{"type": "FeatureCollection", "features": [{"id": "10.1016/j.radmeas.2019.106221", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:16:38Z", "type": "Journal Article", "created": "2019-11-19", "title": "Bleaching studies on Al-hole ([AlO4/h]0) electron spin resonance (ESR) signal in sedimentary quartz", "description": "Abstract Electron spin resonance (ESR) dating of sediments using quartz is most commonly used for older sediments (>100 ka), since large residuals render the ESR signal unsuitable for dating young sediments. The multiple-centre approach (utilising both Ti and [AlO4/h]0 signals) is usually used to test the resetting of the signals used for ESR dating. Here we work towards a better understanding of, and correction for, the residual signal in ESR samples of sedimentary quartz. We undertook multiple-centre ESR measurements using quartz [AlO4/h]0 and Ti signals on young aeolian samples of different grain sizes which have been independently dated using optically stimulated luminescence (OSL). Our results demonstrate that [AlO4/h]0 signal yields residuals indicating equivalent doses of about 500\u00a0Gy, substantially older than expected for the known OSL equivalent doses in the range of 8\u201337\u00a0Gy. The decay of [AlO4/h]0 signal as function of bleaching time can be represented by an exponential function. We investigate the dependence of the residual magnitude of the ESR signal as a function of the previous given dose and observe an exponential increase in the residual signal with dose. Such observations are consistent with the results of luminescence process modelling conducted for a model comprising two luminescence centres and several traps, one of which is a so-called deep disconnected trap that cannot be emptied during optical stimulation. We propose that bleaching occurs through an electron-hole recombination process with electrons released from optically sensitive traps. In addition to our new insights into the bleaching mechanisms of the [AlO4/h]0 ESR signal, we discuss the implications for the procedures used for performing residual dose corrections in ESR dating. We recommend that modern analogues be used in addition to laboratory-bleached samples when performing residual dose corrections.", "keywords": ["ESR dating", "Sedimentary quartz", "03 medical and health sciences", "0302 clinical medicine", "Residual", "Sedimentary quartz ESR dating[AlO4/h]0 Residual Bleaching Modelling", "[AlO4/h]0", "Bleaching", "01 natural sciences", "Modelling", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.radmeas.2019.106221"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Radiation%20Measurements", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.radmeas.2019.106221", "name": "item", "description": "10.1016/j.radmeas.2019.106221", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.radmeas.2019.106221"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-01T00:00:00Z"}}, {"id": "10.1038/s41598-022-23318-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:17:36Z", "type": "Journal Article", "created": "2022-11-05", "title": "Optical photothermal infrared spectroscopy with simultaneously acquired Raman spectroscopy for two-dimensional microplastic identification", "description": "Abstract

In recent years, vibrational spectroscopic techniques based on Fourier transform infrared (FTIR) or Raman microspectroscopy have been suggested to fulfill the unmet need for microplastic particle detection and identification. Inter-system comparison of spectra from reference polymers enables assessing the reproducibility between instruments and advantages of emerging quantum cascade laser-based optical photothermal infrared (O-PTIR) spectroscopy. In our work, IR and Raman spectra of nine plastics, namely polyethylene, polypropylene, polyvinyl chloride, polyethylene terephthalate, polycarbonate, polystyrene, silicone, polylactide acid and polymethylmethacrylate were simultaneously acquired using an O-PTIR microscope in non-contact, reflection mode. Comprehensive band assignments were presented. We determined the agreement of O-PTIR with standalone attenuated total reflection FTIR and Raman spectrometers based on the hit quality index (HQI) and introduced a two-dimensional identification (2D-HQI) approach using both Raman- and IR-HQIs. Finally, microplastic particles were prepared as test samples from known materials by wet grinding, O-PTIR data were collected and subjected to the 2D-HQI identification approach. We concluded that this framework offers improved material identification of microplastic particles in environmental, nutritious and biological matrices.

<p style='color: #000000; font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 10px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;'>Every year, 40 million women give birth at home without the help of a<br />skilled birth attendant. In 2011, 287,000 women died during pregnancy or childbirth. Almost all these deaths occur in developing countries where mothers and children lack access to basic health care. Reports showed the low utilization of health facility for delivery service in Ethiopia. This study aimed to determine the utilization and factors influencing institutional delivery. Community based cross sectional study was conducted from January to February 2013/14 in Boricha District of Southern Ethiopia among mothers who gave birth in the last 1 year. Multistage sampling techniques were used to collect data from 546 mothers. Taking in to account place of birth for the last child, only 4.9% women gave birth in a health facility. Women\u2019s education level (AOR=4.4 (95% CI=1.36-14.33)), timing of firstANC visit (AOR= .03 (95% CI=0.004 - 0.205)), women\u2019s advice to deliver in a health facility during ANC (AOR = 31.15 (95% CI=2.02-479.52)), women\u2019s knowledge of birth related complications (AOR= 12.4 (95% CI=2.67-57.16)) and decision making power (AOR=0.2 (95% CI=0.060.82)) showed significant association with institutionional delivery. Institutional delivery in the study area was found to be very low. Raising\u00a0awareness on institutional delivery to maximize delivery service utilization and strengthening provision of education and counseling to deliver in health facility during antenatal care visits at individual and community level should be given due emphasis.</p>

", "keywords": ["2. Zero hunger", "03 medical and health sciences", "0302 clinical medicine", "1. No poverty", "3. Good health"]}, "links": [{"href": "https://doi.org/10.11591/.v3i4.4696"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Public%20Health%20Science%20%28IJPHS%29", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.11591/.v3i4.4696", "name": "item", "description": "10.11591/.v3i4.4696", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.11591/.v3i4.4696"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2014-12-01T00:00:00Z"}}, {"id": "10.3389/fmicb.2016.01446", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:20:15Z", "type": "Journal Article", "created": "2016-09-14", "description": "Soil management is fundamental to all agricultural systems and fertilization practices have contributed substantially to the impressive increases in food production. Despite the pivotal role of soil microorganisms in agro-ecosystems, we still have a limited understanding of the complex response of the soil microbiota to organic and mineral fertilization in the very long-term. Here, we report the effects of different fertilization regimes (mineral, organic and combined mineral and organic fertilization), carried out for more than a century, on the structure and activity of the soil microbiome. Organic matter content, nutrient concentrations, and microbial biomass carbon were significantly increased by mineral, and even more strongly by organic fertilization. Pyrosequencing revealed significant differences between the structures of bacterial and fungal soil communities associated to each fertilization regime. Organic fertilization increased bacterial diversity, and stimulated microbial groups (Firmicutes, Proteobacteria, and Zygomycota) that are known to prefer nutrient-rich environments, and that are involved in the degradation of complex organic compounds. In contrast, soils not receiving manure harbored distinct microbial communities enriched in oligotrophic organisms adapted to nutrient-limited environments, as Acidobacteria. The fertilization regime also affected the relative abundances of plant beneficial and detrimental microbial taxa, which may influence productivity and stability of the agroecosystem. As expected, the activity of microbial exoenzymes involved in carbon, nitrogen, and phosphorous mineralization were enhanced by both types of fertilization. However, in contrast to comparable studies, the highest chitinase and phosphatase activities were observed in the solely mineral fertilized soil. Interestingly, these two enzymes showed also a particular high biomass-specific activities and a strong negative relation with soil pH. As many soil parameters are known to change slowly, the particularity of unchanged fertilization treatments since 1902 allows a profound assessment of linkages between management and abiotic as well as biotic soil parameters. Our study revealed that pH and TOC were the majors, while nitrogen and phosphorous pools were minors, drivers for structure and activity of the soil microbial community. Due to the long-term treatments studied, our findings likely represent permanent and stable, rather than transient, responses of soil microbial communities to fertilization.", "keywords": ["Soil nutrients", "0301 basic medicine", "2. Zero hunger", "0303 health sciences", "long-term fertilization", "microbial biomass", "15. Life on land", "microbial activity", "Microbiology", "QR1-502", "03 medical and health sciences", "13. Climate action", "soil microbial communities", "soil nutrients", "454 pyrosequencing"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2016.01446"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2016.01446", "name": "item", "description": "10.3389/fmicb.2016.01446", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2016.01446"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-14T00:00:00Z"}}, {"id": "10.3389/fmicb.2019.02597", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:20:15Z", "type": "Journal Article", "created": "2019-11-08", "title": "New Insights Into Cinnamoyl Esterase Activity of Oenococcus oeni.", "description": "Some strains of Oenococcus oeni possess cinnamoyl esterase activity that can be relevant in the malolactic stage of wine production liberating hydroxycinnamic acids that are precursors of volatile phenols responsible for sensory faults. The objective of this study was to better understand the basis of the differential activity between strains. After initial screening, five commercial strains of O. oeni were selected, three were found to exhibit cinnamoyl esterase activity (CE+) and two not (CE-). Although the use of functional annotation of genes revealed genotypic variations between the strains, no specific genes common only to the three CE+ strains could explain the different activities. Pasteurized wine was used as a natural source of tartrate esters in growth and metabolism experiments conducted in MRS medium, whilst commercial trans-caftaric acid was used as substrate for enzyme assays. Detoxification did not seem to be the main biological mechanism involved in the activity since unlike its phenolic cleavage products and their immediate metabolites (trans-caffeic acid and 4-ethylcatechol), trans-caftaric acid was not toxic toward O. oeni. In the case of the two CE+ strains OenosTM and CiNeTM, wine-exposed samples showed a more rapid degradation of trans-caftaric acid than the unexposed ones. The CE activity was present in all cell-free extracts of both wine-exposed and unexposed strains, except in the cell-free extracts of the CE- strain CH11TM. This activity may be constitutive rather than induced by exposure to tartrate esters. Trans-caftaric acid was totally cleaved to trans-caffeic acid by cell-free extracts of the three CE+ strains, whilst cell-free extracts of the CE- strain CH16TM showed significantly lower activity, although higher for the strains in experiments with no prior wine exposure. The EstB28 esterase gene, found in the genomes of the 5 strains, did not reveal any difference on the upstream regulation and transport functionality between the strains. This study highlights the complexity of the basis of this activity in wine related O. oeni population. Variable cinnamoyl esterases or/and membrane transport activities in the O. oeni strains analyzed and a possible implication of wine molecules could explain this phenomenon.", "keywords": ["0301 basic medicine", "0303 health sciences", "tartrate esters", "cinnamoyl esterase", "Tartrate esters", "Hydroxycinnamic acids", "Wine", "hydroxycinnamic acids", "[SDV.IDA] Life Sciences [q-bio]/Food engineering", "Microbiology", "QR1-502", "03 medical and health sciences", "Cinnamoyl esterase", "wine", "Oenococcus oeni"]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2019.02597"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2019.02597", "name": "item", "description": "10.3389/fmicb.2019.02597", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2019.02597"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-11-08T00:00:00Z"}}, {"id": "10.3389/fpls.2017.01892", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:20:17Z", "type": "Journal Article", "created": "2017-11-06", "title": "Variability in Susceptibility to Anthracnose in the World Collection of Olive Cultivars of Cordoba (Spain)", "description": "Anthracnose of olive (Olea europaea ssp. europaea L.), caused by Colletotrichum species, is a serious disease causing fruit rot and branch dieback, whose epidemics are highly dependent on cultivar susceptibility and environmental conditions. Over a period of 10 years, there have been three severe epidemics in Andalusia (southern Spain) that allowed us to complete the assessment of the World Olive Germplasm Bank of C\u00f3rdoba, one of the most important cultivar collections worldwide.A total of 308 cultivars from 21 countries were evaluated, mainly Spain (174 cvs.), Syria (29 cvs.), Italy (20 cvs.), Turkey (15 cvs.), and Greece (16 cvs.). Disease assessments were performed using a 0-10 rating scale, specifically developed to estimate the incidence of symptomatic fruit in the tree canopy. Also, the susceptibility of five reference cultivars was confirmed by artificial inoculation. Because of the direct relationship between the maturity of the fruit and their susceptibility to the pathogen, evaluations were performed at the end of fruit ripening, which forced coupling assessments according to the maturity state of the trees. By applying the cluster analysis to the 308 cultivars, these were classified as follows: 66 cvs. highly susceptible (21.4%), 83 cvs. susceptible (26.9%), 66 cvs. moderately susceptible (21.4%), 61 cvs. resistant (19.8%), and 32 cvs. highly resistant (10.4%). Representative cultivars of these five categories are 'Ocal,' 'Lech\u00edn de Sevilla,' 'Arbequina,' 'Picual,' and 'Frantoio,' respectively. With some exceptions, such as cvs. Arbosana, Empeltre and Picual, most of the Spanish cultivars, such as 'Arbequina,' 'Cornicabra,' 'Hojiblanca,' 'Manzanilla de Sevilla,' 'Morisca,' 'Picudo,' 'Farga,' and 'Verdial de Hu\u00e9var' are included in the categories of moderately susceptible, susceptible or highly susceptible. The phenotypic evaluation of anthracnose reaction is a limiting factor for the selection of olive cultivars by farmers, technicians, and breeders.", "keywords": ["0301 basic medicine", "2. Zero hunger", "anthracnose", "0303 health sciences", "Plant culture", "Plant Science", "15. Life on land", "16. Peace & justice", "olive", "diseases", "SB1-1110", "03 medical and health sciences", "fruit rot", "Colletotrichum"], "contacts": [{"organization": "J. R. Viruega, Luis F. Roca, Carlos Xavi\u00e9r, Juan Caballero, Antonio Trapero, Juan Moral, Juan Moral,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.3389/fpls.2017.01892"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Plant%20Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fpls.2017.01892", "name": "item", "description": "10.3389/fpls.2017.01892", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fpls.2017.01892"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-11-06T00:00:00Z"}}, {"id": "10.3389/fmicb.2023.1197770", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:20:16Z", "type": "Journal Article", "created": "2023-05-24", "title": "Effects of tomato inoculation with the entomopathogenic fungus Metarhizium brunneum on spider mite resistance and the rhizosphere microbial community", "description": "

Entomopathogenic fungi have been well exploited as biocontrol agents that can kill insects through direct contact. However, recent research has shown that they can also play an important role as plant endophytes, stimulating plant growth, and indirectly suppressing pest populations. In this study, we examined the indirect, plant-mediated, effects of a strain of entomopathogenic fungus, Metarhizium brunneum on plant growth and population growth of two-spotted spider mites (Tetranychus urticae) in tomato, using different inoculation methods (seed treatment, soil drenching and a combination of both). Furthermore, we investigated changes in tomato leaf metabolites (sugars and phenolics), and rhizosphere microbial communities in response to M. brunneum inoculation and spider mite feeding. A significant reduction in spider mite population growth was observed in response to M. brunneum inoculation. The reduction was strongest when the inoculum was supplied both as seed treatment and soil drench. This combination treatment also yielded the highest shoot and root biomass in both spider mite-infested and non-infested plants, while spider mite infestation increased shoot but reduced root biomass. Fungal treatments did not consistently affect leaf chlorogenic acid and rutin concentrations, but M. brunneum inoculation via a combination of seed treatment and soil drenching reinforced chlorogenic acid (CGA) induction in response to spider mites and under these conditions the strongest spider mite resistance was observed. However, it is unclear whether the M. brunneum-induced increase in CGA contributed to the observed spider mite resistance, as no general association between CGA levels and spider mite resistance was observed. Spider mite infestation resulted in up to two-fold increase in leaf sucrose concentrations and a three to five-fold increase in glucose and fructose concentrations, but these concentrations were not affected by fungal inoculation. Metarhizium, especially when applied as soil drench, impacted the fungal community composition but not the bacterial community composition which was only affected by the presence of spider mites. Our results suggest that in addition to directly killing spider mites, M. brunneum can indirectly suppress spider mite populations on tomato, although the underlying mechanism has not yet been resolved, and can also affect the composition of the soil microbial community.Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses to salinity, we developed and exploited a real-time salt-induced tilting assay. This assay follows root growth upon both gravitropic and salt challenges, revealing that root bending upon tilting is modulated by Na+ ions, but not by osmotic stress. Next, we measured this salt-specific response in 345 natural Arabidopsis (Arabidopsis thaliana) accessions and discovered a genetic locus, encoding the cell wall-modifying enzyme EXTENSIN ARABINOSE DEFICIENT TRANSFERASE (ExAD) that is associated with root bending in the presence of NaCl (hereafter salt). Extensins are a class of structural cell wall glycoproteins known as hydroxyproline (Hyp)-rich glycoproteins, which are posttranslationally modified by O-glycosylation, mostly involving Hyp-arabinosylation. We show that salt-induced ExAD-dependent Hyp-arabinosylation influences root bending responses and cell wall thickness. Roots of exad1 mutant seedlings, which lack Hyp-arabinosylation of extensin, displayed increased thickness of root epidermal cell walls and greater cell wall porosity. They also showed altered gravitropic root bending in salt conditions and a reduced salt-avoidance response. Our results suggest that extensin modification via Hyp-arabinosylation is a unique salt-specific cellular process required for the directional response of roots exposed to salinity.Drylands (hyperarid, arid, semiarid, and dry subhumid ecosystems) cover almost half of Earth\uffe2\uff80\uff99s land surface and are highly vulnerable to environmental pressures. Here we provide an inventory of soil properties including carbon (C), nitrogen (N), and phosphorus (P) stocks within the current boundaries of drylands, aimed at serving as a benchmark in the face of future challenges including increased population, food security, desertification, and climate change. Aridity limits plant production and results in poorly developed soils, with coarse texture, low C:N and C:P, scarce organic matter, and high vulnerability to erosion. Dryland soils store 646 Pg of organic C to 2\uffe2\uff80\uff89m, the equivalent of 32% of the global soil organic C pool. The magnitude of the historic loss of C from dryland soils due to human land use and cover change and their typically low C:N and C:P suggest high potential to build up soil organic matter, but coarse soil textures may limit protection and stabilization processes. Restoring, preserving, and increasing soil organic matter in drylands may help slow down rising levels of atmospheric carbon dioxide by sequestering C, and is strongly needed to enhance food security and reduce the risk of land degradation and desertification.Soil moisture is among the most important factors regulating soil biodiversity and functioning. Models forecast changes in the precipitation regime in many areas of the planet, but how these changes will influence soil functioning, and how biotic drivers modulate such effects, is far from being understood. We evaluated the responses of C and N fluxes, and soil microbial properties to different soil water regimes in soils from the main three ecotypes of the world's largest and most diverse tropical savanna. Further, we explored the direct and indirect effects of changes in the ecotype and soil water regimes on these key soil processes. Soils from the woodland savanna showed a better nutritional status than the other ecotypes, as well as higher potential N cycling rates, N2O emissions, and soil bacterial abundance but lower bacterial richness, whereas potential CO2 emissions and CH4 uptake peaked in the intermediate savanna. The ecotype also modulated the effects of changes in the soil water regime on nutrient cycling, greenhouse gas fluxes, and soil bacterial properties, with more intense responses in the intermediate savanna. Further, we highlight the existence of multiple contrasting direct and indirect (via soil microbes and abiotic properties) effects of an intensification of the precipitation regime on soil C- and N-related processes. Our results confirm that ecotype is a fundamental driver of soil properties and functioning in the Cerrado and that it can determine the responses of key soil processes to changes in the soil water regime.Theory suggests that the diversity of molecules in soil organic matter (SOM functional diversity) provides key insights on multiple ecosystem services. We aimed to investigate how and why SOM functional diversity and composition change as topsoils develop, and its implications for key soil functions (e.g., from nutrient pool to water regulation).

Location

We reported data on 16 soil chronosequences globally distributed in nine countries from six continents.

Time Period

2016\uffe2\uff80\uff932017.

Major Taxa Studied

Soil microbes (bacteria and fungi) and vascular plants.

Methods

SOM functional diversity and composition without mineral interference were measured using diffuse reflectance mid\uffe2\uff80\uff90infrared Fourier transform spectroscopy (DRIFT). We aimed to characterize the main environmental factors related to SOM functional diversity and composition. Also, we calculated the links among SOM functional diversity and key soil functions.

Results

We found that SOM functional diversity declines after millions of years of soil formation (pedogenesis). We further showed that increases in plant cover and productivity led to a higher ratio of reduced (e.g., alkanes) over oxidized carbon forms (i.e., C: O\uffe2\uff80\uff90functional groups ratio), which was positively correlated to SOM functional diversity as soils age. Our findings indicated that the plant footprint (i.e., the accumulation of plant\uffe2\uff80\uff90derived material promoting the C: O\uffe2\uff80\uff90functional group ratio) would explain the reduction of SOM functional diversity as ecosystems develop. Moreover, the dissimilarity in SOM composition consistently increased with soil age, with the soil development stage emerging as the main predictor of SOM dissimilarity across contrasting biomes.

Main Conclusions

Our global survey contextualized the natural history of SOM functional diversity and composition during long\uffe2\uff80\uff90term soil development. Together, we showed how plant footprint drives the losses of SOM functional diversity with increasing age, which might provide a novel mechanism to explain typically reported losses in ecosystem functions during ecosystem retrogression.
We incubated temperate forest soil at constant temperature (20 \u00b0C) and water content, and exposed it to strong single-pulse perturbations, which nonetheless mimic common pulse-events in temperate soils (glucose addition at 4 mg g\u22121 soil, or freeze-thawing overnight at \u221220 \u00b0C). We subsequently measured microbial community composition and microbial storage compounds via phospho- and neutral lipid fatty acid (PLFA and NLFA) profiling, as well as C/N stoichiometry of microbial biomass and dissolved organic carbon and nitrogen in the soil solution shortly after (0.4, 1, 4, and 6 days) and after longer time periods (84 and 160 days) following the perturbations.

Transferring the soils from their natural environment to the laboratory and incubating them under controlled conditions led to a continuous change of microbial community structure over time, along with an increase in microbial biomass and dissolved N in both perturbed and control soils over the time of the experiment. Against the background of this \u2018press-disturbance\u2019, caused by the permanently changed conditions, we see immediate and long-lasting effects of the single pulse events on microbial community composition, C storage and C/N stoichiometry. Both perturbations significantly influenced the microbial community structure (based on PLFA profiles), microbial biomass N and dissolved N up to 160 days, as well as fungal and bacterial biomass and storage (based on absolute PLFA and NLFA concentrations) up to 84 days. Both perturbations increased microbial N (+59.6 \u00b5g g\u22121 dw) and decreased dissolved N (\u221240.3 \u00b5g g\u22121 dw) after 160 days, and significantly altered C/N ratios in microbial and dissolved pools (particularly in the first 6 days of the experiment).

Our results demonstrate that single-pulse perturbations can have long-term legacies in soil microbial ecosystems. In our experiment they led to alternative system states which differed from the unperturbed control in multiple parameters even after 160 days. This indicates that soil microbial communities exhibit a low resistance and resilience towards single-pulse perturbations, and may easily be pushed on alternative trajectories by short but strong environmental pulses.", "keywords": ["0301 basic medicine", "2. Zero hunger", "106022 Mikrobiologie", "0303 health sciences", "Resilience", "04 agricultural and veterinary sciences", "15. Life on land", "Perturbations", "6. Clean water", "Transient state", "Pulse event", "03 medical and health sciences", "106026 \u00d6kosystemforschung", "13. Climate action", "Soil microbial community", "106022 Microbiology", "0401 agriculture", " forestry", " and fisheries", "106026 Ecosystem research"]}, "links": [{"href": "https://doi.org/10.1016/j.geoderma.2023.116399"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Geoderma", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.geoderma.2023.116399", "name": "item", "description": "10.1016/j.geoderma.2023.116399", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.geoderma.2023.116399"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-04-01T00:00:00Z"}}, {"id": "10.1016/j.envint.2020.106190", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:16:04Z", "type": "Journal Article", "created": "2020-10-26", "title": "Treated wastewater irrigation promotes the spread of antibiotic resistance into subsoil pore-water", "description": "In the present study, we investigated the impact of treated wastewater (TWW) irrigation on the prevalence of antibiotic resistance genes (ARGs) in subsoil pore-water, a so-far under-appreciated matrix. We hypothesized that TWW irrigation increases ARG prevalence in subsoil pore-water. This hypothesis was tested using a multiphase approach, which consisted of sampling percolated subsoil pore-water from lysimeter-wells of a real-scale TWW-irrigated field, operated for commercial farming practices, and controlled, laboratory microcosms irrigated with freshwater or TWW. We monitored the abundance of six selected ARGs (sul1, blaOXA-58, tetM, qnrS, blaCTX-M-32 and blaTEM), the intI1 gene associated with mobile genetic elements and an indicator for anthropogenic pollution and bacterial abundance (16S rRNA gene) by qPCR. The bacterial load of subsoil pore water was independent of both, irrigation intensity in the field study and irrigation water type in the microcosms. Among the tested genes in the field study, sul1 and intI1 exhibited constantly higher relative abundances. Their abundance was further positively correlated with increasing irrigation intensity. Controlled microcosm experiments verified the observed field study results: the relative abundance of several genes, including sul1 and intI1, increased significantly when irrigating with TWW compared to freshwater irrigation. Overall, TWW irrigation promoted the spread of ARGs and intI1 in the subsoil pore-water, while the bacterial load was maintained. The combined results from the real-scale agricultural field and the controlled lab microcosms indicate that the dissemination of ARGs in various subsurface environments needs to be taken into account during TWW irrigation scenarios.", "keywords": ["0301 basic medicine", "2. Zero hunger", "Agricultural Irrigation", "Antibiotic resistance", "Water", "Subsoil pore-water", "Drug Resistance", " Microbial", "Wastewater", "Wastewater reuse", "01 natural sciences", "6. Clean water", "Anti-Bacterial Agents", "Environmental sciences", "qPCR", "Soil", "03 medical and health sciences", "Genes", " Bacterial", "RNA", " Ribosomal", " 16S", "ARGs", "GE1-350", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.1016/j.envint.2020.106190"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environment%20International", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envint.2020.106190", "name": "item", "description": "10.1016/j.envint.2020.106190", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envint.2020.106190"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "10.1016/j.biocontrol.2022.104890", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:15:46Z", "type": "Journal Article", "created": "2022-03-14", "title": "Exploring microbiomes for plant disease management", "description": "Microbiome science is revolutionizing many concepts of plant biology, ecology, and evolution. Understanding plant microbiomes is key to developing solutions that protect crop health without impacting the environment. In this perspective article, we highlight the importance of both the structure and functions of plant-associated microbial communities in protecting their host from pathogens. These new findings have a high potential to aid biocontrol programs and to replace traditional chemical products, guiding the transition towards a sustainable production.", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "ddc:570", "15. Life on land", "Institut f\u00fcr Biochemie und Biologie"]}, "links": [{"href": "https://doi.org/10.1016/j.biocontrol.2022.104890"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biological%20Control", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biocontrol.2022.104890", "name": "item", "description": "10.1016/j.biocontrol.2022.104890", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biocontrol.2022.104890"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-06-01T00:00:00Z"}}, {"id": "10451/51015", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:23:56Z", "type": "Journal Article", "created": "2021-11-19", "title": "Women\u2019s Empowerment, Research, and Management: Their Contribution to Social Sustainability", "description": "

This article enhances some ideas and opinions related with the challenges that women across the world face nowadays to pursue a professional career. The route they take has frequent obstacles of a distinct nature, which are not always comparable with those faced by men. The starting point of this work is the conclusions of the workshop \u201cWomen empowerment and gender equality, responsible research and innovation\u201d\u2014Research Project \u201cSOILdarity\u201d. This workshop revealed a diversified view about the subject, evidencing its connection with the social sustainability, with a special focus on academia and research management. This document introduces the topic in the context of the workshop, revises key aspects of women\u2019s empowerment in a society still dominated by men\u2019s opinions and organization (gender balance, obstacles and strategies), highlights the situation of women in academia and in research management, and gives the authors\u2019 opinions about some questions related to gender and equality. We conclude that all societal actors must acknowledge women as key actors in contributing to social sustainability, working together towards creating an environment where they can have leadership roles.

", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "non-paid work", "5. Gender equality", "gender and social perception", "women", "family and academia", "research management", "women; gender and social perception; social sustainability; research management; family and academia; non-paid work", "social sustainability", "12. Responsible consumption"]}, "links": [{"href": "https://repositorio.ulisboa.pt/bitstream/10451/60001/1/Ventura%20et%20al%202021.pdf"}, {"href": "https://repositorio.ulisboa.pt/bitstream/10451/51097/1/sustainability-13-12754.pdf"}, {"href": "https://repositorio.ulisboa.pt/bitstream/10451/51015/1/sustainability%20Women%20copy%202.pdf"}, {"href": "https://www.mdpi.com/2071-1050/13/22/12754/pdf"}, {"href": "https://doi.org/10451/51015"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Sustainability", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10451/51015", "name": "item", "description": "10451/51015", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10451/51015"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-11-18T00:00:00Z"}}, {"id": "10.3390/microorganisms8071093", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:20:29Z", "type": "Journal Article", "created": "2020-07-22", "title": "New insight into antimicrobial compounds from food and marine-sourced Carnobacterium species through phenotype and genome analyses.", "description": "

Carnobacterium maltaromaticum and Carnobacterium divergens, isolated from food products, are lactic acid bacteria known to produce active and efficient bacteriocins. Other species, particularly those originating from marine sources, are less studied. The aim of the study is to select promising strains with antimicrobial potential by combining genomic and phenotypic approaches on large datasets comprising 12 Carnobacterium species. The biosynthetic gene cluster (BGCs) diversity of 39 publicly available Carnobacterium spp. genomes revealed 67 BGCs, distributed according to the species and ecological niches. From zero to six BGCs were predicted per strain and classified into four classes: terpene, NRPS (non-ribosomal peptide synthetase), NRPS-PKS (hybrid non-ribosomal peptide synthetase-polyketide synthase), RiPP (ribosomally synthesized and post-translationally modified peptide). In parallel, the antimicrobial activity of 260 strains from seafood products was evaluated. Among the 60% of active strains, three genomes were sequenced and submitted to a dereplication process. C. inhibens MIP2551 produced a high amountof H2O2, probably thanks to the presence of four oxidase-encoding genes. C. maltaromaticum EBP3019 and SF668 strains were highly efficient against Listeria monocytogenes. A new extracellular 16 kDa unmodified bacteriocin in the EBP3019 strain and five different bacteriocins in SF668 were highlighted. In this study, the overview of antimicrobial BGC and inhibitory activities of Carnobacterium spp. allowed the prediction of potential innovative natural products that could be relevant for biotechnological applications.

", "keywords": ["Carnobacteriumspp", "0301 basic medicine", "2. Zero hunger", "570", "natural product", "antimicrobial activity", "QH301-705.5", "[SDV]Life Sciences [q-bio]", "NRPS", "hydrogen peroxide", "630", "Carnobacterium spp.", "Carnobacterium spp.", "Article", "lactic acid bacteria", "03 medical and health sciences", "bacteriocin", "genome mining", "RiPP", "Biology (General)", "terpene"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/8/7/1093/pdf"}, {"href": "https://www.mdpi.com/2076-2607/8/7/1093/pdf"}, {"href": "https://doi.org/10.3390/microorganisms8071093"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms8071093", "name": "item", "description": "10.3390/microorganisms8071093", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms8071093"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-07-21T00:00:00Z"}}, {"id": "10.1016/j.agee.2017.06.003", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:15:29Z", "type": "Journal Article", "created": "2017-06-10", "title": "Effects Of Shade-Tree Species And Spacing On Soil And Leaf Nutrient Concentrations In Cocoa Plantations At 8 Years After Establishment", "description": "Intercropping in agroforestry systems improves ecosystem services. Appropriate species compositions and spacing regimes are critical to achieve ecosystem benefits and improve yields of all the component crops. Cocoa (Theobroma cacao) is an important cash crop globally but it requires shade for survival and growth. However, the effects of shade-tree species composition and spacing regime on nutrient cycling in cocoa plantations are not well understood. This study investigated the effects of shade tree species and spacing regimes on soil and plant nutrient availability at 8 years after plantation establishment in Papua New Guinea. Three cocoa intercropping systems were established in which T. cacao was planted with either a non-legume timber tree, Canarium indicum, or a legume non-timber tree, Gliricidia sepium. The shade-tree spacing regimes included either 8 m \u00d7 16 m or 8 m \u00d7 8 m in the Theobroma + Canarium plantations. There was an ongoing thinning regime in the Theobroma + Gliricidia plantation, with a final shade-tree spacing of 12 m \u00d7 12 m. Soil total carbon (TC) and total nitrogen (TN) were significantly higher in the Theobroma + Gliricidia plantation with 12 m \u00d7 12 m spacing and the Theobroma + Canarium plantation with 8 m \u00d7 16 m spacing than in the Theobroma + Canarium plantation with 8 m \u00d7 8 m spacing. Foliar TN and P were correlated with soil TN and P, respectively, whereas no correlation was detected between soil and leaf K concentrations. Foliar TN, P and K were under ideal concentrations for T. cacao in all of the plantations. The Theobroma + Gliricidia plantation had higher soil water extractable phosphorus (P) than the two Theobroma + Canarium plantations, probably due to frequent pruning of the G. sepium trees. Foliar C isotope composition (\u03b413C) of T. cacao suggested that T. cacao close to G. sepium or close to C. indicum with spacing of 8 m \u00d7 16 m and 8 m \u00d7 8 m had similar light interception. However, increased C. indicum spacing increased the light interception of T. cacao trees that were not planted next to C. indicum. This study indicated that non-legume timber trees with an optimized spacing regime can be used as overstorey shade trees for T. cacao. However, our study indicated all three plantations required fertilisation and better nutrient management.", "keywords": ["571", "stable isotopes", "FoR 16 (Studies in Human Society)", "Canarium indicum", "Soil fertility", "Gliricidia sepium", "333", "630", "Papua New Guinea", "veterinary and food sciences", "Stable isotopes", "2. Zero hunger", "Field organic and low chemical input horticulture", "Agricultural", "Science & Technology", "Multidisciplinary", "Ecology", "soil fertility", "FoR 07 (Agricultural and Veterinary Sciences)", "Human society", "Agriculture", "04 agricultural and veterinary sciences", "15. Life on land", "Environmental sciences", "260516 Tropical fruit", "gliricidia sepium", "Intercropping", "070501 Agroforestry", "0401 agriculture", " forestry", " and fisheries", "FoR 05 (Environmental Sciences)", "intercropping", "Life Sciences & Biomedicine"]}, "links": [{"href": "https://doi.org/10.1016/j.agee.2017.06.003"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agriculture%2C%20Ecosystems%20%26amp%3B%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.agee.2017.06.003", "name": "item", "description": "10.1016/j.agee.2017.06.003", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.agee.2017.06.003"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-08-01T00:00:00Z"}}, {"id": "10.3390/genes15010107", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:20:26Z", "type": "Journal Article", "created": "2024-01-16", "title": "Structural and Functional Shifts in the Microbial Community of a Heavy Metal-Contaminated Soil Exposed to Short-Term Changes in Air Temperature, Soil Moisture and UV Radiation", "description": "

The interplay between metal contamination and climate change may exacerbate the negative impact on the soil microbiome and, consequently, on soil health and ecosystem services. We assessed the response of the microbial community of a heavy metal-contaminated soil when exposed to short-term (48 h) variations in air temperature, soil humidity or ultraviolet (UV) radiation in the absence and presence of Enchytraeus crypticus (soil invertebrate). Each of the climate scenarios simulated significantly altered at least one of the microbial parameters measured. Irrespective of the presence or absence of invertebrates, the effects were particularly marked upon exposure to increased air temperature and alterations in soil moisture levels (drought and flood scenarios). The observed effects can be partly explained by significant alterations in soil properties such as pH, dissolved organic carbon, and water-extractable heavy metals, which were observed for all scenarios in comparison to standard conditions. The occurrence of invertebrates mitigated some of the impacts observed on the soil microbial community, particularly in bacterial abundance, richness, diversity, and metabolic activity. Our findings emphasize the importance of considering the interplay between climate change, anthropogenic pressures, and soil biotic components to assess the impact of climate change on terrestrial ecosystems and to develop and implement effective management strategies.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "Soil invertebrates", "Soil microbiome", "Soil drought", "Ultraviolet Rays", "Soil pollution", "Microbiota", "Temperature", "Enchytraeus crypticus", "15. Life on land", "01 natural sciences", "Article", "6. Clean water", "Soil", "03 medical and health sciences", "13. Climate action", "UVR exposure", "Metals", " Heavy", "Climate change", "Soil flood", "Metagenomics", "Increased temperature", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://doi.org/10.3390/genes15010107"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Genes", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/genes15010107", "name": "item", "description": "10.3390/genes15010107", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/genes15010107"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-01-16T00:00:00Z"}}, {"id": "PMC11101930", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:27:06Z", "type": "Journal Article", "created": "2024-05-05", "title": "Robenidine derivatives as potential antischistosomal drug candidates", "description": "Schistosomiasis caused by Schistosoma spp. is a disease that causes a considerable health burden to millions of people worldwide. The limited availability of effective drugs on the market and the increased risk of resistance development due to extensive usage, highlight the urgent need for new antischistosomal drugs. Recent studies have shown that robenidine derivatives, containing an aminoguanidine core, exhibit promising activities against Plasmodium falciparum, motivating further investigation into their efficacy against Schistosoma mansoni, due to their similar habitat and the resulting related cellular mechanisms like the heme detoxification pathway. The conducted phenotypic screening of robenidine and 80 derivatives against newly transformed schistosomula and adult Schistosoma mansoni yielded 11 candidates with low EC50 values for newly transformed schistosomula (1.12-4.63\u00a0\u03bcM) and adults (2.78-9.47\u00a0\u03bcM). The structure-activity relationship revealed that electron-withdrawing groups at the phenyl moiety, as well as the presence of methyl groups adjacent to the guanidine moiety, enhanced the activity of derivatives against both stages of Schistosoma mansoni. The two compounds 2,2'-Bis[(3-cyano-4-fluorophenyl)methylene] carbonimidic Dihydrazide Hydrochloride (1) and 2,2'-Bis[(4-difluoromethoxyphenyl) ethylidene] carbonimidic Dihydrazide Hydrochloride (19), were selected for an in vivo study in Schistosoma mansoni-infected mice based on their potency, cytotoxicity, pharmacokinetic-, and physicochemical properties, but failed to reduce the worm burden significantly (worm burden reduction <20%). Thus, robenidine derivatives require further refinements to obtain higher antischistosomal specificity and in vivo activity.", "keywords": ["Male", "0301 basic medicine", "0303 health sciences", "Drug discovery", "Infectious and parasitic diseases", "RC109-216", "Schistosoma mansoni", "Structure-activity relationship", "Guanidines", "Article", "Schistosomiasis mansoni", "3. Good health", "Mice", "Schistosomicides", "Structure-Activity Relationship", "03 medical and health sciences", "Aminoguanidine", "Animals", "Female", "Robenidine derivative"], "contacts": [{"organization": "Christian N. Lotz, Alina Krollenbrock, Lea Imhof, Michael Riscoe, Jennifer Keiser,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/PMC11101930"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20for%20Parasitology%3A%20Drugs%20and%20Drug%20Resistance", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC11101930", "name": "item", "description": "PMC11101930", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC11101930"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-08-01T00:00:00Z"}}, {"id": "PMC5507504", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:27:08Z", "type": "Journal Article", "created": "2017-07-13", "title": "Organic farming enhances soil microbial abundance and activity\u2014A meta-analysis and meta-regression", "description": "Population growth and climate change challenge our food and farming systems and provide arguments for an increased intensification of agriculture. A promising option is eco-functional intensification through organic farming, an approach based on using and enhancing internal natural resources and processes to secure and improve agricultural productivity, while minimizing negative environmental impacts. In this concept an active soil microbiota plays an important role for various soil based ecosystem services such as nutrient cycling, erosion control and pest and disease regulation. Several studies have reported a positive effect of organic farming on soil health and quality including microbial community traits. However, so far no systematic quantification of whether organic farming systems comprise larger and more active soil microbial communities compared to conventional farming systems was performed on a global scale. Therefore, we conducted a meta-analysis on current literature to quantify possible differences in key indicators for soil microbial abundance and activity in organic and conventional cropping systems. All together we integrated data from 56 mainly peer-reviewed papers into our analysis, including 149 pairwise comparisons originating from different climatic zones and experimental duration ranging from 3 to more than 100 years. Overall, we found that organic systems had 32% to 84% greater microbial biomass carbon, microbial biomass nitrogen, total phospholipid fatty-acids, and dehydrogenase, urease and protease activities than conventional systems. Exclusively the metabolic quotient as an indicator for stresses on microbial communities remained unaffected by the farming systems. Categorical subgroup analysis revealed that crop rotation, the inclusion of legumes in the crop rotation and organic inputs are important farming practices affecting soil microbial community size and activity. Furthermore, we show that differences in microbial size and activity between organic and conventional farming systems vary as a function of land use (arable, orchards, and grassland), plant life cycle (annual and perennial) and climatic zone. In summary, this study shows that overall organic farming enhances total microbial abundance and activity in agricultural soils on a global scale.", "keywords": ["Crops", " Agricultural", "2. Zero hunger", "Organic Agriculture", "Science", "Climate", "Microbiota", "Q", "R", "Fabaceae", "04 agricultural and veterinary sciences", "15. Life on land", "13. Climate action", "Life Science", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Biomass", "Soil Microbiology", "Research Article"]}, "links": [{"href": "https://doi.org/PMC5507504"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC5507504", "name": "item", "description": "PMC5507504", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC5507504"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-07-12T00:00:00Z"}}, {"id": "PMC9846495", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:27:14Z", "type": "Journal Article", "created": "2023-01-04", "title": "A genome wide association study to dissect the genetic architecture of agronomic traits in Andean lupin (Lupinus mutabilis)", "description": "

Establishing Lupinus mutabilis as a protein and oil crop requires improved varieties adapted to EU climates. The genetic regulation of strategic breeding traits, including plant architecture, growing cycle length and yield, is unknown. This study aimed to identify associations between 16 669 single nucleotide polymorphisms (SNPs) and 9 agronomic traits on a panel of 223 L. mutabilis accessions, grown in four environments, by applying a genome wide association study (GWAS). Seven environment-specific QTLs linked to vegetative yield, plant height, pods number and flowering time, were identified as major effect QTLs, being able to capture 6 to 20% of the phenotypic variation observed in these traits. Furthermore, two QTLs across environments were identified for flowering time on chromosome 8. The genes FAF, GAMYB and LNK, regulating major pathways involved in flowering and growth habit, as well as GA30X1, BIM1, Dr1, HDA15, HAT3, interacting with these pathways in response to hormonal and environmental cues, were prosed as candidate genes. These results are pivotal to accelerate the development of L. mutabilis varieties adapted to European cropping conditions by using marker-assisted selection (MAS), as well as to provide a framework for further functional studies on plant development and phenology in this species.Maize is the world's second most important agricultural crop. The cereal was unknown to Europeans before the end of the 15th century, but since its arrival in Europe, it has changed agriculture, food and landscapes. Terraces where maize was cultivated in the Northwest of the Iberian Peninsula contributed to the formation of local cultures and identities. The history of maize and maize landscape are mementos that help to recover traditional practices, fostering identities, and are crucial for the successful implementation of sustainable policies to provide prosperous futures.

Summary

Maize (Zea mays L.) in the Iberian Peninsula embodies a history of landscape changes where the concepts of \uffe2\uff80\uff98exotic\uffe2\uff80\uff99, \uffe2\uff80\uff98traditional\uffe2\uff80\uff99 and \uffe2\uff80\uff98hybrid\uffe2\uff80\uff99 help to understand the engagements between landscape, farmers, agronomists (since the 19th and 20th centuries) and seeds. Today, landscapes reveal biophysical and ecological changes that reflect a panoply of intentions. A multitude of agents, and their interactions, acted upon those territories over time.

Using historical sources from the leading institutions dedicated to agricultural research in the Iberian Peninsula, this paper aims to (1) contribute to a better understanding of the maize landscape and culture in the Iberian Peninsula and (2) interrogate how landscape changes (and the landscape history of maize) can frame local or regional heritage and identities reflecting customs or ways of life.

The analysis unveils networks of knowledge, agricultural technologies and seed exchange. Politicians, economists, engineers, agronomists, farmers, governmental officials and agricultural industries planned and transformed traditional rural practices into modern and industrialised ones. Experts and politicians, willing to improve agricultural practices and seeds, using hybrid seeds or building new irrigation systems, led to deep social and landscape changes, allowing maize to cover territories far away from its traditional domains. Moreover, despite farmers' resistance, hybrid maize substituted landraces, eroding agrobiodiversity. Nowadays, the south and east regions of the Iberian Peninsula are the main producers of maize (hybrid), whereas in the Northwest maize is an occasional crop, being replaced by vineyards for economic reasons.

Background: Water and nitrogen (N) are essential resources influencing plant growth and yield. To improve their efficiencies in crop production is challenging because the physiological mechanisms of water and N coupling and their interactive effect on crop water use efficiency (WUE) are not well understood yet.

Aim: The aim of this study was to investigate the physiological responses and phytohormones signaling in oats in response to soil water status and N supply under fertigation, to explore the mechanisms regulating plant growth and WUE.

Methods: Oat plants were subjected to the factorial combination of three soil moisture regimes (50, 70, and 90% of soil water holding capacity, SWHC) and three N levels (fertilized with 74, 149, and 298 mg kg\uffe2\uff88\uff921).

Results: The stomatal conductance (gs) was significantly decreased by soil water deficit, and also by the highest N level, whereas photosynthesis rate (An) was unaffected by neither water nor N. Consequently, intrinsic WUE (WUEint, An/gs) was highest under reduced irrigation and high N fertilization. This effect at stomatal level was affirmed by responses in whole plant WUE (WUEb), which was positively correlated with shoot \uffce\uffb413C. A positive correlation between \uffce\uffb418O and \uffce\uffb413C in shoots further indicated that decreases of gs rather than changes in An contributed to the enhanced WUE.

Conclusion: Moderate soil water deficit and sufficient N supply is recommended for saving irrigation water and improving WUE on fertigated oat plants without compromising biomass accumulation to any large extent.Broadband topological metamaterials hold the key for designing the next generation of integrated photonic platforms and microwave devices given their protected back\uffe2\uff80\uff90scattering\uffe2\uff80\uff90free and unidirectional edge states, among other exotic properties. However, synthesizing such metamaterial has proven challenging. Here, a broadband bandgap (relative bandwidth of more than 43%) Valley\uffe2\uff80\uff90Hall topological metamaterial with deep subwavelength thickness is proposed. The present topological metamaterial is composed of three layers printed circuit boards whose total thickness is 1.524\uffc2\uffa0mm \uffe2\uff89\uff88 \uffce\uffbb/100. The topological phase transition is achieved by introducing an asymmetry parameter \uffce\uffb4r. Three mechanically reconfigurable edge states can be obtained by varying interlayer displacement. Their robust transmission is demonstrated through two kinds of waveguide domain walls with cavities and disorders. Exploiting the proposed topological metamaterial, a six\uffe2\uff80\uff90way power divider is constructed and measured as a proof\uffe2\uff80\uff90of\uffe2\uff80\uff90concept of the potential of the proposed technology for future electromagnetic devices.Haloalkane dehalogenases (HLDs) are \uffce\uffb1/\uffce\uffb2\uffe2\uff80\uff90hydrolases that convert halogenated compounds to their corresponding alcohols. The overall kinetic mechanism proceeds via four steps: (i) binding of halogenated substrate, (ii) bimolecular nucleophilic substitution (SN2) leading to the cleavage of a carbon\uffe2\uff80\uff90halogen bond and the formation of an alkyl\uffe2\uff80\uff90enzyme intermediate, (iii) nucleophilic addition of a water molecule resulting in the hydrolysis of the intermediate to the corresponding alcohol and (iv) release of the reaction products \uffe2\uff80\uff93 an alcohol, a halide ion and a proton. Although, the overall reaction has been reported as irreversible, several kinetic evidences from previous studies suggest the reversibility of the first SN2 chemical step. To study this phenomenon, we have engineered HLDs to stop the catalytic cycle at the stage of the alkyl\uffe2\uff80\uff90enzyme intermediate. The ability of the intermediate to exchange halides was confirmed by a stopped\uffe2\uff80\uff90flow fluorescence binding analysis. Finally, the transhalogenation reaction was confirmed with several HLDs and 2,3\uffe2\uff80\uff90dichloropropene in the presence of a high concentration of iodide. The formation of the transhalogenation product 3\uffe2\uff80\uff90iodo\uffe2\uff80\uff902\uffe2\uff80\uff90chloropropene catalysed by five mutant HLDs was identified by gas chromatography coupled with mass spectrometry. Hereby we demonstrated the reversibility of the cleavage of the carbon\uffe2\uff80\uff90halogen bond by HLDs resulting in a transhalogenation. After optimization, the transhalogenation reaction can possibly find its use in biocatalytic applications. Enabling this reaction by strategically engineering the enzyme to stop at an intermediate in the catalytic cycle that is synthetically more useful than the product of the natural pathway is a novel concept.

magnified image

", "keywords": ["0301 basic medicine", "enzyme catalysis; halide exchange; haloalkane dehalogenase; nucleophilic substitution; redirection of reaction; transhalogenation", "0303 health sciences", "03 medical and health sciences"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/adsc.201900132"}, {"href": "https://doi.org/10.1002/adsc.201900132"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Advanced%20Synthesis%20%26amp%3B%20Catalysis", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/adsc.201900132", "name": "item", "description": "10.1002/adsc.201900132", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/adsc.201900132"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-17T00:00:00Z"}}, {"id": "10.1002/advs.201901408", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:00Z", "type": "Journal Article", "created": "2019-09-19", "title": "Natural Microbial Communities Can Be Manipulated by Artificially Constructed Biofilms", "description": "Abstract

Biofouling proceeds in successive steps where the primary colonizers affect the phylogenetic and functional structure of a future microbial consortium. Using microbiologically influenced corrosion (MIC) as a study case, a novel approach for material surface protection is described, which does not prevent biofouling, but rather shapes the process of natural biofilm development to exclude MIC\uffe2\uff80\uff90related microorganisms. This approach interferes with the early steps of natural biofilm formation affecting how the community is finally developed. It is based on a multilayer artificial biofilm, composed of electrostatically modified bacterial cells, producing antimicrobial compounds, extracellular antimicrobial polyelectrolyte matrix, and a water\uffe2\uff80\uff90proof rubber elastomer barrier. The artificial biofilm is constructed layer\uffe2\uff80\uff90by\uffe2\uff80\uff90layer (LBL) by manipulating the electrostatic interactions between microbial cells and material surfaces. Field testing on standard steel coupons exposed in the sea for more than 30 days followed by laboratory analyses using molecular\uffe2\uff80\uff90biology tools demonstrate that the preapplied artificial biofilm affects the phylogenetic structure of the developing natural biofilm, reducing phylogenetic diversity and excluding MIC\uffe2\uff80\uff90related bacteria. This sustainable solution for material protection showcases the usefulness of artificially guiding microbial evolutionary processes via the electrostatic modification and controlled delivery of bacterial cells and extracellular matrix to the exposed material surfaces.Gypsum soils in the Mediterranean Basin house large numbers of edaphic specialists that are adapted to stressful environments. The evolutionary history and standing genetic variation of these taxa have been influenced by the geological and paleoclimatic complexity of this area and the long\uffe2\uff80\uff90standing effect of human activities. However, little is known about the origin of Mediterranean gypsophiles and the factors affecting their genetic diversity and population structure.

METHODS

Using phylogenetic and phylogeographic approaches based on microsatellites and sequence data from nuclear and chloroplast regions, we evaluated the divergence time, genetic diversity, and population structure of 27 different populations of the widespread Iberian gypsophile Lepidium subulatum throughout its entire geographic range.

RESULTS

Lepidium subulatum diverged from its nearest relatives ~3 million years ago, and ITS and psbA/matK trees supported the monophyly of the species. These results suggest that both geological and climatic changes in the region around the Plio\uffe2\uff80\uff90Pleistocene promoted its origin, compared to other evolutionary processes. We found high genetic diversity in both nuclear and chloroplast markers, but a greater population structure in the chloroplast data. These results suggest that while seed dispersal is limited, pollen flow may be favored by the presence of numerous habitat patches that enhance the movement of pollinators.

CONCLUSIONS

Despite being an edaphic endemic, L. subulatum possesses high genetic diversity probably related to its relatively old age and high population sizes across its range. Our study highlights the value of using different markers to fully understand the phylogeographic history of plant species.There is an urgent need to develop viable, renewable, sustainable energy systems that can reduce global dependence on fossil fuel sources of energy. Biofuels such as ethanol are being utilized as blends in surface transportation fuels and have the potential to improve sustainability and reduce greenhouse gas emissions in the short term. Bioethanol, the most widely used liquid biofuel, is currently produced by converting sugars or starches from feed crops into ethanol. Use of this fuel source displaces and draws water consumption away from agricultural crops, increases soil erosion by shifting land from perennial grasses to annual crops, and increases use of fertilizers and insecticides. In contrast, bioethanol made from lignocellulosic biomass feedstocks does not have these limitations and in addition, offers a larger resource base: the amount of cellulosic material available for potential use vastly outweighs the amount of available starch\uffe2\uff80\uff90based feedstock. Therefore, bioethanol from lignocellulosic biomass has attracted considerable interest from biofuel developers. This review is an update of some developments to optimize cellulose extraction from feedstock crops and to improve crop yields and logistics. It concludes that agricultural and forestry systems that incorporate lignocellulosic biomass crops can be designed for improved ecological function and energy use efficiency. Development of crops that have both desirable cell\uffe2\uff80\uff90wall traits and high biomass productivity under sustainable low\uffe2\uff80\uff90input conditions can significantly enhance the economics and efficiency of the conversion process. Optimizing the logistics of moving feedstock from field or forest to bio\uffe2\uff80\uff90refinery can significantly reduce costs of using lignocellulosic feedstocks. \uffc2\uffa9 2013 Society of Chemical Industry and John Wiley & Sons, Ltd

", "keywords": ["0301 basic medicine", "2. Zero hunger", "03 medical and health sciences", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "04 agricultural and veterinary sciences", "15. Life on land", "7. Clean energy", "12. Responsible consumption"]}, "links": [{"href": "https://doi.org/10.1002/bbb.1418"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biofuels%2C%20Bioproducts%20and%20Biorefining", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/bbb.1418", "name": "item", "description": "10.1002/bbb.1418", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/bbb.1418"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-07-09T00:00:00Z"}}, {"id": "10.1002/bbb.325", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:01Z", "type": "Journal Article", "created": "2011-09-01", "title": "Biofuels: Ethics And Policy-Making", "description": "Abstract

Mandatory targets for biofuels have led to their rapid global adoption, but ethical problems with their large\uffe2\uff80\uff90scale production are widely reported. Research is underway to find new biofuel technologies that mitigate climate change and can be produced sustainably and economically. Following an 18\uffe2\uff80\uff90month inquiry, the Nuffield Council on Bioethics has developed an ethical framework for biofuels policy\uffe2\uff80\uff90making. Based on a number of widely held ethical values, six ethical principles are introduced that should be considered in biofuels policy\uffe2\uff80\uff90making. Many current biofuels policies fail this ethical \uffe2\uff80\uff98test\uffe2\uff80\uff99. An overarching ethical standard for biofuels is proposed that includes the protection of human rights and the environment, full life cycle assessment of greenhouse gas emissions, trade principles that are fair, access and benefit\uffe2\uff80\uff90sharing schemes to encourage equitable distribution of costs and benefits, and instruments to incentivize the development of promising new approaches. The Council goes on to make recommendations for changes to current policy. \uffc2\uffa9 2011 Society of Chemical Industry and John Wiley & Sons, Ltd

", "keywords": ["0301 basic medicine", "03 medical and health sciences", "13. Climate action", "01 natural sciences", "7. Clean energy", "0105 earth and related environmental sciences", "12. Responsible consumption", "3. Good health"], "contacts": [{"organization": "Joyce Tait, Alena Buyx,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1002/bbb.325"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biofuels%2C%20Bioproducts%20and%20Biorefining", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/bbb.325", "name": "item", "description": "10.1002/bbb.325", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/bbb.325"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2011-08-25T00:00:00Z"}}, {"id": "10.1002/biot.202000165", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:01Z", "type": "Journal Article", "created": "2020-10-21", "title": "Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates", "description": "Abstract

Growing environmental concern sparks renewed interest in the sustainable production of (bio)materials that can replace oil\uffe2\uff80\uff90derived goods. Polyhydroxyalkanoates (PHAs) are isotactic polymers that play a critical role in the central metabolism of producer bacteria, as they act as dynamic reservoirs of carbon and reducing equivalents. PHAs continue to attract industrial attention as a starting point toward renewable, biodegradable, biocompatible, and versatile thermoplastic and elastomeric materials. Pseudomonas species have been known for long as efficient biopolymer producers, especially for medium\uffe2\uff80\uff90chain\uffe2\uff80\uff90length PHAs. The surge of synthetic biology and metabolic engineering approaches in recent years offers the possibility of exploiting the untapped potential of Pseudomonas cell factories for the production of tailored PHAs. In this article, an overview of the metabolic and regulatory circuits that rule PHA accumulation in Pseudomonas putida is provided, and approaches leading to the biosynthesis of novel polymers (e.g., PHAs including nonbiological chemical elements in their structures) are discussed. The potential of novel PHAs to disrupt existing and future market segments is closer to realization than ever before. The review is concluded by pinpointing challenges that currently hinder the wide adoption of bio\uffe2\uff80\uff90based PHAs, and strategies toward programmable polymer biosynthesis from alternative substrates in engineered P. putida strains are proposed.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Biopolymer", "PHA", "Pseudomonas putida", "Polyhydroxyalkanoates", "Carbon", "12. Responsible consumption", "03 medical and health sciences", "/dk/atira/pure/sustainabledevelopmentgoals/affordable_and_clean_energy; name=SDG 7 - Affordable and Clean Energy", "Metabolic Engineering", "Pseudomonas", "Pathway engineering", "Metabolic engineering", "Synthetic biology"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.202000165"}, {"href": "https://doi.org/10.1002/biot.202000165"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biotechnology%20Journal", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/biot.202000165", "name": "item", "description": "10.1002/biot.202000165", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/biot.202000165"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-11-09T00:00:00Z"}}, {"id": "10.1002/cbic.202000051", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:01Z", "type": "Journal Article", "created": "2020-01-31", "title": "An Engineered E.\u2005coli Strain for Direct in Vivo Fluorination", "description": "Abstract

Selectively fluorinated compounds are found frequently in pharmaceutical and agrochemical products where currently 25\uffe2\uff80\uff9330\uffe2\uff80\uff89% of optimised compounds emerge from development containing at least one fluorine atom. There are many methods for the site\uffe2\uff80\uff90specific introduction of fluorine, but all are chemical and they often use environmentally challenging reagents. Biochemical processes for C\uffe2\uff88\uff92F bond formation are attractive, but they are extremely rare. In this work, the fluorinase enzyme, originally identified from the actinomycete bacterium Streptomyces cattleya, is engineered into Escherichia coli in such a manner that the organism is able to produce 5\uffe2\uff80\uffb2\uffe2\uff80\uff90fluorodeoxyadenosine (5\uffe2\uff80\uffb2\uffe2\uff80\uff90FDA) from S\uffe2\uff80\uff90adenosyl\uffe2\uff80\uff90l\uffe2\uff80\uff90methionine (SAM) and fluoride in live E.\uffe2\uff80\uff85coli cells. Success required the introduction of a SAM transporter and deletion of the endogenous fluoride efflux capacity in order to generate an E.\uffe2\uff80\uff85coli host that has the potential for future engineering of more elaborate fluorometabolites.

", "keywords": ["SAM transporters", "0301 basic medicine", "570", "S-Adenosylmethionine", "0303 health sciences", "Deoxyadenosines", "Halogenation", "DAS", "Fluorine", "Halogenations", "540", "QD Chemistry", "Streptomyces", "3. Good health", "03 medical and health sciences", "Bacterial Proteins", "Isomerism", "Escherichia coli", "QD", "Fluoride channels", "Genetic Engineering", "Oxidoreductases", "Fluorinases"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/cbic.202000051"}, {"href": "https://doi.org/10.1002/cbic.202000051"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/ChemBioChem", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/cbic.202000051", "name": "item", "description": "10.1002/cbic.202000051", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/cbic.202000051"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-03-03T00:00:00Z"}}, {"id": "10.1002/ece3.1867", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:02Z", "type": "Journal Article", "created": "2016-01-11", "title": "Grazing Exclusion Reduced Soil Respiration But Increased Its Temperature Sensitivity In A Meadow Grassland On The Tibetan Plateau", "description": "Abstract

Understanding anthropogenic influences on soil respiration (Rs) is critical for accurate predictions of soil carbon fluxes, but it is not known how Rs responds to grazing exclusion (GE). Here, we conducted a manipulative experiment in a meadow grassland on the Tibetan Plateau to investigate the effects of GE on Rs. The exclusion of livestock significantly increased soil moisture and above\uffe2\uff80\uff90ground biomass, but it decreased soil temperature, microbial biomass carbon (MBC), and Rs. Regression analysis indicated that the effects of GE on Rs were mainly due to changes in soil temperature, soil moisture, and MBC. Compared with the grazed blocks, GE significantly decreased soil carbon release by 23.6% over the growing season and 21.4% annually, but it increased the temperature sensitivity (Q10) of Rs by 6.5% and 14.2% for the growing season and annually respectively. Therefore, GE may reduce the release of soil carbon from the Tibetan Plateau, but under future climate warming scenarios, the increases in Q10 induced by GE could lead to increased carbon emissions.

", "keywords": ["570", "MICROBIAL RESPIRATION", "Environmental Sciences & Ecology", "Plant Productivity", "Temperature Sensitivity", "ALPINE GRASSLAND", "630", "Microbial Biomass Carbon", "NORTHERN CHINA", "SEASONAL PATTERNS", "MOUNTAIN GRASSLANDS", "Grazing Exclusion", "Tibetan Plateau", "PLANT-COMMUNITIES", "Original Research", "2. Zero hunger", "Science & Technology", "CLIMATE-CHANGE", "CO2 EFFLUX", "Ecology", "04 agricultural and veterinary sciences", "15. Life on land", "INNER-MONGOLIA", "BELOW-GROUND BIOMASS", "Soil Respiration", "13. Climate action", "0401 agriculture", " forestry", " and fisheries", "Life Sciences & Biomedicine"]}, "links": [{"href": "https://doi.org/10.1002/ece3.1867"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.1867", "name": "item", "description": "10.1002/ece3.1867", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.1867"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-01-11T00:00:00Z"}}, {"id": "10.1002/ece3.1879", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:02Z", "type": "Journal Article", "created": "2016-01-22", "title": "Effects Of Fertilization On Microbial Abundance And Emissions Of Greenhouse Gases (Ch4 And N2o) In Rice Paddy Fields", "description": "Abstract

This study is to explore effects of nitrogen application and straw incorporation on abundance of relevant microbes and CH4 and N2O fluxes in a midseason aerated rice paddy field. Fluxes of CH4 and N2O were recorded, and abundance of relevant soil microbial functional genes was determined during rice\uffe2\uff80\uff90growing season in a 6\uffe2\uff80\uff90year\uffe2\uff80\uff90long fertilization experiment field in China. Results indicate that application of urea significantly changed the functional microbial composition, while the influence of straw incorporation was not significant. Application of urea significantly decreased the gene abundances of archaeal amoA and mcrA, but it significantly increased the gene abundances of bacterial amoA. CH4 emission was significantly increased by fresh straw incorporation. Incorporation of burnt straw tended to increase CH4 emission, while the urea application had no obvious effect on CH4 emission. N2O emission was significantly increased by urea application, while fresh or burnt straw incorporation tended to decrease N2O emission. The functional microbial composition did not change significantly over time, although the abundances of pmoA, archaeal amoA, nirS, and nosZ genes changed significantly. The change of CH4 emission showed an inverse trend with the one of the N2O emissions over time. To some extent, the abundance of some functional genes in this study can explain CH4 and N2O emissions. However, the correlation between CH4 and N2O emissions and the abundance of related functional genes was not significant. Environmental factors, such as soil Eh, may be more related to CH4 and N2O emissions.

", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "03 medical and health sciences", "13. Climate action", "15. Life on land", "Original Research"]}, "links": [{"href": "https://doi.org/10.1002/ece3.1879"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.1879", "name": "item", "description": "10.1002/ece3.1879", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.1879"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-01-22T00:00:00Z"}}, {"id": "10.1002/ecy.1513", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:03Z", "type": "Journal Article", "created": "2016-07-02", "title": "Land Use Intensification In The Humid Tropics Increased Both Alpha And Beta Diversity Of Soil Bacteria", "description": "Abstract

Anthropogenic pressures on tropical forests are rapidly intensifying, but our understanding of their implications for biological diversity is still very limited, especially with regard to soil biota, and in particular soil bacterial communities. Here we evaluated bacterial community composition and diversity across a gradient of land use intensity in the eastern Amazon from undisturbed primary forest, through primary forests varyingly disturbed by fire, regenerating secondary forest, pasture, and mechanized agriculture. Soil bacteria were assessed by paired\uffe2\uff80\uff90end Illumina sequencing of 16S rRNA gene fragments (V4 region). The resulting sequences were clustered into operational taxonomic units (OTU) at a 97% similarity threshold. Land use intensification increased the observed bacterial diversity (both OTU richness and community heterogeneity across space) and this effect was strongly associated with changes in soil pH. Moreover, land use intensification and subsequent changes in soil fertility, especially pH, altered the bacterial community composition, with pastures and areas of mechanized agriculture displaying the most contrasting communities in relation to undisturbed primary forest. Together, these results indicate that tropical forest conversion impacts soil bacteria not through loss of diversity, as previously thought, but mainly by imposing marked shifts on bacterial community composition, with unknown yet potentially important implications for ecological functions and services performed by these communities.

", "keywords": ["Rios de composi\u00e7\u00e3o de comunidade bacteriana", "2. Zero hunger", "0301 basic medicine", "570", "0303 health sciences", "550", "Bacteria", "Biodiversidade subterr\u00e2nea", "Agriculture", "Biodiversity", "Forests", "15. Life on land", "Below\u2010ground biodiversity", "High\u2010throughput sequencing", "Soil", "03 medical and health sciences", "RNA", " Ribosomal", " 16S", "Sequenciamento de alto rendimento", "Rivers of bacterial community composition", "Soil Microbiology"]}, "links": [{"href": "https://eprints.lancs.ac.uk/id/eprint/82660/1/de_Carvalho_et_al_2016_raw_pdf.pdf"}, {"href": "https://doi.org/10.1002/ecy.1513"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ecy.1513", "name": "item", "description": "10.1002/ecy.1513", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ecy.1513"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-09-09T00:00:00Z"}}, {"id": "10.1002/ece3.5244", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:02Z", "type": "Journal Article", "created": "2019-06-10", "title": "Evolution of interdisciplinarity in biodiversity science", "description": "Abstract

The study of biodiversity has grown exponentially in the last thirty years in response to demands for greater understanding of the function and importance of Earth's biodiversity and finding solutions to conserve it. Here, we test the hypothesis that biodiversity science has become more interdisciplinary over time. To do so, we analyze 97,945 peer\uffe2\uff80\uff90reviewed articles over a twenty\uffe2\uff80\uff90two\uffe2\uff80\uff90year time period (1990\uffe2\uff80\uff932012) with a continuous time dynamic model, which classifies articles into concepts (i.e., topics and ideas) based on word co\uffe2\uff80\uff90occurrences. Using the model output, we then quantify different aspects of interdisciplinarity: concept diversity, that is, the diversity of topics and ideas across subdisciplines in biodiversity science, subdiscipline diversity, that is, the diversity of subdisciplines across concepts, and network structure, which captures interactions between concepts and subdisciplines. We found that, on average, concept and subdiscipline diversity in biodiversity science were either stable or declining, patterns which were driven by the persistence of rare concepts and subdisciplines and a decline in the diversity of common concepts and subdisciplines, respectively. Moreover, our results provide evidence that conceptual homogenization, that is, decreases in temporal \uffce\uffb2 concept diversity, underlies the observed trends in interdisciplinarity. Together, our results reveal that biodiversity science is undergoing a dynamic phase as a scientific discipline that is consolidating around a core set of concepts. Our results suggest that progress toward addressing the biodiversity crisis via greater interdisciplinarity during the study period may have been slowed by extrinsic factors, such as the failure to invest in research spanning across concepts and disciplines. However, recent initiatives such as the Intergovernmental Science\uffe2\uff80\uff90Policy Platform on Biodiversity and Ecosystem Services (IPBES) may attract broader support for biodiversity\uffe2\uff80\uff90related issues and hence interdisciplinary approaches to address scientific, political, and societal challenges in the coming years.

", "keywords": ["0301 basic medicine", "bibliographic analysis", "topic models", "0303 health sciences", "03 medical and health sciences", "conceptual homogenization", "interdisciplinarity", "Ecology", "13. Climate action", "15. Life on land", "Academic Practice in Ecology and Evolution", "QH540-549.5", "biodiversity"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.5244"}, {"href": "https://doi.org/10.1002/ece3.5244"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.5244", "name": "item", "description": "10.1002/ece3.5244", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.5244"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-06-01T00:00:00Z"}}, {"id": "10.1002/ece3.6547", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:02Z", "type": "Journal Article", "created": "2020-07-09", "title": "The distribution of herbivores between leaves matches their performance only in the absence of competitors", "description": "Abstract

Few studies have tested how plant quality and the presence of competitors interact in determining how herbivores choose between different leaves within a plant. We investigated this in two herbivorous spider mites sharing tomato plants: Tetranychus urticae, which generally induces plant defenses, and Tetranychus evansi, which suppresses them, creating asymmetrical effects on coinfesting competitors. On uninfested plants, both herbivore species preferred young leaves, coinciding with increased mite performance. On plants with heterospecifics, the mites did not prefer leaves on which they had a better performance. In particular, T.\uffc2\uffa0urticae avoided leaves infested with T.\uffc2\uffa0evansi, which is in agreement with T.\uffc2\uffa0urticae being outcompeted by T.\uffc2\uffa0evansi. In contrast, T.\uffc2\uffa0evansi did not avoid leaves with the other species, but distributed itself evenly over plants infested with heterospecifics. We hypothesize that this behavior of T.\uffc2\uffa0evansi may prevent further spread of T.\uffc2\uffa0urticae over the shared plant. Our results indicate that leaf age determines within\uffe2\uff80\uff90plant distribution of herbivores only in absence of competitors. Moreover, they show that this distribution depends on the order of arrival of competitors and on their effects on each other, with herbivores showing differences in behavior within the plant as a possible response to the outcome of those interactions.Long amplicon metabarcoding has opened the door for phylogenetic analysis of the largely unknown communities of microeukaryotes in soil. Here, we amplified and sequenced the ITS and LSU regions of the rDNA operon (around 1500\uffc2\uffa0bp) from grassland soils using PacBio SMRT sequencing. We tested how three different methods for generation of operational taxonomic units (OTUs) effected estimated richness and identified taxa, and how well large\uffe2\uff80\uff90scale ecological patterns associated with shifting environmental conditions were recovered in data from the three methods. The field site at Kungs\uffc3\uffa4ngen Nature Reserve has drawn frequent visitors since Linnaeus's time, and its species rich vegetation includes the largest population of Fritillaria meleagris in Sweden. To test the effect of different OTU generation methods, we sampled soils across an abrupt moisture transition that divides the meadow community into a Carex acuta dominated plant community with low species richness in the wetter part, which is visually distinct from the mesic\uffe2\uff80\uff90dry part that has a species rich grass\uffe2\uff80\uff90dominated plant community including a high frequency of F.\uffc2\uffa0meleagris. We used the moisture and plant community transition as a framework to investigate how detected belowground microeukaryotic community composition was influenced by OTU generation methods. Soil communities in both moisture regimes were dominated by protists, a large fraction of which were taxonomically assigned to Ciliophora (Alveolata) while 30%\uffe2\uff80\uff9340% of all reads were assigned to kingdom Fungi. Ecological patterns were consistently recovered irrespective of OTU generation method used. However, different methods strongly affect richness estimates and the taxonomic and phylogenetic resolution of the characterized community with implications for how well members of the microeukaryotic communities can be recognized in the data.

", "keywords": ["580", "species hypothesis", "Ekologi", "0301 basic medicine", "0303 health sciences", "Ecology", "rDNA", "Biological Systematics", "15. Life on land", "03 medical and health sciences", "14. Life underwater", "ITS", "Research Articles", "clustering"]}, "links": [{"href": "https://pub.epsilon.slu.se/27699/1/eshghi-sahraei-s-et-al-220505.pdf"}, {"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/ece3.8676"}, {"href": "https://doi.org/10.1002/ece3.8676"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecology%20and%20Evolution", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ece3.8676", "name": "item", "description": "10.1002/ece3.8676", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ece3.8676"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-03-01T00:00:00Z"}}, {"id": "10.1002/ecs2.2226", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:03Z", "type": "Journal Article", "created": "2018-05-31", "title": "Mycorrhiza in tree diversity-ecosystem function relationships: conceptual framework and experimental implementation", "description": "Abstract

The widely observed positive relationship between plant diversity and ecosystem functioning is thought to be substantially driven by complementary resource use of plant species. Recent work suggests that biotic interactions among plants and between plants and soil organisms drive key aspects of resource use complementarity. Here, we provide a conceptual framework for integrating positive biotic interactions across guilds of organisms, more specifically between plants and mycorrhizal types, to explain resource use complementarity in plants and its consequences for plant competition. Our overarching hypothesis is that ecosystem functioning increases when more plant species associate with functionally dissimilar mycorrhizal fungi because differing mycorrhizal types will increase coverage of habitat space for and reduce competition among plants. We introduce a recently established field experiment (MyDiv) that uses different pools of tree species that associate with either arbuscular or ectomycorrhizal fungi to create orthogonal experimental gradients in tree species richness and mycorrhizal associations and present initial results. Finally, we discuss options for future mechanistic studies on resource use complementarity within MyDiv. We show how mycorrhizal types and biotic interactions in MyDiv can be used in the future to test novel questions regarding the mechanisms underlying biodiversity\uffe2\uff80\uff93ecosystem function relationships.

", "keywords": ["0106 biological sciences", "0301 basic medicine", "2. Zero hunger", "biodiversity\u2013ecosystem functioning", "experimental design", "Ecology", "arbuscular mycorrhiza", "15. Life on land", "01 natural sciences", "ectomycorrhiza", "Article", "biotic interactions", "03 medical and health sciences", "biodiversity effects", "QH540-549.5"]}, "links": [{"href": "https://esajournals.onlinelibrary.wiley.com/doi/pdf/10.1002/ecs2.2226"}, {"href": "https://doi.org/10.1002/ecs2.2226"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecosphere", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1002/ecs2.2226", "name": "item", "description": "10.1002/ecs2.2226", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1002/ecs2.2226"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-05-01T00:00:00Z"}}, {"id": "10.1002/ecs2.2645", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-24T16:14:03Z", "type": "Journal Article", "created": "2019-03-19", "title": "Uneven global distribution of food web studies under climate change", "description": "Abstract

Trophic interactions within food webs affect species distributions, coexistence, and provision of ecosystem services but can be strongly impacted by climatic changes. Understanding these impacts is therefore essential for managing ecosystems and sustaining human well\uffe2\uff80\uff90being. Here, we conducted a global synthesis of terrestrial, marine, and freshwater studies to identify key gaps in our knowledge of climate change impacts on food webs and determine whether the areas currently studied are those most likely to be impacted by climate change. We found research suffers from a strong geographic bias, with only 3.5% of studies occurring in the tropics. Importantly, the distribution of sites sampled under projected climate changes was biased\uffe2\uff80\uff94areas with decreases or large increases in precipitation and areas with low magnitudes of temperature change were under\uffe2\uff80\uff90represented. Our results suggest that understanding of climate change impacts on food webs could be broadened by considering more than two trophic levels, responses in addition to species abundance and biomass, impacts of a wider suite of climatic variables, and tropical ecosystems. Most importantly, to enable better forecasts of biodiversity responses to climate change, we identify critically under\uffe2\uff80\uff90represented geographic regions and climatic conditions which should be prioritized in future research.Changes in substrate quality driven by climate, land use, or other forms of global change may represent a strong selective force on microbial communities. Invasion of new taxa into a community through dispersal, evolution, or recolonization could impact the outcome of this environmental selection. Here, we simulated substrate change with a trait\uffe2\uff80\uff90based model of microbial litter decomposition (DEMENTpy) to assess the legacy effects of past substrate quality and the impact of selection by a new substrate on community decomposition activity. Simulations were run with different levels of invasion, including invasion from communities long\uffe2\uff80\uff90adapted to the new substrate. Legacy effects were evident with substrate change for native communities differing in composition. Protein was the only substrate that exerted a strong enough selective force to affect community composition. Legacy effects disappeared when invaders came from substrates similar to the new substrate. Together, our simulations demonstrate that substrate quality changes associated with global change can lead to legacy effects on substrate degradation. In decomposing plant litter, such legacy effects can occur if substrate inputs shift to higher protein content and if invasion is low.