccharomyces cerevisiae, the Gram-negative bacterium Pseudomonas putida and, for the in vivo evaluation, the annelid Enchytraeus crypticus). In vitro assays showed that the soluble fraction of the Soil 001, which presented the highest levels of heavy metals, represented a great impact in the viability of the HepG2 cells and S. cerevisiae, while organic extracts from Soils 002 and 013 caused a slight decrease in the viability of HepG2 cells. In addition, in vivo experiments showed that Soils 001 and 013 affected the survival and the reproduction of E. crypticus. Altogether, these results provide a general overview of the potential hazards associated to three specific contaminated sites in a variety of organisms, showing how different concentrations of similar pollutants affect them, and highlights the relevance of testing both organic and soluble extracts when in vitro safety assays of soils are performed.", "keywords": ["Pseudomonas putida", "Enchytraeus crypticus", "Qu\u00edmica anal\u00edtica", "Saccharomyces cerevisiae", "Analytic", "01 natural sciences", "Hydrocarbons", "6. Clean water", "HepG2 cellsSaccharomyces cerevisiaePseudomonas putidaEnchytraeus crypticusSoil contamination", "Chemistry", "Soil", "Soil contamination", "13. Climate action", "Metals", " Heavy", "Humans", "Soil Pollutants", "Chemistry", " Analytic", "HepG2 cells Saccharomyces cerevisiae Pseudomonas putida Enchytraeus crypticus Soil contamination", "HepG2 cells", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2022.120472"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2022.120472", "name": "item", "description": "10.1016/j.envpol.2022.120472", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2022.120472"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-01T00:00:00Z"}}, {"id": "10.1016/j.envres.2024.118880", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:16:48Z", "type": "Journal Article", "created": "2024-04-04", "title": "Unveiling the capacity of bioaugmentation application, in comparison with biochar and rhamnolipid for TPHs degradation in aged hydrocarbons polluted soil", "description": "Persistent, aged hydrocarbons in soil hinder remediation, posing a significant environmental threat. While bioremediation offers an environmentally friendly and cost-effective approach, its efficacy for complex contaminants relies on enhancing pollutant bioavailability. This study explores the potential of immobilized bacterial consortia combined with biochar and rhamnolipids to accelerate bioremediation of aged total petroleum hydrocarbon (TPH)-contaminated soil. Previous research indicates that biochar and biosurfactants can increase bioremediation rates, while mixed consortia offer sequential degradation and higher hydrocarbon mineralization. The present investigation aimed to assess whether combining these strategies could further enhance degradation in aged, complex soil matrices. The bioaugmentation (BA) with bacterial consortium increased the TPHs degradation in aged soil (over 20% compared to natural attenuation - NA). However, co-application of BA with biochar and rhamnolipid higher did not show a statistically prominent synergistic effect. While biochar application facilitated the maintenance of hydrocarbon degrading bacterial consortium in soil, the present study did not identify a direct influence in TPHs degradation. The biochar application in contaminated soil contributed to TPHs adsorption. Rhamnolipid alone slightly increased the TPHs biodegradation with NA, while the combined bioaugmentation treatment with rhamnolipid and biochar increased the degradation between 27.5 and 29.8%. These findings encourage further exploration of combining bioaugmentation with amendment, like biochar and rhamnolipid, for remediating diverse environmental matrices contaminated with complex and aged hydrocarbons.", "keywords": ["Qu\u00edmica agr\u00edcola", "Bioqu\u00edmica", "Biolog\u00eda molecular", "Rhamnolipids", "Molecular biology", "Chemistry", " Inorganic", "Biochemistry", "Qu\u00edmica inorg\u00e1nica", "Hydrocarbons", "Inorganic", "Chemistry", "Biochar", "Soil", "Bioaugmentation", "Agricultural chemistry", "Biodegradation", " Environmental", "Petroleum", "Recalcitrant hydrocarbons", "Charcoal", "Biodegradation", "Soil Pollutants", "TPHs polluted soils", "Glycolipids", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.envres.2024.118880"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envres.2024.118880", "name": "item", "description": "10.1016/j.envres.2024.118880", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envres.2024.118880"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-01T00:00:00Z"}}, {"id": "10.1016/j.ese.2020.100013", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:16:49Z", "type": "Journal Article", "created": "2020-01-13", "title": "Microbial electrochemistry for bioremediation", "description": "Lack of suitable electron donors or acceptors is in many cases the key reason for pollutants to persist in the environment. Externally supplementation of electron donors or acceptors is often difficult to control and/or involves chemical additions with limited lifespan, residue formation or other adverse side effects. Microbial electrochemistry has evolved very fast in the past years - this field relates to the study of electrochemical interactions between microorganisms and solid-state electron donors or acceptors. Current can be supplied in such so-called bioelectrochemical systems (BESs) at low voltage to provide or extract electrons in a very precise manner. A plethora of metabolisms can be linked to electrical current now, from metals reductions to denitrification and dechlorination. In this perspective, we provide an overview of the emerging applications of BES and derived technologies towards the bioremediation field and outline how this approach can be game changing.", "keywords": ["0301 basic medicine", "AUTOTROPHIC DENITRIFICATION", "elecetrobioremediation", "Bioremediaci\u00f3", "FUEL-CELLS", "Environmental technology. Sanitary engineering", "Microbial biotechnology", "01 natural sciences", "POLYCYCLIC AROMATIC-HYDROCARBONS", "03 medical and health sciences", "WASTE-WATER", "DECHLORINATION", "TD Environmental technology. Sanitary engineering", "Electrochemistry", "POLLUTANTS", "GE1-350", "TD1-1066", "0105 earth and related environmental sciences", "NITRATE-CONTAMINATED GROUNDWATER", "ENVIRONMENTAL REMEDIATION", "Q Science (General)", "QR Microbiology", "NITROGEN REMOVAL", "6. Clean water", "Environmental sciences", "Electroqu\u00edmica", "ORGANIC", "BIOELECTROCHEMICAL SYSTEMS", "13. Climate action", "Earth and Environmental Sciences", "Perspective", "Biotecnologia microbiana", "Bioremediation"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/540323/1/1-s2.0-S2666498420300053-main.pdf"}, {"href": "https://doi.org/10.1016/j.ese.2020.100013"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Ecotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ese.2020.100013", "name": "item", "description": "10.1016/j.ese.2020.100013", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ese.2020.100013"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-01T00:00:00Z"}}, {"id": "10.1016/j.jece.2020.104657", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:17:13Z", "type": "Journal Article", "created": "2020-10-24", "title": "Reductive/oxidative sequential bioelectrochemical process for Perchloroethylene (PCE) removal: effect of the applied reductive potential and microbial community characterization", "description": "Abstract   In this paper, a bioelectrochemical process has been developed by the combination of two membrane-less reactors equipped with an internal graphite granules counterelectrode for the perchloroethylene (PCE) removal through a reductive/oxidative sequence. In the reductive reactor, the cathodic chamber supplied the reducing power to PCE dechlorinating biomass while a rutile electrode promoted the aerobic dechlorination of the less chlorinated PCE byproducts by oxygen in situ evolution. Two potentiostatic conditions, -350 and -550 mV vs SHE, were tested on the reductive reactor, which showed the capability to completely reduce the PCE into vinyl chloride (VC) and ethylene (Eth). These compounds were completely removed by the oxidative reactor with an average VC and Eth removal efficiency of 94 \u00b1 1% and 98 \u00b1 1%. The -350 mV vs SHE condition resulted in the higher coulombic efficiency for the reductive dechlorination which reached 22 \u00b1 7 % while by increasing the reductive potential to -550 mV the coulombic efficiency drop down to 6 \u00b1 1 % in favor of the methanogenesis reaction. Dehalococcoides mccartyi was found at high abundance in the reducing reactor while a heterogeneous bacterial consortium was observed in the oxidative reactor. Microbiome characterization of the reductive and oxidative reactors showed the concomitant presence of different redox niches in each compartment suggesting that the exchange of ionic species between the electrode and the counterelectrode allowed the co-existence of both reducing and oxidative reactions.", "keywords": ["0301 basic medicine", "0303 health sciences", "EC", "reductive dechlorination; oxidative dechlorination; bioremediation; bioelectrochemical systems; chlorinated aliphatic hydrocarbons; groundwater remediation", "Reductive dechlorination", "Process Chemistry and Technology", "H2020", "Pollution", "Horizon 2020 Framework Programme", "6. Clean water", "Research and Innovation action", "Bioelectrochemical systems", "03 medical and health sciences", "bioremediation", "Chemical Engineering (miscellaneous)", "European Commission", "Waste Management and Disposal"]}, "links": [{"href": "https://doi.org/10.1016/j.jece.2020.104657"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Chemical%20Engineering", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jece.2020.104657", "name": "item", "description": "10.1016/j.jece.2020.104657", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jece.2020.104657"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-01T00:00:00Z"}}, {"id": "10.1016/j.jconhyd.2018.03.004", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:17:13Z", "type": "Journal Article", "created": "2018-03-18", "title": "Comparison of theory and experiment for NAPL dissolution in porous media", "description": "Contamination of groundwater resources by an immiscible organic phase commonly called NAPL (Non Aqueous Phase Liquid) represents a major scientific challenge considering the residence time of such a pollutant. This contamination leads to the formation of NAPL blobs trapped in the soil and impact of this residual saturation cannot be ignored for correct predictions of the contaminant fate. In this paper, we present results of micromodel experiments on the dissolution of pure hydrocarbon phase (toluene). They were conducted for two values of the P\u00e9clet number. These experiments provide data for comparison and validation of a two-phase non-equilibrium theoretical model developed by Quintard and Whitaker (1994) using the volume averaging method. The model was directly upscaled from the averaged pore-scale mass balance equations. The effective properties of the macroscopic model were calculated over periodic unit cells designed from images of the experimental flow cell. Comparison of experimental and numerical results shows that the transport model predicts correctly - with no fitting parameters - the main mechanisms of NAPL mass transfer. The study highlights the crucial need of having a fair recovery of pore-scale characteristic lengths to predict the mass transfer coefficient with accuracy.", "keywords": ["Volume averaging method", "[SPI.FLUID]Engineering Sciences [physics]/Reactive fluid environment", "0208 environmental biotechnology", "Porous media", "0207 environmental engineering", "02 engineering and technology", "Models", " Theoretical", "Porous media flow", "Hydrocarbons", "6. Clean water", "[SPI.MECA.MEFL]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Fluids mechanics [physics.class-ph]", "Soil", "Solubility", "Upscaling transport", "13. Climate action", "Volume Averaging", "Upscaling", "NAPL dissolution", "[SDU.STU.HY]Sciences of the Universe [physics]/Earth Sciences/Hydrology", "Hydrology", "Groundwater", "Porosity", "Water Pollutants", " Chemical", "Toluene"]}, "links": [{"href": "https://doi.org/10.1016/j.jconhyd.2018.03.004"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Contaminant%20Hydrology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jconhyd.2018.03.004", "name": "item", "description": "10.1016/j.jconhyd.2018.03.004", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jconhyd.2018.03.004"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-04-01T00:00:00Z"}}, {"id": "10.1016/j.jece.2022.107799", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:17:13Z", "type": "Journal Article", "created": "2022-04-27", "title": "\"Syntrophy drives the microbial electrochemical oxidation of toluene in a continuous-flow \"\"bioelectric well\"\"\"", "description": "Microbial electrochemical technologies (MET) are promising for the remediation of groundwater pollutants such as petroleum hydrocarbons (PH). Indeed, MET can provide virtually inexhaustible electron donors or acceptors directly in the subsurface environment. However, the degradation mechanisms linking contaminants removal to electric current flow are still largely unknown, hindering the development of robust design criteria. Here, we analysed the degradation of toluene, a model PH, in a bioelectrochemical reactor known as 'bioelectric well'operated in continuous-flow mode at various influent toluene concentrations. With increasing concentration of toluene, the removal rate increased while the current tended to a plateau, hence the columbic efficiency decreased. Operation at open circuit confirmed that the bioelectrochemical degradation of toluene proceeded via a syntrophic pathway involving cooperation between different microbial populations. First of all, hydrocarbon degraders quickly converted toluene into metabolic intermediates probably by breaking the aromatic ring upon fumarate addition. Subsequently, fermentative bacteria converted these intermediates into volatile fatty acids (VFA) and likely also H2, which were then used as substrates by electroactive microorganisms forming the anodic biofilm. As toluene degradation is faster than subsequent conversion steps, the increase in intermediate concentration could not result in a current increase. This work provides valuable insights on the syntrophic degradation of BTEX, which are essential for the application of microbial electrochemical system to groundwater remediation of petroleum hydrocarbons.", "keywords": ["Microbial electrochemical technologies", "13. Climate action", "Groundwater remediation", "bioremediation; groundwater remediation; microbial electrochemical technologies; petroleum hydrocarbons; toluene", "Petroleum hydrocarbons", "01 natural sciences", "Bioremediation", "6. Clean water", "Toluene", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/475025/1/Syntrophy%20drives%20the%20microbial%20electrochemical%20oxidation%20of%20toluene%20in%20a%20continuous-flow%20%e2%80%9cbioelectric%20well%e2%80%9d.pdf"}, {"href": "https://iris.uniroma1.it/bitstream/11573/1661830/2/Tucci_Syntrophy-drives-microbial_2022.pdf"}, {"href": "https://doi.org/10.1016/j.jece.2022.107799"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Chemical%20Engineering", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jece.2022.107799", "name": "item", "description": "10.1016/j.jece.2022.107799", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jece.2022.107799"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-06-01T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2007.07.014", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:17:15Z", "type": "Journal Article", "created": "2007-07-11", "title": "Accumulation Of Polycyclic Aromatic Hydrocarbons And Heavy Metals In Lettuce Grown In The Soils Contaminated With Long-Term Wastewater Irrigation", "description": "Accumulation of polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs) by crop plants from contaminated soils may pose health risks. A greenhouse pot experiment using lettuce (Lactuca satuva L.) as a representative vegetable was conducted to assess the concentrations of PAHs and HMs in vegetables grown in wastewater-contaminated soils. The concentrations of total PAHs were ranged from 1.5 to 3.4 mg kg(-1) in the contaminated soils, while 1.2 mg kg(-1) in the reference soil. Linear regression analyses showed that the relationships between soil and shoot PAH concentrations were stronger for LMW-PAHs (R(2) between 0.51 and 0.92) than for HMW-PAHs (R(2) 0.02 and 0.60), suggesting that translocation for LMW-PAHs is faster than HMW-PAHs. Furthermore, the data imply that root uptake was the main pathway for HMW-PAHs accumulation. The plant shoots were also highly contaminated with HMs, particularly Cd (0.4-0.9 mg kg(-1)), Cr (3.4-4.1 mg kg(-1)), Ni (11.7-15.1 mg kg(-1)) and Pb (2.3-5.3 mg kg(-1)), and exceed the guidance limits set by State Environmental Protection Administration (SEPA), China and the World Health Organization (WHO). This study highlights the potential health risks associated with cultivation and consumption of leafy vegetables on wastewater-contaminated soils.", "keywords": ["2. Zero hunger", "Agriculture", "Food Contamination", "15. Life on land", "01 natural sciences", "6. Clean water", "3. Good health", "Waste Management", "13. Climate action", "Metals", " Heavy", "11. Sustainability", "Soil Pollutants", "Polycyclic Aromatic Hydrocarbons", "Water Pollutants", " Chemical", "Lactuca", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.jhazmat.2007.07.014"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2007.07.014", "name": "item", "description": "10.1016/j.jhazmat.2007.07.014", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2007.07.014"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-04-01T00:00:00Z"}}, {"id": "10.1016/j.jwpe.2022.103101", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:17:18Z", "type": "Journal Article", "created": "2022-09-08", "title": "Evaluation of a bioelectrochemical reductive/oxidative sequential process for chlorinated aliphatic hydrocarbons (CAHs) removal from a real contaminated groundwater", "description": "In the present study, the sequential reductive/oxidative bioelectrochemical process has been tested with real groundwater from a contaminated site in Northern Italy for chlorinated aliphatic hydrocarbons (CAHs) removal. The sequential system was developed by connecting in series two membrane-less microbial electrolysis cells (MECs) equipped with an internal graphite counter electrode. The first MEC aimed at the CAHs reductive dechlorination (RD) and was constituted of a granular graphite working electrode. In the second MEC, a mixed metal oxide working electrode stimulated the oxidative dechlorination of the low chlorinated RD's by-products through oxygen production. The sequential process allowed complete mineralization of the CAHs contained in the real groundwater. A complete reduction of the perchloroethylene into vinyl chloride (VC) was observed in the first MEC polarized at \u2212450 mV vs SHE, while the resulting VC was oxidized with a 92 \u00b1 2 % efficiency in the second MEC due to the HRT increment from 0.7 to 1.7 days. Biomarkers of the reductive (Dehalococcoides mccartyi 16S rRNA and reductive dehalogenase genes) and oxidative (etnE, etnC genes) dechlorination have been monitored in the two MECs along with the ecotoxicity tests. Overall, they provide information on the efficiency of the applied technology and allow to assess the potential adverse effects. According to the Tetrahymena pyriformis reproduction inhibition test and Panagrellus redivivus mortality tests, showed a significant ecotoxicity reduction with respect its initial inhibitory effect at the tested concentrations.", "keywords": ["Chlorinated aliphatic hydrocarbons", "Microbial electrolysis cells", "Reductive dechlorination", "biomarkers", " chlorinated aliphatic hydrocarbons", " ecotoxicity", " microbial electrolysis cells", " oxidative dechlorination", " reductive dechlorination", "Oxidative dechlorination", "Ecotoxicity", "Biomarkers"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/538331/1/1-s2.0-S2214714422005451-main.pdf"}, {"href": "https://iris.uniroma1.it/bitstream/11573/1655075/1/1-s2.0-S2214714422005451-main.pdf"}, {"href": "https://doi.org/10.1016/j.jwpe.2022.103101"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Water%20Process%20Engineering", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jwpe.2022.103101", "name": "item", "description": "10.1016/j.jwpe.2022.103101", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jwpe.2022.103101"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-10-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2019.03.176", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:17:28Z", "type": "Journal Article", "created": "2019-03-13", "title": "Effect of digestate application on microbial respiration and bacterial communities' diversity during bioremediation of weathered petroleum hydrocarbons contaminated soils", "description": "Digestate is an organic by-product of biogas production via anaerobic digestion processes and has a great potential as soil fertilizer due to concentrated nutrients. In this study, we examined digestate as a potential nutrient and microbial seeding for bioremediation of weathered (aged) petroleum hydrocarbon contaminated soils. We analysed 6 different treatments in microcosm using two industrial soils having different textures: a clay rich soil and a sandy soil. After 30\u202fdays of incubation, the highest total petroleum hydrocarbons (TPH) removal was observed in microcosms containing digestate together with bulking agent (17.8% and 12.7% higher than control in clay rich soil and sandy soil, respectively) or digestate together with immobilized bacteria (13.4% and 9% higher than control in clay rich soil and sandy soil, respectively). After digestate application microbial respiration was enhanced in sandy soil and inhibited in clay rich soil due to aggregates formation. After bulking agent addition to clay rich soil aggregates size was reduced and oxygen uptake was improved. Application of digestate to soil resulted in the development of distinct microbial groups in amended and non-amended soils. Genera containing species able to degrade TPH like Acinetobacter and Mycobacterium were abundant in digestate and in soil amended with digestate. Quantification of alkB genes, encoding alkane monoxygenase, revealed high concentration of these genes in digestate bacterial community. After application of digestate, the level of alkB genes significantly increased in soils and remained high until the end of the treatment. The study revealed great potential of digestate as a nutrient and bacteria source for soil bioremediation.", "keywords": ["[SDE] Environmental Sciences", "TPH removal", "550", "Soil remediation", "0211 other engineering and technologies", "alkB genes; Organic fertilizers; qPCR; Soil remediation; TPH removal; Biodegradation", " Environmental; Environmental Restoration and Remediation; Hydrocarbons; Petroleum; Petroleum Pollution; Soil; Soil Pollutants; Soil Microbiology", "02 engineering and technology", "01 natural sciences", "7. Clean energy", "630", "Organic fertilizers", "Environmental", "Soil", "alkB genes", "Soil Pollutants", "Petroleum Pollution", "Environmental Restoration and Remediation", "Soil Microbiology", "0105 earth and related environmental sciences", "2. Zero hunger", "[SDE.IE]Environmental Sciences/Environmental Engineering", "15. Life on land", "Hydrocarbons", "6. Clean water", "qPCR", "Biodegradation", " Environmental", "Petroleum", "[SDE]Environmental Sciences", "Biodegradation", "[SDE.IE] Environmental Sciences/Environmental Engineering"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2019.03.176"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2019.03.176", "name": "item", "description": "10.1016/j.scitotenv.2019.03.176", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2019.03.176"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2019.135503", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:17:28Z", "type": "Journal Article", "created": "2019-11-17", "title": "Isomer-selective biodegradation of high-molecular-weight azaarenes in PAH-contaminated environmental samples", "description": "Polycyclic aromatic nitrogen heterocycles, or azaarenes, normally co-occur with polycyclic aromatic hydrocarbons (PAHs) in contaminated soils. We recently reported that nontarget analysis using high resolution mass spectrometry of samples from four PAH-contaminated sites revealed a previously unrecognized diversity and abundance of azaarene isomers and their methylated derivatives. Here we evaluated their biodegradability by natural microbial communities from each site in aerobic microcosm incubations under biostimulated conditions. The removal of total quantifiable azaarenes ranged from 15 to 85%, and was related to the initial degree of weathering for each sample. While three-ring azaarenes were readily biodegradable, the five-ring congeners were the most recalcitrant. Microbial-mediated removal of four-ring congeners varied for different isomers, which might be attributed to the position of the nitrogen atom that also influences the physicochemical properties of azaarenes and possibly the susceptibility to transformation by relevant microbial enzymes. The presence of methyl groups also influenced azaarene biodegradability, which decreased with increasing degree of methylation. Several oxidation products of azaarenes were detected, including ketones and dioxygenated derivatives of three- and four-ring compounds. Our results indicate the susceptibility of some azaarenes to bioremediation, while suggesting the potential implications for risk from the persistence of less-biodegradable isomers and the formation of oxidized-azaarene derivatives.", "keywords": ["0301 basic medicine", "03 medical and health sciences", "Biodegradation", " Environmental", "13. Climate action", "Soil Pollutants", "Polycyclic Aromatic Hydrocarbons", "01 natural sciences", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2019.135503"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2019.135503", "name": "item", "description": "10.1016/j.scitotenv.2019.135503", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2019.135503"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2022.157325", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:17:29Z", "type": "Journal Article", "created": "2022-07-14", "title": "Passive electrobioremediation approaches for enhancing hydrocarbons biodegradation in contaminated soils,", "description": "Electrobioremediation technologies hold considerable potential for the treatment of soils contaminated by petroleum hydrocarbons (PH), since they allow stimulating biodegradation processes with no need for subsurface chemicals injection and with little to no energy consumption. Here, a microbial electrochemical snorkel (MES) was applied for the treatment of a soil contaminated by hydrocarbons. The MES consists of direct coupling of a microbial anode with a cathode, being a single conductive, non-polarized material positioned suitably to create an electrochemical connection between the anoxic zone (the contaminated soil) and the oxic zone (the overlying oxygenated water). Soil was also supplemented with electrically conductive particles of biochar as a strategy to construct a conductive network with microbes in the soil matrix, thus extending the radius of influence of the snorkel. The results of a comprehensive suite of chemical, microbiological and ecotoxicological analyses evidenced that biochar addition, rather than the presence of a snorkel, was the determining factor in accelerating PH removal from contaminated soils, possibly accelerating syntrophic and/or cooperative metabolisms involved in the degradation of PH. The enhancement of biodegradation was mirrored by an increased abundance of anaerobic and aerobic microorganisms known to be involved in the degradation of PH and related functional genes. Plant ecotoxicity assays confirmed a reduction of soils toxicity in treatments receiving electrically conductive biochar.", "keywords": ["02 engineering and technology", "01 natural sciences", "Hydrocarbons", "6. Clean water", "Biochar", "Soil", "Biodegradation", " Environmental", "Petroleum", "Soil Pollutants", "Contaminated soil", "Microbial electrochemical snorkel", "biochar; bioelectrochemical system; bioremediation; contaminated soil; microbial electrochemical snorkel; petroleum hydrocarbons", "0210 nano-technology", "Petroleum hydrocarbons", "Bioremediation", "Soil Microbiology", "Bioelectrochemical system", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2022.157325"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2022.157325", "name": "item", "description": "10.1016/j.scitotenv.2022.157325", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2022.157325"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-11-01T00:00:00Z"}}, {"id": "10.1021/acs.est.2c03149", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:03Z", "type": "Journal Article", "created": "2022-09-14", "title": "Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media", "description": "Recent research has demonstrated that chemotactic bacteria can disperse inside microsized pores while traveling toward favorable conditions. Microbe-microbe cotransport might enable nonmotile bacteria to be carried with motile partners to enhance their dispersion and reduce their deposition in porous systems. The aim of this study was to demonstrate the enhancement in the dispersion of nonmotile bacteria (Mycobacterium gilvum VM552, a polycyclic aromatic hydrocarbon-degrader, and Sphingobium sp. D4, a hexachlorocyclohexane-degrader, through micrometer-sized pores near the exclusion-cell-size limit, in the presence of motile Pseudomonas putida G7 cells. For this purpose, we used bioreactors equipped with two chambers that were separated with membrane filters with 3, 5, and 12 \u03bcm pore sizes and capillary polydimethylsiloxane (PDMS) microarrays (20 \u03bcm \u00d7 35 \u03bcm \u00d7 2.2 mm). The cotransport of nonmotile bacteria occurred exclusively in the presence of a chemoattractant concentration gradient, and therefore, a directed flow of motile cells. This cotransport was more intense in the presence of larger pores (12 \u03bcm) and strong chemoeffectors (\u03b3-aminobutyric acid). The mechanism that governed cotransport at the cell scale involved mechanical pushing and hydrodynamic interactions. Chemotaxis-mediated cotransport of bacterial degraders and its implications in pore accessibility opens new avenues for the enhancement of bacterial dispersion in porous media and the biodegradation of heterogeneously contaminated scenarios.", "keywords": ["Chemotactic Factors", "Pseudomonas putida", "Chemotaxis", "Bioaccessibility", "01 natural sciences", "Microbe-microbe cotransport", "Dimethylpolysiloxanes", "Polycyclic Aromatic Hydrocarbons", "Micrometer-sized pores", "Porosity", "Hexachlorocyclohexane", "gamma-Aminobutyric Acid", "Hitchhiking", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.2c03149"}, {"href": "https://doi.org/10.1021/acs.est.2c03149"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.est.2c03149", "name": "item", "description": "10.1021/acs.est.2c03149", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.est.2c03149"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-09-14T00:00:00Z"}}, {"id": "10.1021/acs.est.7b01172", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:03Z", "type": "Journal Article", "created": "2017-05-16", "title": "Nontarget Analysis Reveals a Bacterial Metabolite of Pyrene Implicated in the Genotoxicity of Contaminated Soil after Bioremediation", "description": "Bioremediation is an accepted technology for cleanup of soil contaminated with polycyclic aromatic hydrocarbons (PAHs), but it can increase the genotoxicity of the soil despite removal of the regulated PAHs. Although polar biotransformation products have been implicated as causative genotoxic agents, no specific product has been identified. We pursued a nontarget analytical approach combining effect-directed analysis (EDA) and metabolite profiling to compare extracts of PAH-contaminated soil from a former manufactured-gas plant site before and after treatment in a laboratory-scale aerobic bioreactor. A compound with the composition C15H8O2 and four methylated homologues were shown to accumulate as a result of bioreactor treatment, and the C15H8O2 compound purified from soil extracts was determined to be genotoxic. Its structure was established by nuclear magnetic resonance and mass spectroscopy as a heretofore unidentified \u03b1,\u03b2-unsaturated lactone derived from dioxygenation of pyrene at an apical ring, 2H-naphtho[2,1,8-def]chromen-2-one (NCO), which was confirmed by synthesis. The concentration of NCO in the bioreactor was 11 \u03bcg g-1 dry soil, corresponding to 13% of the pyrene removed. It also accumulated in aerobically incubated soil from two additional PAH-contaminated sites and was formed from pyrene by two pyrene-degrading bacterial cultures known to be geographically widespread, underscoring its potential environmental significance.", "keywords": ["2. Zero hunger", "Soil", "Biodegradation", " Environmental", "Pyrenes", "13. Climate action", "Soil Pollutants", "Polycyclic Aromatic Hydrocarbons", "01 natural sciences", "Soil Microbiology", "6. Clean water", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.7b01172"}, {"href": "https://doi.org/10.1021/acs.est.7b01172"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.est.7b01172", "name": "item", "description": "10.1021/acs.est.7b01172", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.est.7b01172"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-05-30T00:00:00Z"}}, {"id": "10.1021/acs.est.7b03319", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:04Z", "type": "Journal Article", "created": "2017-11-21", "title": "Diversity and Abundance of High-Molecular-Weight Azaarenes in PAH-Contaminated Environmental Samples", "description": "Azaarenes are N-heterocyclic polyaromatic pollutants that co-occur with polycyclic aromatic hydrocarbons (PAHs) in contaminated soils. Despite the known toxicity of some high-molecular-weight azaarenes, their diversity, abundance, and fate in contaminated soils remain to be elucidated. We applied high-resolution mass spectrometry and mass-defect filtering to four PAH-contaminated samples from geographically distant sites and detected 232 azaarene congeners distributed in eight homologous series, including alkylated derivatives and two hitherto unknown series. Four- and five-ring azaarenes were detected among these series, and the most abundant nonalkylated congeners groups (C13H9N, C15H9N, C17H11N, C19H11N, and C21H13N) were quantified. The profiles of congener groups varied among different sites. Three-ring azaarenes presented higher concentrations in unweathered sites, while four- and five-ring azaarenes predominated in weathered sites. Known toxic and carcinogenic azaarenes, such as benzo[c]acridine and dibenzo[a,h]acridine, were detected along with their multiple isomers. Our results highlight a previously unrecognized diversity and abundance of azaarenes in PAH-contaminated sites, with corresponding implications for environmental monitoring and risk assessment.", "keywords": ["13. Climate action", "11. Sustainability", "Polycyclic Aromatic Hydrocarbons", "Risk Assessment", "01 natural sciences", "Environmental Monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.7b03319"}, {"href": "https://doi.org/10.1021/acs.est.7b03319"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.est.7b03319", "name": "item", "description": "10.1021/acs.est.7b03319", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.est.7b03319"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-12-01T00:00:00Z"}}, {"id": "10.1021/es202970x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:05Z", "type": "Journal Article", "created": "2012-02-09", "title": "Effects Of Chemical, Biological, And Physical Aging As Well As Soil Addition On The Sorption Of Pyrene To Activated Carbon And Biochar", "description": "In this study, the suitability of biochar and activated carbon (AC) for contaminated soil remediation is investigated by determining the sorption of pyrene to both materials in the presence and absence of soil and before as well as after aging. Biochar and AC were aged either alone or mixed with soil via exposure to (a) nutrients and microorganisms (biological), (b) 60 and 110 \u00b0C (chemical), and (c) freeze-thaw cycles (physical). Before and after aging, the pH, elemental composition, cation exchange capacity (CEC), microporous SA, and sorption isotherms of pyrene were quantified. Aging at 110 \u00b0C altered the physicochemical properties of all materials to the greatest extent (for example, pH increased by up to three units and CEC by up to 50% for biochar). Logarithmic K(Fr) values ranged from 7.80 to 8.21 (ng kg(-1))(ng L(-1))(-nF) for AC and 5.22 to 6.21 (ng kg(-1))(ng L(-1))(-nF) for biochar after the various aging regimes. Grinding biochar to a smaller particle size did not significantly affect the sorption of d(10) pyrene, implying that sorption processes operate on the subparticle scale. Chemical aging decreased the sorption of pyrene to the greatest extent (up to 1.8 log unit for the biochar+soil). The sorption to AC was affected more by the presence of soil than the sorption to biochar was. Our results suggest that AC and biochar have a high sorption capacity for pyrene that is maintained both in the presence of soil and during harsh aging. Both materials could therefore be considered in contaminated land remediation.", "keywords": ["Pyrenes", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "Soil", "13. Climate action", "Charcoal", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Adsorption", "Polycyclic Aromatic Hydrocarbons", "Environmental Restoration and Remediation", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1021/es202970x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/es202970x", "name": "item", "description": "10.1021/es202970x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/es202970x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-11-28T00:00:00Z"}}, {"id": "10.3390/microorganisms13040848", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:21:40Z", "type": "Journal Article", "created": "2025-04-10", "title": "Assessing Microbial Activity and Rhizoremediation in Hydrocarbon and Heavy Metal-Impacted Soil", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Rhizodegradation enhances pollutant degradation through plant\u2013microbe interactions in the rhizosphere. Plant roots provide a colonisation surface and root exudates that promote microbial abundance and activity, facilitating organic pollutant breakdown via direct microbial degradation and co-metabolism. This study assessed the rhizodegradation of weathered petroleum hydrocarbons (PHCs) in heavy metal co-contaminated soil in a microcosm-scale pot trial. Treatments included Sinapis alba, Lolium perenne, a L. perenne + Trifolium repens mix, and Cichorium intybus, alongside a non-planted control. After 14 weeks, PHC concentrations were analysed via gas chromatography, and rhizosphere microbial communities were characterised through sequencing. Sinapis alba achieved the highest PHC degradation (68%), significantly exceeding the non-planted control (p &lt; 0.05, Kruskal\u2013Wallis test). Hydrocarbon-degrading bacteria, including KCM-B-112, C1-B045, Hydrogenophaga, unclassified Saccharimonadales sp., and Pedobacter, were enriched in the rhizosphere, with the uncultured clade mle1-27 potentially contributing indirectly. Metals analysis of plant tissues showed that mustard could accumulate copper more than lead and zinc, despite higher concentrations of zinc and lead in the soil. These results highlight the potential of S. alba for rhizoremediation in PHC\u2013heavy metal co-contaminated soils.</p></article>", "keywords": ["petroleum hydrocarbons", "bioremediation", "QH301-705.5", "microbial communities", "phytoremediation", "Biology (General)", "heavy metals", "rhizodegradation", "Article"], "contacts": [{"organization": "Robert Conlon, David N. Dowling, Kieran J. Germaine,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.3390/microorganisms13040848"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms13040848", "name": "item", "description": "10.3390/microorganisms13040848", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms13040848"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-04-08T00:00:00Z"}}, {"id": "10.1080/15226514.2024.2427925", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:18:55Z", "type": "Journal Article", "created": "2024-11-25", "title": "Application of herbaceous plant mixtures for remediation of TPH-contaminated soil", "description": "Soil pollution with petroleum products is an urgent public health and environmental problem. Therefore, innovative solutions for cleaning soils contaminated with petroleum products are needed. One such solution is rhizodegradation, which is recognized as a sustainable and effective method of in situ soil remediation. Much of the previous research was done with monocultures, therefore the effects of different combinations of plants on the removal of petroleum products remain ambiguous. These studies evaluated three different herbaceous plant mixtures for the removal of total petroleum hydrocarbons (TPHs) from contaminated soil. Promising results were obtained. Selected herbaceous plant species and their mixtures can be successfully grown in contaminated soil at a contamination level of 6,817\u2009mg/kg TPH DW according to the selected cultivation strategy. After applying a complex of biotechnology and agronomic solutions, the morphological and morphometric indicators revealed the good adaptability and tolerance of the selected herbaceous plants to growing in contaminated soil. After two years of pot testing application of different mixtures of herbaceous plants, the TPH (C6-C40) removal potential reached 85-90%.", "keywords": ["Soil", "Biodegradation", " Environmental", "Petroleum", "Soil Pollutants", "Plants", "Hydrocarbons"], "contacts": [{"organization": "Mantas, Rube\u017eius, \u017dygimantas, Kidikas, Alfreda, Kasiulien\u0117, Vilma, Keme\u0161yt\u0117, Vaclovas, Stukonis,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1080/15226514.2024.2427925"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Phytoremediation", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1080/15226514.2024.2427925", "name": "item", "description": "10.1080/15226514.2024.2427925", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1080/15226514.2024.2427925"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-11-25T00:00:00Z"}}, {"id": "10.1099/mgen.0.000363", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:07Z", "type": "Journal Article", "created": "2020-04-02", "title": "Analysis of the biodegradative and adaptive potential of the novel polychlorinated biphenyl degrader Rhodococcus sp. WAY2 revealed by its complete genome sequence", "description": "<p>The complete genome sequence of                                                Rhodococcus                                          sp. WAY2 (WAY2) consists of a circular chromosome, three linear replicons and a small circular plasmid. The linear replicons contain typical actinobacterial invertron-type telomeres with the central CGTXCGC motif. Comparative phylogenetic analysis of the 16S rRNA gene along with phylogenomic analysis based on the genome-to-genome blast distance phylogeny (GBDP) algorithm and digital DNA\uffe2\uff80\uff93DNA hybridization (dDDH) with other                                                Rhodococcus                                          type strains resulted in a clear differentiation of WAY2, which is likely a new species. The genome of WAY2 contains five distinct clusters of bph, etb and nah genes, putatively involved in the degradation of several aromatic compounds. These clusters are distributed throughout the linear plasmids. The high sequence homology of the ring-hydroxylating subunits of these systems with other known enzymes has allowed us to model the range of aromatic substrates they could degrade. Further functional characterization revealed that WAY2 was able to grow with biphenyl, naphthalene and xylene as sole carbon and energy sources, and could oxidize multiple aromatic compounds, including ethylbenzene, phenanthrene, dibenzofuran and toluene. In addition, WAY2 was able to co-metabolize 23 polychlorinated biphenyl congeners, consistent with the five different ring-hydroxylating systems encoded by its genome. WAY2 could also use n-alkanes of various chain-lengths as a sole carbon source, probably due to the presence of alkB and ladA gene copies, which are only found in its chromosome. These results show that WAY2 has a potential to be used for the biodegradation of multiple organic compounds.</p", "keywords": ["0301 basic medicine", "0303 health sciences", "PCB", "Whole Genome Sequencing", "AlkB Enzymes", "Rhodococcus; biodegradation; PAH; PCB; hydrocarbons; complete genome", "High-Throughput Nucleotide Sequencing", "PAH", "Naphthalenes", "Xylenes", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "biodegradation", "Polychlorinated Biphenyls", "Hydrocarbons", "Complete genome", "03 medical and health sciences", "Biodegradation", " Environmental", "RNA", " Ribosomal", " 16S", "Biodegradation", "Cluster Analysis", "Rhodococcus", "Phylogeny", "Research Article"]}, "links": [{"href": "https://doi.org/10.1099/mgen.0.000363"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Genomics", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1099/mgen.0.000363", "name": "item", "description": "10.1099/mgen.0.000363", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1099/mgen.0.000363"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-01T00:00:00Z"}}, {"id": "10.1111/1751-7915.13654", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:16Z", "type": "Journal Article", "created": "2020-08-31", "title": "An underappreciated DIET for anaerobic petroleum hydrocarbon\u2010degrading microbial communities", "description": "Direct interspecies electron transfer (DIET) via electrically conductive minerals can play a role in the anaerobic oxidation of petroleum hydrocarbons in contaminated sites and can be exploited for the development of new, more effective bioremediation approaches.", "keywords": ["0301 basic medicine", "H100", "0303 health sciences", "petroleum hydrocarbon", "anaerobic degradation", "Microbiota", "H800", "Hydrocarbons", "6. Clean water", "DIET", "03 medical and health sciences", "Biodegradation", " Environmental", "Petroleum", "Soil Pollutants", "Anaerobiosis", "Crystal Ball", "TP248.13-248.65", "Biotechnology"]}, "links": [{"href": "https://nrl.northumbria.ac.uk/id/eprint/44983/8/1751-7915.13654.pdf"}, {"href": "https://iris.cnr.it/bitstream/20.500.14243/394172/1/An%20underappreciated%20DIET%20for%20anaerobic%20petroleum.pdf"}, {"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1751-7915.13654"}, {"href": "https://eprints.ncl.ac.uk/fulltext.aspx?url=270998/E5D81B02-CB5D-4798-8D73-9D54E5057C34.pdf&pub_id=270998"}, {"href": "https://doi.org/10.1111/1751-7915.13654"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1751-7915.13654", "name": "item", "description": "10.1111/1751-7915.13654", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1751-7915.13654"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-08-30T00:00:00Z"}}, {"id": "10.1111/j.1574-6941.2007.00394.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:40Z", "type": "Journal Article", "created": "2007-10-19", "title": "Quantitation And Diversity Analysis Of Ruminal Methanogenic Populations In Response To The Antimethanogenic Compound Bromochloromethane", "description": "Methyl coenzyme-M reductase A (mcrA) clone libraries were generated from microbial DNA extracted from the rumen of cattle fed a roughage diet with and without supplementation of the antimethanogenic compound bromochloromethane. Bromochloromethane reduced total methane emissions by c. 30%, with a resultant increase in propionate and branched chain fatty acids. The mcrA clone libraries revealed that Methanobrevibacter spp. were the dominant species identified. A decrease in the incidence of Methanobrevibacter spp. from the clone library generated from bromochloromethane treatment was observed. In addition, a more diverse methanogenic population with representatives from Methanococcales, Methanomicrobiales and Methanosacinales orders was observed for the bromochloromethane library. Sequence data generated from these libraries aided in the design of an mcrA-targeted quantitative PCR (qPCR) assay. The reduction in methane production by bromochloromethane was associated with an average decrease of 34% in the number of methanogenic Archaea when monitored with this qPCR assay. Dissociation curve analysis of mcrA amplicons showed a clear difference in melting temperatures for Methanobrevibacter spp. (80-82 degrees C) and all other methanongens (84-86 degrees C). A decrease in the intensity of the Methanobrevibacter spp. specific peak and an increase for the other peak in the bromochloromethane-treated animals corresponded with the changes within the clone libraries.", "keywords": ["Male", "0301 basic medicine", "Rumen", "Bromochloromethane", "Methanogens", "Molecular Sequence Data", "Euryarchaeota", "Methanobrevibacter", "Polymerase Chain Reaction", "630", "03 medical and health sciences", "2402 Applied Microbiology and Biotechnology", "Animals", "Methyl coenzyme-M reductase", "Phylogeny", "Gene Library", "2. Zero hunger", "0303 health sciences", "Hydrocarbons", " Halogenated", "2404 Microbiology", "Sequence Analysis", " DNA", "mcrA", "qPCR", "DNA", " Archaeal", "Cattle", "Oxidoreductases", "2303 Ecology", "Methane"]}, "links": [{"href": "https://doi.org/10.1111/j.1574-6941.2007.00394.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1574-6941.2007.00394.x", "name": "item", "description": "10.1111/j.1574-6941.2007.00394.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1574-6941.2007.00394.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2007-12-01T00:00:00Z"}}, {"id": "10.1186/s13568-024-01764-7", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:19:59Z", "type": "Journal Article", "created": "2024-09-28", "title": "Metagenomic analyses of a consortium for the bioremediation of hydrocarbons polluted soils", "description": "Abstract<p>A bacterial consortium was isolated from a soil in Noblejas (Toledo, Spain) with a long history of mixed hydrocarbons pollution, by enrichment cultivation. Serial cultures of hydrocarbons polluted soil samples were grown in a minimal medium using diesel (1\uffc2\uffa0mL/L) as the sole carbon and energy source. The bacterial composition of the Noblejas Consortium (NC) was determined by sequencing 16S rRNA gene amplicon libraries. The consortium contained around 50 amplicon sequence variants (ASVs) and the major populations belonged to the genera Pseudomonas, Enterobacter, Delftia, Stenotrophomonas, Achromobacter, Acinetobacter, Novosphingobium, Allorhizobium-Neorhizobium-Rhizobium, Ochrobactrum and Luteibacter. All other genera were below 1%. Metagenomic analysis of NC has shown a high abundance of genes encoding enzymes implicated in aliphatic and (poly) aromatic hydrocarbons degradation, and almost all pathways for hydrocarbon degradation are represented. Metagenomic analysis has also allowed the construction of metagenome assembled genomes (MAGs) for the major players of NC. Metatranscriptomic analysis has shown that several of the ASVs are implicated in hydrocarbon degradation, being Pseudomonas, Acinetobacter and Delftia the most active populations.</p", "keywords": ["metagenomics", "Bacterial consortium; Bioremediation; Metagenomics; Metatranscriptomics; Total petroleum hydrocarbons", "metatranscriptomics", "Bacterial consortium", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Microbiology", "QR1-502", "Total petroleum hydrocarbons", "total petroleum hydrocarbons", "bioremediation", "Original Article", "Metagenomics", "Bioremediation", "TP248.13-248.65", "Metatranscriptomics", "Biotechnology"]}, "links": [{"href": "https://doi.org/10.1186/s13568-024-01764-7"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/AMB%20Express", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1186/s13568-024-01764-7", "name": "item", "description": "10.1186/s13568-024-01764-7", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1186/s13568-024-01764-7"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-09-28T00:00:00Z"}}, {"id": "10.13345/j.cjb.210389", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:20:06Z", "type": "Journal Article", "title": "Advances in bioremediation of polycyclic aromatic hydrocarbons contaminated soil.", "description": "Polycyclic aromatic hydrocarbons (PAHs) are a class of persistent pollutants that are widely distributed in the environment. Due to their stable structure and poor degradability, PAHs exhibit carcinogenic, teratogenic, and mutagenic toxicity to the ecological environment and organisms, thus increasing attentions have been paid to their removals and remediation. Green, safe and economical technologies are widely used in the bioremediation of PAHs-contaminated soil. This article summarizes the present status of PAHs pollution in soil of China from the aspects of origin, migration, fate, and pollution level. Meanwhile, the types of microorganisms and plants capable of degrading PAHs, as well as the underlying mechanisms, are summarized. The features of three major bioremediation technologies, i.e., microbial remediation, phytoremediation, and joint remediation, are compared. Analysis of the interaction mechanisms between plants and microorganisms, selection and cultivation of stress-resistant strains and plants, as well as safety and efficacy evaluation of practical applications, are expected to become future directions in this field.", "keywords": ["Soil", "Biodegradation", " Environmental", "Soil Pollutants", "Polycyclic Aromatic Hydrocarbons", "Soil Microbiology"], "contacts": [{"organization": "Li-Li Miao, Xi-Yan Gao, Yinghao Zhao, Meilin Zheng, Zhi-Pei Liu,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.13345/j.cjb.210389"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sheng%20wu%20gong%20cheng%20xue%20bao%20%3D%20Chinese%20journal%20of%20biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.13345/j.cjb.210389", "name": "item", "description": "10.13345/j.cjb.210389", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.13345/j.cjb.210389"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "10.2139/ssrn.4106102", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:21:06Z", "type": "Journal Article", "created": "2022-05-28", "title": "Evaluation of Biostimulation, Bioaugmentation, and Organic Amendments Application on the Bioremediation of Recalcitrant Hydrocarbons of Soil", "description": "In the present work, the operational conditions for improving the degradation rates of Total Petroleum Hydrocarbons (TPHs) in contaminated soil from a machinery park were optimized at a microcosms scale along a 90- days incubation period. In this study, bioremediation strategies and an organic amendment have been tested to verify the remediation of soil contaminated with different hydrocarbons, mineral oils, and heavy metals. Specifically, designed biostimulation and bioaugmentation strategies were compared with and without adding vermicompost. The polluted soil harboring multiple contaminants, partially attenuated for years, was used. The initial profile showed enrichment in heavy linear alkanes, suggesting a previous moderate weathering. The application of vermicompost increased five and two times the amounts of available phosphorus (P) and exchangeable potassium (K), respectively, as a direct consequence of the organic amendment addition. The microbial activity increased due to soil acidification, which influenced the solubility of P and other micronutrients. It also impacted the predominance and variability of the different microbial groups and the incubation, as reflected by phospholipid fatty acid (PLFA) results. An increase in the alkaline phosphatases and proteases linked to bacterial growth was displayed. This stimulation of microbial metabolism correlated with the degradation rates since TPHs degradation\u2019 efficiency after vermicompost addition reached 32.5% and 34.4% of the initial hydrocarbon levels for biostimulation and bioaugmentation, respectively. Although Polycyclic Aromatic", "keywords": ["Soil enzymes", "01 natural sciences", "Total petroleum hydrocarbons polluted soils", "Soil", "Bioaugmentation", "Alkanes", "Soil Pollutants", "Micronutrients", "Polycyclic Aromatic Hydrocarbons", "Materials", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences", "2. Zero hunger", "Minerals", "Materiales", "Biostimulation", " Bioaugmentation", " Phospholipid fatty acids", " Total petroleum hydrocarbons polluted soils", " Soil enzymes", "Fatty Acids", "Phosphorus", "Phenanthrenes", "Hydrocarbons", "Phosphoric Monoester Hydrolases", "6. Clean water", "Biostimulation", "Biodegradation", " Environmental", "Petroleum", "13. Climate action", "Phospholipid fatty acids", "Potassium", "Oils", "Biostimulation Bioaugmentation Phospholipid fatty acids Total petroleum hydrocarbons polluted soils Soil enzymes", "Peptide Hydrolases"]}, "links": [{"href": "https://doi.org/10.2139/ssrn.4106102"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/SSRN%20Electronic%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2139/ssrn.4106102", "name": "item", "description": "10.2139/ssrn.4106102", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2139/ssrn.4106102"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}, {"id": "10.3390/genes10060456", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:21:36Z", "type": "Journal Article", "created": "2019-06-14", "title": "Metagenomic Insights into the Bacterial Functions of a Diesel-Degrading Consortium for the Rhizoremediation of Diesel-Polluted Soil", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Diesel is a complex pollutant composed of a mixture of aliphatic and aromatic hydrocarbons. Because of this complexity, diesel bioremediation requires multiple microorganisms, which harbor the catabolic pathways to degrade the mixture. By enrichment cultivation of rhizospheric soil from a diesel-polluted site, we have isolated a bacterial consortium that can grow aerobically with diesel and different alkanes and polycyclic aromatic hydrocarbons (PAHs) as the sole carbon and energy source. Microbiome diversity analyses based on 16S rRNA gene showed that the diesel-degrading consortium consists of 76 amplicon sequence variants (ASVs) and it is dominated by Pseudomonas, Aquabacterium, Chryseobacterium, and Sphingomonadaceae. Changes in microbiome composition were observed when growing on specific hydrocarbons, reflecting that different populations degrade different hydrocarbons. Shotgun metagenome sequence analysis of the consortium growing on diesel has identified redundant genes encoding enzymes implicated in the initial oxidation of alkanes (AlkB, LadA, CYP450) and a variety of hydroxylating and ring-cleavage dioxygenases involved in aromatic and polyaromatic hydrocarbon degradation. The phylogenetic assignment of these enzymes to specific genera allowed us to model the role of specific populations in the diesel-degrading consortium. Rhizoremediation of diesel-polluted soil microcosms using the consortium, resulted in an important enhancement in the reduction of total petroleum hydrocarbons (TPHs), making it suited for rhizoremediation applications.</p></article>", "keywords": ["0301 basic medicine", "TPH", "consortium", "Article", "diesel", "03 medical and health sciences", "PAHs", "rhizoremediation", "Pseudomonas", "RNA", " Ribosomal", " 16S", "11. Sustainability", "Soil Pollutants", "Polycyclic Aromatic Hydrocarbons", "bacteria", "Phylogeny", "Soil Microbiology", "Chryseobacterium", "2. Zero hunger", "metagenomics", "rhizoremediation; diesel; bacteria; consortium; metagenomics; PAHs; TPH", "0303 health sciences", "Microbiota", "Biodiversity", "15. Life on land", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Rhizoremediation", "Biodegradation", " Environmental", "Petroleum", "13. Climate action", "Metagenome"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/10/6/456/pdf"}, {"href": "https://www.mdpi.com/2073-4425/10/6/456/pdf"}, {"href": "https://doi.org/10.3390/genes10060456"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/genes10060456", "name": "item", "description": "10.3390/genes10060456", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/genes10060456"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-14T00:00:00Z"}}, {"id": "10.3390/microorganisms9040859", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:21:40Z", "type": "Journal Article", "created": "2021-04-19", "title": "Unraveling the metabolic potential of asgardarchaeota in a sediment from the mediterranean hydrocarbon-contaminated water basin mar piccolo (Taranto, italy)", "description": "<p>Increasing number of metagenome sequencing studies have proposed a central metabolic role of still understudied Archaeal members in natural and artificial ecosystems. However, their role in hydrocarbon cycling, particularly in the anaerobic biodegradation of aliphatic and aromatic hydrocarbons, is still mostly unknown in both marine and terrestrial environments. In this work, we focused our study on the metagenomic characterization of the archaeal community inhabiting the Mar Piccolo (Taranto, Italy, central Mediterranean) sediments heavily contaminated by petroleum hydrocarbons and polychlorinated biphenyls (PCB). Among metagenomic bins reconstructed from Mar Piccolo microbial community, we have identified members of the Asgardarchaeota superphylum that has been recently proposed to play a central role in hydrocarbon cycling in natural ecosystems under anoxic conditions. In particular, we found members affiliated with Thorarchaeota, Heimdallarchaeota, and Lokiarchaeota phyla and analyzed their genomic potential involved in central metabolism and hydrocarbon biodegradation. Metabolic prediction based on metagenomic analysis identified the malonyl-CoA and benzoyl-CoA routes as the pathways involved in aliphatic and aromatic biodegradation in these Asgardarchaeota members. This is the first study to give insight into the archaeal community functionality and connection to hydrocarbon degradation in marine sediment historically contaminated by hydrocarbons.</p>", "keywords": ["<i>Lokiarchaeota</i>", "0301 basic medicine", "0303 health sciences", "QH301-705.5", "<i>Heimdallarchaeota</i>", "aromatic hydrocarbons", "<i>Thorarchaeota</i>", "Asgardarchaeota", "metagenome", "Article", "6. Clean water", "03 medical and health sciences", "bioremediation", "13. Climate action", "Mar Piccolo", "aliphatic hydrocarbons", "14. Life underwater", "Biology (General)", "Asgardarchaeota; Mar Piccolo; aromatic hydrocarbons; aliphatic hydrocarbons; Thorarchaeota; Lokiarchaeota; Heimdallarchaeota; bioremediation; metagenome"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/9/4/859/pdf"}, {"href": "https://cris.unibo.it/bitstream/11585/819019/1/microorganisms-09-00859%20%282%29.pdf"}, {"href": "https://www.mdpi.com/2076-2607/9/4/859/pdf"}, {"href": "https://doi.org/10.3390/microorganisms9040859"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms9040859", "name": "item", "description": "10.3390/microorganisms9040859", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms9040859"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-04-16T00:00:00Z"}}, {"id": "10259/9505", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-24T16:25:28Z", "type": "Journal Article", "created": "2024-03-22", "title": "Bioaugmentation and vermicompost facilitated the hydrocarbon bioremediation: scaling up from lab to field for petroleum-contaminated soils", "description": "Abstract                   <p>The biodegradation of total petroleum hydrocarbon (TPH) in soil is very challenging due to the complex recalcitrant nature of hydrocarbon, hydrophobicity, indigenous microbial adaptation and competition, and harsh environmental conditions. This work further confirmed that limited natural attenuation of petroleum hydrocarbons (TPHs) (15% removal) necessitates efficient bioremediation strategies. Hence, a scaling-up experiment for testing and optimizing the use of biopiles for bioremediation of TPH polluted soils was conducted with three 500-kg pilots of polluted soil, and respective treatments were implemented: including control soil (CT), bioaugmentation and vermicompost treatment (BAVC), and a combined application of BAVC along with bioelectrochemical snorkels (BESBAVC), all maintained at 40% field capacity. This study identified that at pilot scale level, a successful application of BAVC treatment can achieve 90.3% TPH removal after 90 days. BAVC\uffe2\uff80\uff99s effectiveness stemmed from synergistic mechanisms. Introduced microbial consortia were capable of TPH degradation, while vermicompost provided essential nutrients, enhanced aeration, and, potentially, acted as a biosorbent. Hence, it can be concluded that the combined application of BAVC significantly enhances TPH removal compared to natural attenuation. While the combined application of a bioelectrochemical snorkel (BES) with BAVC also showed a significant TPH removal, it did not differ statistically from the individual application of BAVC, under applied conditions. Further research is needed to optimize BES integration with BAVC for broader applicability. This study demonstrates BAVC as a scalable and mechanistically sound approach for TPH bioremediation in soil.</p", "keywords": ["Qu\u00edmica agr\u00edcola", "Bioqu\u00edmica", "0301 basic medicine", "vermicompost", "Passive bioelectrochemical systems", "Contaminaci\u00f3n", "passive bioelectrochemical systems", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Pollution", "Biochemistry", "01 natural sciences", "Hydrocarbons", "Advances in Environmental Biotechnology and Engineering", "Microbial consortium", "03 medical and health sciences", "Agricultural chemistry", "Bioaugmentation", "microbial consortium", "hydrocarbons", "Vermicompost", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10259/9505"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10259/9505", "name": "item", "description": "10259/9505", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10259/9505"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-03-22T00:00:00Z"}}, {"id": "10259/7423", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:25:27Z", "type": "Journal Article", "created": "2022-05-28", "title": "Evaluation of Biostimulation, Bioaugmentation, and Organic Amendments Application on the Bioremediation of Recalcitrant Hydrocarbons of Soil", "description": "In the present work, the operational conditions for improving the degradation rates of Total Petroleum Hydrocarbons (TPHs) in contaminated soil from a machinery park were optimized at a microcosms scale along a 90- days incubation period. In this study, bioremediation strategies and an organic amendment have been tested to verify the remediation of soil contaminated with different hydrocarbons, mineral oils, and heavy metals. Specifically, designed biostimulation and bioaugmentation strategies were compared with and without adding vermicompost. The polluted soil harboring multiple contaminants, partially attenuated for years, was used. The initial profile showed enrichment in heavy linear alkanes, suggesting a previous moderate weathering. The application of vermicompost increased five and two times the amounts of available phosphorus (P) and exchangeable potassium (K), respectively, as a direct consequence of the organic amendment addition. The microbial activity increased due to soil acidification, which influenced the solubility of P and other micronutrients. It also impacted the predominance and variability of the different microbial groups and the incubation, as reflected by phospholipid fatty acid (PLFA) results. An increase in the alkaline phosphatases and proteases linked to bacterial growth was displayed. This stimulation of microbial metabolism correlated with the degradation rates since TPHs degradation\u2019 efficiency after vermicompost addition reached 32.5% and 34.4% of the initial hydrocarbon levels for biostimulation and bioaugmentation, respectively. Although Polycyclic Aromatic", "keywords": ["Soil enzymes", "01 natural sciences", "Total petroleum hydrocarbons polluted soils", "Soil", "Bioaugmentation", "Alkanes", "Soil Pollutants", "Micronutrients", "Polycyclic Aromatic Hydrocarbons", "Materials", "Phospholipids", "Soil Microbiology", "0105 earth and related environmental sciences", "2. Zero hunger", "Minerals", "Materiales", "Fatty Acids", "Phosphorus", "Phenanthrenes", "Hydrocarbons", "Phosphoric Monoester Hydrolases", "6. Clean water", "Biostimulation", "Biodegradation", " Environmental", "Petroleum", "13. Climate action", "Phospholipid fatty acids", "Potassium", "Oils", "Biostimulation Bioaugmentation Phospholipid fatty acids Total petroleum hydrocarbons polluted soils Soil enzymes", "Peptide Hydrolases"]}, "links": [{"href": "https://doi.org/10259/7423"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/SSRN%20Electronic%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10259/7423", "name": "item", "description": "10259/7423", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10259/7423"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}, {"id": "10259/7490", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:25:28Z", "type": "Journal Article", "created": "2022-10-19", "title": "Comparative toxicological assessment of three soils polluted with different levels of hydrocarbons and heavy metals using in vitro and in vivo approaches", "description": "The biological effects induced by the pollutants present in soils, together with the chemical and physical characterizations, are good indicators to provide a general overview of their quality. However, the existence of studies where the toxicity associated to soils contaminated with mixtures of pollutants applying both in vitro and in vivo models are scarce. In this work, three soils (namely, Soil 001, Soil 002 and Soil 013) polluted with different concentrations of hydrocarbons and heavy metals were evaluated using different organisms representative of human (HepG2 human cell line) and environmental exposure (the yeast Saccharomyces cerevisiae, the Gram-negative bacterium Pseudomonas putida and, for the in vivo evaluation, the annelid Enchytraeus crypticus). In vitro assays showed that the soluble fraction of the Soil 001, which presented the highest levels of heavy metals, represented a great impact in the viability of the HepG2 cells and S. cerevisiae, while organic extracts from Soils 002 and 013 caused a slight decrease in the viability of HepG2 cells. In addition, in vivo experiments showed that Soils 001 and 013 affected the survival and the reproduction of E. crypticus. Altogether, these results provide a general overview of the potential hazards associated to three specific contaminated sites in a variety of organisms, showing how different concentrations of similar pollutants affect them, and highlights the relevance of testing both organic and soluble extracts when in vitro safety assays of soils are performed.", "keywords": ["Pseudomonas putida", "Enchytraeus crypticus", "Qu\u00edmica anal\u00edtica", "Saccharomyces cerevisiae", "Analytic", "01 natural sciences", "Hydrocarbons", "6. Clean water", "Chemistry", "Soil", "Soil contamination", "13. Climate action", "Metals", " Heavy", "Humans", "Soil Pollutants", "Chemistry", " Analytic", "HepG2 cells Saccharomyces cerevisiae Pseudomonas putida Enchytraeus crypticus Soil contamination", "HepG2 cells", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10259/7490"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10259/7490", "name": "item", "description": "10259/7490", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10259/7490"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-01T00:00:00Z"}}, {"id": "10261/281764", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:25:32Z", "type": "Journal Article", "created": "2022-09-14", "title": "Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media", "description": "Recent research has demonstrated that chemotactic bacteria can disperse inside microsized pores while traveling toward favorable conditions. Microbe-microbe cotransport might enable nonmotile bacteria to be carried with motile partners to enhance their dispersion and reduce their deposition in porous systems. The aim of this study was to demonstrate the enhancement in the dispersion of nonmotile bacteria (Mycobacterium gilvum VM552, a polycyclic aromatic hydrocarbon-degrader, and Sphingobium sp. D4, a hexachlorocyclohexane-degrader, through micrometer-sized pores near the exclusion-cell-size limit, in the presence of motile Pseudomonas putida G7 cells. For this purpose, we used bioreactors equipped with two chambers that were separated with membrane filters with 3, 5, and 12 \u03bcm pore sizes and capillary polydimethylsiloxane (PDMS) microarrays (20 \u03bcm \u00d7 35 \u03bcm \u00d7 2.2 mm). The cotransport of nonmotile bacteria occurred exclusively in the presence of a chemoattractant concentration gradient, and therefore, a directed flow of motile cells. This cotransport was more intense in the presence of larger pores (12 \u03bcm) and strong chemoeffectors (\u03b3-aminobutyric acid). The mechanism that governed cotransport at the cell scale involved mechanical pushing and hydrodynamic interactions. Chemotaxis-mediated cotransport of bacterial degraders and its implications in pore accessibility opens new avenues for the enhancement of bacterial dispersion in porous media and the biodegradation of heterogeneously contaminated scenarios.", "keywords": ["Chemotactic Factors", "Pseudomonas putida", "Chemotaxis", "Bioaccessibility", "01 natural sciences", "Microbe-microbe cotransport", "Dimethylpolysiloxanes", "Polycyclic Aromatic Hydrocarbons", "Micrometer-sized pores", "Porosity", "Hexachlorocyclohexane", "gamma-Aminobutyric Acid", "Hitchhiking", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.2c03149"}, {"href": "https://doi.org/10261/281764"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10261/281764", "name": "item", "description": "10261/281764", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10261/281764"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-09-14T00:00:00Z"}}, {"id": "10486/713957", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:25:42Z", "type": "Journal Article", "created": "2019-06-14", "title": "Metagenomic Insights into the Bacterial Functions of a Diesel-Degrading Consortium for the Rhizoremediation of Diesel-Polluted Soil", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Diesel is a complex pollutant composed of a mixture of aliphatic and aromatic hydrocarbons. Because of this complexity, diesel bioremediation requires multiple microorganisms, which harbor the catabolic pathways to degrade the mixture. By enrichment cultivation of rhizospheric soil from a diesel-polluted site, we have isolated a bacterial consortium that can grow aerobically with diesel and different alkanes and polycyclic aromatic hydrocarbons (PAHs) as the sole carbon and energy source. Microbiome diversity analyses based on 16S rRNA gene showed that the diesel-degrading consortium consists of 76 amplicon sequence variants (ASVs) and it is dominated by Pseudomonas, Aquabacterium, Chryseobacterium, and Sphingomonadaceae. Changes in microbiome composition were observed when growing on specific hydrocarbons, reflecting that different populations degrade different hydrocarbons. Shotgun metagenome sequence analysis of the consortium growing on diesel has identified redundant genes encoding enzymes implicated in the initial oxidation of alkanes (AlkB, LadA, CYP450) and a variety of hydroxylating and ring-cleavage dioxygenases involved in aromatic and polyaromatic hydrocarbon degradation. The phylogenetic assignment of these enzymes to specific genera allowed us to model the role of specific populations in the diesel-degrading consortium. Rhizoremediation of diesel-polluted soil microcosms using the consortium, resulted in an important enhancement in the reduction of total petroleum hydrocarbons (TPHs), making it suited for rhizoremediation applications.</p></article>", "keywords": ["0301 basic medicine", "TPH", "consortium", "Article", "diesel", "03 medical and health sciences", "PAHs", "rhizoremediation", "Pseudomonas", "RNA", " Ribosomal", " 16S", "11. Sustainability", "Soil Pollutants", "Polycyclic Aromatic Hydrocarbons", "bacteria", "Phylogeny", "Soil Microbiology", "Chryseobacterium", "2. Zero hunger", "metagenomics", "rhizoremediation; diesel; bacteria; consortium; metagenomics; PAHs; TPH", "0303 health sciences", "Microbiota", "Biodiversity", "15. Life on land", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Rhizoremediation", "Biodegradation", " Environmental", "Petroleum", "13. Climate action", "Metagenome"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/10/6/456/pdf"}, {"href": "https://www.mdpi.com/2073-4425/10/6/456/pdf"}, {"href": "https://doi.org/10486/713957"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10486/713957", "name": "item", "description": "10486/713957", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10486/713957"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-14T00:00:00Z"}}, {"id": "10486/717833", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-24T16:25:42Z", "type": "Journal Article", "created": "2024-03-22", "title": "Bioaugmentation and vermicompost facilitated the hydrocarbon bioremediation: scaling up from lab to field for petroleum-contaminated soils", "description": "Abstract           <p>The biodegradation of total petroleum hydrocarbon (TPH) in soil is very challenging due to the complex recalcitrant nature of hydrocarbon, hydrophobicity, indigenous microbial adaptation and competition, and harsh environmental conditions. This work further confirmed that limited natural attenuation of petroleum hydrocarbons (TPHs) (15% removal) necessitates efficient bioremediation strategies. Hence, a scaling-up experiment for testing and optimizing the use of biopiles for bioremediation of TPH polluted soils was conducted with three 500-kg pilots of polluted soil, and respective treatments were implemented: including control soil (CT), bioaugmentation and vermicompost treatment (BAVC), and a combined application of BAVC along with bioelectrochemical snorkels (BESBAVC), all maintained at 40% field capacity. This study identified that at pilot scale level, a successful application of BAVC treatment can achieve 90.3% TPH removal after 90 days. BAVC\uffe2\uff80\uff99s effectiveness stemmed from synergistic mechanisms. Introduced microbial consortia were capable of TPH degradation, while vermicompost provided essential nutrients, enhanced aeration, and, potentially, acted as a biosorbent. Hence, it can be concluded that the combined application of BAVC significantly enhances TPH removal compared to natural attenuation. While the combined application of a bioelectrochemical snorkel (BES) with BAVC also showed a significant TPH removal, it did not differ statistically from the individual application of BAVC, under applied conditions. Further research is needed to optimize BES integration with BAVC for broader applicability. This study demonstrates BAVC as a scalable and mechanistically sound approach for TPH bioremediation in soil.</p", "keywords": ["Qu\u00edmica agr\u00edcola", "Bioqu\u00edmica", "0301 basic medicine", "vermicompost", "Passive bioelectrochemical systems", "Contaminaci\u00f3n", "passive bioelectrochemical systems", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Pollution", "Biochemistry", "01 natural sciences", "Hydrocarbons", "Microbial consortium", "03 medical and health sciences", "Bioaugmentation", "Agricultural chemistry", "microbial consortium", "hydrocarbons", "Vermicompost", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10486/717833"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10486/717833", "name": "item", "description": "10486/717833", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10486/717833"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-03-22T00:00:00Z"}}, {"id": "10486/717838", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:25:42Z", "type": "Journal Article", "created": "2024-09-28", "title": "Metagenomic analyses of a consortium for the bioremediation of hydrocarbons polluted soils", "description": "Abstract<p>A bacterial consortium was isolated from a soil in Noblejas (Toledo, Spain) with a long history of mixed hydrocarbons pollution, by enrichment cultivation. Serial cultures of hydrocarbons polluted soil samples were grown in a minimal medium using diesel (1\uffc2\uffa0mL/L) as the sole carbon and energy source. The bacterial composition of the Noblejas Consortium (NC) was determined by sequencing 16S rRNA gene amplicon libraries. The consortium contained around 50 amplicon sequence variants (ASVs) and the major populations belonged to the genera Pseudomonas, Enterobacter, Delftia, Stenotrophomonas, Achromobacter, Acinetobacter, Novosphingobium, Allorhizobium-Neorhizobium-Rhizobium, Ochrobactrum and Luteibacter. All other genera were below 1%. Metagenomic analysis of NC has shown a high abundance of genes encoding enzymes implicated in aliphatic and (poly) aromatic hydrocarbons degradation, and almost all pathways for hydrocarbon degradation are represented. Metagenomic analysis has also allowed the construction of metagenome assembled genomes (MAGs) for the major players of NC. Metatranscriptomic analysis has shown that several of the ASVs are implicated in hydrocarbon degradation, being Pseudomonas, Acinetobacter and Delftia the most active populations.</p", "keywords": ["metagenomics", "Bacterial consortium; Bioremediation; Metagenomics; Metatranscriptomics; Total petroleum hydrocarbons", "metatranscriptomics", "Bacterial consortium", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Microbiology", "QR1-502", "Total petroleum hydrocarbons", "total petroleum hydrocarbons", "bioremediation", "Original Article", "Metagenomics", "Bioremediation", "TP248.13-248.65", "Metatranscriptomics", "Biotechnology"]}, "links": [{"href": "https://doi.org/10486/717838"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/AMB%20Express", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10486/717838", "name": "item", "description": "10486/717838", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10486/717838"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-09-28T00:00:00Z"}}, {"id": "11104/0309544", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:25:48Z", "type": "Journal Article", "created": "2020-04-02", "title": "Analysis of the biodegradative and adaptive potential of the novel polychlorinated biphenyl degrader Rhodococcus sp. WAY2 revealed by its complete genome sequence", "description": "<p>The complete genome sequence of                                                Rhodococcus                                          sp. WAY2 (WAY2) consists of a circular chromosome, three linear replicons and a small circular plasmid. The linear replicons contain typical actinobacterial invertron-type telomeres with the central CGTXCGC motif. Comparative phylogenetic analysis of the 16S rRNA gene along with phylogenomic analysis based on the genome-to-genome blast distance phylogeny (GBDP) algorithm and digital DNA\uffe2\uff80\uff93DNA hybridization (dDDH) with other                                                Rhodococcus                                          type strains resulted in a clear differentiation of WAY2, which is likely a new species. The genome of WAY2 contains five distinct clusters of bph, etb and nah genes, putatively involved in the degradation of several aromatic compounds. These clusters are distributed throughout the linear plasmids. The high sequence homology of the ring-hydroxylating subunits of these systems with other known enzymes has allowed us to model the range of aromatic substrates they could degrade. Further functional characterization revealed that WAY2 was able to grow with biphenyl, naphthalene and xylene as sole carbon and energy sources, and could oxidize multiple aromatic compounds, including ethylbenzene, phenanthrene, dibenzofuran and toluene. In addition, WAY2 was able to co-metabolize 23 polychlorinated biphenyl congeners, consistent with the five different ring-hydroxylating systems encoded by its genome. WAY2 could also use n-alkanes of various chain-lengths as a sole carbon source, probably due to the presence of alkB and ladA gene copies, which are only found in its chromosome. These results show that WAY2 has a potential to be used for the biodegradation of multiple organic compounds.</p", "keywords": ["0301 basic medicine", "0303 health sciences", "PCB", "Whole Genome Sequencing", "AlkB Enzymes", "High-Throughput Nucleotide Sequencing", "PAH", "Naphthalenes", "Xylenes", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "biodegradation", "Polychlorinated Biphenyls", "Hydrocarbons", "Complete genome", "03 medical and health sciences", "Biodegradation", " Environmental", "RNA", " Ribosomal", " 16S", "Biodegradation", "Cluster Analysis", "Rhodococcus", "Phylogeny", "Research Article"]}, "links": [{"href": "https://doi.org/11104/0309544"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Genomics", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11104/0309544", "name": "item", "description": "11104/0309544", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11104/0309544"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-01T00:00:00Z"}}, {"id": "11245.1/7f8528e3-827f-4f02-91e5-87e1119aa5c1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:25:49Z", "type": "Journal Article", "created": "2023-01-18", "title": "Risk reductions during pyrene biotransformation and mobilization in a model plant-bacteria-biochar system", "description": "The productive application of motile microorganisms for degrading hydrophobic contaminants in soil is one of the most promising processes in modern remediation due to its sustainability and low cost. However, the incomplete biodegradation of the contaminants and the formation of the intermediary metabolites in the process may increase the toxicity in soil during bioremediation, and motile inoculants may mobilize the pollutants through biosorption. Therefore, controlling these factors should be a fundamental part of soil remediation approaches. The aim of this study was to evaluate the sources of risk associated with the cometabolism-based transformation of 14C-labeled pyrene by inoculated Pseudomonas putida G7 and identify ways to minimize risk. Our model scenario examined the increase in bioaccessibility to a distant source of contamination facilitated by sunflower (Helianthus annuus L.) roots. A biochar trap for mobilized pollutant metabolites and bacteria has also been employed. The experimental design consisted of pots filled with a layer of sand with 14C-labeled pyrene (88 mg kg-1) as a contamination focus located several centimeters from the inoculation point. Half of the pots included a biochar layer at the bottom. The pots were incubated in a greenhouse with sunflower plants and P. putida G7 bacteria. Pots with sunflower plants showed a higher biodegradation of pyrene, its mobilization as metabolites through the percolate and the roots, and bacterial mobilization toward the source of contamination, also resulting in increased pyrene transformation. In addition, the biochar layer efficiently reduced the concentrations of pyrene metabolites collected in the leachates. Therefore, the combination of plants, motile bacteria and biochar safely reduced the risk caused by the biological transformation of pyrene.", "keywords": ["Risk", "2. Zero hunger", "570", "Pyrenes", "Bacteria", "04 agricultural and veterinary sciences", "Plants", "01 natural sciences", "Polycyclic aromatic hydrocarbons", "6. Clean water", "Sunflower", "Soil", "Biodegradation", " Environmental", "13. Climate action", "Biodegradation", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Polycyclic Aromatic Hydrocarbons", "Bioremediation", "Biotransformation", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/11245.1/7f8528e3-827f-4f02-91e5-87e1119aa5c1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11245.1/7f8528e3-827f-4f02-91e5-87e1119aa5c1", "name": "item", "description": "11245.1/7f8528e3-827f-4f02-91e5-87e1119aa5c1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11245.1/7f8528e3-827f-4f02-91e5-87e1119aa5c1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-04-01T00:00:00Z"}}, {"id": "2614218019", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:26:46Z", "type": "Journal Article", "created": "2017-05-16", "title": "Nontarget Analysis Reveals a Bacterial Metabolite of Pyrene Implicated in the Genotoxicity of Contaminated Soil after Bioremediation", "description": "Bioremediation is an accepted technology for cleanup of soil contaminated with polycyclic aromatic hydrocarbons (PAHs), but it can increase the genotoxicity of the soil despite removal of the regulated PAHs. Although polar biotransformation products have been implicated as causative genotoxic agents, no specific product has been identified. We pursued a nontarget analytical approach combining effect-directed analysis (EDA) and metabolite profiling to compare extracts of PAH-contaminated soil from a former manufactured-gas plant site before and after treatment in a laboratory-scale aerobic bioreactor. A compound with the composition C15H8O2 and four methylated homologues were shown to accumulate as a result of bioreactor treatment, and the C15H8O2 compound purified from soil extracts was determined to be genotoxic. Its structure was established by nuclear magnetic resonance and mass spectroscopy as a heretofore unidentified \u03b1,\u03b2-unsaturated lactone derived from dioxygenation of pyrene at an apical ring, 2H-naphtho[2,1,8-def]chromen-2-one (NCO), which was confirmed by synthesis. The concentration of NCO in the bioreactor was 11 \u03bcg g-1 dry soil, corresponding to 13% of the pyrene removed. It also accumulated in aerobically incubated soil from two additional PAH-contaminated sites and was formed from pyrene by two pyrene-degrading bacterial cultures known to be geographically widespread, underscoring its potential environmental significance.", "keywords": ["2. Zero hunger", "Soil", "Biodegradation", " Environmental", "Pyrenes", "13. Climate action", "Soil Pollutants", "Polycyclic Aromatic Hydrocarbons", "01 natural sciences", "Soil Microbiology", "6. Clean water", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.7b01172"}, {"href": "https://doi.org/2614218019"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2614218019", "name": "item", "description": "2614218019", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2614218019"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-05-30T00:00:00Z"}}, {"id": "2770073313", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:26:48Z", "type": "Journal Article", "created": "2017-11-21", "title": "Diversity and Abundance of High-Molecular-Weight Azaarenes in PAH-Contaminated Environmental Samples", "description": "Azaarenes are N-heterocyclic polyaromatic pollutants that co-occur with polycyclic aromatic hydrocarbons (PAHs) in contaminated soils. Despite the known toxicity of some high-molecular-weight azaarenes, their diversity, abundance, and fate in contaminated soils remain to be elucidated. We applied high-resolution mass spectrometry and mass-defect filtering to four PAH-contaminated samples from geographically distant sites and detected 232 azaarene congeners distributed in eight homologous series, including alkylated derivatives and two hitherto unknown series. Four- and five-ring azaarenes were detected among these series, and the most abundant nonalkylated congeners groups (C13H9N, C15H9N, C17H11N, C19H11N, and C21H13N) were quantified. The profiles of congener groups varied among different sites. Three-ring azaarenes presented higher concentrations in unweathered sites, while four- and five-ring azaarenes predominated in weathered sites. Known toxic and carcinogenic azaarenes, such as benzo[c]acridine and dibenzo[a,h]acridine, were detected along with their multiple isomers. Our results highlight a previously unrecognized diversity and abundance of azaarenes in PAH-contaminated sites, with corresponding implications for environmental monitoring and risk assessment.", "keywords": ["13. Climate action", "11. Sustainability", "Polycyclic Aromatic Hydrocarbons", "Risk Assessment", "01 natural sciences", "Environmental Monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.7b03319"}, {"href": "https://doi.org/2770073313"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2770073313", "name": "item", "description": "2770073313", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2770073313"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-12-01T00:00:00Z"}}, {"id": "2792552733", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:26:49Z", "type": "Journal Article", "created": "2018-03-18", "title": "Comparison of theory and experiment for NAPL dissolution in porous media", "description": "Contamination of groundwater resources by an immiscible organic phase commonly called NAPL (Non Aqueous Phase Liquid) represents a major scientific challenge considering the residence time of such a pollutant. This contamination leads to the formation of NAPL blobs trapped in the soil and impact of this residual saturation cannot be ignored for correct predictions of the contaminant fate. In this paper, we present results of micromodel experiments on the dissolution of pure hydrocarbon phase (toluene). They were conducted for two values of the P\u00e9clet number. These experiments provide data for comparison and validation of a two-phase non-equilibrium theoretical model developed by Quintard and Whitaker (1994) using the volume averaging method. The model was directly upscaled from the averaged pore-scale mass balance equations. The effective properties of the macroscopic model were calculated over periodic unit cells designed from images of the experimental flow cell. Comparison of experimental and numerical results shows that the transport model predicts correctly - with no fitting parameters - the main mechanisms of NAPL mass transfer. The study highlights the crucial need of having a fair recovery of pore-scale characteristic lengths to predict the mass transfer coefficient with accuracy.", "keywords": ["0208 environmental biotechnology", "0207 environmental engineering", "02 engineering and technology", "Models", " Theoretical", "Hydrocarbons", "6. Clean water", "Soil", "Solubility", "13. Climate action", "Hydrology", "Groundwater", "Porosity", "Water Pollutants", " Chemical", "Toluene"]}, "links": [{"href": "https://doi.org/2792552733"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Contaminant%20Hydrology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2792552733", "name": "item", "description": "2792552733", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2792552733"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-04-01T00:00:00Z"}}, {"id": "29573829", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:26:56Z", "type": "Journal Article", "created": "2018-03-18", "title": "Comparison of theory and experiment for NAPL dissolution in porous media", "description": "Contamination of groundwater resources by an immiscible organic phase commonly called NAPL (Non Aqueous Phase Liquid) represents a major scientific challenge considering the residence time of such a pollutant. This contamination leads to the formation of NAPL blobs trapped in the soil and impact of this residual saturation cannot be ignored for correct predictions of the contaminant fate. In this paper, we present results of micromodel experiments on the dissolution of pure hydrocarbon phase (toluene). They were conducted for two values of the P\u00e9clet number. These experiments provide data for comparison and validation of a two-phase non-equilibrium theoretical model developed by Quintard and Whitaker (1994) using the volume averaging method. The model was directly upscaled from the averaged pore-scale mass balance equations. The effective properties of the macroscopic model were calculated over periodic unit cells designed from images of the experimental flow cell. Comparison of experimental and numerical results shows that the transport model predicts correctly - with no fitting parameters - the main mechanisms of NAPL mass transfer. The study highlights the crucial need of having a fair recovery of pore-scale characteristic lengths to predict the mass transfer coefficient with accuracy.", "keywords": ["Volume averaging method", "0208 environmental biotechnology", "Porous media", "0207 environmental engineering", "[SPI.MECA.MEFL] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Fluids mechanics [physics.class-ph]", "[SPI.FLUID] Engineering Sciences [physics]/Reactive fluid environment", "02 engineering and technology", "Models", " Theoretical", "Porous media flow", "Hydrocarbons", "6. Clean water", "Soil", "Solubility", "Upscaling transport", "13. Climate action", "Volume Averaging", "Upscaling", "[SDU.STU.HY] Sciences of the Universe [physics]/Earth Sciences/Hydrology", "NAPL dissolution", "Hydrology", "Groundwater", "Porosity", "Water Pollutants", " Chemical", "Toluene"]}, "links": [{"href": "https://doi.org/29573829"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Contaminant%20Hydrology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "29573829", "name": "item", "description": "29573829", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/29573829"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-04-01T00:00:00Z"}}, {"id": "2950940967", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:26:56Z", "type": "Journal Article", "created": "2019-06-14", "title": "Metagenomic Insights into the Bacterial Functions of a Diesel-Degrading Consortium for the Rhizoremediation of Diesel-Polluted Soil", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Diesel is a complex pollutant composed of a mixture of aliphatic and aromatic hydrocarbons. Because of this complexity, diesel bioremediation requires multiple microorganisms, which harbor the catabolic pathways to degrade the mixture. By enrichment cultivation of rhizospheric soil from a diesel-polluted site, we have isolated a bacterial consortium that can grow aerobically with diesel and different alkanes and polycyclic aromatic hydrocarbons (PAHs) as the sole carbon and energy source. Microbiome diversity analyses based on 16S rRNA gene showed that the diesel-degrading consortium consists of 76 amplicon sequence variants (ASVs) and it is dominated by Pseudomonas, Aquabacterium, Chryseobacterium, and Sphingomonadaceae. Changes in microbiome composition were observed when growing on specific hydrocarbons, reflecting that different populations degrade different hydrocarbons. Shotgun metagenome sequence analysis of the consortium growing on diesel has identified redundant genes encoding enzymes implicated in the initial oxidation of alkanes (AlkB, LadA, CYP450) and a variety of hydroxylating and ring-cleavage dioxygenases involved in aromatic and polyaromatic hydrocarbon degradation. The phylogenetic assignment of these enzymes to specific genera allowed us to model the role of specific populations in the diesel-degrading consortium. Rhizoremediation of diesel-polluted soil microcosms using the consortium, resulted in an important enhancement in the reduction of total petroleum hydrocarbons (TPHs), making it suited for rhizoremediation applications.</p></article>", "keywords": ["0301 basic medicine", "TPH", "consortium", "Article", "diesel", "03 medical and health sciences", "PAHs", "rhizoremediation", "Pseudomonas", "RNA", " Ribosomal", " 16S", "11. Sustainability", "Soil Pollutants", "Polycyclic Aromatic Hydrocarbons", "bacteria", "Phylogeny", "Soil Microbiology", "Chryseobacterium", "2. Zero hunger", "metagenomics", "rhizoremediation; diesel; bacteria; consortium; metagenomics; PAHs; TPH", "0303 health sciences", "Microbiota", "Biodiversity", "15. Life on land", "Biolog\u00eda y Biomedicina / Biolog\u00eda", "Rhizoremediation", "Biodegradation", " Environmental", "Petroleum", "13. Climate action", "Metagenome"]}, "links": [{"href": "http://www.mdpi.com/2073-4425/10/6/456/pdf"}, {"href": "https://www.mdpi.com/2073-4425/10/6/456/pdf"}, {"href": "https://doi.org/2950940967"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2950940967", "name": "item", "description": "2950940967", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2950940967"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-14T00:00:00Z"}}, {"id": "2987299445", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:26:58Z", "type": "Journal Article", "created": "2019-11-17", "title": "Isomer-selective biodegradation of high-molecular-weight azaarenes in PAH-contaminated environmental samples", "description": "Polycyclic aromatic nitrogen heterocycles, or azaarenes, normally co-occur with polycyclic aromatic hydrocarbons (PAHs) in contaminated soils. We recently reported that nontarget analysis using high resolution mass spectrometry of samples from four PAH-contaminated sites revealed a previously unrecognized diversity and abundance of azaarene isomers and their methylated derivatives. Here we evaluated their biodegradability by natural microbial communities from each site in aerobic microcosm incubations under biostimulated conditions. The removal of total quantifiable azaarenes ranged from 15 to 85%, and was related to the initial degree of weathering for each sample. While three-ring azaarenes were readily biodegradable, the five-ring congeners were the most recalcitrant. Microbial-mediated removal of four-ring congeners varied for different isomers, which might be attributed to the position of the nitrogen atom that also influences the physicochemical properties of azaarenes and possibly the susceptibility to transformation by relevant microbial enzymes. The presence of methyl groups also influenced azaarene biodegradability, which decreased with increasing degree of methylation. Several oxidation products of azaarenes were detected, including ketones and dioxygenated derivatives of three- and four-ring compounds. Our results indicate the susceptibility of some azaarenes to bioremediation, while suggesting the potential implications for risk from the persistence of less-biodegradable isomers and the formation of oxidized-azaarene derivatives.", "keywords": ["0301 basic medicine", "03 medical and health sciences", "Biodegradation", " Environmental", "13. Climate action", "Soil Pollutants", "Polycyclic Aromatic Hydrocarbons", "01 natural sciences", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/2987299445"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2987299445", "name": "item", "description": "2987299445", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2987299445"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-01T00:00:00Z"}}, {"id": "39584593", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:27:38Z", "type": "Journal Article", "created": "2024-11-25", "title": "Application of herbaceous plant mixtures for remediation of TPH-contaminated soil", "description": "Soil pollution with petroleum products is an urgent public health and environmental problem. Therefore, innovative solutions for cleaning soils contaminated with petroleum products are needed. One such solution is rhizodegradation, which is recognized as a sustainable and effective method of in situ soil remediation. Much of the previous research was done with monocultures, therefore the effects of different combinations of plants on the removal of petroleum products remain ambiguous. These studies evaluated three different herbaceous plant mixtures for the removal of total petroleum hydrocarbons (TPHs) from contaminated soil. Promising results were obtained. Selected herbaceous plant species and their mixtures can be successfully grown in contaminated soil at a contamination level of 6,817\u2009mg/kg TPH DW according to the selected cultivation strategy. After applying a complex of biotechnology and agronomic solutions, the morphological and morphometric indicators revealed the good adaptability and tolerance of the selected herbaceous plants to growing in contaminated soil. After two years of pot testing application of different mixtures of herbaceous plants, the TPH (C6-C40) removal potential reached 85-90%.", "keywords": ["Soil", "Biodegradation", " Environmental", "Petroleum", "Soil Pollutants", "Hydrocarbons", "Environmental Restoration and Remediation"]}, "links": [{"href": "https://doi.org/39584593"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Phytoremediation", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "39584593", "name": "item", "description": "39584593", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/39584593"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-11-25T00:00:00Z"}}, {"id": "39694292", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-24T16:27:38Z", "type": "Journal Article", "created": "2024-12-20", "title": "PFAS (per- and polyfluorinated alkyl substances) as EDCs (endocrine-disrupting chemicals) - Identification of compounds with high potential to bind to selected terpenoids NHRs (nuclear hormone receptors)", "description": "The file contains a publication entitled \u2018PFAS (per- and polyfluorinated alkyl substances) as EDCs (endocrine-disrupting chemicals) - Identification of compounds with high potential to bind to selected terpenoids NHRs (nuclear hormone receptors)\u2019 by Natalia Bulawska, Anita Sosnowska, Dominika Kowalska, Maciej St\u0119pnik, Tomasz Puzyn with Supplementary Materials.", "keywords": ["Molecular Docking Simulation", "Fluorocarbons", "Hydrocarbons", " Fluorinated", "Quantitative Structure-Activity Relationship", "Receptors", " Cytoplasmic and Nuclear", "Humans", "Endocrine Disruptors"]}, "links": [{"href": "https://doi.org/39694292"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "39694292", "name": "item", "description": "39694292", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/39694292"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-02-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Hydrocarbons&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Hydrocarbons&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Hydrocarbons&", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Hydrocarbons&offset=50", "hreflang": "en-US"}], "numberMatched": 57, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-06-25T09:24:19.166653Z"}