{"type": "FeatureCollection", "features": [{"id": "10.1016/j.catena.2015.10.018", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:18Z", "type": "Journal Article", "created": "2015-10-26", "title": "Grazing Exclusion Significantly Improves Grassland Ecosystem C And N Pools In A Desert Steppe Of Northwest China", "description": "Abstract   Grazing exclusion is often implemented as an effective management practice to increase the sustainability of grassland ecosystems. However, it remains unclear if grazing exclusion can improve ecosystem services related to carbon (C) and nitrogen (N) sequestration in grassland ecosystems. We investigated the effects of 11\u00a0years of grazing exclusion on plant biomass and diversity, soil properties (pH, soil water content (SWC), bulk density (BD), soil organic carbon (SOC), total nitrogen (TN), and C/N ratio), and the C and N stocks of plants and soils in a desert grassland of Northwest China. Grazing exclusion improved plant aboveground biomass and diversity, as well as SWC, SOC, and TN contents, but lowered the belowground biomass, root/shoot ratio, pH, and BD. Moreover, grazing exclusion strongly influenced the C and N stocks of the ecosystem, and the annual mean ecosystem C and N sequestration rates were 0.47 and 0.09\u00a0Mg\u00a0ha \u2212\u00a01 \u00a0yr \u2212\u00a01 , respectively, over 11\u00a0years of grazing exclusion. Soil C stocks were most dynamic in the top 30\u00a0cm of the soil, and N stocks mainly changed in the top 20\u00a0cm after grazing exclusion. Our results indicated that grazing exclusion is an effective measurement on improving the ecosystem C and N pools in desert steppe of Northwest China.", "keywords": ["SOIL ORGANIC C", "0106 biological sciences", "Carbon Sequestration", "550", "MICROBIAL-COMMUNITY", "SPATIAL VARIABILITY", "PHYSICAL-PROPERTIES", "Soil Science", "01 natural sciences", "Soil Prosperities", "CENTRAL ARGENTINA", "CARBON STORAGE", "PLANT-COMMUNITIES", "Vegetation Characteristics", "580", "2. Zero hunger", "Science & Technology", "Multidisciplinary", "PRODUCTIVITY", "Nitrogen Sequestration", "Geology", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "INNER-MONGOLIA", "Grazing", "13. 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Because microbial growth in soil is limited by substrate inputs from plant production, we reasoned that changes in the amount and chemistry of these organic substrates could affect the composition of soil microbial com- munities and the cycling of N in soil. We studied microbial community composition and soil-N transformations beneath Populus tremuloides Michx. growing under experimental atmospheric CO2 (35.7 and 70.7 Pa) and soil-N-availability (low N 5 61 ng N\u00b7g 21 \u00b7d 21 and high N 5 319 ng N\u00b7g 21 \u00b7d 21 ) treatments. Atmospheric CO2 concentration was modified in large, open-top chambers, and we altered soil-N availability in open-bottom root boxes by mixing different proportions of A and C horizon material. We used phospholipid fatty-acid analysis to gain insight into microbial community composition and coupled this analysis to measurements of soil-N transformations using 15 N-pool dilution techniques. The infor- mation presented here is part of an integrated experiment designed to elucidate the phys- iological mechanisms controlling the flow of C and N in the plant-soil system. Our ob- jectives were (1) to determine whether changes in plant growth and tissue chemistry alter microbial community composition and soil-N cycling in response to increasing atmospheric CO2 and soil-N availability and (2) to integrate the results of our experiment into a synthesis of elevated atmospheric CO2 and the cycling of C and N in terrestrial ecosystems. After 2.5 growing seasons, microbial biomass, gross N mineralization, microbial im- mobilization, and nitrification (gross and net) were equivalent at ambient and elevated CO2, suggesting that increases in fine-root production and declines in fine-root N concentration were insufficient to alter the influence of native soil organic matter on microbial physiology; this was the case in both low- and high-N soil. Similarly, elevated CO2 did not alter the proportion of bacterial, actinomycetal, or fungal phospholipid fatty acids in low-N or high-N soil, indicating that changes in substrate input from greater plant growth under elevated CO2 did not alter microbial community composition. Our results differ from a substantial number of studies reporting increases and decreases in soil-N cycling under elevated CO 2. From our analysis, it appears that soil-N cycling responds to elevated atmospheric CO 2 in experimental situations where plant roots have fully colonized the soil and root-associated C inputs are sufficient to modify the influence of native soil organic matter on microbial physiology. In young developing ecosystems where plant roots have not fully exploited the soil, microbial metabolism appears to be regulated by relatively large pools of soil organic matter, rather than by the additional input of organic substrates under elevated CO 2.", "keywords": ["measurement-", "soil microorganisms", "Ecology and Evolutionary Biology", "nitrogen-: cycling-", "feedback", "microbial community composition", "techniques-", "Environmental-Sciences)", "01 natural sciences", "litter-plant", "biomass-", "gross and net", "124-38-9: CARBON DIOXIDE", "Spermatophytes-", "cycling-", "soil-organic-matter", "mineralization", "Spermatophyta-", "responses-", "phospholipid-fatty-acids", "2. Zero hunger", "Climatology- (Environmental-Sciences)", "Angiosperms-", "Angiospermae-", "Plants-", "global climate change", "microbial immobilization", "nutrient-", "Soil-Science", "6. 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