{"type": "FeatureCollection", "features": [{"id": "10.1007/s00248-016-0730-z", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:14:30Z", "type": "Journal Article", "created": "2016-02-02", "title": "Responses Of Soil Bacterial Communities To Nitrogen Deposition And Precipitation Increment Are Closely Linked With Aboveground Community Variation", "description": "It has been predicted that precipitation and atmospheric nitrogen (N) deposition will increase in northern China; yet, ecosystem responses to the interactive effects of water and N remain largely unknown. In particular, responses of belowground microbial community to projected global change and their potential linkages to aboveground macro-organisms are rarely studied. In this study, we examined the responses of soil bacterial diversity and community composition to increased precipitation and multi-level N deposition in a temperate steppe in Inner Mongolia, China, and explored the diversity linkages between aboveground and belowground communities. It was observed that N addition caused the significant decrease in bacterial alpha-diversity and dramatic changes in community composition. In addition, we documented strong correlations of alpha- and beta-diversity between plant and bacterial communities in response to N addition. It was found that N enriched the so-called copiotrophic bacteria, but reduced the oligotrophic groups, primarily by increasing the soil inorganic N content and carbon availability and decreasing soil pH. We still highlighted that increased precipitation tended to alleviate the effects of N on bacterial diversity and dampen the plant-microbe connections induced by N. The counteractive effects of N addition and increased precipitation imply that even though the ecosystem diversity and function are predicted to be negatively affected by N deposition in the coming decades; the combination with increased precipitation may partially offset this detrimental effect.", "keywords": ["0301 basic medicine", "2. Zero hunger", "China", "0303 health sciences", "Bacteria", "Nitrogen", "Climate", "Microbial Consortia", "Water", "Biodiversity", "Plants", "15. Life on land", "Carbon", "6. Clean water", "Soil", "03 medical and health sciences", "13. Climate action", "Chemical Precipitation", "Ecosystem", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1007/s00248-016-0730-z"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microbial%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1007/s00248-016-0730-z", "name": "item", "description": "10.1007/s00248-016-0730-z", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1007/s00248-016-0730-z"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2016-02-02T00:00:00Z"}}, {"id": "10.1016/j.biortech.2018.09.044", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:15:50Z", "type": "Journal Article", "created": "2018-09-10", "title": "Biokinetics of microbial consortia using biogenic sulfur as a novel electron donor for sustainable denitrification", "description": "In this study, the biokinetics of autotrophic denitrification with biogenic S0 (ADBIOS) for the treatment of nitrogen pollution in wastewaters were investigated. The used biogenic S0, a by-product of gas desulfurization, was an elemental microcrystalline orthorhombic sulfur with a median size of 4.69\u202f\u00b5m and a specific surface area of 3.38\u202fm2/g, which made S0 particularly reactive and bioavailable. During denitritation, the biomass enriched on nitrite (NO2-) was capable of degrading up to 240\u202fmg/l NO2--N with a denitritation activity of 339.5\u202fmg NO2--N/g VSS\u00b7d. The use of biogenic S0 induced a low NO2--N accumulation, hindering the NO2--N negative impact on the denitrifying consortia and resulting in a specific denitrification activity of 223.0\u202fmg NO3--N/g VSS\u00b7d. Besides Thiobacillus being the most abundant genus, Moheibacter and Thermomonas were predominantly selected for denitrification and denitritation, respectively.", "keywords": ["Nitrite accumulation", "Nitrogen", "Microbial Consortia", "Biokinetics", "0211 other engineering and technologies", "Electrons", "02 engineering and technology", "Autotrophic denitrification", "Thiobacillus", "01 natural sciences", "6. Clean water", "Community structure", "12. Responsible consumption", "Kinetics", "Bioreactors", "13. Climate action", "Autotrophic denitrification; Biogenic sulfur; Nitrite accumulation; Biokinetics; Community structure", "Biogenic sulfur", "Denitrification", "Biomass", "Sulfur", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://eprints.gla.ac.uk/168662/1/168662.pdf"}, {"href": "https://www.iris.unina.it/bitstream/11588/722336/1/2018%20-%20Kostrytsia%20et%20al.%20-%20Bioresource%20Technology%20-%20Biokinetics%20of%20microbial%20consortia%20using%20biogenic%20S0.pdf"}, {"href": "https://doi.org/10.1016/j.biortech.2018.09.044"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioresource%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.biortech.2018.09.044", "name": "item", "description": "10.1016/j.biortech.2018.09.044", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.biortech.2018.09.044"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-12-01T00:00:00Z"}}, {"id": "10.1016/j.scitotenv.2025.178872", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-29T16:16:55Z", "type": "Journal Article", "created": "2025-02-18", "title": "The relevance of biochar and co-applied SynComs on maize quality and sustainability: Evidence from field experiments", "description": "Adoption of sustainable maize cropping practices is urgently needed. Synthetic microbial communities (SynComs) made of plant growth-promoting microorganisms (PGPMs), coupled with biochar from residual biomass, offer an environmentally compatible alternative to inorganic fertilizers and may improve soil fertility. This article extends in a two-year field trial with preliminary results obtained in previous pot experiments, monitoring plant physiology, soil biology and chemistry, and kernel metabolomics. Here, we report the synergistic effect of the co-application of biochar, SynComs, and arbuscular mycorrhizal fungi on the soil microbiome, maize growth, and kernel metabolomic profile. SynComs application did not affect the diversity and richness of soil microbial communities; therefore, it posed a low risk of long-term effects on soil microbial ecology. With SynComs and biochar co-application to the soil, the physiology of maize plants was characterized by higher chlorophyll content, ear weight, and kernel weight. The combination of SynComs and biochar also affected the kernel metabolome, resulting in enriched health-beneficial and anti-stress metabolites. Since the preliminary evidence on the environmental and economic impact of these new associations was more favorable than that of conventional fertilizers, it seems reasonable that their large-scale implementation can eventually favor the transition to more sustainable agriculture.", "keywords": ["Biochar", "From field to fork", "Sustainable agriculture", "Biochar; From field to fork; Sustainable agriculture; Synthetic microbial consortia", "Synthetic microbial consortia", "630"]}, "links": [{"href": "https://flore.unifi.it/bitstream/2158/1419273/1/Gull%c3%ac%20et%20al.%202025%20STOTEN.pdf"}, {"href": "https://doi.org/10.1016/j.scitotenv.2025.178872"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.scitotenv.2025.178872", "name": "item", "description": "10.1016/j.scitotenv.2025.178872", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.scitotenv.2025.178872"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-03-01T00:00:00Z"}}, {"id": "10.3389/fmicb.2020.01904", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:20:54Z", "type": "Journal Article", "created": "2020-08-05", "title": "Field Exploitation of Multiple Functions of Beneficial Microorganisms for Plant Nutrition and Protection: Real Possibility or Just a Hope?", "description": "Bioproducts, i.e., microbial based pesticides or fertilizers (biopesticides and biofertilizers), should be expected to play an ever-increasing role and application in agricultural practices world-wide in the effort to implement policies concerned with sustainable agriculture. However, several microbial strains have proven the capacity to augment plant productivity by enhancing crop nutrition and functioning as biopesticides, or vice-versa. This multifunctionality is an issue that is still not included as a concept and possibility in any legal provision regarding the placing on the market of bioproducts, and indicates difficulties in clearly classifying the purpose of their suitability. In this review, we overview the current understanding of the mechanisms in plant-microbe interactions underlining the dual function of microbial strains toward plant nutrition and protection. The prospects of market development for multifunctional bioproducts are then considered in view of the current regulatory approach in the European Union, in an effort that wants to stimulate a wider adoption of the new knowledge on the role played by microorganisms in crop production.", "keywords": ["biofertilizers", "0301 basic medicine", "2. Zero hunger", "biopesticides", "0303 health sciences", "multifunctional bioproducts", "endophytes", "15. Life on land", "Microbiology", "biofertilizers", " biopesticides", " microbial consortia", " multifunctional bioproducts", " plant growth-promoting microorganisms", " endophytes", "QR1-502", "12. Responsible consumption", "03 medical and health sciences", "plant growth-promoting microorganisms", "microbial consortia"], "contacts": [{"organization": "Kowalska Jolanta, Tyburski J\u00f3zef, Matysiak Kinga, Tylkowski Bartosz, Malus\u00e1 Eligio,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.3389/fmicb.2020.01904"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3389/fmicb.2020.01904", "name": "item", "description": "10.3389/fmicb.2020.01904", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3389/fmicb.2020.01904"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-08-05T00:00:00Z"}}, {"id": "10.1038/s41467-018-05980-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:17:48Z", "type": "Journal Article", "created": "2018-08-29", "title": "Land use driven change in soil pH affects microbial carbon cycling processes", "description": "Abstract<p>Soil microorganisms act as gatekeepers for soil\uffe2\uff80\uff93atmosphere carbon exchange by balancing the accumulation and release of soil organic matter. However, poor understanding of the mechanisms responsible hinders the development of effective land management strategies to enhance soil carbon storage. Here we empirically test the link between microbial ecophysiological traits and topsoil carbon content across geographically distributed soils and land use contrasts. We discovered distinct pH controls on microbial mechanisms of carbon accumulation. Land use intensification in low-pH soils that increased the pH above a threshold (~6.2) leads to carbon loss through increased decomposition, following alleviation of acid retardation of microbial growth. However, loss of carbon with intensification in near-neutral pH soils was linked to decreased microbial biomass and reduced growth efficiency that was, in turn, related to trade-offs with stress alleviation and resource acquisition. Thus, less-intensive management practices in near-neutral pH soils have more potential for carbon storage through increased microbial growth efficiency, whereas in acidic soils, microbial growth is a bigger constraint on decomposition rates.</p", "keywords": ["572 Biochemistry", "BACTERIAL", "ILLUMINA SEQUENCING PLATFORM", "550", "Supplementary Data", "QH301 Biology", "General Physics and Astronomy", "microbial ecology", "Soil", "Biomass", "Soil Microbiology", "SDG 15 - Life on Land", "FUNGAL", "2. Zero hunger", "Carbon Isotopes", "Environmental microbiology", "Ecology", "Q", "ecosystem ecology", "Agriculture", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "Grassland", "soil microbiology", "6. Clean water", "COMMUNITY", "GROWTH", "TURNOVER", "570", "PIPELINE", "Science", "Culture and Communities", "General Biochemistry", "Genetics and Molecular Biology", "Microbial Consortia", "General Biochemistry", " Genetics and Molecular Biology", "Article", "Applied microbiology", "QH301", "carbon cycle", "USE EFFICIENCY", "PHYSIOLOGY", "QD415-436 Biochemistry", "Natural Environment Research Council (NERC)", "NE/M017125/1", "General Chemistry", "Carbon Dioxide", "15. Life on land", "Carbon", "United Kingdom", "CLIMATE", "13. Climate action", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://centaur.reading.ac.uk/78980/8/s41467-018-05980-1.pdf"}, {"href": "https://doi.org/10.1038/s41467-018-05980-1"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-018-05980-1", "name": "item", "description": "10.1038/s41467-018-05980-1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-018-05980-1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-09-04T00:00:00Z"}}, {"id": "10.3390/microorganisms9020426", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:12Z", "type": "Journal Article", "created": "2021-02-19", "title": "Identification of Beneficial Microbial Consortia and Bioactive Compounds with Potential as Plant Biostimulants for a Sustainable Agriculture", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>A growing body of evidence demonstrates the potential of various microbes to enhance plant productivity in cropping systems although their successful field application may be impaired by several biotic and abiotic constraints. In the present work, we aimed at developing multifunctional synthetic microbial consortia to be used in combination with suitable bioactive compounds for improving crop yield and quality. Plant growth-promoting microorganisms (PGPMs) with different functional attributes were identified by a bottom-up approach. A comprehensive literature survey on PGPMs associated with maize, wheat, potato and tomato, and on commercial formulations, was conducted by examining peer-reviewed scientific publications and results from relevant European projects. Metagenome fragment recruitments on genomes of potential PGPMs represented in databases were also performed to help identify plant growth-promoting (PGP) strains. Following evidence of their ability to coexist, isolated PGPMs were synthetically assembled into three different microbial consortia. Additionally, the effects of bioactive compounds on the growth of individually PGPMs were tested in starvation conditions. The different combination products based on microbial and non-microbial biostimulants (BS) appear worth considering for greenhouse and open field trials to select those potentially adoptable in sustainable agriculture.</p></article>", "keywords": ["0301 basic medicine", "QH301-705.5", "delivery method", "Plant growth-promoting microorganisms", "SIMBA; sustainable agriculture; plant growth-promoting microorganisms; microbial consortia; metagenome fragment recruitments; delivery methods; in vitro compatibility; bioactive compounds", "630", "Bioactive compounds", "Article", "660.6", "12. Responsible consumption", "03 medical and health sciences", "plant growth-promoting microorganisms", "Delivery methods", "microbial consortia", "plant growth-promoting microorganism", "Biology (General)", "Metagenome fragment recruitments", "bioactive compound", "2. Zero hunger", "0303 health sciences", "bioactive compounds", "660", "in vitro compatibility", "Sustainable agriculture", "metagenome fragment recruitment", "ta4111", "SIMBA", "3. Good health", "sustainable agriculture", "Microbial consortia", "metagenome fragment recruitments", "delivery methods", "In vitro compatibility"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/9/2/426/pdf"}, {"href": "https://www.mdpi.com/2076-2607/9/2/426/pdf"}, {"href": "https://doi.org/10.3390/microorganisms9020426"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms9020426", "name": "item", "description": "10.3390/microorganisms9020426", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms9020426"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-19T00:00:00Z"}}, {"id": "10.1093/femsec/fiv066", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:18:27Z", "type": "Journal Article", "created": "2015-06-20", "title": "Effects Of Warming And Drought On Potential N2o Emissions And Denitrifying Bacteria Abundance In Grasslands With Different Land-Use", "description": "Increased warming in spring and prolonged summer drought may alter soil microbial denitrification. We measured potential denitrification activity and denitrifier marker gene abundances (nirK, nirS, nosZ) in grasslands soils in three geographic regions characterized by site-specific land-use indices (LUI) after warming in spring, at an intermediate sampling and after summer drought. Potential denitrification was significantly increased by warming, but did not persist over the intermediate sampling. At the intermediate sampling, the relevance of grassland land-use intensity was reflected by increased potential N2O production at sites with higher LUI. Abundances of total bacteria did not respond to experimental warming or drought treatments, displaying resilience to minor and short-term effects of climate change. In contrast, nirS- and nirK-type denitrifiers were more influenced by drought in combination with LUI and pH, while the nosZ abundance responded to the summer drought manipulation. Land-use was a strong driver for potential denitrification as grasslands with higher LUI also had greater potentials for N2O emissions. We conclude that both warming and drought affected the denitrifying communities and the potential denitrification in grassland soils. However, these effects are overruled by regional and site-specific differences in soil chemical and physical properties which are also related to grassland land-use intensity.", "keywords": ["0301 basic medicine", "570", "UFSP13-8 Global Change and Biodiversity", "Climate Change", "Microbial Consortia", "580 Plants (Botany)", "Nitric Oxide", "142-005 142-005", "Soil", "03 medical and health sciences", "potential N2O emissions", "RNA", " Ribosomal", " 16S", "2402 Applied Microbiology and Biotechnology", "use index", "Soil Microbiology", "2. Zero hunger", "Biodiversity Exploratories", "denitrification", "Bacteria", "2404 Microbiology", "04 agricultural and veterinary sciences", "15. Life on land", "Grassland", "6. Clean water", "Droughts", "land", "climate change", "Genes", " Bacterial", "13. Climate action", "8. Economic growth", "Denitrification", "0401 agriculture", " forestry", " and fisheries", "grassland", "microbial community", "2303 Ecology"]}, "links": [{"href": "https://doi.org/10.1093/femsec/fiv066"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/FEMS%20Microbiology%20Ecology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/femsec/fiv066", "name": "item", "description": "10.1093/femsec/fiv066", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/femsec/fiv066"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2015-06-19T00:00:00Z"}}, {"id": "10.1111/1758-2229.12049", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:18:44Z", "type": "Journal Article", "created": "2013-03-14", "title": "Soil Phosphorus Depletion And Shifts In Plant Communities Change Bacterial Community Structure In A Long-Term Grassland Management Trial", "description": "Summary<p>Agricultural systems rely on healthy soils and their sustainability requires understanding the long\uffe2\uff80\uff90term impacts of agricultural practices on soils, including microbial communities. We examined the impact of 17 years of land management on soil bacterial communities in a New Zealand randomized\uffe2\uff80\uff90block pasture trial. Significant variation in bacterial community structure related to mowing and plant biomass removal, while nitrogen fertilizer had no effect. Changes in soil chemistry and legume abundance described 52% of the observed variation in the bacterial community structure. Legumes (Trifolium species) were absent in unmanaged plots but increased in abundance with management intensity; 11% of the variation in soil bacterial community structure was attributed to this shift in the plant community. Olsen P explained 10% of the observed heterogeneity, which is likely due to persistent biomass removal resulting in P limitation; Olsen P was significantly lower in plots with biomass removed (14\uffe2\uff80\uff89mg kg\uffe2\uff88\uff921\uffe2\uff80\uff89\uffc2\uffb1\uffe2\uff80\uff891.3SE) compared with plots that were not mown, or where biomass was left after mowing (32\uffe2\uff80\uff89mg kg\uffe2\uff88\uff921\uffe2\uff80\uff89\uffc2\uffb1\uffe2\uff80\uff891.6SE). Our results suggest that removal of plant biomass and associated phosphorus, as well as shifts in the plant community, have greater long\uffe2\uff80\uff90term impacts on soil bacterial community structure than application of nitrogen fertilizers.</p>", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Bacteria", "Nitrogen", "Microbial Consortia", "Population Dynamics", "Agriculture", "Fabaceae", "Phosphorus", "15. Life on land", "Poaceae", "Soil", "03 medical and health sciences", "Biomass", "Fertilizers", "Ecosystem", "Soil Microbiology", "New Zealand"]}, "links": [{"href": "https://doi.org/10.1111/1758-2229.12049"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1758-2229.12049", "name": "item", "description": "10.1111/1758-2229.12049", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1758-2229.12049"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-04-10T00:00:00Z"}}, {"id": "10.1126/science.aap9516", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:19:19Z", "type": "Journal Article", "created": "2018-01-18", "title": "A global atlas of the dominant bacteria found in soil", "description": "A global map of soil bacteria           <p>             Soil bacteria play key roles in regulating terrestrial carbon dynamics, nutrient cycles, and plant productivity. However, the natural histories and distributions of these organisms remain largely undocumented. Delgado-Baquerizo             et al.             provide a survey of the dominant bacterial taxa found around the world. In soil collections from six continents, they found that only 2% of bacterial taxa account for nearly half of the soil bacterial communities across the globe. These dominant taxa could be clustered into ecological groups of co-occurring bacteria that share habitat preferences. The findings will allow for a more predictive understanding of soil bacterial diversity and distribution.           </p>           <p>             Science             , this issue p.             320           </p", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Bacteria", "Microbial Consortia", "15. Life on land", "A global atlas of the dominant bacteria found in soil.", "soil microbial ecology", "03 medical and health sciences", "Atlases as Topic", "13. Climate action", "XXXXXX - Unknown", "bacteria", "soils", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1126/science.aap9516"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1126/science.aap9516", "name": "item", "description": "10.1126/science.aap9516", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1126/science.aap9516"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-01-19T00:00:00Z"}}, {"id": "10.1371/journal.pone.0077212", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:19:38Z", "type": "Journal Article", "created": "2013-10-16", "title": "Carbon-Degrading Enzyme Activities Stimulated By Increased Nutrient Availability In Arctic Tundra Soils", "description": "Climate-induced warming of the Arctic tundra is expected to increase nutrient availability to soil microbes, which in turn may accelerate soil organic matter (SOM) decomposition. We increased nutrient availability via fertilization to investigate the microbial response via soil enzyme activities. Specifically, we measured potential activities of seven enzymes at four temperatures in three soil profiles (organic, organic/mineral interface, and mineral) from untreated native soils and from soils which had been fertilized with nitrogen (N) and phosphorus (P) since 1989 (23 years) and 2006 (six years). Fertilized plots within the 1989 site received annual additions of 10 g N \u00b7 m(-2) \u00b7 year(-1) and 5 g P \u00b7 m(-2) \u00b7 year(-1). Within the 2006 site, two fertilizer regimes were established--one in which plots received 5 g N \u00b7 m(-2) \u00b7 year(-1) and 2.5 g P \u00b7 m(-2) \u00b7 year(-1) and one in which plots received 10 g N \u00b7 m(-2) \u00b7 year(-1) and 5 g P \u00b7 m(-2) \u00b7 year(-1). The fertilization treatments increased activities of enzymes hydrolyzing carbon (C)-rich compounds but decreased phosphatase activities, especially in the organic soils. Activities of two enzymes that degrade N-rich compounds were not affected by the fertilization treatments. The fertilization treatments increased ratios of enzyme activities degrading C-rich compounds to those for N-rich compounds or phosphate, which could lead to changes in SOM chemistry over the long term and to losses of soil C. Accelerated SOM decomposition caused by increased nutrient availability could significantly offset predicted increased C fixation via stimulated net primary productivity in Arctic tundra ecosystems.", "keywords": ["550", "Nitrogen", "Science", "Climate", "Microbial Consortia", "Soil", "soil organic matter", "Fertilizers", "Soil Microbiology", "2. Zero hunger", "decomposition", "Arctic Regions", "Q", "R", "Temperature", "Phosphorus", "04 agricultural and veterinary sciences", "15. Life on land", "Carbon", "Phosphoric Monoester Hydrolases", "soil organic carbon", "13. Climate action", "Medicine", "0401 agriculture", " forestry", " and fisheries", "Arctic tundra ecosystem", "Glucosidases", "Research Article", "Peptide Hydrolases"]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0077212"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0077212", "name": "item", "description": "10.1371/journal.pone.0077212", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0077212"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2013-10-15T00:00:00Z"}}, {"id": "10.1371/journal.pone.0200979", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:19:40Z", "type": "Journal Article", "created": "2019-04-11", "title": "Quantitative and qualitative evaluation of the impact of the G2 enhancer, bead sizes and lysing tubes on the bacterial community composition during DNA extraction from recalcitrant soil core samples based on community sequencing and qPCR", "description": "Abstract<p>Soil DNA extraction encounters numerous challenges that can affect both yield and purity of the recovered DNA. Clay particles lead to reduced DNA extraction efficiency, and PCR inhibitors from the soil matrix can negatively affect downstream analyses when applying DNA sequencing. Further, these effects impede molecular analysis of bacterial community compositions in lower biomass samples, as often observed in deeper soil layers. Many studies avoid these complications by using indirect DNA extraction with prior separation of the cells from the matrix, but such methods introduce other biases that influence the resulting microbial community composition.</p><p>To address these issues, a direct DNA extraction method was applied in combination with the use of a commercial product, the G2 DNA/RNA Enhancer\uffc2\uffae, marketed as being capable of improving the amount of DNA recovered after the lysis step. The results showed that application of G2 increased DNA yields from the studied clayey soils from layers between 1.00 and 2.20 m below ground level.</p><p>Importantly, the use of G2 did not introduce bias, as it did not result in any significant differences in the biodiversity of the bacterial community measured in terms of alpha and beta diversity and taxonomical composition.</p><p>Finally, this study considered a set of customised lysing tubes for evaluating possible influences on the DNA yield. Tubes customization included different bead sizes and amounts, along with lysing tubes coming from two suppliers. Results showed that the lysing tubes with mixed beads allowed greater DNA recovery compared to the use of either 0.1 or 1.4 mm beads, irrespective of the tube supplier.</p><p>These outcomes may help to improve commercial products in DNA/RNA extraction kits, besides raising awareness about the optimal choice of additives, offering opportunities for acquiring a better understanding of topics such as vertical microbial characterisation and environmental DNA recovery in low biomass samples.</p>", "keywords": ["DNA", " Bacterial", "0301 basic medicine", "Science", "Microbial Consortia", "DIVERSITY", "SOFTWARE", "Real-Time Polymerase Chain Reaction", "BACILLUS-SUBTILIS", "BIOMASS", "03 medical and health sciences", "BIOAUGMENTATION", "DNA", " Bacterial/chemistry", "MICROBIAL COMMUNITIES", "Soil Microbiology", "2. Zero hunger", "0303 health sciences", "16S RIBOSOMAL-RNA", "Q", "R", "PROFILES", "ACIDS", "TRANSFORMATION", "6. Clean water", "Microbial Consortia/genetics", "Enhancer Elements", " Genetic", "13. Climate action", "Medicine", "Research Article"]}, "links": [{"href": "https://www.biorxiv.org/content/10.1101/365395v1.full.pdf"}, {"href": "https://doi.org/10.1371/journal.pone.0200979"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLOS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0200979", "name": "item", "description": "10.1371/journal.pone.0200979", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0200979"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-07-09T00:00:00Z"}}, {"id": "10.3390/microorganisms12122562", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:11Z", "type": "Journal Article", "created": "2024-12-12", "title": "Genome Insights into Beneficial Microbial Strains Composing SIMBA Microbial Consortia Applied as Biofertilizers for Maize, Wheat and Tomato", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>For the safe use of microbiome-based solutions in agriculture, the genome sequencing of strains composing the inoculum is mandatory to avoid the spread of virulence and multidrug resistance genes carried by them through horizontal gene transfer to other bacteria in the environment. Moreover, the annotated genomes can enable the design of specific primers to trace the inoculum into the soil and provide insights into the molecular and genetic mechanisms of plant growth promotion and biocontrol activity. In the present work, the genome sequences of some members of beneficial microbial consortia that have previously been tested in greenhouse and field trials as promising biofertilizers for maize, tomato and wheat crops have been determined. Strains belong to well-known plant-growth-promoting bacterial genera such as Bacillus, Burkholderia, Pseudomonas and Rahnella. The genome size of strains ranged from 4.5 to 7.5 Mbp, carrying many genes spanning from 4402 to 6697, and a GC content of 0.04% to 3.3%. The annotation of the genomes revealed the presence of genes that are implicated in functions related to antagonism, pathogenesis and other secondary metabolites possibly involved in plant growth promotion and gene clusters for protection against oxidative damage, confirming the plant-growth-promoting (PGP) activity of selected strains. All the target genomes were found to possess at least 3000 different PGP traits, belonging to the categories of nitrogen acquisition, colonization for plant-derived substrate usage, quorum sensing response for biofilm formation and, to a lesser extent, bacterial fitness and root colonization. No genes putatively involved in pathogenesis were identified. Overall, our study suggests the safe application of selected strains as \u201cplant probiotics\u201d for sustainable agriculture.</p></article>", "keywords": ["biofertilizers", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "traceability", "PGP bacteria", "whole-genome sequencing", "QH301-705.5", "microbial consortia", "risk assessment", "Biology (General)", "Article"], "contacts": [{"organization": "Lisa Cangioli, Silvia Tabacchioni, Andrea Visca, Alessia Fiore, Giuseppe Aprea, Patrizia Ambrosino, Enrico Ercole, Soren S\u00f8rensen, Alessio Mengoni, Annamaria Bevivino,", "roles": ["creator"]}]}, "links": [{"href": "https://iris.enea.it/bitstream/20.500.12079/80427/1/Genome%20Insights%20into%20Beneficial%20Microbial%20Strains%20Composing%20SIMBA%20Microbial%20Consortia%20Applied%20as%20Biofertilizers%20for%20Maize%2c%20Wheat%20and%20Tomato.pdf"}, {"href": "https://doi.org/10.3390/microorganisms12122562"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms12122562", "name": "item", "description": "10.3390/microorganisms12122562", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms12122562"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-12T00:00:00Z"}}, {"id": "10.3390/microorganisms12051002", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:11Z", "type": "Journal Article", "created": "2024-05-16", "title": "Real-Time PCR (qtPCR) to Discover the Fate of Plant Growth-Promoting Rhizobacteria (PGPR) in Agricultural Soils", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>To optimize the application of plant growth-promoting rhizobacteria (PGPR) in field trials, tracking methods are needed to assess their shelf life and to determine the elements affecting their effectiveness and their interactions with plants and native soil microbiota. This work developed a real-time PCR (qtPCR) method which traces and quantifies bacteria when added as microbial consortia, including five PGPR species: Burkholderia ambifaria, Bacillus amyloliquefaciens, Azotobacter chroococcum, Pseudomonas fluorescens, and Rahnella aquatilis. Through a literature search and in silico sequence analyses, a set of primer pairs which selectively tag three bacterial species (B. ambifaria, B. amyloliquefaciens and R. aquatilis) was retrieved. The primers were used to trace these microbial species in a field trial in which the consortium was tested as a biostimulant on two wheat varieties, in combination with biochar and the mycorrhizal fungus Rhizophagus intraradices. The qtPCR assay demonstrated that the targeted bacteria had colonized and grown into the soil, reaching a maximum of growth between 15 and 20 days after inoculum. The results also showed biochar had a positive effect on PGPR growth. In conclusion, qtPCR was once more an effective method to trace the fate of supplied bacterial species in the consortium when used as a cargo system for their delivery.</p></article>", "keywords": ["sustainable agriculture", "0301 basic medicine", "2. Zero hunger", "biostimulants", "0303 health sciences", "03 medical and health sciences", "microbial consortia (MC)", "real-time PCR (qtPCR)", "traceability", "QH301-705.5", "Biology (General)", "plant growth-promoting rhizobacteria (PGPR)", "Article"]}, "links": [{"href": "https://www.mdpi.com/2076-2607/12/5/1002/pdf"}, {"href": "https://doi.org/10.3390/microorganisms12051002"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/microorganisms12051002", "name": "item", "description": "10.3390/microorganisms12051002", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/microorganisms12051002"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-05-16T00:00:00Z"}}, {"id": "10.3390/agronomy12040899", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:01Z", "type": "Journal Article", "created": "2022-04-08", "title": "Effects of Multi-Species Microbial Inoculants on Early Wheat Growth and Litterbag Microbial Activity", "description": "<p>The use of microbial consortia (MC) with complementing features is considered to be a promising method of sustainable crop intensification, potentially trumping the limited performance of single-strain applications. We assessed the effect of two novel MC on early wheat growth and litterbag microbial activity in heated and unheated soil. Pot experiments were carried out in duplicate in a greenhouse over 63 days using a completely randomized design with six replications. A range of parameters of plant growth and nutrient uptake were regularly assessed and statistically analyzed by ANOVA. The litterbag-NIRS method was used to trace the microbial activity. Averaged over both trials, soil heating resulted in a significant increase in shoot biomass (+53%) and subsequent nitrogen uptake (+307 mg N pot\uffe2\uff88\uff921) but strongly reduced root development (\uffe2\uff88\uff9246%) compared with unheated soil. The application of MC had no effect on wheat growth in the heated soil. By contrast, in the unheated soil, shoot (+12%) and root (+15%) biomass and shoot nitrogen uptake (+11%) were significantly increased after double inoculation with MC compared with autoclaved MC. The litterbag-NIRS method confirmed clear effects of soil heating on microbial activity. Differences between MC application and the control were noted, indicating a buffering effect of MC.</p>", "keywords": ["2. Zero hunger", "Greenhouse", "S", "Litterbag-NIRS method", "microbial consortia inoculants", "plant-microbe interactions", "Agriculture", "04 agricultural and veterinary sciences", "Microbial consortia inoculants", "Plant-growth-promoting microorganisms", "plant-growth-promoting microorganisms; microbial consortia inoculants; microbial fertilizer; plant-microbe interactions; pot experiments; greenhouse; litterbag-NIRS method", "microbial fertilizer", "Pot experiments", "plant-growth-promoting microorganisms", "greenhouse", "0401 agriculture", " forestry", " and fisheries", "pot experiments", "Plant-microbe interactions", "Microbial fertilizer"]}, "links": [{"href": "http://www.mdpi.com/2073-4395/12/4/899/pdf"}, {"href": "https://iris.enea.it/bitstream/20.500.12079/70987/1/Effects%20of%20Multi-Species%20Microbial%20Inoculants%20on%20Early%20Wheat%20Growth%20and%20Litterbag%20Microbial%20Activity.pdf"}, {"href": "https://www.mdpi.com/2073-4395/12/4/899/pdf"}, {"href": "https://doi.org/10.3390/agronomy12040899"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agronomy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/agronomy12040899", "name": "item", "description": "10.3390/agronomy12040899", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/agronomy12040899"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-04-08T00:00:00Z"}}, {"id": "10.3390/bioengineering4020055", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:21:03Z", "type": "Journal Article", "created": "2017-06-12", "title": "Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production", "description": "<p>Sustainable biofuels, biomaterials, and fine chemicals production is a critical matter that research teams around the globe are focusing on nowadays. Polyhydroxyalkanoates represent one of the biomaterials of the future due to their physicochemical properties, biodegradability, and biocompatibility. Designing efficient and economic bioprocesses, combined with the respective social and environmental benefits, has brought together scientists from different backgrounds highlighting the multidisciplinary character of such a venture. In the current review, challenges and opportunities regarding polyhydroxyalkanoate production are presented and discussed, covering key steps of their overall production process by applying pure and mixed culture biotechnology, from raw bioprocess development to downstream processing.</p>", "keywords": ["0106 biological sciences", "0301 basic medicine", "Technology", "Renewable feedstock", "QH301-705.5", "biopolymers", "Review", "7. Clean energy", "01 natural sciences", "12. Responsible consumption", "mixed microbial consortia", "03 medical and health sciences", "Biopolymers", "/dk/atira/pure/sustainabledevelopmentgoals/affordable_and_clean_energy; name=SDG 7 - Affordable and Clean Energy", "renewable feedstock", "Pure cultures", "pure cultures", "enrichment strategy", "Biology (General)", "Synthetic biology", "Polyhydroxyalkanoates", "T", "polyhydroxyalkanoates", "Mixed microbial consortia", "downstream processing", "Downstream processing", "13. Climate action", "Enrichment strategy", "synthetic biology"]}, "links": [{"href": "http://www.mdpi.com/2306-5354/4/2/55/pdf"}, {"href": "https://doi.org/10.3390/bioengineering4020055"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioengineering", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.3390/bioengineering4020055", "name": "item", "description": "10.3390/bioengineering4020055", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.3390/bioengineering4020055"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-06-11T00:00:00Z"}}, {"id": "11381/3018253", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-05-29T16:25:13Z", "type": "Journal Article", "created": "2025-02-18", "title": "The relevance of biochar and co-applied SynComs on maize quality and sustainability: Evidence from field experiments", "description": "Adoption of sustainable maize cropping practices is urgently needed. Synthetic microbial communities (SynComs) made of plant growth-promoting microorganisms (PGPMs), coupled with biochar from residual biomass, offer an environmentally compatible alternative to inorganic fertilizers and may improve soil fertility. This article extends in a two-year field trial with preliminary results obtained in previous pot experiments, monitoring plant physiology, soil biology and chemistry, and kernel metabolomics. Here, we report the synergistic effect of the co-application of biochar, SynComs, and arbuscular mycorrhizal fungi on the soil microbiome, maize growth, and kernel metabolomic profile. SynComs application did not affect the diversity and richness of soil microbial communities; therefore, it posed a low risk of long-term effects on soil microbial ecology. With SynComs and biochar co-application to the soil, the physiology of maize plants was characterized by higher chlorophyll content, ear weight, and kernel weight. The combination of SynComs and biochar also affected the kernel metabolome, resulting in enriched health-beneficial and anti-stress metabolites. Since the preliminary evidence on the environmental and economic impact of these new associations was more favorable than that of conventional fertilizers, it seems reasonable that their large-scale implementation can eventually favor the transition to more sustainable agriculture.", "keywords": ["Biochar", "From field to fork", "Sustainable agriculture", "Biochar; From field to fork; Sustainable agriculture; Synthetic microbial consortia", "Synthetic microbial consortia", "630"]}, "links": [{"href": "https://flore.unifi.it/bitstream/2158/1419273/1/Gull%c3%ac%20et%20al.%202025%20STOTEN.pdf"}, {"href": "https://doi.org/11381/3018253"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11381/3018253", "name": "item", "description": "11381/3018253", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11381/3018253"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2025-03-01T00:00:00Z"}}, {"id": "11381/2888724", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:12Z", "type": "Journal Article", "created": "2021-02-19", "title": "Identification of Beneficial Microbial Consortia and Bioactive Compounds with Potential as Plant Biostimulants for a Sustainable Agriculture", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>A growing body of evidence demonstrates the potential of various microbes to enhance plant productivity in cropping systems although their successful field application may be impaired by several biotic and abiotic constraints. In the present work, we aimed at developing multifunctional synthetic microbial consortia to be used in combination with suitable bioactive compounds for improving crop yield and quality. Plant growth-promoting microorganisms (PGPMs) with different functional attributes were identified by a bottom-up approach. A comprehensive literature survey on PGPMs associated with maize, wheat, potato and tomato, and on commercial formulations, was conducted by examining peer-reviewed scientific publications and results from relevant European projects. Metagenome fragment recruitments on genomes of potential PGPMs represented in databases were also performed to help identify plant growth-promoting (PGP) strains. Following evidence of their ability to coexist, isolated PGPMs were synthetically assembled into three different microbial consortia. Additionally, the effects of bioactive compounds on the growth of individually PGPMs were tested in starvation conditions. The different combination products based on microbial and non-microbial biostimulants (BS) appear worth considering for greenhouse and open field trials to select those potentially adoptable in sustainable agriculture.</p></article>", "keywords": ["0301 basic medicine", "QH301-705.5", "delivery method", "Plant growth-promoting microorganisms", "630", "Bioactive compounds", "Article", "660.6", "12. Responsible consumption", "03 medical and health sciences", "plant growth-promoting microorganisms", "Delivery methods", "microbial consortia", "plant growth-promoting microorganism", "Biology (General)", "Metagenome fragment recruitments", "bioactive compound", "2. Zero hunger", "0303 health sciences", "bioactive compounds", "660", "in vitro compatibility", "Sustainable agriculture", "metagenome fragment recruitment", "SIMBA", "3. Good health", "sustainable agriculture", "Microbial consortia", "metagenome fragment recruitments", "delivery methods", "In vitro compatibility"]}, "links": [{"href": "http://www.mdpi.com/2076-2607/9/2/426/pdf"}, {"href": "https://www.mdpi.com/2076-2607/9/2/426/pdf"}, {"href": "https://doi.org/11381/2888724"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11381/2888724", "name": "item", "description": "11381/2888724", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11381/2888724"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-19T00:00:00Z"}}, {"id": "11381/2969532", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:13Z", "type": "Journal Article", "created": "2024-01-19", "title": "Microbial consortia and biochar as sustainable biofertilisers: Analysis of their impact on wheat growth and production", "description": "The European Union is among the top wheat producers in the world, but its productivity relies on adequate soil fertilisation. Biofertilisers, either alone or in combination with biochar, can be a preferable alternative to chemical fertilisers. However, the addition of biofertilisers, specifically plant growth promoting microbes (PGPM), could modify grain composition, and/or deteriorate the soil composition. In this study, the two wheat cultivars Triticum aestivum (Bramante) and T. durum (Svevo) were cultivated in open fields for two consecutive years in the presence of a commercial PGPM mix supplied alone or in combination with biochar. An in-depth analysis was conducted by collecting physiological and agronomic data throughout the growth period. The effects of PGPM and biochar were investigated in detail; specifically, soil chemistry and rhizosphere microbial composition were characterized, along with the treatment effects on seed storage proteins. The results demonstrated that the addition of commercial microbial consortia and biochar, alone or in combination, did not modify the rhizospheric microbial community; however, it increased grain yield, especially in the cultivar Svevo (increase of 6.8\u00a0%-13.6\u00a0%), even though the factors driving the most variations were associated with both climate and cultivar. The total gluten content of the flours was not affected, whereas the main effect of the treatments was a variation in gliadins and low-molecular-weight-glutenin subunits in both cultivars when treated with PGPM and biochar. This suggested improved grain quality, especially regarding the viscoelastic properties of the dough, when the filling period occurred in a dry climate. The results indicate that the application of biofertilisers and biochar may aid the effective management of sustainable wheat cultivation, to support environmental health without altering the biodiversity of the resident microbiome.", "keywords": ["Soil", "Charcoal", "Microbial Consortia", "Edible Grain", "Biochar Gluten Plant growth promoting microbes Sustainable agriculture Wheat", "630", "Triticum"]}, "links": [{"href": "https://doi.org/11381/2969532"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20of%20The%20Total%20Environment", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "11381/2969532", "name": "item", "description": "11381/2969532", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/11381/2969532"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-03-01T00:00:00Z"}}, {"id": "20.500.12079/65687", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:46Z", "type": "Other", "title": "Identification of beneficial microbial consortia and bioactive compounds with potential as plant biostimulants for a sustainable agriculture", "description": "A growing body of evidence demonstrates the potential of various microbes to enhance plant productivity in cropping systems although their successful field application may be impaired by several biotic and abiotic constraints. In the present work, we aimed at developing multifunctional synthetic microbial consortia to be used in combination with suitable bioactive compounds for improving crop yield and quality. Plant growth-promoting microorganisms (PGPMs) with different functional attributes were identified by a bottom-up approach. A comprehensive literature survey on PGPMs associated with maize, wheat, potato and tomato, and on commercial formulations, was conducted by examining peer-reviewed scientific publications and results from relevant European projects. Metagenome fragment recruitments on genomes of potential PGPMs represented in databases were also performed to help identify plant growth-promoting (PGP) strains. Following evidence of their ability to coexist, isolated PGPMs were synthetically assembled into three different microbial consortia. Additionally, the effects of bioactive compounds on the growth of individually PGPMs were tested in starvation conditions. The different combination products based on microbial and non-microbial biostimulants (BS) appear worth considering for greenhouse and open field trials to select those potentially adoptable in sustainable agriculture.", "keywords": ["Microbial consortia", "Delivery methods", "Sustainable agriculture", "Plant growth-promoting microorganisms", "Metagenome fragment recruitments", "Bioactive compounds", "In vitro compatibility", "SIMBA"], "contacts": [{"organization": "Tabacchioni S., Passato S., Ambrosino P., Huang L., Caldara M., Cantale C., Hett J., Del Fiore A., Fiore A., Schluter A., Sczyrba A., Maestri E., Marmiroli N., Neuhoff D., Nesme J., Sorensen S. J., Aprea G., Nobili C., Presenti O., Giovannetti G., Giovannetti C., Pihlanto A., Brunori A., Bevivino A.,", "roles": ["creator"]}]}, "links": [{"href": "https://iris.enea.it/bitstream/20.500.12079/65687/3/Identification%20of%20Beneficial%20Microbial%20Consortia%20and%20Bioactive%20Compounds%20with%20Potential%20as%20Plant%20Biostimulants%20for%20a%20Sustainable%20Agriculture.pdf"}, {"href": "https://doi.org/20.500.12079/65687"}, {"rel": "self", "type": "application/geo+json", "title": "20.500.12079/65687", "name": "item", "description": "20.500.12079/65687", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/20.500.12079/65687"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "20.500.12079/70987", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:46Z", "type": "Journal Article", "created": "2022-04-08", "title": "Effects of Multi-Species Microbial Inoculants on Early Wheat Growth and Litterbag Microbial Activity", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>The use of microbial consortia (MC) with complementing features is considered to be a promising method of sustainable crop intensification, potentially trumping the limited performance of single-strain applications. We assessed the effect of two novel MC on early wheat growth and litterbag microbial activity in heated and unheated soil. Pot experiments were carried out in duplicate in a greenhouse over 63 days using a completely randomized design with six replications. A range of parameters of plant growth and nutrient uptake were regularly assessed and statistically analyzed by ANOVA. The litterbag-NIRS method was used to trace the microbial activity. Averaged over both trials, soil heating resulted in a significant increase in shoot biomass (+53%) and subsequent nitrogen uptake (+307 mg N pot\u22121) but strongly reduced root development (\u221246%) compared with unheated soil. The application of MC had no effect on wheat growth in the heated soil. By contrast, in the unheated soil, shoot (+12%) and root (+15%) biomass and shoot nitrogen uptake (+11%) were significantly increased after double inoculation with MC compared with autoclaved MC. The litterbag-NIRS method confirmed clear effects of soil heating on microbial activity. Differences between MC application and the control were noted, indicating a buffering effect of MC.</p></article>", "keywords": ["2. Zero hunger", "Greenhouse", "S", "Litterbag-NIRS method", "microbial consortia inoculants", "plant-microbe interactions", "Agriculture", "04 agricultural and veterinary sciences", "Microbial consortia inoculants", "Plant-growth-promoting microorganisms", "plant-growth-promoting microorganisms; microbial consortia inoculants; microbial fertilizer; plant-microbe interactions; pot experiments; greenhouse; litterbag-NIRS method", "microbial fertilizer", "Pot experiments", "plant-growth-promoting microorganisms", "greenhouse", "0401 agriculture", " forestry", " and fisheries", "pot experiments", "Plant-microbe interactions", "Microbial fertilizer"]}, "links": [{"href": "http://www.mdpi.com/2073-4395/12/4/899/pdf"}, {"href": "https://iris.enea.it/bitstream/20.500.12079/70987/1/Effects%20of%20Multi-Species%20Microbial%20Inoculants%20on%20Early%20Wheat%20Growth%20and%20Litterbag%20Microbial%20Activity.pdf"}, {"href": "https://www.mdpi.com/2073-4395/12/4/899/pdf"}, {"href": "https://doi.org/20.500.12079/70987"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Agronomy", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "20.500.12079/70987", "name": "item", "description": "20.500.12079/70987", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/20.500.12079/70987"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2022-04-08T00:00:00Z"}}, {"id": "20.500.12079/80427", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:46Z", "type": "Journal Article", "created": "2024-12-12", "title": "Genome Insights into Beneficial Microbial Strains Composing SIMBA Microbial Consortia Applied as Biofertilizers for Maize, Wheat and Tomato", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>For the safe use of microbiome-based solutions in agriculture, the genome sequencing of strains composing the inoculum is mandatory to avoid the spread of virulence and multidrug resistance genes carried by them through horizontal gene transfer to other bacteria in the environment. Moreover, the annotated genomes can enable the design of specific primers to trace the inoculum into the soil and provide insights into the molecular and genetic mechanisms of plant growth promotion and biocontrol activity. In the present work, the genome sequences of some members of beneficial microbial consortia that have previously been tested in greenhouse and field trials as promising biofertilizers for maize, tomato and wheat crops have been determined. Strains belong to well-known plant-growth-promoting bacterial genera such as Bacillus, Burkholderia, Pseudomonas and Rahnella. The genome size of strains ranged from 4.5 to 7.5 Mbp, carrying many genes spanning from 4402 to 6697, and a GC content of 0.04% to 3.3%. The annotation of the genomes revealed the presence of genes that are implicated in functions related to antagonism, pathogenesis and other secondary metabolites possibly involved in plant growth promotion and gene clusters for protection against oxidative damage, confirming the plant-growth-promoting (PGP) activity of selected strains. All the target genomes were found to possess at least 3000 different PGP traits, belonging to the categories of nitrogen acquisition, colonization for plant-derived substrate usage, quorum sensing response for biofilm formation and, to a lesser extent, bacterial fitness and root colonization. No genes putatively involved in pathogenesis were identified. Overall, our study suggests the safe application of selected strains as \u201cplant probiotics\u201d for sustainable agriculture.</p></article>", "keywords": ["biofertilizers", "0301 basic medicine", "0303 health sciences", "03 medical and health sciences", "traceability", "PGP bacteria", "whole-genome sequencing", "QH301-705.5", "microbial consortia", "risk assessment", "Biology (General)", "Article"]}, "links": [{"href": "https://iris.enea.it/bitstream/20.500.12079/80427/1/Genome%20Insights%20into%20Beneficial%20Microbial%20Strains%20Composing%20SIMBA%20Microbial%20Consortia%20Applied%20as%20Biofertilizers%20for%20Maize%2c%20Wheat%20and%20Tomato.pdf"}, {"href": "https://www.mdpi.com/2076-2607/12/12/2562/pdf"}, {"href": "https://doi.org/20.500.12079/80427"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "20.500.12079/80427", "name": "item", "description": "20.500.12079/80427", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/20.500.12079/80427"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-12-12T00:00:00Z"}}, {"id": "2164/13294", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:25:56Z", "type": "Journal Article", "created": "2018-08-29", "title": "Land use driven change in soil pH affects microbial carbon cycling processes", "description": "Abstract<p>Soil microorganisms act as gatekeepers for soil\uffe2\uff80\uff93atmosphere carbon exchange by balancing the accumulation and release of soil organic matter. However, poor understanding of the mechanisms responsible hinders the development of effective land management strategies to enhance soil carbon storage. Here we empirically test the link between microbial ecophysiological traits and topsoil carbon content across geographically distributed soils and land use contrasts. We discovered distinct pH controls on microbial mechanisms of carbon accumulation. Land use intensification in low-pH soils that increased the pH above a threshold (~6.2) leads to carbon loss through increased decomposition, following alleviation of acid retardation of microbial growth. However, loss of carbon with intensification in near-neutral pH soils was linked to decreased microbial biomass and reduced growth efficiency that was, in turn, related to trade-offs with stress alleviation and resource acquisition. Thus, less-intensive management practices in near-neutral pH soils have more potential for carbon storage through increased microbial growth efficiency, whereas in acidic soils, microbial growth is a bigger constraint on decomposition rates.</p", "keywords": ["572 Biochemistry", "BACTERIAL", "ILLUMINA SEQUENCING PLATFORM", "550", "Supplementary Data", "QH301 Biology", "General Physics and Astronomy", "microbial ecology", "Soil", "Biomass", "Soil Microbiology", "SDG 15 - Life on Land", "FUNGAL", "2. Zero hunger", "Carbon Isotopes", "Environmental microbiology", "Ecology", "Q", "ecosystem ecology", "Agriculture", "04 agricultural and veterinary sciences", "Hydrogen-Ion Concentration", "Grassland", "soil microbiology", "6. Clean water", "COMMUNITY", "GROWTH", "TURNOVER", "570", "PIPELINE", "Science", "Culture and Communities", "General Biochemistry", "Genetics and Molecular Biology", "Microbial Consortia", "General Biochemistry", " Genetics and Molecular Biology", "Article", "Applied microbiology", "QH301", "carbon cycle", "USE EFFICIENCY", "PHYSIOLOGY", "QD415-436 Biochemistry", "Natural Environment Research Council (NERC)", "NE/M017125/1", "General Chemistry", "Carbon Dioxide", "15. Life on land", "Carbon", "United Kingdom", "CLIMATE", "13. Climate action", "0401 agriculture", " forestry", " and fisheries"]}, "links": [{"href": "https://centaur.reading.ac.uk/78980/8/s41467-018-05980-1.pdf"}, {"href": "https://doi.org/2164/13294"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2164/13294", "name": "item", "description": "2164/13294", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2164/13294"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-09-04T00:00:00Z"}}, {"id": "2783192416", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:26:08Z", "type": "Journal Article", "created": "2018-01-18", "title": "A global atlas of the dominant bacteria found in soil", "description": "A global map of soil bacteria           <p>             Soil bacteria play key roles in regulating terrestrial carbon dynamics, nutrient cycles, and plant productivity. However, the natural histories and distributions of these organisms remain largely undocumented. Delgado-Baquerizo             et al.             provide a survey of the dominant bacterial taxa found around the world. In soil collections from six continents, they found that only 2% of bacterial taxa account for nearly half of the soil bacterial communities across the globe. These dominant taxa could be clustered into ecological groups of co-occurring bacteria that share habitat preferences. The findings will allow for a more predictive understanding of soil bacterial diversity and distribution.           </p>           <p>             Science             , this issue p.             320           </p", "keywords": ["0301 basic medicine", "2. Zero hunger", "0303 health sciences", "Bacteria", "Microbial Consortia", "15. Life on land", "A global atlas of the dominant bacteria found in soil.", "soil microbial ecology", "03 medical and health sciences", "Atlases as Topic", "13. Climate action", "XXXXXX - Unknown", "bacteria", "soils", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/2783192416"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "2783192416", "name": "item", "description": "2783192416", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/2783192416"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-01-19T00:00:00Z"}}, {"id": "29348236", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:26:14Z", "type": "Journal Article", "created": "2018-01-18", "title": "A global atlas of the dominant bacteria found in soil", "description": "A global map of soil bacteria                   <p>                     Soil bacteria play key roles in regulating terrestrial carbon dynamics, nutrient cycles, and plant productivity. However, the natural histories and distributions of these organisms remain largely undocumented. Delgado-Baquerizo                     et al.                     provide a survey of the dominant bacterial taxa found around the world. In soil collections from six continents, they found that only 2% of bacterial taxa account for nearly half of the soil bacterial communities across the globe. These dominant taxa could be clustered into ecological groups of co-occurring bacteria that share habitat preferences. The findings will allow for a more predictive understanding of soil bacterial diversity and distribution.                   </p>                   <p>                     Science                     , this issue p.                     320                   </p", "keywords": ["2. Zero hunger", "0301 basic medicine", "0303 health sciences", "Bacteria", "Microbial Consortia", "15. Life on land", "soil microbial ecology", "03 medical and health sciences", "Atlases as Topic", "13. Climate action", "XXXXXX - Unknown", "bacteria", "soils", "Phylogeny", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/29348236"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "29348236", "name": "item", "description": "29348236", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/29348236"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-01-19T00:00:00Z"}}, {"id": "3047019976", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:26:25Z", "type": "Journal Article", "created": "2020-08-05", "title": "Field Exploitation of Multiple Functions of Beneficial Microorganisms for Plant Nutrition and Protection: Real Possibility or Just a Hope?", "description": "Bioproducts, i.e., microbial based pesticides or fertilizers (biopesticides and biofertilizers), should be expected to play an ever-increasing role and application in agricultural practices world-wide in the effort to implement policies concerned with sustainable agriculture. However, several microbial strains have proven the capacity to augment plant productivity by enhancing crop nutrition and functioning as biopesticides, or vice-versa. This multifunctionality is an issue that is still not included as a concept and possibility in any legal provision regarding the placing on the market of bioproducts, and indicates difficulties in clearly classifying the purpose of their suitability. In this review, we overview the current understanding of the mechanisms in plant-microbe interactions underlining the dual function of microbial strains toward plant nutrition and protection. The prospects of market development for multifunctional bioproducts are then considered in view of the current regulatory approach in the European Union, in an effort that wants to stimulate a wider adoption of the new knowledge on the role played by microorganisms in crop production.", "keywords": ["biofertilizers", "0301 basic medicine", "2. Zero hunger", "biopesticides", "0303 health sciences", "multifunctional bioproducts", "endophytes", "15. Life on land", "Microbiology", "biofertilizers", " biopesticides", " microbial consortia", " multifunctional bioproducts", " plant growth-promoting microorganisms", " endophytes", "QR1-502", "12. Responsible consumption", "03 medical and health sciences", "plant growth-promoting microorganisms", "microbial consortia"]}, "links": [{"href": "https://doi.org/3047019976"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "3047019976", "name": "item", "description": "3047019976", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/3047019976"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-08-05T00:00:00Z"}}, {"id": "PMC7419637", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:28:44Z", "type": "Journal Article", "created": "2020-08-05", "title": "Field Exploitation of Multiple Functions of Beneficial Microorganisms for Plant Nutrition and Protection: Real Possibility or Just a Hope?", "description": "Bioproducts, i.e., microbial based pesticides or fertilizers (biopesticides and biofertilizers), should be expected to play an ever-increasing role and application in agricultural practices world-wide in the effort to implement policies concerned with sustainable agriculture. However, several microbial strains have proven the capacity to augment plant productivity by enhancing crop nutrition and functioning as biopesticides, or vice-versa. This multifunctionality is an issue that is still not included as a concept and possibility in any legal provision regarding the placing on the market of bioproducts, and indicates difficulties in clearly classifying the purpose of their suitability. In this review, we overview the current understanding of the mechanisms in plant-microbe interactions underlining the dual function of microbial strains toward plant nutrition and protection. The prospects of market development for multifunctional bioproducts are then considered in view of the current regulatory approach in the European Union, in an effort that wants to stimulate a wider adoption of the new knowledge on the role played by microorganisms in crop production.", "keywords": ["biofertilizers", "0301 basic medicine", "2. Zero hunger", "biopesticides", "0303 health sciences", "multifunctional bioproducts", "endophytes", "15. Life on land", "Microbiology", "QR1-502", "12. Responsible consumption", "03 medical and health sciences", "plant growth-promoting microorganisms", "microbial consortia"]}, "links": [{"href": "https://doi.org/PMC7419637"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Frontiers%20in%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC7419637", "name": "item", "description": "PMC7419637", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC7419637"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-08-05T00:00:00Z"}}, {"id": "PMC11124357", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:28:39Z", "type": "Journal Article", "created": "2024-05-16", "title": "Real-Time PCR (qtPCR) to Discover the Fate of Plant Growth-Promoting Rhizobacteria (PGPR) in Agricultural Soils", "description": "<?xml version='1.0' encoding='UTF-8'?><article><p>To optimize the application of plant growth-promoting rhizobacteria (PGPR) in field trials, tracking methods are needed to assess their shelf life and to determine the elements affecting their effectiveness and their interactions with plants and native soil microbiota. This work developed a real-time PCR (qtPCR) method which traces and quantifies bacteria when added as microbial consortia, including five PGPR species: Burkholderia ambifaria, Bacillus amyloliquefaciens, Azotobacter chroococcum, Pseudomonas fluorescens, and Rahnella aquatilis. Through a literature search and in silico sequence analyses, a set of primer pairs which selectively tag three bacterial species (B. ambifaria, B. amyloliquefaciens and R. aquatilis) was retrieved. The primers were used to trace these microbial species in a field trial in which the consortium was tested as a biostimulant on two wheat varieties, in combination with biochar and the mycorrhizal fungus Rhizophagus intraradices. The qtPCR assay demonstrated that the targeted bacteria had colonized and grown into the soil, reaching a maximum of growth between 15 and 20 days after inoculum. The results also showed biochar had a positive effect on PGPR growth. In conclusion, qtPCR was once more an effective method to trace the fate of supplied bacterial species in the consortium when used as a cargo system for their delivery.</p></article>", "keywords": ["sustainable agriculture", "0301 basic medicine", "2. Zero hunger", "biostimulants", "0303 health sciences", "03 medical and health sciences", "microbial consortia (MC)", "real-time PCR (qtPCR)", "traceability", "QH301-705.5", "Biology (General)", "plant growth-promoting rhizobacteria (PGPR)", "Article"]}, "links": [{"href": "https://www.mdpi.com/2076-2607/12/5/1002/pdf"}, {"href": "https://doi.org/PMC11124357"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "PMC11124357", "name": "item", "description": "PMC11124357", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PMC11124357"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-05-16T00:00:00Z"}}, {"id": "f4d16216679d8302f3d39a54f3fd8024", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:31:00Z", "type": "Journal Article", "title": "Identification of Beneficial Microbial Consortia and Bioactive Compounds with Potential as Plant Biostimulants for a Sustainable Agriculture", "keywords": ["sustainable agriculture", "bioactive compounds", "plant growth-promoting microorganisms", "metagenome fragment recruitments", "in vitro compatibility", "microbial consortia", "ta4111", "delivery methods", "SIMBA"], "contacts": [{"organization": "Tabacchioni, Silvia, Passato, Stefania, Ambrosino, Patrizia, Huang, Liren, Caldara, Marina, Cantale, Cristina, Hett, Jonas, Del Fiore, Antonella, Fiore, Alessia, Schl\u00fcter, Andreas, Sczyrba, Alexander, Maestri, Elena, Marmiroli, Nelson, Neuhoff, Daniel, Nesme, Joseph, S\u00f8rensen, S\u00f8ren Johannes, Aprea, Giuseppe, Nobili, Chiara, Presenti, Ombretta, Giovannetti, Giusto, Giovannetti, Caterina, Pihlanto, Anne, Brunori, Andrea, Bevivino, Annamaria,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/f4d16216679d8302f3d39a54f3fd8024"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Microorganisms", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "f4d16216679d8302f3d39a54f3fd8024", "name": "item", "description": "f4d16216679d8302f3d39a54f3fd8024", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/f4d16216679d8302f3d39a54f3fd8024"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}, {"id": "oai:iris.enea.it:20.500.12079/65687", "type": "Feature", "geometry": null, "properties": {"updated": "2026-05-29T16:33:54Z", "type": "Other", "title": "Identification of beneficial microbial consortia and bioactive compounds with potential as plant biostimulants for a sustainable agriculture", "description": "A growing body of evidence demonstrates the potential of various microbes to enhance plant productivity in cropping systems although their successful field application may be impaired by several biotic and abiotic constraints. In the present work, we aimed at developing multifunctional synthetic microbial consortia to be used in combination with suitable bioactive compounds for improving crop yield and quality. Plant growth-promoting microorganisms (PGPMs) with different functional attributes were identified by a bottom-up approach. A comprehensive literature survey on PGPMs associated with maize, wheat, potato and tomato, and on commercial formulations, was conducted by examining peer-reviewed scientific publications and results from relevant European projects. Metagenome fragment recruitments on genomes of potential PGPMs represented in databases were also performed to help identify plant growth-promoting (PGP) strains. Following evidence of their ability to coexist, isolated PGPMs were synthetically assembled into three different microbial consortia. Additionally, the effects of bioactive compounds on the growth of individually PGPMs were tested in starvation conditions. The different combination products based on microbial and non-microbial biostimulants (BS) appear worth considering for greenhouse and open field trials to select those potentially adoptable in sustainable agriculture.", "keywords": ["Microbial consortia", "Delivery methods", "Sustainable agriculture", "Plant growth-promoting microorganisms", "Metagenome fragment recruitments", "Bioactive compounds", "In vitro compatibility", "SIMBA"], "contacts": [{"organization": "Tabacchioni S., Passato S., Ambrosino P., Huang L., Caldara M., Cantale C., Hett J., Del Fiore A., Fiore A., Schluter A., Sczyrba A., Maestri E., Marmiroli N., Neuhoff D., Nesme J., Sorensen S. J., Aprea G., Nobili C., Presenti O., Giovannetti G., Giovannetti C., Pihlanto A., Brunori A., Bevivino A.,", "roles": ["creator"]}]}, "links": [{"href": "https://iris.enea.it/bitstream/20.500.12079/65687/3/Identification%20of%20Beneficial%20Microbial%20Consortia%20and%20Bioactive%20Compounds%20with%20Potential%20as%20Plant%20Biostimulants%20for%20a%20Sustainable%20Agriculture.pdf"}, {"href": "https://doi.org/oai:iris.enea.it:20.500.12079/65687"}, {"rel": "self", "type": "application/geo+json", "title": "oai:iris.enea.it:20.500.12079/65687", "name": "item", "description": "oai:iris.enea.it:20.500.12079/65687", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/oai:iris.enea.it:20.500.12079/65687"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-01-01T00:00:00Z"}}], "links": [{"rel": "self", "type": "application/geo+json", "title": "This document as GeoJSON", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Microbial+Consortia&f=json", "hreflang": "en-US"}, {"rel": "alternate", "type": "text/html", "title": "This document as HTML", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Microbial+Consortia&f=html", "hreflang": "en-US"}, {"rel": "collection", "type": "application/json", "title": "Collection URL", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main", "hreflang": "en-US"}, {"type": "application/geo+json", "rel": "first", "title": "items (first)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Microbial+Consortia&", "hreflang": "en-US"}, {"rel": "last", "type": "application/geo+json", "title": "items (last)", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items?keywords=Microbial+Consortia&offset=29", "hreflang": "en-US"}], "numberMatched": 29, "numberReturned": 29, "distributedFeatures": [], "timeStamp": "2026-05-30T11:08:43.970885Z"}