{"type": "FeatureCollection", "features": [{"id": "10.1039/c8en01378k", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:17:51Z", "type": "Journal Article", "created": "2019-04-15", "title": "Strategies for robust and accurate experimental approaches to quantify nanomaterial bioaccumulation across a broad range of organisms", "description": "<p>Strategies are provided for making robust measurements of engineered nanomaterial bioaccumulation across a broad range of organisms.</p>", "keywords": ["WALLED CARBON NANOTUBES", "Environmental Engineering", "Biomedical and Clinical Sciences", "TITANIUM-DIOXIDE NANOPARTICLES", "Medical Biotechnology", "ZINC-OXIDE NANOPARTICLES", "0211 other engineering and technologies", "Bioengineering", "02 engineering and technology", "PLASMA-MASS SPECTROMETRY", "SILVER NANOPARTICLES", "01 natural sciences", "Environmental Biotechnology", "ENGINEERED NANOMATERIALS", "GOLD NANOPARTICLES", "MUSSEL MYTILUS-EDULIS", "SINGLE-CELL LEVEL", "Life Science", "Generic health relevance", "Other Chemical Sciences", "PARTICLE ICP-MS", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://pubs.rsc.org/en/content/articlepdf/2019/EN/C8EN01378K"}, {"href": "https://escholarship.org/content/qt3ct0979c/qt3ct0979c.pdf"}, {"href": "https://doi.org/10.1039/c8en01378k"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%3A%20Nano", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1039/c8en01378k", "name": "item", "description": "10.1039/c8en01378k", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1039/c8en01378k"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-01-01T00:00:00Z"}}, {"id": "10.1007/s00216-022-03943-6", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:14:50Z", "type": "Journal Article", "created": "2022-02-09", "title": "Systematic identification of trimethoprim metabolites in lettuce", "description": "Abstract<p>Antibiotics are some of the most widely used drugs. Their release in the environment is of great concern since their consumption is a major factor for antibiotic resistance, one of the most important threats to human health. Their occurrence and fate in agricultural systems have been extensively investigated in recent years. Yet whilst their biotic and abiotic degradation pathways have been thoroughly researched, their biotransformation pathways in plants are less understood, such as in case of trimethoprim. Although trimethoprim has been reported in the environment, its fate in higher plants still remains unknown. A bench-scale experiment was performed and 30 trimethoprim metabolites were identified in lettuce (Lactuca sativa L.), of which 5 belong to phase I and 25 to phase II. Data mining yielded a list of 1018 ions as possible metabolite candidates, which was filtered to a final list of 87 candidates. Molecular structures were assigned for 19 compounds, including 14 TMP metabolites reported for the first time. Alongside well-known biotransformation pathways in plants, additional novel pathways were suggested, namely, conjugation with sesquiterpene lactones, and abscisic acid as a part of phase II of plant metabolism. The results obtained offer insight into the variety of phase II conjugates and may serve as a guideline for studying the metabolization of other chemicals that share a similar molecular structure or functional groups with trimethoprim. Finally, the toxicity and potential contribution of the identified metabolites to the selective pressure on antibiotic resistance genes and bacterial communities via residual antimicrobial activity were evaluated.</p>", "keywords": ["2. Zero hunger", "High-resolution mass spectrometry", "Phytochemicals", "0211 other engineering and technologies", "02 engineering and technology", "01 natural sciences", "Trimethoprim", "Anti-Bacterial Agents", "3. Good health", "Conjugates", "Antibiotics", "Non-target screening", "Humans", "Plant metabolites", "Biotransformation", "Research Paper", "Lactuca", "0105 earth and related environmental sciences"], "contacts": [{"organization": "Tadi\u0107, \u0110or\u0111e, Gramblicka, Michal, Mistrik, Robert, Bayona, Josep Maria,", "roles": ["creator"]}]}, "links": [{"href": "https://link.springer.com/content/pdf/10.1007/s00216-022-03943-6.pdf"}, {"href": "https://doi.org/10.1007/s00216-022-03943-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Analytical%20and%20Bioanalytical%20Chemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00216-022-03943-6", "name": "item", "description": "10.1007/s00216-022-03943-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00216-022-03943-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-02-09T00:00:00Z"}}, {"id": "10.1002/rcm.1184", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-07-28T16:14:38Z", "type": "Journal Article", "created": "2003-12-02", "title": "Quantification Of Priming And Co2 Respiration Sources Following Slurry-C Incorporation Into Two Grassland Soils With Different C Content", "description": "Abstract<p>The fate of incorporated slurry\uffe2\uff80\uff90C was examined in a laboratory experiment using two UK grassland soils, i.e. a Pelostagnogley (5.1 %C) and a Brown Earth (2.3 %C). C3 and C4 slurries were incorporated into these two wet\uffe2\uff80\uff90sieved (C3) soils (from 4\uffe2\uff80\uff9310 cm depth). Gas samples were collected 0.2, 1, 2, 3, 4, 6, 9, 20, 30 and 40 days after slurry application and analyzed for CO2 concentration and \uffce\uffb413C content. Slurry incorporation into the soil strongly increased soil CO2 respiration compared with the unamended soil. Total (40 day) cumulative CO2 flux was higher for the Pelostagnogley than the Brown Earth. The 13C natural abundance tracer technique enabled quantification of the sources of respired CO2 and priming effects (days 0\uffe2\uff80\uff939). Proportionally more slurry\uffe2\uff80\uff90derived C was respired from the Pelostagnogley (46%) than the Brown Earth (36%). The incorporated slurry\uffe2\uff80\uff90C was lost twice as fast as the native soil C in both soils. Slurry incorporation induced a priming effect, i.e. additional release of soil\uffe2\uff80\uff90derived C, most pronounced in the Pelostagnogley (highest C content). The majority of respired soil\uffe2\uff80\uff90derived C (&gt;70%) was primed C. The study indicated that potential reductions in ammonia volatilisation following slurry injection to grasslands might be negated by enhanced loss of primed soil C (i.e. pollution swapping). Copyright \uffc2\uffa9 2003 John Wiley &amp; Sons, Ltd.</p>", "keywords": ["2. Zero hunger", "Carbon Isotopes", "Agriculture", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "Poaceae", "Waste Disposal", " Fluid", "Carbon", "Mass Spectrometry", "6. Clean water", "Soil", "England", "Animals", "0401 agriculture", " forestry", " and fisheries", "Cattle"]}, "links": [{"href": "https://doi.org/10.1002/rcm.1184"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Rapid%20Communications%20in%20Mass%20Spectrometry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/rcm.1184", "name": "item", "description": "10.1002/rcm.1184", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/rcm.1184"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2003-10-30T00:00:00Z"}}, {"id": "10.1007/s11130-020-00799-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:15:26Z", "type": "Journal Article", "created": "2020-02-03", "title": "Profile and Content of Residual Alkaloids in Ten Ecotypes of Lupinus mutabilis Sweet after Aqueous Debittering Process", "description": "Abstract<p>The evaluation of the level of alkaloids in edible Lupinus species is crucial from a food safety point of view. Debittering of lupin seeds has a long history; however, the control of the level of alkaloids after processing the seeds is typically only evaluated by changes in the bitter taste. The aim of this study was to evaluate the profile and residual levels of quinolizidine alkaloids (QA) in (Lupinus mutabilis Sweet) after aqueous debittering process. Samples from 10 ecotypes from different areas of Peru were analyzed before and after the process. Based on results obtained by gas chromatography and mass spectrometry, from eight alkaloids identified before the debittering process, only small amounts of lupanine (avg. 0.0012\uffc2\uffa0g/100\uffc2\uffa0g DM) and sparteine (avg. 0.0014\uffc2\uffa0g/100\uffc2\uffa0g DM) remained in the seeds after the debittering process, and no other alkaloids were identified. The aqueous debittering process reduced the content of alkaloids to levels far below the maximal level allowed by international regulations (\uffe2\uff89\uffa4 0.2\uffc2\uffa0g/kg DM).</p>", "keywords": ["0301 basic medicine", "Lupin Seeds", "Sparteine", "Organic chemistry", "Propiedades fisicoqu\u00edmicas", "Plant Science", "Gas Chromatography-Mass Spectrometry", "Evolution and Nutritional Properties of Lupin Seeds", "Agricultural and Biological Sciences", "Food science", "Per\u00fa", "03 medical and health sciences", "Deshidrataci\u00f3n acuosa", "Alkaloids", "Secado", "Tarwi", "https://purl.org/pe-repo/ocde/ford#2.11.01", "Granos", "Composici\u00f3n qu\u00edmica", "ta116", "Biology", "Ecotipos", "Ecology", " Evolution", " Behavior and Systematics", "Ecotype", "2. Zero hunger", "Original Paper", "0303 health sciences", "Rendimiento", "Procesamiento", "Evaluaci\u00f3n", "ta1183", "An\u00e1lisis organol\u00e9ptico", "ta1182", "Botany", "Life Sciences", "Diversity and Applications of Cyperus Species", "Lupinus", "Chocho", "Chemistry", "Contenido proteico", "Evolution and Ecology of Endophyte-Grass Symbiosis", "Taste", "Seeds", "Lupinus mutabilis"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s11130-020-00799-y.pdf"}, {"href": "https://doi.org/10.1007/s11130-020-00799-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Foods%20for%20Human%20Nutrition", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11130-020-00799-y", "name": "item", "description": "10.1007/s11130-020-00799-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11130-020-00799-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-02-03T00:00:00Z"}}, {"id": "10.1007/s00425-017-2647-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:14:58Z", "type": "Journal Article", "created": "2017-01-04", "title": "The cost of surviving nitrogen excess: energy and protein demand in the lichen Cladonia portentosa as revealed by proteomic analysis", "description": "Different nitrogen forms affect different metabolic pathways in lichens. In particular, the most relevant changes in protein expression were observed in the fungal partner, with NO 3- mostly affecting the energetic metabolism and NH 4+ affecting transport and regulation of proteins and the energetic metabolism much more than NO 3- did. Excess deposition of reactive nitrogen is a well-known agent of stress for lichens, but which symbiont is most affected and how, remains a mystery. Using proteomics can expand our understanding of stress effects on lichens. We investigated the effects of different doses and forms of reactive nitrogen, with and without supplementary phosphorus and potassium, on the proteome of the lichen Cladonia portentosa growing in a 'real-world' simulation of nitrogen deposition. Protein expression changed with the nitrogen treatments but mostly in the fungal partner, with NO3- mainly affecting the energetic metabolism and NH4+ also affecting the protein synthesis machinery. The photobiont mainly responded overexpressing proteins involved in energy production. This suggests that in response to nitrogen stress, the photobiont mainly supports the defensive mechanisms initiated by the mycobiont with an increased energy production. Such surplus energy is then used by the cell to maintain functionality in the presence of NO3-, while a futile cycle of protein production can be hypothesized to be induced by NH4+ excess. External supply of potassium and phosphorus influenced differently the responses of particular enzymes, likely reflecting the many processes in which potassium exerts a regulatory function.", "keywords": ["Chlorophyll", "Proteomics", "0301 basic medicine", "570", "mycobiont", "Lichens", "Nitrogen", "Cell Respiration", "Nitrate", "Mass Spectrometry", "Molecular mechanism", "03 medical and health sciences", "nitrate", "Ammonia", "Electrophoresis", " Gel", " Two-Dimensional", "Photosynthesis", "Ammonium", " Molecular mechanism", " Mycobiont", " Nitrate", " Photobiont", " Stress response", "Ammonium; Molecular mechanism; Mycobiont; Nitrate; Photobiont; Stress response; Genetics; Plant Science", "0303 health sciences", "Nitrates", "Stress response", "Chlorophyll A", "stress response", "Mycobiont", "ammonium", "Photobiont", "photobiont", "molecular mechanism", "Energy Metabolism", "Ammonium"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s00425-017-2647-2.pdf"}, {"href": "https://doi.org/10.1007/s00425-017-2647-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Planta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00425-017-2647-2", "name": "item", "description": "10.1007/s00425-017-2647-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00425-017-2647-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-04T00:00:00Z"}}, {"id": "10.1007/s10533-008-9257-9", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:15:10Z", "type": "Journal Article", "created": "2008-11-11", "title": "Nitrogen Deposition Effects On Soil Organic Matter Chemistry Are Linked To Variation In Enzymes, Ecosystems And Size Fractions", "description": "Recent research has dramatically advanced our understanding of soil organic matter chemistry and the role of N in some organic matter transformations, but the effects of N deposition on soil C dynamics remain difficult to anticipate. We examined soil organic matter chemistry and enzyme kinetics in three size fractions (>250 \u03bcm, 63\u2013250 \u03bcm, and  250 \u03bcm fraction of the sugar maple\u2013basswood ecosystem from 0.9 to 3.3 but there were no changes in other size classes or in the black oak\u2013white oak ecosystem. Third, simulated N deposition increased the ratio of lignin derivatives to N-bearing compounds in the 63\u2013250 and >250 \u03bcm fractions in both ecosystems but not in the  63 \u03bcm) where there were multiple correlations between oxidative enzyme activities and concentrations of lignin derivatives and between glycanolytic enzyme activities and concentrations of carbohydrates. Within silt-clay fractions (<63 \u03bcm), these enzyme-substrate correlations were attenuated by interactions with particle surfaces. Our results demonstrate that variation in enzyme activity resulting from atmospheric N deposition is directly linked to changes in soil organic matter chemistry, particularly those that occur within coarse soil size fractions.", "keywords": ["Carbon structure", "Soil organic matter", "13. Climate action", "Pyrolysis gas chromatography/mass spectrometry", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "Biogeochemistry", "15. Life on land", "Nitrogen deposition", "Enzymes"]}, "links": [{"href": "https://doi.org/10.1007/s10533-008-9257-9"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biogeochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10533-008-9257-9", "name": "item", "description": "10.1007/s10533-008-9257-9", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10533-008-9257-9"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2008-10-01T00:00:00Z"}}, {"id": "10.1007/s10646-012-0988-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:15:12Z", "type": "Journal Article", "created": "2012-08-25", "title": "Nematodes As Bioindicators Of Soil Degradation Due To Heavy Metals", "description": "The effect of distance from a heavy metal pollution source on the soil nematode community was investigated on four sampling sites along an 4 km transect originating at the Kovohuty a.s. Krompachy (pollution source). The soil nematode communities were exposed to heavy metal influence directly and through soil properties changes. We quantified the relative effects of total and mobile fraction of metals (As, Cd, Cr, Cu, Pb, and Zn) on soil ecosystem using the nematode community structure (trophic and c-p groups,) and ecological indices (Richness of genera, H', MI2-5, etc.). Pollution effects on the community structure of soil free living nematodes was found to be the highest near the pollution source, with relatively low population density and domination of insensitive taxa. A decrease in heavy metals contents along the transect was linked with an increase in complexity of nematode community. The majority of used indices (MI2-5, SI, H') negatively correlated (P < 0.05 or P < 0.01) with heavy metals content and were sensitive to soil ecosystem disturbance. Contamination by heavy metals has negatively affected the soil environment, which resulted in nematode community structure and ecological indices changes. Results showed that the free-living nematodes are useful tools for bioindication of contamination and could be used as an alternative to the common approaches based on chemical methods.", "keywords": ["Population Density", "Slovakia", "Nematoda", "Industrial Waste", "Environmental Exposure", "04 agricultural and veterinary sciences", "15. Life on land", "Biota", "01 natural sciences", "Mass Spectrometry", "13. Climate action", "Metals", " Heavy", "Metallurgy", "Animals", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Environmental Monitoring", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1007/s10646-012-0988-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecotoxicology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s10646-012-0988-y", "name": "item", "description": "10.1007/s10646-012-0988-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s10646-012-0988-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-08-26T00:00:00Z"}}, {"id": "10.1007/s11356-024-34957-5", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:15:29Z", "type": "Journal Article", "created": "2024-09-16", "title": "Beekeeping breakthrough: unveiling hive health with a portable membrane inlet mass spectrometry detection method", "description": "Supporting bee populations is essential considering threats posed by human activities like pesticide usage and habitat destruction. However, the current methods for monitoring and analyzing beehives and their surrounding environments are invasive, complex, and time-consuming. These methods often rely heavily on laboratory analyses, making them difficult to implement independently in the field. This study explores the application of portable membrane inlet mass spectrometer (MIMS) for noninvasive hive analysis, demonstrating its ability to detect various compounds indicative of hive conditions and environmental stressors. In addition to the expected compounds found in beehives, such as \u03b1-bergamotene, hexadecanoic acid, heptadecane, hexadecanamide,\u00a0\u03b1-bisabolol-, 9-octadecenamide, (Z)\u2009\u2212\u2009, and benzaldehyde, unexpected compounds, pollutants, like indane (polycyclic aromatic hydrocarbon) and carbofuran (pesticide), were also detected. The MIMS detection method provides rapid, accurate, and real-time results, making it suitable for preventive measures against bee diseases and integral to environmental biomonitoring. This integration of technology represents a significant advancement in bee conservation efforts, offering hope for the future of both bees and ecosystems.", "keywords": ["Animals", "Bees", "Beekeeping", "Mass Spectrometry", "Research Article", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10.1007/s11356-024-34957-5"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20and%20Pollution%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s11356-024-34957-5", "name": "item", "description": "10.1007/s11356-024-34957-5", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s11356-024-34957-5"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-09-16T00:00:00Z"}}, {"id": "10.1016/j.aca.2023.341718", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:15:36Z", "type": "Journal Article", "created": "2023-08-15", "title": "Surveying the mugineic acid family: Ion mobility \u2013 quadrupole time-of-flight mass spectrometry (IM-QTOFMS) characterization and tandem mass spectrometry (LC-ESI-MS/MS) quantification of all eight naturally occurring phytosiderophores", "description": "Phytosiderophores (PS) are root exudates released by grass species (Poaceae) that play a pivotal role in iron (Fe) plant nutrition. A direct determination of PS in biological samples is of paramount importance in understanding micronutrient acquisition mediated by PS. To date, eight plant-born PS have been identified; however, no analytical procedure is currently available to quantify all eight PS simultaneously with high analytical confidence. With access to the full set of PS standards for the first time, we report comprehensive methods to both fully characterize (IM-QTOFMS) and quantify (LC-ESI-MS/MS) all eight naturally occurring PS belonging to the mugineic acid family. The quantitative method was fully validated, yielding linear results for all eight analytes, and no unwanted interferences with soil and plant matrices were observed. LOD and LOQ values determined for each PS were below 11 and 35\u00a0nmol\u00a0L-1, respectively. The method's precision under reproducibility conditions (intra- and inter-day) of measurement was less than 2.5% RSD for all analytes. Additionally, all PS were annotated with high-resolution mass spectrometric fragment spectra and further characterized via drift tube ion mobility-mass spectrometry. The collision cross-sections obtained for primary ion species yielded a valuable database for future research focused on in-depth PS studies. The new quantitative method was applied to analyse root exudates from Fe-controlled and deficient barley, oat, rye, and sorghum plants. All eight PS, including mugineic acid (MA), 3'-hydroxymugineic acid (HMA), 3'-epi-hydroxymugineic acid (epi-HMA), hydroxyavenic acid (HAVA), deoxymugineic acid (DMA), 3'-hydroxydeoxymugineic acid (HDMA), 3'-epi-hydroxydeoxymugineic acid (epi-HDMA) and avenic acid (AVA) were for the first time successfully identified and quantified in root exudates of various graminaceous plants using a single analytical procedure. These newly developed methods can be applied to studies aimed at improving crop yield and micronutrient grain content for food consumption via plant-based biofortification.", "keywords": ["Tandem Mass Spectrometry", "Reproducibility of Results", "Micronutrients", "Poaceae", "Edible Grain"]}, "links": [{"href": "https://doi.org/10.1016/j.aca.2023.341718"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Analytica%20Chimica%20Acta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.aca.2023.341718", "name": "item", "description": "10.1016/j.aca.2023.341718", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.aca.2023.341718"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-01T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2011.05.052", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:16:04Z", "type": "Journal Article", "created": "2011-06-26", "title": "Poor Efficacy Of Herbicides In Biochar-Amended Soils As Affected By Their Chemistry And Mode Of Action", "description": "We evaluated wheat straw biochar produced at 450\u00b0C for its ability to influence bioavailability and persistence of two commonly used herbicides (atrazine and trifluralin) with different modes of action (photosynthesis versus root tip mitosis inhibitors) in two contrasting soils. The biochar was added to soils at 0%, 0.5% and 1.0% (w/w) and the herbicides were applied to those soil-biochar mixes at nil, half, full, two times, and four times, the recommended dosage (H(4)). Annual ryegrass (Lolium rigidum) was grown in biochar amended soils for 1 month. Biochar had a positive impact on ryegrass survival rate and above-ground biomass at most of the application rates, and particularly at H(4). Within any given biochar treatment, increasing herbicide application decreased the survival rate and fresh weight of above-ground biomass. Biomass production across the biochar treatment gradient significantly differed (p<0.01) and was more pronounced in the case of atrazine than trifluralin. For example, the dose-response analysis showed that in the presence of 1% biochar in soil, the value of GR(50) (i.e. the dose required to reduce weed biomass by 50%) for atrazine increased by 3.5 times, whereas it increased only by a factor of 1.6 in the case of trifluralin. The combination of the chemical properties and the mode of action governed the extent of biochar-induced reduction in bioavailability of herbicides. The greater biomass of ryegrass in the soil containing the highest biochar (despite having the highest herbicide residues) demonstrates decreased bioavailability of the chemicals caused by the wheat straw biochar. This work clearly demonstrates decreased efficacy of herbicides in biochar amended soils. The role played by herbicide chemistry and mode of action will have major implications in choosing the appropriate application rates for biochar amended soils.", "keywords": ["Carbon sequestration", "2. Zero hunger", "Bioavailability", "Herbicides", "Bound residue", "Weed control", "01 natural sciences", "630", "Gas Chromatography-Mass Spectrometry", "6. Clean water", "Trifluralin", "Persistence", "Biochar", "Soil", "Charcoal", "Lolium", "Atrazine", "Biomass", "Environmental Restoration and Remediation", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2011.05.052"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2011.05.052", "name": "item", "description": "10.1016/j.chemosphere.2011.05.052", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2011.05.052"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-09-01T00:00:00Z"}}, {"id": "10.1016/j.chemosphere.2014.06.024", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:16:04Z", "type": "Journal Article", "created": "2014-07-08", "title": "Combining Phytoextraction And Biochar Addition Improves Soil Biochemical Properties In A Soil Contaminated With Cd", "description": "The main goal of phytoremediation is to improve ecosystem functioning. Soil biochemical properties are considered as effective indicators of soil quality and are sensitive to various environmental stresses, including heavy metal contamination. The biochemical response in a soil contaminated with cadmium was tested after several treatments aimed to reduce heavy metal availability including liming, biochar addition and phytoextraction using Amaranthus tricolor L. Two biochars were added to the soil: eucalyptus pyrolysed at 600 \u00b0C (EB) and poultry litter at 400 \u00b0C (PLB). Two liming treatments were chosen with the aim of bringing soil pH to the same values as in the treatments EB and PLB. The properties studied included soil microbial biomass C, soil respiration and the activities of invertase, \u03b2-glucosidase, \u03b2-glucosaminidase, urease and phosphomonoesterase. Both phytoremediation and biochar addition improved soil biochemical properties, although results were enzyme specific. For biochar addition these changes were partly, but not exclusively, mediated by alterations in soil pH. A careful choice of biochar must be undertaken to optimize the remediation process from the point of view of metal phytoextraction and soil biological activity.", "keywords": ["China", "Eucalyptus", "Amaranthus", "Oxides", "04 agricultural and veterinary sciences", "Calcium Compounds", "15. Life on land", "01 natural sciences", "Gas Chromatography-Mass Spectrometry", "Mass Spectrometry", "6. Clean water", "3. Good health", "Soil", "Biodegradation", " Environmental", "Charcoal", "Soil Pollutants", "0401 agriculture", " forestry", " and fisheries", "Cadmium", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.chemosphere.2014.06.024"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.chemosphere.2014.06.024", "name": "item", "description": "10.1016/j.chemosphere.2014.06.024", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.chemosphere.2014.06.024"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-01-01T00:00:00Z"}}, {"id": "10.1016/j.jhazmat.2024.136780", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:16:43Z", "type": "Journal Article", "created": "2024-12-05", "title": "Sensitive and accurate determination of 32 PFAS in human serum using online SPE-UHPLC-HRMS", "description": "Per- and polyfluoroalkyl substances' (PFAS) extreme persistence has been linked to many adverse effects on human health including increased risk of certain cancers. This study presents the development and validation of a new, highly sensitive method for the quantification of 32 PFAS in human serum using online solid-phase extraction (SPE) coupled with ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Legacy and emerging PFAS were targeted. Main steps of sample pretreatment include protein precipitation (PP), pellet rinsing, centrifugation, preconcentration through solvent evaporation, and online SPE using a weak anion-exchange polymeric sorbent. The PP and pellet-rinsing procedures were optimized through a comprehensive exploration of solvent combinations. Following this, a pretreatment that offers the best compromise for the targeted PFAS was identified using principal component analysis. The method demonstrated excellent linearity (R\u00b2 = 0.977-0.997) with limits of quantification ranging from 8.9 to 27\u00a0ng/L, 5 to 15 times lower than previous methods. Precision (intraday 2.6-14.0\u00a0% and interday 1.3-11.0\u00a0% relative standard deviation) and accuracy (recoveries 72.7-106\u00a0%) were robust. The method was validated in accordance with ISO/IEC 17025 and successfully applied to five human serum samples, confirming its suitability for high-throughput profiling of PFAS in biomonitoring studies. This method is the first to use online SPE for the simultaneous determination of a broad range of PFAS, including ether congeners such as perfluoro(2-ethoxyethane) sulfonic acid and Nafion byproduct 2. Furthermore, control charts were employed to assess instrument performance during routine analysis and implement necessary actions.", "keywords": ["Human biomonitoring", "Fluorocarbons", "PFAS", "biomonitoring", "Method development", "Solid Phase Extraction", "628", "Humans", "Serum pretreatment", "High resolution mass spectrometry", "Chromatography", " High Pressure Liquid", "Mass Spectrometry", "543"]}, "links": [{"href": "https://iris.univr.it/bitstream/11562/1159353/1/2025%20Sensitive%20and%20accurate%20determination%20of%2032%20PFAS%20in%20human%20serum%20using%20SPE-UHPLC-HRMS.pdf"}, {"href": "https://doi.org/10.1016/j.jhazmat.2024.136780"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jhazmat.2024.136780", "name": "item", "description": "10.1016/j.jhazmat.2024.136780", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jhazmat.2024.136780"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-03-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2020.114002", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:16:16Z", "type": "Journal Article", "created": "2020-01-21", "title": "Elucidating biotransformation pathways of ofloxacin in lettuce (Lactuca sativa L)", "description": "Antibiotics can be uptaken by plants from soil desorption or directly from irrigation water, but their metabolization pathways in plants are largely unknown. In this paper, an analytical workflow based on high-resolution mass spectrometry was applied for the systematic identification of biotransformation products of ofloxacin in lettuce. The targeted metabolites were selected by comparing the mass chromatograms of exposed with control samples using an advanced spectra-processing method (Fragment Ion Search). The innovative methodology presented allowed us to identify a total of 11 metabolites, including 5 ofloxacin metabolites that are being reported for the first time in plants. Accordingly, major transformation pathways were proposed revealing insight into how ofloxacin and related chemicals are metabolized in lettuce. Furthermore, the influence of biotransformation on potential residual antimicrobial activity of identified compounds was discussed. Human exposure to antibiotics at doses below the minimum inhibitory concentrations is crucial in human risk assessment, including food ingestion; however, in the case of ofloxacin presented results reveal that plant metabolites should also be considered so as not to underestimate their risk.", "keywords": ["High-resolution mass spectrometry", "Ofloxacin", "0211 other engineering and technologies", "02 engineering and technology", "Biotransformation pathways", "01 natural sciences", "6. Clean water", "Anti-Bacterial Agents", "Antibiotics", "Humans", "Plant metabolites", "Biotransformation", "Lactuca", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2020.114002"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2020.114002", "name": "item", "description": "10.1016/j.envpol.2020.114002", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2020.114002"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2021.117927", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:16:16Z", "type": "Journal Article", "created": "2021-08-07", "title": "Groundwater antibiotic pollution and its relationship with dissolved organic matter: Identification and environmental implications", "description": "The occurrence of veterinary antibiotics and hydro-chemical parameters in eleven natural springs in a livestock production area is evaluated, jointly with the characterization of their DOM fingerprint by Orbitrap HRMS. Tetracycline and sulfonamide antibiotics were ubiquitous in all sites, and they were detected at low ng L-1 concentrations, except for doxycycline, that was present at \u03bcg L-1 in one location. DOM analysis revealed that most molecular formulas were CHO compounds (49 %-68\u00a0%), with a remarkable percentage containing nitrogen and sulphur (16 %-23\u00a0% and 11 %-24\u00a0%, respectively). Major DOM components were phenolic and highly unsaturated compounds (~90\u00a0%), typical for soil-derived organic matter, while approximately 11\u00a0% were unsaturated aliphatic, suggesting that springs may be susceptible to anthropogenic contamination sources. Comparing the DOM fingerprint among sites, the spring showing the most different profile was the one with surface water interaction and characterized by having lower CHO and higher CHOS formulas and aliphatic compounds. Correlations between antibiotics and DOM showed that tetracyclines positively correlate with unsaturated oxygen-rich substances, while sulfonamides relate with aliphatic and unsaturated oxygen-poor compounds. This indicates that the fate of different antibiotics will be controlled by the type of DOM present in groundwater.", "keywords": ["High-resolution mass spectrometry", "550", "Contaminants emergents en l'aigua", "Antibi\u00f2tics", "02 engineering and technology", "01 natural sciences", "630", "Soil", "Antibiotics", "Co-transport", "Groundwater -- Pollution", "Dissolved organic matter", "Groundwater", "0105 earth and related environmental sciences", "2. Zero hunger", "[SDU.OCEAN]Sciences of the Universe [physics]/Ocean", "Emerging contaminants in water", "Atmosphere", "[SDU.OCEAN] Sciences of the Universe [physics]/Ocean", " Atmosphere", "[SDU.ENVI] Sciences of the Universe [physics]/Continental interfaces", " environment", "6. Clean water", "Anti-Bacterial Agents", "Water quality", "13. Climate action", "Aig\u00fces subterr\u00e0nies -- Contaminaci\u00f3", "[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces", "0210 nano-technology", "environment", "Water Pollutants", " Chemical", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2021.117927"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2021.117927", "name": "item", "description": "10.1016/j.envpol.2021.117927", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2021.117927"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-11-01T00:00:00Z"}}, {"id": "10.1155/2018/9736547", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:19:11Z", "type": "Journal Article", "created": "2018-05-09", "title": "Chronological Classification of Ancient Mortars Employing Spectroscopy and Spectrometry Techniques: Sagunto (Valencia, Spain) Case", "description": "<p>Forty-two mortar samples, from two archaeological excavations located in Sagunto (Valencian Community, Spain), were analysed by both portable energy dispersive X-ray fluorescence spectroscopy (pED-XRF) and inductively coupled plasma mass spectrometry (ICP-MS) to determine major and minor elements and traces including rare earth elements (REEs). Collected data were crossed with those previously obtained from Sagunto Castle mortars, and principal component analysis (PCA) was applied to discriminate the construction phases of the unearthed buildings. REE permitted to ascribe most of the masonries to the Roman Imperial period. Moreover, a statistical model was built by employing partial least squares discriminant analysis (PLS-DA) in order to classify the mortars from Roman Imperial period and from Islamic period due to the problematic overlapping between these two phases. Results confirmed the effectiveness of the developed indirect chronology method, based on REE data, to discriminate among historic mortars from different construction periods on a wide scale including different Sagunto archaeological sites.</p>", "keywords": ["2. Zero hunger", "0601 history and archaeology", "QC350-467", "06 humanities and the arts", "Optics. Light", "energy dispersive X-ray fluorescence spectroscopy (pED-XRF); inductively coupled plasma mass spectrometry (ICP-MS)", "Analytical Chemistry; Atomic and Molecular Physics", " and Optics; Spectroscopy"]}, "links": [{"href": "https://eprints.whiterose.ac.uk/130462/1/9736547.pdf"}, {"href": "https://iris.unica.it/bitstream/11584/248342/2/Ramacciotti%20et%20al%202018.pdf"}, {"href": "https://arpi.unipi.it/bitstream/11568/935316/1/P101%20Chronological%20Classification%20of%20Ancient%20Mortars.pdf"}, {"href": "https://doi.org/10.1155/2018/9736547"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Spectroscopy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1155/2018/9736547", "name": "item", "description": "10.1155/2018/9736547", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1155/2018/9736547"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-01-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2013.03.090", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:16:51Z", "type": "Journal Article", "created": "2013-04-24", "title": "Microbial Utilisation Of Biochar-Derived Carbon", "description": "Whilst largely considered an inert material, biochar has been documented to contain a small yet significant fraction of microbially available labile organic carbon (C). Biochar addition to soil has also been reported to alter soil microbial community structure, and to both stimulate and retard the decomposition of native soil organic matter (SOM). We conducted a short-term incubation experiment using two (13)C-labelled biochars produced from wheat or eucalypt shoots, which were incorporated in an aridic arenosol to examine the fate of the labile fraction of biochar-C through the microbial community. This was achieved using compound specific isotopic analysis (CSIA) of phospholipid fatty acids (PLFAs). A proportion of the biologically-available fraction of both biochars was rapidly (within three days) utilised by gram positive bacteria. There was a sharp peak in CO2 evolution shortly after biochar addition, resulting from rapid turnover of labile C components in biochars and through positive priming of native SOM. Our results demonstrate that this CO2 evolution was at least partially microbially mediated, and that biochar application to soil can cause significant and rapid changes in the soil microbial community; likely due to addition of labile C and increases in soil pH.", "keywords": ["Carbon sequestration", "[SDE] Environmental Sciences", "Carbon Sequestration", "Chromatography", " Gas", "Magnetic Resonance Spectroscopy", "550", "short term", "[SDV]Life Sciences [q-bio]", "growth", "black carbon", "Char", "01 natural sciences", "630", "Mass Spectrometry", "c 13 plfa", "Black carbon", "soil organic matter", "Soil Pollutants", "mineralization", "Organic carbon", "Phospholipids", "Soil Microbiology", "char", "0105 earth and related environmental sciences", "2. Zero hunger", "Carbon Isotopes", "decomposition", "wheat straw", "biomass", "organic carbon", "Fatty Acids", "Western Australia", "04 agricultural and veterinary sciences", "Carbon Dioxide", "15. Life on land", "540", "pyrolysis", "forest soil", "carbon sequestration", "Carbon", "[SDV] Life Sciences [q-bio]", "Charcoal", "[SDE]Environmental Sciences", "0401 agriculture", " forestry", " and fisheries", "community structure", "\u00b9\u00b3C-PLFA", "Pyrolysis"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2013.03.090"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2013.03.090", "name": "item", "description": "10.1016/j.scitotenv.2013.03.090", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2013.03.090"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-11-01T00:00:00Z"}}, {"id": "10.1016/j.jasrep.2019.03.017", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:16:38Z", "type": "Journal Article", "created": "2019-03-25", "title": "Chemical and mineralogical analyses on stones from Sagunto Castle (Spain)", "description": "For the first time, an archaeometric study was carried out on the carbonate rock ashlars of the Sagunto Castle. The studied site is one of the most important and best preserved Spanish archaeological and architectural monuments, characterized by different construction phases from the Roman period to Modern Ages. Forty samples collected from thirteen different structures of Sagunto Castle and two quarries, located in the Sagunto's hill were used for comparative purposes. The samples were analyzed by X-ray diffraction, X-ray fluorescence and inductively coupled plasma mass spectrometry to determine their mineralogical and elemental composition. The obtained data show similar chemical and mineralogical features between the rocks outcropping in the city quarries and some of those employed to build the structures, suggesting that rocks could have been used to build the structures from different periods along the centuries.", "keywords": ["Building stone; Chemistry; Fortress; Middle ages; Mineralogy; Remains; Roman period", "Building stone; Fortress; Remains; Chemistry; Mineralogy; Roman period; Middle ages", "archaeometrics; carbonate rock ashlars; X-ray fluorescence and inductively coupled plasma mass spectrometry", "01 natural sciences", "0104 chemical sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://iris.unica.it/bitstream/11584/298931.2/3/Ramaciotti%20et%20al%202018_1-s2.0-S2352409X18307454-main.pdf"}, {"href": "https://doi.org/10.1016/j.jasrep.2019.03.017"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Archaeological%20Science%3A%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jasrep.2019.03.017", "name": "item", "description": "10.1016/j.jasrep.2019.03.017", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jasrep.2019.03.017"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-01T00:00:00Z"}}, {"id": "10.1016/j.jbiotec.2023.07.008", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:16:38Z", "type": "Journal Article", "created": "2023-07-25", "title": "Fast and reliable method to estimate global DNA methylation in plants and fungi with high-pressure liquid chromatography (HPLC)-ultraviolet detection and even more sensitive one with HPLC-mass spectrometry", "description": "DNA (Deoxyribonucleic acid) methylation is one of the epigenetic modifications of DNA, acting as a bridge between genotype and phenotype. Thus, disruption of DNA methylation pattern has tremendous consequences for organism development. Current methods to determine DNA methylation suffer from methodological drawbacks like high requirement of DNA and poor reproducibility of chromatograms. Here we provide a fast and reliable method using high-pressure liquid chromatography (HPLC)-ultraviolet (UV) detector and even more sensitive one with HPLC- mass spectrometry (MS) and we test this method with various plant and fungal DNA isolates. We optimized the preparation of the DNA degradation step to decrease background noise, we improved separation conditions to provide reliable and reproducible chromatograms and conditions to measure nucleotides in HPLC-MS. We showed that global DNA methylation level can be accurately and reproducibly measured with as little as 0.2\u00a0\u00b5M for HPLC-UV and 0.02\u00a0\u00b5M for HPLC-MS of methylated cytosine.", "keywords": ["Chromatography", "Plant DNA", "DNA methylation", "ta1183", "ta1182", "Fungi", "610", "Reproducibility of Results", "DNA Methylation", "Mass Spectrometry", "Fungal DNA", "chromatography", "DNA", " Fungal", "ta116", "Chromatography", " High Pressure Liquid"]}, "links": [{"href": "https://doi.org/10.1016/j.jbiotec.2023.07.008"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jbiotec.2023.07.008", "name": "item", "description": "10.1016/j.jbiotec.2023.07.008", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jbiotec.2023.07.008"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-09-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2023.109121", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:17:08Z", "type": "Journal Article", "created": "2023-07-04", "title": "When dry soil is re-wet, trehalose is respired instead of supporting microbial growth", "description": "When dry soil is re-wet there is a rapid increase in CO2 efflux and rates can remain above those of well-watered controls for one or more days. These large pulses of CO2 efflux are known as the `Birch effect. To provide experimental evidence of different pools of C fuelling the Birch effect, we incubated a drying soil with 13C6glucose, re wet the soil and quantified 13C labelling of pools (microbial biomass, trehalose, extracellular, and old C) and soil CO2 efflux. We took advantage of trehalose being the most 13C-enriched pool (&amp; delta;13C = +518%o) to obtain direct isotopic evidence of trehalose's contribution to respiration and microbial growth. For soil incubated with 13C6-glucose, the &amp; delta;13C of soil respiration was +35%o in dry soil, increased to 100%o in the 10 min following rewetting, and subsequently decreased. During the first 5 h after re-wetting, trehalose must have been contributing to respiration given that &amp; delta;13C of soil respiration was more 13C enriched than trehalose-free microbial biomass (&amp; delta;13C = +30%o), extracellular C (&amp; delta;13C = -17.7%o), and old C (&amp; delta;13C = -22.9%o). A four-member isotopic mixing model suggested trehalose underpinned 16% of respiration in the 1st hour after rewetting, decreasing to 7% in the fifth hour. At times beyond 5 h after rewetting, trehalose underpinned 0-4% of respiration. In the seven days following rewetting, microbial biomass increased 2292 nmol C g-1. Isotopic mass balance indicated trehalose-C could account for no more than 5% of the gross influx of C into microbial biomass, instead the increase in microbial biomass was fuelled by unlabelled or weakly labelled pools such as old C and extracellular C. Collectively these data provide direct experimental evidence C from trehalose does not significantly contribute to microbial growth in re-wet soil, but instead contributes to respiration for the first 5 h after rewetting.", "keywords": ["2. Zero hunger", "Ekologi", "Mass spectrometry", "Ecology", "Isotope", "Osmolyte", "Trehalose", "Jordbruk", " skogsbruk och fiske", "Birch effect", "Soil respiration", "Agriculture", " Forestry and Fisheries", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2023.109121"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2023.109121", "name": "item", "description": "10.1016/j.soilbio.2023.109121", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2023.109121"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-09-01T00:00:00Z"}}, {"id": "10.1016/j.trac.2019.04.008", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:17:20Z", "type": "Journal Article", "created": "2019-04-12", "title": "NORMAN digital sample freezing platform: A European virtual platform to exchange liquid chromatography high resolution-mass spectrometry data and screen suspects in \u201cdigitally frozen\u201d environmental samples", "description": "Abstract   A platform for archiving liquid chromatography high-resolution mass spectrometry (LC-HRMS) data was developed for the retrospective suspect screening of thousands of environmental pollutants with the ambition of becoming a European and possibly global standard. It was termed Digital Sample Freezing Platform (DSFP) and incorporates all the recent developments in the HRMS screening methods within the NORMAN Network. In the workflow, raw mass spectral data are converted into mzML, then mass spectral and chromatographic information on thousands of peaks of each sample is extracted into Data Collection Templates. The \u2018digitally frozen\u2019 samples can be retrospectively screened for the presence of virtually any compound amenable to LC\u2013MS using a combination of information on its (i) exact mass, (ii) predicted retention time window in the chromatogram, (iii) isotopic fit and (iv) qualifier fragment ions. Its potential was demonstrated on monitoring of 670 antibiotics and 777 REACH chemicals from the Joint Black Sea Surveys (JBSS).", "keywords": ["Digital sample freezing platform High resolution mass spectrometry Digital archiving Suspect screening Antibiotics REACH Black sea"]}, "links": [{"href": "https://doi.org/10.1016/j.trac.2019.04.008"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/TrAC%20Trends%20in%20Analytical%20Chemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.trac.2019.04.008", "name": "item", "description": "10.1016/j.trac.2019.04.008", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.trac.2019.04.008"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-01T00:00:00Z"}}, {"id": "10.1016/j.trac.2022.116819", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:17:20Z", "type": "Journal Article", "created": "2022-11-11", "title": "Microplastics and nanoplastics in food, water, and beverages, part II. Methods", "description": "Since microplastics (MPs) and nanoplastics (NPs) have started emerging as ubiquitous contaminants in the environment, a variety of analytical techniques has been developed and tested for the detection and characterization of polymer particles at a micro- and nano-scale. Yet, no unique method stands out for its ability to yield all the relevant information required to properly address MP and NP contamination in the environment, and even less so in food material. In this review, several approaches to sample preparation and isolation of MPs from food matrices are reported, well-established and promising emerging analytical techniques for the detection and characterization of MPs and NPs are described and discussed. The information reported in this review shows that even the most widely used methods are still under development and MP/NP analysis is still far away from method validation and standardization. The establishment of rigorous best practices to yield reliable data and build a comprehensive knowledge of MP and NP occurrence in food is essential for the implementation of strategies and policies to address MP/NP pollution. Therefore, an outlook of the field towards harmonization and quality improvement of MP/NP analysis is included in this review.", "keywords": ["Vibrational spectroscopy", "Microscopy", "Mass spectrometry", "Sample preparation", "0211 other engineering and technologies", "02 engineering and technology", "01 natural sciences", "6. Clean water", "12. Responsible consumption", "FTIR", "13. Climate action", "Validation", "AFM-IR", "Raman", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.trac.2022.116819"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/TrAC%20Trends%20in%20Analytical%20Chemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.trac.2022.116819", "name": "item", "description": "10.1016/j.trac.2022.116819", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.trac.2022.116819"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-01T00:00:00Z"}}, {"id": "10.1021/acs.analchem.2c04345", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:17:25Z", "type": "Journal Article", "created": "2022-12-22", "title": "Rapid Single Particle Atmospheric Solids Analysis Probe-Mass Spectrometry for Multimodal Analysis of Microplastics", "description": "Despite mass spectrometry (MS) being proven powerful for the characterization of synthetic polymers, its potential for the analysis of single particle microplastics (MPs) is yet to be fully disclosed. To date, MPs are regarded as ubiquitous contaminants, but the limited availability of techniques that enable full characterizations of MPs results in a lack of systematic data regarding their occurrence. In this study, an atmospheric solid analysis probe (ASAP) coupled to a compact quadrupole MS is proposed for the chemical analysis of single particle microplastics, while maintaining full compatibility with complementary staining and image analysis approaches. A two-stage ASAP probe temperature program was optimized for the removal of additives and surface contaminants followed by the actual polymer characterization. The method showed specific mass spectra for a wide range of single particle MPs, including polyolefins, polyaromatics, polyacrylates, (bio)polyesters, polyamides, polycarbonates, and polyacrylonitriles. The single particle size detection limits for polystyrene MPs were found to be 30 and 5 \u03bcm in full scan and selected ion recording mode, respectively. Moreover, results are presented of a multimodal microplastic analysis approach in which filtered particles are first characterized by staining and fluorescence microscopy, followed by simple probe picking of individual particles for subsequent analysis by ASAP-MS. The method provides a full characterization of MP contamination, including particle number, particle size, particle shape, and chemical identity. The applicability of the developed multimodal method was successfully demonstrated by the analysis of MPs in bioplastic bottled water.", "keywords": ["Microplastics", "Life Science", "Plastics", "01 natural sciences", "Gas Chromatography-Mass Spectrometry", "Water Pollutants", " Chemical", "Mass Spectrometry", "Environmental Monitoring", "0104 chemical sciences"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.2c04345"}, {"href": "https://doi.org/10.1021/acs.analchem.2c04345"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Analytical%20Chemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.analchem.2c04345", "name": "item", "description": "10.1021/acs.analchem.2c04345", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.analchem.2c04345"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-12-22T00:00:00Z"}}, {"id": "10.1021/acs.est.0c08208", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:17:26Z", "type": "Journal Article", "created": "2021-05-19", "title": "An Automated Methodology for Non-targeted Compositional Analysis of Small Molecules in High Complexity Environmental Matrices Using Coupled Ultra Performance Liquid Chromatography Orbitrap Mass Spectrometry", "description": "<strong>Abstract</strong> The life-critical matrices of air and water are among the most complex chemical mixtures that are ever encountered. Ultra-high resolution mass spectrometers, such as the Orbitrap, provide unprecedented analytical capabilities to probe the molecular composition of such matrices, but the extraction of non-targeted chemical information is impractical to perform <em>via</em> manual data processing. Automated non-targeted tools rapidly extract the chemical information of all detected compounds within a sample dataset. However, these methods have not been exploited in the environmental sciences. Here, we provide an automated and (for the first time) rigorously tested methodology for the non-targeted compositional analysis of environmental matrices using coupled liquid chromatography-mass spectrometric data. First, the robustness and reproducibility was tested using authentic standards, evaluating performance as a function of concentration, ionization potential and sample complexity. The method was then used for the compositional analysis of particulate matter and surface waters collected from world-wide locations. The method detected &gt;9,600 compounds in the individual environmental samples, arising from critical pollutant sources, including carcinogenic industrial chemicals, pesticides, pharmaceuticals,<em> </em>among others. This methodology offers considerable advances in the environmental sciences, providing a more complete assessment of sample compositions, whilst significantly increasing throughput.", "keywords": ["13. Climate action", "1600", "2304", "Reproducibility of Results", "Pesticides", "01 natural sciences", "Chromatography", " High Pressure Liquid", "Mass Spectrometry", "Water Pollutants", " Chemical", "Chromatography", " Liquid", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://eprints.bournemouth.ac.uk/36790/1/An%20Automated%20Methodology%20for%20Non-targeted%20Compositional%20Analysis%20of%20Small%20Molecules%20in%20High%20Complexity%20Environmental%20Matrice.pdf"}, {"href": "https://eprints.whiterose.ac.uk/174399/1/acs.est.0c08208.pdf"}, {"href": "https://doi.org/10.1021/acs.est.0c08208"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.est.0c08208", "name": "item", "description": "10.1021/acs.est.0c08208", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.est.0c08208"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-05-18T00:00:00Z"}}, {"id": "10.1021/acsmeasuresciau.3c00027", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:17:28Z", "type": "Journal Article", "created": "2023-09-08", "title": "Evaluation of Pure PFAS Decrease in Controlled Settings", "description": "Since 1940, poly- or perfluorinated alkyl substances (PFAS) have been largely used in many applications, including paints, fire foaming, household items, product packaging, and fabrics. Because of their extremely high persistency, they have been defined as 'forever chemicals'. Although the EU is taking action to reduce their use, their widespread occurrence in environmental matrices and their harmful effects on human health require the use of highly performing analytical methods for efficient monitoring. Furthermore, novel PFAS are constantly revealed by both EU and National environmental agencies. The objective of this work is to investigate the cause of the signal decrease during the analysis of a standard PFAS mixture in water-based matrices, by proposing an efficient technical procedure for laboratory specialists. The analyses were carried out on a mixture of 30 PFAS, including both regulated and unknown substances (which are expected to be introduced in the guidelines), characterized by different chemical features, using LC-vials of two different materials, namely, glass and polypropylene, and dissolved in two solvents, namely, water and water-methanol. The temperature of analysis and the concentration of PFAS were also considered through LC-MS analyses at different times, in the 0-15 h range. Depending on the chemical structure and length of the PFAS, sampling and treatment procedures may be adopted to tackle the decrease and the release from the containers, reducing the risk of underestimating PFAS also in real water matrices.", "keywords": ["pfas; mass spectrometry; adsorption; drinking water; containers", "QD71-142", "Analytical chemistry"]}, "links": [{"href": "https://iris.uniroma1.it/bitstream/11573/1696744/1/Mancini_Evaluation_2023.pdf"}, {"href": "https://pubs.acs.org/doi/pdf/10.1021/acsmeasuresciau.3c00027"}, {"href": "https://doi.org/10.1021/acsmeasuresciau.3c00027"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ACS%20Measurement%20Science%20Au", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acsmeasuresciau.3c00027", "name": "item", "description": "10.1021/acsmeasuresciau.3c00027", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acsmeasuresciau.3c00027"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-09-07T00:00:00Z"}}, {"id": "10.1021/acs.jafc.1c02821", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:17:27Z", "type": "Journal Article", "created": "2021-08-04", "title": "Determination of the Effect of Co-cultivation on the Production and Root Exudation of Flavonoids in Four Legume Species Using LC\u2013MS/MS Analysis", "description": "Flavonoids play a key role in the regulation of plant-plant and plant-microbe interactions, and factors determining their release have been investigated in most of the common forage legumes. However, little is known about the response of flavonoid production and release to co-cultivation with other crop species. This study investigated alterations in the concentration of flavonoids in plant tissues and root exudates in four legumes [alfalfa (Medicago sativa L.), black medic (Medicago polymorpha L.), crimson clover (Trifolium incarnatum L.), and subterranean clover (Trifolium subterraneum L.)] co-cultivated with durum wheat [Triticum turgidum subsp. durum (Desf.) Husn.]. For this purpose, we carried out two experiments in a greenhouse, one with glass beads as growth media for root exudate extraction and one with soil as growth media for flavonoid detection in shoot and root biomass, using LC-MS/MS analysis. This study revealed that interspecific competition with wheat negatively affected legume growth and led to a significant reduction in shoot and root biomass compared with the same legume species grown in monoculture. In contrast, the concentration of flavonoids significantly increased both in legume biomass and in root exudates. Changes in flavonoid concentration involved daidzein, genistein, medicarpin, and formononetin, which have been found to be involved in legume nodulation and regulation of plant-plant interaction. We hypothesize that legumes responded to the co-cultivation with wheat by promoting nodulation and increasing exudation of allelopathic compounds, respectively, to compensate for the lack of nutrients caused by the presence of wheat in the cultivation system and to reduce the competitiveness of neighboring plants. Future studies should elucidate the bioactivity of flavonoid compounds in cereal-legume co-cultivation systems and their specific role in the nodulation process and inter-specific plant interactions such as potential effects on weeds.", "keywords": ["Flavonoids", "2. Zero hunger", "0301 basic medicine", "0303 health sciences", "medicarpin", "living mulch", "root exudate", "Fabaceae", "15. Life on land", "plant interactions", "Plant Roots", "03 medical and health sciences", "Tandem Mass Spectrometry", "wheat", "allelopathy", "intercropping", "wheat", " intercropping", " living mulch", " root exudate", " plant interactions", " medicarpin", " allelopathy", "Chromatography", " Liquid"]}, "links": [{"href": "https://www.iris.sssup.it/bitstream/11382/540035/1/Leoni%20et%20al_2021_jafc_1c02821.pdf"}, {"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.1c02821"}, {"href": "https://doi.org/10.1021/acs.jafc.1c02821"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Agricultural%20and%20Food%20Chemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.jafc.1c02821", "name": "item", "description": "10.1021/acs.jafc.1c02821", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.jafc.1c02821"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-08-04T00:00:00Z"}}, {"id": "10.1021/acs.jafc.3c04532", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:17:27Z", "type": "Journal Article", "created": "2023-10-05", "title": "Ultra-high Performance Liquid Chromatography\u2013Ion Mobility\u2013High-Resolution Mass Spectrometry to Evaluate the Metabolomic Response of Durum Wheat to Sustainable Treatments", "description": "Sustainable agriculture aims at achieving a healthy food production while reducing the use of fertilizers and greenhouse gas emissions using biostimulants and soil amendments. Untargeted metabolomics by ultra-high performance liquid chromatography-ion mobility-high-resolution mass spectrometry, operating in a high-definition MSE mode, was applied to investigate the metabolome of durum wheat in response to sustainable treatments, i.e., the addition of biochar, commercial plant growth promoting microbes, and their combination. Partial least squares-discriminant analysis provided a good discrimination among treatments with sensitivity, specificity, and a non-error rate close to 1. A total of 88 and 45 discriminant compounds having biological, nutritional, and technological implications were tentatively identified in samples grown in 2020 and 2021. The addition of biochar-biostimulants produced the highest up-regulation of lipids and flavonoids, with the glycolipid desaturation being the most impacted pathway, whereas carbohydrates were mostly down-regulated. The findings achieved suggest the safe use of the combined biochar-biostimulant treatment for sustainable wheat cultivation.", "keywords": ["2. Zero hunger", "Settore CHEM-01/A - Chimica analitica", "630", "Mass Spectrometry", "12. Responsible consumption", "ultra-high performance liquid chromatography\u2212high-resolution mass spectrometry ion mobility untargeted metabolomics multivariate data analysis durum wheat biostimulants soil amendments", "13. Climate action", "Settore AGRI-06/A - Genetica agraria", "615", "Metabolomics", "ultra-high performance liquid chromatography\u2013high-resolution mass spectrometry ion mobility untargeted metabolomics multivariate data analysis durum wheat biostimulants soil amendments", "Settore BIOS-10/A - Biologia cellulare e applicata", "Chromatography", " High Pressure Liquid", "Triticum"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.3c04532"}, {"href": "https://doi.org/10.1021/acs.jafc.3c04532"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Agricultural%20and%20Food%20Chemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.jafc.3c04532", "name": "item", "description": "10.1021/acs.jafc.3c04532", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.jafc.3c04532"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-05T00:00:00Z"}}, {"id": "10.1021/jf302931z", "type": "Feature", "geometry": null, "properties": {"license": "unspecified", "updated": "2026-07-28T16:17:29Z", "type": "Journal Article", "created": "2012-10-18", "title": "Fertilizing Soil With Selenium Fertilizers: Impact On Concentration, Speciation, And Bioaccessibility Of Selenium In Leek (Allium Ampeloprasum)", "description": "Leek was fertilized with sodium selenite (Na(2)SeO(3)) and sodium selenate (Na(2)SeO(4)) in a green house to assess the impact of selenium (Se) fertilization on Se uptake by the crop and its speciation in the crop. The bioaccessibility of Se in the Se-enriched leek was assessed using an in vitro extraction protocol mimicking the human gastrointestinal tract (stomach, small intestine, and colon). The lowest Se uptake was observed when Na(2)SeO(3) was used as a fertilizer, which results in a higher risk for Se accumulation in the soil on a longer term. When soil was amended with Na(2)SeO(4), 55 \u00b1 5% of total Se in the leek occurred in an inorganic form, while only 21 \u00b1 8% was inorganic when Na(2)SeO(3) was applied. Se-methylselenocysteine and selenomethione were the major organic species in both treatments. However, concentrations of Se-methylselenocysteine and \u03b3-glutamyl-Se-methyl-selenocysteine, which were previously reported to induce positive health effects, were lower as compared to other Allium species. The majority of the Se in the leek was found to be bioaccessible in the stomach (around 60%) and small intestine (around 80%). However, a significant fraction also has good chances to reach the colon, where it seems to be taken up by the microbial community and may also induce positive health effects.", "keywords": ["2. Zero hunger", "Selenium", "Soil", "Onions", "Biological Availability", "15. Life on land", "Fertilizers", "01 natural sciences", "Mass Spectrometry", "6. Clean water", "0104 chemical sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1021/jf302931z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Agricultural%20and%20Food%20Chemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/jf302931z", "name": "item", "description": "10.1021/jf302931z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/jf302931z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-10-30T00:00:00Z"}}, {"id": "10.1039/d2np00011c", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:17:52Z", "type": "Journal Article", "created": "2022-06-23", "title": "Image to insight: exploring natural products through mass spectrometry imaging", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>This review provides an up-to-date summary of mass spectrometry imaging, emphasizing different applications and recent innovations in natural product research.</p></article>", "keywords": ["0301 basic medicine", "Biological Products", "0303 health sciences", "03 medical and health sciences", "Plants", "Mass Spectrometry", "Molecular Imaging"]}, "links": [{"href": "http://pubs.rsc.org/en/content/articlepdf/2022/NP/D2NP00011C"}, {"href": "https://doi.org/10.1039/d2np00011c"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Natural%20Product%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1039/d2np00011c", "name": "item", "description": "10.1039/d2np00011c", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1039/d2np00011c"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}, {"id": "10.1111/nph.19450", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:19:01Z", "type": "Journal Article", "created": "2023-11-30", "title": "A step forward in fungal biomass estimation \u2013 a new protocol for more precise measurements of soil ergosterol with liquid chromatography\u2010mass spectrometry and comparison of extraction methods", "description": "Significant differences between ergosterol protocols and their yield call for harmonization of methodologies. We propose an extraction protocol with KOH in methanol with cyclohexane for liquid\u2013liquid extraction. The novel LC\u2013MS method proved to be superior to the HPLC-UV method for soil samples because of the higher quality of the peaks and the possibility of following peak purity. Moreover, the LC\u2013MS method has 10 times lower detection limit than that of HPLC-UV.", "keywords": ["Soil", "selected ion recording", "Liquid Chromatography-Mass Spectrometry", "Ergosterol", "mineral soil", "ta1182", "Fungi", "ergosterol fragmentation", "Biomass", "540", "forest soil", "peatlands", "Soil Microbiology"]}, "links": [{"href": "https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.19450"}, {"href": "https://doi.org/10.1111/nph.19450"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Phytologist", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/nph.19450", "name": "item", "description": "10.1111/nph.19450", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/nph.19450"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-11-29T00:00:00Z"}}, {"id": "10.1139/as-2022-0006", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:19:07Z", "type": "Journal Article", "created": "2022-07-04", "title": "Monitoring guidelines for polymer identification, quality assurance/quality control (QA/QC) and data reporting for monitoring of microplastics in the Arctic environment", "description": "<p> The pollution of the environment with plastics is of growing concern worldwide, including the Arctic region. While larger plastic pieces are a visible pollution issue, smaller microplastics are not visible with the naked eye. These particles are available for interaction by Arctic biota and have become a concern for animal and human health. The determination of microplastic properties includes several methodological steps, i.e., sampling, extraction, quantification, and chemical identification. This review discusses suitable analytical tools for the identification, quantification, and characterization of microplastics in the context of monitoring in the Arctic. It further addresses quality assurance and quality control (QA/QC), which is particularly important for the determination of microplastic in the Arctic, as both contamination and analyte losses can occur. It presents specific QA/QC measures for sampling procedures and for the handling of samples in the laboratory, either on land or on ship, and considering the small size of microplastics as well as the high risk of contamination. The review depicts which data should be mandatory to report, thereby supporting a framework for harmonized data reporting. </p>", "keywords": [":Analytisk kjemi: 445 [VDP]", "0211 other engineering and technologies", "Environmental engineering", "QA/QC", "02 engineering and technology", "Massespektrografi", "01 natural sciences", "[SDU] Sciences of the Universe [physics]", ":Analytical chemistry: 445 [VDP]", "Arctic", "VDP::Analytical chemistry: 445", "GE1-350", "14. Life underwater", "QA", "Raman", "QC", "0105 earth and related environmental sciences", "reporting", "Mass spectrometry", "TED-GC/MS", "TED-GC", "py-GC/MS", "Microplastic", "py-GC", "Fourier transform infrared spectroscopy", "MS", "VDP::Analytisk kjemi: 445", "TA170-171", "Microplast", "620", "Environmental sciences", "[SDV] Life Sciences [q-bio]", "monitoring", "FTIR", "13. Climate action", "microscopy", "microplastic"]}, "links": [{"href": "https://iris.cnr.it/bitstream/20.500.14243/536963/1/primpke-et-al-2022-monitoring-of-microplastic-pollution-in-the-arctic-recent-developments-in-polymer-identification.pdf"}, {"href": "https://cdnsciencepub.com/doi/pdf/10.1139/as-2022-0006"}, {"href": "https://doi.org/10.1139/as-2022-0006"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Arctic%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1139/as-2022-0006", "name": "item", "description": "10.1139/as-2022-0006", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1139/as-2022-0006"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-03-01T00:00:00Z"}}, {"id": "10.1371/journal.pone.0096182", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:19:22Z", "type": "Journal Article", "created": "2014-05-09", "title": "Biotic And Abiotic Properties Mediating Plant Diversity Effects On Soil Microbial Communities In An Experimental Grassland", "description": "Plant diversity drives changes in the soil microbial community which may result in alterations in ecosystem functions. However, the governing factors between the composition of soil microbial communities and plant diversity are not well understood. We investigated the impact of plant diversity (plant species richness and functional group richness) and plant functional group identity on soil microbial biomass and soil microbial community structure in experimental grassland ecosystems. Total microbial biomass and community structure were determined by phospholipid fatty acid (PLFA) analysis. The diversity gradient covered 1, 2, 4, 8, 16 and 60 plant species and 1, 2, 3 and 4 plant functional groups (grasses, legumes, small herbs and tall herbs). In May 2007, soil samples were taken from experimental plots and from nearby fields and meadows. Beside soil texture, plant species richness was the main driver of soil microbial biomass. Structural equation modeling revealed that the positive plant diversity effect was mainly mediated by higher leaf area index resulting in higher soil moisture in the top soil layer. The fungal-to-bacterial biomass ratio was positively affected by plant functional group richness and negatively by the presence of legumes. Bacteria were more closely related to abiotic differences caused by plant diversity, while fungi were more affected by plant-derived organic matter inputs. We found diverse plant communities promoted faster transition of soil microbial communities typical for arable land towards grassland communities. Although some mechanisms underlying the plant diversity effect on soil microorganisms could be identified, future studies have to determine plant traits shaping soil microbial community structure. We suspect differences in root traits among different plant communities, such as root turnover rates and chemical composition of root exudates, to structure soil microbial communities.", "keywords": ["0301 basic medicine", "570", "Chromatography", " Gas", "Geography & travel", "Science", "910", "Plant Roots", "Gas Chromatography-Mass Spectrometry", "Soil", "03 medical and health sciences", "info:eu-repo/classification/ddc/910", "Biomass", "Phospholipids", "Soil Microbiology", "2. Zero hunger", "0303 health sciences", "Bacteria", "Q", "Fatty Acids", "R", "Fungi", "Water", "Biodiversity", "Models", " Theoretical", "Plants", "15. Life on land", "ddc:910", "Grassland", "13. Climate action", "Medicine", "Research Article"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0096182"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0096182", "name": "item", "description": "10.1371/journal.pone.0096182", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0096182"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-05-09T00:00:00Z"}}, {"id": "10261/377128", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:23:51Z", "type": "Journal Article", "created": "2021-08-07", "title": "Groundwater antibiotic pollution and its relationship with dissolved organic matter: Identification and environmental implications", "description": "The occurrence of veterinary antibiotics and hydro-chemical parameters in eleven natural springs in a livestock production area is evaluated, jointly with the characterization of their DOM fingerprint by Orbitrap HRMS. Tetracycline and sulfonamide antibiotics were ubiquitous in all sites, and they were detected at low ng L-1 concentrations, except for doxycycline, that was present at \u03bcg L-1 in one location. DOM analysis revealed that most molecular formulas were CHO compounds (49 %-68\u00a0%), with a remarkable percentage containing nitrogen and sulphur (16 %-23\u00a0% and 11 %-24\u00a0%, respectively). Major DOM components were phenolic and highly unsaturated compounds (~90\u00a0%), typical for soil-derived organic matter, while approximately 11\u00a0% were unsaturated aliphatic, suggesting that springs may be susceptible to anthropogenic contamination sources. Comparing the DOM fingerprint among sites, the spring showing the most different profile was the one with surface water interaction and characterized by having lower CHO and higher CHOS formulas and aliphatic compounds. Correlations between antibiotics and DOM showed that tetracyclines positively correlate with unsaturated oxygen-rich substances, while sulfonamides relate with aliphatic and unsaturated oxygen-poor compounds. This indicates that the fate of different antibiotics will be controlled by the type of DOM present in groundwater.", "keywords": ["High-resolution mass spectrometry", "550", "Contaminants emergents en l'aigua", "Antibi\u00f2tics", "02 engineering and technology", "01 natural sciences", "630", "Soil", "Antibiotics", "Co-transport", "Groundwater -- Pollution", "Dissolved organic matter", "Groundwater", "0105 earth and related environmental sciences", "2. Zero hunger", "[SDU.OCEAN]Sciences of the Universe [physics]/Ocean", "Emerging contaminants in water", "Atmosphere", "[SDU.OCEAN] Sciences of the Universe [physics]/Ocean", " Atmosphere", "[SDU.ENVI] Sciences of the Universe [physics]/Continental interfaces", " environment", "6. Clean water", "Anti-Bacterial Agents", "Water quality", "13. Climate action", "Aig\u00fces subterr\u00e0nies -- Contaminaci\u00f3", "[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces", "0210 nano-technology", "environment", "Water Pollutants", " Chemical", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10261/377128"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10261/377128", "name": "item", "description": "10261/377128", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10261/377128"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-11-01T00:00:00Z"}}, {"id": "10.1594/pangaea.960025", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:19:37Z", "type": "Dataset", "title": "Analysis of the high density coarse fraction (HDC) from the nearshore zone of Herschel Island (Yukon, Canada)", "keywords": ["14C AMS", "MICADAS accelerator mass spectrometer AMS", "Elemental analyzer (EA)", " Thermo Scientific", " FlashEA 1112", "Fraction modern carbon", "Permafrost", "Quantachrome", "Age", " dated", "Surface area analyzer", " Quantachrome", " Nova 4200e; 6-point Brunauer\u2013Emmett\u2013Teller (BET) method according to Brunauer et al. (1938)", "Arctic", "Elemental analyzer EA", "Isotope ratio mass spectrometry-elemental analyzer (IRMS-EA)", " Thermo Finnigan", " Delta XP; Elemental analyzer (EA)", " Thermo Scientific", " FlashEA 1112", "Thermo Scientific", "Calculated", "Carbon Nitrogen ratio", "Carbon", " organic", " loading", "total", "Laboratory code label", "Minerals", "Multiple investigations", "Laboratory code/label", "Ionplus according to McIntyre et al 2017 and Haghipour et al 2018", "Minerals", " surface area", "error", "loading", "Nitrogen", " total", "Earth System Research", "\u03b413C", "FlashEA 1112", "Isotope ratio mass spectrometry elemental analyzer IRMS EA", "Nitrogen", "organic", "\u039414C", "MICADAS accelerator mass spectrometer (AMS)", " Ionplus; according to McIntyre et al. (2017) and Haghipour et al. (2018)", "Surface area analyzer", "dated", "Age", "Delta XP Elemental analyzer EA", "Organic carbon", "Comment", "surface area", "Carbon", "Carbon/Nitrogen ratio", "relative", "Thermo Finnigan", "sediment", "Sample ID", "Age", " 14C AMS", "Biomarkers", "Nova 4200e 6 point Brunauer Emmett Teller BET method according to Brunauer et al 1938", "Carbon", " organic", " total", "Fraction modern carbon", " error", " relative"]}, "links": [{"href": "https://doi.org/10.1594/pangaea.960025"}, {"rel": "self", "type": "application/geo+json", "title": "10.1594/pangaea.960025", "name": "item", "description": "10.1594/pangaea.960025", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1594/pangaea.960025"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "10.1594/pangaea.963212", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:19:37Z", "type": "Dataset", "title": "Stream water chemistry and landscape characteristics in Zackenberg Valley, NE Greenland summer 2021", "description": "The data contains water chemistry and spectral catchment NDVI for 14 streams in Zackenberg Valley in Northeast Greenland, sampled summer 2021 from 10th July to 15th September. We collected water samples for measuring water chemistry, and we determined landscape parameters using GIS based tools. The data was collected at three sampling periods in summer 2021 in the Zackenberg Research Station (74\u00b028'N, 20\u00b034'W). The area has a polar tundra climate with mean annual air temperature of -9.1 \u00b0C. Water chemistry (i.e. dissolved and particulate nitrogen, phosphorus, carbon; dissolved iron and silicate) and catchment characteristics (i.e. catchment area, altitude, slope, aspect, NDVI, snow cover) was measured for each of the 14 stream sites. Water chemistry samples were collected and analyzed using standard methods, and landscape characteristics were determined using GIS resources. The data was collected in order to study relationships between landscape characteristics and stream water chemistry. The water samples were collected by a team of two people, and the detailed methods are given below.", "keywords": ["inorganic", "median", "Nitrate Nitrogen", "Nitrogen", " inorganic", " dissolved/Nitrogen", " total dissolved ratio", "Nitrate", "Normalized Difference Vegetation Index", "Latitude of event", "Inductively Coupled Plasma Mass Spectrometry ICP MS", "Arctic", "Temperature", " water", "WTW", "Total organic carbon analyzer TOC VCPH TNM 1", "Total organic carbon analyzer (TOC-VCPH/TNM-1)", " Shimadzu", "Calculated", "dissolved ratio", "Nitrate/Nitrogen", " inorganic", " dissolved ratio", "total dissolved ratio", "Multiple investigations", "Temperature", "Nitrogen", " total dissolved", "Month", "dissolved", "specific", "streams", "6. Clean water", "Nitrogen", " inorganic", " dissolved", "Chemistry", "Inductively Coupled Plasma - Mass Spectrometry (ICP-MS)", " PerkinElmer Instruments", " Optima 2000 DV", "Sum cations", "Natural Sciences", "Ammonium", "Potassium Silicon ratio", "Calcium Magnesium ratio", "Conductivity Meter", " WTW", " ProfiLine Cond 3110", "Longitude of event", "Silicon", "Lachat QuickChem 8500 flow injection autoanalyser", "Nitrogen", "organic", "water chemistry", "Iron", "Calcium/Magnesium ratio", "water", "Site", "Nitrate/Ammonium ratio", "Aspect", "Normalized Differenced Vegetation Index", " median", "Ammonium Nitrogen", "Normalized Differenced Vegetation Index", "Catchment area", "Slope", "PerkinElmer Instruments", "ProfiLine Cond 3110", "Shimadzu", "Date/Time of event", "Conductivity Meter", "Nitrate Ammonium ratio", "total dissolved", "Conductivity", "Event label", "Date Time of event", "Nitrogen", " inorganic", " dissolved/Nitrogen", " organic", " dissolved ratio", "15. Life on land", "Carbon", " organic", " dissolved", "dissolved Nitrogen", "Elevation of event", "Carbon", "rivers", "Snow coverage", "Greening", "Potassium/Silicon ratio", "Optima 2000 DV", "Nitrogen", " organic", " dissolved", "13. Climate action", "Discharge", "Conductivity", " specific", "Ammonium/Nitrogen", " inorganic", " dissolved ratio"], "contacts": [{"organization": "Riis, Tenna, Tank, Jennifer, Holmboe, Cecilie Marie Hartvig, Gim\u00e9nez-Grau, Pau, Mastepanov, Mikhail, Catalan, Nuria, Stott, David, Hansen, Birgitte, Kristiansen, S\u00f8ren M, Pastor, Ada,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1594/pangaea.963212"}, {"rel": "self", "type": "application/geo+json", "title": "10.1594/pangaea.963212", "name": "item", "description": "10.1594/pangaea.963212", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1594/pangaea.963212"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-01T00:00:00Z"}}, {"id": "10.1594/pangaea.972412", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:19:38Z", "type": "Dataset", "title": "Radiocarbon content and age of terrestrial material deposited at the paleo-delta of the Lena River at the transition to the Preboreal", "description": "The dataset was used to reconstruct the release of permafrost organic carbon from the watershed of the Lena River (Russia) between 11.1 and 11.7 calibrated thousand years Before Present (cal. kyr BP, Present = 1950 AD) and to model potential methane emissions from this carbon source. Data were obtained analyzing plant debris isolated from the low density fraction (&lt;1.8 g/cm3) of muddy sediments from the Piston Core 23 (PC23). The sediment core was retrieved in July 2014 in the mid/outer-shelf of the Laptev Sea shelf (76\u00b0 10' 15.6''N; 129\u00b0 20' 13.2''E, water depth of 56 m) during Leg 1 of the SWERUS-C3 expedition (Swedish-Russian-U.S. Arctic Ocean \u2013 Investigation of Climate-Cryosphere-Carbon interactions). Radiocarbon (\u00b9\u2074C) content measurements were performed on 4 to 7 mg of sample on May 2022 using the Continuous Flow Accelerator Mass Spectrometry (CFAMS) system at the National Ocean Sciences Accelerator Mass Spectrometry (NOSAMS) facility (Woods Hole Oceanographic Institution, Woods Hole, MA, USA). The \u00b9\u2074C content was used to calculate \u00b9\u2074C isotope ratios (D\u00b9\u2074C) and to reconstruct non-calibrated and calibrated \u00b9\u2074C ages of plant debris isolated from the sediment fraction with a density lower than 1.8 g/cm3. Additionally, D\u00b9\u2074C values were used to calculate the \u00b9\u2074C content of plant debris at time of sediment deposition (D\u00b9\u2074Ci) and the latter to obtain pre-depositional \u00b9\u2074C ages. The dataset allowed to reconstruct the age of the terrestrial plant material when deposited at the coring site and identify its provenance from within the permafrost OC pool (sub-surface soil within 1 m depth vs deep soil) after remobilization. The average pre-depositional \u00b9\u2074C age of plant debris was ultimately used to model methane emissions from relatively young permafrost organic carbon during the late deglaciation (ca. 10 to 15 cal. kyr BP).", "keywords": ["Piston corer", "SWERUS C3", "Longitude of event", "Age", " 14C", "\u039414C", "Fraction modern carbon", "Calendar age", "Permafrost", "DEPTH", " sediment/rock", "Latitude of event", "Oden", "Age", "Arctic", "AGE", "pre depositional", "bottom maximum", "top min", "Date/Time of event", "Calculated", "\u039414C", " initial", " error", "Fraction modern carbon", " error", "Sample code/label", "Deglaciation", "Depth", " sediment/rock", " bottom/maximum", "\u039414C", " error", "Age", " pre-depositional", " error", "Depth", "Event label", "Date Time of event", "Continuous Flow Accelerator Mass Spectrometry", "initial", "Sample code label", "Depth", " top/min", "Calendar age", " standard error", "Age", " error", "error", "Elevation of event", "sediment rock", "\u039414C", " initial", "14C", "standard error", "DEPTH", "Age", " pre-depositional", "Earth System Research", "SWERUS-C3", "Methane"], "contacts": [{"organization": "Sabino, Mathia, Gustafsson, \u00d6rjan, Wild, Birgit, Semiletov, Igor P, Dudarev, Oleg V, Ingrosso, Gianmarco, Tesi, Tommaso,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1594/pangaea.972412"}, {"rel": "self", "type": "application/geo+json", "title": "10.1594/pangaea.972412", "name": "item", "description": "10.1594/pangaea.972412", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1594/pangaea.972412"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-01T00:00:00Z"}}, {"id": "10.3390/ijms25168599", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:20:41Z", "type": "Journal Article", "created": "2024-08-07", "title": "Design of Novel Membranes for the Efficient Separation of Bee Alarm Pheromones in Portable Membrane Inlet Mass Spectrometric Systems", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Bee alarm pheromones are essential molecules that are present in beehives when some threats occur in the bee population. In this work, we have applied multilevel modeling techniques to understand molecular interactions between representative bee alarm pheromones and polymers such as polymethyl siloxane (PDMS), polyethylene glycol (PEG), and their blend. This study aimed to check how these interactions can be manipulated to enable efficient separation of bee alarm pheromones in portable membrane inlet mass spectrometric (MIMS) systems using new membranes. The study involved the application of powerful computational atomistic methods based on a combination of modern semiempirical (GFN2-xTB), first principles (DFT), and force-field calculations. As a fundamental work material for the separation of molecules, we considered the PDMS polymer, a well-known sorbent material known to be applicable for light polar molecules. To improve its applicability as a sorbent material for heavier polar molecules, we considered two main factors\u2014temperature and the addition of PEG polymer. Additional insights into molecular interactions were obtained by studying intrinsic reactive properties and noncovalent interactions between bee alarm pheromones and PDMS and PEG polymer chains.</p></article>", "keywords": ["0103 physical sciences", "Animals", "Membranes", " Artificial", "Dimethylpolysiloxanes", "Bees", "01 natural sciences", "Article", "Pheromones", "Mass Spectrometry", "Polyethylene Glycols", "0104 chemical sciences"]}, "links": [{"href": "https://doi.org/10.3390/ijms25168599"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Molecular%20Sciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/ijms25168599", "name": "item", "description": "10.3390/ijms25168599", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/ijms25168599"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-07T00:00:00Z"}}, {"id": "10.3390/metabo11070438", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:20:43Z", "type": "Journal Article", "created": "2021-07-05", "title": "Metabolomic Approaches to Studying the Response to Drought Stress in Corn (Zea mays) Cobs", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Metabolomics is a technique that allows for the evaluation of the entire extractable chemical profile of a plant, for example, using high-resolution mass spectrometry (HRMS) and can be used to evaluate plant stress responses, such as those due to drought. Metabolomic analysis is dependent upon the efficiency of the extraction protocol. Currently, there are two common extraction procedures widely used in metabolomic experiments, those that extract from plant tissue processed in liquid nitrogen or extraction from lyophilised plant tissues. Here, we evaluated the two using non-targeted metabolomics to show that lyophilisation can stabilise the maize (Zea mays) extractable metabolome, increasing throughput and efficiency of extraction as compared to the more traditional processing in liquid nitrogen. Then, we applied the lyophilisation approach to explore the effect of drought upon the maize metabolome in a non-targeted HRMS metabolomics approach. Metabolomics revealed differences in the mature maize metabolome having undergone three drought conditions imposed at two critical development stages (three-leaf stage and grain-fill stage); moreover, this difference was observed across two tissue types (kernel and inner cob/pith). It was shown that under ideal conditions, the biochemical make-up of the tissue types is different. However, under stress conditions, the stress response dominates the metabolic profile. Drought-related metabolites known from other plant systems have been identified and metabolomics has revealed potential novel drought-stress indicators in our maize system.</p></article>", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "abiotic stress", "1312", "2712", "1303", "15. Life on land", "maize", "metabolomics", "LC\u2013MS", "Microbiology", "QR1-502", "Article", "6. Clean water", "03 medical and health sciences", "climate change", "maize; metabolomics; LC\u2013MS; high-resolution mass spectrometry; abiotic stress; climate change", "high-resolution mass spectrometry"]}, "links": [{"href": "https://eprints.whiterose.ac.uk/175862/1/metabolites_11_00438.pdf"}, {"href": "http://www.mdpi.com/2218-1989/11/7/438/pdf"}, {"href": "https://doi.org/10.3390/metabo11070438"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Metabolites", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/metabo11070438", "name": "item", "description": "10.3390/metabo11070438", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/metabo11070438"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-07-03T00:00:00Z"}}, {"id": "10.3390/molecules25153495", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:20:44Z", "type": "Journal Article", "created": "2020-08-03", "title": "Phytochemical Characterization and In Vitro Antioxidant Properties of Four Brassica Wild Species from Italy", "description": "<p>In the present study, we evaluated for the first time the variability of antioxidant traits of four Brassica wild species: B. incana, B. macrocarpa, B. villosa, and B. rupestris. The content of the main water-soluble antioxidants (phenolics, ascorbic acid, and total biothiols) and the in vitro antioxidant potential (1,1-diphenyl-2-picrylhydrazil (DPPH) and superoxide anion scavenging capacity) were investigated. A total of 28 polyphenolic compounds were identified by LC/MS and quantitated by HPLC/DAD analysis. Kaempferol and quercetin derivatives were the most abundant phenolics compared to hydroxycinnamoyl gentiobiosides. In the ten populations, phenolics ranged from 163.9 to 533.9 mg/100 g dry weight (d.w.), ascorbic acid from 7.6 to 375.8 mg/100 g d.w., and total biothiols from 0.59 to 5.13 mg/100 g d.w. The different classes of phytochemicals were separated using solid-phase extraction at increasing methanol concentrations, and the antioxidant power of fractionated extracts was evaluated. The superoxide anion scavenging activity was significantly correlated to phenolics, particularly to flavonol derivatives, while DPPH was mainly related to ascorbic acid content. The present findings improve the knowledge of the phytochemical composition of Italian Brassica wild species by showing the great diversity of phytochemicals among populations and highlighting their importance as a valuable genetic resource for developing new cultivars with improved bioactive content.</p>", "keywords": ["Polyphenol", "0301 basic medicine", "Scavenging activity", "Phytochemicals", "610", "Organic chemistry", "Ascorbic Acid", "Brassica", "Brassica wild relatives", "630", "Article", "Antioxidants", "Mass Spectrometry", "LC-UV-PDA-ESI-MS", "03 medical and health sciences", "QD241-441", "Picrates", "Brassica wild relative", "scavenging activity", "polyphenols", "Chromatography", " High Pressure Liquid", "0303 health sciences", "Plant Extracts", "Biphenyl Compounds", "<i>Brassica</i> wild relatives", "Polyphenols", "Brassica wild relatives; DPPH; LC-UV-PDA-ESI-MS; polyphenols; scavenging activity", "3. Good health", "Italy", "Solubility", "Seeds", "DPPH"]}, "links": [{"href": "http://www.mdpi.com/1420-3049/25/15/3495/pdf"}, {"href": "https://iris.cnr.it/bitstream/20.500.14243/382555/1/prod_428450-doc_152843.pdf"}, {"href": "https://www.iris.unict.it/bitstream/20.500.11769/480328/2/Picchi%20et%20al%2c%202020%20Brassica%20phytochemicals%20molecules-25-03495.pdf"}, {"href": "https://www.mdpi.com/1420-3049/25/15/3495/pdf"}, {"href": "https://doi.org/10.3390/molecules25153495"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Molecules", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/molecules25153495", "name": "item", "description": "10.3390/molecules25153495", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/molecules25153495"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-07-31T00:00:00Z"}}, {"id": "10.5061/dryad.c85gk", "type": "Feature", "geometry": null, "properties": {"license": "unspecified", "updated": "2026-07-28T16:21:09Z", "type": "Dataset", "title": "Data from: Plant-soil interactions shape the identity and persistence of soil organic carbon in invaded ecosystems: implication for legacy effects", "description": "unspecified1. Introduced, invasive plants can alter local soil chemistry and  microbial communities, but the underlying mechanisms and extent of these  changes are largely unknown. Based on characteristics associated with  invasiveness in plants, it was hypothesized that introduced species that  produce large amounts of litter with distinctive secondary compounds can  a) alter the chemistry of both extractable and bulk carbon in the soil, b)  shift microbial communities towards microbes better able to metabolize the  compounds in the litter, and c) cause soil carbon chemistry and microbial  communities to shift to relatively uniform, novel states at multiple  sites. 2. Composition of phenolics in senescent tissues (leaves and roots)  of Polygonum cuspidatum was compared to the composition of extractable  phenolics and non-extractable bulk organic carbon in soils under and  adjacent to large, long-established stands of P. cuspidatum at four sites  in the eastern U.S. Rates of degradation of phenolics, activities of  enzymes associated with the breakdown of phenolics, and shifts in  microbial community composition were also measured at the sites. 3. Soils  under P. cuspidatum stands contained twice as much phenolics as adjacent  soils, but the composition of phenolics differed greatly between soils  under stands and senescent tissues of P. cuspidatum. Flavonoids and  proanthocyanidins constituted &gt;90% of the identified phenolics in  P. cuspidatum tissues, whereas monophenolic compounds accounted for  &gt; 90% of the phenolics in soils under stands. Soils under and  adjacent to stands also exhibited distinctive compositions of relatively  persistent bulk organic carbon; composition differed less between soils  under stands at different sites than between soils under and adjacent to  stands at the same site. 4. Soils under P. cuspidatum had 2.8 times  greater abundance of fungi than soils adjacent to stands, and fungal  markers showed clear separation of soils under and adjacent to P.  cuspidatum. However, the potential activity of enzymes that degrade  polyphenols was lower in soils under stands. Exogenously applied  chemically complex polyphenols persisted in both P. cuspidatum invaded and  adjacent non-invaded soils, whereas less complex compounds rapidly  disappeared from both soils. 5. Synthesis. Results suggest that  interactions between plant inputs, abiotic reactions, and biotic  transformations may create and maintain new states in invaded soils that  are chemically and biologically less diverse. In the case of polyphenol  rich, fast growing invasive species, these interactions may alter the  composition of bulk soil organic matter that has slower turnover rates,  resulting in legacy effects. Restoration could thus require, not just  removal of the species, but also post-removal interventions such as soil  amendments.", "keywords": ["Flavonoids", "Peroxidases", "Mass spectrometry", "tannins", "Fallopia japonica", "Japanese knotweed", "Reynoutria japonica", "Polyphenols", "home-field advantage", "Legacy effect", "15. Life on land", "soil enzymes"], "contacts": [{"organization": "Suseela, Vidya, Alpert, Peter, Nakatsu, Cindy H., Armstrong, Arthur, Tharayil, Nishanth,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.5061/dryad.c85gk"}, {"rel": "self", "type": "application/geo+json", "title": "10.5061/dryad.c85gk", "name": "item", "description": "10.5061/dryad.c85gk", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5061/dryad.c85gk"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-24T00:00:00Z"}}, {"id": "11381/2960672", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:24:04Z", "type": "Journal Article", "created": "2023-10-05", "title": "Ultra-high Performance Liquid Chromatography\u2013Ion Mobility\u2013High-Resolution Mass Spectrometry to Evaluate the Metabolomic Response of Durum Wheat to Sustainable Treatments", "description": "Sustainable agriculture aims at achieving a healthy food production while reducing the use of fertilizers and greenhouse gas emissions using biostimulants and soil amendments. Untargeted metabolomics by ultra-high performance liquid chromatography-ion mobility-high-resolution mass spectrometry, operating in a high-definition MSE mode, was applied to investigate the metabolome of durum wheat in response to sustainable treatments, i.e., the addition of biochar, commercial plant growth promoting microbes, and their combination. Partial least squares-discriminant analysis provided a good discrimination among treatments with sensitivity, specificity, and a non-error rate close to 1. A total of 88 and 45 discriminant compounds having biological, nutritional, and technological implications were tentatively identified in samples grown in 2020 and 2021. The addition of biochar-biostimulants produced the highest up-regulation of lipids and flavonoids, with the glycolipid desaturation being the most impacted pathway, whereas carbohydrates were mostly down-regulated. The findings achieved suggest the safe use of the combined biochar-biostimulant treatment for sustainable wheat cultivation.", "keywords": ["2. Zero hunger", "13. Climate action", "Settore AGRI-06/A - Genetica agraria", "615", "Metabolomics", "Settore CHEM-01/A - Chimica analitica", "Settore BIOS-10/A - Biologia cellulare e applicata", "630", "Chromatography", " High Pressure Liquid", "Triticum", "Mass Spectrometry", "12. Responsible consumption", "ultra-high performance liquid chromatography\u2212high-resolution mass spectrometry ion mobility untargeted metabolomics multivariate data analysis durum wheat biostimulants soil amendments"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.3c04532"}, {"href": "https://doi.org/11381/2960672"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Agricultural%20and%20Food%20Chemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11381/2960672", "name": "item", "description": "11381/2960672", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11381/2960672"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-05T00:00:00Z"}}, {"id": "11573/1696744", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:24:06Z", "type": "Journal Article", "created": "2023-09-08", "title": "Evaluation of Pure PFAS Decrease in Controlled Settings", "description": "Since 1940, poly- or perfluorinated alkyl substances (PFAS) have been largely used in many applications, including paints, fire foaming, household items, product packaging, and fabrics. Because of their extremely high persistency, they have been defined as 'forever chemicals'. Although the EU is taking action to reduce their use, their widespread occurrence in environmental matrices and their harmful effects on human health require the use of highly performing analytical methods for efficient monitoring. Furthermore, novel PFAS are constantly revealed by both EU and National environmental agencies. The objective of this work is to investigate the cause of the signal decrease during the analysis of a standard PFAS mixture in water-based matrices, by proposing an efficient technical procedure for laboratory specialists. The analyses were carried out on a mixture of 30 PFAS, including both regulated and unknown substances (which are expected to be introduced in the guidelines), characterized by different chemical features, using LC-vials of two different materials, namely, glass and polypropylene, and dissolved in two solvents, namely, water and water-methanol. The temperature of analysis and the concentration of PFAS were also considered through LC-MS analyses at different times, in the 0-15 h range. Depending on the chemical structure and length of the PFAS, sampling and treatment procedures may be adopted to tackle the decrease and the release from the containers, reducing the risk of underestimating PFAS also in real water matrices.", "keywords": ["pfas; mass spectrometry; adsorption; drinking water; containers", "QD71-142", "Analytical chemistry"]}, "links": [{"href": "https://iris.uniroma1.it/bitstream/11573/1696744/1/Mancini_Evaluation_2023.pdf"}, {"href": "https://pubs.acs.org/doi/pdf/10.1021/acsmeasuresciau.3c00027"}, {"href": "https://doi.org/11573/1696744"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ACS%20Measurement%20Science%20Au", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11573/1696744", "name": "item", "description": "11573/1696744", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11573/1696744"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-09-07T00:00:00Z"}}, {"id": "10256/20664", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:23:45Z", "type": "Journal Article", "created": "2021-08-07", "title": "Groundwater antibiotic pollution and its relationship with dissolved organic matter: Identification and environmental implications", "description": "The occurrence of veterinary antibiotics and hydro-chemical parameters in eleven natural springs in a livestock production area is evaluated, jointly with the characterization of their DOM fingerprint by Orbitrap HRMS. Tetracycline and sulfonamide antibiotics were ubiquitous in all sites, and they were detected at low ng L-1 concentrations, except for doxycycline, that was present at \u03bcg L-1 in one location. DOM analysis revealed that most molecular formulas were CHO compounds (49 %-68\u00a0%), with a remarkable percentage containing nitrogen and sulphur (16 %-23\u00a0% and 11 %-24\u00a0%, respectively). Major DOM components were phenolic and highly unsaturated compounds (~90\u00a0%), typical for soil-derived organic matter, while approximately 11\u00a0% were unsaturated aliphatic, suggesting that springs may be susceptible to anthropogenic contamination sources. Comparing the DOM fingerprint among sites, the spring showing the most different profile was the one with surface water interaction and characterized by having lower CHO and higher CHOS formulas and aliphatic compounds. Correlations between antibiotics and DOM showed that tetracyclines positively correlate with unsaturated oxygen-rich substances, while sulfonamides relate with aliphatic and unsaturated oxygen-poor compounds. This indicates that the fate of different antibiotics will be controlled by the type of DOM present in groundwater.", "keywords": ["High-resolution mass spectrometry", "550", "Contaminants emergents en l'aigua", "Antibi\u00f2tics", "02 engineering and technology", "01 natural sciences", "630", "Soil", "Antibiotics", "Groundwater -- Pollution", "Co-transport", "Dissolved organic matter", "Groundwater", "0105 earth and related environmental sciences", "2. Zero hunger", "[SDU.OCEAN]Sciences of the Universe [physics]/Ocean", "Emerging contaminants in water", "Atmosphere", "[SDU.OCEAN] Sciences of the Universe [physics]/Ocean", " Atmosphere", "[SDU.ENVI] Sciences of the Universe [physics]/Continental interfaces", " environment", "6. Clean water", "Anti-Bacterial Agents", "Water quality", "13. Climate action", "Aig\u00fces subterr\u00e0nies -- Contaminaci\u00f3", "[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces", "0210 nano-technology", "environment", "Water Pollutants", " Chemical", "Environmental Monitoring"]}, "links": [{"href": "https://doi.org/10256/20664"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10256/20664", "name": "item", "description": "10256/20664", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10256/20664"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-11-01T00:00:00Z"}}, {"id": "11568/935316", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:24:06Z", "type": "Journal Article", "created": "2018-05-09", "title": "Chronological Classification of Ancient Mortars Employing Spectroscopy and Spectrometry Techniques: Sagunto (Valencia, Spain) Case", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Forty-two mortar samples, from two archaeological excavations located in Sagunto (Valencian Community, Spain), were analysed by both portable energy dispersive X-ray fluorescence spectroscopy (pED-XRF) and inductively coupled plasma mass spectrometry (ICP-MS) to determine major and minor elements and traces including rare earth elements (REEs). Collected data were crossed with those previously obtained from Sagunto Castle mortars, and principal component analysis (PCA) was applied to discriminate the construction phases of the unearthed buildings. REE permitted to ascribe most of the masonries to the Roman Imperial period. Moreover, a statistical model was built by employing partial least squares discriminant analysis (PLS-DA) in order to classify the mortars from Roman Imperial period and from Islamic period due to the problematic overlapping between these two phases. Results confirmed the effectiveness of the developed indirect chronology method, based on REE data, to discriminate among historic mortars from different construction periods on a wide scale including different Sagunto archaeological sites.</p></article>", "keywords": ["2. Zero hunger", "0601 history and archaeology", "QC350-467", "06 humanities and the arts", "Optics. Light", "energy dispersive X-ray fluorescence spectroscopy (pED-XRF); inductively coupled plasma mass spectrometry (ICP-MS)", "Analytical Chemistry; Atomic and Molecular Physics", " and Optics; Spectroscopy"]}, "links": [{"href": "https://eprints.whiterose.ac.uk/130462/1/9736547.pdf"}, {"href": "https://iris.unica.it/bitstream/11584/248342/2/Ramacciotti%20et%20al%202018.pdf"}, {"href": "https://arpi.unipi.it/bitstream/11568/935316/1/P101%20Chronological%20Classification%20of%20Ancient%20Mortars.pdf"}, {"href": "https://doi.org/11568/935316"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Spectroscopy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11568/935316", "name": "item", "description": "11568/935316", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11568/935316"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-01-01T00:00:00Z"}}, {"id": "11579/201465", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:24:07Z", "type": "Journal Article", "created": "2024-12-05", "title": "Sensitive and accurate determination of 32 PFAS in human serum using online SPE-UHPLC-HRMS", "description": "Per- and polyfluoroalkyl substances' (PFAS) extreme persistence has been linked to many adverse effects on human health including increased risk of certain cancers. This study presents the development and validation of a new, highly sensitive method for the quantification of 32 PFAS in human serum using online solid-phase extraction (SPE) coupled with ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Legacy and emerging PFAS were targeted. Main steps of sample pretreatment include protein precipitation (PP), pellet rinsing, centrifugation, preconcentration through solvent evaporation, and online SPE using a weak anion-exchange polymeric sorbent. The PP and pellet-rinsing procedures were optimized through a comprehensive exploration of solvent combinations. Following this, a pretreatment that offers the best compromise for the targeted PFAS was identified using principal component analysis. The method demonstrated excellent linearity (R\u00b2 = 0.977-0.997) with limits of quantification ranging from 8.9 to 27\u00a0ng/L, 5 to 15 times lower than previous methods. Precision (intraday 2.6-14.0\u00a0% and interday 1.3-11.0\u00a0% relative standard deviation) and accuracy (recoveries 72.7-106\u00a0%) were robust. The method was validated in accordance with ISO/IEC 17025 and successfully applied to five human serum samples, confirming its suitability for high-throughput profiling of PFAS in biomonitoring studies. This method is the first to use online SPE for the simultaneous determination of a broad range of PFAS, including ether congeners such as perfluoro(2-ethoxyethane) sulfonic acid and Nafion byproduct 2. Furthermore, control charts were employed to assess instrument performance during routine analysis and implement necessary actions.", "keywords": ["Human biomonitoring", "Fluorocarbons", "PFAS", "biomonitoring", "Method development", "Solid Phase Extraction", "628", "Humans", "Serum pretreatment", "High resolution mass spectrometry", "Chromatography", " High Pressure Liquid", "Mass Spectrometry", "543"]}, "links": [{"href": "https://iris.univr.it/bitstream/11562/1159353/1/2025%20Sensitive%20and%20accurate%20determination%20of%2032%20PFAS%20in%20human%20serum%20using%20SPE-UHPLC-HRMS.pdf"}, {"href": "https://doi.org/11579/201465"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Hazardous%20Materials", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11579/201465", "name": "item", "description": "11579/201465", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11579/201465"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-03-01T00:00:00Z"}}, {"id": "11585/582157", "type": "Feature", "geometry": null, "properties": {"license": "Restricted", "updated": "2026-07-28T16:24:08Z", "type": "Journal Article", "created": "2017-01-04", "title": "The cost of surviving nitrogen excess: energy and protein demand in the lichen Cladonia portentosa as revealed by proteomic analysis", "description": "Different nitrogen forms affect different metabolic pathways in lichens. In particular, the most relevant changes in protein expression were observed in the fungal partner, with NO 3- mostly affecting the energetic metabolism and NH 4+ affecting transport and regulation of proteins and the energetic metabolism much more than NO 3- did. Excess deposition of reactive nitrogen is a well-known agent of stress for lichens, but which symbiont is most affected and how, remains a mystery. Using proteomics can expand our understanding of stress effects on lichens. We investigated the effects of different doses and forms of reactive nitrogen, with and without supplementary phosphorus and potassium, on the proteome of the lichen Cladonia portentosa growing in a 'real-world' simulation of nitrogen deposition. Protein expression changed with the nitrogen treatments but mostly in the fungal partner, with NO3- mainly affecting the energetic metabolism and NH4+ also affecting the protein synthesis machinery. The photobiont mainly responded overexpressing proteins involved in energy production. This suggests that in response to nitrogen stress, the photobiont mainly supports the defensive mechanisms initiated by the mycobiont with an increased energy production. Such surplus energy is then used by the cell to maintain functionality in the presence of NO3-, while a futile cycle of protein production can be hypothesized to be induced by NH4+ excess. External supply of potassium and phosphorus influenced differently the responses of particular enzymes, likely reflecting the many processes in which potassium exerts a regulatory function.", "keywords": ["Chlorophyll", "Proteomics", "0301 basic medicine", "570", "mycobiont", "Lichens", "Nitrogen", "Cell Respiration", "Nitrate", "Mass Spectrometry", "Molecular mechanism", "03 medical and health sciences", "nitrate", "Ammonia", "Electrophoresis", " Gel", " Two-Dimensional", "Photosynthesis", "Ammonium", " Molecular mechanism", " Mycobiont", " Nitrate", " Photobiont", " Stress response", "Ammonium; Molecular mechanism; Mycobiont; Nitrate; Photobiont; Stress response; Genetics; Plant Science", "0303 health sciences", "Nitrates", "Stress response", "Chlorophyll A", "stress response", "Mycobiont", "ammonium", "Photobiont", "photobiont", "molecular mechanism", "Energy Metabolism", "Ammonium"]}, "links": [{"href": "http://link.springer.com/content/pdf/10.1007/s00425-017-2647-2.pdf"}, {"href": "https://doi.org/11585/582157"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Planta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11585/582157", "name": "item", "description": "11585/582157", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11585/582157"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-04T00:00:00Z"}}, {"id": "2158/1353729", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:24:38Z", "type": "Journal Article", "created": "2019-03-25", "title": "Chemical and mineralogical analyses on stones from Sagunto Castle (Spain)", "description": "For the first time, an archaeometric study was carried out on the carbonate rock ashlars of the Sagunto Castle. The studied site is one of the most important and best preserved Spanish archaeological and architectural monuments, characterized by different construction phases from the Roman period to Modern Ages. Forty samples collected from thirteen different structures of Sagunto Castle and two quarries, located in the Sagunto's hill were used for comparative purposes. The samples were analyzed by X-ray diffraction, X-ray fluorescence and inductively coupled plasma mass spectrometry to determine their mineralogical and elemental composition. The obtained data show similar chemical and mineralogical features between the rocks outcropping in the city quarries and some of those employed to build the structures, suggesting that rocks could have been used to build the structures from different periods along the centuries.", "keywords": ["Building stone; Chemistry; Fortress; Middle ages; Mineralogy; Remains; Roman period", "Building stone; Fortress; Remains; Chemistry; Mineralogy; Roman period; Middle ages", "archaeometrics; carbonate rock ashlars; X-ray fluorescence and inductively coupled plasma mass spectrometry", "01 natural sciences", "0104 chemical sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://iris.unica.it/bitstream/11584/298931.2/3/Ramaciotti%20et%20al%202018_1-s2.0-S2352409X18307454-main.pdf"}, {"href": "https://doi.org/2158/1353729"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Archaeological%20Science%3A%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2158/1353729", "name": "item", "description": "2158/1353729", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2158/1353729"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-01T00:00:00Z"}}, {"id": "21645685", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:24:39Z", "type": "Journal Article", "created": "2011-04-21", "title": "Development of a solvent-free method for the simultaneous identification/quantification of drugs of abuse and their metabolites in environmental water by LC\u2013MS/MS", "description": "This work details a rapid analytical method using direct sample injection for the simultaneous identification/quantification of 22 drugs of abuse, including some of their major metabolites, in environmental samples. This has been developed using a hybrid triple quadrupole-linear ion trap-mass spectrometer (QqLIT). With the increasing sensitivity of today's tandem mass spectrometers, direct injection analysis of water samples has become an attractive alternative to traditional analytical protocols, which often include a preliminary pre-concentration step. What's more, this kind of analysis is in accordance with many of the main objectives of so-called green analytical chemistry, or environmentally friendly practice. The analytical performance of the LC-MS/MS method was evaluated in three different water matrices (surface water, influent and effluent wastewater). Data acquisition was carried out in selected reaction monitoring (SRM) mode under time-scheduled conditions, monitoring two SRM transitions for simultaneous identification/quantification of all target compounds in the samples. Additionally, an experiment was performed using the information-dependent acquisition (IDA) scan to carry out the identification of those analytes for which the second transition was present at a low intensity. Finally, the two methodologies developed were applied to real samples for evaluation.", "keywords": ["Illicit Drugs", "Tandem Mass Spectrometry", "Solid Phase Extraction", "TD Environmental technology. Sanitary engineering", "Environmental Pollutants", "TP Chemical technology", "01 natural sciences", "Water Pollutants", " Chemical", "6. Clean water", "Chromatography", " Liquid", "Environmental Monitoring", "0104 chemical sciences", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/21645685"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Talanta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "21645685", "name": "item", "description": "21645685", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/21645685"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-07-01T00:00:00Z"}}, {"id": "3161788824", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-28T16:25:10Z", "type": "Journal Article", "created": "2021-05-19", "title": "An Automated Methodology for Non-targeted Compositional Analysis of Small Molecules in High Complexity Environmental Matrices Using Coupled Ultra Performance Liquid Chromatography Orbitrap Mass Spectrometry", "description": "<strong>Abstract</strong> The life-critical matrices of air and water are among the most complex chemical mixtures that are ever encountered. Ultra-high resolution mass spectrometers, such as the Orbitrap, provide unprecedented analytical capabilities to probe the molecular composition of such matrices, but the extraction of non-targeted chemical information is impractical to perform <em>via</em> manual data processing. Automated non-targeted tools rapidly extract the chemical information of all detected compounds within a sample dataset. However, these methods have not been exploited in the environmental sciences. Here, we provide an automated and (for the first time) rigorously tested methodology for the non-targeted compositional analysis of environmental matrices using coupled liquid chromatography-mass spectrometric data. First, the robustness and reproducibility was tested using authentic standards, evaluating performance as a function of concentration, ionization potential and sample complexity. The method was then used for the compositional analysis of particulate matter and surface waters collected from world-wide locations. The method detected &gt;9,600 compounds in the individual environmental samples, arising from critical pollutant sources, including carcinogenic industrial chemicals, pesticides, pharmaceuticals,<em> </em>among others. This methodology offers considerable advances in the environmental sciences, providing a more complete assessment of sample compositions, whilst significantly increasing throughput.", "keywords": ["13. Climate action", "1600", "2304", "Reproducibility of Results", "Pesticides", "01 natural sciences", "Chromatography", " High Pressure Liquid", "Mass Spectrometry", "Water Pollutants", " Chemical", "Chromatography", " Liquid", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://eprints.bournemouth.ac.uk/36790/1/An%20Automated%20Methodology%20for%20Non-targeted%20Compositional%20Analysis%20of%20Small%20Molecules%20in%20High%20Complexity%20Environmental%20Matrice.pdf"}, {"href": "https://eprints.whiterose.ac.uk/174399/1/acs.est.0c08208.pdf"}, {"href": "https://doi.org/3161788824"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "3161788824", "name": "item", "description": "3161788824", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/3161788824"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-05-18T00:00:00Z"}}, {"id": "3182395921", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:25:12Z", "type": "Journal Article", "created": "2021-07-05", "title": "Metabolomic Approaches to Studying the Response to Drought Stress in Corn (Zea mays) Cobs", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>Metabolomics is a technique that allows for the evaluation of the entire extractable chemical profile of a plant, for example, using high-resolution mass spectrometry (HRMS) and can be used to evaluate plant stress responses, such as those due to drought. Metabolomic analysis is dependent upon the efficiency of the extraction protocol. Currently, there are two common extraction procedures widely used in metabolomic experiments, those that extract from plant tissue processed in liquid nitrogen or extraction from lyophilised plant tissues. Here, we evaluated the two using non-targeted metabolomics to show that lyophilisation can stabilise the maize (Zea mays) extractable metabolome, increasing throughput and efficiency of extraction as compared to the more traditional processing in liquid nitrogen. Then, we applied the lyophilisation approach to explore the effect of drought upon the maize metabolome in a non-targeted HRMS metabolomics approach. Metabolomics revealed differences in the mature maize metabolome having undergone three drought conditions imposed at two critical development stages (three-leaf stage and grain-fill stage); moreover, this difference was observed across two tissue types (kernel and inner cob/pith). It was shown that under ideal conditions, the biochemical make-up of the tissue types is different. However, under stress conditions, the stress response dominates the metabolic profile. Drought-related metabolites known from other plant systems have been identified and metabolomics has revealed potential novel drought-stress indicators in our maize system.</p></article>", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "abiotic stress", "1312", "2712", "1303", "15. Life on land", "maize", "metabolomics", "LC\u2013MS", "Microbiology", "QR1-502", "Article", "6. Clean water", "03 medical and health sciences", "climate change", "maize; metabolomics; LC\u2013MS; high-resolution mass spectrometry; abiotic stress; climate change", "high-resolution mass spectrometry"]}, "links": [{"href": "https://eprints.whiterose.ac.uk/175862/1/metabolites_11_00438.pdf"}, {"href": "http://www.mdpi.com/2218-1989/11/7/438/pdf"}, {"href": "https://doi.org/3182395921"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Metabolites", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "3182395921", "name": "item", "description": "3182395921", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/3182395921"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-07-03T00:00:00Z"}}, {"id": "37709429", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-28T16:25:26Z", "type": "Journal Article", "created": "2023-08-15", "title": "Surveying the mugineic acid family: Ion mobility \u2013 quadrupole time-of-flight mass spectrometry (IM-QTOFMS) characterization and tandem mass spectrometry (LC-ESI-MS/MS) quantification of all eight naturally occurring phytosiderophores", "description": "Phytosiderophores (PS) are root exudates released by grass species (Poaceae) that play a pivotal role in iron (Fe) plant nutrition. A direct determination of PS in biological samples is of paramount importance in understanding micronutrient acquisition mediated by PS. To date, eight plant-born PS have been identified; however, no analytical procedure is currently available to quantify all eight PS simultaneously with high analytical confidence. With access to the full set of PS standards for the first time, we report comprehensive methods to both fully characterize (IM-QTOFMS) and quantify (LC-ESI-MS/MS) all eight naturally occurring PS belonging to the mugineic acid family. The quantitative method was fully validated, yielding linear results for all eight analytes, and no unwanted interferences with soil and plant matrices were observed. LOD and LOQ values determined for each PS were below 11 and 35\u00a0nmol\u00a0L-1, respectively. The method's precision under reproducibility conditions (intra- and inter-day) of measurement was less than 2.5% RSD for all analytes. Additionally, all PS were annotated with high-resolution mass spectrometric fragment spectra and further characterized via drift tube ion mobility-mass spectrometry. The collision cross-sections obtained for primary ion species yielded a valuable database for future research focused on in-depth PS studies. The new quantitative method was applied to analyse root exudates from Fe-controlled and deficient barley, oat, rye, and sorghum plants. All eight PS, including mugineic acid (MA), 3'-hydroxymugineic acid (HMA), 3'-epi-hydroxymugineic acid (epi-HMA), hydroxyavenic acid (HAVA), deoxymugineic acid (DMA), 3'-hydroxydeoxymugineic acid (HDMA), 3'-epi-hydroxydeoxymugineic acid (epi-HDMA) and avenic acid (AVA) were for the first time successfully identified and quantified in root exudates of various graminaceous plants using a single analytical procedure. These newly developed methods can be applied to studies aimed at improving crop yield and micronutrient grain content for food consumption via plant-based biofortification.", "keywords": ["Tandem Mass Spectrometry", "Reproducibility of Results", "Micronutrients", "Poaceae", "Edible Grain"]}, "links": [{"href": "https://doi.org/37709429"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Analytica%20Chimica%20Acta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "37709429", "name": "item", "description": "37709429", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/37709429"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-10-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Mass+Spectrometry&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Mass+Spectrometry&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Mass+Spectrometry&", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Mass+Spectrometry&offset=50", "hreflang": "en-US"}], "numberMatched": 59, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-07-28T20:11:45.117558Z"}