{"type": "FeatureCollection", "features": [{"id": "10.1016/j.jafr.2023.100732", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:16:52Z", "type": "Journal Article", "created": "2023-08-07", "title": "Aeromycological studies in the crops of the main cereals: A systematic review", "description": "Open AccessLes \u00e9tudes a\u00e9romycologiques sur les cultures c\u00e9r\u00e9ali\u00e8res permettent de d\u00e9terminer la variation temporelle des agents pathog\u00e8nes des plantes affectant la culture et de d\u00e9terminer le moment appropri\u00e9 pour appliquer les fongicides. Cependant, ce sujet n'a pas \u00e9t\u00e9 syst\u00e9matiquement revu. L'objectif de ce travail \u00e9tait d'analyser syst\u00e9matiquement toutes les \u00e9tudes a\u00e9romycologiques r\u00e9alis\u00e9es sur le ma\u00efs, le bl\u00e9, le riz, l'avoine, l'orge, le seigle, le sorgho et le millet. Une recherche syst\u00e9matique a \u00e9t\u00e9 effectu\u00e9e dans Scopus depuis le d\u00e9but de la base de donn\u00e9es jusqu'au 1er ao\u00fbt 2022. Les crit\u00e8res d'inclusion \u00e9taient qu'il s'agissait d'\u00e9tudes a\u00e9romycologiques sur le bl\u00e9 ou le riz ou le ma\u00efs ou l'avoine ou le sorgho ou le seigle ou l'orge ou le millet et d'\u00e9tudes publi\u00e9es dans des revues \u00e0 comit\u00e9 de lecture index\u00e9es dans Journal Citation Reports et r\u00e9dig\u00e9es en anglais ou en espagnol. Quarante-trois \u00e9tudes (21 sur le bl\u00e9, 15 sur le riz, 5 sur le ma\u00efs, 1 sur le sorgho et 2 sur l'orge) r\u00e9pondant \u00e0 tous les crit\u00e8res d'\u00e9ligibilit\u00e9 ont \u00e9t\u00e9 incluses (une des \u00e9tudes sur le ma\u00efs a \u00e9galement \u00e9t\u00e9 men\u00e9e sur le bl\u00e9). Aucune \u00e9tude a\u00e9romycologique n'a \u00e9t\u00e9 trouv\u00e9e chez l'avoine, le seigle et le millet. Il a \u00e9t\u00e9 not\u00e9 que la plupart des recherches a\u00e9romycologiques ont \u00e9t\u00e9 men\u00e9es sur les cultures de bl\u00e9 et principalement dans les pays des Am\u00e9riques. De plus, les propagules fongiques sont principalement collect\u00e9es par des m\u00e9thodes non viables, en utilisant divers types de collecteurs. En g\u00e9n\u00e9ral, les \u00e9tudes visaient \u00e0 identifier un agent pathog\u00e8ne sp\u00e9cifique et non \u00e0 la diversit\u00e9 des agents pathog\u00e8nes qui peuvent \u00eatre trouv\u00e9s. La relation des champignons identifi\u00e9s avec les param\u00e8tres m\u00e9t\u00e9orologiques \u00e9tait variable dans les diff\u00e9rentes \u00e9tudes. Cette revue syst\u00e9matique permet de r\u00e9sumer les \u00e9tudes a\u00e9romycologiques qui ont \u00e9t\u00e9 men\u00e9es sur les cultures de bl\u00e9, de riz, de ma\u00efs, de sorgho et d'orge. Il sugg\u00e8re \u00e9galement o\u00f9 les futures \u00e9tudes dans ce domaine devraient \u00eatre dirig\u00e9es, en fonction des limites rencontr\u00e9es.", "keywords": ["Impacts of Elevated CO2 and Ozone on Plant Physiology", "Agriculture (General)", "Health", " Toxicology and Mutagenesis", "Plant Science", "Crop", "S1-972", "Agricultural and Biological Sciences", "Barley", "Biochemistry", " Genetics and Molecular Biology", "TX341-641", "10. No inequality", "Biology", "Sorghum", "2. Zero hunger", "Corn", "Airborne spores", "Nutrition. Foods and food supply", "Life Sciences", "Phylogenetic Analysis", "Cell Biology", "15. Life on land", "2414.06 Hongos", "Agronomy", "3. Good health", "Wheat", "Environmental Science", "Physical Sciences", "Rice", "Indoor Air Quality and Health Effects", "Diversity and Evolution of Fungal Pathogens", "Biotechnology"]}, "links": [{"href": "https://doi.org/10.1016/j.jafr.2023.100732"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Journal%20of%20Agriculture%20and%20Food%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.jafr.2023.100732", "name": "item", "description": "10.1016/j.jafr.2023.100732", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.jafr.2023.100732"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-01T00:00:00Z"}}, {"id": "10.1016/j.cell.2021.04.024", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:16:08Z", "type": "Journal Article", "created": "2021-05-18", "title": "Innovation, conservation, and repurposing of gene function in root cell type development", "description": "Plant species have evolved myriads of solutions, including complex cell type development and regulation, to adapt to dynamic environments. To understand this cellular diversity, we profiled tomato root cell type translatomes. Using xylem differentiation in tomato, examples of functional innovation, repurposing, and conservation of transcription factors are described, relative to the model plant Arabidopsis. Repurposing and innovation of genes are further observed within an exodermis regulatory network and illustrate its function. Comparative translatome analyses of rice, tomato, and Arabidopsis cell populations suggest increased expression conservation of root meristems compared with other homologous populations. In addition, the functions of constitutively expressed genes are more conserved than those of cell type/tissue-enriched genes. These observations suggest that higher order properties of cell type and pan-cell type regulation are evolutionarily conserved between plants and animals.", "keywords": ["root development", "translatomes", "General Biochemistry", "Genetics and Molecular Biology", "Green Fluorescent Proteins", "Meristem", "Arabidopsis", "cell types; evolution; exodermis; gene regulation; rice; root development; tomato; translatomes; xylem", "tomato", "xylem", "Genes", " Plant", "Plant Roots", "Inventions", "Solanum lycopersicum", "Species Specificity", "Gene Expression Regulation", " Plant", "Xylem", "evolution", "Gene Regulatory Networks", "Promoter Regions", " Genetic", "Plant Proteins", "2. Zero hunger", "exodermis", "rice", "15. Life on land", "Protein Biosynthesis", "cell types", "gene regulation", "Transcription Factors"]}, "links": [{"href": "https://www.research.unipd.it/bitstream/11577/3392826/2/PIIS0092867421005043.pdf"}, {"href": "https://doi.org/10.1016/j.cell.2021.04.024"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Cell", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.cell.2021.04.024", "name": "item", "description": "10.1016/j.cell.2021.04.024", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.cell.2021.04.024"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-06-01T00:00:00Z"}}, {"id": "10.1016/j.coisb.2021.100379", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:16:11Z", "type": "Journal Article", "created": "2021-09-09", "title": "Approaches for completing metabolic networks through metabolite damage and repair discovery", "description": "Metabolites are prone to damage, either via enzymatic side reactions, which collectively form the underground metabolism, or via spontaneous chemical reactions. The resulting non-canonical metabolites that can be toxic, are mended by dedicated 'metabolite repair enzymes.' Deficiencies in the latter can cause severe disease in humans, whereas inclusion of repair enzymes in metabolically engineered systems can improve the production yield of value-added chemicals. The metabolite damage and repair loops are typically not yet included in metabolic reconstructions and it is likely that many remain to be discovered. Here, we review strategies and associated challenges for unveiling non-canonical metabolites and metabolite repair enzymes, including systematic approaches based on high-resolution mass spectrometry, metabolome-wide side-activity prediction, as well as high-throughput substrate and phenotypic screens.", "keywords": ["0301 basic medicine", "0303 health sciences", "03 medical and health sciences", ": Biochemistry", " biophysics & molecular biology [F05] [Life sciences]", ": Biochimie", " biophysique & biologie mol\u00e9culaire [F05] [Sciences du vivant]", "Article"]}, "links": [{"href": "https://doi.org/10.1016/j.coisb.2021.100379"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Current%20Opinion%20in%20Systems%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.coisb.2021.100379", "name": "item", "description": "10.1016/j.coisb.2021.100379", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.coisb.2021.100379"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-12-01T00:00:00Z"}}, {"id": "10.1016/j.cub.2020.09.063", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:16:13Z", "type": "Journal Article", "created": "2020-10-15", "title": "Newly explored\u00a0Faecalibacterium\u00a0diversity is connected to age, lifestyle, geography, and disease.", "description": "Faecalibacterium is prevalent in the human gut and a promising microbe for the development of next-generation probiotics (NGPs) or biotherapeutics. Analyzing reference Faecalibacterium genomes and almost 3,000 Faecalibacterium-like metagenome-assembled genomes (MAGs) reconstructed from 7,907 human and 203 non-human primate gut metagenomes, we identified the presence of 22 different Faecalibacterium-like species-level genome bins (SGBs), some further divided in different strains according to the subject geographical origin. Twelve SGBs are globally spread in the human gut and show different genomic potential in the utilization of complex polysaccharides, suggesting that higher SGB diversity may be related with increased utilization of plant-based foods. Moreover, up to 11 different species may co-occur in the same subject, with lower diversity in Western populations, as well as intestinal inflammatory states and obesity. The newly explored Faecalibacterium diversity will be able to support the choice of strains suitable as NGPs, guided by the consideration of the differences existing in their functional potential.", "keywords": ["Adult", "0301 basic medicine", "pangenome", "Adolescent", "gut microbiome", "Datasets as Topic", "General Biochemistry", " Genetics and Molecular Biology", "Innovation action", "Feces", "03 medical and health sciences", "Animals", "Humans", "biotherapeutics", "European Commission", "Child", "Life Style", "Faecalibacterium", "Aged", "Aurora Universities Network", "Horizon 2020", "0303 health sciences", "EC", "Geography", "Faecalibacterium prausnitzii", "H2020", "Age Factors", "Infant", "Middle Aged", "Gastrointestinal Microbiome", "Faecalibacterium prausnitzii", " gut microbiome", " strain diversity", " pangenome", " novel probiotics", " biotherapeutics", "Child", " Preschool", "novel probiotics", "Dysbiosis", "Macaca", "Metagenome", "strain diversity", "Metagenomics", "General Agricultural and Biological Sciences"]}, "links": [{"href": "https://www.iris.unina.it/bitstream/11588/819607/1/PIIS0960982220314330.pdf"}, {"href": "https://doi.org/10.1016/j.cub.2020.09.063"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Current%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.cub.2020.09.063", "name": "item", "description": "10.1016/j.cub.2020.09.063", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.cub.2020.09.063"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-12-01T00:00:00Z"}}, {"id": "10.1016/j.envres.2024.118880", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:16:26Z", "type": "Journal Article", "created": "2024-04-04", "title": "Unveiling the capacity of bioaugmentation application, in comparison with biochar and rhamnolipid for TPHs degradation in aged hydrocarbons polluted soil", "description": "Persistent, aged hydrocarbons in soil hinder remediation, posing a significant environmental threat. While bioremediation offers an environmentally friendly and cost-effective approach, its efficacy for complex contaminants relies on enhancing pollutant bioavailability. This study explores the potential of immobilized bacterial consortia combined with biochar and rhamnolipids to accelerate bioremediation of aged total petroleum hydrocarbon (TPH)-contaminated soil. Previous research indicates that biochar and biosurfactants can increase bioremediation rates, while mixed consortia offer sequential degradation and higher hydrocarbon mineralization. The present investigation aimed to assess whether combining these strategies could further enhance degradation in aged, complex soil matrices. The bioaugmentation (BA) with bacterial consortium increased the TPHs degradation in aged soil (over 20% compared to natural attenuation - NA). However, co-application of BA with biochar and rhamnolipid higher did not show a statistically prominent synergistic effect. While biochar application facilitated the maintenance of hydrocarbon degrading bacterial consortium in soil, the present study did not identify a direct influence in TPHs degradation. The biochar application in contaminated soil contributed to TPHs adsorption. Rhamnolipid alone slightly increased the TPHs biodegradation with NA, while the combined bioaugmentation treatment with rhamnolipid and biochar increased the degradation between 27.5 and 29.8%. These findings encourage further exploration of combining bioaugmentation with amendment, like biochar and rhamnolipid, for remediating diverse environmental matrices contaminated with complex and aged hydrocarbons.", "keywords": ["Qu\u00edmica agr\u00edcola", "Bioqu\u00edmica", "Biolog\u00eda molecular", "Rhamnolipids", "Molecular biology", "Chemistry", " Inorganic", "Biochemistry", "Qu\u00edmica inorg\u00e1nica", "Hydrocarbons", "Inorganic", "Chemistry", "Biochar", "Soil", "Bioaugmentation", "Agricultural chemistry", "Biodegradation", " Environmental", "Petroleum", "Recalcitrant hydrocarbons", "Charcoal", "Biodegradation", "Soil Pollutants", "TPHs polluted soils", "Glycolipids", "Soil Microbiology"]}, "links": [{"href": "https://doi.org/10.1016/j.envres.2024.118880"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Research", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.envres.2024.118880", "name": "item", "description": "10.1016/j.envres.2024.118880", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.envres.2024.118880"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-01-01T00:00:00Z"}}, {"id": "10.1111/1462-2920.13954", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:19:18Z", "type": "Journal Article", "created": "2022-10-18", "title": "Application of stable-isotope labelling techniques for the detection of active diazotrophs", "description": "Summary

Investigating active participants in the fixation of dinitrogen gas is vital as N is often a limiting factor for primary production. Biological nitrogen fixation is performed by a diverse guild of bacteria and archaea (diazotrophs), which can be free\uffe2\uff80\uff90living or symbionts. Free\uffe2\uff80\uff90living diazotrophs are widely distributed in the environment, yet our knowledge about their identity and ecophysiology is still limited. A major challenge in investigating this guild is inferring activity from genetic data as this process is highly regulated. To address this challenge, we evaluated and improved several 15N\uffe2\uff80\uff90based methods for detecting N2 fixation activity (with a focus on soil samples) and studying active diazotrophs. We compared the acetylene reduction assay and the 15N2 tracer method and demonstrated that the latter is more sensitive in samples with low activity. Additionally, tracing 15N into microbial RNA provides much higher sensitivity compared to bulk soil analysis. Active soil diazotrophs were identified with a 15N\uffe2\uff80\uff90RNA\uffe2\uff80\uff90SIP approach optimized for environmental samples and benchmarked to 15N\uffe2\uff80\uff90DNA\uffe2\uff80\uff90SIP. Lastly, we investigated the feasibility of using SIP\uffe2\uff80\uff90Raman microspectroscopy for detecting 15N\uffe2\uff80\uff90labelled cells. Taken together, these tools allow identifying and investigating active free\uffe2\uff80\uff90living diazotrophs in a highly sensitive manner in diverse environments, from bulk to the single\uffe2\uff80\uff90cell level.

", "keywords": ["Spectrum Analysis", " Raman", "BIOLOGICAL SOIL CRUSTS", "106005 Bioinformatik", "106023 Molekularbiologie", "Nitrogen Fixation", "REVEALS", "FLUORESCENCE", "Research Articles", "Soil Microbiology", "106022 Mikrobiologie", "SPECTROSCOPY", "Bacteria", "Nitrogen Isotopes", "106003 Biodiversity research", "106023 Molecular biology", "GENETIC-REGULATION", "Archaea", "6. Clean water", "SURFACE-ENHANCED RAMAN", "COMMUNITY", "106003 Biodiversit\u00e4tsforschung", "13. Climate action", "Isotope Labeling", "106022 Microbiology", "NITROGEN-FIXATION", "106005 Bioinformatics", "RIBOSOMAL-RNA", "N-2 FIXATION"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.13954"}, {"href": "https://doi.org/10.1111/1462-2920.13954"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Microbiology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/1462-2920.13954", "name": "item", "description": "10.1111/1462-2920.13954", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/1462-2920.13954"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-12-15T00:00:00Z"}}, {"id": "10.1016/j.ijbiomac.2018.12.270", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:16:50Z", "type": "Journal Article", "created": "2019-01-03", "title": "Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions", "description": "Open AccessPolyhydroxyalkanoates (PHAs) production with phototrophic mixed cultures (PMCs) has been recently proposed. These cultures can be selected under the permanent presence of carbon and the PHA production can be enhanced in subsequent accumulation steps. To optimize the PHA production in accumulator reactors, this work evaluated the impact of 1) initial acetate concentration, 2) light intensity, 3) removal of residual nitrogen on the culture performance. Results indicate that low acetate concentration (<30CmM) and specific light intensities around 20W/gX are optimal operating conditions that lead to high polyhydroxybutyrate (PHB) storage yields (0.83+-0.07 Cmol-PHB/Cmol-Acet) and specific PHB production rates of 2.21+-0.07 Cmol-PHB/Cmol X d. This rate is three times higher than previously registered in non-optimized accumulation tests and enabled a PHA content increase from 15 to 30% in less than 4h. Also, it was shown for the first time, the capability of a PMC to use a real waste, fermented cheese whey, to produce PHA with a hydroxyvalerate (HV) content of 12%. These results confirm that fermented wastes can be used as substrates for PHA production with PMCs and that the energy levels in sunlight that lead to specific light intensities from 10 to 20W/gX are sufficient to drive phototrophic PHA production processes.", "keywords": ["0106 biological sciences", "1303 Biochemistry", "Light", "Nitrogen", "Polyhydroxyalkanoates (PHA)", "Fermented cheese whey (FCW)", "Phototrophic mixed cultures (PMCs)", "Acetates", "7. Clean energy", "01 natural sciences", "1315 Structural Biology", "Bioreactors", "Cheese", "Whey", "1312 Molecular Biology", "0105 earth and related environmental sciences", "Polyhydroxyalkanoates", "Volatile fatty acids (VFAs)", "Other Quantitative Biology (q-bio.OT)", "Quantitative Biology - Other Quantitative Biology", "Purple phototrophic bacteria (PPB)", "Kinetics", "Phototrophic Processes", "13. Climate action", "FOS: Biological sciences", "Fermentation"]}, "links": [{"href": "https://doi.org/10.1016/j.ijbiomac.2018.12.270"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Biological%20Macromolecules", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijbiomac.2018.12.270", "name": "item", "description": "10.1016/j.ijbiomac.2018.12.270", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijbiomac.2018.12.270"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-04-01T00:00:00Z"}}, {"id": "10.1016/j.ijbiomac.2019.02.019", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-27T16:16:50Z", "type": "Journal Article", "created": "2019-02-09", "title": "Influence of crosslinker amount on the microstructure and properties of starch-based superabsorbent polymers by one-step preparation at high starch concentration", "description": "This work concerns how crosslinker amount (N, N'-methylene-bisacrylamide) affects the microstructural, absorbent and rheological features of one-step prepared starch-based superabsorbent polymers at a high starch concentration (0.27:1 w/w starch-water). The increased crosslinker amount evidently altered the microstructure and the absorbent and rheological features. Then, the variations in starch-based superabsorbent polymer properties were discussed from a microstructure viewpoint. Particularly, the higher crosslinker quantity rose the crosslinking density and the ratio (GR) of grafted anhydroglucose unit on starch backbone (from 27% to 52%), but short the average polyacrylamide (PAM) chain length (LPAM). These structural features suppressed the chain stretch within starch-based superabsorbent polymer fractal gels (confirmed by smaller Rg value) and promoted the formation of smaller chain networks, thus weakening the water absorption to the starch-based superabsorbent polymer chain networks. Also, the increased GR and reduced LPAM, with lowered chain extension and elevated crosslinking density, probably decreased the flexibility and mobility of chain segments in starch-based superabsorbent polymer gel matrixes. This caused the enhanced robustness and storage modulus of the gels with reduced chain energy dissipation ability.", "keywords": ["Acrylamides", "Water", "Starch", "General Medicine", "Cerium", "02 engineering and technology", "Biochemistry", "01 natural sciences", "0104 chemical sciences", "Absorption", " Physicochemical", "Structural Biology", "Rheology", "0210 nano-technology", "Molecular Biology"]}, "links": [{"href": "https://doi.org/10.1016/j.ijbiomac.2019.02.019"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Biological%20Macromolecules", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijbiomac.2019.02.019", "name": "item", "description": "10.1016/j.ijbiomac.2019.02.019", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijbiomac.2019.02.019"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-05-01T00:00:00Z"}}, {"id": "10.1016/j.ijbiomac.2019.07.007", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:16:50Z", "type": "Journal Article", "created": "2019-07-02", "title": "Effect of drying temperature on structural and thermomechanical properties of konjac glucomannan-zein blend films", "description": "Konjac glucomannan (KGM)/zein blend films were successfully prepared by solution casting at different drying temperatures (40, 50, 60, 70 and 80\u00b0C). The effects of drying temperature on the films' structural, thermomechanical, mechanical and water barrier properties were investigated. Microstructural observations indicated that zein particles were homogeneously dispersed in KGM continuous matrix, and the blend film dried at 60\u00b0C showed the most compact and smooth surface. Dynamic mechanical thermal analysis curves showed that with increasing drying temperature from 40 to 60\u00b0C, glass transition temperature (Tg) of films increased; however, with further increase in temperature, the Tg decreased, indicating the compatibility of film components was the highest when dried at 60\u00b0C. The hydrophobicity of blend film dried at 60\u00b0C was significantly stronger than that of other blend films, supported by the highest water contact angle, and the lowest swelling ratio and solubility. Moreover, the film dried at 60\u00b0C showed the highest tensile strength, elongation at break, and the lowest water vapor permeability. Therefore 60\u00b0C was preferred for KGM/zein blend film preparation. This study indicated that intermolecular interactions among film components were greatly influenced by the drying temperature, and should be carefully noticed for film preparation.", "keywords": ["Zein", "Temperature", "General Medicine", "04 agricultural and veterinary sciences", "Biochemistry", "Permeability", "Mannans", "Steam", "0404 agricultural biotechnology", "Solubility", "Structural Biology", "Desiccation", "0405 other agricultural sciences", "Molecular Biology", "Hydrophobic and Hydrophilic Interactions", "Mechanical Phenomena"]}, "links": [{"href": "https://doi.org/10.1016/j.ijbiomac.2019.07.007"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/International%20Journal%20of%20Biological%20Macromolecules", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.ijbiomac.2019.07.007", "name": "item", "description": "10.1016/j.ijbiomac.2019.07.007", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.ijbiomac.2019.07.007"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-10-01T00:00:00Z"}}, {"id": "10.1038/s41467-020-15622-0", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:18:21Z", "type": "Journal Article", "created": "2020-05-05", "title": "Sustained fluvial deposition recorded in Mars\u2019 Noachian stratigraphic record", "description": "Abstract

Orbital observation has revealed a rich record of fluvial landforms on Mars, with much of this record dating 3.6\uffe2\uff80\uff933.0 Ga. Despite widespread geomorphic evidence, few analyses of Mars\uffe2\uff80\uff99 alluvial sedimentary-stratigraphic record exist, with detailed studies of alluvium largely limited to smaller sand-bodies amenable to study in-situ by rovers. These typically metre-scale outcrop dimensions have prevented interpretation of larger scale channel-morphology and long-term basin evolution, vital for understanding the past Martian climate. Here we give an interpretation of a large sedimentary succession at Izola mensa within the NW Hellas Basin rim. The succession comprises channel and barform packages which together demonstrate that river deposition was already well established >3.7 Ga. The deposits mirror terrestrial analogues subject to low-peak discharge variation, implying that river deposition at Izola was subject to sustained, potentially perennial, fluvial flow. Such conditions would require an environment capable of maintaining large volumes of water for extensive time-periods, necessitating a precipitation-driven hydrological cycle.

", "keywords": ["550", "Science", "General Biochemistry", "Genetics and Molecular Biology", "Q", "500", "General Physics and Astronomy", "Geomorphology", "General Chemistry", "15. Life on land", "01 natural sciences", "Article", "12. Responsible consumption", "Sedimentology", " Stratigraphy", " Fluvial Deposits", " Mars", " Sedimentary Deposits", "[SDU.STU.PL]Sciences of the Universe [physics]/Earth Sciences/Planetology", "13. Climate action", "Inner planets", "[SDU.STU.PL] Sciences of the Universe [physics]/Earth Sciences/Planetology", "0105 earth and related environmental sciences"]}, "links": [{"href": "http://oro.open.ac.uk/70442/1/70442.pdf"}, {"href": "https://www.nature.com/articles/s41467-020-15622-0.pdf"}, {"href": "https://doi.org/10.1038/s41467-020-15622-0"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-020-15622-0", "name": "item", "description": "10.1038/s41467-020-15622-0", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-020-15622-0"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-05T00:00:00Z"}}, {"id": "10.1016/j.nbt.2019.10.010", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:17:03Z", "type": "Journal Article", "created": "2019-11-12", "title": "Advances in combined enzymatic extraction of ferulic acid from wheat bran", "description": "Wheat bran could be utilised as feedstock for innovative and sustainable biorefinery processes. Here, an enzymatic hydrolysis process for ferulic acid (FA) extraction was optimised step by step for total wheat bran (Tritello) and then also applied to the outer bran layer (Bran 1). Proteins, reducing sugars, total phenols and FA were quantified. The highest FA yields (0.82-1.05\u202fg/kg bran) were obtained either by rehydrating the bran by autoclaving (Tritello) or by steam explosion (Bran 1) using a bran/water ratio of 1:20, followed by enzymatic pre-treatment with Alcalase and Termamyl, to remove protein and sugars, and a final enzymatic hydrolysis with Pentopan and feruloyl esterase to solubilise phenol. FA was recovered from the final digestate via solid phase extraction. A 40-fold scale-up was also performed and the release of compounds along all the process steps and at increasing incubation times was monitored. Results showed that FA was initially present at a minimum level while it was specifically released during the enzymatic treatment. In the final optimized process, the FA extraction yield was higher than that obtained with NaOH control hydrolysis while, in comparison with other FA enzymatic extraction methods, fewer process steps were required and no buffers, strong acid/alkali nor toxic compounds were used. Furthermore, the proposed process may be easily scaled-up, confirming the feasibility of wheat bran valorisation by biorefinery processes to obtain valuable compounds having several areas of potential industrial exploitation.", "keywords": ["Dietary Fiber", "0106 biological sciences", "0301 basic medicine", "Coumaric Acids", "Bioengineering", "Wheat bran", "01 natural sciences", "Bio-based Industries Innovation action - Demonstration", "Enzymatic extraction", "03 medical and health sciences", "Subtilisins", "European Commission", "Molecular Biology", "Knowmad Institut", "2. Zero hunger", "EC", "Hydrolysis", "H2020", "Polyphenols", "General Medicine", "Ferulic acid", "Biorefinery", "Biorefinery; Enzymatic extraction; Ferulic acid; Polyphenols; Steam explosion; Wheat bran", "Steam explosion", "alpha-Amylases", "Biotechnology"]}, "links": [{"href": "https://cris.unibo.it/bitstream/11585/706995/1/Ferri%20et%20al%202020%20New%20Biotech.pdf"}, {"href": "https://doi.org/10.1016/j.nbt.2019.10.010"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.nbt.2019.10.010", "name": "item", "description": "10.1016/j.nbt.2019.10.010", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.nbt.2019.10.010"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-05-01T00:00:00Z"}}, {"id": "10.1016/j.nbt.2023.12.003", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:17:03Z", "type": "Journal Article", "created": "2023-12-19", "title": "Phytostabilization of metal(loid)s by ten emergent macrophytes following a 90-day exposure to industrially contaminated groundwater", "description": "Better understanding of macrophyte tolerance under long exposure times in real environmental matrices is crucial for phytoremediation and phytoattenuation strategies for aquatic systems. The metal(loid) attenuation ability of 10 emergent macrophyte species (Carex riparia, Cyperus longus, Cyperus rotundus, Iris pseudacorus, Juncus effusus, Lythrum salicaria, Menta aquatica, Phragmites australis, Scirpus holoschoenus, and Typha angustifolia) was investigated using real groundwater from an industrial site, over a 90-day exposure period. A 'phytobial' treatment was included, with 3 plant growth-promoting rhizobacterial strains. Plants exposed to the polluted water generally showed similar or reduced aerial biomass compared to the controls, except for C. riparia. This species, along with M. aquatica, exhibited improved biomass after bioaugmentation. Phytoremediation mechanisms accounted for more than 60% of As, Cd, Cu, Ni, and Pb removal, whilst abiotic mechanisms contributed to \u223c80% removal of Fe and Zn. Concentrations of metal(loid)s in the roots were generally between 10-100 times higher than in the aerial parts. The macrophytes in this work can be considered 'underground attenuators', more appropriate for rhizostabilization strategies, especially L. salicaria, M. aquatica, S. holoschoenus, and T. angustifolia. For I. pseudacorus, C. longus, and C. riparia; harvesting the aerial parts could be a complementary phytoextraction approach to further remove Pb and Zn. Of all the plants, S. holoschoenus showed the best balance between biomass production and uptake of multiple metal(loid)s. Results also suggest that multiple phytostrategies may be possible for the same plant depending on the final remedial aim. Phytobial approaches need to be further assessed for each macrophyte species.", "keywords": ["Rhizostabilization", "Metalloid", "Biotecnolog\u00eda", "Biolog\u00eda molecular", "Emergent macrophyte", "Metal", "Molecular biology", "Plants", "15. Life on land", "Poaceae", "6. Clean water", "Biodegradation", " Environmental", "Lead", "13. Climate action", "Metals", " Heavy", "Plant growth promoting bacteria", "Biomass", "Groundwater", "Biotechnology"]}, "links": [{"href": "https://doi.org/10.1016/j.nbt.2023.12.003"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/New%20Biotechnology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.nbt.2023.12.003", "name": "item", "description": "10.1016/j.nbt.2023.12.003", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.nbt.2023.12.003"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-03-01T00:00:00Z"}}, {"id": "10.1016/j.soilbio.2022.108918", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:17:30Z", "type": "Journal Article", "created": "2022-12-22", "title": "Litter chemistry of common European tree species drives the feeding preference and consumption rate of soil invertebrates, and shapes the diversity and structure of gut and faecal microbiomes", "description": "Open AccessTerrestrial isopods and millipedes are key drivers of a litter decomposition in terrestrial ecosystems but the effects of litter chemistry on feeding preference and litter consumption rate as well as on the diversity and composition of gut and faecal microbiome still entails several challenges. We established a mesocosm experiment with terrestrial isopods (Oniscus asellus) and millipedes (Glomeris marginata) fed by leaf litter from six common European tree species (ash, maple, lime, beech, oak and Norway spruce) to reveal the effect of litter chemistry on consumption rate and feeding preference as well as on the compositions of gut and faecal microbiomes. The total percentage of consumed litter showed that O. asellus preferred nutrient-rich and labile-C litter of ash over more recalcitrant litter of oak, beech, and Norway spruce, while G. marginata preferred calcium-rich ash, maple and lime litter over beech and Norway spruce. Consumption of litter by O. asellus and G. marginata increased with concentrations of magnesium, sulphur and potassium but decreased with concentrations of iron, phosphorus, lignin, cellulose and TOC. The millipede G. marginata harboured higher bacterial OTU richness (73.5 \u00b1 12.5) than the isopod O. asellus (49.1 \u00b1 15.9), but fungal OTU richness was similar with 25.8 \u00b1 6.7 in O. asellus and 25.7 \u00b1 2.7 in G. marginata. In total, faeces of both animals hosted higher diversity than gut. In gut and faeces of O. asellus, the fungal OTU richness was highest for individuals fed by litter of Norway spruce, while lowest OTU richness was recorded for individuals fed by litter of more palatable ash. In contrast, the highest diversity of the fungal community in gut and faeces of G. marginata was recorded for individuals fed by palatable lime litter, while the lowest OTUs richness was recorded when millipedes were fed by maple and spruce. The structures of bacterial and fungal communities generally separated between O. asellus and G. marginata. The fungal community structure in gut and faeces differed between animals fed by different foliar litters, while the bacterial community structure mainly differed between gut and faeces regardless of the offered type of litter. The fungal community structure in gut and faeces of O. asellus and G. marginata were shaped by concentrations of magnesium, sulphur, lignin and cellulose. The bacterial communities in gut and faeces of both O. asellus and G. marginata were dominated by copiotrophic bacteria, while fungal communities were dominated by unspecified saprotrophs. Our study suggest that litter quality is a strong driver of feeding preference and consumption rates as well as composition of bacterial and fungal communities in gut and faeces of two species representing the main groups of litter feeding soil fauna in European forests.", "keywords": ["0301 basic medicine", "Genomic Insights into Social Insects and Symbiosis", "Plant Science", "Plant litter", "Agricultural and Biological Sciences", "03 medical and health sciences", "Biochemistry", " Genetics and Molecular Biology", "Litter", "Genetics", "Ecological Niche", "Biology", "Ecosystem", "Beech", "0303 health sciences", "Species Distribution Modeling and Climate Change Impacts", "Ecology", "Ecological Modeling", "Botany", "Life Sciences", "15. Life on land", "Plant-Parasitic Nematodes in Molecular Plant Pathology", "Detritus", "FOS: Biological sciences", "Detritivore", "Environmental Science", "Physical Sciences", "Species richness"]}, "links": [{"href": "https://doi.org/10.1016/j.soilbio.2022.108918"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1016/j.soilbio.2022.108918", "name": "item", "description": "10.1016/j.soilbio.2022.108918", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1016/j.soilbio.2022.108918"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-02-01T00:00:00Z"}}, {"id": "10.1021/acs.est.0c05203", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-27T16:17:56Z", "type": "Journal Article", "created": "2021-02-08", "title": "Mercury Reduction by Nanoparticulate Vivianite", "description": "Open AccessISSN:0013-936X", "keywords": ["abiotic Hg II reduction", "Chemical Sciences not elsewhere classified", "Physiology", "0211 other engineering and technologies", "02 engineering and technology", "Hg 0", "Hg II", "PO", "01 natural sciences", "Phosphates", "Environmental Sciences not elsewhere classified", "Ferrous Compounds", "Hg II reducers", "Molecular Biology", "ferrous iron phosphate mineral vivi.", "Ecosystem", "0105 earth and related environmental sciences", "Pharmacology", "Fe II content", "Ecology", "Nanoparticulate Vivianite Mercury", "Cell Biology", "Mercury", "6. Clean water", "Fe II 3", "13. Climate action", "Oxidation-Reduction", "Biological Sciences not elsewhere classified"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.0c05203"}, {"href": "https://doi.org/10.1021/acs.est.0c05203"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.est.0c05203", "name": "item", "description": "10.1021/acs.est.0c05203", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.est.0c05203"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-08T00:00:00Z"}}, {"id": "10.1021/acs.est.0c06687", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:17:56Z", "type": "Journal Article", "created": "2021-02-10", "title": "Microbial Interactions Drive the Complete Catabolism of the Antibiotic Sulfamethoxazole in Activated Sludge Microbiomes", "description": "Microbial communities are believed to outperform monocultures in the complete catabolism of organic pollutants via reduced metabolic burden and increased robustness to environmental challenges; however, the interaction mechanism in functional microbiomes remains poorly understood. Here, three functionally differentiated activated sludge microbiomes (S1: complete catabolism of sulfamethoxazole (SMX); S2: complete catabolism of the phenyl part of SMX ([phenyl]-SMX) with stable accumulation of its heterocyclic product 3-amino-5-methylisoxazole (3A5MI); A: complete catabolism of 3A5MI rather than [phenyl]-SMX) were enriched. Combining time-series cultivation-independent microbial community analysis, DNA-stable isotope probing, molecular ecological network analysis, and cultivation-dependent function verification, we identified key players involved in the SMX degradation process. Paenarthrobacter and Nocardioides were primary degraders for the initial cleavage of the sulfonamide functional group (-C-S-N- bond) and 3A5MI degradation, respectively. Complete catabolism of SMX was achieved by their cross-feeding. The co-culture of Nocardioides, Acidovorax, and Sphingobium demonstrated that the nondegraders Acidovorax and Sphingobium were involved in the enhancement of 3A5MI degradation. Moreover, we unraveled the internal labor division patterns and connections among the active members centered on the two primary degraders. Overall, the proposed methodology is promisingly applicable and would help generate mechanistic, predictive, and operational understanding of the collaborative biodegradation of various contaminants. This study provides useful information for synthetic activated sludge microbiomes with optimized environmental functions.", "keywords": ["Sulfamethoxazole", "Physiology", "Science Policy", "analysis", "0211 other engineering and technologies", "02 engineering and technology", "Microbiology", "Environmental Sciences not elsewhere classified", "heterocyclic product 3-", "11. Sustainability", "Activated Sludge Microbiomes Microb.", "Acidovorax", "SMX degradation process", "Molecular Biology", "cultivation-dependent function veri.", "phenyl", "Ecology", "Sewage", "Microbiota", "catabolism", "Nocardioide", "Computational Biology", "Cell Biology", "6. Clean water", "Sphingobium", "Anti-Bacterial Agents", "sludge microbiomes", "Infectious Diseases", "Complete", "Biodegradation", " Environmental", "Microbial Interactions Drive", "degrader", "Microbial Interactions", "labor division patterns", "5MI degradation", "Water Pollutants", " Chemical", "Developmental Biology", "Biological Sciences not elsewhere classified"]}, "links": [{"href": "https://pubs.acs.org/doi/pdf/10.1021/acs.est.0c06687"}, {"href": "https://doi.org/10.1021/acs.est.0c06687"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Environmental%20Science%20%26amp%3B%20Technology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1021/acs.est.0c06687", "name": "item", "description": "10.1021/acs.est.0c06687", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1021/acs.est.0c06687"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-02-10T00:00:00Z"}}, {"id": "10.1038/s41396-018-0335-2", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:18:19Z", "type": "Journal Article", "created": "2019-01-03", "title": "Ant colonies promote the diversity of soil microbial communities", "description": "Abstract

Little is known about the role of ant colonies in regulating the distribution and diversity of soil microbial communities across large spatial scales. Here, we conducted a survey across &gt;1000\uffe2\uff80\uff89km in eastern Australia and found that, compared with surrounding bare soils, ant colonies promoted the richness (number of phylotypes) and relative abundance of rare taxa of fungi and bacteria. Ant nests were also an important reservoir for plant pathogens. Our study also provides a portfolio of microbial phylotypes only found in ant nests, and which are associated with high nutrient availability. Together, our work highlights the fact that ant nests are an important refugia for microbial diversity.Warming in the Arctic accelerates thawing of permafrost-affected soils, which leads to a release of greenhouse gases to the atmosphere. We do not know whether permafrost thaw also releases non-methane volatile organic compounds that can contribute to both negative and positive radiative forcing on climate. Here we show using proton transfer reaction\uffe2\uff80\uff93time of flight\uffe2\uff80\uff93mass spectrometry that substantial amounts of ethanol and methanol and in total 316 organic ions were released from Greenlandic permafrost soils upon thaw in laboratory incubations. We demonstrate that the majority of this release is taken up in the active layer above. In an experiment using 14C-labeled ethanol and methanol, we demonstrate that these compounds are consumed by microorganisms. Our findings highlight that the thawing permafrost soils are not only a considerable source of volatile organic compounds but also that the active layer regulates their release into the atmosphere.

", "keywords": ["0301 basic medicine", "Ethanol", "Science", "Climate", "Methanol", "General Biochemistry", "Genetics and Molecular Biology", "Q", "General Physics and Astronomy", "Permafrost", "General Chemistry", "15. Life on land", "01 natural sciences", "Article", "03 medical and health sciences", "13. Climate action", "11. Sustainability", "SDG 13 - Climate Action", "Soil Microbiology", "0105 earth and related environmental sciences"]}, "links": [{"href": "https://www.nature.com/articles/s41467-018-05824-y.pdf"}, {"href": "https://doi.org/10.1038/s41467-018-05824-y"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-018-05824-y", "name": "item", "description": "10.1038/s41467-018-05824-y", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-018-05824-y"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2018-08-24T00:00:00Z"}}, {"id": "10.1038/s41467-018-05980-1", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:18:20Z", "type": "Journal Article", "created": "2018-08-29", "title": "Land use driven change in soil pH affects microbial carbon cycling processes", "description": "Abstract

Soil microorganisms act as gatekeepers for soil\uffe2\uff80\uff93atmosphere carbon exchange by balancing the accumulation and release of soil organic matter. However, poor understanding of the mechanisms responsible hinders the development of effective land management strategies to enhance soil carbon storage. Here we empirically test the link between microbial ecophysiological traits and topsoil carbon content across geographically distributed soils and land use contrasts. We discovered distinct pH controls on microbial mechanisms of carbon accumulation. Land use intensification in low-pH soils that increased the pH above a threshold (~6.2) leads to carbon loss through increased decomposition, following alleviation of acid retardation of microbial growth. However, loss of carbon with intensification in near-neutral pH soils was linked to decreased microbial biomass and reduced growth efficiency that was, in turn, related to trade-offs with stress alleviation and resource acquisition. Thus, less-intensive management practices in near-neutral pH soils have more potential for carbon storage through increased microbial growth efficiency, whereas in acidic soils, microbial growth is a bigger constraint on decomposition rates.Humans modify ecosystems and biodiversity worldwide, with negative consequences for ecosystem functioning. Promoting plant diversity is increasingly suggested as a mitigation strategy. However, our mechanistic understanding of how plant diversity affects the diversity of heterotrophic consumer communities remains limited. Here, we disentangle the relative importance of key components of plant diversity as drivers of herbivore, predator, and parasitoid species richness in experimental forests and grasslands. We find that plant species richness effects on consumer species richness are consistently positive and mediated by elevated structural and functional diversity of the plant communities. The importance of these diversity components differs across trophic levels and ecosystems, cautioning against ignoring the fundamental ecological complexity of biodiversity effects. Importantly, plant diversity effects on higher trophic-level species richness are in many cases mediated by modifications of consumer abundances. In light of recently reported drastic declines in insect abundances, our study identifies important pathways connecting plant diversity and consumer diversity across ecosystems.

", "keywords": ["0106 biological sciences", "2. Zero hunger", "570", "/dk/atira/pure/core/keywords/nachhaltigkeitswissenschaft; name=Sustainability Science", "Science", "/dk/atira/pure/subjectarea/asjc/1600; name=Chemistry(all)", "Q", "/dk/atira/pure/subjectarea/asjc/1300; name=Biochemistry", " Genetics and Molecular Biology(all)", "634", "Biodiversity", "/dk/atira/pure/core/keywords/biology; name=Ecosystems Research", "Plants", "15. Life on land", "/dk/atira/pure/subjectarea/asjc/1000; name=General", "01 natural sciences", "Article", "ddc:", "/dk/atira/pure/subjectarea/asjc/3100; name=Physics and Astronomy(all)", "Species Specificity", "Animals", "14. Life underwater", "Arthropods"]}, "links": [{"href": "https://www.nature.com/articles/s41467-019-09448-8.pdf"}, {"href": "https://doi.org/10.1038/s41467-019-09448-8"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Nature%20Communications", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41467-019-09448-8", "name": "item", "description": "10.1038/s41467-019-09448-8", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41467-019-09448-8"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2019-03-29T00:00:00Z"}}, {"id": "10.1038/s41467-022-29161-3", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:18:21Z", "type": "Journal Article", "created": "2022-03-17", "title": "Structure and function of the soil microbiome underlying N2O emissions from global wetlands", "description": "Abstract

Wetland soils are the greatest source of nitrous oxide (N2O), a critical greenhouse gas and ozone depleter released by microbes. Yet, microbial players and processes underlying the N2O emissions from wetland soils are poorly understood. Using in situ N2O measurements and by determining the structure and potential functional of microbial communities in 645 wetland soil samples globally, we examined the potential role of archaea, bacteria, and fungi in nitrogen (N) cycling and N2O emissions. We show that N2O emissions are higher in drained and warm wetland soils, and are correlated with functional diversity of microbes. We further provide evidence that despite their much lower abundance compared to bacteria, nitrifying archaeal abundance is a key factor explaining N2O emissions from wetland soils globally. Our data suggest that ongoing global warming and intensifying environmental change may boost archaeal nitrifiers, collectively transforming wetland soils to a greater source of N2O.Diversified cropping systems, especially those including legumes, have been proposed to enhance food production with reduced inputs and environmental impacts. However, the impact of legume pre-crops on main crop yield and its drivers has never been systematically investigated in a global context. Here, we synthesize 11,768 yield observations from 462 field experiments comparing legume-based and non-legume cropping systems and show that legumes enhanced main crop yield by 20%. These yield advantages decline with increasing N fertilizer rates and crop diversity of the main cropping system. The yield benefits are consistent among main crops (e.g., rice, wheat, maize) and evident across pedo-climatic regions. Moreover, greater yield advantages (32% vs. 7%) are observed in low- vs. high-yielding environments, suggesting legumes increase crop production with low inputs (e.g., in Africa or organic agriculture). In conclusion, our study suggests that legume-based rotations offer a critical pathway for enhancing global crop production, especially when integrated into low-input and low-diversity agricultural systems.Magnesium is the metal at the center of all types of chlorophyll and is thus crucial to photosynthesis. When an element is involved in a biosynthetic pathway its isotopes are fractionated based on the difference of vibrational frequency between the different molecules. With the technical advance of multi-collectors plasma-mass-spectrometry and improvement in analytical precision, it has recently been found that two types of chlorophylls (a and b) are isotopically distinct. These results have very significant implications with regards to the use of Mg isotopes to understand the biosynthesis of chlorophyll. Here we present theoretical constraints on the origin of these isotopic fractionations through ab initio calculations. We present the fractionation factor for chlorphyll a, b, d, and f. We show that the natural isotopic variations among chlorophyll a and b are well explained by isotopic fractionation under equilibrium, which implies exchanges of Mg during the chlorophyll cycle. We predict that chlorophyll d and f should be isotopically fractionated compared to chlorophyll a and that this could be used in the future to understand the biosynthesis of these molecules.

", "keywords": ["Chlorophyll", "0301 basic medicine", "0303 health sciences", "RELEVANT", "Molecular Structure", "PHOTOSYNTHESIS", "Science", "Q", "POTENTIALS", "R", "Chemical Fractionation", "Fe", "Article", "3. Good health", "[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry", "[CHIM.THEO] Chemical Sciences/Theoretical and/or physical chemistry", "03 medical and health sciences", "Isotopes", "Zn", "Medicine", "PLANTS", "Magnesium", "[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry", " Molecular Biology/Biochemistry [q-bio.BM]", "GEOCHEMISTRY", "[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry", " Molecular Biology/Biochemistry [q-bio.BM]"], "contacts": [{"organization": "Fr\u00e9d\u00e9ric Moynier, Fr\u00e9d\u00e9ric Moynier, Toshiyuki Fujii,", "roles": ["creator"]}]}, "links": [{"href": "https://www.nature.com/articles/s41598-017-07305-6.pdf"}, {"href": "https://doi.org/10.1038/s41598-017-07305-6"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Scientific%20Reports", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1038/s41598-017-07305-6", "name": "item", "description": "10.1038/s41598-017-07305-6", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1038/s41598-017-07305-6"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2017-08-01T00:00:00Z"}}, {"id": "10.1038/s41598-023-49194-y", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:18:27Z", "type": "Journal Article", "created": "2023-12-13", "title": "Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis", "description": "Abstract

Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16\uffe2\uff80\uff93C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892\uffc2\uffa0bp, with a total of 3474 coding sequences and a G\uffe2\uff80\uff89+\uffe2\uff80\uff89C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1\uffe2\uff80\uff934 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16\uffe2\uff80\uff93C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.Trace metal elements are essential for plant growth but become toxic at high concentrations, while some non-essential elements, such as Cd and As, show toxicity even in traces. Thus, metal homeostasis is tightly regulated in plants. Plant species colonising metalliferous soils have evolved mechanisms to hypertolerate metals and, in rare cases, can hyperaccumulate them in excess amounts in their shoots. The molecular mechanisms of metal hypertolerance and hyperaccumulation are likely derived from alterations in the basic mechanisms involved in general metal homeostasis. Genes involved in metal transport, synthesis of metal chelators and oxidative stress responses are constitutively and highly expressed in metal hypertolerant and hyperaccumulator species. Plant specialized metabolites and cell wall components have been proposed as major players in these mechanisms. In addition, the high intra-specific natural variation of metal hypertolerance and hyperaccumulation suggests that various molecular mechanisms might be involved in the evolution of these traits. To date, the potential of wild plant populations as systems to study metal tolerance and hyperaccumulation has not been fully exploited. The advent of next-generation sequencing (NGS) has enabled the study of non-model species, providing an opportunity to study natural populations and new tolerant and/or hyperaccumulating species, and will provide new insights into metal tolerance and hyperaccumulation. In this review we highlight background knowledge about metal tolerance and hyperaccumulation in plants and the current state-of-the-art techniques to study and identify the underlying mechanisms of metal hypertolerance and hyperaccumulation. We also outline for the reader the importance of the multidisciplinarity of this research field and how the integration of multiomic approaches will benefit facing the future scientific challenges.&lt;p&gt;Plant litter chemistry is altered during decomposition but it remains unknown if these alterations, and thus the composition of residual litter, will change in response to climate. Selective microbial mineralization of litter components and the accumulation of microbial necromass can drive litter compositional change, but the extent to which these mechanisms respond to climate remains poorly understood. We addressed this knowledge gap by studying needle litter decomposition along a boreal forest climate transect. Specifically, we investigated how the composition and/or metabolism of the decomposer community varies with climate, and if that variation is associated with distinct modifications of litter chemistry during decomposition. We analyzed the composition of microbial phospholipid fatty acids (PLFAs) in the litter layer and measured natural abundance &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C&lt;sub&gt;PLFA&lt;/sub&gt; values as an integrated measure of microbial metabolisms. Changes in litter chemistry and &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C values were measured in litterbag experiments conducted at each transect site. A warmer climate was associated with higher litter nitrogen concentrations as well as altered microbial community structure (lower fungi:bacteria ratios) and microbial metabolism (higher &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C&lt;sub&gt;PLFA&lt;/sub&gt;). Litter in warmer transect regions accumulated less aliphatic&amp;#8208;C (lipids, waxes) and retained more O&amp;#8208;alkyl&amp;#8208;C (carbohydrates), consistent with enhanced &lt;sup&gt;13&lt;/sup&gt;C&amp;#8208;enrichment in residual litter, than in colder regions. These results suggest that chemical changes during litter decomposition will change with climate, driven primarily by indirect climate effects (e.g., greater nitrogen availability and decreased fungi:bacteria ratios) rather than direct temperature effects. A positive correlation between microbial biomass &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C values and &lt;sup&gt;13&lt;/sup&gt;C&amp;#8208;enrichment during decomposition suggests that change in litter chemistry is driven more by distinct microbial necromass inputs than differences in the selective removal of litter components. Our study highlights the role that microbial inputs during early litter decomposition can play in shaping surface litter contribution to soil organic matter as it responds to climate warming effects such as greater nitrogen availability.&lt;/p&gt;

", "keywords": ["DECOMPOSITION", "C-13", "CP‐", "necromass", "litter decomposition", "COMMUNITY COMPOSITION", "Soil", "CARBON SEQUESTRATION", "Taiga", "boreal forest", "bacteria", "C-13 NMR", "TEMPERATURE", "Biochemistry", " cell and molecular biology", "Soil Microbiology", "FUNGAL", "2. Zero hunger", "MAS C-13‐", "Fungi", "04 agricultural and veterinary sciences", "15. Life on land", "NMR", "6. Clean water", "climate transect", "Plant Leaves", "13. Climate action", "FOREST SOILS", "PLFA", "0401 agriculture", " forestry", " and fisheries", "fungi", "FATTY-ACIDS", "BULK CARBON", "LIGNIN"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.15420"}, {"href": "https://doi.org/10.1111/gcb.15420"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/gcb.15420", "name": "item", "description": "10.1111/gcb.15420", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/gcb.15420"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-11-16T00:00:00Z"}}, {"id": "10.1093/bioinformatics/btad407", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:19:03Z", "type": "Journal Article", "created": "2023-06-24", "title": "enviRule: an end-to-end system for automatic extraction of reaction patterns from environmental contaminant biotransformation pathways", "description": "Abstract Motivation

Transformation products (TPs) of man-made chemicals, formed through microbially mediated transformation in the environment, can have serious adverse environmental effects, yet the analytical identification of TPs is challenging. Rule-based prediction tools are successful in predicting TPs, especially in environmental chemistry applications that typically have to rely on small datasets, by imparting the existing knowledge on enzyme-mediated biotransformation reactions. However, the rules extracted from biotransformation reaction databases usually face the issue of being over/under-generalized and are not flexible to be updated with new reactions.

Results

We developed an automatic rule extraction tool called enviRule. It clusters biotransformation reactions into different groups based on the similarities of reaction fingerprints, and then automatically extracts and generalizes rules for each reaction group in SMARTS format. It optimizes the genericity of automatic rules against the downstream TP prediction task. Models trained with automatic rules outperformed the models trained with manually curated rules by 30% in the area under curve (AUC) scores. Moreover, automatic rules can be easily updated with new reactions, highlighting enviRule\uffe2\uff80\uff99s strengths for both automatic extraction of optimized reactions rules and automated updating thereof.

Availability and implementation

enviRule code is freely available at https://github.com/zhangky12/enviRule.

", "keywords": ["10120 Department of Chemistry", "Statistics and Probability", "Original Paper", "1303 Biochemistry", "Computational Biology", "Biochemistry", "Computer Science Applications", "Computational Mathematics", "Computational Theory and Mathematics", "13. Climate action", "540 Chemistry", "1312 Molecular Biology", "1706 Computer Science Applications", "2613 Statistics and Probability", "2605 Computational Mathematics", "Molecular Biology", "Biotransformation", "1703 Computational Theory and Mathematics"]}, "links": [{"href": "https://doi.org/10.1093/bioinformatics/btad407"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Bioinformatics", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1093/bioinformatics/btad407", "name": "item", "description": "10.1093/bioinformatics/btad407", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1093/bioinformatics/btad407"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-06-24T00:00:00Z"}}, {"id": "10.1093/femsle/fnab100", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:19:04Z", "type": "Journal Article", "created": "2021-07-30", "title": "Transcriptomic markers of fungal growth, respiration and carbon-use efficiency", "description": "ABSTRACT

Fungal metabolic carbon acquisition and its subsequent partitioning between biomass production and respiration, i.e. the carbon-use efficiency (CUE), are central parameters in biogeochemical modeling. However, current available techniques for estimating these parameters are all associated with practical and theoretical shortcomings, making assessments unreliable. Gene expression analyses hold the prospect of phenotype prediction by indirect means, providing new opportunities to obtain information about metabolic priorities. We cultured four different fungal isolates (Chalara longipes, Laccaria bicolor, Serpula lacrymans and Trichoderma harzianum) in liquid media with contrasting nitrogen availability and measured growth rates and respiration to calculate CUE. By relating gene expression markers to measured carbon fluxes, we identified genes coding for 1,3-\uffce\uffb2-glucan synthase and 2-oxoglutarate dehydrogenase as suitable markers for growth and respiration, respectively, capturing both intraspecific variation as well as within-strain variation dependent on growth medium. A transcript index based on these markers correlated significantly with differences in CUE between the fungal isolates. Our study paves the way for the use of these markers to assess differences in growth, respiration and CUE in natural fungal communities, using metatranscriptomic or the RT-qPCR approach.Retreating glaciers, icons of climate change, release new potential habitats for both aquatic and terrestrial organisms. High\uffe2\uff80\uff90elevation species are threatened by temperature increases and the upward migration of lowlands species. Improving our understanding of successional processes after glacier retreat becomes urgent, especially in the tropics, where glacier shrinkage is particularly fast. We examined the successional patterns of aquatic invertebrates, ground beetles, terrestrial plants, soil eukaryotes (algae, invertebrates, plants) in an equatorial glacier foreland (Carihuairazo, Ecuador). Based on both taxonomical identification and eDNA metabarcoding, we analysed the effects of both environmental conditions and age of deglacierization on community composition. Except for algae, diversity increased with time since deglacierization, especially among passive dispersers, suggesting that dispersal was a key driver structuring the glacier foreland succession. Spatial \uffce\uffb2\uffe2\uff80\uff90diversity was mainly attributed to nestedness for aquatic invertebrates, terrestrial plants and soil algae, likely linked to low environmental variability within the studied glacier foreland; and to turnover for soil invertebrates, suggesting competition exclusion at the oldest successional stage. Pioneer communities were dominated by species exhibiting flexible feeding strategies and high dispersal ability (mainly transported by wind), probably colonising from lower altitudes, or from the glacier in the case of algae. Overall, glacier foreland colonisation in the tropics exhibit common characteristics to higher latitudes. High\uffe2\uff80\uff90elevation species are nevertheless threatened, as the imminent extinction of many tropical glaciers will affect species associated to glacier\uffe2\uff80\uff90influenced habitats but also prevent cold\uffe2\uff80\uff90adapted and hygrophilous species from using these habitats as refuges in a warming world.

", "keywords": ["Colonization", "[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics", "550", "Early succession", "glacier retreat", "Sociology", "[SDV.EE.ECO] Life Sciences [q-bio]/Ecology", " environment/Ecosystems", "Environmental DNA Sequencing", "Glacier", "Ecology", "Geography", "early succession", "Life Sciences", "Phylogenetics and taxonomy", "Biodiversity", "[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics", "Threatened species", "FOS: Sociology", "Multiple-taxa", "multiple-taxa", "Habitat", "[SDE]Environmental Sciences", "Physical Sciences", "environment/Ecosystems", "570", "Physical geography", "Population", "Global Diversity of Microbial Eukaryotes and Their Evolution", "[SDV.BV.BOT] Life Sciences [q-bio]/Vegetal Biology/Botanics", "Ecological succession", "Biochemistry", " Genetics and Molecular Biology", "Biological dispersal", "[SDV.BID.SPT] Life Sciences [q-bio]/Biodiversity/Systematics", " Phylogenetics and taxonomy", "equatorial glacier foreland", "Equatorial glacier foreland", "Glacier retreat", "Molecular Biology", "Biology", "Demography", "Marine Microbial Diversity and Biogeography", "Colonisation", "South America", "15. Life on land", "[SDE.BE] Environmental Sciences/Biodiversity and Ecology", "13. Climate action", "FOS: Biological sciences", "[SDV.EE.ECO]Life Sciences [q-bio]/Ecology", "Environmental Science", "[SDE.BE]Environmental Sciences/Biodiversity and Ecology", "early succession; equatorial glacier foreland; glacier retreat; multiple-taxa", "Environmental DNA in Biodiversity Monitoring"]}, "links": [{"href": "https://air.unimi.it/bitstream/2434/851699/2/rosero%202021.pdf"}, {"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/ecog.05478"}, {"href": "https://doi.org/10.1111/ecog.05478"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Ecography", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1111/ecog.05478", "name": "item", "description": "10.1111/ecog.05478", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1111/ecog.05478"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-05-06T00:00:00Z"}}, {"id": "10.1098/rstb.2020.0169", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:19:10Z", "type": "Journal Article", "created": "2021-08-08", "title": "The role of soils in delivering Nature's Contributions to People", "description": "

This theme issue provides an assessment of the contribution of soils to Nature's Contributions to People (NCP). The papers in this issue show that soils can contribute positively to the delivery of all NCP. These contributions can be maximized through careful soil management to provide healthy soils, but poorly managed, degraded or polluted soils may contribute negatively to the delivery of NCP. Soils are also shown to contribute positively to the UN Sustainable Development Goals. Papers in the theme issue emphasize the need for careful soil management. Priorities for soil management must include: (i) for healthy soils in natural ecosystems, protect them from conversion and degradation, (ii) for managed soils, manage in a way to protect and enhance soil biodiversity, health, productivity and sustainability and to prevent degradation, and (iii) for degraded soils, restore to full soil health. Our knowledge of what constitutes sustainable soil management is mature enough to implement best management practices, in order to maintain and improve soil health. The papers in this issue show the vast potential of soils to contribute to NCP. This is not only desirable, but essential to sustain a healthy planet and if we are to deliver sustainable development in the decades to come.

This article is part of the theme issue \uffe2\uff80\uff98The role of soils in delivering Nature\uffe2\uff80\uff99s Contributions to People\uffe2\uff80\uff99.MicroRNAs (miRNAs) are a class of small non-coding RNAs that play important regulatory roles in plant genomes. While some miRNA genes are deeply conserved, the majority appear to be species-specific, raising the question of how they emerge and integrate into cellular regulatory networks. To address this question, we first performed a detailed annotation of miRNA genes in the closely related Arabidopsis halleri and A. lyrata, then evaluated their phylogenetic conservation across 87 plant species. We then characterized the process by which newly emerged miRNA genes progressively acquire the properties of \uffe2\uff80\uff9ccanonical\uffe2\uff80\uff9d miRNA genes, in terms of size and stability of the hairpin precursor, loading of their cleavage products into Argonaute proteins, and potential to regulate downstream target genes. Analysis of nucleotide polymorphism distribution along the hairpin sequence (stem, mature miRNA, terminal loop) revealed that the selective constraints on recently emerged miRNA genes were initially weak, gradually increasing toward evolutionarily conserved miRNA genes. Our results illustrate the rapid birth-and-death of miRNA genes in plant genomes, and provide a detailed picture of the evolutionary progression toward canonical miRNAs by which a small fraction of de novo formed miRNA genes eventually integrate into \uffe2\uff80\uff9ccore\uffe2\uff80\uff9d biological processes.Investigating active participants in the fixation of dinitrogen gas is vital as N is often a limiting factor for primary production. Biological nitrogen fixation is performed by a diverse guild of bacteria and archaea (diazotrophs), which can be free\uffe2\uff80\uff90living or symbionts. Free\uffe2\uff80\uff90living diazotrophs are widely distributed in the environment, yet our knowledge about their identity and ecophysiology is still limited. A major challenge in investigating this guild is inferring activity from genetic data as this process is highly regulated. To address this challenge, we evaluated and improved several 15N\uffe2\uff80\uff90based methods for detecting N2 fixation activity (with a focus on soil samples) and studying active diazotrophs. We compared the acetylene reduction assay and the 15N2 tracer method and demonstrated that the latter is more sensitive in samples with low activity. Additionally, tracing 15N into microbial RNA provides much higher sensitivity compared to bulk soil analysis. Active soil diazotrophs were identified with a 15N\uffe2\uff80\uff90RNA\uffe2\uff80\uff90SIP approach optimized for environmental samples and benchmarked to 15N\uffe2\uff80\uff90DNA\uffe2\uff80\uff90SIP. Lastly, we investigated the feasibility of using SIP\uffe2\uff80\uff90Raman microspectroscopy for detecting 15N\uffe2\uff80\uff90labelled cells. Taken together, these tools allow identifying and investigating active free\uffe2\uff80\uff90living diazotrophs in a highly sensitive manner in diverse environments, from bulk to the single\uffe2\uff80\uff90cell level.Biochar amendment is one of the most promising agricultural approaches to tackle climate change by enhancing soil carbon (C) sequestration. Microbial\uffe2\uff80\uff90mediated decomposition processes are fundamental for the fate and persistence of sequestered C in soil, but the underlying mechanisms are uncertain. Here, we synthesise 923 observations regarding the effects of biochar addition (over periods ranging from several weeks to several years) on soil C\uffe2\uff80\uff90degrading enzyme activities from 130 articles across five continents worldwide. Our results showed that biochar addition increased soil ligninase activity targeting complex phenolic macromolecules by 7.1%, but suppressed cellulase activity degrading simpler polysaccharides by 8.3%. These shifts in enzyme activities explained the most variation of changes in soil C sequestration across a wide range of climatic, edaphic and experimental conditions, with biochar\uffe2\uff80\uff90induced shift in ligninase:cellulase ratio correlating negatively with soil C sequestration. Specifically, short\uffe2\uff80\uff90term (<1\uffc2\uffa0year) biochar addition significantly reduced cellulase activity by 4.6% and enhanced soil organic C sequestration by 87.5%, whereas no significant responses were observed for ligninase activity and ligninase:cellulase ratio. However, long\uffe2\uff80\uff90term (\uffe2\uff89\uffa51\uffc2\uffa0year) biochar addition significantly enhanced ligninase activity by 5.2% and ligninase:cellulase ratio by 36.1%, leading to a smaller increase in soil organic C sequestration (25.1%). These results suggest that shifts in enzyme activities increased ligninase:cellulase ratio with time after biochar addition, limiting long\uffe2\uff80\uff90term soil C sequestration with biochar addition. Our work provides novel evidence to explain the diminished soil C sequestration with long\uffe2\uff80\uff90term biochar addition and suggests that earlier studies may have overestimated soil C sequestration with biochar addition by failing to consider the physiological acclimation of soil microorganisms over time.

How the multiple facets of soil fungal diversity vary worldwide remains virtually unknown, hindering the management of this essential species-rich group. By sequencing high-resolution DNA markers in over 4000 topsoil samples from natural and human-altered ecosystems across all continents, we illustrate the distributions and drivers of different levels of taxonomic and phylogenetic diversity of fungi and their ecological groups. We show the impact of precipitation and temperature interactions on local fungal species richness (alpha diversity) across different climates. Our findings reveal how temperature drives fungal compositional turnover (beta diversity) and phylogenetic diversity, linking them with regional species richness (gamma diversity). We integrate fungi into the principles of global biodiversity distribution and present detailed maps for biodiversity conservation and modeling of global ecological processes.

", "keywords": ["Supplementary Data", "biodiversity", " fungi", " ecology", "QH301 Biology", "Diversity (politics)", "Plant Science", "Biodiversity conservation", "Fungal Diversity", "Agricultural and Biological Sciences", "Soil", "Life", "Sociology", "WATER", "Global biodiversity distribution", "Fungal diversity", "Phylogeny", "Soil Microbiology", "2. Zero hunger", "Multidisciplinary", "Earth", " Environmental", " Ecological", " and Space Sciences", "Geography", "Ecology", "soil fungal diversity", "4. Education", "SPECIES RICHNESS", "Life Sciences", "https://www.science.org/doi/suppl/10.1126/sciadv.adj8016/suppl_file/sciadv.adj8016_sm.pdf", "Biodiversity", "FOS: Sociology", "global biodiversity distribution", "sienet", "https://www.science.org/doi/suppl/10.1126/sciadv.adj8016/suppl_file/sciadv.adj8016_tables_s1_to_s13.zip", "Diversity and Evolution of Fungal Pathogens", "570", "Supplementary Information", "DNA markers", "QH301", "Sequencing high-resolution DNA", "Biochemistry", " Genetics and Molecular Biology", "monimuotoisuus", "Mycorrhizal Fungi and Plant Interactions", "Life Science", "Humans", "14. Life underwater", "General", "Global ecological processes", "Biology", "Ecosystem", "Ecology", " Evolution", " Behavior and Systematics", "global ecological processes", "Soil fungal diversity", "microbiology", "Fungi", "Water", "Cell Biology", "15. Life on land", "luonnon monimuotoisuus", "Agronomy", "biodiversiteetti", "LIFE", "ekosysteemit (ekologia)", "Evolution and Ecology of Endophyte-Grass Symbiosis", "13. Climate action", "Ecology", " evolutionary biology", "Earth and Environmental Sciences", "FOS: Biological sciences", "Anthropology", "ta1181", "biodiversity conservation", "Species richness"]}, "links": [{"href": "https://www.science.org/doi/epdf/10.1126/sciadv.adj8016"}, {"href": "https://www.science.org/doi/pdf/10.1126/sciadv.adj8016"}, {"href": "https://doi.org/10.1126/sciadv.adj8016"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Science%20Advances", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1126/sciadv.adj8016", "name": "item", "description": "10.1126/sciadv.adj8016", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1126/sciadv.adj8016"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-12-01T00:00:00Z"}}, {"id": "10.1371/journal.pone.0029642", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:20:12Z", "type": "Journal Article", "created": "2012-01-04", "title": "Carbon Stocks And Fluxes In Tropical Lowland Dipterocarp Rainforests In Sabah, Malaysian Borneo", "description": "Deforestation in the tropics is an important source of carbon C release to the atmosphere. To provide a sound scientific base for efforts taken to reduce emissions from deforestation and degradation (REDD+) good estimates of C stocks and fluxes are important. We present components of the C balance for selectively logged lowland tropical dipterocarp rainforest in the Malua Forest Reserve of Sabah, Malaysian Borneo. Total organic C in this area was 167.9 Mg C ha\u207b\u00b9\u00b13.8 (SD), including: Total aboveground (TAGC: 55%; 91.9 Mg C ha\u207b\u00b9\u00b12.9 SEM) and belowground carbon in trees (TBGC: 10%; 16.5 Mg C ha\u207b\u00b9\u00b10.5 SEM), deadwood (8%; 13.2 Mg C ha\u207b\u00b9\u00b13.5 SEM) and soil organic matter (SOM: 24%; 39.6 Mg C ha\u207b\u00b9\u00b10.9 SEM), understory vegetation (3%; 5.1 Mg C ha\u207b\u00b9\u00b11.7 SEM), standing litter (<1%; 0.7 Mg C ha\u207b\u00b9\u00b10.1 SEM) and fine root biomass (<1%; 0.9 Mg C ha\u207b\u00b9\u00b10.1 SEM). Fluxes included litterfall, a proxy for leaf net primary productivity (4.9 Mg C ha\u207b\u00b9 yr\u207b\u00b9\u00b10.1 SEM), and soil respiration, a measure for heterotrophic ecosystem respiration (28.6 Mg C ha\u207b\u00b9 yr\u207b\u00b9\u00b11.2 SEM). The missing estimates necessary to close the C balance are wood net primary productivity and autotrophic respiration.Twenty-two years after logging TAGC stocks were 28% lower compared to unlogged forest (128 Mg C ha\u207b\u00b9\u00b113.4 SEM); a combined weighted average mean reduction due to selective logging of -57.8 Mg C ha\u207b\u00b9 (with 95% CI -75.5 to -40.2). Based on the findings we conclude that selective logging decreased the dipterocarp stock by 55-66%. Silvicultural treatments may have the potential to accelerate the recovery of dipterocarp C stocks to pre-logging levels.", "keywords": ["0106 biological sciences", "1000 Multidisciplinary", "Tropical Climate", "Science", "Rain", "Q", "R", "1100 General Agricultural and Biological Sciences", "Biodiversity", "15. Life on land", "01 natural sciences", "Carbon", "Dipterocarpaceae", "Trees", "10127 Institute of Evolutionary Biology and Environmental Studies", "Soil", "1300 General Biochemistry", " Genetics and Molecular Biology", "Borneo", "Seedlings", "13. Climate action", "570 Life sciences; biology", "590 Animals (Zoology)", "Medicine", "Biomass", "Research Article"], "contacts": [{"organization": "Saner, Philippe, Loh, Yen Yee, Ong, Robert C., Hector, Andy,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.1371/journal.pone.0029642"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/PLoS%20ONE", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1371/journal.pone.0029642", "name": "item", "description": "10.1371/journal.pone.0029642", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1371/journal.pone.0029642"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2012-01-03T00:00:00Z"}}, {"id": "10.1128/msystems.00859-24", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:19:55Z", "type": "Journal Article", "created": "2024-09-10", "title": "A novel barcoded nanopore sequencing workflow of high-quality, full-length bacterial 16S amplicons for taxonomic annotation of bacterial isolates and complex microbial communities", "description": "ABSTRACT

Due to recent improvements, Nanopore sequencing has become a promising method for experiments relying on amplicon sequencing. We describe a flexible workflow to generate and annotate high-quality, full-length 16S rDNA amplicons. We evaluated it for two applications, namely, (i) identification of bacterial isolates and (ii) species-level profiling of microbial communities. We assessed the identification of single bacterial isolates by sequencing, using a set of barcoded full-length 16S rRNA gene primer pairs (pair A), on 47 isolates encompassing multiple genera and compared those results with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based identification. Species-level community profiling was tested with two sets of barcoded full-length 16S primer pairs (A and B) and compared to the results obtained with shotgun Illumina sequencing using 27 stool samples. We developed a Nextflow pipeline to retain high-quality reads and taxonomically annotate them. We found high agreement between our workflow and MALDI-TOF data for isolate identification (positive predictive value = 0.90, Cram\uffc3\uffa9r\uffe2\uff80\uff99s V = 0.857, and Theil\uffe2\uff80\uff99s U = 0.316). For species-level community profiling, we found strong correlations ( r s > 0.6) of alpha diversity indices between the two primer sets and Illumina sequencing. At the community level, we found significant but small differences when comparing sequencing techniques. Finally, we found a moderate to strong correlation when comparing the relative abundances of individual species (average r s = 0.6 and 0.533 for primers A and B). Despite identified shortcomings, the proposed workflow enabled accurate identification of single bacterial isolates and prominent features in microbial communities, making it a worthwhile alternative to MALDI-TOF MS and Illumina sequencing.

IMPORTANCE

A quick, robust, simple, and cost-effective method to identify bacterial isolates and communities in each sample is indispensable in the fields of microbiology and infection biology. Recent technological advances in Oxford Nanopore Technologies sequencing make this technique an attractive option considering the adaptability, portability, and cost-effectiveness of the platform, even with small sequencing batches. Here, we validated a flexible workflow to identify bacterial isolates and characterize bacterial communities using the Oxford Nanopore Technologies sequencing platform combined with the most recent v14 chemistry kits. For bacterial isolates, we compared our nanopore-based approach to matrix-assisted laser desorption ionization-time of flight mass spectrometry-based identification. For species-level profiling of complex bacterial communities, we compared our nanopore-based approach to Illumina shotgun sequencing. For reproducibility purposes, we wrapped the code used to process the sequencing data into a ready-to-use and self-contained Nextflow pipeline.

", "keywords": ["DNA", " Bacterial", "1303 Biochemistry", "gut microbiome", "610 Medicine & health", "Microbiology", "Workflow", "1311 Genetics", "RNA", " Ribosomal", " 16S", "1312 Molecular Biology", "1706 Computer Science Applications", "DNA Barcoding", " Taxonomic", "Humans", "DNA sequencing", "Bacteria", "10179 Institute of Medical Microbiology", "Microbiota", "2404 Microbiology", "1314 Physiology", "bioinformatics", "QR1-502", "Nanopore Sequencing", "1105 Ecology", " Evolution", " Behavior and Systematics", "Spectrometry", " Mass", " Matrix-Assisted Laser Desorption-Ionization", "570 Life sciences; biology", "2611 Modeling and Simulation", "Research Article"]}, "links": [{"href": "https://doi.org/10.1128/msystems.00859-24"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/mSystems", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.1128/msystems.00859-24", "name": "item", "description": "10.1128/msystems.00859-24", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.1128/msystems.00859-24"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2024-04-11T00:00:00Z"}}, {"id": "10.3389/fpls.2022.922982", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:21:39Z", "type": "Journal Article", "created": "2022-06-23", "title": "Molecular Mechanisms of Intercellular Rhizobial Infection: Novel Findings of an Ancient Process", "description": "

Establishment of the root-nodule symbiosis in legumes involves rhizobial infection of nodule primordia in the root cortex that is dependent on rhizobia crossing the root epidermal barrier. Two mechanisms have been described: either through root hair infection threads or through the intercellular passage of bacteria. Among the legume genera investigated, around 75% use root hair entry and around 25% the intercellular entry mode. Root-hair infection thread-mediated infection has been extensively studied in the model legumes Medicago truncatula and Lotus japonicus. In contrast, the molecular circuit recruited during intercellular infection, which is presumably an ancient and simpler pathway, remains poorly known. In recent years, important discoveries have been made to better understand the transcriptome response and the genetic components involved in legumes with obligate (Aeschynomene and Arachis spp.) and conditional (Lotus and Sesbania spp.) intercellular rhizobial infections. This review addresses these novel findings and briefly considers possible future research to shed light on the molecular players that orchestrate intercellular infection in legumes.

Abstract. In the age of big data, soil data are more available than ever, but -outside of a few large soil survey resources- remain largely unusable for informing soil management and understanding Earth system processes outside of the original study. Data science has promised a fully reusable research pipeline where data from past studies are used to contextualize new findings and reanalyzed for global relevance. Yet synthesis projects encounter challenges at all steps of the data reuse pipeline, including unavailable data, labor-intensive transcription of datasets, incomplete metadata, and a lack of communication between collaborators. Here, using insights from a diversity of soil, data and climate scientists, we summarize current practices in soil data synthesis across all stages of database creation: data discovery, input, harmonization, curation, and publication. We then suggest new soil-focused semantic tools to improve existing data pipelines, such as ontologies, vocabulary lists, and community practices. Our goal is to provide the soil data community with an overview of current practices in soil data and where we need to go to fully leverage big data to solve soil problems in the next century.

", "keywords": ["FOS: Computer and information sciences", "Data Sharing", "Biomedical Ontologies and Text Mining", "Data management", "Leverage (statistics)", "01 natural sciences", "Data science", "Data Sharing and Stewardship in Science", "Database", "Big data", "Biochemistry", " Genetics and Molecular Biology", "Machine learning", "Molecular Biology", "Data mining", "0105 earth and related environmental sciences", "2. Zero hunger", "Metadata", "Ecology", "Data curation", "Physics", "Life Sciences", "Acoustics", "15. Life on land", "Computer science", "World Wide Web", "Harmonization", "13. Climate action", "FOS: Biological sciences", "Computer Science", "Physical Sciences", "Environmental Science", "Data Reuse", "Environmental DNA in Biodiversity Monitoring", "Information Systems"]}, "links": [{"href": "https://doi.org/10.5194/bg-2021-323"}, {"rel": "self", "type": "application/geo+json", "title": "10.5194/bg-2021-323", "name": "item", "description": "10.5194/bg-2021-323", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5194/bg-2021-323"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2021-12-15T00:00:00Z"}}, {"id": "10.5194/bg-3-571-2006", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:22:41Z", "type": "Journal Article", "created": "2010-04-29", "description": "

Abstract. Eddy covariance technique to measure CO2, water and energy fluxes between biosphere and atmosphere is widely spread and used in various regional networks. Currently more than 250 eddy covariance sites are active around the world measuring carbon exchange at high temporal resolution for different biomes and climatic conditions. In this paper a new standardized set of corrections is introduced and the uncertainties associated with these corrections are assessed for eight different forest sites in Europe with a total of 12 yearly datasets. The uncertainties introduced on the two components GPP (Gross Primary Production) and TER (Terrestrial Ecosystem Respiration) are also discussed and a quantitative analysis presented. Through a factorial analysis we find that generally, uncertainties by different corrections are additive without interactions and that the heuristic u*-correction introduces the largest uncertainty. The results show that a standardized data processing is needed for an effective comparison across biomes and for underpinning inter-annual variability. The methodology presented in this paper has also been integrated in the European database of the eddy covariance measurements.

", "keywords": ["european database of the eddy covariance measurements", "550", "net ecosystem exchange", "Molecular Biology/Biochemistry [q-bio.BM]", "[SDU.STU]Sciences of the Universe [physics]/Earth Sciences", "[SDU.ASTR] Sciences of the Universe [physics]/Astrophysics [astro-ph]", "[PHYS.ASTR.CO]Physics [physics]/Astrophysics [astro-ph]/Cosmology and Extra-Galactic Astrophysics [astro-ph.CO]", "Life", "QH501-531", "[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry", " Molecular Biology/Biochemistry [q-bio.BM]", "QH540-549.5", "eddy covariance technique", "[SDU.OCEAN]Sciences of the Universe [physics]/Ocean", "QE1-996.5", "algorithm", "[SDU.ASTR]Sciences of the Universe [physics]/Astrophysics [astro-ph]", "Ecology", "Atmosphere", "[SDU.OCEAN] Sciences of the Universe [physics]/Ocean", " Atmosphere", "500", "Geology", "15. Life on land", "terrestrial ecosystem respiration", "gross primary production", "[SDU.ENVI] Sciences of the Universe [physics]/Continental interfaces", " environment", "[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry", "[PHYS.ASTR.CO] Physics [physics]/Astrophysics [astro-ph]/Cosmology and Extra-Galactic Astrophysics [astro-ph.CO]", "13. Climate action", "[SDU.STU] Sciences of the Universe [physics]/Earth Sciences", "co2", "measurement", "[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces", "environment"]}, "links": [{"href": "https://doi.org/10.5194/bg-3-571-2006"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Biogeosciences", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5194/bg-3-571-2006", "name": "item", "description": "10.5194/bg-3-571-2006", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5194/bg-3-571-2006"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2006-11-27T00:00:00Z"}}, {"id": "10.5194/egusphere-egu21-5218", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:22:45Z", "type": "Journal Article", "created": "2021-03-04", "title": "Microbial inputs at the litter layer translate climate into altered organic matter properties", "description": "

&lt;p&gt;Plant litter chemistry is altered during decomposition but it remains unknown if these alterations, and thus the composition of residual litter, will change in response to climate. Selective microbial mineralization of litter components and the accumulation of microbial necromass can drive litter compositional change, but the extent to which these mechanisms respond to climate remains poorly understood. We addressed this knowledge gap by studying needle litter decomposition along a boreal forest climate transect. Specifically, we investigated how the composition and/or metabolism of the decomposer community varies with climate, and if that variation is associated with distinct modifications of litter chemistry during decomposition. We analyzed the composition of microbial phospholipid fatty acids (PLFAs) in the litter layer and measured natural abundance &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C&lt;sub&gt;PLFA&lt;/sub&gt; values as an integrated measure of microbial metabolisms. Changes in litter chemistry and &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C values were measured in litterbag experiments conducted at each transect site. A warmer climate was associated with higher litter nitrogen concentrations as well as altered microbial community structure (lower fungi:bacteria ratios) and microbial metabolism (higher &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C&lt;sub&gt;PLFA&lt;/sub&gt;). Litter in warmer transect regions accumulated less aliphatic&amp;#8208;C (lipids, waxes) and retained more O&amp;#8208;alkyl&amp;#8208;C (carbohydrates), consistent with enhanced &lt;sup&gt;13&lt;/sup&gt;C&amp;#8208;enrichment in residual litter, than in colder regions. These results suggest that chemical changes during litter decomposition will change with climate, driven primarily by indirect climate effects (e.g., greater nitrogen availability and decreased fungi:bacteria ratios) rather than direct temperature effects. A positive correlation between microbial biomass &amp;#948;&lt;sup&gt;13&lt;/sup&gt;C values and &lt;sup&gt;13&lt;/sup&gt;C&amp;#8208;enrichment during decomposition suggests that change in litter chemistry is driven more by distinct microbial necromass inputs than differences in the selective removal of litter components. Our study highlights the role that microbial inputs during early litter decomposition can play in shaping surface litter contribution to soil organic matter as it responds to climate warming effects such as greater nitrogen availability.&lt;/p&gt;

", "keywords": ["DECOMPOSITION", "C-13", "CP‐", "necromass", "litter decomposition", "COMMUNITY COMPOSITION", "Soil", "CARBON SEQUESTRATION", "Taiga", "boreal forest", "bacteria", "C-13 NMR", "TEMPERATURE", "Biochemistry", " cell and molecular biology", "Soil Microbiology", "FUNGAL", "2. Zero hunger", "MAS C-13‐", "Fungi", "04 agricultural and veterinary sciences", "15. Life on land", "NMR", "6. Clean water", "climate transect", "Plant Leaves", "13. Climate action", "FOREST SOILS", "PLFA", "0401 agriculture", " forestry", " and fisheries", "fungi", "FATTY-ACIDS", "BULK CARBON", "LIGNIN"]}, "links": [{"href": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/gcb.15420"}, {"href": "https://doi.org/10.5194/egusphere-egu21-5218"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Global%20Change%20Biology", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.5194/egusphere-egu21-5218", "name": "item", "description": "10.5194/egusphere-egu21-5218", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.5194/egusphere-egu21-5218"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-11-16T00:00:00Z"}}, {"id": "10.60692/aqpen-xja81", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:25:25Z", "type": "Journal Article", "created": "2022-12-22", "title": "Litter chemistry of common European tree species drives the feeding preference and consumption rate of soil invertebrates, and shapes the diversity and structure of gut and faecal microbiomes", "description": "Open AccessTerrestrial isopods and millipedes are key drivers of a litter decomposition in terrestrial ecosystems but the effects of litter chemistry on feeding preference and litter consumption rate as well as on the diversity and composition of gut and faecal microbiome still entails several challenges. We established a mesocosm experiment with terrestrial isopods (Oniscus asellus) and millipedes (Glomeris marginata) fed by leaf litter from six common European tree species (ash, maple, lime, beech, oak and Norway spruce) to reveal the effect of litter chemistry on consumption rate and feeding preference as well as on the compositions of gut and faecal microbiomes. The total percentage of consumed litter showed that O. asellus preferred nutrient-rich and labile-C litter of ash over more recalcitrant litter of oak, beech, and Norway spruce, while G. marginata preferred calcium-rich ash, maple and lime litter over beech and Norway spruce. Consumption of litter by O. asellus and G. marginata increased with concentrations of magnesium, sulphur and potassium but decreased with concentrations of iron, phosphorus, lignin, cellulose and TOC. The millipede G. marginata harboured higher bacterial OTU richness (73.5 \u00b1 12.5) than the isopod O. asellus (49.1 \u00b1 15.9), but fungal OTU richness was similar with 25.8 \u00b1 6.7 in O. asellus and 25.7 \u00b1 2.7 in G. marginata. In total, faeces of both animals hosted higher diversity than gut. In gut and faeces of O. asellus, the fungal OTU richness was highest for individuals fed by litter of Norway spruce, while lowest OTU richness was recorded for individuals fed by litter of more palatable ash. In contrast, the highest diversity of the fungal community in gut and faeces of G. marginata was recorded for individuals fed by palatable lime litter, while the lowest OTUs richness was recorded when millipedes were fed by maple and spruce. The structures of bacterial and fungal communities generally separated between O. asellus and G. marginata. The fungal community structure in gut and faeces differed between animals fed by different foliar litters, while the bacterial community structure mainly differed between gut and faeces regardless of the offered type of litter. The fungal community structure in gut and faeces of O. asellus and G. marginata were shaped by concentrations of magnesium, sulphur, lignin and cellulose. The bacterial communities in gut and faeces of both O. asellus and G. marginata were dominated by copiotrophic bacteria, while fungal communities were dominated by unspecified saprotrophs. Our study suggest that litter quality is a strong driver of feeding preference and consumption rates as well as composition of bacterial and fungal communities in gut and faeces of two species representing the main groups of litter feeding soil fauna in European forests.", "keywords": ["0301 basic medicine", "Genomic Insights into Social Insects and Symbiosis", "Plant Science", "Plant litter", "Agricultural and Biological Sciences", "03 medical and health sciences", "Biochemistry", " Genetics and Molecular Biology", "Litter", "Genetics", "Ecological Niche", "Biology", "Ecosystem", "Beech", "0303 health sciences", "Species Distribution Modeling and Climate Change Impacts", "Ecology", "Ecological Modeling", "Botany", "Life Sciences", "15. Life on land", "Plant-Parasitic Nematodes in Molecular Plant Pathology", "Detritus", "FOS: Biological sciences", "Detritivore", "Environmental Science", "Physical Sciences", "Species richness"]}, "links": [{"href": "https://doi.org/10.60692/aqpen-xja81"}, {"rel": "related", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/Soil%20Biology%20and%20Biochemistry", "name": "related record", "description": "related record", "type": "application/json"}, {"rel": "self", "type": "application/geo+json", "title": "10.60692/aqpen-xja81", "name": "item", "description": "10.60692/aqpen-xja81", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.60692/aqpen-xja81"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2023-02-01T00:00:00Z"}}, {"id": "10.6084/m9.figshare.11925039", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-27T16:25:27Z", "type": "Dataset", "created": "2020-03-03", "title": "Additional file 3 of Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics", "description": "Additional file 3. Statistics of the obtained and processed metagenome and metatranscriptome sequences.", "keywords": ["Ecology", "FOS: Biological sciences", "Genetics", "110309 Infectious Diseases", "Marine Biology", "FOS: Health sciences", "Microbiology", "Molecular Biology", "69999 Biological Sciences not elsewhere classified"], "contacts": [{"organization": "Maus, Irena, Klocke, Michael, Derenk\u00f3, Jaqueline, Stolze, Yvonne, Beckstette, Michael, Jost, Carsten, Wibberg, Daniel, Blom, Jochen, Henke, Christian, Willenb\u00fccher, Katharina, Rumming, Madis, Rademacher, Antje, P\u00fchler, Alfred, Sczyrba, Alexander, Schl\u00fcter, Andreas,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.6084/m9.figshare.11925039"}, {"rel": "self", "type": "application/geo+json", "title": "10.6084/m9.figshare.11925039", "name": "item", "description": "10.6084/m9.figshare.11925039", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.6084/m9.figshare.11925039"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-01T00:00:00Z"}}, {"id": "10.6084/m9.figshare.11925039.v1", "type": "Feature", "geometry": null, "properties": {"license": "Open Access", "updated": "2026-07-27T16:25:27Z", "type": "Dataset", "created": "2020-03-03", "title": "Additional file 3 of Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics", "description": "Additional file 3. Statistics of the obtained and processed metagenome and metatranscriptome sequences.", "keywords": ["Ecology", "FOS: Biological sciences", "Genetics", "110309 Infectious Diseases", "Marine Biology", "FOS: Health sciences", "Microbiology", "Molecular Biology", "69999 Biological Sciences not elsewhere classified"], "contacts": [{"organization": "Maus, Irena, Klocke, Michael, Derenk\u00f3, Jaqueline, Stolze, Yvonne, Beckstette, Michael, Jost, Carsten, Wibberg, Daniel, Blom, Jochen, Henke, Christian, Willenb\u00fccher, Katharina, Rumming, Madis, Rademacher, Antje, P\u00fchler, Alfred, Sczyrba, Alexander, Schl\u00fcter, Andreas,", "roles": ["creator"]}]}, "links": [{"href": "https://doi.org/10.6084/m9.figshare.11925039.v1"}, {"rel": "self", "type": "application/geo+json", "title": "10.6084/m9.figshare.11925039.v1", "name": "item", "description": "10.6084/m9.figshare.11925039.v1", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main/items/10.6084/m9.figshare.11925039.v1"}, {"rel": "collection", "type": "application/json", "title": "Collection", "name": "collection", "description": "Collection", "href": "https://repository.soilwise-he.eu/cat/collections/metadata:main"}], "time": {"date": "2020-01-01T00:00:00Z"}}, {"id": "2764305888", "type": "Feature", "geometry": null, "properties": {"updated": "2026-07-27T16:27:11Z", "type": "Journal Article", "created": "2022-10-18", "title": "Application of stable\u2010isotope labelling techniques for the detection of active diazotrophs", "description": "Summary

Investigating active participants in the fixation of dinitrogen gas is vital as N is often a limiting factor for primary production. Biological nitrogen fixation is performed by a diverse guild of bacteria and archaea (diazotrophs), which can be free\uffe2\uff80\uff90living or symbionts. Free\uffe2\uff80\uff90living diazotrophs are widely distributed in the environment, yet our knowledge about their identity and ecophysiology is still limited. A major challenge in investigating this guild is inferring activity from genetic data as this process is highly regulated. To address this challenge, we evaluated and improved several 15N\uffe2\uff80\uff90based methods for detecting N2 fixation activity (with a focus on soil samples) and studying active diazotrophs. We compared the acetylene reduction assay and the 15N2 tracer method and demonstrated that the latter is more sensitive in samples with low activity. Additionally, tracing 15N into microbial RNA provides much higher sensitivity compared to bulk soil analysis. Active soil diazotrophs were identified with a 15N\uffe2\uff80\uff90RNA\uffe2\uff80\uff90SIP approach optimized for environmental samples and benchmarked to 15N\uffe2\uff80\uff90DNA\uffe2\uff80\uff90SIP. Lastly, we investigated the feasibility of using SIP\uffe2\uff80\uff90Raman microspectroscopy for detecting 15N\uffe2\uff80\uff90labelled cells. Taken together, these tools allow identifying and investigating active free\uffe2\uff80\uff90living diazotrophs in a highly sensitive manner in diverse environments, from bulk to the single\uffe2\uff80\uff90cell level.Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16\uffe2\uff80\uff93C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture conditions. This work addressed the elucidation of the genome sequence of MEP218 and its taxonomic classification. The genome comprises 3,944,892\uffc2\uffa0bp, with a total of 3474 coding sequences and a G\uffe2\uff80\uff89+\uffe2\uff80\uff89C content of 46.59%. Our phylogenetic analysis to determine the taxonomic position demonstrated that the assignment of the MEP218 strain to Bacillus velezensis species provides insights into its evolutionary context and potential functional attributes. The in silico genome analysis revealed eleven gene clusters involved in the synthesis of secondary metabolites, including non-ribosomal CLP (fengycins and surfactin), polyketides, terpenes, and bacteriocins. Furthermore, genes encoding phytase, involved in the release of phytic phosphate for plant and animal nutrition, or other enzymes such as cellulase, xylanase, and alpha 1\uffe2\uff80\uff934 glucanase were detected. In vitro antagonistic assays against Salmonella typhimurium, Acinetobacter baumanii, Escherichia coli, among others, demonstrated a broad spectrum of C16\uffe2\uff80\uff93C17 fengycin produced by MEP218. MEP218 genome sequence analysis expanded our understanding of the diversity and genetic relationships within the Bacillus genus and updated the Bacillus databases with its unique trait to produce antibacterial fengycins and its potential as a resource of biotechnologically useful enzymes.