{"type": "FeatureCollection", "features": [{"id": "10.1007/s11027-020-09916-3", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-25T16:16:11Z", "type": "Journal Article", "created": "2020-06-22", "title": "The effect of crop residues, cover crops, manures and nitrogen fertilization on soil organic carbon changes in agroecosystems: a synthesis of reviews", "description": "Abstract

International initiatives are emphasizing the capture of atmospheric CO2 in soil organic C (SOC) to reduce the climatic footprint from agroecosystems. One approach to quantify the contribution of management practices towards that goal is through analysis of long-term experiments (LTEs). Our objectives were to analyze knowledge gained in literature reviews on SOC changes in LTEs, to evaluate the results regarding interactions with pedo-climatological factors, and to discuss disparities among reviews in data selection criteria. We summarized mean response ratios (RRs) and stock change rate (SCR) effect size indices from twenty reviews using paired comparisons (N). The highest RRs were found with manure applications (30%, N\uffe2\uff80\uff89=\uffe2\uff80\uff89418), followed by aboveground crop residue retention and the use of cover crops (9\uffe2\uff80\uff9310%, N\uffe2\uff80\uff89=\uffe2\uff80\uff89995 and 129), while the effect of nitrogen fertilization was lowest (6%, N\uffe2\uff80\uff89=\uffe2\uff80\uff89846). SCR for nitrogen fertilization exceeded that for aboveground crop residue retention (233 versus 117\uffc2\uffa0kg\uffc2\uffa0C\uffc2\uffa0ha\uffe2\uff88\uff921\uffc2\uffa0year\uffe2\uff88\uff921, N\uffe2\uff80\uff89=\uffe2\uff80\uff89183 and 279) and was highest for manure applications and cover crops (409 and 331\uffc2\uffa0kg\uffc2\uffa0C\uffc2\uffa0ha\uffe2\uff88\uff921\uffc2\uffa0year\uffe2\uff88\uff921, N\uffe2\uff80\uff89=\uffe2\uff80\uff89217 and 176). When data allows, we recommend calculating both RR and SCR because it improves the interpretation. Our synthesis shows that results are not always consistent among reviews and that interaction with texture and climate remain inconclusive. Selection criteria for study durations are highly variable, resulting in irregular conclusions for the effect of time on changes in SOC. We also discuss the relationships of SOC changes with yield and cropping systems, as well as conceptual problems when scaling-up results obtained from field studies to regional levels.Unprecedented nitrogen (N) inputs into terrestrial ecosystems have profoundly altered soil N cycling. Ammonia oxidizers and denitrifiers are the main producers of nitrous oxide (N2O), but it remains unclear how ammonia oxidizer and denitrifier abundances will respond to N loading and whether their responses can predict N\uffe2\uff80\uff90induced changes in soil N2O emission. By synthesizing 101 field studies worldwide, we showed that N loading significantly increased ammonia oxidizer abundance by 107% and denitrifier abundance by 45%. The increases in both ammonia oxidizer and denitrifier abundances were primarily explained by N loading form, and more specifically, organic N loading had stronger effects on their abundances than mineral N loading. Nitrogen loading increased soil N2O emission by 261%, whereas there was no clear relationship between changes in soil N2O emission and shifts in ammonia oxidizer and denitrifier abundances. Our field\uffe2\uff80\uff90based results challenge the laboratory\uffe2\uff80\uff90based hypothesis that increased ammonia oxidizer and denitrifier abundances by N loading would directly cause higher soil N2O emission. Instead, key abiotic factors (mean annual precipitation, soil pH, soil C:N ratio, and ecosystem type) explained N\uffe2\uff80\uff90induced changes in soil N2O emission. Altogether, these findings highlight the need for considering the roles of key abiotic factors in regulating soil N transformations under N loading to better understand the microbially mediated soil N2O emission. Rising levels of atmospheric CO 2 are thought to increase C sinks in terrestrial ecosystems. The potential of these sinks to mitigate CO 2 emissions, however, may be constrained by nutrients. By using metaanalysis, we found that elevated CO 2 only causes accumulation of soil C when N is added at rates well above typical atmospheric N inputs. Similarly, elevated CO 2 only enhances N 2 fixation, the major natural process providing soil N input, when other nutrients (e.g., phosphorus, molybdenum, and potassium) are added. Hence, soil C sequestration under elevated CO 2 is constrained both directly by N availability and indirectly by nutrients needed to support N 2 fixation.

", "keywords": ["Greenhouse Effect", "Nitrogen", "cycles", "fine roots", "Plant Development", "01 natural sciences", "forest", "Soil", "Nitrogen Fixation", "elevated atmospheric co2", "Ecosystem", "0105 earth and related environmental sciences", "model", "biological nitrogen-fixation", "04 agricultural and veterinary sciences", "Carbon Dioxide", "Plants", "15. Life on land", "Carbon", "6. Clean water", "13. Climate action", "climate-change", "0401 agriculture", " forestry", " and fisheries", "grassland", "ecosystem responses", "metaanalysis"]}, "links": [{"href": "https://doi.org/10.1073/pnas.0509038103"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Proceedings%20of%20the%20National%20Academy%20of%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1073/pnas.0509038103", "name": "item", "description": "10.1073/pnas.0509038103", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1073/pnas.0509038103"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-04-25T00:00:00Z"}}, {"id": "10.1111/ejss.13090", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-25T16:21:39Z", "type": "Journal Article", "created": "2021-01-22", "title": "Inconsistent effects of agricultural practices on soil fungal communities across 12 European long\u2010term experiments", "description": "Abstract

Cropping practices have a great potential to improve soil quality through changes in soil biota. Yet the effects of these soil\uffe2\uff80\uff90improving cropping systems on soil fungal communities are not well known. Here, we analysed soil fungal communities using standardized measurements in 12 long\uffe2\uff80\uff90term experiments and 20 agricultural treatments across Europe. We were interested in whether the same practices (i.e., tillage, fertilization, organic amendments and cover crops) applied across different sites have predictable and repeatable effects on soil fungal communities and guilds. The fungal communities were very variable across sites located in different soil types and climatic regions. The arbuscular mycorrhizal fungi (AMF) were the fungal guild with most unique species in individual sites, whereas plant pathogenic fungi were most shared between the sites. The fungal communities responded to the cropping practices differently in different sites and only fertilization showed a consistent effect on AMF and plant pathogenic fungi, whereas the responses to tillage, cover crops and organic amendments were site, soil and crop\uffe2\uff80\uff90species specific. We further show that the crop yield is negatively affected by cropping practices aimed at improving soil health. Yet, we show that these practices have the potential to change the fungal communities and that change in plant pathogenic fungi and in AMF is linked to the yield. We further link the soil fungal community and guilds to soil abiotic characteristics and reveal that especially Mn, K, Mg and pH affect the composition of fungi across sites. In summary, we show that fungal communities vary considerably between sites and that there are no clear directional responses in fungi or fungal guilds across sites to soil\uffe2\uff80\uff90improving cropping systems, but that the responses vary based on soil abiotic conditions, crop type and climatic conditions.

Highlights

Soil fungi were analysed using standardized measurements in 12 long\uffe2\uff80\uff90term experiments and 20 agricultural treatments

Fungal communities responded to the cropping practices differently at different sites

Only reduced fertilization showed a consistent effect on AMF and plant pathogenic fungi, whereas the responses to tillage, cover crops and organic amendments were site specific.

Fungal community structure varied significantly between sites, crops and climate conditions; therefore, more cross\uffe2\uff80\uff90site studies are needed in order to manage beneficial soil fungi in agricultural systems.

", "keywords": ["soil‐", "DIVERSITY", "0607 Plant Biology", "0703 Crop and Pasture Production", "Soil Science", "ARBUSCULAR MYCORRHIZAL FUNGI", "FERTILIZATION", "improving cropping systems", "soil fungi", "0503 Soil Sciences", "S Agriculture (General)", "CROPS", "METAANALYSIS", "TILLAGE", "2. Zero hunger", "Science & Technology", "long‐", "LAND-USE", "soil-improving cropping systems", "Agriculture", "Agronomy & Agriculture", "04 agricultural and veterinary sciences", "PERFORMANCE", "15. Life on land", "4106 Soil sciences", "long-term experiments", "organic amendments", "tillage", "term experiments", "POPULATIONS", "0401 agriculture", " forestry", " and fisheries", "BIODIVERSITY", "Life Sciences & Biomedicine"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/ejss.13090"}, {"href": "https://rau.repository.guildhe.ac.uk/id/eprint/16456/1/ejss.13090.pdf"}, {"href": "https://doi.org/10.1111/ejss.13090"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/European%20Journal%20of%20Soil%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/ejss.13090", "name": "item", "description": "10.1111/ejss.13090", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/ejss.13090"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-18T00:00:00Z"}}, {"id": "10.1111/gcb.13446", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:21:44Z", "type": "Journal Article", "created": "2016-09-03", "title": "The unseen invaders: introduced earthworms as drivers of change in plant communities in North American forests (a meta-analysis)", "description": "Abstract

Globally, biological invasions can have strong impacts on biodiversity as well as ecosystem functioning. While less conspicuous than introduced aboveground organisms, introduced belowground organisms may have similarly strong effects. Here, we synthesize for the first time the impacts of introduced earthworms on plant diversity and community composition in North American forests. We conducted a meta\uffe2\uff80\uff90analysis using a total of 645 observations to quantify mean effect sizes of associations between introduced earthworm communities and plant diversity, cover of plant functional groups, and cover of native and non\uffe2\uff80\uff90native plants. We found that plant diversity significantly declined with increasing richness of introduced earthworm ecological groups. While plant species richness or evenness did not change with earthworm invasion, our results indicate clear changes in plant community composition: cover of graminoids and non\uffe2\uff80\uff90native plant species significantly increased, and cover of native plant species (of all functional groups) tended to decrease, with increasing earthworm biomass. Overall, these findings support the hypothesis that introduced earthworms facilitate particular plant species adapted to the abiotic conditions of earthworm\uffe2\uff80\uff90invaded forests. Further, our study provides evidence that introduced earthworms are associated with declines in plant diversity in North American forests. Changing plant functional composition in these forests may have long\uffe2\uff80\uff90lasting effects on ecosystem functioning.

", "keywords": ["0106 biological sciences", "NONNATIVE EARTHWORMS", "ECOSYSTEM ENGINEER", "introduced earthworms", "biological invasions", "SEEDLING ESTABLISHMENT", "Forests", "01 natural sciences", "BIOLOGICAL INVASIONS", "GLOBAL METAANALYSIS", "HARDWOOD FORESTS", "Journal Article", "BIODIVERSITY CHANGE", "Animals", "ENDOGEIC EARTHWORMS", "earthworm invasion", "community composition", "Oligochaeta", "Ecosystem", "Biodiversity", "Plants", "15. Life on land", "Primary Research Articles", "plant diversity", "United States", "plant communities", "meta-analysis", "Environmental sciences", "Ecology", " evolutionary biology", "13. Climate action", "TEMPERATE FORESTS", "INVASIVE EARTHWORMS", "Introduced Species"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.13446"}, {"href": "https://doi.org/10.1111/gcb.13446"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.13446", "name": "item", "description": "10.1111/gcb.13446", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.13446"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-03T00:00:00Z"}}, {"id": "10.3168/jds.2010-4042", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:24:42Z", "type": "Journal Article", "created": "2011-05-25", "title": "Dietary Inclusion Of Diallyl Disulfide, Yucca Powder, Calcium Fumarate, An Extruded Linseed Product, Or Medium-Chain Fatty Acids Does Not Affect Methane Production In Lactating Dairy Cows", "description": "Two similar experiments were conducted to assess the effect of diallyl disulfide (DADS), yucca powder (YP), calcium fumarate (CAFU), an extruded linseed product (UNSAT), or a mixture of capric and caprylic acid (MCFA) on methane production, energy balance, and dairy cow performance. In experiment 1, a control diet (CON1) and diets supplemented with 56 mg of DADS/kg of dry matter (DM), 3g of YP/kg of DM, or 25 g of CAFU/kg of DM were evaluated. In experiment 2, an inert saturated fat source in the control diet (CON2) was exchanged isolipidically for an extruded linseed source (100g/kg of DM; UNSAT) or a mixture of C8:0 and C10:0 (MCFA; 20.3g/kg of DM). In experiment 2, a higher inclusion level of DADS (200mg/kg of DM) was also tested. Both experiments were conducted using 40 lactating Holstein-Friesian dairy cows. Cows were adapted to the diet for 12 d and were subsequently kept in respiration chambers for 5 d to evaluate methane production, diet digestibility, energy balance, and animal performance. Feed intake was restricted to avoid confounding effects of possible differences in ad libitum feed intake on methane production. Feed intake was, on average, 17.5 and 16.6 kg of DM/d in experiments 1 and 2, respectively. None of the additives reduced methane production in vivo. Methane production in experiment 1 was 450, 453, 446, and 423 g/d for CON1 and the diets supplemented with DADS, YP, and CAFU, respectively. In experiment 2, methane production was 371, 394, 388, and 386 g/d for CON2 and the diets supplemented with UNSAT, MCFA, and DADS, respectively. No effects of the additives on energy balance or neutral detergent fiber digestibility were observed. The addition of MCFA increased milk fat content (5.38% vs. 4.82% for control) and fat digestibility (78.5% vs. 59.8% for control), but did not affect milk yield or other milk components. The other products did not affect milk yield or composition. Results from these experiments emphasize the need to confirm methane reductions observed in vitro with in vivo data.", "keywords": ["production in-vitro", "ruminal fermentation", "0404 agricultural biotechnology", "linolenic acids", "rumen microbial fermentation", "Fumarates", "Flax", "Yucca", "Animals", "Lactation", "schidigera", "Disulfides", "essential oils", "beef-cattle", "2. Zero hunger", "garlic oil", "0402 animal and dairy science", "04 agricultural and veterinary sciences", "Diet", "Allyl Compounds", "Milk", "Dietary Supplements", "Animal Nutritional Physiological Phenomena", "Cattle", "Digestion", "Female", "Caprylates", "Energy Metabolism", "metabolism", "Decanoic Acids", "Methane", "metaanalysis"]}, "links": [{"href": "https://doi.org/10.3168/jds.2010-4042"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Dairy%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3168/jds.2010-4042", "name": "item", "description": "10.3168/jds.2010-4042", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3168/jds.2010-4042"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-06-01T00:00:00Z"}}, {"id": "10.5281/zenodo.14790778", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-06-25T16:28:00Z", "type": "Journal Article", "created": "2019-04-01", "title": "Coupled carbon and nitrogen losses in response to seven years of chronic warming in subarctic soils", "description": "Increasing temperatures may alter the stoichiometric demands of soil microbes and impair their capacity to stabilize carbon (C) and retain nitrogen (N), with critical consequences for the soil C and N storage at high latitude soils. Geothermally active areas in Iceland provided wide, continuous and stable gradients of\u00a0soil temperatures\u00a0to test this hypothesis. In order to characterize the stoichiometric demands of microbes from these subarctic soils, we incubated soils from ambient temperatures after the factorial addition of C, N and P substrates separately and in combination. In a second experiment, soils that had been exposed to different\u00a0in situ\u00a0warming intensities (+0, +0.5, +1.8, +3.4, +8.7, +15.9\u00a0\u00b0C above ambient) for seven years were incubated after the combined addition of C, N and P to evaluate the capacity of soil microbes to store and immobilize C and N at the different warming scenarios. The seven years of chronic soil warming triggered large and proportional soil C and N losses (4.1\u00a0\u00b1\u00a00.5% \u00b0C\u22121\u00a0of the stocks in unwarmed soils) from the upper 10\u202fcm of soil, with a predominant depletion of the physically accessible organic substrates that were weakly sorbed in\u00a0soil minerals\u00a0up to 8.7\u202f\u00b0C warming. Soil microbes met the increasing respiratory demands under conditions of low C accessibility at the expenses of a reduction of the standing biomass in warmer soils. This together with the strict microbial C:N stoichiometric demands also constrained their capacity of N retention, and increased the vulnerability of soil to N losses. Our findings suggest a strong control of\u00a0microbial physiology and C:N stoichiometric needs on the retention of soil N and on the resilience of soil C stocks from high-latitudes to warming, particularly during periods of vegetation dormancy and low C inputs.", "keywords": ["0301 basic medicine", "Microbial carbon and nutrients limitation", "Microbial biomass", "TERM", "03 medical and health sciences", "Temperature increase", "FOREST SOIL", "Substrate induced respiration", "ORGANIC-CARBON", "SDG 13 - Climate Action", "TEMPERATURE SENSITIVITY", "CYCLE", "106026 Ecosystem research", "METAANALYSIS", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "Nitrogen loss", "CLIMATE-CHANGE", "AVAILABILITY", "15. Life on land", "106026 \u00d6kosystemforschung", "13. Climate action", "SDG 13 \u2013 Ma\u00dfnahmen zum Klimaschutz", "Nitrogen immobilization", "FEEDBACKS", "106022 Microbiology", "PLANT BIOMASS"]}, "links": [{"href": "https://doi.org/10.5281/zenodo.14790778"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5281/zenodo.14790778", "name": "item", "description": "10.5281/zenodo.14790778", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5281/zenodo.14790778"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-07-01T00:00:00Z"}}, {"id": "11250/3025285", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:31:38Z", "type": "Journal Article", "created": "2022-04-19", "title": "Do Agricultural Advisory Services in Europe Have the Capacity to Support the Transition to Healthy Soils?", "description": "

The need to provide appropriate information, technical advice and facilitation to support farmers in transitioning towards healthy soils is increasingly clear, and the role of the Agricultural Advisory Services (AAS) in this is critical. However, the transformation of AAS (plurality, commercialisation, fragmentation, decentralisation) brings new challenges for delivering advice to support soil health management. This paper asks: To what extent do agricultural advisory services have the capacity to support the transition to healthy soils across Europe? Using the \u2018best fit\u2019 framework, analytical characteristics of the AAS relevant to the research question (governance structures, management, organisational and individual capacities) were identified. Analysis of 18 semi-structured expert interviews across 6 case study countries in Europe, selected to represent a range of contexts, was undertaken. Capacities to provide soil health management (SHM) advice are constrained by funding arrangements, limited adviser training and professional development, adviser motivations and professional cultures, all determined by institutional conditions. This has resulted in a narrowing down of access and content of soil advice and a reduced capacity to support the transition in farming to healthy soils. The extent to which emerging policy and market drivers incentivise enhanced capacities in AAS is an important area for future research.

", "keywords": ["S1", "KNOWLEDGE EXCHANGE", "agricultural advisers", "Environmental Studies", "0211 other engineering and technologies", "Environmental Sciences & Ecology", "sustainable soil management", "02 engineering and technology", "3301 Architecture", "4104 Environmental management", "SYSTEMS", "advice", "MANAGEMENT", "S589.7_Agricultural", "0502 Environmental Science and Management", "soil policy", "METAANALYSIS", "2. Zero hunger", "Science & Technology", "CHALLENGES", "soil health", "agricultural advisory services; soil health; governance; agricultural advisers; sustainable soil management; soil policy; advice", "S", "GOVERNANCE", "3304 Urban and regional planning", "Agriculture", "04 agricultural and veterinary sciences", "POLICY", "FIT", "governance", "agricultural advisory services", "0401 agriculture", " forestry", " and fisheries", "FARM ADVISERS", "FRAGMENTATION", "Life Sciences & Biomedicine"]}, "links": [{"href": "https://eprints.glos.ac.uk/10944/3/10944-Ingram-et-al-%282022%29-Do-Agricultural-Advisory-Services.pdf"}, {"href": "http://www.mdpi.com/2073-445X/11/5/599/pdf"}, {"href": "https://doi.org/11250/3025285"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Land", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11250/3025285", "name": "item", "description": "11250/3025285", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11250/3025285"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-04-19T00:00:00Z"}}, {"id": "10261/356392", "type": "Feature", "geometry": null, "properties": {"updated": "2026-06-25T16:31:13Z", "type": "Journal Article", "created": "2021-12-19", "title": "Stimulation of ammonia oxidizer and denitrifier abundances by nitrogen loading: Poor predictability for increased soil N2O emission", "description": "Abstract

Unprecedented nitrogen (N) inputs into terrestrial ecosystems have profoundly altered soil N cycling. Ammonia oxidizers and denitrifiers are the main producers of nitrous oxide (N2O), but it remains unclear how ammonia oxidizer and denitrifier abundances will respond to N loading and whether their responses can predict N\uffe2\uff80\uff90induced changes in soil N2O emission. By synthesizing 101 field studies worldwide, we showed that N loading significantly increased ammonia oxidizer abundance by 107% and denitrifier abundance by 45%. The increases in both ammonia oxidizer and denitrifier abundances were primarily explained by N loading form, and more specifically, organic N loading had stronger effects on their abundances than mineral N loading. Nitrogen loading increased soil N2O emission by 261%, whereas there was no clear relationship between changes in soil N2O emission and shifts in ammonia oxidizer and denitrifier abundances. Our field\uffe2\uff80\uff90based results challenge the laboratory\uffe2\uff80\uff90based hypothesis that increased ammonia oxidizer and denitrifier abundances by N loading would directly cause higher soil N2O emission. Instead, key abiotic factors (mean annual precipitation, soil pH, soil C:N ratio, and ecosystem type) explained N\uffe2\uff80\uff90induced changes in soil N2O emission. Altogether, these findings highlight the need for considering the roles of key abiotic factors in regulating soil N transformations under N loading to better understand the microbially mediated soil N2O emission.Arctic and alpine tundra ecosystems are large reservoirs of organic carbon1,2. Climate warming may stimulate ecosystem respiration and release carbon into the atmosphere3,4. The magnitude and persistency of this stimulation and the environmental mechanisms that drive its variation remain uncertain5\uffe2\uff80\uff937. This hampers the accuracy of global land carbon\uffe2\uff80\uff93climate feedback projections7,8. Here we synthesize 136 datasets from 56 open-top chamber in situ warming experiments located at 28 arctic and alpine tundra sites which have been running for less than 1\uffe2\uff80\uff89year up to 25\uffe2\uff80\uff89years. We show that a mean rise of 1.4\uffe2\uff80\uff89\uffc2\uffb0C [confidence interval (CI) 0.9\uffe2\uff80\uff932.0\uffe2\uff80\uff89\uffc2\uffb0C] in air and 0.4\uffe2\uff80\uff89\uffc2\uffb0C [CI 0.2\uffe2\uff80\uff930.7\uffe2\uff80\uff89\uffc2\uffb0C] in soil temperature results in an increase in growing season ecosystem respiration by 30% [CI 22\uffe2\uff80\uff9338%] (n\uffe2\uff80\uff89=\uffe2\uff80\uff89136). Our findings indicate that the stimulation of ecosystem respiration was due to increases in both plant-related and microbial respiration (n\uffe2\uff80\uff89=\uffe2\uff80\uff899) and continued for at least 25\uffe2\uff80\uff89years (n\uffe2\uff80\uff89=\uffe2\uff80\uff89136). The magnitude of the warming effects on respiration was driven by variation in warming-induced changes in local soil conditions, that is, changes in total nitrogen concentration and pH and by context-dependent spatial variation in these conditions, in particular total nitrogen concentration and the carbon:nitrogen ratio. Tundra sites with stronger nitrogen limitations and sites in which warming had stimulated plant and microbial nutrient turnover seemed particularly sensitive in their respiration response to warming. The results highlight the importance of local soil conditions and warming-induced changes therein for future climatic impacts on respiration.International initiatives are emphasizing the capture of atmospheric CO2 in soil organic C (SOC) to reduce the climatic footprint from agroecosystems. One approach to quantify the contribution of management practices towards that goal is through analysis of long-term experiments (LTEs). Our objectives were to analyze knowledge gained in literature reviews on SOC changes in LTEs, to evaluate the results regarding interactions with pedo-climatological factors, and to discuss disparities among reviews in data selection criteria. We summarized mean response ratios (RRs) and stock change rate (SCR) effect size indices from twenty reviews using paired comparisons (N). The highest RRs were found with manure applications (30%, N\uffe2\uff80\uff89=\uffe2\uff80\uff89418), followed by aboveground crop residue retention and the use of cover crops (9\uffe2\uff80\uff9310%, N\uffe2\uff80\uff89=\uffe2\uff80\uff89995 and 129), while the effect of nitrogen fertilization was lowest (6%, N\uffe2\uff80\uff89=\uffe2\uff80\uff89846). SCR for nitrogen fertilization exceeded that for aboveground crop residue retention (233 versus 117\uffc2\uffa0kg\uffc2\uffa0C\uffc2\uffa0ha\uffe2\uff88\uff921\uffc2\uffa0year\uffe2\uff88\uff921, N\uffe2\uff80\uff89=\uffe2\uff80\uff89183 and 279) and was highest for manure applications and cover crops (409 and 331\uffc2\uffa0kg\uffc2\uffa0C\uffc2\uffa0ha\uffe2\uff88\uff921\uffc2\uffa0year\uffe2\uff88\uff921, N\uffe2\uff80\uff89=\uffe2\uff80\uff89217 and 176). When data allows, we recommend calculating both RR and SCR because it improves the interpretation. Our synthesis shows that results are not always consistent among reviews and that interaction with texture and climate remain inconclusive. Selection criteria for study durations are highly variable, resulting in irregular conclusions for the effect of time on changes in SOC. We also discuss the relationships of SOC changes with yield and cropping systems, as well as conceptual problems when scaling-up results obtained from field studies to regional levels.Cropping practices have a great potential to improve soil quality through changes in soil biota. Yet the effects of these soil\uffe2\uff80\uff90improving cropping systems on soil fungal communities are not well known. Here, we analysed soil fungal communities using standardized measurements in 12 long\uffe2\uff80\uff90term experiments and 20 agricultural treatments across Europe. We were interested in whether the same practices (i.e., tillage, fertilization, organic amendments and cover crops) applied across different sites have predictable and repeatable effects on soil fungal communities and guilds. The fungal communities were very variable across sites located in different soil types and climatic regions. The arbuscular mycorrhizal fungi (AMF) were the fungal guild with most unique species in individual sites, whereas plant pathogenic fungi were most shared between the sites. The fungal communities responded to the cropping practices differently in different sites and only fertilization showed a consistent effect on AMF and plant pathogenic fungi, whereas the responses to tillage, cover crops and organic amendments were site, soil and crop\uffe2\uff80\uff90species specific. We further show that the crop yield is negatively affected by cropping practices aimed at improving soil health. Yet, we show that these practices have the potential to change the fungal communities and that change in plant pathogenic fungi and in AMF is linked to the yield. We further link the soil fungal community and guilds to soil abiotic characteristics and reveal that especially Mn, K, Mg and pH affect the composition of fungi across sites. In summary, we show that fungal communities vary considerably between sites and that there are no clear directional responses in fungi or fungal guilds across sites to soil\uffe2\uff80\uff90improving cropping systems, but that the responses vary based on soil abiotic conditions, crop type and climatic conditions.

Highlights

Soil fungi were analysed using standardized measurements in 12 long\uffe2\uff80\uff90term experiments and 20 agricultural treatments

Fungal communities responded to the cropping practices differently at different sites

Only reduced fertilization showed a consistent effect on AMF and plant pathogenic fungi, whereas the responses to tillage, cover crops and organic amendments were site specific.

Fungal community structure varied significantly between sites, crops and climate conditions; therefore, more cross\uffe2\uff80\uff90site studies are needed in order to manage beneficial soil fungi in agricultural systems.

Unprecedented nitrogen (N) inputs into terrestrial ecosystems have profoundly altered soil N cycling. Ammonia oxidizers and denitrifiers are the main producers of nitrous oxide (N2O), but it remains unclear how ammonia oxidizer and denitrifier abundances will respond to N loading and whether their responses can predict N\uffe2\uff80\uff90induced changes in soil N2O emission. By synthesizing 101 field studies worldwide, we showed that N loading significantly increased ammonia oxidizer abundance by 107% and denitrifier abundance by 45%. The increases in both ammonia oxidizer and denitrifier abundances were primarily explained by N loading form, and more specifically, organic N loading had stronger effects on their abundances than mineral N loading. Nitrogen loading increased soil N2O emission by 261%, whereas there was no clear relationship between changes in soil N2O emission and shifts in ammonia oxidizer and denitrifier abundances. Our field\uffe2\uff80\uff90based results challenge the laboratory\uffe2\uff80\uff90based hypothesis that increased ammonia oxidizer and denitrifier abundances by N loading would directly cause higher soil N2O emission. Instead, key abiotic factors (mean annual precipitation, soil pH, soil C:N ratio, and ecosystem type) explained N\uffe2\uff80\uff90induced changes in soil N2O emission. Altogether, these findings highlight the need for considering the roles of key abiotic factors in regulating soil N transformations under N loading to better understand the microbially mediated soil N2O emission.