{"type": "FeatureCollection", "features": [{"id": "10.1007/s00248-005-5156-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:14:40Z", "type": "Journal Article", "created": "2006-01-02", "title": "Microbial Responses To Long-Term N Deposition In A Semiarid Grassland", "description": "Nitrogen (N) enrichment of the biosphere is an expanding problem to which arid ecosystems may be particularly sensitive. In semiarid grasslands, scarce precipitation uncouples plant and microbial activities, and creates within the soil a spatial mosaic of rhizosphere and cyanobacterial crust communities. We investigated the impact of elevated N deposition on these soil microbial communities at a grama-dominated study site located incentral New Mexico (USA). The study plots were established in 1995 and receive 10 kg ha(-1) year(-1) of supplemental N in the form of NH(4)NO(3). Soil samples were collected in July 2004, following 2 years of severe drought, and again in March 2005 following a winter of record high precipitation. Soils were assayed for potential activities of 20 extracellular enzymes and N(2)O production. The rhizosphere and crust-associated soils had peptidase and peroxidase potentials that were extreme in relation to those of temperate soils. N addition enhanced glycosidase and phosphatase activities and depressed peptidase. In contrast to temperate forest soils, oxidative enzyme activity did not respond to N treatment. Across sampling dates, extracellular enzyme activity responses correlated with inorganic N concentrations. N(2)O generation did not vary significantly with soil cover or N treatment. Microbial responses to N deposition in this semiarid grassland were distinct from those of forest ecosystems and appear to be modulated by inorganic N accumulation, which is linked to precipitation patterns.", "keywords": ["2. Zero hunger", "Principal Component Analysis", "Ecology", "Nitrogen", "Climate", "beta-Glucosidase", "Nitrous Oxide", "04 agricultural and veterinary sciences", "15. Life on land", "Poaceae", "01 natural sciences", "Carbon", "6. Clean water", "Enzymes", "Trees", "Leucyl Aminopeptidase", "Soil", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Soil Microbiology", "Peroxidase", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s00248-005-5156-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-005-5156-y", "name": "item", "description": "10.1007/s00248-005-5156-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-005-5156-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-01-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2012.12.023", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:17:10Z", "type": "Journal Article", "created": "2013-01-08", "title": "Warming And Increased Precipitation Have Differential Effects On Soil Extracellular Enzyme Activities In A Temperate Grassland", "description": "Few studies have conducted the responses of soil extracellular enzyme activities (EEA) to climate change, especially over the long term. In this study, we investigated the six-year responses of soil EEA to warming and increased precipitation in a temperate grassland of northern China at two depths of 0-10 and 10-20 cm. These extracellular enzymes included carbon-acquisition enzymes (\u03b2-glucosidase, BG), nitrogen-acquisition enzymes (N-acetylglucosaminidase, NAG; Leucine aminopeptidase, LAP) and phosphorus-acquisition enzymes (acid and alkaline phosphatases). The results showed that warming significantly increased acid phosphatase at the 0-10 cm depth and NAG at the 10-20 cm depth, but dramatically decreased BG and acid phosphatase in the subsurface. In contrast, increased precipitation significantly increased NAG, LAP and alkaline phosphatase in the surface and NAG, LAP and acid phosphatase in the subsurface. There was a significant warming and increased precipitation interaction on BG in the subsurface. Redundancy analysis indicated that the patterns of EEA were mainly driven by soil pH and NH(4)(+)-N and NO(3)(-)-N in the surface, while by NH(4)(+)-N and microbial biomass in the subsurface. Our results suggested that soil EEA responded differentially to warming and increased precipitation at two depths in this region, which may have implications for carbon and nutrient cycling under climate change.", "keywords": ["570", "China", "Climate", "Climate Change", "Acid Phosphatase", "04 agricultural and veterinary sciences", "15. Life on land", "Poaceae", "Enzymes", "Leucyl Aminopeptidase", "Soil biology", "13. 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In mycology, there has long been the implicit assumption of a trade-off between growth and enzymatic investment, which we test here using a set of filamentous fungi from the same soil. For these fungi we measured growth rate (as colony radial extension) and enzymatic repertoire (activities of four enzymes: laccase, cellobiohydrolase, leucine aminopeptidase and acid phosphatase), and explored the interaction between the traits based on phylogenetically corrected methods. Our results support the existence of a trade-off, however only for the enzymes presumably representing a larger metabolic cost (laccase and cellobiohydrolase). Our study offers new insights into potential functional complementarity within the soil fungal community in ecosystem processes, and experimentally supports an enzymatic investment/growth rate trade-off underpinning phenomena including substrate succession.</p>", "keywords": ["0301 basic medicine", "2. 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