{"type": "FeatureCollection", "features": [{"id": "10.1007/s00425-017-2647-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:23Z", "type": "Journal Article", "created": "2017-01-04", "title": "The cost of surviving nitrogen excess: energy and protein demand in the lichen Cladonia portentosa as revealed by proteomic analysis", "description": "Different nitrogen forms affect different metabolic pathways in lichens. In particular, the most relevant changes in protein expression were observed in the fungal partner, with NO 3- mostly affecting the energetic metabolism and NH 4+ affecting transport and regulation of proteins and the energetic metabolism much more than NO 3- did. Excess deposition of reactive nitrogen is a well-known agent of stress for lichens, but which symbiont is most affected and how, remains a mystery. Using proteomics can expand our understanding of stress effects on lichens. We investigated the effects of different doses and forms of reactive nitrogen, with and without supplementary phosphorus and potassium, on the proteome of the lichen Cladonia portentosa growing in a 'real-world' simulation of nitrogen deposition. Protein expression changed with the nitrogen treatments but mostly in the fungal partner, with NO3- mainly affecting the energetic metabolism and NH4+ also affecting the protein synthesis machinery. The photobiont mainly responded overexpressing proteins involved in energy production. This suggests that in response to nitrogen stress, the photobiont mainly supports the defensive mechanisms initiated by the mycobiont with an increased energy production. Such surplus energy is then used by the cell to maintain functionality in the presence of NO3-, while a futile cycle of protein production can be hypothesized to be induced by NH4+ excess. External supply of potassium and phosphorus influenced differently the responses of particular enzymes, likely reflecting the many processes in which potassium exerts a regulatory function.", "keywords": ["Chlorophyll", "Proteomics", "0301 basic medicine", "570", "mycobiont", "Lichens", "Nitrogen", "Cell Respiration", "Nitrate", "Mass Spectrometry", "Molecular mechanism", "03 medical and health sciences", "nitrate", "Ammonia", "Electrophoresis", " Gel", " Two-Dimensional", "Photosynthesis", "Ammonium", " Molecular mechanism", " Mycobiont", " Nitrate", " Photobiont", " Stress response", "Ammonium; Molecular mechanism; Mycobiont; Nitrate; Photobiont; Stress response; Genetics; Plant Science", "0303 health sciences", "Nitrates", "Stress response", "Chlorophyll A", "stress response", "Mycobiont", "ammonium", "Photobiont", "photobiont", "molecular mechanism", "Energy Metabolism", "Ammonium"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s00425-017-2647-2.pdf"}, {"href": "https://doi.org/10.1007/s00425-017-2647-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Planta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00425-017-2647-2", "name": "item", "description": "10.1007/s00425-017-2647-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00425-017-2647-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-04T00:00:00Z"}}, {"id": "10.1007/s11356-017-0378-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:14:56Z", "type": "Journal Article", "created": "2017-10-09", "title": "Intra- and inter-specific variations in chitin in lichens along a N-deposition gradient", "description": "The mechanisms of nitrogen (N) tolerance in lichens are not yet fully understood. Here, we investigated how the increase of chitin content is related with N excess at inter- and intra-specific levels, by using species with differing ecological N tolerances (the tolerant Xanthoria parietina and Parmotrema hypoleucinum and the sensitive Evernia prunastri and Usnea sp.) and thalli of X. parietina and P. hypoleucinum from sites with different availabilities of N of agricultural origin (livestock), as confirmed by lichen N content and \u03b415N. Nitrogen, chitin (N-containing compound), and ergosterol contents were measured in lichen thalli. Nitrogen and chitin contents were higher in tolerant species than those in sensitive ones (inter-specific level) and in thalli collected from the N-polluted site than in thalli from the clean site (intra-specific level). We suggest that chitin contributes to N stress tolerance in lichens, and that excess N can be partially stored as chitin (non-toxic form) in the cell walls of tolerant species.", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Ascomycota", "Biological Variation", " Population", "Lichens", "Nitrogen", "Ergosterol", "Chitin", "15. Life on land"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s11356-017-0378-3.pdf"}, {"href": "https://doi.org/10.1007/s11356-017-0378-3"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11356-017-0378-3", "name": "item", "description": "10.1007/s11356-017-0378-3", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11356-017-0378-3"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-10-09T00:00:00Z"}}, {"id": "10.1016/j.envpol.2017.06.102", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:15:50Z", "type": "Journal Article", "created": "2017-07-13", "title": "Using nitrogen concentration and isotopic composition in lichens to spatially assess the relative contribution of atmospheric nitrogen sources in complex landscapes", "description": "Reactive nitrogen (Nr) is an important driver of global change, causing alterations in ecosystem biodiversity and functionality. Environmental assessments require monitoring the emission and deposition of both the amount and types of Nr. This is especially important in heterogeneous landscapes, as different land-cover types emit particular forms of Nr to the atmosphere, which can impact ecosystems distinctively. Such assessments require high spatial resolution maps that also integrate temporal variations, and can only be feasibly achieved by using ecological indicators. Our aim was to rank land-cover types according to the amount and form of emitted atmospheric Nr in a complex landscape with multiple sources of N. To do so, we measured and mapped nitrogen concentration and isotopic composition in lichen thalli, which we then related to land-cover data. Results suggested that, at the landscape scale, intensive agriculture and urban areas were the most important sources of Nr to the atmosphere. Additionally, the ocean greatly influences Nr in land, by providing air with low Nr concentration and a unique isotopic composition. These results have important consequences for managing air pollution at the regional level, as they provide critical information for modeling Nr emission and deposition across regional as well as continental scales.", "keywords": ["2. Zero hunger", "Air Pollutants", "Lichens", "Nitrogen Isotopes", "Portugal", "Atmosphere", "Nitrogen", "Urbanization", "Geographic Mapping", "Agriculture", "15. Life on land", "01 natural sciences", "6. Clean water", "Reactive nitrogen", "13. Climate action", "Nitrogen Fixation", "11. Sustainability", "Industry", "Isoscapes", "14. Life underwater", "Polution - Eutrophication", "Ecosystem", "Environmental Monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2017.06.102"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2017.06.102", "name": "item", "description": "10.1016/j.envpol.2017.06.102", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2017.06.102"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-11-01T00:00:00Z"}}, {"id": "10.1016/j.funbio.2019.08.008", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:16:06Z", "type": "Journal Article", "created": "2019-09-03", "title": "Unveiling annual growth chronologies from inter-nodal branch elongations in a fruticose lichen in southern Europe", "description": "Techniques for retrospective analysis of size dynamics at annual resolution remain poorly developed in lichens in general, and fruticose lichens in particular. Only a few attempts in very high latitudes suggested that growth might be studied as a chronosequence of inter-nodal branch elongations. Here we evaluated, for the first time, this hypothesis in a dry Mediterranean environment using the lichen Cladonia rangiformis as a case study. Mixed models supported a strong positive relationship between humidity measured as precipitation/PET and inter-nodal branch elongations. Importantly, model selection suggested that (i) the number of intermodal elongations were a major determinant of stem elongation, and (ii) a second-order temporal autocorrelation denoted legacies of environmental influences at least over the next 2\u00a0y. The strong growth-humidity relationship, along with the potential legacies observed, support the idea that inter-nodal branch elongations could be used to reconstruct growth chronologies at annual resolution in drylands. This finding highlights the high vulnerability of these organisms to rising aridity, and opens a new venue for climate reconstruction and other potential applications in Ecology and Earth Science disciplines.", "keywords": ["Europe", "0106 biological sciences", "Ascomycota", "Lichens", "Mediterranean Region", "13. Climate action", "Climate", "Humidity", "15. Life on land", "01 natural sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.funbio.2019.08.008"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Fungal%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.funbio.2019.08.008", "name": "item", "description": "10.1016/j.funbio.2019.08.008", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.funbio.2019.08.008"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-11-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2018.11.010", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:16:34Z", "type": "Journal Article", "created": "2018-11-03", "title": "\u03b415N of lichens reflects the isotopic signature of ammonia source", "description": "Although it is generally accepted that \u03b415N in lichen reflects predominating N isotope sources in the environment, confirmation of the direct correlation between lichen \u03b415N and atmospheric \u03b415N is still missing, especially under field conditions with most confounding factors controlled. To fill this gap and investigate the response of lichens with different tolerance to atmospheric N deposition, thalli of the sensitive Evernia prunastri and the tolerant Xanthoria parietina were exposed for ten weeks to different forms and doses of N in a field manipulation experiment where confounding factors were minimized. During this period, several parameters, namely total N, \u03b415N and chlorophyll a fluorescence, were measured. Under the experimental conditions, \u03b415N in lichens quantitatively responded to the \u03b415N of released gaseous ammonia (NH3). Although a high correlation between the isotopic signatures in lichen tissue and supplied N was found both in tolerant and sensitive species, chlorophyll a fluorescence indicated that the sensitive species very soon lost its photosynthetic functionality with increasing N availability. The most damaging response to the different N chemical forms was observed with dry deposition of NH3, although wet deposition of ammonium ions had a significant observable physiological impact. Conversely, there was no significant effect of nitrate ions on chlorophyll a fluorescence, implying differential sensitivity to dry deposition versus wet deposition and to ammonium versus nitrate in wet deposition. Evernia prunastri was most sensitive to NH3, then NH4+, with lowest sensitivity to NO3-. Moreover, these results confirm that lichen \u03b415N can be used to indicate the \u03b415N of atmospheric ammonia, providing a suitable tool for the interpretation of the spatial distribution of NH3 sources in relation to their \u03b415N signal.", "keywords": ["Air Pollutants", "Nitrates", "Lichens", "Nitrogen Isotopes", "Chlorophyll A", "0211 other engineering and technologies", "02 engineering and technology", "Models", " Theoretical", "chlorophyll a fluorescence", "01 natural sciences", "nitrogen deposition", "Xanthoria parietina", "Species Specificity", "Ammonia", "13. Climate action", "source spatial distribution", "biomonitoring", "physiological response", "Photosynthesis", "Environmental Monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2018.11.010"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2018.11.010", "name": "item", "description": "10.1016/j.scitotenv.2018.11.010", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2018.11.010"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-01T00:00:00Z"}}, {"id": "10.1111/gcb.14399", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:18:33Z", "type": "Journal Article", "created": "2018-07-14", "title": "Pathways regulating decreased soil respiration with warming in a biocrust\u2010dominated dryland", "description": "Abstract

A positive soil carbon (C)\uffe2\uff80\uff90climate feedback is embedded into the climatic models of the IPCC. However, recent global syntheses indicate that the temperature sensitivity of soil respiration (RS) in drylands, the largest biome on Earth, is actually lower in warmed than in control plots. Consequently, soil C losses with future warming are expected to be low compared with other biomes. Nevertheless, the empirical basis for these global extrapolations is still poor in drylands, due to the low number of field experiments testing the pathways behind the long\uffe2\uff80\uff90term responses of soil respiration (RS) to warming. Importantly, global drylands are covered with biocrusts (communities formed by bryophytes, lichens, cyanobacteria, fungi, and bacteria), and thus,RSresponses to warming may be driven by both autotrophic and heterotrophic pathways. Here, we evaluated the effects of 8\uffe2\uff80\uff90year experimental warming onRS, and the different pathways involved, in a biocrust\uffe2\uff80\uff90dominated dryland in southern Spain. We also assessed the overall impacts on soil organic C (SOC) accumulation over time. Across the years and biocrust cover levels, warming reducedRSby 0.30\uffc2\uffa0\uffce\uffbcmol\uffc2\uffa0CO2\uffc2\uffa0m\uffe2\uff88\uff922\uffc2\uffa0s\uffe2\uff88\uff921(95% CI\uffc2\uffa0=\uffc2\uffa0\uffe2\uff88\uff920.24 to 0.84), although the negative warming effects were only significant after 3\uffc2\uffa0years of elevated temperatures in areas with low initial biocrust cover. We found support for different pathways regulating the warming\uffe2\uff80\uff90induced reduction inRSat areas with low (microbial thermal acclimation via reduced soil mass\uffe2\uff80\uff90specific respiration and \uffce\uffb2\uffe2\uff80\uff90glucosidase enzymatic activity) vs. high (microbial thermal acclimation jointly with a reduction in autotrophic respiration from decreased lichen cover) initial biocrust cover. Our 8\uffe2\uff80\uff90year experimental study shows a reduction in soil respiration with warming and highlights that biocrusts should be explicitly included in modeling efforts aimed to quantify the soil C\uffe2\uff80\uff93climate feedback in drylands.Nonvascular photoautotrophs (NVP), including bryophytes, lichens, terrestrial algae, and cyanobacteria, are increasingly recognized as being essential to ecosystem functioning in many regions of the world. Current research suggests that climate change may pose a substantial threat to NVP, but the extent to which this will affect the associated ecosystem functions and services is highly uncertain. Here, we propose a research agenda to address this urgent question, focusing on physiological and ecological processes that link NVP to ecosystem functions while also taking into account the substantial taxonomic diversity across multiple ecosystem types. Accordingly, we developed a new categorization scheme, based on microclimatic gradients, which simplifies the high physiological and morphological diversity of NVP and world\uffe2\uff80\uff90wide distribution with respect to several broad habitat types. We found that habitat\uffe2\uff80\uff90specific ecosystem functions of NVP will likely be substantially affected by climate change, and more quantitative process understanding is required on: (1) potential for acclimation; (2) response to elevated CO2; (3)\uffc2\uffa0role of the microbiome; and (4) feedback to (micro)climate. We suggest an integrative approach of innovative, multimethod laboratory and field experiments and ecophysiological modelling, for which sustained scientific collaboration on NVP research will be essential.

Climate change and atmospheric nitrogen (N) deposition on drylands are greatly threatening these especially vulnerable areas. Soil biocrust-forming lichens in drylands can provide early indicators of these disturbances and play a pivotal role, as they contribute to key ecosystem services. In this study, we explored the effects of different long-term water availability regimes simulating climate changes and their interaction with N addition on the physiological response of the soil lichen Cladonia rangiferina. Three sets of this lichen were subjected to control, reduced watering, and reduced watering and N addition (40 kg NH4NO3 ha\u22121 year\u22121) treatments for 16 months. Finally, all samples were subjected to daily hydration cycles with N-enriched water at two levels (40 and 80 kg NH4NO3 ha\u22121 year\u22121) for 23 days. We found that reduced watering significantly decreased the vitality of this lichen, whereas N addition unexpectedly helped lichens subjected to reduced watering to cope with stress produced by high temperatures. We also found that long-term exposure to N addition contributed to the acclimation to higher N availability. Overall, our data suggest that the interactions between reduced watering and increased N supply and temperature have an important potential to reduce the physiological performance of this soil lichen.

", "keywords": ["0301 basic medicine", "QH301-705.5", "Synergetic effects", "soil lichens; soil biocrust; global change; climate change; biomonitoring; synergetic effects; reduced watering; aridity; drylands; Mediterranean ecosystems", "01 natural sciences", "Article", "03 medical and health sciences", "Climate change", "Reduced watering", "Biology (General)", "Global change", "global change", "Aridity", "0105 earth and related environmental sciences", "0303 health sciences", "Drylands", "15. Life on land", "synergetic effects", "soil lichens", "6. Clean water", "climate change", "13. Climate action", "biomonitoring", "Biomonitoring", "Mediterranean ecosystems", "Soil lichens", "soil biocrust", "Soil biocrust"]}, "links": [{"href": "http://www.mdpi.com/2309-608X/8/4/333/pdf"}, {"href": "https://repositorio.ulisboa.pt/bitstream/10451/52117/1/jof-08-00333-v2.pdf"}, {"href": "https://www.mdpi.com/2309-608X/8/4/333/pdf"}, {"href": "https://doi.org/10451/52117"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Fungi", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10451/52117", "name": "item", "description": "10451/52117", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10451/52117"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-03-23T00:00:00Z"}}, {"id": "2971608869", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:25:55Z", "type": "Journal Article", "created": "2019-09-03", "title": "Unveiling annual growth chronologies from inter-nodal branch elongations in a fruticose lichen in southern Europe", "description": "Techniques for retrospective analysis of size dynamics at annual resolution remain poorly developed in lichens in general, and fruticose lichens in particular. Only a few attempts in very high latitudes suggested that growth might be studied as a chronosequence of inter-nodal branch elongations. Here we evaluated, for the first time, this hypothesis in a dry Mediterranean environment using the lichen Cladonia rangiformis as a case study. Mixed models supported a strong positive relationship between humidity measured as precipitation/PET and inter-nodal branch elongations. Importantly, model selection suggested that (i) the number of intermodal elongations were a major determinant of stem elongation, and (ii) a second-order temporal autocorrelation denoted legacies of environmental influences at least over the next 2\u00a0y. The strong growth-humidity relationship, along with the potential legacies observed, support the idea that inter-nodal branch elongations could be used to reconstruct growth chronologies at annual resolution in drylands. This finding highlights the high vulnerability of these organisms to rising aridity, and opens a new venue for climate reconstruction and other potential applications in Ecology and Earth Science disciplines.", "keywords": ["Europe", "0106 biological sciences", "Ascomycota", "Lichens", "Mediterranean Region", "13. Climate action", "Climate", "Humidity", "15. Life on land", "01 natural sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/2971608869"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Fungal%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2971608869", "name": "item", "description": "2971608869", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2971608869"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-11-01T00:00:00Z"}}, {"id": "10261/336363", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:24:38Z", "type": "Journal Article", "created": "2019-07-15", "title": "Biocrust science and global change", "description": "Open AccessPeer reviewed", "keywords": ["0106 biological sciences", "2. Zero hunger", "Take urgent action to combat climate change and its impacts", "Lichens", "Climate", "Climate Change", "Publications", "Bryophytes", "Biological soil crusts", "15. Life on land", "Cyanobacteria", "01 natural sciences", "Virtual", "Soil", "13. Climate action", "Global environmental change", "http://metadata.un.org/sdg/13", "Desert Climate", "Ecosystem", "Biocrusts", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10261/336363"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10261/336363", "name": "item", "description": "10261/336363", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10261/336363"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-07-15T00:00:00Z"}}, {"id": "10261/348950", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:24:39Z", "type": "Other", "title": "GYPWORLD Africa: Setting an agenda for gypsum ecosystem research in southern Africa", "description": "Open AccessPeer reviewed", "keywords": ["Namib Desert", "Soil", "Lichens", "Namaqualand", "Edaphic endemics", "Plants"], "contacts": [{"organization": "Siebert, Stefan J., Palacio, Sara, Luzuriaga, Arantzazu L., Maggs K\u00f6lling, Gillian, Marais, Eugene, Matesanz, Silvia, Prieto, Mar\u00eda, Pueyo, Yolanda, Rajakaruna, Nishanta, S\u00e1nchez, Ana M., Claassens, Sarina,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10261/348950"}, {"rel": "self", "type": "application/geo+json", "title": "10261/348950", "name": "item", "description": "10261/348950", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10261/348950"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "2960475767", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:25:55Z", "type": "Journal Article", "created": "2019-07-15", "title": "Biocrust science and global change", "description": "Open AccessPeer reviewed", "keywords": ["0106 biological sciences", "2. Zero hunger", "Take urgent action to combat climate change and its impacts", "Lichens", "Climate", "Climate Change", "Publications", "Bryophytes", "Biological soil crusts", "15. Life on land", "Cyanobacteria", "01 natural sciences", "Virtual", "Soil", "13. Climate action", "Global environmental change", "http://metadata.un.org/sdg/13", "Desert Climate", "Ecosystem", "Biocrusts", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/2960475767"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2960475767", "name": "item", "description": "2960475767", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2960475767"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-07-15T00:00:00Z"}}, {"id": "10451/59767", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:24:46Z", "type": "Journal Article", "created": "2018-11-03", "title": "\u03b415N of lichens reflects the isotopic signature of ammonia source", "description": "Although it is generally accepted that \u03b415N in lichen reflects predominating N isotope sources in the environment, confirmation of the direct correlation between lichen \u03b415N and atmospheric \u03b415N is still missing, especially under field conditions with most confounding factors controlled. To fill this gap and investigate the response of lichens with different tolerance to atmospheric N deposition, thalli of the sensitive Evernia prunastri and the tolerant Xanthoria parietina were exposed for ten weeks to different forms and doses of N in a field manipulation experiment where confounding factors were minimized. During this period, several parameters, namely total N, \u03b415N and chlorophyll a fluorescence, were measured. Under the experimental conditions, \u03b415N in lichens quantitatively responded to the \u03b415N of released gaseous ammonia (NH3). Although a high correlation between the isotopic signatures in lichen tissue and supplied N was found both in tolerant and sensitive species, chlorophyll a fluorescence indicated that the sensitive species very soon lost its photosynthetic functionality with increasing N availability. The most damaging response to the different N chemical forms was observed with dry deposition of NH3, although wet deposition of ammonium ions had a significant observable physiological impact. Conversely, there was no significant effect of nitrate ions on chlorophyll a fluorescence, implying differential sensitivity to dry deposition versus wet deposition and to ammonium versus nitrate in wet deposition. Evernia prunastri was most sensitive to NH3, then NH4+, with lowest sensitivity to NO3-. Moreover, these results confirm that lichen \u03b415N can be used to indicate the \u03b415N of atmospheric ammonia, providing a suitable tool for the interpretation of the spatial distribution of NH3 sources in relation to their \u03b415N signal.", "keywords": ["Air Pollutants", "Nitrates", "Lichens", "Nitrogen Isotopes", "Chlorophyll A", "0211 other engineering and technologies", "02 engineering and technology", "Models", " Theoretical", "chlorophyll a fluorescence", "01 natural sciences", "nitrogen deposition", "Xanthoria parietina", "Species Specificity", "Ammonia", "13. Climate action", "source spatial distribution", "biomonitoring", "physiological response", "Photosynthesis", "Environmental Monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://repositorio.ulisboa.pt/bitstream/10451/59767/1/1-s2.0-S0048969718343560-main.pdf"}, {"href": "https://doi.org/10451/59767"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10451/59767", "name": "item", "description": "10451/59767", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10451/59767"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-01T00:00:00Z"}}, {"id": "11585/582157", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:25:00Z", "type": "Journal Article", "created": "2017-01-04", "title": "The cost of surviving nitrogen excess: energy and protein demand in the lichen Cladonia portentosa as revealed by proteomic analysis", "description": "Different nitrogen forms affect different metabolic pathways in lichens. In particular, the most relevant changes in protein expression were observed in the fungal partner, with NO 3- mostly affecting the energetic metabolism and NH 4+ affecting transport and regulation of proteins and the energetic metabolism much more than NO 3- did. Excess deposition of reactive nitrogen is a well-known agent of stress for lichens, but which symbiont is most affected and how, remains a mystery. Using proteomics can expand our understanding of stress effects on lichens. We investigated the effects of different doses and forms of reactive nitrogen, with and without supplementary phosphorus and potassium, on the proteome of the lichen Cladonia portentosa growing in a 'real-world' simulation of nitrogen deposition. Protein expression changed with the nitrogen treatments but mostly in the fungal partner, with NO3- mainly affecting the energetic metabolism and NH4+ also affecting the protein synthesis machinery. The photobiont mainly responded overexpressing proteins involved in energy production. This suggests that in response to nitrogen stress, the photobiont mainly supports the defensive mechanisms initiated by the mycobiont with an increased energy production. Such surplus energy is then used by the cell to maintain functionality in the presence of NO3-, while a futile cycle of protein production can be hypothesized to be induced by NH4+ excess. External supply of potassium and phosphorus influenced differently the responses of particular enzymes, likely reflecting the many processes in which potassium exerts a regulatory function.", "keywords": ["Chlorophyll", "Proteomics", "0301 basic medicine", "570", "mycobiont", "Lichens", "Nitrogen", "Cell Respiration", "Nitrate", "Mass Spectrometry", "Molecular mechanism", "03 medical and health sciences", "nitrate", "Ammonia", "Electrophoresis", " Gel", " Two-Dimensional", "Photosynthesis", "Ammonium", " Molecular mechanism", " Mycobiont", " Nitrate", " Photobiont", " Stress response", "Ammonium; Molecular mechanism; Mycobiont; Nitrate; Photobiont; Stress response; Genetics; Plant Science", "0303 health sciences", "Nitrates", "Stress response", "Chlorophyll A", "stress response", "Mycobiont", "ammonium", "Photobiont", "photobiont", "molecular mechanism", "Energy Metabolism", "Ammonium"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s00425-017-2647-2.pdf"}, {"href": "https://doi.org/11585/582157"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Planta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11585/582157", "name": "item", "description": "11585/582157", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11585/582157"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-04T00:00:00Z"}}, {"id": "11585/917373", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-26T16:25:00Z", "type": "Journal Article", "created": "2022-11-22", "title": "A research agenda for nonvascular photoautotrophs under climate change", "description": "Summary

Nonvascular photoautotrophs (NVP), including bryophytes, lichens, terrestrial algae, and cyanobacteria, are increasingly recognized as being essential to ecosystem functioning in many regions of the world. Current research suggests that climate change may pose a substantial threat to NVP, but the extent to which this will affect the associated ecosystem functions and services is highly uncertain. Here, we propose a research agenda to address this urgent question, focusing on physiological and ecological processes that link NVP to ecosystem functions while also taking into account the substantial taxonomic diversity across multiple ecosystem types. Accordingly, we developed a new categorization scheme, based on microclimatic gradients, which simplifies the high physiological and morphological diversity of NVP and world\uffe2\uff80\uff90wide distribution with respect to several broad habitat types. We found that habitat\uffe2\uff80\uff90specific ecosystem functions of NVP will likely be substantially affected by climate change, and more quantitative process understanding is required on: (1) potential for acclimation; (2) response to elevated CO2; (3)\uffc2\uffa0role of the microbiome; and (4) feedback to (micro)climate. We suggest an integrative approach of innovative, multimethod laboratory and field experiments and ecophysiological modelling, for which sustained scientific collaboration on NVP research will be essential.A positive soil carbon (C)\uffe2\uff80\uff90climate feedback is embedded into the climatic models of the IPCC. However, recent global syntheses indicate that the temperature sensitivity of soil respiration (RS) in drylands, the largest biome on Earth, is actually lower in warmed than in control plots. Consequently, soil C losses with future warming are expected to be low compared with other biomes. Nevertheless, the empirical basis for these global extrapolations is still poor in drylands, due to the low number of field experiments testing the pathways behind the long\uffe2\uff80\uff90term responses of soil respiration (RS) to warming. Importantly, global drylands are covered with biocrusts (communities formed by bryophytes, lichens, cyanobacteria, fungi, and bacteria), and thus,RSresponses to warming may be driven by both autotrophic and heterotrophic pathways. Here, we evaluated the effects of 8\uffe2\uff80\uff90year experimental warming onRS, and the different pathways involved, in a biocrust\uffe2\uff80\uff90dominated dryland in southern Spain. We also assessed the overall impacts on soil organic C (SOC) accumulation over time. Across the years and biocrust cover levels, warming reducedRSby 0.30\uffc2\uffa0\uffce\uffbcmol\uffc2\uffa0CO2\uffc2\uffa0m\uffe2\uff88\uff922\uffc2\uffa0s\uffe2\uff88\uff921(95% CI\uffc2\uffa0=\uffc2\uffa0\uffe2\uff88\uff920.24 to 0.84), although the negative warming effects were only significant after 3\uffc2\uffa0years of elevated temperatures in areas with low initial biocrust cover. We found support for different pathways regulating the warming\uffe2\uff80\uff90induced reduction inRSat areas with low (microbial thermal acclimation via reduced soil mass\uffe2\uff80\uff90specific respiration and \uffce\uffb2\uffe2\uff80\uff90glucosidase enzymatic activity) vs. high (microbial thermal acclimation jointly with a reduction in autotrophic respiration from decreased lichen cover) initial biocrust cover. Our 8\uffe2\uff80\uff90year experimental study shows a reduction in soil respiration with warming and highlights that biocrusts should be explicitly included in modeling efforts aimed to quantify the soil C\uffe2\uff80\uff93climate feedback in drylands.