{"type": "FeatureCollection", "features": [{"id": "10.1094/pdis-06-21-1276-pdn", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:17:18Z", "type": "Journal Article", "created": "2022-01-10", "title": "First Report of Multinucleate Rhizoctonia solani AG4 HG-I Causing Crown and Root Rot on Strawberry in Italy", "description": "Strawberry (Fragaria\u00a0\u00d7\u00a0ananassa\u00a0Duch.) is a crop of great economic importance in Italy, where it is grown in soil and under soilless conditions. In March 2019, about 30 to 35% of plants (cv. Portola) grown in a peat substrate under soilless conditions in a farm located in Cuneo Province died. The examination of 10 plants showed crown and root rot over 100% of the root/crown. Affected plants showed brown necrotic tissues in basal leaves and petiole necrosis. Crown and root tissues were cleaned thoroughly from soil residues under tap water. Portions (about 3 to 5 mm) from crowns and roots were cut and surface disinfected with a water solution of NaClO at 0.5% for 2 min and rinsed in sterile water. The tissue fragments were plated on potato dextrose agar (PDA) amended with 100 mg/liter of streptomycin sulfate and incubated at 25\u00b0C. After 3 days, fungal colonies with septate hyphae and right-angled branching similar to\u00a0Rhizoctonia solani\u00a0were observed with high frequency (90%) (Sneh et\u00a0al. 1991). To confirm the species identity, hyphal tips were transferred from the obtained colonies to PDA and grown for 10 days at 22 \u00b1 1\u00b0C. Mycelium was light brown, compact, with radial growth. The hyphal width varied from 8.5 to 10 \u03bcm. Sclerotia were not present. DNA was then extracted from a single representative isolate (RH230), and rDNA ITS sequencing was conducted as described by\u00a0Aiello et\u00a0al. (2017). The rDNA ITS sequence of RH230 (GenBank accession no. MZ373271) was 100% identical (603/603 bp) to part of another sequence previously identified as\u00a0R. solani\u00a0AG4 HG-I (MK583647,\u00a0Claerbout et\u00a0al. 2019). Twenty-day-old healthy plants of cultivar Portola were planted in a steam-disinfested peat soil (12-liter pots) infested with 1 g/liter of wheat kernels colonized for 10 days with the isolate RH230 to evaluate the pathogenicity. Control plants were planted in a steam-disinfested peat substrate amended with noninoculated sterilized wheat kernels. Six plants per treatments were used and kept in a greenhouse at 25 \u00b1 3\u00b0C. Crown and root rot similar to that observed in the farm developed 40 to 55 days after inoculation and resulted in 50 to 66% dead plants during two repeated trials. Fungal colonies morphologically similar to\u00a0R. solani\u00a0were consistently reisolated from affected crowns, and the resequencing of the rDNA ITS region fulfilled Koch\u2019s postulates. Control plants remained healthy.\u00a0Rhizoctonia\u00a0isolates of AG-A and AG-G anastomosis groups were found as pathogens of strawberry in Italy (Manici and Bonora 2007), while the AG4 HG-I was reported in Israel (Sharon et\u00a0al. 2007).\u00a0R. solani\u00a0AG4 HG-I was found on other hosts (Aiello et\u00a0al. 2017); however, to our knowledge, this is the first report on strawberry in Italy. The disease could become a significant problem for soilless culture strawberry in Italy, causing severe yield losses.", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Crown rot; Rhizoctonia; Root rot; Strawberry", "15. Life on land", "6. Clean water"]}, "links": [{"href": "https://doi.org/10.1094/pdis-06-21-1276-pdn"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Plant%20Disease", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1094/pdis-06-21-1276-pdn", "name": "item", "description": "10.1094/pdis-06-21-1276-pdn", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1094/pdis-06-21-1276-pdn"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-01T00:00:00Z"}}, {"id": "10.1111/j.1365-2486.2010.02318.x", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:17:37Z", "type": "Journal Article", "created": "2010-08-29", "title": "Long-Term Experimental Manipulation Of Climate Alters The Ectomycorrhizal Community Of Betula Nana In Arctic Tundra", "description": "Climate warming is leading to shrub expansion in Arctic tundra. Shrubs form ectomycorrhizal (ECM) associations with soil fungi that are central to ecosystem carbon balance as determinants of plant community structure and as decomposers of soil organic matter. To assess potential climate change impacts on ECM communities, we analysed fungal internal transcribed spacer sequences from ECM root tips of the dominant tundra shrub Betula nana growing in treatments plots that had received long-term warming by greenhouses and/or fertilization as part of the Arctic Long-Term Ecological Research experiment at Toolik Lake Alaska, USA. We demonstrate opposing effects of long-term warming and fertilization treatments on ECM fungal diversity; with warming increasing and fertilization reducing the diversity of ECM communities. We show that warming leads to a significant increase in high biomass fungi with proteolytic capacity, especially Cortinarius spp., and a reduction of fungi with high affinities for labile N, especially Russula spp. In contrast, fertilization treatments led to relatively small changes in the composition of the ECM community, but increased the abundance of saprotrophs. Our data suggest that warming profoundly alters nutrient cycling in tundra, and may facilitate the expansion of B. nana through the formation of mycorrhizal networks of larger size.", "keywords": ["0106 biological sciences", "0301 basic medicine", "03 medical and health sciences", "13. Climate action", "15. Life on land", "01 natural sciences"]}, "links": [{"href": "https://doi.org/10.1111/j.1365-2486.2010.02318.x"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/j.1365-2486.2010.02318.x", "name": "item", "description": "10.1111/j.1365-2486.2010.02318.x", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/j.1365-2486.2010.02318.x"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2010-10-01T00:00:00Z"}}, {"id": "10.11591/.v3i4.4696", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:17:55Z", "type": "Journal Article", "created": "2019-11-04", "title": "Factors associated with Institutional delivery in Boricha district of Sidama zone, southern Ethiopia", "description": "

<p style='color: #000000; font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 10px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;'>Every year, 40 million women give birth at home without the help of a<br />skilled birth attendant. In 2011, 287,000 women died during pregnancy or childbirth. Almost all these deaths occur in developing countries where mothers and children lack access to basic health care. Reports showed the low utilization of health facility for delivery service in Ethiopia. This study aimed to determine the utilization and factors influencing institutional delivery. Community based cross sectional study was conducted from January to February 2013/14 in Boricha District of Southern Ethiopia among mothers who gave birth in the last 1 year. Multistage sampling techniques were used to collect data from 546 mothers. Taking in to account place of birth for the last child, only 4.9% women gave birth in a health facility. Women\u2019s education level (AOR=4.4 (95% CI=1.36-14.33)), timing of firstANC visit (AOR= .03 (95% CI=0.004 - 0.205)), women\u2019s advice to deliver in a health facility during ANC (AOR = 31.15 (95% CI=2.02-479.52)), women\u2019s knowledge of birth related complications (AOR= 12.4 (95% CI=2.67-57.16)) and decision making power (AOR=0.2 (95% CI=0.060.82)) showed significant association with institutionional delivery. Institutional delivery in the study area was found to be very low. Raising\u00a0awareness on institutional delivery to maximize delivery service utilization and strengthening provision of education and counseling to deliver in health facility during antenatal care visits at individual and community level should be given due emphasis.</p>

", "keywords": ["2. Zero hunger", "03 medical and health sciences", "0302 clinical medicine", "1. No poverty", "3. Good health"]}, "links": [{"href": "https://doi.org/10.11591/.v3i4.4696"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Public%20Health%20Science%20%28IJPHS%29", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.11591/.v3i4.4696", "name": "item", "description": "10.11591/.v3i4.4696", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.11591/.v3i4.4696"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-12-01T00:00:00Z"}}, {"id": "10.3389/fbioe.2019.00378", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:18:55Z", "type": "Journal Article", "created": "2019-12-04", "title": "Thickness of Polyelectrolyte Layers of Separately Confined Bacteria Alters Key Physiological Parameters on a Single Cell Level", "description": "Confinement of bacterial cells in a matrix or in capsules is an integral part of many biotechnological applications. Here, the well-known layer-by-layer method of deposition of a polyelectrolyte film a few nanometers in thickness to confine separated bacterial cells in permeable and physically durable shells has been examined. Due to the physical properties of such a confinement, we found that this method enables investigation of effects of physical barriers against mass gain and cell division. Using the method of time-lapse confocal microscopy, we observed a prolonged lag phase, dependent on the number of polyelectrolyte layers. In the confinement, both the GFP fluorescent signal from the leaking T7 promoter and the cell size were increased by factors of more than five and two, respectively. This creates a paradigm shift that enables use of mechanical entrapment for control of bacterial cell physiology and opens possibilities of controlling the division rate as well as gene expression. These effects can be attributed to the perturbation of the sensing of the cell size, which results in disproportional synthesis of a cell envelope impinging the intracellular material and compels cells to grow rapidly. In addition, the charged surface of cells enables prolonged intercellular physical interaction and results in spherically shaped microcolonies.", "keywords": ["polyelectrolytes", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "layer-by-layer encapsulation", "time-lapse confocal microscopy", " polyelectrolytes", " layer-by-layer encapsulation", " electrostatic interactions", " cell surface modification", "cell surface modification", "Bioengineering and Biotechnology", "electrostatic interactions", "time-lapse confocal microscopy", "TP248.13-248.65", "Biotechnology"]}, "links": [{"href": "https://doi.org/10.3389/fbioe.2019.00378"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Bioengineering%20and%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fbioe.2019.00378", "name": "item", "description": "10.3389/fbioe.2019.00378", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fbioe.2019.00378"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-12-04T00:00:00Z"}}, {"id": "10.3389/fmicb.2016.01446", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:18:57Z", "type": "Journal Article", "created": "2016-09-14", "description": "Soil management is fundamental to all agricultural systems and fertilization practices have contributed substantially to the impressive increases in food production. Despite the pivotal role of soil microorganisms in agro-ecosystems, we still have a limited understanding of the complex response of the soil microbiota to organic and mineral fertilization in the very long-term. Here, we report the effects of different fertilization regimes (mineral, organic and combined mineral and organic fertilization), carried out for more than a century, on the structure and activity of the soil microbiome. Organic matter content, nutrient concentrations, and microbial biomass carbon were significantly increased by mineral, and even more strongly by organic fertilization. Pyrosequencing revealed significant differences between the structures of bacterial and fungal soil communities associated to each fertilization regime. Organic fertilization increased bacterial diversity, and stimulated microbial groups (Firmicutes, Proteobacteria, and Zygomycota) that are known to prefer nutrient-rich environments, and that are involved in the degradation of complex organic compounds. In contrast, soils not receiving manure harbored distinct microbial communities enriched in oligotrophic organisms adapted to nutrient-limited environments, as Acidobacteria. The fertilization regime also affected the relative abundances of plant beneficial and detrimental microbial taxa, which may influence productivity and stability of the agroecosystem. As expected, the activity of microbial exoenzymes involved in carbon, nitrogen, and phosphorous mineralization were enhanced by both types of fertilization. However, in contrast to comparable studies, the highest chitinase and phosphatase activities were observed in the solely mineral fertilized soil. Interestingly, these two enzymes showed also a particular high biomass-specific activities and a strong negative relation with soil pH. As many soil parameters are known to change slowly, the particularity of unchanged fertilization treatments since 1902 allows a profound assessment of linkages between management and abiotic as well as biotic soil parameters. Our study revealed that pH and TOC were the majors, while nitrogen and phosphorous pools were minors, drivers for structure and activity of the soil microbial community. Due to the long-term treatments studied, our findings likely represent permanent and stable, rather than transient, responses of soil microbial communities to fertilization.", "keywords": ["Soil nutrients", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "long-term fertilization", "microbial biomass", "15. Life on land", "microbial activity", "Microbiology", "QR1-502", "03 medical and health sciences", "13. Climate action", "soil microbial communities", "soil nutrients", "454 pyrosequencing"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2016.01446"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2016.01446", "name": "item", "description": "10.3389/fmicb.2016.01446", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2016.01446"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-14T00:00:00Z"}}, {"id": "10.3389/fmicb.2019.02597", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:18:57Z", "type": "Journal Article", "created": "2019-11-08", "title": "New Insights Into Cinnamoyl Esterase Activity of Oenococcus oeni.", "description": "Some strains of Oenococcus oeni possess cinnamoyl esterase activity that can be relevant in the malolactic stage of wine production liberating hydroxycinnamic acids that are precursors of volatile phenols responsible for sensory faults. The objective of this study was to better understand the basis of the differential activity between strains. After initial screening, five commercial strains of O. oeni were selected, three were found to exhibit cinnamoyl esterase activity (CE+) and two not (CE-). Although the use of functional annotation of genes revealed genotypic variations between the strains, no specific genes common only to the three CE+ strains could explain the different activities. Pasteurized wine was used as a natural source of tartrate esters in growth and metabolism experiments conducted in MRS medium, whilst commercial trans-caftaric acid was used as substrate for enzyme assays. Detoxification did not seem to be the main biological mechanism involved in the activity since unlike its phenolic cleavage products and their immediate metabolites (trans-caffeic acid and 4-ethylcatechol), trans-caftaric acid was not toxic toward O. oeni. In the case of the two CE+ strains OenosTM and CiNeTM, wine-exposed samples showed a more rapid degradation of trans-caftaric acid than the unexposed ones. The CE activity was present in all cell-free extracts of both wine-exposed and unexposed strains, except in the cell-free extracts of the CE- strain CH11TM. This activity may be constitutive rather than induced by exposure to tartrate esters. Trans-caftaric acid was totally cleaved to trans-caffeic acid by cell-free extracts of the three CE+ strains, whilst cell-free extracts of the CE- strain CH16TM showed significantly lower activity, although higher for the strains in experiments with no prior wine exposure. The EstB28 esterase gene, found in the genomes of the 5 strains, did not reveal any difference on the upstream regulation and transport functionality between the strains. This study highlights the complexity of the basis of this activity in wine related O. oeni population. Variable cinnamoyl esterases or/and membrane transport activities in the O. oeni strains analyzed and a possible implication of wine molecules could explain this phenomenon.", "keywords": ["0301 basic medicine", "0303 health sciences", "tartrate esters", "cinnamoyl esterase", "Tartrate esters", "Hydroxycinnamic acids", "Wine", "hydroxycinnamic acids", "[SDV.IDA] Life Sciences [q-bio]/Food engineering", "Microbiology", "QR1-502", "03 medical and health sciences", "Cinnamoyl esterase", "wine", "Oenococcus oeni"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2019.02597"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2019.02597", "name": "item", "description": "10.3389/fmicb.2019.02597", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2019.02597"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-11-08T00:00:00Z"}}, {"id": "10.3389/fmicb.2021.758395", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:18:57Z", "type": "Journal Article", "created": "2022-01-12", "title": "Fresh rumen liquid inoculant enhances the rumen microbial community establishment in pre-weaned dairy calves", "description": "

The development of the functional rumen in calves involves a complex interplay between the host and host-related microbiome. Attempts to modulate rumen microbial community establishment may therefore have an impact on weaning success, calf health, and animal performance later in life. In this experiment, we aimed to elucidate how rumen liquid inoculum from an adult cow, provided to calves during the pre-weaning period, influences the establishment of rumen bacterial, archaeal, fungal, and ciliate protozoan communities in monozygotic twin calves (n = 6 pairs). The calves were divided into treatment (T-group) and control (C-group) groups, where the T-group received fresh rumen liquid as an oral inoculum during a 2\uffe2\uff80\uff938-week period. The C-group was not inoculated. The rumen microbial community composition was determined using bacterial and archaeal 16S ribosomal RNA (rRNA) gene, protozoal 18S rRNA gene, and fungal ITS1 region amplicon sequencing. Animal weight gain and feed intake were monitored throughout the experiment. The T-group tended to have a higher concentrate intake (Treatment: p &lt; 0.08) and had a significantly higher weekly weight gain (Treatment: p &lt; 0.05), but no significant difference in volatile fatty acid concentrations between the groups was observed. In the T-group, the inoculum stimulated the earlier establishment of mature rumen-related bacterial taxa, affecting significant differences between the groups until 6 weeks of age. The inoculum also increased the archaeal operational taxonomic unit (OTU) diversity (Treatment: p &lt; 0.05) but did not affect the archaeal quantity. Archaeal communities differed significantly between groups until week 4 (p = 0.02). Due to the inoculum, ciliate protozoa were detected in the T-group in week 2, while the C-group remained defaunated until 6 weeks of age. In week 8, Eremoplastron dilobum was the dominant ciliate protozoa in the C-group and Isotricha sp. in the T-group, respectively. The Shannon diversity of rumen anaerobic fungi reduced with age (Week: p &lt; 0.01), and community establishment was influenced by a change of diet and potential interaction with other rumen microorganisms. Our results indicate that an adult cow rumen liquid inoculum enhanced the maturation of bacterial and archaeal communities in pre-weaning calves\uffe2\uff80\uff99 rumen, whereas its effect on eukaryotic communities was less clear and requires further investigation.

", "keywords": ["0301 basic medicine", "570", "Anaerobic fungi", "microbiome establishment", "archaea", "Rumen function", "Microbiome manipulation", "Microbiology", "630", "Microbiome establishment", "ciliate protozoa", "03 medical and health sciences", "Dairy calf", "anaerobic fungi", "bacteria", "[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology", "2. Zero hunger", "0303 health sciences", "ta412", "Bacteria", "dairy calf", "Animal science", " dairy science", "Archaea", "Ciliate protozoa", "QR1-502", "rumen function", "[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology", "microbiome manipulation"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2021.758395"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2021.758395", "name": "item", "description": "10.3389/fmicb.2021.758395", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2021.758395"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-01-12T00:00:00Z"}}, {"id": "10.3389/fmicb.2022.983823", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:18:58Z", "type": "Journal Article", "created": "2022-11-08", "title": "Long-term effects of early-life rumen microbiota modulation on dairy cow production performance and methane emissions", "description": "

Rumen microbiota modulation during the pre-weaning period has been suggested as means to affect animal performance later in life. In this follow-up study, we examined the post-weaning rumen microbiota development differences in monozygotic twin-heifers that were inoculated (T-group) or not inoculated (C-group) (n\uffe2\uff80\uff89=\uffe2\uff80\uff894 each) with fresh adult rumen liquid during their pre-weaning period. We also assessed the treatment effect on production parameters and methane emissions of cows during their 1st lactation period. The rumen microbiota was determined by the 16S rRNA gene, 18S rRNA gene, and ITS1 amplicon sequencing. Animal weight gain and rumen fermentation parameters were monitored from 2 to 12\uffe2\uff80\uff89months of age. The weight gain was not affected by treatment, but butyrate proportion was higher in T-group in month 3 (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.04). Apart from archaea (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.084), the richness of bacteria (p\uffe2\uff80\uff89&lt;\uffe2\uff80\uff890.0001) and ciliate protozoa increased until month 7 (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.004) and anaerobic fungi until month 11 (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.005). The microbiota structure, measured as Bray\uffe2\uff80\uff93Curtis distances, continued to develop until months 3, 6, 7, and 10, in archaea, ciliate protozoa, bacteria, and anaerobic fungi, respectively (for all: p\uffe2\uff80\uff89=\uffe2\uff80\uff890.001). Treatment or age \uffc3\uff97 treatment interaction had a significant (p\uffe2\uff80\uff89&lt;\uffe2\uff80\uff890.05) effect on 18 bacterial, 2 archaeal, and 6 ciliate protozoan taxonomic groups, with differences occurring mostly before month 4 in bacteria, and month 3 in archaea and ciliate protozoa. Treatment stimulated earlier maturation of prokaryote community in T-group before month 4 and earlier maturation of ciliate protozoa at month 2 (Random Forest: 0.75\uffe2\uff80\uff89month for bacteria and 1.5\uffe2\uff80\uff89month for protozoa). No treatment effect on the maturity of anaerobic fungi was observed. The milk production and quality, feed efficiency, and methane emissions were monitored during cow\uffe2\uff80\uff99s 1st lactation. The T-group had lower variation in energy-corrected milk yield (p\uffe2\uff80\uff89&lt;\uffe2\uff80\uff890.001), tended to differ in pattern of residual energy intake over time (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.069), and had numerically lower somatic cell count throughout their 1st lactation period (p\uffe2\uff80\uff89=\uffe2\uff80\uff890.081), but no differences between the groups in methane emissions (g/d, g/kg DMI, or g/kg milk) were observed. Our results demonstrated that the orally administered microbial inoculant induced transient changes in early rumen microbiome maturation. In addition, the treatment may influence the later production performance, although the mechanisms that mediate these effects need to be further explored.

", "keywords": ["microbiome modulation", "0301 basic medicine", "570", "ta412", "microbiome establishment", "Heifer", "dairy cow", "Rumen function", "Animal science", " dairy science", "Microbiology", "630", "Microbiome modulation", "QR1-502", "rumen function", "Microbiome establishment", "03 medical and health sciences", "Dairy cow", "heifer"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2022.983823"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2022.983823", "name": "item", "description": "10.3389/fmicb.2022.983823", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2022.983823"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-11-08T00:00:00Z"}}, {"id": "10.5194/bg-11-6969-2014", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:19:36Z", "type": "Journal Article", "created": "2014-12-11", "title": "Meta-analysis of high-latitude nitrogen-addition and warming studies implies ecological mechanisms overlooked by land models", "description": "

Abstract. Accurate representation of ecosystem processes in land models is crucial for reducing predictive uncertainty in energy and greenhouse gas feedbacks with the climate. Here we describe an observational and modeling meta-analysis approach to benchmark land models, and apply the method to the land model CLM4.5 with two versions of belowground biogeochemistry. We focused our analysis on the aboveground and belowground responses to warming and nitrogen addition in high-latitude ecosystems, and identified absent or poorly parameterized mechanisms in CLM4.5. While the two model versions predicted similar soil carbon stock trajectories following both warming and nitrogen addition, other predicted variables (e.g., belowground respiration) differed from observations in both magnitude and direction, indicating that CLM4.5 has inadequate underlying mechanisms for representing high-latitude ecosystems. On the basis of observational synthesis, we attribute the model\uffe2\uff80\uff93observation differences to missing representations of microbial dynamics, aboveground and belowground coupling, and nutrient cycling, and we use the observational meta-analysis to discuss potential approaches to improving the current models. However, we also urge caution concerning the selection of data sets and experiments for meta-analysis. For example, the concentrations of nitrogen applied in the synthesized field experiments (average = 72 kg ha\uffe2\uff88\uff921 yr\uffe2\uff88\uff921) are many times higher than projected soil nitrogen concentrations (from nitrogen deposition and release during mineralization), which precludes a rigorous evaluation of the model responses to likely nitrogen perturbations. Overall, we demonstrate that elucidating ecological mechanisms via meta-analysis can identify deficiencies in ecosystem models and empirical experiments.

", "keywords": ["0301 basic medicine", "QE1-996.5", "Ecology", "Geology", "04 agricultural and veterinary sciences", "15. Life on land", "01 natural sciences", "03 medical and health sciences", "Life", "13. Climate action", "QH501-531", "0401 agriculture", " forestry", " and fisheries", "QH540-549.5", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.5194/bg-11-6969-2014"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biogeosciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5194/bg-11-6969-2014", "name": "item", "description": "10.5194/bg-11-6969-2014", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5194/bg-11-6969-2014"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-08-18T00:00:00Z"}}, {"id": "11245.1/e982467a-6b87-4f88-8ac3-53d0fb37aeb2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:21:53Z", "type": "Journal Article", "created": "2024-05-01", "title": "Arabinosylation of cell wall extensin is required for the directional response to salinity in roots", "description": "Abstract

Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses to salinity, we developed and exploited a real-time salt-induced tilting assay. This assay follows root growth upon both gravitropic and salt challenges, revealing that root bending upon tilting is modulated by Na+ ions, but not by osmotic stress. Next, we measured this salt-specific response in 345 natural Arabidopsis (Arabidopsis thaliana) accessions and discovered a genetic locus, encoding the cell wall-modifying enzyme EXTENSIN ARABINOSE DEFICIENT TRANSFERASE (ExAD) that is associated with root bending in the presence of NaCl (hereafter salt). Extensins are a class of structural cell wall glycoproteins known as hydroxyproline (Hyp)-rich glycoproteins, which are posttranslationally modified by O-glycosylation, mostly involving Hyp-arabinosylation. We show that salt-induced ExAD-dependent Hyp-arabinosylation influences root bending responses and cell wall thickness. Roots of exad1 mutant seedlings, which lack Hyp-arabinosylation of extensin, displayed increased thickness of root epidermal cell walls and greater cell wall porosity. They also showed altered gravitropic root bending in salt conditions and a reduced salt-avoidance response. Our results suggest that extensin modification via Hyp-arabinosylation is a unique salt-specific cellular process required for the directional response of roots exposed to salinity.Drylands (hyperarid, arid, semiarid, and dry subhumid ecosystems) cover almost half of Earth\uffe2\uff80\uff99s land surface and are highly vulnerable to environmental pressures. Here we provide an inventory of soil properties including carbon (C), nitrogen (N), and phosphorus (P) stocks within the current boundaries of drylands, aimed at serving as a benchmark in the face of future challenges including increased population, food security, desertification, and climate change. Aridity limits plant production and results in poorly developed soils, with coarse texture, low C:N and C:P, scarce organic matter, and high vulnerability to erosion. Dryland soils store 646 Pg of organic C to 2\uffe2\uff80\uff89m, the equivalent of 32% of the global soil organic C pool. The magnitude of the historic loss of C from dryland soils due to human land use and cover change and their typically low C:N and C:P suggest high potential to build up soil organic matter, but coarse soil textures may limit protection and stabilization processes. Restoring, preserving, and increasing soil organic matter in drylands may help slow down rising levels of atmospheric carbon dioxide by sequestering C, and is strongly needed to enhance food security and reduce the risk of land degradation and desertification.Theory suggests that the diversity of molecules in soil organic matter (SOM functional diversity) provides key insights on multiple ecosystem services. We aimed to investigate how and why SOM functional diversity and composition change as topsoils develop, and its implications for key soil functions (e.g., from nutrient pool to water regulation).

Location

We reported data on 16 soil chronosequences globally distributed in nine countries from six continents.

Time Period

2016\uffe2\uff80\uff932017.

Major Taxa Studied

Soil microbes (bacteria and fungi) and vascular plants.

Methods

SOM functional diversity and composition without mineral interference were measured using diffuse reflectance mid\uffe2\uff80\uff90infrared Fourier transform spectroscopy (DRIFT). We aimed to characterize the main environmental factors related to SOM functional diversity and composition. Also, we calculated the links among SOM functional diversity and key soil functions.

Results

We found that SOM functional diversity declines after millions of years of soil formation (pedogenesis). We further showed that increases in plant cover and productivity led to a higher ratio of reduced (e.g., alkanes) over oxidized carbon forms (i.e., C: O\uffe2\uff80\uff90functional groups ratio), which was positively correlated to SOM functional diversity as soils age. Our findings indicated that the plant footprint (i.e., the accumulation of plant\uffe2\uff80\uff90derived material promoting the C: O\uffe2\uff80\uff90functional group ratio) would explain the reduction of SOM functional diversity as ecosystems develop. Moreover, the dissimilarity in SOM composition consistently increased with soil age, with the soil development stage emerging as the main predictor of SOM dissimilarity across contrasting biomes.

Main Conclusions

Our global survey contextualized the natural history of SOM functional diversity and composition during long\uffe2\uff80\uff90term soil development. Together, we showed how plant footprint drives the losses of SOM functional diversity with increasing age, which might provide a novel mechanism to explain typically reported losses in ecosystem functions during ecosystem retrogression.Gypsum soils in the Mediterranean Basin house large numbers of edaphic specialists that are adapted to stressful environments. The evolutionary history and standing genetic variation of these taxa have been influenced by the geological and paleoclimatic complexity of this area and the long\uffe2\uff80\uff90standing effect of human activities. However, little is known about the origin of Mediterranean gypsophiles and the factors affecting their genetic diversity and population structure.

METHODS

Using phylogenetic and phylogeographic approaches based on microsatellites and sequence data from nuclear and chloroplast regions, we evaluated the divergence time, genetic diversity, and population structure of 27 different populations of the widespread Iberian gypsophile Lepidium subulatum throughout its entire geographic range.

RESULTS

Lepidium subulatum diverged from its nearest relatives ~3 million years ago, and ITS and psbA/matK trees supported the monophyly of the species. These results suggest that both geological and climatic changes in the region around the Plio\uffe2\uff80\uff90Pleistocene promoted its origin, compared to other evolutionary processes. We found high genetic diversity in both nuclear and chloroplast markers, but a greater population structure in the chloroplast data. These results suggest that while seed dispersal is limited, pollen flow may be favored by the presence of numerous habitat patches that enhance the movement of pollinators.

CONCLUSIONS

Despite being an edaphic endemic, L. subulatum possesses high genetic diversity probably related to its relatively old age and high population sizes across its range. Our study highlights the value of using different markers to fully understand the phylogeographic history of plant species.Anthropogenic pressures on tropical forests are rapidly intensifying, but our understanding of their implications for biological diversity is still very limited, especially with regard to soil biota, and in particular soil bacterial communities. Here we evaluated bacterial community composition and diversity across a gradient of land use intensity in the eastern Amazon from undisturbed primary forest, through primary forests varyingly disturbed by fire, regenerating secondary forest, pasture, and mechanized agriculture. Soil bacteria were assessed by paired\uffe2\uff80\uff90end Illumina sequencing of 16S rRNA gene fragments (V4 region). The resulting sequences were clustered into operational taxonomic units (OTU) at a 97% similarity threshold. Land use intensification increased the observed bacterial diversity (both OTU richness and community heterogeneity across space) and this effect was strongly associated with changes in soil pH. Moreover, land use intensification and subsequent changes in soil fertility, especially pH, altered the bacterial community composition, with pastures and areas of mechanized agriculture displaying the most contrasting communities in relation to undisturbed primary forest. Together, these results indicate that tropical forest conversion impacts soil bacteria not through loss of diversity, as previously thought, but mainly by imposing marked shifts on bacterial community composition, with unknown yet potentially important implications for ecological functions and services performed by these communities.

", "keywords": ["Rios de composi\u00e7\u00e3o de comunidade bacteriana", "2. Zero hunger", "0301 basic medicine", "570", "0303 health sciences", "550", "Bacteria", "Biodiversidade subterr\u00e2nea", "Agriculture", "Biodiversity", "Forests", "15. Life on land", "Below\u2010ground biodiversity", "High\u2010throughput sequencing", "Soil", "03 medical and health sciences", "RNA", " Ribosomal", " 16S", "Sequenciamento de alto rendimento", "Rivers of bacterial community composition", "Soil Microbiology"]}, "links": [{"href": "https://eprints.lancs.ac.uk/id/eprint/82660/1/de_Carvalho_et_al_2016_raw_pdf.pdf"}, {"href": "https://doi.org/10.1002/ecy.1513"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ecy.1513", "name": "item", "description": "10.1002/ecy.1513", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ecy.1513"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-09T00:00:00Z"}}, {"id": "10.1007/s13165-020-00330-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:14:54Z", "type": "Journal Article", "created": "2020-10-07", "title": "Current use of copper, mineral oils and sulphur for plant protection in organic horticultural crops across 10 European countries", "description": "The use of several plant protection inputs of mineral origin, such as copper, sulphur or mineral oils is seen as contentious by many consumers and stakeholders within the organic sector. Although the use of these inputs is legal in organic systems and also applied in non-organic agriculture, their use by organic growers raises questions for organic practice, which aspires to be free from toxic, non-renewable chemicals. Data on the current use of permitted plant protection inputs is currently scarce, especially in horticulture where chemical inputs deserve special attention since horticultural products are often readily edible. A mapping of the use of copper, sulphur and mineral oils was conducted by collecting expert knowledge across 10 European countries during May\u2013October 2018, i.e. before the limitation of copper use to 4\u00a0kg\u00a0ha\u22121\u00a0year\u22121 from February 1, 2019. Results show that copper is widely used by Mediterranean organic growers in citrus, olive, tomato and potato production. The annual limit of 6\u00a0kg\u00a0ha\u22121\u00a0year\u22121 was not always respected. We also found that tomato producers apply high amounts of copper in winter crops in greenhouses. Mineral oils are applied to control scales, mites and whiteflies. Sulphur is also commonly used by organic vegetable growers, especially in greenhouses. We conclude that the high usage found in various different crops (especially Mediterranean crops) confirms the need for researching alternatives.", "keywords": ["[SDV.SA]Life Sciences [q-bio]/Agricultural sciences", "0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "plant protection", "571", "Crop health", " quality", " protection", "Mediterranean crops", "Greenhouse crops", "tomato", "15. Life on land", "01 natural sciences", "[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy", "Organic-PLUSX", "03 medical and health sciences", "13. Climate action", "Contentious inputs", "Vegetables", "FiBL60073", "Mediterranean crops", " Greenhouse crops", " Tomato", " Contentious inputs", " plant protection", "Abacus"]}, "links": [{"href": "https://www.iris.unict.it/bitstream/20.500.11769/494877/1/Katsoulas2020_Article_CurrentUseOfCopperMineralOilsA%20%282%29.pdf"}, {"href": "https://link.springer.com/content/pdf/10.1007/s13165-020-00330-2.pdf"}, {"href": "https://doi.org/10.1007/s13165-020-00330-2"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Organic%20Agriculture", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s13165-020-00330-2", "name": "item", "description": "10.1007/s13165-020-00330-2", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s13165-020-00330-2"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-10-07T00:00:00Z"}}, {"id": "10.1016/j.cej.2022.135748", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:19Z", "type": "Journal Article", "created": "2022-03-19", "title": "Evaluating bioelectrochemically-assisted constructed wetland (METland\u00ae) for treating wastewater: Analysis of materials, performance and electroactive communities", "description": "METland\u00ae technology consists of a bioengineering strategy for treating wastewater by integrating microbial electrochemical concepts into constructed wetland systems to enhance pollutants removal. In this context, we have constructed planted (Iris sibirica) biofilters to assess the impact of different electrically conductive bed materials (electroconductive coke, electroconductive biochar, non-electroconductive biochar and gravel) by analyzing the (i) wastewater treatment efficiency (COD and nitrogen removal), (ii) bioelectrochemical response, and (iii) diversity of microbial communities. Electrically conductive materials outperformed non-conductive ones allowing removal rates as high as 175-180 gCOD/bed*m3 day capable to support footprint as low 0.4 m2/pe. In contrast, the highest nitrogen removal rates were achieved with non-conductive biochar in presence of plants (80 %) regardless the anoxic conditions of the assay. This was confirmed by the presence of annamox bacteria like Planctomycetes. Furthermore, the presence of a marked electric potential profile along the bed height in electroconductive materials together with redox pairs (cyclic voltammetry analysis) demonstrated an effective electron flow from bottom to uppermost layers of the bed (geoconductor mechanism). In electroconductive biochar, such effective conductivity-based model co-exists with a geobattery mechanism due to presence of electroactive phenolic and carbonyl/quinone groups and/or microporosity. Microbial biodiversity analysis revealed the impact of plants just at the upper layers of the biofilters where roots and Rhizobium predominate. Bacteria from genus Clostridium were dominant in gravel inert material; in contrast, bacteria from genus Geobacter (12%) and Trichococcus (30%) outcompete the rest of communities for an effective colonization of carbonaceous beds, suggesting their main role as part of the electrosyntrophies mechanism after METland\u00ae.", "keywords": ["Electroactive bacteria", "0301 basic medicine", "0303 health sciences", "Metland\u00ae", "Electroconductive biochar", "Wastewater treatment", "QD Chemistry", "6. Clean water", "Constructed wetland", "03 medical and health sciences", "13. Climate action", "Microbial electrochemistry", "TD Environmental technology. Sanitary engineering", "Qu\u00edmica F\u00edsica"]}, "links": [{"href": "https://doi.org/10.1016/j.cej.2022.135748"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Chemical%20Engineering%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.cej.2022.135748", "name": "item", "description": "10.1016/j.cej.2022.135748", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.cej.2022.135748"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-07-01T00:00:00Z"}}, {"id": "10.1016/j.ecoenv.2020.111380", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:22Z", "type": "Journal Article", "created": "2020-10-01", "title": "Effect of the growth medium composition on nitrate accumulation in the novel protein crop Lemna minor", "description": "Duckweed is a potential alternative protein source for food and feed. However, little is known about the nitrate accumulation in this plant. A high nitrate level in vegetables can indirectly lead to an elevated intake of nitrites and N-nitroso compounds, increasing the risk of diseases for humans and animals. This research hypothesizes that the nitrate accumulation of Lemna minor differs between growing media. Additionally, it evaluates whether legal safety levels of nitrate for human and animal intake are exceeded. The duckweed was grown on (i) rainwater, and (ii) three synthetic media containing different nutrient levels. Furthermore, (iii) biological effluent of swine manure treatment and (iv) aquaculture effluent from pikeperch production were used, as these are potential media for closing nutrient loops in the agriculture sector. It was found that nitrate levels increased with the increasing availability of macronutrients in the water, and pH showed a particularly strong negative correlation with the nitrate levels in the plant. Nevertheless, nitrate content never exceeded 530\u00a0mg NO3 kg-1 fresh weight. To conclude, Lemna minor's nitrate content was below safety limits for human consumption in all tested growing media; however, a potential risk for ruminants was observed as these are more sensitive to nitrate conversions in their gastro-intestinal track.", "keywords": ["Agriculture and Food Sciences", "0106 biological sciences", "0301 basic medicine", "SAMPLES", "Wastewater", "NUTRIENTS", "01 natural sciences", "Food safety", "BIOMASS", "03 medical and health sciences", "Crude protein", "Vegetables", "Lemnaceae", "Animals", "Araceae", "Humans", "Toxicology and Mutagenesis", "PLANT", "FRUITS", "VEGETABLES", "2. Zero hunger", "Nitrates", "Environmental and Occupational Health", "Agricultural effluents", "NITRITE CONTENT", "Agriculture", "General Medicine", "Hydrogen-Ion Concentration", "Pollution", "DUCKWEEDS LEMNACEAE", "6. Clean water", "Culture Media", "NITROGEN", "Feed safety", "Health", "Public Health", "Dietary Proteins"]}, "links": [{"href": "https://doi.org/10.1016/j.ecoenv.2020.111380"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecotoxicology%20and%20Environmental%20Safety", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ecoenv.2020.111380", "name": "item", "description": "10.1016/j.ecoenv.2020.111380", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ecoenv.2020.111380"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-01T00:00:00Z"}}, {"id": "10.1029/2019jb017599", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:16:41Z", "type": "Journal Article", "created": "2019-10-31", "title": "Reply to Comment by Jennings et al. (2019b) on \u201cInvestigating Earth's Formation History through Copper and Sulfur Metal\u2010Silicate Partitioning During Core\u2010Mantle Differentiation\u201d, by Mahan et al. (2018)", "description": "Abstract

Recognizing existing materials that can act as proxies for Earth's building blocks, and understanding the accretionary pathway taken during Earth's growth, persist as outstanding issues in need of resolution. In Mahan, Siebert, Blanchard, Badro, et al. (2018, https://doi.org/10.1029/2018JB015991), we conducted diamond anvil cell (DAC) Cu metal\uffe2\uff80\uff90silicate partitioning experiments and coupled these results with a large complement of literature data to characterize Cu metal\uffe2\uff80\uff90silicate partitioning during Earth's core formation and accretion history. The Comment of Jennings, Wade, and Llovet (2019, https://doi.org/10.1029/2018JB016930) contends that secondary X\uffe2\uff80\uff90ray fluorescence, originating from the Cu holders that experiments are routinely welded to (\uffe2\uff80\uff9clift\uffe2\uff80\uff90out\uffe2\uff80\uff9d grids), compromises the novel Cu partitioning data of Mahan, Siebert, Blanchard, Badro, et al. (2018) beyond utility. To dispel these concerns and further validate our data, we have (i) investigated secondary X\uffe2\uff80\uff90ray fluorescence effects in a Cu\uffe2\uff80\uff90free experiment and provided a matrix\uffe2\uff80\uff90matched data correction, and (ii) rewelded a DAC experiment from a Cu grid to a Mo grid for a comparison of compositional analyses and Cu partitioning results. Secondary fluorescence results, in fact much like the simulated results in Jennings, Wade, and Llovet (2019), indicate that this effect is essentially equal in the metal and silicate phases and therefore has no actual impact on Cu metal\uffe2\uff80\uff90silicate partition coefficients. Moreover, Cu concentrations and partition coefficients determined using the Mo grid are statistically indistinguishable from that determined using the Cu grid. All results decisively illustrate that while secondary X\uffe2\uff80\uff90ray fluorescence must be considered where absolute concentrations are the final objective, it has had no meaningful impact on the partitioning data and observations of Mahan, Siebert, Blanchard, Badro, et al. (2018).

", "keywords": ["[SDU] Sciences of the Universe [physics]", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "550", "diamond anvil cell", "13. Climate action", "copper", "metal-silicate partitioning", "modeling", "core formation", "geochemistry"]}, "links": [{"href": "https://agupubs.onlinelibrary.wiley.com/doi/pdf/10.1029/2019JB017599"}, {"href": "https://doi.org/10.1029/2019jb017599"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Geophysical%20Research%3A%20Solid%20Earth", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1029/2019jb017599", "name": "item", "description": "10.1029/2019jb017599", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1029/2019jb017599"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-12-01T00:00:00Z"}}, {"id": "10.1016/j.gca.2019.02.036", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:39Z", "type": "Journal Article", "created": "2019-02-27", "title": "Volatile distributions in and on the Moon revealed by Cu and Fe isotopes in the \u2018Rusty Rock\u2019 66095", "description": "Abstract The Apollo 16 \u2018Rusty Rock\u2019 impact melt breccia 66095 is a volatile-rich sample, with the volatiles inherited through vapor condensation from an internal lunar source formed during thermo-magmatic evolution of the Moon. We report Cu and Fe isotope data for 66095 and find that bulk-rocks, residues and acid leaches span a relatively limited range of compositions (3.0\u202f\u00b1\u202f1.3\u202fwt.% FeO [range\u202f=\u202f2.0\u20134.8\u202fwt.%], 5.4\u202f\u00b1\u202f3.1\u202fppm Cu [range\u202f=\u202f3\u201312\u202fppm], average \u03b456Fe of 0.15\u202f\u00b1\u202f0.05\u2030 [weighted mean\u202f=\u202f0.156\u2030] and \u03b465Cu of 0.72\u202f\u00b1\u202f0.14\u2030 [weighted mean\u202f=\u202f0.78\u2030]). In contrast to the extreme enrichment of the light isotopes of Zn and heavy isotopes of Cl in 66095, \u03b465Cu and \u03b456Fe in the sample lie within the previously reported range for lunar mare basalts (0.92\u202f\u00b1\u202f0.16\u2030 and 0.12\u202f\u00b1\u202f0.02\u2030, respectively). The lack of extreme isotopic fractionation for Cu and Fe isotopes reflects compositions inherent to 66095, with condensation of a cooling gas from impact-generated fumarolic activity at temperatures too low to lead to the condensation of Cu and Fe in the sample, but higher than required to condense Zn. Together with thermodynamic models, these constraints suggest that the gas condensed within 66095 between 700 and 900\u202f\u00b0C (assuming a pressure of 10\u22126 and an fO2 of IW-2). That the Cu and Fe isotopic compositions of sample 66095 are within the range of mare basalts removes the need for an exotic, volatile-enriched source. The enrichment in Tl, Br, Cd, Sn, Zn, Pb, Rb, Cs, Ga, B, Cl, Li relative to Bi, Se, Te, Ge, Cu, Ag, Sb, Mn, P, Cr and Fe in the \u2018Rusty Rock\u2019 is consistent with volcanic outgassing models and indicates that 66095 likely formed distal from the original source of the gas. The volatile-rich character of 66095 is consistent with impact-generated fumarolic activity in the region of the Cayley Plains, demonstrating that volatile-rich rocks can occur on the lunar surface from outgassing of a volatile-poor lunar interior. The \u2018Rusty Rock\u2019 indicates that the lunar interior is significantly depleted in volatile elements and compounds and that volatile-rich surface rocks likely formed through vapor condensation. Remote sensing studies have detected volatiles on the lunar surface, attributing them dominantly to solar wind. Based on the \u2018Rusty Rock\u2019, some of these surface volatiles may also originate from the Moon\u2019s interior.", "keywords": ["[SDU] Sciences of the Universe [physics]", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "Condensates", "Copper isotopes", "13. Climate action", "Evaporation", "Iron isotopes", "Volatile elements", "Moon", "Rusty Rock"]}, "links": [{"href": "https://doi.org/10.1016/j.gca.2019.02.036"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Geochimica%20et%20Cosmochimica%20Acta", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.gca.2019.02.036", "name": "item", "description": "10.1016/j.gca.2019.02.036", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.gca.2019.02.036"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-12-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2016.09.001", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-09-20T16:16:00Z", "type": "Journal Article", "created": "2016-09-09", "title": "Temperature Sensitivity Of Soil Respiration: Synthetic Effects Of Nitrogen And Phosphorus Fertilization On Chinese Loess Plateau", "description": "Nitrogen (N) and phosphorus (P) fertilization has the potential to alter soil respiration temperature sensitivity (Q10) by changing soil biochemical and crop physiological process. A four-year field experiment was conducted to determine how Q10 responded to these biochemical and physiological changes in rain-fed agro-ecosystems on the semi-arid Loess Plateau. Soil respiration, as well as biotic and abiotic factors were measured in winter wheat (Triticum aestivum L.), with three fertilization treatments: (no fertilization (CK), 160kgNhm-1 (N), and 160kgNha-1 with 39kgPha-1 (N+P). Mean annual soil respiration rate (calculated by averaging the four years) in the N treatment and N+P treatment was 18% and 48% higher than that in the CK treatment, respectively; and it was increased by 26% (14%-48%) in the N+P treatment as compared with that in the N treatment. The decrease of Q10 in the N and N+P treatments against the CK treatment was not stable for each year, ranging from 0.01 to 0.28. The maximum decrease of Q10 in the N and N+P treatments was 10% and 15% in 2014-2015, while in other years the decrease of Q10 was numerical but not significant. Soil microbial biomass carbon (SMBC) was increased by 10% and 50%, dissolved organic carbon (DOC) was increased by 6% and 21%, and photosynthesis rate was increased ranging from 6% to 33% with N and N+P fertilization. The relative abundance of Acidobacteria, Actinobacteria and Chloroflexi were significantly higher by 32.9%-54.1% in N addition soils (N and N+P) compared to CK treatment, whereas additional P application into soils increased the relative abundance of the family Micrococcaceae, Nocardioidaceae and Chitinophagaceae. Soil respiration was positively related to SMBC, DOC and photosynthesis rate (p<0.05). However, variation in Q10 may be related to the increase of soil mineral N content and variation of the relative abundance of soil microbial community in our study. Nitrogen and additional phosphorus fertilization regimes affect soil respiration and temperature sensitivity differently.", "keywords": ["0301 basic medicine", "2. Zero hunger", "03 medical and health sciences", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "6. Clean water"], "contacts": [{"organization": "Qingfang Liu, Rui Wang, Shengli Guo, Shengli Guo, Ying Wang, Qiqi Sun, Man Zhao, Yaxian Hu, Xin Gao, Lanlan Du,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2016.09.001"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2016.09.001", "name": "item", "description": "10.1016/j.scitotenv.2016.09.001", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2016.09.001"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-01-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2018.08.257", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:16:00Z", "type": "Journal Article", "created": "2018-08-23", "title": "Assessment of promising agricultural management practices", "description": "iSQAPER project - Interactive Soil Quality Assessment in Europe and China for Agricultural Productivity and Environmental Resilience - aims to develop an app to advise farmers on selecting the best Agriculture Management Practice (AMPs) to improve soil quality. For this purpose, a soil quality index has to be developed to account for the changes in soil quality as impacted by the implementation of the AMPs. Some promising AMPs have been suggested over the time to prevent soil degradation. These practices have been randomly adopted by farmers but which practices are most used by farmers and where they are mostly adopted remains unclear. This study is part of the iSQAPER project with the specific aims: 1) map the current distribution of previously selected 18 promising AMPs in several pedo-climatic regions and farming systems located in ten and four study site areas (SSA) along Europe and China, respectively; and 2) identify the soil threats occurring in those areas. In each SSA, farmers using promising AMP's were identified and questionnaires were used to assess farmer's perception on soil threats significance in the area. 138 plots/farms using 18 promising AMPs, were identified in Europe (112) and China (26).Results show that promising AMPs used in Europe are Crop rotation (15%), Manuring & Composting (15%) and Min-till (14%), whereas in China are Manuring & Composting (18%), Residue maintenance (18%) and Integrated pest and disease management (12%). In Europe, soil erosion is the main threat in agricultural Mediterranean areas while soil-borne pests and diseases is more frequent in the SSAs from France and The Netherlands. In China, soil erosion, SOM decline, compaction and poor soil structure are among the most significant. This work provides important information for policy makers and the development of strategies to support and promote agricultural management practices with benefits for soil quality.", "keywords": ["0301 basic medicine", "2. Zero hunger", "S1 Agriculture (General) / mez\u0151gazdas\u00e1g \u00e1ltal\u00e1ban", "04 agricultural and veterinary sciences", "Environment", "15. Life on land", "03 medical and health sciences", "Sustainability", "13. Climate action", "Farming systems; Sustainability; Soil threats; Environment", "Farming systems", "Soil threats", "0401 agriculture", " forestry", " and fisheries", "504 - Ciencias del medio ambiente"]}, "links": [{"href": "https://doi.org/10.1016/j.scitotenv.2018.08.257"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2018.08.257", "name": "item", "description": "10.1016/j.scitotenv.2018.08.257", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2018.08.257"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-02-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2012.04.004", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:16:09Z", "type": "Journal Article", "created": "2012-04-23", "title": "Biochar But Not Earthworms Enhances Rice Growth Through Increased Protein Turnover", "description": "The aim of this work was to compare the effects of biochar and earthworms on rice growth and to investigate the possible interactions between both. In addition to classic macroscopic variables we also monitored some leaf-level cellular processes involved in protein turnover. Both biochar and earthworms significantly increased shoot biomass production. However, biochar had a higher effect on the number of leaves (\u00fe87%) and earthworms on leaf area (\u00fe89%). Biochar also significantly increased the leaf turnover. At the cellular level, biochar but not earthworms enhanced protein catabolism by an increase in leaf proteolytic activities. This could be related to the increased expression of three of the six genes tested related to protein catabolism, one serine protease gene OsSP2 (\u00fe24%), one aspartic acid protease gene, Oryzasin (\u00fe162%) and one cysteine protease gene OsCatB (\u00fe257%). Furthermore, biochar also enhanced the expression level of two genes linked to protein anabolism, coding for the small and large subunits of rubisco (\u00fe33% and \u00fe30%, for rbcS and rbcL, respectively), the most abundant protein in leaves. In conclusion, our data gives evidence that biochar increased rice biomass production through increased leaf protein turnover (both catabolism and anabolism) whereas earthworms also increased rice biomass production but not through changes in the rate of protein turnover. We hypothesize that earthworms increase nitrogen uptake at a low cost for the plant through a simultaneous increase in mineralization rate and root biomass, probably through the release in the soil of plant growth factors. This could allow plants to accumulate more biomass without an increase in nitrogen metabolism at the leaf level, and without having to support the consecutive energy cost that must bear plants in the biochar treatment. 2012 Elsevier Ltd. All rights reserved.", "keywords": ["0106 biological sciences", "0301 basic medicine", "earthworms", "FAUNE DU SOL", "7. Clean energy", "01 natural sciences", "03 medical and health sciences", "AZOTE", "PROTEINE", "CROISSANCE", "ETUDE COMPARATIVE", "lombriz de tierra", "2. Zero hunger", "BIOMASSE", "BIOCHAR", "biomass", "carbon", "microbiology", "MACROFAUNE", "CHARBON DE BOIS", "carbono", "RIZICULTURE", "biomasa", "oryza sativa", "METABOLISME", "FERTILISATION DU SOL", "carb\u00f3n vegetal", "LOMBRIC", "FEUILLE", "charcoal"], "contacts": [{"organization": "Kam-Rigne Laossi, Juan Andr\u00e9s Cardoso, Patrick Lavelle, Patrick Lavelle, Diana Cristina Noguera, Diana Cristina Noguera, Diana Cristina Noguera, M.H. Cruz de Carvalho, S\u00e9bastien Barot,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2012.04.004"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2012.04.004", "name": "item", "description": "10.1016/j.soilbio.2012.04.004", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2012.04.004"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-09-01T00:00:00Z"}}, {"id": "10.1016/j.agee.2017.06.003", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:04Z", "type": "Journal Article", "created": "2017-06-10", "title": "Effects Of Shade-Tree Species And Spacing On Soil And Leaf Nutrient Concentrations In Cocoa Plantations At 8 Years After Establishment", "description": "Intercropping in agroforestry systems improves ecosystem services. Appropriate species compositions and spacing regimes are critical to achieve ecosystem benefits and improve yields of all the component crops. Cocoa (Theobroma cacao) is an important cash crop globally but it requires shade for survival and growth. However, the effects of shade-tree species composition and spacing regime on nutrient cycling in cocoa plantations are not well understood. This study investigated the effects of shade tree species and spacing regimes on soil and plant nutrient availability at 8 years after plantation establishment in Papua New Guinea. Three cocoa intercropping systems were established in which T. cacao was planted with either a non-legume timber tree, Canarium indicum, or a legume non-timber tree, Gliricidia sepium. The shade-tree spacing regimes included either 8 m \u00d7 16 m or 8 m \u00d7 8 m in the Theobroma + Canarium plantations. There was an ongoing thinning regime in the Theobroma + Gliricidia plantation, with a final shade-tree spacing of 12 m \u00d7 12 m. Soil total carbon (TC) and total nitrogen (TN) were significantly higher in the Theobroma + Gliricidia plantation with 12 m \u00d7 12 m spacing and the Theobroma + Canarium plantation with 8 m \u00d7 16 m spacing than in the Theobroma + Canarium plantation with 8 m \u00d7 8 m spacing. Foliar TN and P were correlated with soil TN and P, respectively, whereas no correlation was detected between soil and leaf K concentrations. Foliar TN, P and K were under ideal concentrations for T. cacao in all of the plantations. The Theobroma + Gliricidia plantation had higher soil water extractable phosphorus (P) than the two Theobroma + Canarium plantations, probably due to frequent pruning of the G. sepium trees. Foliar C isotope composition (\u03b413C) of T. cacao suggested that T. cacao close to G. sepium or close to C. indicum with spacing of 8 m \u00d7 16 m and 8 m \u00d7 8 m had similar light interception. However, increased C. indicum spacing increased the light interception of T. cacao trees that were not planted next to C. indicum. This study indicated that non-legume timber trees with an optimized spacing regime can be used as overstorey shade trees for T. cacao. However, our study indicated all three plantations required fertilisation and better nutrient management.", "keywords": ["571", "stable isotopes", "FoR 16 (Studies in Human Society)", "Canarium indicum", "Soil fertility", "Gliricidia sepium", "333", "630", "Papua New Guinea", "veterinary and food sciences", "Stable isotopes", "2. Zero hunger", "Field organic and low chemical input horticulture", "Agricultural", "Science & Technology", "Multidisciplinary", "Ecology", "soil fertility", "FoR 07 (Agricultural and Veterinary Sciences)", "Human society", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Environmental sciences", "260516 Tropical fruit", "gliricidia sepium", "Intercropping", "070501 Agroforestry", "0401 agriculture", " forestry", " and fisheries", "FoR 05 (Environmental Sciences)", "intercropping", "Life Sciences & Biomedicine"]}, "links": [{"href": "https://doi.org/10.1016/j.agee.2017.06.003"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2017.06.003", "name": "item", "description": "10.1016/j.agee.2017.06.003", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2017.06.003"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-08-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2018.09.128", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:29Z", "type": "Journal Article", "created": "2018-09-28", "title": "A rationale for the high limits of quantification of antibiotic resistance genes in soil", "description": "The determination of values of abundance of antibiotic resistance genes (ARGs) per mass of soil is extremely useful to assess the potential impacts of relevant sources of antibiotic resistance, such as irrigation with treated wastewater or manure application. Culture-independent methods and, in particular, quantitative PCR (qPCR), have been regarded as suitable approaches for such a purpose. However, it is arguable if these methods are sensitive enough to measure ARGs abundance at levels that may represent a risk for environmental and human health. This study aimed at demonstrating the range of values of ARGs quantification that can be expected based on currently used procedures of DNA extraction and qPCR analyses. The demonstration was based on the use of soil samples spiked with known amounts of wastewater antibiotic resistant bacteria (ARB) (Enterococcus faecalis, Escherichia coli, Acinetobacter johnsonii, or Pseudomonas aeruginosa), harbouring known ARGs, and also on the calculation of expected values determined based on qPCR. The limits of quantification (LOQ) of the ARGs (vanA, qnrS, blaTEM, blaOXA, blaIMP, blaVIM) were observed to be approximately 4 log-units per gram of soil dry weight, irrespective of the type of soil tested. These values were close to the theoretical LOQ values calculated based on currently used DNA extraction methods and qPCR procedures. The observed LOQ values can be considered extremely high to perform an accurate assessment of the impacts of ARGs discharges in soils. A key message is that ARGs accumulation will be noticeable only at very high doses. The assessment of the impacts of ARGs discharges in soils, of associated risks of propagation and potential transmission to humans, must take into consideration this type of evidence, and avoid the simplistic assumption that no detection corresponds to risk absence.", "keywords": ["0301 basic medicine", "2. Zero hunger", "LOD - Limit of detection", "0303 health sciences", "Acinetobacter", "Drug Resistance", " Microbial", "Wastewater", "Real-Time Polymerase Chain Reaction", "6. Clean water", "Anti-Bacterial Agents", "3. Good health", "Manure", "Quantitative PCR", "Soil", "03 medical and health sciences", "Genes", " Bacterial", "13. Climate action", "Pseudomonas aeruginosa", "Enterococcus faecalis", "Escherichia coli", "LOQ - Limit of quantification", "Soil Microbiology", "Risk assessment"]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2018.09.128"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2018.09.128", "name": "item", "description": "10.1016/j.envpol.2018.09.128", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2018.09.128"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-12-01T00:00:00Z"}}, {"id": "10.1016/j.envpol.2024.125307", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:30Z", "type": "Journal Article", "created": "2024-11-12", "title": "Biodegradable microplastics induce profound changes in lettuce (Lactuca sativa) defense mechanisms and to some extent deteriorate growth traits", "description": "The development of agricultural technologies has intensified the use of plastic in this sector. Products of plastic degradation, such as microplastics (MPs), potentially threaten living organisms, biodiversity and agricultural ecosystem functioning. Thus, biodegradable plastic materials have been introduced to agriculture. However, the effects of biodegradable plastic substitutes on soil ecosystems are even less known than those of traditional ones. Here, we studied the effects of environmentally relevant concentrations of MPs prepared from a biodegradable plastic (a starch-polybutylene adipate terephthalate blend, PBAT-BD-MPs) on the growth and defense mechanisms of lettuce (Lactuca sativa) in CLIMECS system (CLImatic Manipulation of ECosystem Samples). PBAT-BD-MPs in the highest concentrations negatively affected some traits of growth, i.e., dry weight percentage, specific leaf area, and both C and N contents. We observed more profound changes in plant physiology and biochemistry, as PBAT-BD-MPs decreased chlorophyll content and triggered a concerted response of plant defense mechanisms against oxidative stress. In conclusion, exposure to PBAT-BD-MPs induced plant oxidative stress and activated plant defense mechanisms, leading to oxidative homeostasis that sustained plant growth and functioning. Our study highlights the need for in-depth understanding of the effect of bioplastics on plants.", "keywords": ["580", "Chlorophyll", "0301 basic medicine", "570", "0303 health sciences", "salicylic acid", "Microplastics", "Lipid peroxidation", "lipid peroxidation", "Salicylic acid", "Biodegradable Plastics", "Plant Leaves", "Oxidative Stress", "03 medical and health sciences", "Starch-polybutylene adipate terephthalate", "Biodegradation", " Environmental", "total phenolic content", "starch-polybutylene adipate terephthalate", "Soil Pollutants", "PBAT", "Total phenolic content", "CLIMECS system", "Lactuca"], "contacts": [{"organization": "Sylwia Adamczyk, Laura J. Zantis, Sam van Loon, Cornelis A.M. van Gestel, Thijs Bosker, Rachel Hurley, Luca Nizzetto, Bartosz Adamczyk, Sannakajsa Velmala,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1016/j.envpol.2024.125307"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Pollution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envpol.2024.125307", "name": "item", "description": "10.1016/j.envpol.2024.125307", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envpol.2024.125307"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-01T00:00:00Z"}}, {"id": "10.1016/j.fm.2020.103583", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:15:32Z", "type": "Journal Article", "created": "2020-06-27", "title": "Combined application of antagonistic Wickerhamomyces anomalus BS91 strain and Cynara cardunculus L. leaf extracts for the control of postharvest decay of citrus fruit", "description": "Combined use of biocontrol agents and plant extracts can be considered a viable and promising strategy for protecting plant tissues with different synergistic mechanisms of action that improve the antimicrobial activity of the mixtures. Treatments of citrus fruits with Wickerhamomyces anomalus BS91 have been previously reported as effective measures to reduce the incidence of green mold caused by Penicillium digitatum. On the opposite, the knowledge of the antifungal activity of cultivated cardoon (Cynara cardunculus L. var. altilis DC.) leaf extract, vegetable widespread in some Mediterranean areas, is still very limited. In this study, experimental trials were conducted to evaluate the effectiveness in vitro of leaf aqueous, methanolic and ethanolic extracts of C. cardunculus against seven fungal pathogens responsible for considerable food losses in the postharvest stage. In addition, biocontrol yeast W. anomalus BS91 and the three C. cardunculus extracts were tested in vivo both as a single treatment and in mixture, against Penicillium digitatum on 'Tarocco' oranges and 'Femminello' lemons. The combination of W. anomalus BS91 and leaf ethanolic extract reduced with the highest efficacy the incidence and severity of green mold on orange and lemon fruits with respect to the control, and was more effective than treatment with antagonistic yeast or leaf extracts applied alone. Incidence and severity of citrus decay were more consistently reduced when mixtures were applied 24\u00a0h before the inoculation of the pathogen, thus suggesting the relevance of preventive treatments. The mixtures of antagonistic W. anomalus BS91 and ethanolic leaf extract were more effective in controlling green mold decay on oranges than on lemons. These results indicate that biocontrol agents and leaf extracts, used in appropriate combination, can provide a stronger protection than when used singularly. However, compatibility between microbial antagonist and antimicrobial extract should be preliminary verified.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Citrus", "0303 health sciences", "Plant Extracts", "Penicillium", "Biocontrol; Yeasts; Cynara cardunculus; Citrus; Green mold; Postharvest", "Cynara", "Biocontrol", " Citrus", " Cynara cardunculus", " Green mold", " Postharvest", " Yeasts", "03 medical and health sciences", "Biological Control Agents", "Food Preservation", "Fruit", "Antibiosis", "Saccharomycetales", "Biocontrol; Citrus; Cynara cardunculus; Green mold; Postharvest; Yeasts", "Plant Diseases"]}, "links": [{"href": "https://www.iris.unict.it/bitstream/20.500.11769/494574/1/Food%20Microbiology%202020.pdf"}, {"href": "https://doi.org/10.1016/j.fm.2020.103583"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Food%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.fm.2020.103583", "name": "item", "description": "10.1016/j.fm.2020.103583", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.fm.2020.103583"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-01T00:00:00Z"}}, {"id": "10.1101/2023.06.28.546105", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:17:20Z", "type": "Journal Article", "created": "2023-06-29", "title": "Construction and Characterisation of a Structured, Tuneable, and Transparent 3D Culture Platform for Soil Bacteria", "description": "2.Abstract

We have developed a tuneable workflow for the study of soil microbes in an imitative 3D soil environment that is compatible with routine and advanced optical imaging, is chemically customisable, and is reliably refractive index matched based on the metabolic profile of the study organism. We demonstrate our transparent soil pipeline with two representative soil organisms,Bacillus subtilisandStreptomyces coelicolor, and visualise their colonisation behaviours using fluorescence microscopy and mesoscopy. This spatially structured, 3D approach to microbial culture has the potential to further study the behaviour of other difficult-to-culture bacteria in conditions matching their native environment and could be expanded to study microbial interactions, such as interaction, competition, and warfare.

3.Graphical Abstract

A step-by-step method for creating a tailored 3D culture medium for study of soil microbes.

The complete workflow can be split into three parts: Growth and observation, metabolic profiling to provide a stable refractive index matching solution, and production of the 3D soil environment. The 3D culture scaffold was created by cryomilling Nafion\uffe2\uff84\uffa2 resin pellets and size filtration. Chemical processing altered the surface chemistry of Nafion\uffe2\uff84\uffa2 particles and facilitated nutrient binding by titration of a defined liquid culture medium. Metabolic profiling determined non-metabolisable sugars and provided an inert refractive index matching substrate, which was added to the final nutrient titration. Inoculation and growth of the test strain allowed for downstream assessment of colonisation behaviours and community dynamicsin situby, for example, optical microscopy.Establishing Lupinus mutabilis as a protein and oil crop requires improved varieties adapted to EU climates. The genetic regulation of strategic breeding traits, including plant architecture, growing cycle length and yield, is unknown. This study aimed to identify associations between 16 669 single nucleotide polymorphisms (SNPs) and 9 agronomic traits on a panel of 223 L. mutabilis accessions, grown in four environments, by applying a genome wide association study (GWAS). Seven environment-specific QTLs linked to vegetative yield, plant height, pods number and flowering time, were identified as major effect QTLs, being able to capture 6 to 20% of the phenotypic variation observed in these traits. Furthermore, two QTLs across environments were identified for flowering time on chromosome 8. The genes FAF, GAMYB and LNK, regulating major pathways involved in flowering and growth habit, as well as GA30X1, BIM1, Dr1, HDA15, HAT3, interacting with these pathways in response to hormonal and environmental cues, were prosed as candidate genes. These results are pivotal to accelerate the development of L. mutabilis varieties adapted to European cropping conditions by using marker-assisted selection (MAS), as well as to provide a framework for further functional studies on plant development and phenology in this species.Background: Water and nitrogen (N) are essential resources influencing plant growth and yield. To improve their efficiencies in crop production is challenging because the physiological mechanisms of water and N coupling and their interactive effect on crop water use efficiency (WUE) are not well understood yet.

Aim: The aim of this study was to investigate the physiological responses and phytohormones signaling in oats in response to soil water status and N supply under fertigation, to explore the mechanisms regulating plant growth and WUE.

Methods: Oat plants were subjected to the factorial combination of three soil moisture regimes (50, 70, and 90% of soil water holding capacity, SWHC) and three N levels (fertilized with 74, 149, and 298 mg kg\uffe2\uff88\uff921).

Results: The stomatal conductance (gs) was significantly decreased by soil water deficit, and also by the highest N level, whereas photosynthesis rate (An) was unaffected by neither water nor N. Consequently, intrinsic WUE (WUEint, An/gs) was highest under reduced irrigation and high N fertilization. This effect at stomatal level was affirmed by responses in whole plant WUE (WUEb), which was positively correlated with shoot \uffce\uffb413C. A positive correlation between \uffce\uffb418O and \uffce\uffb413C in shoots further indicated that decreases of gs rather than changes in An contributed to the enhanced WUE.

Conclusion: Moderate soil water deficit and sufficient N supply is recommended for saving irrigation water and improving WUE on fertigated oat plants without compromising biomass accumulation to any large extent.Broadband topological metamaterials hold the key for designing the next generation of integrated photonic platforms and microwave devices given their protected back\uffe2\uff80\uff90scattering\uffe2\uff80\uff90free and unidirectional edge states, among other exotic properties. However, synthesizing such metamaterial has proven challenging. Here, a broadband bandgap (relative bandwidth of more than 43%) Valley\uffe2\uff80\uff90Hall topological metamaterial with deep subwavelength thickness is proposed. The present topological metamaterial is composed of three layers printed circuit boards whose total thickness is 1.524\uffc2\uffa0mm \uffe2\uff89\uff88 \uffce\uffbb/100. The topological phase transition is achieved by introducing an asymmetry parameter \uffce\uffb4r. Three mechanically reconfigurable edge states can be obtained by varying interlayer displacement. Their robust transmission is demonstrated through two kinds of waveguide domain walls with cavities and disorders. Exploiting the proposed topological metamaterial, a six\uffe2\uff80\uff90way power divider is constructed and measured as a proof\uffe2\uff80\uff90of\uffe2\uff80\uff90concept of the potential of the proposed technology for future electromagnetic devices.Haloalkane dehalogenases (HLDs) are \uffce\uffb1/\uffce\uffb2\uffe2\uff80\uff90hydrolases that convert halogenated compounds to their corresponding alcohols. The overall kinetic mechanism proceeds via four steps: (i) binding of halogenated substrate, (ii) bimolecular nucleophilic substitution (SN2) leading to the cleavage of a carbon\uffe2\uff80\uff90halogen bond and the formation of an alkyl\uffe2\uff80\uff90enzyme intermediate, (iii) nucleophilic addition of a water molecule resulting in the hydrolysis of the intermediate to the corresponding alcohol and (iv) release of the reaction products \uffe2\uff80\uff93 an alcohol, a halide ion and a proton. Although, the overall reaction has been reported as irreversible, several kinetic evidences from previous studies suggest the reversibility of the first SN2 chemical step. To study this phenomenon, we have engineered HLDs to stop the catalytic cycle at the stage of the alkyl\uffe2\uff80\uff90enzyme intermediate. The ability of the intermediate to exchange halides was confirmed by a stopped\uffe2\uff80\uff90flow fluorescence binding analysis. Finally, the transhalogenation reaction was confirmed with several HLDs and 2,3\uffe2\uff80\uff90dichloropropene in the presence of a high concentration of iodide. The formation of the transhalogenation product 3\uffe2\uff80\uff90iodo\uffe2\uff80\uff902\uffe2\uff80\uff90chloropropene catalysed by five mutant HLDs was identified by gas chromatography coupled with mass spectrometry. Hereby we demonstrated the reversibility of the cleavage of the carbon\uffe2\uff80\uff90halogen bond by HLDs resulting in a transhalogenation. After optimization, the transhalogenation reaction can possibly find its use in biocatalytic applications. Enabling this reaction by strategically engineering the enzyme to stop at an intermediate in the catalytic cycle that is synthetically more useful than the product of the natural pathway is a novel concept.

magnified image

", "keywords": ["0301 basic medicine", "enzyme catalysis; halide exchange; haloalkane dehalogenase; nucleophilic substitution; redirection of reaction; transhalogenation", "0303 health sciences", "03 medical and health sciences"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/adsc.201900132"}, {"href": "https://doi.org/10.1002/adsc.201900132"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Advanced%20Synthesis%20%26amp%3B%20Catalysis", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/adsc.201900132", "name": "item", "description": "10.1002/adsc.201900132", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/adsc.201900132"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-17T00:00:00Z"}}, {"id": "10.1002/advs.201901408", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-20T16:14:02Z", "type": "Journal Article", "created": "2019-09-19", "title": "Natural Microbial Communities Can Be Manipulated by Artificially Constructed Biofilms", "description": "Abstract

Biofouling proceeds in successive steps where the primary colonizers affect the phylogenetic and functional structure of a future microbial consortium. Using microbiologically influenced corrosion (MIC) as a study case, a novel approach for material surface protection is described, which does not prevent biofouling, but rather shapes the process of natural biofilm development to exclude MIC\uffe2\uff80\uff90related microorganisms. This approach interferes with the early steps of natural biofilm formation affecting how the community is finally developed. It is based on a multilayer artificial biofilm, composed of electrostatically modified bacterial cells, producing antimicrobial compounds, extracellular antimicrobial polyelectrolyte matrix, and a water\uffe2\uff80\uff90proof rubber elastomer barrier. The artificial biofilm is constructed layer\uffe2\uff80\uff90by\uffe2\uff80\uff90layer (LBL) by manipulating the electrostatic interactions between microbial cells and material surfaces. Field testing on standard steel coupons exposed in the sea for more than 30 days followed by laboratory analyses using molecular\uffe2\uff80\uff90biology tools demonstrate that the preapplied artificial biofilm affects the phylogenetic structure of the developing natural biofilm, reducing phylogenetic diversity and excluding MIC\uffe2\uff80\uff90related bacteria. This sustainable solution for material protection showcases the usefulness of artificially guiding microbial evolutionary processes via the electrostatic modification and controlled delivery of bacterial cells and extracellular matrix to the exposed material surfaces.The relationship between the spatial variability of soil multifunctionality (i.e., the capacity of soils to conduct multiple functions; SVM) and major climatic drivers, such as temperature and aridity, has never been assessed globally in terrestrial ecosystems. We surveyed 236 dryland ecosystems from six continents to evaluate the relative importance of aridity and mean annual temperature, and of other abiotic (e.g., texture) and biotic (e.g., plant cover) variables as drivers of SVM, calculated as the averaged coefficient of variation for multiple soil variables linked to nutrient stocks and cycling. We found that increases in temperature and aridity were globally correlated to increases in SVM. Some of these climatic effects on SVM were direct, but others were indirectly driven through reductions in the number of vegetation patches and increases in soil sand content. The predictive capacity of our structural equation\uffc2\uffa0modelling was clearly higher for the spatial variability of N\uffe2\uff80\uff90 than for C\uffe2\uff80\uff90 and P\uffe2\uff80\uff90related soil variables. In the case of N cycling, the effects of temperature and aridity were both direct and indirect via changes in soil properties. For C and P, the effect of climate was mainly indirect via changes in plant attributes. These results suggest that future changes in climate may decouple the spatial availability of these elements for plants and microbes in dryland soils. Our findings significantly advance our understanding of the patterns and mechanisms driving SVM in drylands across the globe, which is critical for predicting changes in ecosystem functioning in response to climate change.There is an urgent need to develop viable, renewable, sustainable energy systems that can reduce global dependence on fossil fuel sources of energy. Biofuels such as ethanol are being utilized as blends in surface transportation fuels and have the potential to improve sustainability and reduce greenhouse gas emissions in the short term. Bioethanol, the most widely used liquid biofuel, is currently produced by converting sugars or starches from feed crops into ethanol. Use of this fuel source displaces and draws water consumption away from agricultural crops, increases soil erosion by shifting land from perennial grasses to annual crops, and increases use of fertilizers and insecticides. In contrast, bioethanol made from lignocellulosic biomass feedstocks does not have these limitations and in addition, offers a larger resource base: the amount of cellulosic material available for potential use vastly outweighs the amount of available starch\uffe2\uff80\uff90based feedstock. Therefore, bioethanol from lignocellulosic biomass has attracted considerable interest from biofuel developers. This review is an update of some developments to optimize cellulose extraction from feedstock crops and to improve crop yields and logistics. It concludes that agricultural and forestry systems that incorporate lignocellulosic biomass crops can be designed for improved ecological function and energy use efficiency. Development of crops that have both desirable cell\uffe2\uff80\uff90wall traits and high biomass productivity under sustainable low\uffe2\uff80\uff90input conditions can significantly enhance the economics and efficiency of the conversion process. Optimizing the logistics of moving feedstock from field or forest to bio\uffe2\uff80\uff90refinery can significantly reduce costs of using lignocellulosic feedstocks. \uffc2\uffa9 2013 Society of Chemical Industry and John Wiley & Sons, Ltd

", "keywords": ["0301 basic medicine", "2. Zero hunger", "03 medical and health sciences", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "7. Clean energy", "12. Responsible consumption"]}, "links": [{"href": "https://doi.org/10.1002/bbb.1418"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biofuels%2C%20Bioproducts%20and%20Biorefining", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/bbb.1418", "name": "item", "description": "10.1002/bbb.1418", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/bbb.1418"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-07-09T00:00:00Z"}}, {"id": "10.1002/bbb.325", "type": "Feature", "geometry": null, "properties": {"license": "Closed Access", "updated": "2026-09-20T16:14:03Z", "type": "Journal Article", "created": "2011-09-01", "title": "Biofuels: Ethics And Policy-Making", "description": "Abstract

Mandatory targets for biofuels have led to their rapid global adoption, but ethical problems with their large\uffe2\uff80\uff90scale production are widely reported. Research is underway to find new biofuel technologies that mitigate climate change and can be produced sustainably and economically. Following an 18\uffe2\uff80\uff90month inquiry, the Nuffield Council on Bioethics has developed an ethical framework for biofuels policy\uffe2\uff80\uff90making. Based on a number of widely held ethical values, six ethical principles are introduced that should be considered in biofuels policy\uffe2\uff80\uff90making. Many current biofuels policies fail this ethical \uffe2\uff80\uff98test\uffe2\uff80\uff99. An overarching ethical standard for biofuels is proposed that includes the protection of human rights and the environment, full life cycle assessment of greenhouse gas emissions, trade principles that are fair, access and benefit\uffe2\uff80\uff90sharing schemes to encourage equitable distribution of costs and benefits, and instruments to incentivize the development of promising new approaches. The Council goes on to make recommendations for changes to current policy. \uffc2\uffa9 2011 Society of Chemical Industry and John Wiley & Sons, Ltd

", "keywords": ["0301 basic medicine", "03 medical and health sciences", "13. Climate action", "01 natural sciences", "7. Clean energy", "0105 earth and related environmental sciences", "12. Responsible consumption", "3. Good health"], "contacts": [{"organization": "Joyce Tait, Alena Buyx,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1002/bbb.325"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biofuels%2C%20Bioproducts%20and%20Biorefining", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/bbb.325", "name": "item", "description": "10.1002/bbb.325", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/bbb.325"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-08-25T00:00:00Z"}}, {"id": "10.1002/biot.202000165", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:14:03Z", "type": "Journal Article", "created": "2020-10-21", "title": "Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates", "description": "Abstract

Growing environmental concern sparks renewed interest in the sustainable production of (bio)materials that can replace oil\uffe2\uff80\uff90derived goods. Polyhydroxyalkanoates (PHAs) are isotactic polymers that play a critical role in the central metabolism of producer bacteria, as they act as dynamic reservoirs of carbon and reducing equivalents. PHAs continue to attract industrial attention as a starting point toward renewable, biodegradable, biocompatible, and versatile thermoplastic and elastomeric materials. Pseudomonas species have been known for long as efficient biopolymer producers, especially for medium\uffe2\uff80\uff90chain\uffe2\uff80\uff90length PHAs. The surge of synthetic biology and metabolic engineering approaches in recent years offers the possibility of exploiting the untapped potential of Pseudomonas cell factories for the production of tailored PHAs. In this article, an overview of the metabolic and regulatory circuits that rule PHA accumulation in Pseudomonas putida is provided, and approaches leading to the biosynthesis of novel polymers (e.g., PHAs including nonbiological chemical elements in their structures) are discussed. The potential of novel PHAs to disrupt existing and future market segments is closer to realization than ever before. The review is concluded by pinpointing challenges that currently hinder the wide adoption of bio\uffe2\uff80\uff90based PHAs, and strategies toward programmable polymer biosynthesis from alternative substrates in engineered P. putida strains are proposed.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Biopolymer", "PHA", "Pseudomonas putida", "Polyhydroxyalkanoates", "Carbon", "12. Responsible consumption", "03 medical and health sciences", "/dk/atira/pure/sustainabledevelopmentgoals/affordable_and_clean_energy; name=SDG 7 - Affordable and Clean Energy", "Metabolic Engineering", "Pseudomonas", "Pathway engineering", "Metabolic engineering", "Synthetic biology"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.202000165"}, {"href": "https://doi.org/10.1002/biot.202000165"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biotechnology%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/biot.202000165", "name": "item", "description": "10.1002/biot.202000165", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/biot.202000165"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-11-09T00:00:00Z"}}, {"id": "10.1002/cbic.202000051", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:14:03Z", "type": "Journal Article", "created": "2020-01-31", "title": "An Engineered E.\u2005coli Strain for Direct in Vivo Fluorination", "description": "Abstract

Selectively fluorinated compounds are found frequently in pharmaceutical and agrochemical products where currently 25\uffe2\uff80\uff9330\uffe2\uff80\uff89% of optimised compounds emerge from development containing at least one fluorine atom. There are many methods for the site\uffe2\uff80\uff90specific introduction of fluorine, but all are chemical and they often use environmentally challenging reagents. Biochemical processes for C\uffe2\uff88\uff92F bond formation are attractive, but they are extremely rare. In this work, the fluorinase enzyme, originally identified from the actinomycete bacterium Streptomyces cattleya, is engineered into Escherichia coli in such a manner that the organism is able to produce 5\uffe2\uff80\uffb2\uffe2\uff80\uff90fluorodeoxyadenosine (5\uffe2\uff80\uffb2\uffe2\uff80\uff90FDA) from S\uffe2\uff80\uff90adenosyl\uffe2\uff80\uff90l\uffe2\uff80\uff90methionine (SAM) and fluoride in live E.\uffe2\uff80\uff85coli cells. Success required the introduction of a SAM transporter and deletion of the endogenous fluoride efflux capacity in order to generate an E.\uffe2\uff80\uff85coli host that has the potential for future engineering of more elaborate fluorometabolites.

", "keywords": ["SAM transporters", "0301 basic medicine", "570", "S-Adenosylmethionine", "0303 health sciences", "Deoxyadenosines", "Halogenation", "DAS", "Fluorine", "Halogenations", "540", "QD Chemistry", "Streptomyces", "3. Good health", "03 medical and health sciences", "Bacterial Proteins", "Isomerism", "Escherichia coli", "QD", "Fluoride channels", "Genetic Engineering", "Oxidoreductases", "Fluorinases"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/cbic.202000051"}, {"href": "https://doi.org/10.1002/cbic.202000051"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ChemBioChem", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/cbic.202000051", "name": "item", "description": "10.1002/cbic.202000051", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/cbic.202000051"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-03T00:00:00Z"}}, {"id": "10.1002/ece3.8676", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:14:04Z", "type": "Journal Article", "created": "2022-03-08", "title": "Effects of operational taxonomic unit inference methods on soil microeukaryote community analysis using long-read metabarcoding", "description": "Abstract

Long amplicon metabarcoding has opened the door for phylogenetic analysis of the largely unknown communities of microeukaryotes in soil. Here, we amplified and sequenced the ITS and LSU regions of the rDNA operon (around 1500\uffc2\uffa0bp) from grassland soils using PacBio SMRT sequencing. We tested how three different methods for generation of operational taxonomic units (OTUs) effected estimated richness and identified taxa, and how well large\uffe2\uff80\uff90scale ecological patterns associated with shifting environmental conditions were recovered in data from the three methods. The field site at Kungs\uffc3\uffa4ngen Nature Reserve has drawn frequent visitors since Linnaeus's time, and its species rich vegetation includes the largest population of Fritillaria meleagris in Sweden. To test the effect of different OTU generation methods, we sampled soils across an abrupt moisture transition that divides the meadow community into a Carex acuta dominated plant community with low species richness in the wetter part, which is visually distinct from the mesic\uffe2\uff80\uff90dry part that has a species rich grass\uffe2\uff80\uff90dominated plant community including a high frequency of F.\uffc2\uffa0meleagris. We used the moisture and plant community transition as a framework to investigate how detected belowground microeukaryotic community composition was influenced by OTU generation methods. Soil communities in both moisture regimes were dominated by protists, a large fraction of which were taxonomically assigned to Ciliophora (Alveolata) while 30%\uffe2\uff80\uff9340% of all reads were assigned to kingdom Fungi. Ecological patterns were consistently recovered irrespective of OTU generation method used. However, different methods strongly affect richness estimates and the taxonomic and phylogenetic resolution of the characterized community with implications for how well members of the microeukaryotic communities can be recognized in the data.

", "keywords": ["580", "species hypothesis", "Ekologi", "0301 basic medicine", "0303 health sciences", "Ecology", "rDNA", "Biological Systematics", "15. Life on land", "03 medical and health sciences", "14. Life underwater", "ITS", "Research Articles", "clustering"]}, "links": [{"href": "https://pub.epsilon.slu.se/27699/1/eshghi-sahraei-s-et-al-220505.pdf"}, {"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.8676"}, {"href": "https://doi.org/10.1002/ece3.8676"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.8676", "name": "item", "description": "10.1002/ece3.8676", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.8676"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-03-01T00:00:00Z"}}, {"id": "10.1002/ece3.1867", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:14:03Z", "type": "Journal Article", "created": "2016-01-11", "title": "Grazing Exclusion Reduced Soil Respiration But Increased Its Temperature Sensitivity In A Meadow Grassland On The Tibetan Plateau", "description": "Abstract

Understanding anthropogenic influences on soil respiration (Rs) is critical for accurate predictions of soil carbon fluxes, but it is not known how Rs responds to grazing exclusion (GE). Here, we conducted a manipulative experiment in a meadow grassland on the Tibetan Plateau to investigate the effects of GE on Rs. The exclusion of livestock significantly increased soil moisture and above\uffe2\uff80\uff90ground biomass, but it decreased soil temperature, microbial biomass carbon (MBC), and Rs. Regression analysis indicated that the effects of GE on Rs were mainly due to changes in soil temperature, soil moisture, and MBC. Compared with the grazed blocks, GE significantly decreased soil carbon release by 23.6% over the growing season and 21.4% annually, but it increased the temperature sensitivity (Q10) of Rs by 6.5% and 14.2% for the growing season and annually respectively. Therefore, GE may reduce the release of soil carbon from the Tibetan Plateau, but under future climate warming scenarios, the increases in Q10 induced by GE could lead to increased carbon emissions.

", "keywords": ["570", "MICROBIAL RESPIRATION", "Environmental Sciences & Ecology", "Plant Productivity", "Temperature Sensitivity", "ALPINE GRASSLAND", "630", "Microbial Biomass Carbon", "NORTHERN CHINA", "SEASONAL PATTERNS", "MOUNTAIN GRASSLANDS", "Grazing Exclusion", "Tibetan Plateau", "PLANT-COMMUNITIES", "Original Research", "2. Zero hunger", "Science & Technology", "CLIMATE-CHANGE", "CO2 EFFLUX", "Ecology", "04 agricultural and veterinary sciences", "15. Life on land", "INNER-MONGOLIA", "BELOW-GROUND BIOMASS", "Soil Respiration", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Life Sciences & Biomedicine"]}, "links": [{"href": "https://doi.org/10.1002/ece3.1867"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.1867", "name": "item", "description": "10.1002/ece3.1867", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.1867"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-01-11T00:00:00Z"}}, {"id": "10.1002/ece3.1879", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-09-20T16:14:03Z", "type": "Journal Article", "created": "2016-01-22", "title": "Effects Of Fertilization On Microbial Abundance And Emissions Of Greenhouse Gases (Ch4 And N2o) In Rice Paddy Fields", "description": "Abstract

This study is to explore effects of nitrogen application and straw incorporation on abundance of relevant microbes and CH4 and N2O fluxes in a midseason aerated rice paddy field. Fluxes of CH4 and N2O were recorded, and abundance of relevant soil microbial functional genes was determined during rice\uffe2\uff80\uff90growing season in a 6\uffe2\uff80\uff90year\uffe2\uff80\uff90long fertilization experiment field in China. Results indicate that application of urea significantly changed the functional microbial composition, while the influence of straw incorporation was not significant. Application of urea significantly decreased the gene abundances of archaeal amoA and mcrA, but it significantly increased the gene abundances of bacterial amoA. CH4 emission was significantly increased by fresh straw incorporation. Incorporation of burnt straw tended to increase CH4 emission, while the urea application had no obvious effect on CH4 emission. N2O emission was significantly increased by urea application, while fresh or burnt straw incorporation tended to decrease N2O emission. The functional microbial composition did not change significantly over time, although the abundances of pmoA, archaeal amoA, nirS, and nosZ genes changed significantly. The change of CH4 emission showed an inverse trend with the one of the N2O emissions over time. To some extent, the abundance of some functional genes in this study can explain CH4 and N2O emissions. However, the correlation between CH4 and N2O emissions and the abundance of related functional genes was not significant. Environmental factors, such as soil Eh, may be more related to CH4 and N2O emissions.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "03 medical and health sciences", "13. Climate action", "15. Life on land", "Original Research"]}, "links": [{"href": "https://doi.org/10.1002/ece3.1879"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.1879", "name": "item", "description": "10.1002/ece3.1879", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.1879"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-01-22T00:00:00Z"}}, {"id": "10.1002/ece3.6547", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:14:04Z", "type": "Journal Article", "created": "2020-07-09", "title": "The distribution of herbivores between leaves matches their performance only in the absence of competitors", "description": "Abstract

Few studies have tested how plant quality and the presence of competitors interact in determining how herbivores choose between different leaves within a plant. We investigated this in two herbivorous spider mites sharing tomato plants: Tetranychus urticae, which generally induces plant defenses, and Tetranychus evansi, which suppresses them, creating asymmetrical effects on coinfesting competitors. On uninfested plants, both herbivore species preferred young leaves, coinciding with increased mite performance. On plants with heterospecifics, the mites did not prefer leaves on which they had a better performance. In particular, T.\uffc2\uffa0urticae avoided leaves infested with T.\uffc2\uffa0evansi, which is in agreement with T.\uffc2\uffa0urticae being outcompeted by T.\uffc2\uffa0evansi. In contrast, T.\uffc2\uffa0evansi did not avoid leaves with the other species, but distributed itself evenly over plants infested with heterospecifics. We hypothesize that this behavior of T.\uffc2\uffa0evansi may prevent further spread of T.\uffc2\uffa0urticae over the shared plant. Our results indicate that leaf age determines within\uffe2\uff80\uff90plant distribution of herbivores only in absence of competitors. Moreover, they show that this distribution depends on the order of arrival of competitors and on their effects on each other, with herbivores showing differences in behavior within the plant as a possible response to the outcome of those interactions.The study of biodiversity has grown exponentially in the last thirty years in response to demands for greater understanding of the function and importance of Earth's biodiversity and finding solutions to conserve it. Here, we test the hypothesis that biodiversity science has become more interdisciplinary over time. To do so, we analyze 97,945 peer\uffe2\uff80\uff90reviewed articles over a twenty\uffe2\uff80\uff90two\uffe2\uff80\uff90year time period (1990\uffe2\uff80\uff932012) with a continuous time dynamic model, which classifies articles into concepts (i.e., topics and ideas) based on word co\uffe2\uff80\uff90occurrences. Using the model output, we then quantify different aspects of interdisciplinarity: concept diversity, that is, the diversity of topics and ideas across subdisciplines in biodiversity science, subdiscipline diversity, that is, the diversity of subdisciplines across concepts, and network structure, which captures interactions between concepts and subdisciplines. We found that, on average, concept and subdiscipline diversity in biodiversity science were either stable or declining, patterns which were driven by the persistence of rare concepts and subdisciplines and a decline in the diversity of common concepts and subdisciplines, respectively. Moreover, our results provide evidence that conceptual homogenization, that is, decreases in temporal \uffce\uffb2 concept diversity, underlies the observed trends in interdisciplinarity. Together, our results reveal that biodiversity science is undergoing a dynamic phase as a scientific discipline that is consolidating around a core set of concepts. Our results suggest that progress toward addressing the biodiversity crisis via greater interdisciplinarity during the study period may have been slowed by extrinsic factors, such as the failure to invest in research spanning across concepts and disciplines. However, recent initiatives such as the Intergovernmental Science\uffe2\uff80\uff90Policy Platform on Biodiversity and Ecosystem Services (IPBES) may attract broader support for biodiversity\uffe2\uff80\uff90related issues and hence interdisciplinary approaches to address scientific, political, and societal challenges in the coming years.

", "keywords": ["0301 basic medicine", "bibliographic analysis", "topic models", "0303 health sciences", "03 medical and health sciences", "conceptual homogenization", "interdisciplinarity", "Ecology", "13. Climate action", "15. Life on land", "Academic Practice in Ecology and Evolution", "QH540-549.5", "biodiversity"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.5244"}, {"href": "https://doi.org/10.1002/ece3.5244"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.5244", "name": "item", "description": "10.1002/ece3.5244", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.5244"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-01T00:00:00Z"}}, {"id": "10.1002/ecs2.2226", "type": "Feature", "geometry": null, "properties": {"updated": "2026-09-20T16:14:04Z", "type": "Journal Article", "created": "2018-05-31", "title": "Mycorrhiza in tree diversity-ecosystem function relationships: conceptual framework and experimental implementation", "description": "Abstract

The widely observed positive relationship between plant diversity and ecosystem functioning is thought to be substantially driven by complementary resource use of plant species. Recent work suggests that biotic interactions among plants and between plants and soil organisms drive key aspects of resource use complementarity. Here, we provide a conceptual framework for integrating positive biotic interactions across guilds of organisms, more specifically between plants and mycorrhizal types, to explain resource use complementarity in plants and its consequences for plant competition. Our overarching hypothesis is that ecosystem functioning increases when more plant species associate with functionally dissimilar mycorrhizal fungi because differing mycorrhizal types will increase coverage of habitat space for and reduce competition among plants. We introduce a recently established field experiment (MyDiv) that uses different pools of tree species that associate with either arbuscular or ectomycorrhizal fungi to create orthogonal experimental gradients in tree species richness and mycorrhizal associations and present initial results. Finally, we discuss options for future mechanistic studies on resource use complementarity within MyDiv. We show how mycorrhizal types and biotic interactions in MyDiv can be used in the future to test novel questions regarding the mechanisms underlying biodiversity\uffe2\uff80\uff93ecosystem function relationships.

", "keywords": ["0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "biodiversity\u2013ecosystem functioning", "experimental design", "Ecology", "arbuscular mycorrhiza", "15. Life on land", "01 natural sciences", "ectomycorrhiza", "Article", "biotic interactions", "03 medical and health sciences", "biodiversity effects", "QH540-549.5"]}, "links": [{"href": "https://esajournals.onlinelibrary.wiley.com/doi/pdf/10.1002/ecs2.2226"}, {"href": "https://doi.org/10.1002/ecs2.2226"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ecs2.2226", "name": "item", "description": "10.1002/ecs2.2226", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ecs2.2226"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-05-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Medicine&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Medicine&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Medicine&", "hreflang": "en-US"}, {"rel": "next", "type": "application/geo+json", "title": "items (next)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Medicine&offset=50", "hreflang": "en-US"}], "numberMatched": 2398, "numberReturned": 50, "distributedFeatures": [], "timeStamp": "2026-09-20T20:06:32.227043Z"}