{"type": "FeatureCollection", "features": [{"id": "10.1126/sciadv.aas9024", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:19:18Z", "type": "Journal Article", "created": "2018-07-25", "title": "Biodegradation of synthetic polymers in soils: Tracking carbon into CO             2             and microbial biomass", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Stable isotope labeling of agricultural polyesters enables demonstration of their microbial utilization in soils.</p></article>", "keywords": ["0301 basic medicine", "Polymers", "Polyesters", "Spectrometry", " Mass", " Secondary Ion", "ENZYMATIC-HYDROLYSIS", "CATERPILLARS", "ECOLOGY", "7. Clean energy", "03 medical and health sciences", "Biomass", "SDG 2 \u2013 Kein Hunger", "SDG 2 - Zero Hunger", "POLYESTERS", "Research Articles", "Soil Microbiology", "2. Zero hunger", "106022 Mikrobiologie", "Carbon Isotopes", "0303 health sciences", "Fungi", "Agriculture", "Lipase", "Carbon Dioxide", "Carbon", "COPOLYESTERS", "Biodegradation", " Environmental", "13. Climate action", "106022 Microbiology", "POLYETHYLENE BIO-DEGRADATION"]}, "links": [{"href": "https://doi.org/10.1126/sciadv.aas9024"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20Advances", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1126/sciadv.aas9024", "name": "item", "description": "10.1126/sciadv.aas9024", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1126/sciadv.aas9024"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-06T00:00:00Z"}}, {"id": "10.1007/s11356-019-06822-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:13Z", "type": "Journal Article", "created": "2019-12-02", "title": "Effect of alkaline pretreatments on the enzymatic hydrolysis of wheat straw", "description": "Lignocellulosic materials are mainly consisted of lignin, cellulose, and hemicellulose. Lignin is recognized as the main obstacle for the enzymatic saccharification of cellulose towards the fermentable sugars' production. Hence, the removal of lignin from the lignocellulosic feedstock is beneficial for reducing the recalcitrance of lignocellulose for enzymatic attack. For this purpose, various different alkaline pretreatments were examined in order to study their effect on the enzymatic saccharification of wheat straw, as a typical lignocellulosic material. Results revealed that the alkaline pretreatments promoted delignification reactions. Regarding the removal of lignin, the most efficient pretreatments were alkaline treatment with hydrogen peroxide 10% and NaOH 2% autoclave with delignification efficiencies of 89.60% and 84.86% respectively. X-ray diffraction analysis was performed to enlighten the structural changes of raw and pretreated materials. The higher the delignification of the raw material, the higher the conversion of cellulose during enzymatic saccharification. In all cases after enzymatic saccharification, the cellulosic conversion was much higher (32-77%) than the untreated wheat straw (8.6%). After undergoing alkaline peroxide 10% pretreatment and cellulase treatment, 99% of the initial raw straw was eventually solubilized. Thus, wheat straw could be considered as an ideal material for the production of glucose with proper pretreatments and effective enzymatic hydrolysis.", "keywords": ["0106 biological sciences", "Cellulase", "Polysaccharides", "Hydrolysis", "Cellulose", "Lignin", "01 natural sciences", "Triticum", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s11356-019-06822-3.pdf"}, {"href": "https://doi.org/10.1007/s11356-019-06822-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11356-019-06822-3", "name": "item", "description": "10.1007/s11356-019-06822-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11356-019-06822-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-12-01T00:00:00Z"}}, {"id": "10.1007/s11356-020-08905-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:13Z", "type": "Journal Article", "created": "2020-04-27", "title": "Towards upscaling the valorization of wheat straw residues: alkaline pretreatment using sodium hydroxide, enzymatic hydrolysis and biogas production", "description": "Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the development of anaerobic digestion technology. The physical and chemical characteristics of wheat straw (WS), as a representative lignocellulosic biomass, have a significant impact on the anaerobic digestion process in terms of quantity and quality of the produced biogas. This study aimed at investigating the enzymatic saccharification and detoxification of straw prior to anaerobic digestion with the final objective of enhancing the performance of conventional anaerobic systems of recalcitrant fractions of agricultural waste. The experimental activity was performed in lab and pilot scale treating WS. Alkaline delignification of straw using sodium hydroxide (NaOH) was studied prior to enzymatic hydrolysis for the production of easily biodegradable sugars. After defining the optimum conditions for the pretreatment scheme, the anaerobic digestability of the effluents produced was measured. Finally, the final liquid effluents were fed to a pilot scale anaerobic digester of 0.5\u00a0m3 volume, applying an increasing organic loading rate (OLR) regime (in terms of chemical oxygen demand (COD) from 0.2 to 15\u00a0kg COD/m3/day). The optimum conditions for the delignification and enzymatic hydrolysis of WS were defined as 0.5\u00a0M NaOH at 50\u00a0\u00b0C for 3-5\u00a0h and 15\u00a0\u03bcL Cellic CTec2/g pretreated straw at 50\u00a0\u00b0C. It was proven that the resulting liquid effluents could be fed to an anaerobic digester in the ratio that they are produced with satisfactory COD removal efficiencies (over 70%) for OLRs up to 10\u00a0kg COD/m3/day. This value is correspondent to a hydraulic retention time of around 7.5\u00a0days, much lower than the respective one for untreated straw (over 12\u00a0days).", "keywords": ["2. Zero hunger", "Hydrolysis", "0211 other engineering and technologies", "02 engineering and technology", "7. Clean energy", "6. Clean water", "12. Responsible consumption", "Biofuels", "0202 electrical engineering", " electronic engineering", " information engineering", "Sodium Hydroxide", "Anaerobiosis", "Biomass", "Methane", "Triticum"]}, "links": [{"href": "https://link.springer.com/content/pdf/10.1007/s11356-020-08905-y.pdf"}, {"href": "https://doi.org/10.1007/s11356-020-08905-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11356-020-08905-y", "name": "item", "description": "10.1007/s11356-020-08905-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11356-020-08905-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-04-28T00:00:00Z"}}, {"id": "10.1016/j.biortech.2012.01.120", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:49Z", "type": "Journal Article", "created": "2012-02-03", "title": "Chemical And Biochemical Characterisation Of Biochar-Blended Composts Prepared From Poultry Manure", "description": "The aim of this study was to assess the effect of a 2% (v/v) addition of biochar on the quality of a composting mixture prepared with poultry manure and different local organic wastes (rice husk and apple pomace). Compost quality was evaluated in terms of typical stabilisation indices, the microbial biomass and selected enzymatic activities related to the C, N and P cycles. The main effects of biochar were a 10% increase in C captured by humic substance extraction and a 30% decrease of water-soluble C, due to an enhanced degradation rate and/or the sorption of these labile compounds into the biochar. The urease, phosphatase and polyphenol oxidase activities of the biochar-blended compost were enhanced by 30-40% despite the lower amount of microbial biomass. Denaturing gradient gel electrophoresis revealed a higher diversity of fungi in biochar-amended compost, suggesting a change in microbial composition compared to the unamended compost.", "keywords": ["2. Zero hunger", "Hydrolysis", "04 agricultural and veterinary sciences", "Urease", "01 natural sciences", "Phosphoric Monoester Hydrolases", "Poultry", "Manure", "Soil", "Animals", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Catechol Oxidase", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.biortech.2012.01.120"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biortech.2012.01.120", "name": "item", "description": "10.1016/j.biortech.2012.01.120", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biortech.2012.01.120"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-04-01T00:00:00Z"}}, {"id": "10.1016/j.biortech.2017.03.095", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:49Z", "type": "Journal Article", "created": "2017-03-20", "title": "Hydrothermal treatment followed by enzymatic hydrolysis and hydrothermal carbonization as means to valorise agro- and forest-based biomass residues", "description": "The suitability of several abundant but underutilized agro and forest based biomass residues for hydrothermal treatment followed by enzymatic hydrolysis as well as for hydrothermal carbonization was studied. The selected approaches represent simple biotechnical and thermochemical treatment routes suitable for wet biomass. Based on the results, the hydrothermal pre-treatment followed by enzymatic hydrolysis seemed to be most suitable for processing of carbohydrate rich corn leaves, corn stover, wheat straw and willow. High content of thermally stable components (i.e. lignin) and low content of ash in the biomass were advantageous for hydrothermal carbonization of grape pomace, coffee cake, Scots pine bark and willow.", "keywords": ["2. Zero hunger", "biomass", "Hydrolysis", "ta220", "0211 other engineering and technologies", "02 engineering and technology", "Forests", "15. Life on land", "enzyme hydrolysis", "Lignin", "Zea mays", "01 natural sciences", "7. Clean energy", "hydrothermal carbonization", "hydrothermal treatment", "lignocellulose", "13. Climate action", "ta219", "SDG 7 - Affordable and Clean Energy", "Biomass", "ta218", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.biortech.2017.03.095"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biortech.2017.03.095", "name": "item", "description": "10.1016/j.biortech.2017.03.095", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biortech.2017.03.095"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-07-01T00:00:00Z"}}, {"id": "10.1016/j.jenvman.2018.01.064", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:16:38Z", "type": "Journal Article", "created": "2018-02-05", "title": "Elemental sulfur-based autotrophic denitrification and denitritation: microbially catalyzed sulfur hydrolysis and nitrogen conversions", "description": "The hydrolysis of elemental sulfur (S0) coupled to S0-based denitrification and denitritation was investigated in batch bioassays by microbiological and modeling approaches. In the denitrification experiments, the highest obtained NO3--N removal rate was 20.9\u202fmg/l\u00b7d. In the experiments with the biomass enriched on NO2-, a NO2--N removal rate of 10.7\u202fmg/l\u00b7d was achieved even at a NO2--N concentration as high as 240\u202fmg/l. The Helicobacteraceae family was only observed in the biofilm attached onto the chemically-synthesized S0 particles with a relative abundance up to 37.1%, suggesting it was the hydrolytic biomass capable of S0 solubilization in the novel surface-based model. S0-driven denitrification was modeled as a two-step process in order to explicitly account for the sequential reduction of NO3- to NO2- and then to N2 by denitrifying bacteria.", "keywords": ["Surface-based hydrolysis", "Autotrophic Processes", "Autotrophic denitrification; Autotrophic denitritation; Community structure; Elemental sulfur; Mathematical modeling; Surface-based hydrolysis", "Elemental sulfur", "Nitrates", "Nitrogen", "Hydrolysis", "0211 other engineering and technologies", "02 engineering and technology", "Autotrophic denitrification", "01 natural sciences", "6. Clean water", "Community structure", "Bioreactors", "Autotrophic denitritation", "Denitrification", "Autotrophic denitrification; Autotrophic denitritation; Elemental sulfur; Community structure; Surface-based hydrolysis; Mathematical modeling", "Mathematical modeling", "14. Life underwater", "Sulfur", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/698214/5/anastasiia%20JEMA.pdf"}, {"href": "https://doi.org/10.1016/j.jenvman.2018.01.064"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jenvman.2018.01.064", "name": "item", "description": "10.1016/j.jenvman.2018.01.064", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jenvman.2018.01.064"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-04-01T00:00:00Z"}}, {"id": "10.1016/j.nbt.2019.10.010", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:16:43Z", "type": "Journal Article", "created": "2019-11-12", "title": "Advances in combined enzymatic extraction of ferulic acid from wheat bran", "description": "Wheat bran could be utilised as feedstock for innovative and sustainable biorefinery processes. Here, an enzymatic hydrolysis process for ferulic acid (FA) extraction was optimised step by step for total wheat bran (Tritello) and then also applied to the outer bran layer (Bran 1). Proteins, reducing sugars, total phenols and FA were quantified. The highest FA yields (0.82-1.05\u202fg/kg bran) were obtained either by rehydrating the bran by autoclaving (Tritello) or by steam explosion (Bran 1) using a bran/water ratio of 1:20, followed by enzymatic pre-treatment with Alcalase and Termamyl, to remove protein and sugars, and a final enzymatic hydrolysis with Pentopan and feruloyl esterase to solubilise phenol. FA was recovered from the final digestate via solid phase extraction. A 40-fold scale-up was also performed and the release of compounds along all the process steps and at increasing incubation times was monitored. Results showed that FA was initially present at a minimum level while it was specifically released during the enzymatic treatment. In the final optimized process, the FA extraction yield was higher than that obtained with NaOH control hydrolysis while, in comparison with other FA enzymatic extraction methods, fewer process steps were required and no buffers, strong acid/alkali nor toxic compounds were used. Furthermore, the proposed process may be easily scaled-up, confirming the feasibility of wheat bran valorisation by biorefinery processes to obtain valuable compounds having several areas of potential industrial exploitation.", "keywords": ["Dietary Fiber", "0106 biological sciences", "0301 basic medicine", "Coumaric Acids", "Bioengineering", "Wheat bran", "01 natural sciences", "Bio-based Industries Innovation action - Demonstration", "Enzymatic extraction", "03 medical and health sciences", "Subtilisins", "European Commission", "Molecular Biology", "Knowmad Institut", "2. Zero hunger", "EC", "Hydrolysis", "H2020", "Polyphenols", "General Medicine", "Ferulic acid", "Biorefinery", "Biorefinery; Enzymatic extraction; Ferulic acid; Polyphenols; Steam explosion; Wheat bran", "Steam explosion", "alpha-Amylases", "Biotechnology"]}, "links": [{"href": "https://cris.unibo.it/bitstream/11585/706995/1/Ferri%20et%20al%202020%20New%20Biotech.pdf"}, {"href": "https://doi.org/10.1016/j.nbt.2019.10.010"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.nbt.2019.10.010", "name": "item", "description": "10.1016/j.nbt.2019.10.010", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.nbt.2019.10.010"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-01T00:00:00Z"}}, {"id": "10.1038/srep32791", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:17:55Z", "type": "Journal Article", "created": "2016-09-06", "title": "Responses Of Soil Hydrolytic Enzymes, Ammonia-Oxidizing Bacteria And Archaea To Nitrogen Applications In A Temperate Grassland In Inner Mongolia", "description": "Abstract<p>We used a seven-year urea gradient applied field experiment to investigate the effects of nitrogen (N) applications on soil N hydrolytic enzyme activity and ammonia-oxidizing microbial abundance in a typical steppe ecosystem in Inner Mongolia. The results showed that N additions inhibited the soil N-related hydrolytic enzyme activities, especially in 392\uffe2\uff80\uff89kg N ha\uffe2\uff88\uff921\uffe2\uff80\uff89yr\uffe2\uff88\uff921 treatment. As N additions increased, the amoA gene copy ratios of ammonia-oxidizing archaea (AOA) to ammonia-oxidizing bacteria (AOB) decreased from 1.13 to 0.65. Pearson correlation analysis showed that the AOA gene copies were negatively related with NH4+-N content. However, the AOB gene copies were positively correlated with NO3\uffe2\uff88\uff92-N content. Moderate N application rates (56\uffe2\uff80\uff93224\uffe2\uff80\uff89kg N ha\uffe2\uff88\uff921\uffe2\uff80\uff89yr\uffe2\uff88\uff921) accompanied by P additions are beneficial to maintaining the abundance of AOB, as opposed to the inhibition of highest N application rate (392\uffe2\uff80\uff89kg N ha\uffe2\uff88\uff921\uffe2\uff80\uff89yr\uffe2\uff88\uff921) on the abundance of AOB. This study suggests that the abundance of AOB and AOA would not decrease unless N applications exceed 224\uffe2\uff80\uff89kg N ha\uffe2\uff88\uff921\uffe2\uff80\uff89yr\uffe2\uff88\uff921 in temperate grasslands in Inner Mongolia.</p>", "keywords": ["2. Zero hunger", "China", "Bacteria", "Nitrogen", "Hydrolysis", "04 agricultural and veterinary sciences", "15. Life on land", "Archaea", "Grassland", "Nitrification", "Article", "Soil", "Ammonia", "0401 agriculture", " forestry", " and fisheries", "Fertilizers", "Oxidation-Reduction", "Ecosystem", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1038/srep32791"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/srep32791", "name": "item", "description": "10.1038/srep32791", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/srep32791"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-06T00:00:00Z"}}, {"id": "10.1111/1751-7915.14404", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:18:43Z", "type": "Journal Article", "created": "2024-04-08", "title": "Characterization of an extremophile bacterial acid phosphatase derived from metagenomics analysis", "description": "Abstract<p>Acid phosphatases are enzymes that play a crucial role in the hydrolysis of various organophosphorous molecules. A putative acid phosphatase called FS6 was identified using genetic profiles and sequences from different environments. FS6 showed high sequence similarity to type C acid phosphatases and retained more than 30% of consensus residues in its protein sequence. A histidine\uffe2\uff80\uff90tagged recombinant FS6 produced in Escherichia coli exhibited extremophile properties, functioning effectively in a broad pH range between 3.5 and 8.5. The enzyme demonstrated optimal activity at temperatures between 25 and 50\uffc2\uffb0C, with a melting temperature of 51.6\uffc2\uffb0C. Kinetic parameters were determined using various substrates, and the reaction catalysed by FS6 with physiological substrates was at least 100\uffe2\uff80\uff90fold more efficient than with p\uffe2\uff80\uff90nitrophenyl phosphate. Furthermore, FS6 was found to be a decamer in solution, unlike the dimeric forms of crystallized proteins in its family.</p", "keywords": ["0301 basic medicine", "Extremophiles", "0303 health sciences", "03 medical and health sciences", "Hydrolysis", "Acid Phosphatase", "Amino Acid Sequence", "Hydrogen-Ion Concentration", "TP248.13-248.65", "Research Articles", "Biotechnology", "Substrate Specificity"]}, "links": [{"href": "https://doi.org/10.1111/1751-7915.14404"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1751-7915.14404", "name": "item", "description": "10.1111/1751-7915.14404", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1751-7915.14404"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-04-01T00:00:00Z"}}, {"id": "10.2166/wst.2018.398", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:20:35Z", "type": "Journal Article", "created": "2018-10-04", "title": "Sensitivity analysis for an elemental sulfur-based two-step denitrification model", "description": "Abstract                <p>A local sensitivity analysis was performed for a chemically synthesized elemental sulfur (S0)-based two-step denitrification model, accounting for nitrite (NO2\uffe2\uff88\uff92) accumulation, biomass growth and S0 hydrolysis. The sensitivity analysis was aimed at verifying the model stability, understanding the model structure and individuating the model parameters to be further optimized. The mass specific area of the sulfur particles (a*) and hydrolysis kinetic constant (k1) were identified as the dominant parameters on the model outputs, i.e. nitrate (NO3\uffe2\uff88\uff92), NO2\uffe2\uff88\uff92 and sulfate (SO42\uffe2\uff88\uff92) concentrations, confirming that the microbially catalyzed S0 hydrolysis is the rate-limiting step during S0-driven denitrification. Additionally, the maximum growth rates of the denitrifying biomass on NO3\uffe2\uff88\uff92 and NO2\uffe2\uff88\uff92 were detected as the most sensitive kinetic parameters.</p>", "keywords": ["Elemental sulfur", "Environmental Engineering", "0207 environmental engineering", "Biological surface-based hydrolysis; Elemental sulfur; Mathematical modeling; Sensitivity analysis; Two-step autotrophic denitrification; Environmental Engineering; Water Science and Technology", "02 engineering and technology", "01 natural sciences", "Two-step autotrophic denitrification", "Bioreactors", "European Joint Doctorates", "European Commission", "Knowmad Institut", "Biological surface-based hydrolysis", "Nitrites", "Netherlands", "Water Science and Technology", "0105 earth and related environmental sciences", "Aurora Universities Network", "EC", "Nitrates", "H2020", "Energy Research", "13. Climate action", "Denitrification", "Mathematical modeling", "Sensitivity analysis", "Sulfur"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/724909/2/2018%20-%20Kostrytsia%20et%20al.%20-%20Water%20Science%20%26%20Technology%20-%20Sensitivity%20analysis%20for%20S0-based%20denitrification%20model.pdf"}, {"href": "http://iwaponline.com/wst/article-pdf/78/6/1296/504647/wst078061296.pdf"}, {"href": "https://doi.org/10.2166/wst.2018.398"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Water%20Science%20and%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2166/wst.2018.398", "name": "item", "description": "10.2166/wst.2018.398", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2166/wst.2018.398"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-09-20T00:00:00Z"}}, {"id": "10.3389/fmicb.2022.1032515", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:20:55Z", "type": "Journal Article", "created": "2022-11-09", "title": "The novel oligopeptide utilizing species Anaeropeptidivorans aminofermentans M3/9T, its role in anaerobic digestion and occurrence as deduced from large-scale fragment recruitment analyses", "description": "<p>Research on biogas-producing microbial communities aims at elucidation of correlations and dependencies between the anaerobic digestion (AD) process and the corresponding microbiome composition in order to optimize the performance of the process and the biogas output. Previously,Lachnospiraceaespecies were frequently detected in mesophilic to moderately thermophilic biogas reactors. To analyze adaptive genome features of a representativeLachnospiraceaestrain,Anaeropeptidivorans aminofermentansM3/9Twas isolated from a mesophilic laboratory-scale biogas plant and its genome was sequenced and analyzed in detail. Strain M3/9Tpossesses a number of genes encoding enzymes for degradation of proteins, oligo- and dipeptides. Moreover, genes encoding enzymes participating in fermentation of amino acids released from peptide hydrolysis were also identified. Based on further findings obtained from metabolic pathway reconstruction, M3/9Twas predicted to participate in acidogenesis within the AD process. To understand the genomic diversity between the biogas isolate M3/9Tand closely relatedAnaerotignumtype strains, genome sequence comparisons were performed. M3/9Tharbors 1,693 strain-specific genes among others encoding different peptidases, a phosphotransferase system (PTS) for sugar uptake, but also proteins involved in extracellular solute binding and import, sporulation and flagellar biosynthesis. In order to determine the occurrence of M3/9Tin other environments, large-scale fragment recruitments with the M3/9Tgenome as a template and publicly available metagenomes representing different environments was performed. The strain was detected in the intestine of mammals, being most abundant in goat feces, occasionally used as a substrate for biogas production.</p", "keywords": ["0301 basic medicine", "2. Zero hunger", "570 Biologie", "Microbiology", "7. Clean energy", "renewable energy", "QR1-502", "metagenome", "660.6", "03 medical and health sciences", "Lachnospiraceae", "ddc:570", "omics analyses", "plant biomass hydrolysis"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2022.1032515"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2022.1032515", "name": "item", "description": "10.3389/fmicb.2022.1032515", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2022.1032515"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-11-09T00:00:00Z"}}, {"id": "10.3390/pr9030491", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:15Z", "type": "Journal Article", "created": "2021-03-09", "title": "Sequential Hydrothermal Processing of Sewage Sludge to Produce Low Nitrogen Biocrude", "description": "<p>A hydrothermal pre-treatment has been developed to improve sewage sludge quality or to produce low nitrogen biocrude via hydrothermal liquefaction (HTL) in a subsequent step. The mild hydrothermal pre-treatment (150 \uffc2\uffb0C) step was performed with deionized water, sulfuric acid (0.5 M), or citric acid (0.5 M) to solubilize nitrogen containing compounds in the aqueous supernatant. Downstream, the residual solid material was liquefied with the addition of sodium carbonate via hydrothermal liquefaction (350 \uffc2\uffb0C). The pre-treatment with citric acid transferred up to 66.7 wt. % of nitrogen into the aqueous supernatant, while 62.0 wt. % of carbon was recovered in the solid. Due to the pre-treatment lipids retained in the sewage sludge solid, which increased the favored biocrude yield up to 42.9 wt. % and the quality evaluating value H/Ceff ratio significantly to 1.48. Multi-method characterization of the resulted biocrude samples showed a lower concentration of N-heterocycles, while long-chain aliphatics and free fatty acid are increased.</p>", "keywords": ["Technology", "ddc:600", "sewage sludge", "nitrogen extraction", "info:eu-repo/classification/ddc/600", "600", "sequential hydrothermal liquefaction", "hydrothermal hydrolysis", "pre-treatment", "6. Clean water", "12. Responsible consumption"]}, "links": [{"href": "http://www.mdpi.com/2227-9717/9/3/491/pdf"}, {"href": "https://www.mdpi.com/2227-9717/9/3/491/pdf"}, {"href": "https://doi.org/10.3390/pr9030491"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Processes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/pr9030491", "name": "item", "description": "10.3390/pr9030491", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/pr9030491"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-03-09T00:00:00Z"}}, {"id": "10481/92392", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:01Z", "type": "Journal Article", "created": "2024-04-08", "title": "Characterization of an extremophile bacterial acid phosphatase derived from metagenomics analysis", "description": "Abstract                   <p>                     Acid phosphatases are enzymes that play a crucial role in the hydrolysis of various organophosphorous molecules. A putative acid phosphatase called FS6 was identified using genetic profiles and sequences from different environments. FS6 showed high sequence similarity to type C acid phosphatases and retained more than 30% of consensus residues in its protein sequence. A histidine\uffe2\uff80\uff90tagged recombinant FS6 produced in                     Escherichia coli                     exhibited extremophile properties, functioning effectively in a broad pH range between 3.5 and 8.5. The enzyme demonstrated optimal activity at temperatures between 25 and 50\uffc2\uffb0C, with a melting temperature of 51.6\uffc2\uffb0C. Kinetic parameters were determined using various substrates, and the reaction catalysed by FS6 with physiological substrates was at least 100\uffe2\uff80\uff90fold more efficient than with                     p\uffe2\uff80\uff90                     nitrophenyl phosphate. Furthermore, FS6 was found to be a decamer in solution, unlike the dimeric forms of crystallized proteins in its family.                   </p", "keywords": ["0301 basic medicine", "Extremophiles", "0303 health sciences", "03 medical and health sciences", "Hydrolysis", "Acid Phosphatase", "Amino Acid Sequence", "Hydrogen-Ion Concentration", "TP248.13-248.65", "Research Articles", "Biotechnology", "Substrate Specificity"]}, "links": [{"href": "https://doi.org/10481/92392"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10481/92392", "name": "item", "description": "10481/92392", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10481/92392"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-04-01T00:00:00Z"}}, {"id": "20.500.11850/280881", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:42Z", "type": "Journal Article", "created": "2018-07-25", "title": "Biodegradation of synthetic polymers in soils: Tracking carbon into CO             2             and microbial biomass", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Stable isotope labeling of agricultural polyesters enables demonstration of their microbial utilization in soils.</p></article>", "keywords": ["0301 basic medicine", "Polymers", "Polyesters", "Spectrometry", " Mass", " Secondary Ion", "ENZYMATIC-HYDROLYSIS", "CATERPILLARS", "ECOLOGY", "7. Clean energy", "03 medical and health sciences", "Biomass", "SDG 2 \u2013 Kein Hunger", "SDG 2 - Zero Hunger", "POLYESTERS", "Research Articles", "Soil Microbiology", "2. Zero hunger", "106022 Mikrobiologie", "Carbon Isotopes", "0303 health sciences", "Fungi", "Agriculture", "Lipase", "Carbon Dioxide", "Carbon", "COPOLYESTERS", "Biodegradation", " Environmental", "13. 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Previously,Lachnospiraceaespecies were frequently detected in mesophilic to moderately thermophilic biogas reactors. To analyze adaptive genome features of a representativeLachnospiraceaestrain,Anaeropeptidivorans aminofermentansM3/9Twas isolated from a mesophilic laboratory-scale biogas plant and its genome was sequenced and analyzed in detail. Strain M3/9Tpossesses a number of genes encoding enzymes for degradation of proteins, oligo- and dipeptides. Moreover, genes encoding enzymes participating in fermentation of amino acids released from peptide hydrolysis were also identified. Based on further findings obtained from metabolic pathway reconstruction, M3/9Twas predicted to participate in acidogenesis within the AD process. To understand the genomic diversity between the biogas isolate M3/9Tand closely relatedAnaerotignumtype strains, genome sequence comparisons were performed. M3/9Tharbors 1,693 strain-specific genes among others encoding different peptidases, a phosphotransferase system (PTS) for sugar uptake, but also proteins involved in extracellular solute binding and import, sporulation and flagellar biosynthesis. In order to determine the occurrence of M3/9Tin other environments, large-scale fragment recruitments with the M3/9Tgenome as a template and publicly available metagenomes representing different environments was performed. The strain was detected in the intestine of mammals, being most abundant in goat feces, occasionally used as a substrate for biogas production.</p", "keywords": ["0301 basic medicine", "2. Zero hunger", "570 Biologie", "Microbiology", "7. 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