Antibiotics are some of the most widely used drugs. Their release in the environment is of great concern since their consumption is a major factor for antibiotic resistance, one of the most important threats to human health. Their occurrence and fate in agricultural systems have been extensively investigated in recent years. Yet whilst their biotic and abiotic degradation pathways have been thoroughly researched, their biotransformation pathways in plants are less understood, such as in case of trimethoprim. Although trimethoprim has been reported in the environment, its fate in higher plants still remains unknown. A bench-scale experiment was performed and 30 trimethoprim metabolites were identified in lettuce (Lactuca sativa L.), of which 5 belong to phase I and 25 to phase II. Data mining yielded a list of 1018 ions as possible metabolite candidates, which was filtered to a final list of 87 candidates. Molecular structures were assigned for 19 compounds, including 14 TMP metabolites reported for the first time. Alongside well-known biotransformation pathways in plants, additional novel pathways were suggested, namely, conjugation with sesquiterpene lactones, and abscisic acid as a part of phase II of plant metabolism. The results obtained offer insight into the variety of phase II conjugates and may serve as a guideline for studying the metabolization of other chemicals that share a similar molecular structure or functional groups with trimethoprim. Finally, the toxicity and potential contribution of the identified metabolites to the selective pressure on antibiotic resistance genes and bacterial communities via residual antimicrobial activity were evaluated.
", "keywords": ["2. Zero hunger", "High-resolution mass spectrometry", "Phytochemicals", "0211 other engineering and technologies", "02 engineering and technology", "01 natural sciences", "Trimethoprim", "Anti-Bacterial Agents", "3. Good health", "Conjugates", "Antibiotics", "Non-target screening", "Humans", "Plant metabolites", "Biotransformation", "Research Paper", "Lactuca", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Tadi\u0107, \u0110or\u0111e, Gramblicka, Michal, Mistrik, Robert, Bayona, Josep Maria,", "roles": ["creator"]}]}, "links": [{"href": "https://link.springer.com/content/pdf/10.1007/s00216-022-03943-6.pdf"}, {"href": "https://doi.org/10.1007/s00216-022-03943-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Analytical%20and%20Bioanalytical%20Chemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00216-022-03943-6", "name": "item", "description": "10.1007/s00216-022-03943-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00216-022-03943-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-09T00:00:00Z"}}, {"id": "10.1002/jsfa.7325", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:14:15Z", "type": "Journal Article", "created": "2015-06-30", "title": "Espresso coffee residues as a nitrogen amendment for small-scale vegetable production", "description": "Espresso coffee grounds constitute a residue which is produced daily in considerable amounts, and is often pointed out as being potentially interesting for plant nutrition. Two experiments (incubations and field experiments) were carried out to evaluate the potential nitrogen (N) and phosphorus (P) supply for carrot (Daucus carota L.), spinach (Spinacea oleracea L.) and lettuce (Lactuca sativa L.) nutrition.Immobilisation of nitrogen and phosphorus was detected in all the incubations and, in the field experiments, germination and yield growth were decreased by the presence of espresso coffee grounds, in general for all the species studied.The study showed an inhibition of N and P mineralisation and a reduction of plant germination and growth. Further research is required to determine whether this is related to the immobilising capacity of the residue or possibly due to the presence of caffeine.", "keywords": ["2. Zero hunger", "Minerals", "Nitrogen", "Plant Extracts", "0402 animal and dairy science", "Agriculture", "Coffea", "Germination", "Phosphorus", "04 agricultural and veterinary sciences", "Coffee", "01 natural sciences", "Daucus carota", "Soil", "Spinacia oleracea", "Caffeine", "Vegetables", "Humans", "0401 agriculture", " forestry", " and fisheries", "Biomass", "0405 other agricultural sciences", "Fertilizers", "Lactuca", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://onlinelibrary.wiley.com/wol1/doi/10.1002/jsfa.7325/fullpdf"}, {"href": "https://doi.org/10.1002/jsfa.7325"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20the%20Science%20of%20Food%20and%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/jsfa.7325", "name": "item", "description": "10.1002/jsfa.7325", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/jsfa.7325"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-07-28T00:00:00Z"}}, {"id": "10.1007/s11104-021-05133-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:09Z", "type": "Journal Article", "created": "2021-09-12", "title": "In situ laser manipulation of root tissues in transparent soil", "description": "AbstractAimsLaser micromanipulation such as dissection or optical trapping enables remote physical modification of the activity of tissues, cells and organelles. To date, applications of laser manipulation to plant roots grown in soil have been limited. Here, we show laser manipulation can be applied in situ when plant roots are grown in transparent soil.
MethodsWe have developed a Q-switched laser manipulation and imaging instrument to perform controlled dissection of roots and to study light-induced root growth responses. We performed a detailed characterisation of the properties of the cutting beams through the soil, studying dissection and optical ablation. Furthermore, we also studied the use of low light doses to control the root elongation rate of lettuce seedlings (Lactuca sativa) in air, agar, gel and transparent soil.
ResultsWe show that whilst soil inhomogeneities affect the thickness and circularity of the beam, those distortions are not inherently limiting. The ability to induce changes in root elongation or complete dissection of microscopic regions of the root is robust to substrate heterogeneity and microscopy set up and is maintained following the limited distortions induced by the transparent soil environment.
ConclusionsOur findings show that controlled in situ laser dissection of root tissues is possible with a simple and low-cost optical set-up. We also show that, in the absence of dissection, a reduced laser light power density can provide reversible control of root growth, achieving a precise \uffe2\uff80\uff9cpoint and shoot\uffe2\uff80\uff9d method for root manipulation.
", "keywords": ["Transparent soil", "/dk/atira/pure/subjectarea/asjc/1100/1111", "580", "0301 basic medicine", "570", "0303 health sciences", "Lactuca sativa", "name=Soil Science", "/dk/atira/pure/subjectarea/asjc/1100/1110", "Regular Article", "15. Life on land", "Imaging", "Laser dissection", "03 medical and health sciences", "Root", "name=Plant Science"]}, "links": [{"href": "https://link.springer.com/content/pdf/10.1007/s11104-021-05133-2.pdf"}, {"href": "https://doi.org/10.1007/s11104-021-05133-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20and%20Soil", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11104-021-05133-2", "name": "item", "description": "10.1007/s11104-021-05133-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11104-021-05133-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-09-12T00:00:00Z"}}, {"id": "10.1016/j.apsoil.2022.104678", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:45Z", "type": "Journal Article", "created": "2022-10-07", "title": "Trichoderma enriched compost, BCAs and potassium phosphite control Fusarium wilt of lettuce without affecting soil microbiome at genus level", "description": "Fusarium oxysporum f. sp. lactucae (Fol) is the causal agent of Fusarium wilt of lettuce, one of the most troublesome diseases affecting lettuce worldwide. Chemical control strategies are inadequate due to limited fungicide availability and consumer interest in organic vegetable production. Alternative control strategies, such as biological control agents (BCAs), suppressive compost, and resistance inducers, have been intensively studied to test their ability to reduce pathogen attacks. Research has been recently focused on the influence of BCAs on the rhizosphere microbiota, which plays a critical role in soil suppressiveness. In this work, three strategies of integrated pest management (IPM) were tested against Fol attacks in two fields for two consecutive years: (i) a compost enriched with Trichoderma, (ii) a combination of T. gamsii + T. asperellum, Bacillus amyloliquefaciens and potassium posphite and (iii) a combination of T. polysporum + T. atroviride. The rhizosphere microbiota was characterized by high-throughput sequencing of bacterial and eukaryotic rRNA gene markers. Obtained results indicated IPM strategies statistically reduced disease severity, in both fields and years, from 50 % to 70 % compared to untreated controls. An increased crop yield compared to untreated controls was also observed. Predominant phyla were Proteobacteria, Firmicutes and Actinobacteria for bacteria, and Ascomycota for fungi. However, microbiota populations were not affected by any of the treatments, nor were significant differences observed when the soil microbial community was compared to that of untreated controls. Conversely, large differences were observed when comparing the two fields and years, indicating an important microbial buffering effect triggered by the soil.", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Microbiota; Rhizosphere; Biocontrol agents; Resistance inducers; Seed born pathogen; Fusarium oxysporum f; sp; lactucae"], "contacts": [{"organization": "Bellini, A, Gilardi, G, Idbella, M, Zotti, M, Pugliese, M, Bonanomi, G, Gullino, ML,", "roles": ["creator"]}]}, "links": [{"href": "https://iris.unito.it/bitstream/2318/1891861/2/Bellini%20IRIS%20aperto.pdf"}, {"href": "https://iris.unito.it/bitstream/2318/1891861/8/Bellini%20post%20print_Pugliese.pdf"}, {"href": "https://doi.org/10.1016/j.apsoil.2022.104678"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Soil%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.apsoil.2022.104678", "name": "item", "description": "10.1016/j.apsoil.2022.104678", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.apsoil.2022.104678"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-02-01T00:00:00Z"}}, {"id": "10.1016/j.ecoenv.2012.01.003", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:58Z", "type": "Journal Article", "created": "2012-01-28", "title": "Effects Of Soil Dilution And Amendments (Mussel Shell, Cow Bone, And Biochar) On Pb Availability And Phytotoxicity In Military Shooting Range Soil", "description": "Bioavailability and bioaccessibility determine the level of metal toxicity in the soils. Inorganic soil amendments may decrease metal bioavailability and enhance soil quality. This study used mussel shell, cow bone, and biochar to reduce lead (Pb) toxicity in the highly contaminated military shooting range soil in Korea. Water-soluble and 1-M ammonium nitrate extractions, and a modified physiologically based extraction test (PBET) were performed to determine Pb bioavailability and bioaccessibility in the soil, respectively. Active C in the soil was also measured to evaluate the effects of the amendments on biological soil quality. The Pb contaminated soil was diluted in serial with uncontaminated soil for the bioassays. Seed germination and root elongation tests using lettuce (Lactuca sativa) showed increases in germination percentage and root length in soil treated with the amendments. Biochar was most effective and increased seed germination by 360% and root length by 189% compared to the unamended soil. Up to 20% soil dilution resulted in more than 50% seed germination. Bioavailability and bioaccessibility of Pb in the soils were decreased by 92.5% and 48.5% with mussel shell, by 84.8% and 34.5% with cow bone, and by 75.8% and 12.5% with biochar, respectively, compared to the unamended soil. We found that the Pb availability in the military shooting range soil can be reduced effectively by the tested amendments or soil dilution alternately, thereby decreasing the risk of ecotoxicity. Furthermore, the increasing active C from the amendments revitalized the soil contaminated with Pb.", "keywords": ["2. Zero hunger", "Korea", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "Bone and Bones", "6. Clean water", "Soil", "Lead", "Animal Shells", "Charcoal", "Animals", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Environmental Pollution", "Environmental Restoration and Remediation", "Lactuca", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.ecoenv.2012.01.003"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecotoxicology%20and%20Environmental%20Safety", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ecoenv.2012.01.003", "name": "item", "description": "10.1016/j.ecoenv.2012.01.003", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ecoenv.2012.01.003"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-05-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2020.114002", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:16:09Z", "type": "Journal Article", "created": "2020-01-21", "title": "Elucidating biotransformation pathways of ofloxacin in lettuce (Lactuca sativa L)", "description": "Antibiotics can be uptaken by plants from soil desorption or directly from irrigation water, but their metabolization pathways in plants are largely unknown. In this paper, an analytical workflow based on high-resolution mass spectrometry was applied for the systematic identification of biotransformation products of ofloxacin in lettuce. The targeted metabolites were selected by comparing the mass chromatograms of exposed with control samples using an advanced spectra-processing method (Fragment Ion Search). The innovative methodology presented allowed us to identify a total of 11 metabolites, including 5 ofloxacin metabolites that are being reported for the first time in plants. Accordingly, major transformation pathways were proposed revealing insight into how ofloxacin and related chemicals are metabolized in lettuce. Furthermore, the influence of biotransformation on potential residual antimicrobial activity of identified compounds was discussed. Human exposure to antibiotics at doses below the minimum inhibitory concentrations is crucial in human risk assessment, including food ingestion; however, in the case of ofloxacin presented results reveal that plant metabolites should also be considered so as not to underestimate their risk.", "keywords": ["High-resolution mass spectrometry", "Ofloxacin", "0211 other engineering and technologies", "02 engineering and technology", "Biotransformation pathways", "01 natural sciences", "6. Clean water", "Anti-Bacterial Agents", "Antibiotics", "Humans", "Plant metabolites", "Biotransformation", "Lactuca", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2020.114002"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2020.114002", "name": "item", "description": "10.1016/j.envpol.2020.114002", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2020.114002"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2012.12.014", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:16:08Z", "type": "Journal Article", "created": "2013-01-19", "title": "Reduced Bioaccumulation Of Pahs By Lactuca Satuva L. Grown In Contaminated Soil Amended With Sewage Sludge And Sewage Sludge Derived Biochar", "description": "The influence of sewage sludge (SS) and sewage sludge biochar (SSBC) upon biomass yield and the bioaccumulation of PAHs into lettuce plants grown in contaminated soil (\u221116PAH 20.2 \u00b1 0.9 mg kg(-1)) is presented. All SSBC amendments (2, 5 and 10%) and the 2% SS amendment significantly (P < 0.01) increased lettuce biomass. Both SS and SSBC amendments significantly reduced (P < 0.01) the bioaccumulation of PAHs at all application levels; with reduction in \u221116PAH concentration ranging between 41.8 and 60.3% in SS amended treatments and between 58.0 and 63.2% in SSBC amended treatments, with respect to the control. Benefits in terms of biomass production and PAHs bioaccumulation reduction were greatest where SSBC was used as a soil amendment. At high application rates (10%) SSBC reduced bioaccumulation of PAHs by between 56% and 67%, while SS reduced bioaccumulation of PAHs by less than 44%.", "keywords": ["Soil", "Sewage", "Charcoal", "Soil Pollutants", "Agriculture", "Polycyclic Aromatic Hydrocarbons", "Waste Disposal", " Fluid", "01 natural sciences", "Environmental Restoration and Remediation", "6. Clean water", "Lactuca", "0105 earth and related environmental sciences", "12. Responsible consumption"], "contacts": [{"organization": "Sardar Khan, Chao Cai, Ning Wang, Alessia Freddo, Alessia Freddo, Brian J. Reid,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2012.12.014"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2012.12.014", "name": "item", "description": "10.1016/j.envpol.2012.12.014", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2012.12.014"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-04-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2024.125307", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:16:10Z", "type": "Journal Article", "created": "2024-11-12", "title": "Biodegradable microplastics induce profound changes in lettuce (Lactuca sativa) defense mechanisms and to some extent deteriorate growth traits", "description": "The development of agricultural technologies has intensified the use of plastic in this sector. Products of plastic degradation, such as microplastics (MPs), potentially threaten living organisms, biodiversity and agricultural ecosystem functioning. Thus, biodegradable plastic materials have been introduced to agriculture. However, the effects of biodegradable plastic substitutes on soil ecosystems are even less known than those of traditional ones. Here, we studied the effects of environmentally relevant concentrations of MPs prepared from a biodegradable plastic (a starch-polybutylene adipate terephthalate blend, PBAT-BD-MPs) on the growth and defense mechanisms of lettuce (Lactuca sativa) in CLIMECS system (CLImatic Manipulation of ECosystem Samples). PBAT-BD-MPs in the highest concentrations negatively affected some traits of growth, i.e., dry weight percentage, specific leaf area, and both C and N contents. We observed more profound changes in plant physiology and biochemistry, as PBAT-BD-MPs decreased chlorophyll content and triggered a concerted response of plant defense mechanisms against oxidative stress. In conclusion, exposure to PBAT-BD-MPs induced plant oxidative stress and activated plant defense mechanisms, leading to oxidative homeostasis that sustained plant growth and functioning. Our study highlights the need for in-depth understanding of the effect of bioplastics on plants.", "keywords": ["580", "Chlorophyll", "0301 basic medicine", "570", "0303 health sciences", "salicylic acid", "Microplastics", "Lipid peroxidation", "lipid peroxidation", "Salicylic acid", "Biodegradable Plastics", "Plant Leaves", "Oxidative Stress", "03 medical and health sciences", "Starch-polybutylene adipate terephthalate", "Biodegradation", " Environmental", "total phenolic content", "starch-polybutylene adipate terephthalate", "Soil Pollutants", "PBAT", "Total phenolic content", "CLIMECS system", "Lactuca"], "contacts": [{"organization": "Sylwia Adamczyk, Laura J. Zantis, Sam van Loon, Cornelis A.M. van Gestel, Thijs Bosker, Rachel Hurley, Luca Nizzetto, Bartosz Adamczyk, Sannakajsa Velmala,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2024.125307"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2024.125307", "name": "item", "description": "10.1016/j.envpol.2024.125307", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2024.125307"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2023.122243", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:16:10Z", "type": "Journal Article", "created": "2023-07-21", "title": "Species-dependent responses of crop plants to polystyrene microplastics", "description": "Only recently there has been a strong focus on the impacts of microplastics on terrestrial crop plants. This study aims to examine and compare the effects of microplastics on two monocotyledonous (barley, Hordeum vulgare and wheat, Triticum aestivum), and two dicotyledonous (carrot, Daucus carota and lettuce, Lactuca sativa) plant species through two complimentary experiments. First, we investigated the effects of low, medium, and high (103, 105, 107 particles per mL) concentrations of 500\u00a0nm polystyrene microplastics (PS-MPs) on seed germination and early development. We found species-dependent effects on the early development, with microplastics only significantly affecting lettuce and carrot. When acutely exposed during germination, PS-MPs significantly delayed the germination of lettuce by 24%, as well as promoted the shoot growth of carrot by 71% and decreased its biomass by 26%. No effect was recorded on monocot species. Secondly, we performed a chronic (21\u00a0d) hydroponic experiment on lettuce and wheat. We observed that PS-MPs significantly reduced the shoot growth of lettuce by up to 35% and increased its biomass by up to 64%, while no record was reported on wheat. In addition, stress level indicators and defence mechanisms were significantly up-regulated in both lettuce and wheat seedlings. Overall, this study shows that PS-MPs affect plant development: impacts were recorded on both germination and growth for dicots, and responses identified by biochemical markers of stress were increased in both lettuce and wheat. This highlights species-dependent effects as the four crops were grown under identical conditions to allow direct comparison. For future research, our study emphasizes the need to focus on crop specific effects, while also working towards knowledge of plastic-induced impacts at environmentally relevant conditions.", "keywords": ["2. Zero hunger", "microplastics", "Microplastics", "Microplastic", "ta1183", "seed germination", "Biochemical indicators of stress", "Agriculture", "Germination", "plant growth", "15. Life on land", "Seed germination", "Seedlings", "Polystyrenes", "microplastic", "Plastics", "Triticum", "agriculture", "Plant growth", "Lactuca"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2023.122243"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2023.122243", "name": "item", "description": "10.1016/j.envpol.2023.122243", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2023.122243"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-01T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2007.07.014", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:16:39Z", "type": "Journal Article", "created": "2007-07-11", "title": "Accumulation Of Polycyclic Aromatic Hydrocarbons And Heavy Metals In Lettuce Grown In The Soils Contaminated With Long-Term Wastewater Irrigation", "description": "Accumulation of polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs) by crop plants from contaminated soils may pose health risks. A greenhouse pot experiment using lettuce (Lactuca satuva L.) as a representative vegetable was conducted to assess the concentrations of PAHs and HMs in vegetables grown in wastewater-contaminated soils. The concentrations of total PAHs were ranged from 1.5 to 3.4 mg kg(-1) in the contaminated soils, while 1.2 mg kg(-1) in the reference soil. Linear regression analyses showed that the relationships between soil and shoot PAH concentrations were stronger for LMW-PAHs (R(2) between 0.51 and 0.92) than for HMW-PAHs (R(2) 0.02 and 0.60), suggesting that translocation for LMW-PAHs is faster than HMW-PAHs. Furthermore, the data imply that root uptake was the main pathway for HMW-PAHs accumulation. The plant shoots were also highly contaminated with HMs, particularly Cd (0.4-0.9 mg kg(-1)), Cr (3.4-4.1 mg kg(-1)), Ni (11.7-15.1 mg kg(-1)) and Pb (2.3-5.3 mg kg(-1)), and exceed the guidance limits set by State Environmental Protection Administration (SEPA), China and the World Health Organization (WHO). This study highlights the potential health risks associated with cultivation and consumption of leafy vegetables on wastewater-contaminated soils.", "keywords": ["2. Zero hunger", "Agriculture", "Food Contamination", "15. Life on land", "01 natural sciences", "6. Clean water", "3. Good health", "Waste Management", "13. Climate action", "Metals", " Heavy", "11. Sustainability", "Soil Pollutants", "Polycyclic Aromatic Hydrocarbons", "Water Pollutants", " Chemical", "Lactuca", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.jhazmat.2007.07.014"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2007.07.014", "name": "item", "description": "10.1016/j.jhazmat.2007.07.014", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2007.07.014"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-04-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2024.176848", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:16:55Z", "type": "Journal Article", "created": "2024-10-10", "title": "Quantitative tracking of nanoplastics along the food chain from lettuce (Lactuca sativa) to snails (Cantareus aspersus)", "description": "Terrestrial systems are a significant sink for plastic contamination, including nano- and microplastics (NMPs). To date, limited information is available about the transfer of NMPs up the food web via trophic transfer, however, concerns about this exposure pathway for invertebrates and higher-level organisms have been raised. We aim to examine and quantify the trophic transfer of europium doped polystyrene nanoplastics (Eu-PS; NPs) within a terrestrial food chain. The uptake of 100\u00a0nm spherical Eu-PS particles from water through the roots of the plants to the leaves and finally to garden snails (Cantareus aspersus) was assessed. Lettuce (Lactuca sativa) was cultivated in Hoagland solution spiked with different concentrations of Eu-PS (15, 150 and 1500\u00a0\u03bcg/L) for three weeks. Then, lettuce shoots were used as food for snails for 19\u00a0days at a rate of 1\u00a0g of shoots per day. The Eu-PS primarily accumulated in the lettuce roots for all treatments, with a limited transfer to the shoots (only quantifiable in the highest treatment; translocation factor: TF\u00a0<\u00a01). No detectable levels of Eu-PS were found in the snails' digestive gland; however, the Eu-PS particles were detected in their feces (trophic transfer factor: TFF\u00a0>\u00a01). Moreover, only limited effects were observed on lettuce biomass by NPs treatments. No effects of the Eu-PS particles on snails were observed, with the exception of a consistent decrease in the shell diameter. Overall, our research illustrates that NPs can be absorbed by plants through their roots, subsequently transported to the shoots. However, our findings show limited transfer of NPs into snail tissues, but direct excretion into their feces. We provide an important insight into the potential transfer within the human food chain.", "keywords": ["Food Chain", "Human food basket", "Microplastics", "Snails", "Uptake", "Animals", "Invertebrate", "Plant", "Plastics transfer", "Water Pollutants", " Chemical", "Lactuca", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2024.176848"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2024.176848", "name": "item", "description": "10.1016/j.scitotenv.2024.176848", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2024.176848"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "10.1073/pnas.2109176118", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:18:11Z", "type": "Journal Article", "created": "2021-02-13", "title": "Plant-environment microscopy tracks interactions of Bacillus subtilis with plant roots across the entire rhizosphere", "description": "AbstractOur understanding of plant-microbe interactions in soil is limited by the difficulty of observing processes at the microscopic scale throughout plants\uffe2\uff80\uff99 large volume of influence. Here, we present the development of 3D live microscopy for resolving plant-microbe interactions across the environment of an entire seedling growing in a transparent soil in tailor-made mesocosms, maintaining physical conditions for the culture of both plants and microorganisms. A tailor made dual-illumination light-sheet system acquired scattering signals from the plant whilst fluorescence signals were captured from transparent soil particles and labelled microorganisms, allowing the generation of quantitative data on samples approximately 3600 mm3in size with as good as 5 \uffce\uffbcm resolution at a rate of up to one scan every 30 minutes. The system tracked the movement ofBacillus subtilispopulations in the rhizosphere of lettuce plants in real time, revealing previously unseen patterns of activity. Motile bacteria favoured small pore spaces over the surface of soil particles, colonising the root in a pulsatile manner. Migrations appeared to be directed towards the root cap, the point \uffe2\uff80\uff9cfirst contact\uffe2\uff80\uff9d, before subsequent colonisation of mature epidermis cells. Our findings show that microscopes dedicated to live environmental studies present an invaluable tool to understand plant-microbe interactions.
", "keywords": ["0301 basic medicine", "570", "Microscopy", "Silicon", "0303 health sciences", "Temperature", "root-microbe interactions", "Equipment Design", "Biological Sciences", "Environment", "15. Life on land", "Plant Roots", "630", "Fluorescence", "Soil", "03 medical and health sciences", "Seedlings", "Calibration", "Rhizosphere", "Image Processing", " Computer-Assisted", "environmental imaging", "rhizosphere", "Soil Microbiology", "Bacillus subtilis", "Lactuca"]}, "links": [{"href": "https://eprints.whiterose.ac.uk/178939/18/e2109176118.full.pdf"}, {"href": "https://pnas.org/doi/pdf/10.1073/pnas.2109176118"}, {"href": "https://doi.org/10.1073/pnas.2109176118"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Proceedings%20of%20the%20National%20Academy%20of%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1073/pnas.2109176118", "name": "item", "description": "10.1073/pnas.2109176118", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1073/pnas.2109176118"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-13T00:00:00Z"}}, {"id": "10.1093/jxb/erac437", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:18:29Z", "type": "Journal Article", "created": "2022-11-02", "title": "In situcontrol of root\u2013bacteria interactions using optical trapping in transparent soil", "description": "AbstractBacterial attachment on root surfaces is an important step preceding the colonization or internalization and subsequent infection of plants by pathogens. Unfortunately, bacterial attachment is not well understood because the phenomenon is difficult to observe. Here we assessed whether this limitation could be overcome using optical trapping approaches. We have developed a system based on counter-propagating beams and studied its ability to guide Pectobacterium atrosepticum (Pba) cells to different root cell types within the interstices of transparent soils. Bacterial cells were successfully trapped and guided to root hair cells, epidermal cells, border cells, and tissues damaged by laser ablation. Finally, we used the system to quantify the bacterial cell detachment rate of Pba cells on root surfaces following reversible attachment. Optical trapping techniques could greatly enhance our ability to deterministically characterize mechanisms linked to attachment and formation of biofilms in the rhizosphere.The rhizosphere hosts complex and abundant microbiomes whose structure and composition are now well described by metagenomic studies. However, the dynamic mechanisms that enable micro-organisms to establish along a growing plant root are poorly characterized. Here, we studied how a motile bacterium utilizes the microhabitats created by soil pore space to establish in the proximity of plant roots. We have established a model system consisting of Bacillus subtilis and lettuce seedlings co-inoculated in transparent soil microcosms. We carried out live imaging experiments and developed image analysis pipelines to quantify the abundance of the bacterium as a function of time and position in the pore space. Results showed that the establishment of the bacterium in the rhizosphere follows a precise sequence of events where small islands of mobile bacteria were first seen forming near the root tip within the first 12\uffe2\uff80\uff9324\uffe2\uff80\uff89h of inoculation. Biofilm was then seen forming on the root epidermis at distances of about 700\uffe2\uff80\uff931000\uffe2\uff80\uff89\uffc2\uffb5m from the tip. Bacteria accumulated predominantly in confined pore spaces within 200\uffe2\uff80\uff89\uffc2\uffb5m from the root or the surface of a particle. Using probabilistic models, we could map the complete sequence of events and propose a conceptual model of bacterial establishment in the pore space. This study therefore advances our understanding of the respective role of growth and mobility in the efficient colonization of bacteria in the rhizosphere.Our understanding of plant-microbe interactions in soil is limited by the difficulty of observing processes at the microscopic scale throughout plants\uffe2\uff80\uff99 large volume of influence. Here, we present the development of 3D live microscopy for resolving plant-microbe interactions across the environment of an entire seedling growing in a transparent soil in tailor-made mesocosms, maintaining physical conditions for the culture of both plants and microorganisms. A tailor made dual-illumination light-sheet system acquired scattering signals from the plant whilst fluorescence signals were captured from transparent soil particles and labelled microorganisms, allowing the generation of quantitative data on samples approximately 3600 mm3in size with as good as 5 \uffce\uffbcm resolution at a rate of up to one scan every 30 minutes. The system tracked the movement ofBacillus subtilispopulations in the rhizosphere of lettuce plants in real time, revealing previously unseen patterns of activity. Motile bacteria favoured small pore spaces over the surface of soil particles, colonising the root in a pulsatile manner. Migrations appeared to be directed towards the root cap, the point \uffe2\uff80\uff9cfirst contact\uffe2\uff80\uff9d, before subsequent colonisation of mature epidermis cells. Our findings show that microscopes dedicated to live environmental studies present an invaluable tool to understand plant-microbe interactions.Concerns over the possible increase in phytoavailability of biosolids\uffe2\uff80\uff90applied trace metals to plants have been raised based on the assumption that decomposition of applied organic matter would increase phytoavailability. The objectives of this study were to assess the effect of time on chemical extractability and concentration of Cd, Cu, Ni, and Zn in plants on plots established by a single application of biosolids with high trace metals content in 1984. Biosolids were applied to 1.5 by 2.3 m confined plots of a Davidson clay loam (clayey, kaolinitic, thermic Rhodic Kandiudults) at 0, 42, 84, 126, 168, and 210 Mg ha\uffe2\uff88\uff921 The highest biosolids application supplied 4.5, 760, 43, and 620 kg ha\uffe2\uff88\uff921 of Cd, Cu, Ni, and Zn, respectively. Radish (Raphanus sativus L.), romaine lettuce (Lactuca sativa L. varlongifolia), and barley (Hordeum vulgare L.) were planted at the site for 3 consecutive years, 17 to 19 yr after biosolids application. Extractable Cd, Cu, Ni, and Zn (as measured by DTPA, CaCl2, and Mehlich\uffe2\uff80\uff901) were determined on 15\uffe2\uff80\uff90cm depth samples from each plot. The DTPA\uffe2\uff80\uff90extractable Cu and Zn decreased by 58 and 42%, respectively, 17 yr after application despite a significant reduction in organic matter content. Biosolids treatments had no significant effect on crop yield. Plant tissue metal concentrations increased with biosolids rate but were within the normal range of these crops. Trace metal concentrations in plants generally correlated well with the concentrations extracted from soil with DTPA, CaCl2, and Mehlich\uffe2\uff80\uff901. Metal concentrations in plant tissue exhibited a plateau response in most cases. The uptake coefficient values generated for the different crops were in agreement with the values set by the Part 503 Rule.
", "keywords": ["2. Zero hunger", "Virginia", "Biological Availability", "Hordeum", "04 agricultural and veterinary sciences", "Pentetic Acid", "15. Life on land", "01 natural sciences", "6. Clean water", "Raphanus", "Refuse Disposal", "Trace Elements", "Calcium Chloride", "Soil", "Zinc", "Nickel", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Copper", "Cadmium", "Lactuca", "0105 earth and related environmental sciences"], "contacts": [{"organization": "L. W. Zelazny, Beshr Sukkariyah, Gregory K. Evanylo, Rufus L. Chaney,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.2134/jeq2004.0369"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Quality", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.2134/jeq2004.0369", "name": "item", "description": "10.2134/jeq2004.0369", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.2134/jeq2004.0369"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2005-11-01T00:00:00Z"}}, {"id": "10261/396978", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:24:55Z", "type": "Journal Article", "created": "2022-09-27", "title": "Risk assessment for uptake and accumulation of pharmaceuticals by baby leaf lettuce irrigated with reclaimed water under commercial agricultural activities", "description": "The use of reclaimed water to irrigate agricultural crops has increased in recent years as a consequence of water shortage constituting a potential risk for human health. The main objective of this study was to evaluate the impact on the soil-plant system and determining the accumulation of carbamazepine (CBZ), diclofenac (DCF), ketoprofen (KTP) and naproxen (NPX) in the edible part of lettuce under commonly used agricultural practices in commercial production. For this purpose, red oak baby lettuce (Lactuca sativa L.) was irrigated with reclaimed water fortified with different concentrations of pharmaceuticals. The study was carried out in two different scenarios: soil and tray. The tray experiments were conducted with substrate and took place at three different seasons of the year. Lettuce tissue sampled from these experiments were analysed 3 times during the lettuce growing cycle (first, second and third harvest). The practices of first harvest regrowth were also evaluated. For all experiments, CBZ showed the highest accumulation in lettuce leaves of the pharmaceuticals tested, showing a correlation between irrigation exposure time and pharmaceutical uptake. Unexpectedly, DCF was the compound with the highest uptake levels after regrowth practices. Results suggested that pharmaceuticals uptake could be directly associated with the irrigation method and possible accumulation in soil and substrates, while concentration of pharmaceuticals in substrates were 10 times higher. Based on the concentration values detected in lettuce leaves, the risk assessment suggests that no compounds imply any risk to human health, except CBZ for those on vegetarian diets in the tray scenario. Although commercial agricultural practices are usually not considered with regards to risk reduction, in this experiment we demonstrated that climatic conditions are a key factor in pharmaceuticals uptake and different agricultural practices (soil cropping and drip irrigation) can limit the presence of pharmaceutical compounds in crops.", "keywords": ["Crops", " Agricultural", "2. Zero hunger", "Agricultural Irrigation", "Diclofenac", "0211 other engineering and technologies", "Plant uptake emerging risk", "Water", "02 engineering and technology", "Wastewater", "Risk Assessment", "01 natural sciences", "6. Clean water", "Soil accumulation", "3. Good health", "Agricultural practices", "Soil", "Carbamazepine", "Pharmaceutical Preparations", "Regenerated water irrigation", "Humans", "Water Pollutants", " Chemical", "Lactuca", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10261/396978"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Environmental%20Management", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10261/396978", "name": "item", "description": "10261/396978", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10261/396978"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-01T00:00:00Z"}}, {"id": "10029/626877", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:24:40Z", "type": "Journal Article", "created": "2023-07-21", "title": "Species-dependent responses of crop plants to polystyrene microplastics", "description": "Only recently there has been a strong focus on the impacts of microplastics on terrestrial crop plants. This study aims to examine and compare the effects of microplastics on two monocotyledonous (barley, Hordeum vulgare and wheat, Triticum aestivum), and two dicotyledonous (carrot, Daucus carota and lettuce, Lactuca sativa) plant species through two complimentary experiments. First, we investigated the effects of low, medium, and high (103, 105, 107 particles per mL) concentrations of 500\u00a0nm polystyrene microplastics (PS-MPs) on seed germination and early development. We found species-dependent effects on the early development, with microplastics only significantly affecting lettuce and carrot. When acutely exposed during germination, PS-MPs significantly delayed the germination of lettuce by 24%, as well as promoted the shoot growth of carrot by 71% and decreased its biomass by 26%. No effect was recorded on monocot species. Secondly, we performed a chronic (21\u00a0d) hydroponic experiment on lettuce and wheat. We observed that PS-MPs significantly reduced the shoot growth of lettuce by up to 35% and increased its biomass by up to 64%, while no record was reported on wheat. In addition, stress level indicators and defence mechanisms were significantly up-regulated in both lettuce and wheat seedlings. Overall, this study shows that PS-MPs affect plant development: impacts were recorded on both germination and growth for dicots, and responses identified by biochemical markers of stress were increased in both lettuce and wheat. This highlights species-dependent effects as the four crops were grown under identical conditions to allow direct comparison. For future research, our study emphasizes the need to focus on crop specific effects, while also working towards knowledge of plastic-induced impacts at environmentally relevant conditions.", "keywords": ["2. Zero hunger", "Microplastics", "Microplastic", "ta1183", "Biochemical indicators of stress", "Agriculture", "Germination", "15. Life on land", "Seed germination", "Seedlings", "Polystyrenes", "microplastic", "Plastics", "Triticum", "agriculture", "Plant growth", "Lactuca"]}, "links": [{"href": "https://doi.org/10029/626877"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10029/626877", "name": "item", "description": "10029/626877", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10029/626877"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-01T00:00:00Z"}}, {"id": "1887/4097823", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:32Z", "type": "Journal Article", "created": "2024-10-10", "title": "Quantitative tracking of nanoplastics along the food chain from lettuce (Lactuca sativa) to snails (Cantareus aspersus)", "description": "Terrestrial systems are a significant sink for plastic contamination, including nano- and microplastics (NMPs). To date, limited information is available about the transfer of NMPs up the food web via trophic transfer, however, concerns about this exposure pathway for invertebrates and higher-level organisms have been raised. We aim to examine and quantify the trophic transfer of europium doped polystyrene nanoplastics (Eu-PS; NPs) within a terrestrial food chain. The uptake of 100\u00a0nm spherical Eu-PS particles from water through the roots of the plants to the leaves and finally to garden snails (Cantareus aspersus) was assessed. Lettuce (Lactuca sativa) was cultivated in Hoagland solution spiked with different concentrations of Eu-PS (15, 150 and 1500\u00a0\u03bcg/L) for three weeks. Then, lettuce shoots were used as food for snails for 19\u00a0days at a rate of 1\u00a0g of shoots per day. The Eu-PS primarily accumulated in the lettuce roots for all treatments, with a limited transfer to the shoots (only quantifiable in the highest treatment; translocation factor: TF\u00a0<\u00a01). No detectable levels of Eu-PS were found in the snails' digestive gland; however, the Eu-PS particles were detected in their feces (trophic transfer factor: TFF\u00a0>\u00a01). Moreover, only limited effects were observed on lettuce biomass by NPs treatments. No effects of the Eu-PS particles on snails were observed, with the exception of a consistent decrease in the shell diameter. Overall, our research illustrates that NPs can be absorbed by plants through their roots, subsequently transported to the shoots. However, our findings show limited transfer of NPs into snail tissues, but direct excretion into their feces. We provide an important insight into the potential transfer within the human food chain.", "keywords": ["Food Chain", "Human food basket", "Microplastics", "Snails", "Uptake", "Animals", "Invertebrate", "Plant", "Plastics transfer", "Water Pollutants", " Chemical", "Lactuca", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/1887/4097823"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "1887/4097823", "name": "item", "description": "1887/4097823", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/1887/4097823"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "1887/4172281", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:32Z", "type": "Journal Article", "created": "2024-11-12", "title": "Biodegradable microplastics induce profound changes in lettuce (Lactuca sativa) defense mechanisms and to some extent deteriorate growth traits", "description": "The development of agricultural technologies has intensified the use of plastic in this sector. Products of plastic degradation, such as microplastics (MPs), potentially threaten living organisms, biodiversity and agricultural ecosystem functioning. Thus, biodegradable plastic materials have been introduced to agriculture. However, the effects of biodegradable plastic substitutes on soil ecosystems are even less known than those of traditional ones. Here, we studied the effects of environmentally relevant concentrations of MPs prepared from a biodegradable plastic (a starch-polybutylene adipate terephthalate blend, PBAT-BD-MPs) on the growth and defense mechanisms of lettuce (Lactuca sativa) in CLIMECS system (CLImatic Manipulation of ECosystem Samples). PBAT-BD-MPs in the highest concentrations negatively affected some traits of growth, i.e., dry weight percentage, specific leaf area, and both C and N contents. We observed more profound changes in plant physiology and biochemistry, as PBAT-BD-MPs decreased chlorophyll content and triggered a concerted response of plant defense mechanisms against oxidative stress. In conclusion, exposure to PBAT-BD-MPs induced plant oxidative stress and activated plant defense mechanisms, leading to oxidative homeostasis that sustained plant growth and functioning. Our study highlights the need for in-depth understanding of the effect of bioplastics on plants.", "keywords": ["580", "Chlorophyll", "0301 basic medicine", "570", "0303 health sciences", "salicylic acid", "Microplastics", "Lipid peroxidation", "lipid peroxidation", "Salicylic acid", "Biodegradable Plastics", "Plant Leaves", "Oxidative Stress", "03 medical and health sciences", "Starch-polybutylene adipate terephthalate", "Biodegradation", " Environmental", "total phenolic content", "starch-polybutylene adipate terephthalate", "Soil Pollutants", "PBAT", "Total phenolic content", "CLIMECS system", "Lactuca"]}, "links": [{"href": "https://doi.org/1887/4172281"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "1887/4172281", "name": "item", "description": "1887/4172281", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/1887/4172281"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "2318/1891861", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:26:00Z", "type": "Journal Article", "created": "2022-10-07", "title": "Trichoderma enriched compost, BCAs and potassium phosphite control Fusarium wilt of lettuce without affecting soil microbiome at genus level", "description": "Fusarium oxysporum f. sp. lactucae (Fol) is the causal agent of Fusarium wilt of lettuce, one of the most troublesome diseases affecting lettuce worldwide. Chemical control strategies are inadequate due to limited fungicide availability and consumer interest in organic vegetable production. Alternative control strategies, such as biological control agents (BCAs), suppressive compost, and resistance inducers, have been intensively studied to test their ability to reduce pathogen attacks. Research has been recently focused on the influence of BCAs on the rhizosphere microbiota, which plays a critical role in soil suppressiveness. In this work, three strategies of integrated pest management (IPM) were tested against Fol attacks in two fields for two consecutive years: (i) a compost enriched with Trichoderma, (ii) a combination of T. gamsii + T. asperellum, Bacillus amyloliquefaciens and potassium posphite and (iii) a combination of T. polysporum + T. atroviride. The rhizosphere microbiota was characterized by high-throughput sequencing of bacterial and eukaryotic rRNA gene markers. Obtained results indicated IPM strategies statistically reduced disease severity, in both fields and years, from 50 % to 70 % compared to untreated controls. An increased crop yield compared to untreated controls was also observed. Predominant phyla were Proteobacteria, Firmicutes and Actinobacteria for bacteria, and Ascomycota for fungi. However, microbiota populations were not affected by any of the treatments, nor were significant differences observed when the soil microbial community was compared to that of untreated controls. Conversely, large differences were observed when comparing the two fields and years, indicating an important microbial buffering effect triggered by the soil.", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Microbiota; Rhizosphere; Biocontrol agents; Resistance inducers; Seed born pathogen; Fusarium oxysporum f; sp; lactucae"]}, "links": [{"href": "https://iris.unito.it/bitstream/2318/1891861/2/Bellini%20IRIS%20aperto.pdf"}, {"href": "https://iris.unito.it/bitstream/2318/1891861/8/Bellini%20post%20print_Pugliese.pdf"}, {"href": "https://doi.org/2318/1891861"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Applied%20Soil%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2318/1891861", "name": "item", "description": "2318/1891861", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2318/1891861"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-02-01T00:00:00Z"}}, {"id": "3130873339", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:26:31Z", "type": "Journal Article", "created": "2021-02-13", "title": "Plant-environment microscopy tracks interactions of Bacillus subtilis with plant roots across the entire rhizosphere", "description": "AbstractOur understanding of plant-microbe interactions in soil is limited by the difficulty of observing processes at the microscopic scale throughout plants\uffe2\uff80\uff99 large volume of influence. Here, we present the development of 3D live microscopy for resolving plant-microbe interactions across the environment of an entire seedling growing in a transparent soil in tailor-made mesocosms, maintaining physical conditions for the culture of both plants and microorganisms. A tailor made dual-illumination light-sheet system acquired scattering signals from the plant whilst fluorescence signals were captured from transparent soil particles and labelled microorganisms, allowing the generation of quantitative data on samples approximately 3600 mm3in size with as good as 5 \uffce\uffbcm resolution at a rate of up to one scan every 30 minutes. The system tracked the movement ofBacillus subtilispopulations in the rhizosphere of lettuce plants in real time, revealing previously unseen patterns of activity. Motile bacteria favoured small pore spaces over the surface of soil particles, colonising the root in a pulsatile manner. Migrations appeared to be directed towards the root cap, the point \uffe2\uff80\uff9cfirst contact\uffe2\uff80\uff9d, before subsequent colonisation of mature epidermis cells. Our findings show that microscopes dedicated to live environmental studies present an invaluable tool to understand plant-microbe interactions.Laser micromanipulation such as dissection or optical trapping enables remote physical modification of the activity of tissues, cells and organelles. To date, applications of laser manipulation to plant roots grown in soil have been limited. Here, we show laser manipulation can be applied in situ when plant roots are grown in transparent soil.
MethodsWe have developed a Q-switched laser manipulation and imaging instrument to perform controlled dissection of roots and to study light-induced root growth responses. We performed a detailed characterisation of the properties of the cutting beams through the soil, studying dissection and optical ablation. Furthermore, we also studied the use of low light doses to control the root elongation rate of lettuce seedlings (Lactuca sativa) in air, agar, gel and transparent soil.
ResultsWe show that whilst soil inhomogeneities affect the thickness and circularity of the beam, those distortions are not inherently limiting. The ability to induce changes in root elongation or complete dissection of microscopic regions of the root is robust to substrate heterogeneity and microscopy set up and is maintained following the limited distortions induced by the transparent soil environment.
ConclusionsOur findings show that controlled in situ laser dissection of root tissues is possible with a simple and low-cost optical set-up. We also show that, in the absence of dissection, a reduced laser light power density can provide reversible control of root growth, achieving a precise \uffe2\uff80\uff9cpoint and shoot\uffe2\uff80\uff9d method for root manipulation.Bacterial attachment on root surfaces is an important step preceding the colonization or internalization and subsequent infection of plants by pathogens. Unfortunately, bacterial attachment is not well understood because the phenomenon is difficult to observe. Here we assessed whether this limitation could be overcome using optical trapping approaches. We have developed a system based on counter-propagating beams and studied its ability to guide Pectobacterium atrosepticum (Pba) cells to different root cell types within the interstices of transparent soils. Bacterial cells were successfully trapped and guided to root hair cells, epidermal cells, border cells, and tissues damaged by laser ablation. Finally, we used the system to quantify the bacterial cell detachment rate of Pba cells on root surfaces following reversible attachment. Optical trapping techniques could greatly enhance our ability to deterministically characterize mechanisms linked to attachment and formation of biofilms in the rhizosphere.The rhizosphere hosts complex and abundant microbiomes whose structure and composition are now well described by metagenomic studies. However, the dynamic mechanisms that enable micro-organisms to establish along a growing plant root are poorly characterized. Here, we studied how a motile bacterium utilizes the microhabitats created by soil pore space to establish in the proximity of plant roots. We have established a model system consisting of Bacillus subtilis and lettuce seedlings co-inoculated in transparent soil microcosms. We carried out live imaging experiments and developed image analysis pipelines to quantify the abundance of the bacterium as a function of time and position in the pore space. Results showed that the establishment of the bacterium in the rhizosphere follows a precise sequence of events where small islands of mobile bacteria were first seen forming near the root tip within the first 12\uffe2\uff80\uff9324\uffe2\uff80\uff89h of inoculation. Biofilm was then seen forming on the root epidermis at distances of about 700\uffe2\uff80\uff931000\uffe2\uff80\uff89\uffc2\uffb5m from the tip. Bacteria accumulated predominantly in confined pore spaces within 200\uffe2\uff80\uff89\uffc2\uffb5m from the root or the surface of a particle. Using probabilistic models, we could map the complete sequence of events and propose a conceptual model of bacterial establishment in the pore space. This study therefore advances our understanding of the respective role of growth and mobility in the efficient colonization of bacteria in the rhizosphere.Our understanding of plant-microbe interactions in soil is limited by the difficulty of observing processes at the microscopic scale throughout plants\uffe2\uff80\uff99 large volume of influence. Here, we present the development of 3D live microscopy for resolving plant-microbe interactions across the environment of an entire seedling growing in a transparent soil in tailor-made mesocosms, maintaining physical conditions for the culture of both plants and microorganisms. A tailor made dual-illumination light-sheet system acquired scattering signals from the plant whilst fluorescence signals were captured from transparent soil particles and labelled microorganisms, allowing the generation of quantitative data on samples approximately 3600 mm3in size with as good as 5 \uffce\uffbcm resolution at a rate of up to one scan every 30 minutes. The system tracked the movement ofBacillus subtilispopulations in the rhizosphere of lettuce plants in real time, revealing previously unseen patterns of activity. Motile bacteria favoured small pore spaces over the surface of soil particles, colonising the root in a pulsatile manner. Migrations appeared to be directed towards the root cap, the point \uffe2\uff80\uff9cfirst contact\uffe2\uff80\uff9d, before subsequent colonisation of mature epidermis cells. Our findings show that microscopes dedicated to live environmental studies present an invaluable tool to understand plant-microbe interactions.Laser micromanipulation such as dissection or optical trapping enables remote physical modification of the activity of tissues, cells and organelles. To date, applications of laser manipulation to plant roots grown in soil have been limited. Here, we show laser manipulation can be applied in situ when plant roots are grown in transparent soil.
MethodsWe have developed a Q-switched laser manipulation and imaging instrument to perform controlled dissection of roots and to study light-induced root growth responses. We performed a detailed characterisation of the properties of the cutting beams through the soil, studying dissection and optical ablation. Furthermore, we also studied the use of low light doses to control the root elongation rate of lettuce seedlings (Lactuca sativa) in air, agar, gel and transparent soil.
ResultsWe show that whilst soil inhomogeneities affect the thickness and circularity of the beam, those distortions are not inherently limiting. The ability to induce changes in root elongation or complete dissection of microscopic regions of the root is robust to substrate heterogeneity and microscopy set up and is maintained following the limited distortions induced by the transparent soil environment.
ConclusionsOur findings show that controlled in situ laser dissection of root tissues is possible with a simple and low-cost optical set-up. We also show that, in the absence of dissection, a reduced laser light power density can provide reversible control of root growth, achieving a precise \uffe2\uff80\uff9cpoint and shoot\uffe2\uff80\uff9d method for root manipulation.